                         SEQUENCE LISTING

<110>  The University of North Carolina at Charlotte
       OncoTab, Inc.
       Mukherjee, Pinku
 
<120>  TUMOR SPECIFIC ANTIBODY CONJUGATES AND USES THEREFOR

<130>  1276/5/13 PCT

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially synthesized peptide sequence


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  the T at residue number 5 can be N-glycosylated

<400>  1

Ser Leu Ala Pro Thr Val His Asn Val 
1               5                   


<210>  2
<211>  1255
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Thr Pro Gly Thr Gln Ser Pro Phe Phe Leu Leu Leu Leu Leu Thr 
1               5                   10                  15      


Val Leu Thr Val Val Thr Gly Ser Gly His Ala Ser Ser Thr Pro Gly 
            20                  25                  30          


Gly Glu Lys Glu Thr Ser Ala Thr Gln Arg Ser Ser Val Pro Ser Ser 
        35                  40                  45              


Thr Glu Lys Asn Ala Val Ser Met Thr Ser Ser Val Leu Ser Ser His 
    50                  55                  60                  


Ser Pro Gly Ser Gly Ser Ser Thr Thr Gln Gly Gln Asp Val Thr Leu 
65                  70                  75                  80  


Ala Pro Ala Thr Glu Pro Ala Ser Gly Ser Ala Ala Thr Trp Gly Gln 
                85                  90                  95      


Asp Val Thr Ser Val Pro Val Thr Arg Pro Ala Leu Gly Ser Thr Thr 
            100                 105                 110         


Pro Pro Ala His Asp Val Thr Ser Ala Pro Asp Asn Lys Pro Ala Pro 
        115                 120                 125             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    130                 135                 140                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
145                 150                 155                 160 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                165                 170                 175     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            180                 185                 190         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        195                 200                 205             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    210                 215                 220                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
225                 230                 235                 240 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                245                 250                 255     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            260                 265                 270         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        275                 280                 285             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    290                 295                 300                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
305                 310                 315                 320 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                325                 330                 335     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            340                 345                 350         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        355                 360                 365             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    370                 375                 380                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
385                 390                 395                 400 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                405                 410                 415     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            420                 425                 430         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        435                 440                 445             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    450                 455                 460                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
465                 470                 475                 480 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                485                 490                 495     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            500                 505                 510         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        515                 520                 525             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    530                 535                 540                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
545                 550                 555                 560 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                565                 570                 575     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            580                 585                 590         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        595                 600                 605             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    610                 615                 620                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
625                 630                 635                 640 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                645                 650                 655     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            660                 665                 670         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        675                 680                 685             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    690                 695                 700                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
705                 710                 715                 720 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                725                 730                 735     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            740                 745                 750         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        755                 760                 765             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    770                 775                 780                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
785                 790                 795                 800 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                805                 810                 815     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            820                 825                 830         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        835                 840                 845             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr 
    850                 855                 860                 


Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser 
865                 870                 875                 880 


Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His 
                885                 890                 895     


Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala 
            900                 905                 910         


Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro 
        915                 920                 925             


Gly Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Asn 
    930                 935                 940                 


Arg Pro Ala Leu Gly Ser Thr Ala Pro Pro Val His Asn Val Thr Ser 
945                 950                 955                 960 


Ala Ser Gly Ser Ala Ser Gly Ser Ala Ser Thr Leu Val His Asn Gly 
                965                 970                 975     


Thr Ser Ala Arg Ala Thr Thr Thr Pro Ala Ser Lys Ser Thr Pro Phe 
            980                 985                 990         


Ser Ile Pro Ser His His Ser Asp  Thr Pro Thr Thr Leu  Ala Ser His 
        995                 1000                 1005             


Ser Thr  Lys Thr Asp Ala Ser  Ser Thr His His Ser  Ser Val Pro 
    1010                 1015                 1020             


Pro Leu  Thr Ser Ser Asn His  Ser Thr Ser Pro Gln  Leu Ser Thr 
    1025                 1030                 1035             


Gly Val  Ser Phe Phe Phe Leu  Ser Phe His Ile Ser  Asn Leu Gln 
    1040                 1045                 1050             


Phe Asn  Ser Ser Leu Glu Asp  Pro Ser Thr Asp Tyr  Tyr Gln Glu 
    1055                 1060                 1065             


Leu Gln  Arg Asp Ile Ser Glu  Met Phe Leu Gln Ile  Tyr Lys Gln 
    1070                 1075                 1080             


Gly Gly  Phe Leu Gly Leu Ser  Asn Ile Lys Phe Arg  Pro Gly Ser 
    1085                 1090                 1095             


Val Val  Val Gln Leu Thr Leu  Ala Phe Arg Glu Gly  Thr Ile Asn 
    1100                 1105                 1110             


Val His  Asp Val Glu Thr Gln  Phe Asn Gln Tyr Lys  Thr Glu Ala 
    1115                 1120                 1125             


Ala Ser  Arg Tyr Asn Leu Thr  Ile Ser Asp Val Ser  Val Ser Asp 
    1130                 1135                 1140             


Val Pro  Phe Pro Phe Ser Ala  Gln Ser Gly Ala Gly  Val Pro Gly 
    1145                 1150                 1155             


Trp Gly  Ile Ala Leu Leu Val  Leu Val Cys Val Leu  Val Ala Leu 
    1160                 1165                 1170             


Ala Ile  Val Tyr Leu Ile Ala  Leu Ala Val Cys Gln  Cys Arg Arg 
    1175                 1180                 1185             


Lys Asn  Tyr Gly Gln Leu Asp  Ile Phe Pro Ala Arg  Asp Thr Tyr 
    1190                 1195                 1200             


His Pro  Met Ser Glu Tyr Pro  Thr Tyr His Thr His  Gly Arg Tyr 
    1205                 1210                 1215             


Val Pro  Pro Ser Ser Thr Asp  Arg Ser Pro Tyr Glu  Lys Val Ser 
    1220                 1225                 1230             


Ala Gly  Asn Gly Gly Ser Ser  Leu Ser Tyr Thr Asn  Pro Ala Val 
    1235                 1240                 1245             


Ala Ala  Ala Ser Ala Asn Leu  
    1250                 1255 


<210>  3
<211>  188
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Thr Glu Tyr Lys Leu Val Val Val Gly Ala Gly Gly Val Gly Lys 
1               5                   10                  15      


Ser Ala Leu Thr Ile Gln Leu Ile Gln Asn His Phe Val Asp Glu Tyr 
            20                  25                  30          


Asp Pro Thr Ile Glu Asp Ser Tyr Arg Lys Gln Val Val Ile Asp Gly 
        35                  40                  45              


Glu Thr Cys Leu Leu Asp Ile Leu Asp Thr Ala Gly Gln Glu Glu Tyr 
    50                  55                  60                  


Ser Ala Met Arg Asp Gln Tyr Met Arg Thr Gly Glu Gly Phe Leu Cys 
65                  70                  75                  80  


Val Phe Ala Ile Asn Asn Thr Lys Ser Phe Glu Asp Ile His His Tyr 
                85                  90                  95      


Arg Glu Gln Ile Lys Arg Val Lys Asp Ser Glu Asp Val Pro Met Val 
            100                 105                 110         


Leu Val Gly Asn Lys Cys Asp Leu Pro Ser Arg Thr Val Asp Thr Lys 
        115                 120                 125             


Gln Ala Gln Asp Leu Ala Arg Ser Tyr Gly Ile Pro Phe Ile Glu Thr 
    130                 135                 140                 


Ser Ala Lys Thr Arg Gln Gly Val Asp Asp Ala Phe Tyr Thr Leu Val 
145                 150                 155                 160 


Arg Glu Ile Arg Lys His Lys Glu Lys Met Ser Lys Asp Gly Lys Lys 
                165                 170                 175     


Lys Lys Lys Lys Ser Lys Thr Lys Cys Val Ile Met 
            180                 185             


<210>  4
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  4

Ser Thr Ala Pro Pro Val His Asn Val 
1               5                   


<210>  5
<211>  25
<212>  PRT
<213>  Homo sapiens

<400>  5

Ser Thr Ala Pro Pro Val His Asn Val Thr Ser Ala Pro Asp Thr Arg 
1               5                   10                  15      


Pro Ala Pro Gly Ser Thr Ala Pro Pro 
            20                  25  


<210>  6
<211>  354
<212>  DNA
<213>  Mus musculus

<400>  6
gaggtccagc tgcagcagtc tgggggtgaa cgggcaacac ctggggcctc agtgaagatg       60

tcctgcaaga cttctggcta cacctttact aactactgga tgcactgggt aaaacagagg      120

cctggacagg gtctggaatg gattggatac attaatccta gcagtggtta tactcagtac      180

aatcagaagt tcaaggacaa ggccacattg actgcagaca aatcctccag cacagcctac      240

atacaactaa gcagcctgac atctgaagac tctgcagtct attactgttc aacctactat      300

ggtgactact tgtttcctta ctggggccaa gggactctgg tcactgtctc tgca            354


<210>  7
<211>  118
<212>  PRT
<213>  Mus musculus

<400>  7

Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg Ala Thr Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala 
        115             


