                         SEQUENCE LISTING

<110>  National University Of Singapore
 
<120>  T CELL MODIFIED WITH A SYNTHETIC RECEPTOR CONTAINING A SINGLE 
       ITAM SIGNALING MOTIF

<130>  10104SG1295

<150>  10201908256R
<151>  2019-09-06

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  4853
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of the pZTS4-Ef1a-NKG2D-IgG4H-CD28TM-DAP12 construct

<400>  1
ggtctcctcc caataacgat aatagatacg gaacggacac gcagctagat taaccctaga       60

aagataatca tattgtgacg tacgttaaag ataatcatgt gtaaaattga cgcatgaaaa      120

cttttatgag ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg      180

ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc      240

ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc      300

ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaag      360

gcgggatcca ctgaggggta ccgctccggt gcccgtcagt gggcagagcg cacatcgccc      420

acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg      480

cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg      540

ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc      600

gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat      660

ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag      720

cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct      780

cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac      840

cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct      900

gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact      960

ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt     1020

tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct     1080

ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca     1140

aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct     1200

gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca     1260

caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg     1320

gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg     1380

ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg     1440

ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg     1500

ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga     1560

aagcttagct gaattctaat acgactcact atagggaaat aagagagaaa agaagagtaa     1620

gaagaaatat aagagccacc atgcttctcc tggtgacaag ccttctgctc tgtgagttac     1680

cacacccagc attcctcctg atcccaggcg cgcatgcctt caaccaagaa gttcaaattc     1740

ccttgaccga aagttactgt ggcccatgtc ctaaaaactg gatatgttac aaaaataact     1800

gctaccaatt ttttgatgag agtaaaaact ggtatgagag ccaggcttct tgtatgtctc     1860

aaaatgccag ccttctgaaa gtatacagca aagaggacca ggatttactt aaactggtga     1920

agtcatatca ttggatggga ctagtacaca ttccaacaaa tggatcttgg cagtgggaag     1980

atggctccat tctctcaccc aacctactaa caataattga aatgcagaag ggagactgtg     2040

cactctatgc ctcgagcttt aaaggctata tagaaaactg ttcaactcca aatacgtaca     2100

tctgtatgca aaggactgtg gctagcaact ggtcacaccc ccagtttgag aaaggtgggg     2160

gagggtcagg tggtgggggg tcaaattggt ctcatcccca gttcgagaag ggcggaggag     2220

gttcaggtgg gggtgggagc aactggagcc accctcaatt tgaaaaagga ggtggaggga     2280

gtggaggtgg tggatctgaa tctaaatatg gccccccttg cccaccatgt cccttctggg     2340

ttttggtagt tgtcggcggg gtgcttgctt gctattcatt gttggttacg gtggctttta     2400

taattttctg ggtaagattc tctgtggtga agaggggtag aaagaagctc ctctatatct     2460

ttaagcaacc ttttatgcgg ccagtacaga ctactcaaga ggaggacgga tgttcatgcc     2520

gattccccga agaggaagaa ggggggtgcg agttgtactt cctgggacga cttgttcctc     2580

gggggcgggg tgctgcagaa gctgcaacgc gaaaacaaag aattaccgag actgagagtc     2640

cgtatcaaga actgcagggc caaagaagcg acgtttactc cgatctcaat acccaacggc     2700

cttactacaa atgagtcgac tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa     2760

tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat     2820

aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg     2880

gaggtttttt aaagcaagta aaacctctac aaatgtggta cttaagaatg catgcttttt     2940

ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag     3000

cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg     3060

cggggggagc gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg     3120

gagggacgta attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca     3180

tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcagtg tatgaaatgc     3240

tttaaggagg agagggggag accaaagggc gagacgttaa ggcctcacgt gacatgtgag     3300

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     3360

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     3420

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     3480

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     3540

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     3600

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     3660

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     3720

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     3780

gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     3840

aaagagttgg tagctcttga tccggcaaac aaaccacgct ggtagcggtg gtttttttgt     3900

ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc     3960

tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgccgtc     4020

tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat     4080

accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg     4140

ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa     4200

tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac     4260

gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc     4320

gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt     4380

acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag     4440

cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg     4500

agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc     4560

agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg     4620

cgctgcctcg tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac     4680

cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg     4740

tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc     4800

atcttgttca atcataatat tattgaagca tttatcaggg ttcgtctcgt ccc            4853


<210>  2
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of GMCSFR-alpha (UniProtKB - P15509) signal peptide 
       (aa 1-22)