<210>  8
<211>  339
<212>  DNA
<213>  Mus musculus

<400>  8
gatgttttga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc       60

atctcttgca gatctagtca ggacattgta tatggtaatg gaaacaccta tttagaatgg      120

tacctgcaga aaccaggcca gtctccaaag ctcctgatct acaaagtttc caaccggttt      180

tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc      240

agcagagtgg aggctgagga tctgggagtt tattactgct ttcaaggttc acatgttccg      300

tacacgttcg gaggggggac caagctggaa ataaaacgg                             339


<210>  9
<211>  113
<212>  PRT
<213>  Mus musculus

<400>  9

Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly 
1               5                   10                  15      


Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly 
                85                  90                  95      


Ser His Val Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg 
    


<210>  10
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  10

Gly Tyr Thr Phe Thr Asn Tyr Trp 
1               5               


<210>  11
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  11

Ile Asn Pro Ser Ser Gly Tyr Thr 
1               5               


<210>  12
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  12

Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
1               5                   10      


<210>  13
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  13

Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr 
1               5                   10      


<210>  14
<211>  3
<212>  PRT
<213>  Mus musculus

<400>  14

Lys Val Ser 
1           


<210>  15
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  15

Phe Gln Gly Ser His Val Pro Tyr Thr 
1               5                   


<210>  16
<211>  1482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially synthesized human CAR open reading frame


<220>
<221>  CDS
<222>  (1)..(1482)

<400>  16
atg gcc ctg ccc gtg acc gcc ctg ctc ttg ccc ctg gcc ctt ctg ctc         48
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu           
1               5                   10                  15                

cac gcc gcc aga ccc gag gtg cag ctg cag cag agc gga ggc gag aga         96
His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg           
            20                  25                  30                    

gcc acc cct ggc gcc agc gtg aag atg agc tgc aag acc agc ggc tac        144
Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr           
        35                  40                  45                        

acc ttc acc aac tac tgg atg cac tgg gtg aag cag aga ccc ggc cag        192
Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln           
    50                  55                  60                            

ggc ctg gag tgg atc ggc tac atc aac cct agc tcc ggc tac acc cag        240
Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln           
65                  70                  75                  80            

tac aac cag aag ttc aag gac aag gcc acc ctg acc gcc gac aag agc        288
Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser           
                85                  90                  95                

tcc agc acc gcc tac atc cag ctg agc tcc ctg acc agc gag gac tcc        336
Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser           
            100                 105                 110                   

gcc gtg tac tat tgc agc acc tac tac ggc gac tac ctg ttc ccc tac        384
Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr           
        115                 120                 125                       

tgg ggc cag ggc acc ctg gtg acc gtg agc gcc ggc gga ggc gga agc        432
Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser           
    130                 135                 140                           

gga ggc ggc gga tcc gga gga ggc ggc agc gac gtg ctg atg acc cag        480
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln           
145                 150                 155                 160           

acc cct ctg agc ctg ccc gtg agc ctg ggc gac cag gcc agc atc agc        528
Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser           
                165                 170                 175               

tgc aga agc tcc cag gac atc gtg tac ggc aac gga aac acc tac ctg        576
Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu           
            180                 185                 190                   

gag tgg tac ctc cag aag ccc ggc cag agc ccc aag ctg ctg atc tac        624
Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr           
        195                 200                 205                       

aag gtg agc aac aga ttc agc ggc gtg ccc gac aga ttc agc ggc tcc        672
Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser           
    210                 215                 220                           

gga agc gga acc gac ttc acc ctg aag atc agc aga gtg gag gcc gag        720
Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu           
225                 230                 235                 240           

gac ctg ggc gtg tac tat tgc ttc cag ggc agc cac gtg ccc tac acc        768
Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr           
                245                 250                 255               

ttc ggc gga ggc acc aag ctg gag atc aag aga gcg gcc gct atc gag        816
Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu           
            260                 265                 270                   

gtg gag cag aag ctg atc agc gag gag gac ctg cta gac aat gag aag        864
Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys           
        275                 280                 285                       

agc aat gga acc att atc cat gtg aaa ggg aaa cac ctt tgt cca agt        912
Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser           
    290                 295                 300                           

ccc cta ttt ccc gga cct tct aag ccc ttt tgg gtg ctg gtg gtg gtt        960
Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val           
305                 310                 315                 320           

ggt gga gtc ctg gct tgc tat agc ttg cta gta aca gtg gcc ttt att       1008
Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile           
                325                 330                 335               

att ttc tgg gtg agg agt aag agg agc agg ctc ctg cac agt gac tac       1056
Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr           
            340                 345                 350                   

atg aac atg act ccc cgc cgc ccc ggg ccc acc cgc aag cat tac cag       1104
Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln           
        355                 360                 365                       

ccc tat gcc cca cca cgc gac ttc gca gcc tat cgc tcc aga gtg aag       1152
Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys           
    370                 375                 380                           

ttc agc agg agc gca gac gcc ccc gcg tac cag cag ggc cag aac cag       1200
Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln           
385                 390                 395                 400           

ctc tat aac gag ctc aat cta gga cga aga gag gag tac gat gtt ttg       1248
Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu           
                405                 410                 415               

gac aag aga cgt ggc cgg gac cct gag atg ggg gga aag ccg aga agg       1296
Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg           
            420                 425                 430                   

aag aac cct cag gaa ggc ctg tac aat gaa ctg cag aaa gat aag atg       1344
Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met           
        435                 440                 445                       

gcg gag gcc tac agt gag att ggg atg aaa ggc gag cgc cgg agg ggc       1392
Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly           
    450                 455                 460                           

aag ggg cac gat ggc ctt tac cag ggt ctc agt aca gcc acc aag gac       1440
Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp           
465                 470                 475                 480           

acc tac gac gcc ctt cac atg cag gcc ctg ccc cct cgc taa               1482
Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg                       
                485                 490                                   


<210>  17
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg 
            20                  25                  30          


Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln 
145                 150                 155                 160 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490             


<210>  18
<211>  1464
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially synthesized mouse CAR open reading frame


<220>
<221>  CDS
<222>  (1)..(1464)

<400>  18
atg gcc ctg ccc gtg acc gcc ctg ctc ttg ccc ctg gcc ctt ctg ctc         48
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu           
1               5                   10                  15                

cac gcc gcc aga ccc gag gtg cag ctg cag cag agc gga ggc gag aga         96
His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg           
            20                  25                  30                    

gcc acc cct ggc gcc agc gtg aag atg agc tgc aag acc agc ggc tac        144
Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr           
        35                  40                  45                        

acc ttc acc aac tac tgg atg cac tgg gtg aag cag aga ccc ggc cag        192
Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln           
    50                  55                  60                            

ggc ctg gag tgg atc ggc tac atc aac cct agc tcc ggc tac acc cag        240
Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln           
65                  70                  75                  80            

tac aac cag aag ttc aag gac aag gcc acc ctg acc gcc gac aag agc        288
Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser           
                85                  90                  95                

tcc agc acc gcc tac atc cag ctg agc tcc ctg acc agc gag gac tcc        336
Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser           
            100                 105                 110                   

gcc gtg tac tat tgc agc acc tac tac ggc gac tac ctg ttc ccc tac        384
Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr           
        115                 120                 125                       

tgg ggc cag ggc acc ctg gtg acc gtg agc gcc ggc gga ggc gga agc        432
Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser           
    130                 135                 140                           

gga ggc ggc gga tcc gga gga ggc ggc agc gac gtg ctg atg acc cag        480
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln           
145                 150                 155                 160           

acc cct ctg agc ctg ccc gtg agc ctg ggc gac cag gcc agc atc agc        528
Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser           
                165                 170                 175               

tgc aga agc tcc cag gac atc gtg tac ggc aac gga aac acc tac ctg        576
Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu           
            180                 185                 190                   

gag tgg tac ctc cag aag ccc ggc cag agc ccc aag ctg ctg atc tac        624
Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr           
        195                 200                 205                       

aag gtg agc aac aga ttc agc ggc gtg ccc gac aga ttc agc ggc tcc        672
Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser           
    210                 215                 220                           

gga agc gga acc gac ttc acc ctg aag atc agc aga gtg gag gcc gag        720
Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu           
225                 230                 235                 240           

gac ctg ggc gtg tac tat tgc ttc cag ggc agc cac gtg ccc tac acc        768
Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr           
                245                 250                 255               

ttc ggc gga ggc acc aag ctg gag atc aag aga gcg gcc gcc atc gag        816
Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu           
            260                 265                 270                   

ttc atg tac ccc ccc ccc tac ctg gac aac gag agg agc aac ggc acc        864
Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg Ser Asn Gly Thr           
        275                 280                 285                       

atc atc cac atc aag gag aag cac ctg tgc cac acc cag agc agc ccc        912
Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr Gln Ser Ser Pro           
    290                 295                 300                           

aag ctg ttc tgg gcc ctg gtg gtg gtg gcc ggc gtg ctg ttc tgc tac        960
Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val Leu Phe Cys Tyr           
305                 310                 315                 320           

ggc ctg ctg gtg acc gtg gcc ctg tgc gtg atc tgg acc aac agc agg       1008
Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr Asn Ser Arg           
                325                 330                 335               

agg aac agg ctg ctg cag agc gac tac atg aac atg acc ccc agg agg       1056
Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg Arg           
            340                 345                 350                   

ccc ggc ctg acc agg aag ccc tac cag ccc tac gcc ccc gcc agg gac       1104
Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg Asp           
        355                 360                 365                       

ttc gcc gcc tac agg ccc agg gcc aag ttc agc agg agc gcc gag acc       1152
Phe Ala Ala Tyr Arg Pro Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr           
    370                 375                 380                           

gcc gcc aac ctg cag gac ccc aac cag ctg tac aac gag ctg aac ctg       1200
Ala Ala Asn Leu Gln Asp Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu           
385                 390                 395                 400           

ggc agg agg gag gag tac gac gtg ctg gag aag aag agg gcc agg gac       1248
Gly Arg Arg Glu Glu Tyr Asp Val Leu Glu Lys Lys Arg Ala Arg Asp           
                405                 410                 415               

ccc gag atg ggc ggc aag cag cag agg agg agg aac ccc cag gag ggc       1296
Pro Glu Met Gly Gly Lys Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly           
            420                 425                 430                   

gtg tac aac gcc ctg cag aag gac aag atg gcc gag gcc tac agc gag       1344
Val Tyr Asn Ala Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu           
        435                 440                 445                       

atc ggc acc aag ggc gag agg agg agg ggc aag ggc cac gac ggc ctg       1392
Ile Gly Thr Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu           
    450                 455                 460                           

tac cag ggc ctg agc acc gcc acc aag gac acc tac gac gcc ctg cac       1440
Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His           
465                 470                 475                 480           

atg cag acc ctg gcc ccc agg tga                                       1464
Met Gln Thr Leu Ala Pro Arg                                               
                485                                                       


<210>  19
<211>  487
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  19

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg 
            20                  25                  30          


Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln 
145                 150                 155                 160 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Phe Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Arg Ser Asn Gly Thr 
        275                 280                 285             


Ile Ile His Ile Lys Glu Lys His Leu Cys His Thr Gln Ser Ser Pro 
    290                 295                 300                 


Lys Leu Phe Trp Ala Leu Val Val Val Ala Gly Val Leu Phe Cys Tyr 
305                 310                 315                 320 


Gly Leu Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr Asn Ser Arg 
                325                 330                 335     


Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro Ala Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Pro Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr 
    370                 375                 380                 


Ala Ala Asn Leu Gln Asp Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Glu Lys Lys Arg Ala Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly 
            420                 425                 430         


Val Tyr Asn Ala Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
        435                 440                 445             


Ile Gly Thr Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
    450                 455                 460                 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
465                 470                 475                 480 


Met Gln Thr Leu Ala Pro Arg 
                485         


<210>  20
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  20

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  21
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artifically synthesized peptide linker

<400>  21

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  22
<211>  98
<212>  PRT
<213>  Homo sapiens;

<400>  22

Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys 
1               5                   10                  15      


His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp 
            20                  25                  30          


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
        35                  40                  45              


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
    50                  55                  60                  


Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
65                  70                  75                  80  


Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
                85                  90                  95      


Arg Ser 
        


<210>  23
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  23

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  24
<211>  95
<212>  PRT
<213>  Mus musculus

<400>  24

Leu Asp Asn Glu Arg Ser Asn Gly Thr Ile Ile His Ile Lys Glu Lys 
1               5                   10                  15      


His Leu Cys His Thr Gln Ser Ser Pro Lys Leu Phe Trp Ala Leu Val 
            20                  25                  30          


Val Val Ala Gly Val Leu Phe Cys Tyr Gly Leu Leu Val Thr Val Ala 
        35                  40                  45              


Leu Cys Val Ile Trp Thr Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser 
    50                  55                  60                  


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro 
65                  70                  75                  80  


Tyr Gln Pro Tyr Ala Pro Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
                85                  90                  95  


<210>  25
<211>  27
<212>  PRT
<213>  Mus musculus

<400>  25

Phe Trp Ala Leu Val Val Val Ala Gly Val Leu Phe Cys Tyr Gly Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Leu Cys Val Ile Trp Thr 
            20                  25          


<210>  26
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  26

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  27
<211>  113
<212>  PRT
<213>  Mus musculus

<400>  27

Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr Ala Ala Asn Leu Gln Asp 
1               5                   10                  15      


Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Glu Lys Lys Arg Ala Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly Val Tyr Asn Ala Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Thr Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Thr Leu Ala Pro 
            100                 105                 110         


Arg 
    


<210>  28
<211>  58
<212>  PRT
<213>  Homo sapiens

<400>  28

Glu Ser Pro Lys Ala Gln Ala Ser Ser Val Pro Thr Ala Gln Pro Gln 
1               5                   10                  15      


Ala Glu Gly Ser Leu Ala Lys Ala Thr Thr Ala Pro Ala Thr Thr Arg 
            20                  25                  30          


Asn Thr Gly Arg Gly Gly Glu Glu Lys Lys Lys Glu Lys Glu Lys Glu 
        35                  40                  45              


Glu Gln Glu Glu Arg Glu Thr Lys Thr Pro 
    50                  55              


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially synthesized oligonucleotide primer

<400>  29
aggactccgc cgtgtactat                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially synthesized oligonucleotide primer

<400>  30
tacacgatgt cctgggagct                                                   20


<210>  31
<211>  2217
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially constructed CAR expression cassette


<220>
<221>  CDS
<222>  (1)..(2217)

<400>  31
atg gcc ctg ccc gtg acc gcc ctg ctc ttg ccc ctg gcc ctt ctg ctc         48
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu           
1               5                   10                  15                

cac gcc gcc aga ccc gag gtg cag ctg cag cag agc gga ggc gag aga         96
His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg           
            20                  25                  30                    

gcc acc cct ggc gcc agc gtg aag atg agc tgc aag acc agc ggc tac        144
Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr           
        35                  40                  45                        

acc ttc acc aac tac tgg atg cac tgg gtg aag cag aga ccc ggc cag        192
Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln           
    50                  55                  60                            

ggc ctg gag tgg atc ggc tac atc aac cct agc tcc ggc tac acc cag        240
Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln           
65                  70                  75                  80            

tac aac cag aag ttc aag gac aag gcc acc ctg acc gcc gac aag agc        288
Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser           
                85                  90                  95                

tcc agc acc gcc tac atc cag ctg agc tcc ctg acc agc gag gac tcc        336
Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser           
            100                 105                 110                   

gcc gtg tac tat tgc agc acc tac tac ggc gac tac ctg ttc ccc tac        384
Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr           
        115                 120                 125                       

tgg ggc cag ggc acc ctg gtg acc gtg agc gcc ggc gga ggc gga agc        432
Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser           
    130                 135                 140                           

gga ggc ggc gga tcc gga gga ggc ggc agc gac gtg ctg atg acc cag        480
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln           
145                 150                 155                 160           

acc cct ctg agc ctg ccc gtg agc ctg ggc gac cag gcc agc atc agc        528
Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser           
                165                 170                 175               

tgc aga agc tcc cag gac atc gtg tac ggc aac gga aac acc tac ctg        576
Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu           
            180                 185                 190                   

gag tgg tac ctc cag aag ccc ggc cag agc ccc aag ctg ctg atc tac        624
Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr           
        195                 200                 205                       

aag gtg agc aac aga ttc agc ggc gtg ccc gac aga ttc agc ggc tcc        672
Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser           
    210                 215                 220                           

gga agc gga acc gac ttc acc ctg aag atc agc aga gtg gag gcc gag        720
Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu           
225                 230                 235                 240           

gac ctg ggc gtg tac tat tgc ttc cag ggc agc cac gtg ccc tac acc        768
Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr           
                245                 250                 255               

ttc ggc gga ggc acc aag ctg gag atc aag aga gcg gcc gct atc gag        816
Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu           
            260                 265                 270                   

gtg gag cag aag ctg atc agc gag gag gac ctg cta gac aat gag aag        864
Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys           
        275                 280                 285                       

agc aat gga acc att atc cat gtg aaa ggg aaa cac ctt tgt cca agt        912
Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser           
    290                 295                 300                           

ccc cta ttt ccc gga cct tct aag ccc ttt tgg gtg ctg gtg gtg gtt        960
Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val           
305                 310                 315                 320           

ggt gga gtc ctg gct tgc tat agc ttg cta gta aca gtg gcc ttt att       1008
Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile           
                325                 330                 335               

att ttc tgg gtg agg agt aag agg agc agg ctc ctg cac agt gac tac       1056
Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr           
            340                 345                 350                   