<400>  2
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atccca                                                                  66


<210>  3
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of GMCSFR-alpha (UniProtKB - P15509) signal 
       peptide (aa 1-22)

<400>  3

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro 
            20          


<210>  4
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of GMCSFR-alpha signal peptide with deduced amino 
       acid

<400>  4
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg       60

atcca                                                                   65


<210>  5
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the NKG2D extra-cellular domain

<400>  5
ttcaaccaag aagttcaaat tcccttgacc gaaagttact gtggcccatg tcctaaaaac       60

tggatatgtt acaaaaataa ctgctaccaa ttttttgatg agagtaaaaa ctggtatgag      120

agccaggctt cttgtatgtc tcaaaatgcc agccttctga aagtatacag caaagaggac      180

caggatttac ttaaactggt gaagtcatat cattggatgg gactagtaca cattccaaca      240

aatggatctt ggcagtggga agatggctcc attctctcac ccaacctact aacaataatt      300

gaaatgcaga agggagactg tgcactctat gcctcgagct ttaaaggcta tatagaaaac      360

tgttcaactc caaatacgta catctgtatg caaaggactg tg                         402


<210>  6
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the NKG2D extra-cellular domain peptide

<400>  6

Phe Asn Gln Glu Val Gln Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro 
1               5                   10                  15      


Cys Pro Lys Asn Trp Ile Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe 
            20                  25                  30          


Asp Glu Ser Lys Asn Trp Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln 
        35                  40                  45              


Asn Ala Ser Leu Leu Lys Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu 
    50                  55                  60                  


Lys Leu Val Lys Ser Tyr His Trp Met Gly Leu Val His Ile Pro Thr 
65                  70                  75                  80  


Asn Gly Ser Trp Gln Trp Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu 
                85                  90                  95      


Leu Thr Ile Ile Glu Met Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser 
            100                 105                 110         


Ser Phe Lys Gly Tyr Ile Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile 
        115                 120                 125             


Cys Met Gln Arg Thr Val 
    130                 


<210>  7
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the IgG4 (UniProtKB - P01861) Hinge domain 
       (aa 99-110)

<400>  7
gaatctaaat atggcccccc ttgcccacca tgtccc                                 36


<210>  8
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the IgG4 (UniProtKB - P01861) Hinge domain
       (aa 99-110) peptide

<400>  8

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  9
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the CD28 (UniProtKB - P10747) transmembrane
       domain (aa 153-179)

<400>  9
ttctgggttt tggtagttgt cggcggggtg cttgcttgct attcattgtt ggttacggtg       60

gcttttataa ttttctgggt a                                                 81


<210>  10
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CD28 (UniProtKB - P10747) 
       transmembrane domain (aa 153-179) peptide

<400>  10

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  11
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the 4-1BB (UniProtKB - Q07011) 
       co-stimulatory signaling domain: 4-1BB  (aa 209-255)

<400>  11
agattctctg tggtgaagag gggtagaaag aagctcctct atatctttaa gcaacctttt       60

atgcggccag tacagactac tcaagaggag gacggatgtt catgccgatt ccccgaagag      120

gaagaagggg ggtgcgagtt g                                                141


<210>  12
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the 4-1BB (UniProtKB - Q07011) 
       co-stimulatory signaling domain: 4-1BB  (aa 209-255) peptide

<400>  12

Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
1               5                   10                  15      


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            20                  25                  30          


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40                  45          


<210>  13
<211>  156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the DAP12 (UniProtKB - O43914) 
       intracellular domain (aa 62-113)

<400>  13
tacttcctgg gacgacttgt tcctcggggg cggggtgctg cagaagctgc aacgcgaaaa       60

caaagaatta ccgagactga gagtccgtat caagaactgc agggccaaag aagcgacgtt      120

tactccgatc tcaataccca acggccttac tacaaa                                156


<210>  14
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the DAP12 (UniProtKB - O43914) 
       intracellular domain (aa 62-113) peptide