atg aac atg act ccc cgc cgc ccc ggg ccc acc cgc aag cat tac cag       1104
Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln           
        355                 360                 365                       

ccc tat gcc cca cca cgc gac ttc gca gcc tat cgc tcc aga gtg aag       1152
Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys           
    370                 375                 380                           

ttc agc agg agc gca gac gcc ccc gcg tac cag cag ggc cag aac cag       1200
Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln           
385                 390                 395                 400           

ctc tat aac gag ctc aat cta gga cga aga gag gag tac gat gtt ttg       1248
Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu           
                405                 410                 415               

gac aag aga cgt ggc cgg gac cct gag atg ggg gga aag ccg aga agg       1296
Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg           
            420                 425                 430                   

aag aac cct cag gaa ggc ctg tac aat gaa ctg cag aaa gat aag atg       1344
Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met           
        435                 440                 445                       

gcg gag gcc tac agt gag att ggg atg aaa ggc gag cgc cgg agg ggc       1392
Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly           
    450                 455                 460                           

aag ggg cac gat ggc ctt tac cag ggt ctc agt aca gcc acc aag gac       1440
Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp           
465                 470                 475                 480           

acc tac gac gcc ctt cac atg cag gcc ctg ccc cct cgc gcc cga ggc       1488
Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ala Arg Gly           
                485                 490                 495               

gca gca gcg gga gca gga gga gca ggt cga atg gtg agc gag ctg att       1536
Ala Ala Ala Gly Ala Gly Gly Ala Gly Arg Met Val Ser Glu Leu Ile           
            500                 505                 510                   

aag gag aac atg cac atg aag ctg tac atg gag ggc acc gtg aac aac       1584
Lys Glu Asn Met His Met Lys Leu Tyr Met Glu Gly Thr Val Asn Asn           
        515                 520                 525                       

cac cac ttc aag tgc aca tcc gag ggc gaa ggc aag ccc tac gag ggc       1632
His His Phe Lys Cys Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly           
    530                 535                 540                           

acc cag acc atg aga atc aag gcg gtc gag ggc ggc cct ctc ccc ttc       1680
Thr Gln Thr Met Arg Ile Lys Ala Val Glu Gly Gly Pro Leu Pro Phe           
545                 550                 555                 560           

gcc ttc gac atc ctg gct acc agc ttc atg tac ggc agc aaa acc ttc       1728
Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr Gly Ser Lys Thr Phe           
                565                 570                 575               

atc aac cac acc cag ggc atc ccc gac ttc ttt aag cag tcc ttc ccc       1776
Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro           
            580                 585                 590                   

gag ggc ttc aca tgg gag aga gtc acc aca tac gaa gac ggg ggc gtg       1824
Glu Gly Phe Thr Trp Glu Arg Val Thr Thr Tyr Glu Asp Gly Gly Val           
        595                 600                 605                       

ctg acc gct acc cag gac acc agc ctc cag gac ggc tgc ctc atc tac       1872
Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr           
    610                 615                 620                           

aac gtc aag atc aga ggg gtg aac ttc cca tcc aac ggc cct gtg atg       1920
Asn Val Lys Ile Arg Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met           
625                 630                 635                 640           

cag aag aaa aca ctc ggc tgg gag gcc tcc acc gag acc ctg tac ccc       1968
Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr Glu Thr Leu Tyr Pro           
                645                 650                 655               

gct gac ggc ggc ctg gaa ggc aga gcc gac atg gcc ctg aag ctc gtg       2016
Ala Asp Gly Gly Leu Glu Gly Arg Ala Asp Met Ala Leu Lys Leu Val           
            660                 665                 670                   

ggc ggg ggc cac ctg atc tgc aac ttg aag acc aca tac aga tcc aag       2064
Gly Gly Gly His Leu Ile Cys Asn Leu Lys Thr Thr Tyr Arg Ser Lys           
        675                 680                 685                       

aaa ccc gct aag aac ctc aag atg ccc ggc gtc tac tat gtg gac aga       2112
Lys Pro Ala Lys Asn Leu Lys Met Pro Gly Val Tyr Tyr Val Asp Arg           
    690                 695                 700                           

aga ctg gaa aga atc aag gag gcc gac aaa gag acc tac gtc gag cag       2160
Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln           
705                 710                 715                 720           

cac gag gtg gct gtg gcc aga tac tgc gac ctc cct agc aaa ctg ggg       2208
His Glu Val Ala Val Ala Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly           
                725                 730                 735               

cac aga tga                                                           2217
His Arg                                                                   
                                                                          


<210>  32
<211>  738
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  32

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg 
            20                  25                  30          


Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln 
145                 150                 155                 160 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ala Arg Gly 
                485                 490                 495     


Ala Ala Ala Gly Ala Gly Gly Ala Gly Arg Met Val Ser Glu Leu Ile 
            500                 505                 510         


Lys Glu Asn Met His Met Lys Leu Tyr Met Glu Gly Thr Val Asn Asn 
        515                 520                 525             


His His Phe Lys Cys Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly 
    530                 535                 540                 


Thr Gln Thr Met Arg Ile Lys Ala Val Glu Gly Gly Pro Leu Pro Phe 
545                 550                 555                 560 


Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr Gly Ser Lys Thr Phe 
                565                 570                 575     


Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro 
            580                 585                 590         


Glu Gly Phe Thr Trp Glu Arg Val Thr Thr Tyr Glu Asp Gly Gly Val 
        595                 600                 605             


Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr 
    610                 615                 620                 


Asn Val Lys Ile Arg Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met 
625                 630                 635                 640 


Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr Glu Thr Leu Tyr Pro 
                645                 650                 655     


Ala Asp Gly Gly Leu Glu Gly Arg Ala Asp Met Ala Leu Lys Leu Val 
            660                 665                 670         


Gly Gly Gly His Leu Ile Cys Asn Leu Lys Thr Thr Tyr Arg Ser Lys 
        675                 680                 685             


Lys Pro Ala Lys Asn Leu Lys Met Pro Gly Val Tyr Tyr Val Asp Arg 
    690                 695                 700                 


Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln 
705                 710                 715                 720 


His Glu Val Ala Val Ala Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly 
                725                 730                 735     


His Arg 
        


<210>  33
<211>  1668
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially constructed CAR expression cassette


<220>
<221>  CDS
<222>  (1)..(1668)

<400>  33
atg gcc ctg ccc gtg acc gcc ctg ctc ttg ccc ctg gcc ctt ctg ctc         48
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu           
1               5                   10                  15                

cac gcc gcc aga ccc gag gtg cag ctg cag cag agc gga ggc gag aga         96
His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg           
            20                  25                  30                    

gcc acc cct ggc gcc agc gtg aag atg agc tgc aag acc agc ggc tac        144
Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr           
        35                  40                  45                        

acc ttc acc aac tac tgg atg cac tgg gtg aag cag aga ccc ggc cag        192
Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln           
    50                  55                  60                            

ggc ctg gag tgg atc ggc tac atc aac cct agc tcc ggc tac acc cag        240
Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln           
65                  70                  75                  80            

tac aac cag aag ttc aag gac aag gcc acc ctg acc gcc gac aag agc        288
Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser           
                85                  90                  95                

tcc agc acc gcc tac atc cag ctg agc tcc ctg acc agc gag gac tcc        336
Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser           
            100                 105                 110                   

gcc gtg tac tat tgc agc acc tac tac ggc gac tac ctg ttc ccc tac        384
Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr           
        115                 120                 125                       

tgg ggc cag ggc acc ctg gtg acc gtg agc gcc ggc gga ggc gga agc        432
Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser           
    130                 135                 140                           

gga ggc ggc gga tcc gga gga ggc ggc agc gac gtg ctg atg acc cag        480
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln           
145                 150                 155                 160           

acc cct ctg agc ctg ccc gtg agc ctg ggc gac cag gcc agc atc agc        528
Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser           
                165                 170                 175               

tgc aga agc tcc cag gac atc gtg tac ggc aac gga aac acc tac ctg        576
Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu           
            180                 185                 190                   

gag tgg tac ctc cag aag ccc ggc cag agc ccc aag ctg ctg atc tac        624
Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr           
        195                 200                 205                       

aag gtg agc aac aga ttc agc ggc gtg ccc gac aga ttc agc ggc tcc        672
Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser           
    210                 215                 220                           

gga agc gga acc gac ttc acc ctg aag atc agc aga gtg gag gcc gag        720
Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu           
225                 230                 235                 240           

gac ctg ggc gtg tac tat tgc ttc cag ggc agc cac gtg ccc tac acc        768
Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr           
                245                 250                 255               

ttc ggc gga ggc acc aag ctg gag atc aag aga gcg gcc gct atc gag        816
Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu           
            260                 265                 270                   

gtg gag cag aag ctg atc agc gag gag gac ctg cta gac aat gag aag        864
Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys           
        275                 280                 285                       

agc aat gga acc att atc cat gtg aaa ggg aaa cac ctt tgt cca agt        912
Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser           
    290                 295                 300                           

ccc cta ttt ccc gga cct tct aag ccc ttt tgg gtg ctg gtg gtg gtt        960
Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val           
305                 310                 315                 320           

ggt gga gtc ctg gct tgc tat agc ttg cta gta aca gtg gcc ttt att       1008
Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile           
                325                 330                 335               

att ttc tgg gtg agg agt aag agg agc agg ctc ctg cac agt gac tac       1056
Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr           
            340                 345                 350                   

atg aac atg act ccc cgc cgc ccc ggg ccc acc cgc aag cat tac cag       1104
Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln           
        355                 360                 365                       

ccc tat gcc cca cca cgc gac ttc gca gcc tat cgc tcc agg gac cag       1152
Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln           
    370                 375                 380                           

agg ctg ccc ccc gat gcc cac aag ccc cct ggt gag tgc ctc atg gcc       1200
Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Glu Cys Leu Met Ala           
385                 390                 395                 400           

ctg ccg cac tgc tcc tgg cgg gtg agg ccc acc cac caa tct ctc ctt       1248
Leu Pro His Cys Ser Trp Arg Val Arg Pro Thr His Gln Ser Leu Leu           
                405                 410                 415               

ttt tcc tcc cca ggg gga ggc agt ttc cgg acc ccc atc caa gag gag       1296
Phe Ser Ser Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu           
            420                 425                 430                   

cag gcc gac gcc cac tcc acc ctg gcc aag atc aga gtg aag ttc agc       1344
Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser           
        435                 440                 445                       

agg agc gca gac gcc ccc gcg tac cag cag ggc cag aac cag ctc tat       1392
Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr           
    450                 455                 460                           

aac gag ctc aat cta gga cga aga gag gag tac gat gtt ttg gac aag       1440
Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys           
465                 470                 475                 480           

aga cgt ggc cgg gac cct gag atg ggg gga aag ccg aga agg aag aac       1488
Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn           
                485                 490                 495               

cct cag gaa ggc ctg tac aat gaa ctg cag aaa gat aag atg gcg gag       1536
Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu           
            500                 505                 510                   

gcc tac agt gag att ggg atg aaa ggc gag cgc cgg agg ggc aag ggg       1584
Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly           
        515                 520                 525                       

cac gat ggc ctt tac cag ggt ctc agt aca gcc acc aag gac acc tac       1632
His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr           
    530                 535                 540                           

gac gcc ctt cac atg cag gcc ctg ccc cct cgc taa                       1668
Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg                               
545                 550                 555                               


<210>  34
<211>  555
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  34

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg 
            20                  25                  30          


Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln 
145                 150                 155                 160 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln 
    370                 375                 380                 


Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Glu Cys Leu Met Ala 
385                 390                 395                 400 


Leu Pro His Cys Ser Trp Arg Val Arg Pro Thr His Gln Ser Leu Leu 
                405                 410                 415     


Phe Ser Ser Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu 
            420                 425                 430         


Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser 
        435                 440                 445             


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
    450                 455                 460                 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
465                 470                 475                 480 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
                485                 490                 495     


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
            500                 505                 510         


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
        515                 520                 525             


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
    530                 535                 540                 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
545                 550                 555 


<210>  35
<211>  1608
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificially constructed CAR expression cassette


<220>
<221>  CDS
<222>  (1)..(1608)

<400>  35
atg gcc ctg ccc gtg acc gcc ctg ctc ttg ccc ctg gcc ctt ctg ctc         48
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu           
1               5                   10                  15                

cac gcc gcc aga ccc gag gtg cag ctg cag cag agc gga ggc gag aga         96
His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg           
            20                  25                  30                    

gcc acc cct ggc gcc agc gtg aag atg agc tgc aag acc agc ggc tac        144
Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr           
        35                  40                  45                        

acc ttc acc aac tac tgg atg cac tgg gtg aag cag aga ccc ggc cag        192
Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln           
    50                  55                  60                            

ggc ctg gag tgg atc ggc tac atc aac cct agc tcc ggc tac acc cag        240
Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln           
65                  70                  75                  80            

tac aac cag aag ttc aag gac aag gcc acc ctg acc gcc gac aag agc        288
Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser           
                85                  90                  95                

tcc agc acc gcc tac atc cag ctg agc tcc ctg acc agc gag gac tcc        336
Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser           
            100                 105                 110                   

gcc gtg tac tat tgc agc acc tac tac ggc gac tac ctg ttc ccc tac        384
Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr           
        115                 120                 125                       

tgg ggc cag ggc acc ctg gtg acc gtg agc gcc ggc gga ggc gga agc        432
Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser           
    130                 135                 140                           

gga ggc ggc gga tcc gga gga ggc ggc agc gac gtg ctg atg acc cag        480
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln           
145                 150                 155                 160           

acc cct ctg agc ctg ccc gtg agc ctg ggc gac cag gcc agc atc agc        528
Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser           
                165                 170                 175               

tgc aga agc tcc cag gac atc gtg tac ggc aac gga aac acc tac ctg        576
Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu           
            180                 185                 190                   

gag tgg tac ctc cag aag ccc ggc cag agc ccc aag ctg ctg atc tac        624
Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr           
        195                 200                 205                       

aag gtg agc aac aga ttc agc ggc gtg ccc gac aga ttc agc ggc tcc        672
Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser           
    210                 215                 220                           

gga agc gga acc gac ttc acc ctg aag atc agc aga gtg gag gcc gag        720
Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu           
225                 230                 235                 240           

gac ctg ggc gtg tac tat tgc ttc cag ggc agc cac gtg ccc tac acc        768
Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr           
                245                 250                 255               

ttc ggc gga ggc acc aag ctg gag atc aag aga gcg gcc gct atc gag        816
Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu           
            260                 265                 270                   

gtg gag cag aag ctg atc agc gag gag gac ctg cta gac aat gag aag        864
Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys           
        275                 280                 285                       

agc aat gga acc att atc cat gtg aaa ggg aaa cac ctt tgt cca agt        912
Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser           
    290                 295                 300                           

ccc cta ttt ccc gga cct tct aag ccc ttt tgg gtg ctg gtg gtg gtt        960
Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val           
305                 310                 315                 320           

ggt gga gtc ctg gct tgc tat agc ttg cta gta aca gtg gcc ttt att       1008
Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile           
                325                 330                 335               

att ttc tgg gtg agg agt aag agg agc agg ctc ctg cac agt gac tac       1056
Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr           
            340                 345                 350                   

atg aac atg act ccc cgc cgc ccc ggg ccc acc cgc aag cat tac cag       1104
Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln           
        355                 360                 365                       

ccc tat gcc cca cca cgc gac ttc gca gcc tat cgc tcc aaa cgg ggc       1152
Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly           
    370                 375                 380                           

aga aag aaa ctc ctg tat ata ttc aaa caa cca ttt atg aga cca gta       1200
Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val           
385                 390                 395                 400           

caa act act caa gag gaa gat ggc tgt agc tgc cga ttt cca gaa gaa       1248
Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu           
                405                 410                 415               

gaa gaa gga gga tgt gaa ctg aga gtg aag ttc agc agg agc gca gac       1296
Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp           
            420                 425                 430                   

gcc ccc gcg tac cag cag ggc cag aac cag ctc tat aac gag ctc aat       1344
Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn           
        435                 440                 445                       

cta gga cga aga gag gag tac gat gtt ttg gac aag aga cgt ggc cgg       1392
Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg           
    450                 455                 460                           

gac cct gag atg ggg gga aag ccg aga agg aag aac cct cag gaa ggc       1440
Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly           
465                 470                 475                 480           

ctg tac aat gaa ctg cag aaa gat aag atg gcg gag gcc tac agt gag       1488
Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu           
                485                 490                 495               

att ggg atg aaa ggc gag cgc cgg agg ggc aag ggg cac gat ggc ctt       1536
Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu           
            500                 505                 510                   

tac cag ggt ctc agt aca gcc acc aag gac acc tac gac gcc ctt cac       1584
Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His           
        515                 520                 525                       

atg cag gcc ctg ccc cct cgc taa                                       1608
Met Gln Ala Leu Pro Pro Arg                                               
    530                 535                                               


<210>  36
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  36

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Gln Gln Ser Gly Gly Glu Arg 
            20                  25                  30          


Ala Thr Pro Gly Ala Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Lys Gln Arg Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser 
                85                  90                  95      


Ser Ser Thr Ala Tyr Ile Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln 
145                 150                 155                 160 


Thr Pro Leu Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly 
    370                 375                 380                 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
385                 390                 395                 400 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
                405                 410                 415     


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
            420                 425                 430         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        435                 440                 445             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    450                 455                 460                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
465                 470                 475                 480 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                485                 490                 495     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            500                 505                 510         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        515                 520                 525             


Met Gln Ala Leu Pro Pro Arg 
    530                 535 


<210>  37
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial humanized heavy chain coding sequence