<400>  14

Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala 
1               5                   10                  15      


Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu 
            20                  25                  30          


Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg 
        35                  40                  45              


Pro Tyr Tyr Lys 
    50          


<210>  15
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM motif with ESP sequence

<400>  15

Glu Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser 
1               5                   10                  15      


Asp Leu 
        


<210>  16
<211>  343
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the NKG2Dbp CAR

<400>  16

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gly Ala His Ala Phe Asn Gln Glu Val Gln 
            20                  25                  30          


Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro Cys Pro Lys Asn Trp Ile 
        35                  40                  45              


Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe Asp Glu Ser Lys Asn Trp 
    50                  55                  60                  


Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln Asn Ala Ser Leu Leu Lys 
65                  70                  75                  80  


Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu Lys Leu Val Lys Ser Tyr 
                85                  90                  95      


His Trp Met Gly Leu Val His Ile Pro Thr Asn Gly Ser Trp Gln Trp 
            100                 105                 110         


Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu Leu Thr Ile Ile Glu Met 
        115                 120                 125             


Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser Ser Phe Lys Gly Tyr Ile 
    130                 135                 140                 


Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile Cys Met Gln Arg Thr Val 
145                 150                 155                 160 


Ala Ser Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro 
                165                 170                 175     


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
            180                 185                 190         


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
        195                 200                 205             


Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val Val 
    210                 215                 220                 


Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile 
225                 230                 235                 240 


Ile Phe Trp Val Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu 
                245                 250                 255     


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
            260                 265                 270         


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
        275                 280                 285             


Cys Glu Leu Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala 
    290                 295                 300                 


Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro 
305                 310                 315                 320 


Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn 
                325                 330                 335     


Thr Gln Arg Pro Tyr Tyr Lys 
            340             


<210>  17
<211>  4871
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of the plasmid for the construction of NKG2Dbz1 CAR

<400>  17
ggtctcctcc caataacgat aatagatacg gaacggacac gcagctagat taaccctaga       60