<400>  37
atggacccca agggcagcct gagctggaga atcctgctgt tcctgagcct ggccttcgag       60

ctgagctacg gccaggtgca gctggtgcag agcggagccg aagtgaagaa gcccggagcc      120

agcgtgaagg tgagctgcaa ggcgtctggc tacaccttta ccaactactg gatgcactgg      180

gtgaggcagg caccaggcca aggcctggag tggatgggct acatcaaccc cagcagcggt      240

tacacgcagt acaatcagaa gttccagggc agggtgacca tgaccaggga cacaagtacc      300

agcaccgttt acatggagct gagcagcctg aggagcgagg acaccgccgt gtactactgc      360

agcacctact acggggacta cctgttcccc tactggggcc agggaaccct ggttaccgtg      420

agctctgcta gcaccaaggg cccatcggtc ttccccctgg cgccctgctc caggagcacc      480

tccgagagca cagcggccct gggctgcctg gtcaaggact acttccccga accggtgacg      540

gtgtcgtgga actcaggcgc tctgaccagc ggcgtgcaca ccttcccggc tgtcctacag      600

tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcaa cttcggcacc      660

cagacctaca cctgcaacgt agatcacaag cccagcaaca ccaaggtgga caagacagtt      720

gagcgcaaat gttgtgtcga gtgcccaccg tgcccagcac cacctgtggc aggaccgtca      780

gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc      840

acgtgcgtgg tggtggacgt gagccacgaa gaccccgagg tccagttcaa ctggtacgtg      900

gacggcgtgg aggtgcataa tgccaagaca aagccacggg aggagcagtt caacagcacg      960

ttccgtgtgg tcagcgtcct caccgttgtg caccaggact ggctgaacgg caaggagtac     1020

aagtgcaagg tctccaacaa aggcctccca gcccccatcg agaaaaccat ctccaaaacc     1080

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1140

aagaaccagg tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg     1200

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacacctcc catgctggac     1260

tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag     1320

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1380

agcctctccc tgtctccggg ttagtaa                                         1407


<210>  38
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial humanized heavy chain coding sequence

<400>  38
atggacccca agggcagcct gagctggaga atcctgctgt tcctgagcct ggccttcgag       60

ctgagctacg gccaggtgca gctggtgcag agcggagccg aagtgaagaa gcccggagcc      120

agcgtgaagg tgagctgcaa ggcgtctggc tacaccttta ccaactactg gatgcactgg      180

gtgaggcagg caccaggcca aggcctggag tggatgggct acatcaaccc cagcagcggt      240

tacacgcagt acaatcagaa gttccagggc agggtgacca tgaccgcaga cacaagtacc      300

agcaccgttt acatggagct gagcagcctg aggagcgagg acaccgccgt gtactactgc      360

agcacctact acggggacta cctgttcccc tactggggcc agggaaccct ggttaccgtg      420

agctctgcta gcaccaaggg cccatcggtc ttccccctgg cgccctgctc caggagcacc      480

tccgagagca cagcggccct gggctgcctg gtcaaggact acttccccga accggtgacg      540

gtgtcgtgga actcaggcgc tctgaccagc ggcgtgcaca ccttcccggc tgtcctacag      600

tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcaa cttcggcacc      660

cagacctaca cctgcaacgt agatcacaag cccagcaaca ccaaggtgga caagacagtt      720

gagcgcaaat gttgtgtcga gtgcccaccg tgcccagcac cacctgtggc aggaccgtca      780

gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc      840

acgtgcgtgg tggtggacgt gagccacgaa gaccccgagg tccagttcaa ctggtacgtg      900

gacggcgtgg aggtgcataa tgccaagaca aagccacggg aggagcagtt caacagcacg      960

ttccgtgtgg tcagcgtcct caccgttgtg caccaggact ggctgaacgg caaggagtac     1020

aagtgcaagg tctccaacaa aggcctccca gcccccatcg agaaaaccat ctccaaaacc     1080

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1140

aagaaccagg tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg     1200

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacacctcc catgctggac     1260

tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag     1320

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1380

agcctctccc tgtctccggg ttagtaa                                         1407


<210>  39
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial humanized heavy chain coding sequence

<400>  39
atggacccca agggcagcct gagctggaga atcctgctgt tcctgagcct ggccttcgag       60

ctgagctacg gcgaggtgca gctggtgcag agcggagccg aagtgaagaa gcccggagcc      120

agcgtgaagg tgagctgcaa ggcgtctggc tacaccttta ccaactactg gatgcactgg      180

gtgaggcagg caccaggcca aggcctggag tggatgggct acatcaaccc cagcagcggt      240

tacacgcagt acaatcagaa gttccagggc tgggtgacca tgaccgcaga cacaagtatc      300

agcaccgctt acatggagct gagccgcctg aggagcgatg acaccgccgt gtactactgc      360

agcacctact acggggacta cctgttcccc tactggggcc agggaaccct ggttaccgtg      420

agctctgcta gcaccaaggg cccatcggtc ttccccctgg cgccctgctc caggagcacc      480

tccgagagca cagcggccct gggctgcctg gtcaaggact acttccccga accggtgacg      540

gtgtcgtgga actcaggcgc tctgaccagc ggcgtgcaca ccttcccggc tgtcctacag      600

tcctcaggac tctactccct cagcagcgtg gtgaccgtgc cctccagcaa cttcggcacc      660

cagacctaca cctgcaacgt agatcacaag cccagcaaca ccaaggtgga caagacagtt      720

gagcgcaaat gttgtgtcga gtgcccaccg tgcccagcac cacctgtggc aggaccgtca      780

gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc      840

acgtgcgtgg tggtggacgt gagccacgaa gaccccgagg tccagttcaa ctggtacgtg      900

gacggcgtgg aggtgcataa tgccaagaca aagccacggg aggagcagtt caacagcacg      960

ttccgtgtgg tcagcgtcct caccgttgtg caccaggact ggctgaacgg caaggagtac     1020

aagtgcaagg tctccaacaa aggcctccca gcccccatcg agaaaaccat ctccaaaacc     1080

aaagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc     1140

aagaaccagg tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg     1200

gagtgggaga gcaatgggca gccggagaac aactacaaga ccacacctcc catgctggac     1260

tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag     1320

gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag     1380

agcctctccc tgtctccggg ttagtaa                                         1407


<210>  40
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial humanized light chain coding sequence

<400>  40
atggagaccg acaccctgct gctctgggtg ctgctgctct gggtgcccgg ctccaccgga       60

gacgtggtga tgacccagag ccccctgagc ctgcccgtga ccctgggcca gcccgccagc      120

atcagctgca ggagcagcca ggacatcgtg tatggcaacg gcaacaccta cctggagtgg      180

ttccagcaga ggccaggcca gagtcccagg ctgttgatct acaaggtgag caataggttc      240

agcggcgtgc ccgacaggtt ctctggctcc ggcagcggca ccgacttcac cctgaagatc      300

agcagggtgg aagccgagga cgtgggcgtg tactactgct tccagggctc tcacgtgccc      360

tacaccttcg gccagggcac taagctggag atcaagcgga ccgtggccgc ccccagcgtg      420

ttcatcttcc ctcccagcga cgagcagctg aagtctggca ccgccagcgt ggtgtgcctg      480

ctgaacaact tctacccccg cgaggccaag gtgcagtgga aggtggacaa cgccctgcag      540

agcggcaaca gccaggagag cgtgaccgag caggactcca aggacagcac ctacagcctg      600

agcagcaccc tgaccctgag caaggccgac tacgagaagc acaaggtgta cgcctgcgag      660

gtgacccacc agggactgtc tagccccgtg accaagagct tcaaccgggg cgagtgctaa      720


<210>  41
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial humanized light chain coding sequence

<400>  41
atggagaccg acaccctgct gctctgggtg ctgctgctct gggtgcccgg ctccaccgga       60

gacgtggtga tgacccagag ccccctgagc ctgcccgtga ccctgggcca gcccgccagc      120

atcagctgca ggagcagcca ggacatcgtg tatggcaacg gcaacaccta cctggagtgg      180

tatcagcaga ggccaggcca gagtcccagg ctgttgatct acaaggtgag caataggttc      240

agcggcgtgc ccgacaggtt ctctggctcc ggcagcggca ccgacttcac cctgaagatc      300

agcagggtgg aagccgagga cgtgggcgtg tactactgct tccagggctc tcacgtgccc      360

tacaccttcg gccagggcac taagctggag atcaagcgga ccgtggccgc ccccagcgtg      420

ttcatcttcc ctcccagcga cgagcagctg aagtctggca ccgccagcgt ggtgtgcctg      480

ctgaacaact tctacccccg cgaggccaag gtgcagtgga aggtggacaa cgccctgcag      540

agcggcaaca gccaggagag cgtgaccgag caggactcca aggacagcac ctacagcctg      600

agcagcaccc tgaccctgag caaggccgac tacgagaagc acaaggtgta cgcctgcgag      660

gtgacccacc agggactgtc tagccccgtg accaagagct tcaaccgggg cgagtgctaa      720


<210>  42
<211>  720
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Artificial humanized light chain coding sequence

<400>  42
atggagaccg acaccctgct gctctgggtg ctgctgctct gggtgcccgg ctccaccgga       60

gacatcgtgc tgacccagag ccccctgagc ctgcccgtga ccctgggcca gcccgccagc      120

atcagctgca ggagcagcca ggacatcgtg tatggcaacg gcaacaccta cctggagtgg      180

tatcagcaga ggccaggcca gagtcccagg ctgttgatct acaaggtgag caataggttc      240

agcggcgtgc ccgacagctt ctctggctcc ggcagcggca ccgacttcac cctgaccatc      300

agcagggtgg aagccgagga cgtgggcatc tactactgct tccagggctc tcacgtgccc      360

tacaccttcg gcgagggcac taagctggag atcaagcgga ccgtggccgc ccccagcgtg      420

ttcatcttcc ctcccagcga cgagcagctg aagtctggca ccgccagcgt ggtgtgcctg      480

ctgaacaact tctacccccg cgaggccaag gtgcagtgga aggtggacaa cgccctgcag      540

agcggcaaca gccaggagag cgtgaccgag caggactcca aggacagcac ctacagcctg      600

agcagcaccc tgaccctgag caaggccgac tacgagaagc acaaggtgta cgcctgcgag      660

gtgacccacc agggactgtc tagccccgtg accaagagct tcaaccgggg cgagtgctaa      720


<210>  43
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial humanized heavy chain amino acid sequence

<400>  43

Met Asp Pro Lys Gly Ser Leu Ser Trp Arg Ile Leu Leu Phe Leu Ser 
1               5                   10                  15      


Leu Ala Phe Glu Leu Ser Tyr Gly Gln Val Gln Leu Val Gln Ser Gly 
            20                  25                  30          


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
        35                  40                  45              


Ser Gly Tyr Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala 
    50                  55                  60                  


Pro Gly Gln Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly 
65                  70                  75                  80  


Tyr Thr Gln Tyr Asn Gln Lys Phe Gln Gly Arg Val Thr Met Thr Arg 
                85                  90                  95      


Asp Thr Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser 
            100                 105                 110         


Glu Asp Thr Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu 
        115                 120                 125             


Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr 
145                 150                 155                 160 


Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                165                 170                 175     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            180                 185                 190         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        195                 200                 205             


Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr Tyr Thr 
    210                 215                 220                 


Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Thr Val 
225                 230                 235                 240 


Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
                245                 250                 255     


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
305                 310                 315                 320 


Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
        355                 360                 365             


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
    370                 375                 380                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
385                 390                 395                 400 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                405                 410                 415     


Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            420                 425                 430         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        435                 440                 445             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    450                 455                 460                 


Ser Pro Gly 
465         


<210>  44
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial humanized heavy chain amino acid sequence

<400>  44

Met Asp Pro Lys Gly Ser Leu Ser Trp Arg Ile Leu Leu Phe Leu Ser 
1               5                   10                  15      


Leu Ala Phe Glu Leu Ser Tyr Gly Gln Val Gln Leu Val Gln Ser Gly 
            20                  25                  30          


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
        35                  40                  45              


Ser Gly Tyr Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala 
    50                  55                  60                  


Pro Gly Gln Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly 
65                  70                  75                  80  


Tyr Thr Gln Tyr Asn Gln Lys Phe Gln Gly Arg Val Thr Met Thr Ala 
                85                  90                  95      


Asp Thr Ser Thr Ser Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser 
            100                 105                 110         


Glu Asp Thr Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu 
        115                 120                 125             


Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr 
145                 150                 155                 160 


Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                165                 170                 175     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            180                 185                 190         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        195                 200                 205             


Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr Tyr Thr 
    210                 215                 220                 


Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Thr Val 
225                 230                 235                 240 


Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
                245                 250                 255     


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
305                 310                 315                 320 


Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
        355                 360                 365             


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
    370                 375                 380                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
385                 390                 395                 400 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                405                 410                 415     


Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            420                 425                 430         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        435                 440                 445             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    450                 455                 460                 


Ser Pro Gly 
465         


<210>  45
<211>  467
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial humanized heavy chain amino acid sequence

<400>  45

Met Asp Pro Lys Gly Ser Leu Ser Trp Arg Ile Leu Leu Phe Leu Ser 
1               5                   10                  15      


Leu Ala Phe Glu Leu Ser Tyr Gly Glu Val Gln Leu Val Gln Ser Gly 
            20                  25                  30          


Ala Glu Val Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala 
        35                  40                  45              


Ser Gly Tyr Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala 
    50                  55                  60                  


Pro Gly Gln Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly 
65                  70                  75                  80  


Tyr Thr Gln Tyr Asn Gln Lys Phe Gln Gly Trp Val Thr Met Thr Ala 
                85                  90                  95      


Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser 
            100                 105                 110         


Asp Asp Thr Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu 
        115                 120                 125             


Phe Pro Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser 
    130                 135                 140                 


Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr 
145                 150                 155                 160 


Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro 
                165                 170                 175     


Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val 
            180                 185                 190         


His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser 
        195                 200                 205             


Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr Tyr Thr 
    210                 215                 220                 


Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys Thr Val 
225                 230                 235                 240 


Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
                245                 250                 255     


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            260                 265                 270         


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        275                 280                 285             


His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
    290                 295                 300                 


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
305                 310                 315                 320 


Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
                325                 330                 335     


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
            340                 345                 350         


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
        355                 360                 365             


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
    370                 375                 380                 


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
385                 390                 395                 400 


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                405                 410                 415     


Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
            420                 425                 430         


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
        435                 440                 445             


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    450                 455                 460                 


Ser Pro Gly 
465         


<210>  46
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial humanized light chain amino acid sequence

<400>  46

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro 
            20                  25                  30          


Val Thr Leu Gly Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Asp 
        35                  40                  45              


Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu Glu Trp Phe Gln Gln Arg 
    50                  55                  60                  


Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr 
            100                 105                 110         


Cys Phe Gln Gly Ser His Val Pro Tyr Thr Phe Gly Gln Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro 
    130                 135                 140                 


Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu 
145                 150                 155                 160 


Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp 
                165                 170                 175     


Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp 
            180                 185                 190         


Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys 
        195                 200                 205             


Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln 
    210                 215                 220                 


Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235                 


<210>  47
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial humanized light chain amino acid sequence

<400>  47

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro 
            20                  25                  30          


Val Thr Leu Gly Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Asp 
        35                  40                  45              


Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Gln Gln Arg 
    50                  55                  60                  


Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe 
65                  70                  75                  80  


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr 
            100                 105                 110         


Cys Phe Gln Gly Ser His Val Pro Tyr Thr Phe Gly Gln Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro 
    130                 135                 140                 


Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu 
145                 150                 155                 160 


Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp 
                165                 170                 175     


Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp 
            180                 185                 190         


Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys 
        195                 200                 205             


Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln 
    210                 215                 220                 


Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235                 


<210>  48
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial humanized light chain amino acid sequence

<400>  48

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Ile Val Leu Thr Gln Ser Pro Leu Ser Leu Pro 
            20                  25                  30          


Val Thr Leu Gly Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Asp 
        35                  40                  45              


Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Gln Gln Arg 
    50                  55                  60                  


Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe 
65                  70                  75                  80  


Ser Gly Val Pro Asp Ser Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
                85                  90                  95      


Thr Leu Thr Ile Ser Arg Val Glu Ala Glu Asp Val Gly Ile Tyr Tyr 
            100                 105                 110         


Cys Phe Gln Gly Ser His Val Pro Tyr Thr Phe Gly Glu Gly Thr Lys 
        115                 120                 125             


Leu Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro 
    130                 135                 140                 


Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu 
145                 150                 155                 160 


Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp 
                165                 170                 175     


Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp 
            180                 185                 190         


Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys 
        195                 200                 205             


Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln 
    210                 215                 220                 


Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235                 


<210>  49
<211>  738
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially constructed 2nd GEN CAR expression cassette

<400>  49

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Gln Gly Trp Val Thr Met Thr Ala Asp Thr Ser 
                85                  90                  95      


Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
145                 150                 155                 160 


Ser Pro Leu Ser Leu Pro Val Thr Leu Gly Gln Pro Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ala Arg Gly 
                485                 490                 495     


Ala Ala Ala Gly Ala Gly Gly Ala Gly Arg Met Val Ser Glu Leu Ile 
            500                 505                 510         


Lys Glu Asn Met His Met Lys Leu Tyr Met Glu Gly Thr Val Asn Asn 
        515                 520                 525             


His His Phe Lys Cys Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly 
    530                 535                 540                 


Thr Gln Thr Met Arg Ile Lys Ala Val Glu Gly Gly Pro Leu Pro Phe 
545                 550                 555                 560 


Ala Phe Asp Ile Leu Ala Thr Ser Phe Met Tyr Gly Ser Lys Thr Phe 
                565                 570                 575     


Ile Asn His Thr Gln Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro 
            580                 585                 590         


Glu Gly Phe Thr Trp Glu Arg Val Thr Thr Tyr Glu Asp Gly Gly Val 
        595                 600                 605             


Leu Thr Ala Thr Gln Asp Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr 
    610                 615                 620                 


Asn Val Lys Ile Arg Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met 
625                 630                 635                 640 


Gln Lys Lys Thr Leu Gly Trp Glu Ala Ser Thr Glu Thr Leu Tyr Pro 
                645                 650                 655     


Ala Asp Gly Gly Leu Glu Gly Arg Ala Asp Met Ala Leu Lys Leu Val 
            660                 665                 670         


Gly Gly Gly His Leu Ile Cys Asn Leu Lys Thr Thr Tyr Arg Ser Lys 
        675                 680                 685             


Lys Pro Ala Lys Asn Leu Lys Met Pro Gly Val Tyr Tyr Val Asp Arg 
    690                 695                 700                 


Arg Leu Glu Arg Ile Lys Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln 
705                 710                 715                 720 


His Glu Val Ala Val Ala Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly 
                725                 730                 735     


His Arg 
        


<210>  50
<211>  555
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially constructed 3rd GEN CAR expression cassette

<400>  50

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Gln Gly Trp Val Thr Met Thr Ala Asp Thr Ser 
                85                  90                  95      


Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
145                 150                 155                 160 


Ser Pro Leu Ser Leu Pro Val Thr Leu Gly Gln Pro Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln 
    370                 375                 380                 


Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Glu Cys Leu Met Ala 
385                 390                 395                 400 


Leu Pro His Cys Ser Trp Arg Val Arg Pro Thr His Gln Ser Leu Leu 
                405                 410                 415     


Phe Ser Ser Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu 
            420                 425                 430         


Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser 
        435                 440                 445             


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
    450                 455                 460                 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
465                 470                 475                 480 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
                485                 490                 495     


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
            500                 505                 510         


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
        515                 520                 525             


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
    530                 535                 540                 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
545                 550                 555 


<210>  51
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially constructed 3rd GEN CAR expression cassette

<400>  51

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Gln Gly Trp Val Thr Met Thr Ala Asp Thr Ser 
                85                  90                  95      


Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
145                 150                 155                 160 


Ser Pro Leu Ser Leu Pro Val Thr Leu Gly Gln Pro Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly 
    370                 375                 380                 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
385                 390                 395                 400 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
                405                 410                 415     


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
            420                 425                 430         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        435                 440                 445             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    450                 455                 460                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
465                 470                 475                 480 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                485                 490                 495     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            500                 505                 510         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        515                 520                 525             


Met Gln Ala Leu Pro Pro Arg 
    530                 535 


<210>  52
<211>  800
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially constructed and labeled 3rd GEN CAR expression 
       cassette

<400>  52

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Gln Gly Trp Val Thr Met Thr Ala Asp Thr Ser 
                85                  90                  95      


Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
145                 150                 155                 160 


Ser Pro Leu Ser Leu Pro Val Thr Leu Gly Gln Pro Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln 
    370                 375                 380                 


Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Glu Cys Leu Met Ala 
385                 390                 395                 400 


Leu Pro His Cys Ser Trp Arg Val Arg Pro Thr His Gln Ser Leu Leu 
                405                 410                 415     


Phe Ser Ser Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu 
            420                 425                 430         


Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe Ser 
        435                 440                 445             


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
    450                 455                 460                 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
465                 470                 475                 480 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
                485                 490                 495     


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
            500                 505                 510         


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
        515                 520                 525             


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
    530                 535                 540                 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Ala Arg Gly Ala Ala 
545                 550                 555                 560 


Ala Gly Ala Gly Gly Ala Gly Arg Met Val Ser Glu Leu Ile Lys Glu 
                565                 570                 575     


Asn Met His Met Lys Leu Tyr Met Glu Gly Thr Val Asn Asn His His 
            580                 585                 590         


Phe Lys Cys Thr Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly Thr Gln 
        595                 600                 605             


Thr Met Arg Ile Lys Ala Val Glu Gly Gly Pro Leu Pro Phe Ala Phe 
    610                 615                 620                 


Asp Ile Leu Ala Thr Ser Phe Met Tyr Gly Ser Lys Thr Phe Ile Asn 
625                 630                 635                 640 


His Thr Gln Gly Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly 
                645                 650                 655     


Phe Thr Trp Glu Arg Val Thr Thr Tyr Glu Asp Gly Gly Val Leu Thr 
            660                 665                 670         


Ala Thr Gln Asp Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr Asn Val 
        675                 680                 685             


Lys Ile Arg Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys 
    690                 695                 700                 


Lys Thr Leu Gly Trp Glu Ala Ser Thr Glu Thr Leu Tyr Pro Ala Asp 
705                 710                 715                 720 


Gly Gly Leu Glu Gly Arg Ala Asp Met Ala Leu Lys Leu Val Gly Gly 
                725                 730                 735     


Gly His Leu Ile Cys Asn Leu Lys Thr Thr Tyr Arg Ser Lys Lys Pro 
            740                 745                 750         


Ala Lys Asn Leu Lys Met Pro Gly Val Tyr Tyr Val Asp Arg Arg Leu 
        755                 760                 765             


Glu Arg Ile Lys Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln His Glu 
    770                 775                 780                 


Val Ala Val Ala Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly His Arg 
785                 790                 795                 800 


<210>  53
<211>  780
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificially constructed and labeled 3rd GEN CAR expression 
       cassette

<400>  53

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val 
            20                  25                  30          


Lys Lys Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr 
        35                  40                  45              


Thr Phe Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala Pro Gly Gln 
    50                  55                  60                  


Gly Leu Glu Trp Met Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Gln 
65                  70                  75                  80  


Tyr Asn Gln Lys Phe Gln Gly Trp Val Thr Met Thr Ala Asp Thr Ser 
                85                  90                  95      


Ile Ser Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys Ser Thr Tyr Tyr Gly Asp Tyr Leu Phe Pro Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
145                 150                 155                 160 


Ser Pro Leu Ser Leu Pro Val Thr Leu Gly Gln Pro Ala Ser Ile Ser 
                165                 170                 175     


Cys Arg Ser Ser Gln Asp Ile Val Tyr Gly Asn Gly Asn Thr Tyr Leu 
            180                 185                 190         


Glu Trp Tyr Gln Gln Arg Pro Gly Gln Ser Pro Arg Leu Leu Ile Tyr 
        195                 200                 205             


Lys Val Ser Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 


Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu 
225                 230                 235                 240 


Asp Val Gly Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Tyr Thr 
                245                 250                 255     


Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Ala Ala Ala Ile Glu 
            260                 265                 270         


Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys 
        275                 280                 285             


Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser 
    290                 295                 300                 


Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val 
305                 310                 315                 320 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
                325                 330                 335     


Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
            340                 345                 350         


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
        355                 360                 365             


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly 
    370                 375                 380                 


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
385                 390                 395                 400 


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
                405                 410                 415     


Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp 
            420                 425                 430         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        435                 440                 445             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    450                 455                 460                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
465                 470                 475                 480 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                485                 490                 495     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            500                 505                 510         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        515                 520                 525             


Met Gln Ala Leu Pro Pro Arg Ala Arg Gly Ala Ala Ala Gly Ala Gly 
    530                 535                 540                 


Gly Ala Gly Arg Met Val Ser Glu Leu Ile Lys Glu Asn Met His Met 
545                 550                 555                 560 


Lys Leu Tyr Met Glu Gly Thr Val Asn Asn His His Phe Lys Cys Thr 
                565                 570                 575     


Ser Glu Gly Glu Gly Lys Pro Tyr Glu Gly Thr Gln Thr Met Arg Ile 
            580                 585                 590         


Lys Ala Val Glu Gly Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala 
        595                 600                 605             


Thr Ser Phe Met Tyr Gly Ser Lys Thr Phe Ile Asn His Thr Gln Gly 
    610                 615                 620                 


Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly Phe Thr Trp Glu 
625                 630                 635                 640 


Arg Val Thr Thr Tyr Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp 
                645                 650                 655     


Thr Ser Leu Gln Asp Gly Cys Leu Ile Tyr Asn Val Lys Ile Arg Gly 
            660                 665                 670         


Val Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly 
        675                 680                 685             


Trp Glu Ala Ser Thr Glu Thr Leu Tyr Pro Ala Asp Gly Gly Leu Glu 
    690                 695                 700                 


Gly Arg Ala Asp Met Ala Leu Lys Leu Val Gly Gly Gly His Leu Ile 
705                 710                 715                 720 


Cys Asn Leu Lys Thr Thr Tyr Arg Ser Lys Lys Pro Ala Lys Asn Leu 
                725                 730                 735     


Lys Met Pro Gly Val Tyr Tyr Val Asp Arg Arg Leu Glu Arg Ile Lys 
            740                 745                 750         


Glu Ala Asp Lys Glu Thr Tyr Val Glu Gln His Glu Val Ala Val Ala 
        755                 760                 765             


Arg Tyr Cys Asp Leu Pro Ser Lys Leu Gly His Arg 
    770                 775                 780 