aagataatca tattgtgacg tacgttaaag ataatcatgt gtaaaattga cgcatgaaaa      120

cttttatgag ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg      180

ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc      240

ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc      300

ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaag      360

gcgggatcca ctgaggggta ccgctccggt gcccgtcagt gggcagagcg cacatcgccc      420

acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg      480

cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg      540

ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc      600

gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat      660

ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag      720

cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct      780

cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac      840

cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct      900

gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact      960

ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt     1020

tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct     1080

ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca     1140

aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct     1200

gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca     1260

caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg     1320

gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg     1380

ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg     1440

ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg     1500

ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga     1560

aagcttagct gaattctaat acgactcact atagggaaat aagagagaaa agaagagtaa     1620

gaagaaatat aagagccacc atgcttctcc tggtgacaag ccttctgctc tgtgagttac     1680

cacacccagc attcctcctg atcccaggcg cgcatgcctt caaccaagaa gttcaaattc     1740

ccttgaccga aagttactgt ggcccatgtc ctaaaaactg gatatgttac aaaaataact     1800

gctaccaatt ttttgatgag agtaaaaact ggtatgagag ccaggcttct tgtatgtctc     1860

aaaatgccag ccttctgaaa gtatacagca aagaggacca ggatttactt aaactggtga     1920

agtcatatca ttggatggga ctagtacaca ttccaacaaa tggatcttgg cagtgggaag     1980

atggctccat tctctcaccc aacctactaa caataattga aatgcagaag ggagactgtg     2040

cactctatgc ctcgagcttt aaaggctata tagaaaactg ttcaactcca aatacgtaca     2100

tctgtatgca aaggactgtg gctagcaact ggtcacaccc ccagtttgag aaaggtgggg     2160

gagggtcagg tggtgggggg tcaaattggt ctcatcccca gttcgagaag ggcggaggag     2220

gttcaggtgg gggtgggagc aactggagcc accctcaatt tgaaaaagga ggtggaggga     2280

gtggaggtgg tggatctgaa tctaaatatg gccccccttg cccaccatgt cccttctggg     2340

ttttggtagt tgtcggcggg gtgcttgctt gctattcatt gttggttacg gtggctttta     2400

taattttctg ggtaagattc tctgtggtga agaggggtag aaagaagctc ctctatatct     2460

ttaagcaacc ttttatgcgg ccagtacaga ctactcaaga ggaggacgga tgttcatgcc     2520

gattccccga agaggaagaa ggggggtgcg agttgagagt gaagttcagc aggagcgcag     2580

acgcccccgc gtaccagcag ggccagaacc agctctataa cgagctcaat ctaggacgaa     2640

gagaggagta cgatgttttg gacaagagac gtggccggga ccctgagatg gggggaaagc     2700

cgagaaggaa gaaccctcag gaaggcctgt gagtcgactg agtttggaca aaccacaact     2760

agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta     2820

accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag     2880

gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtact     2940

taagaatgca tgctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga     3000

gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac     3060

gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc     3120

tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg     3180

tccctgatat ctataacatg cgtcaatttt acgcagacta tctttctagg gttaatctag     3240

ctgcagtgta tgaaatgctt taaggaggag agggggagac caaagggcga gacgttaagg     3300

cctcacgtga catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt     3360

tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa     3420

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct     3480

ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc     3540

cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg     3600

tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct     3660

tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag     3720

cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga     3780

agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga     3840

agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accacgctgg     3900

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     3960

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     4020

gattttggtc atgccgtctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg     4080

cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag     4140

ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag     4200

ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca     4260

ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc     4320

gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag     4380

accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg     4440

gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt     4500

ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag     4560

ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt     4620

ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc     4680

ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga     4740

gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg     4800

agaacctgcg tgcaatccat cttgttcaat cataatatta ttgaagcatt tatcagggtt     4860

cgtctcgtcc c                                                          4871


<210>  18
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding the CD3 zeta ITAM-1 intracellular domain

<400>  18
gcccccgcgt accagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga       60

gaggagtacg atgttttgga caagaga                                           87


<210>  19
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CD3 zeta ITAM-1

<400>  19

Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
1               5                   10                  15      


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
            20                  25                  


<210>  20
<211>  363
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the NKG2Dbz1 CAR

<400>  20

Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      


Ala Phe Leu Leu Ile Pro Gly Ala His Ala Phe Asn Gln Glu Val Gln 
            20                  25                  30          


Ile Pro Leu Thr Glu Ser Tyr Cys Gly Pro Cys Pro Lys Asn Trp Ile 
        35                  40                  45              


Cys Tyr Lys Asn Asn Cys Tyr Gln Phe Phe Asp Glu Ser Lys Asn Trp 
    50                  55                  60                  


Tyr Glu Ser Gln Ala Ser Cys Met Ser Gln Asn Ala Ser Leu Leu Lys 
65                  70                  75                  80  


Val Tyr Ser Lys Glu Asp Gln Asp Leu Leu Lys Leu Val Lys Ser Tyr 
                85                  90                  95      


His Trp Met Gly Leu Val His Ile Pro Thr Asn Gly Ser Trp Gln Trp 
            100                 105                 110         


Glu Asp Gly Ser Ile Leu Ser Pro Asn Leu Leu Thr Ile Ile Glu Met 
        115                 120                 125             


Gln Lys Gly Asp Cys Ala Leu Tyr Ala Ser Ser Phe Lys Gly Tyr Ile 
    130                 135                 140                 


Glu Asn Cys Ser Thr Pro Asn Thr Tyr Ile Cys Met Gln Arg Thr Val 
145                 150                 155                 160 


Ala Ser Asn Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Gly Ser 
                165                 170                 175     


Gly Gly Gly Gly Ser Asn Trp Ser His Pro Gln Phe Glu Lys Gly Gly 
            180                 185                 190         


Gly Gly Ser Gly Gly Gly Gly Ser Asn Trp Ser His Pro Gln Phe Glu 
        195                 200                 205             


Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ser Lys Tyr Gly 
    210                 215                 220                 


Pro Pro Cys Pro Pro Cys Pro Phe Trp Val Leu Val Val Val Gly Gly 
225                 230                 235                 240 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                245                 250                 255     


Trp Val Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            260                 265                 270         


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        275                 280                 285             


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    290                 295                 300                 


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
305                 310                 315                 320 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
                325                 330                 335     


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
            340                 345                 350         


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
        355                 360             


<210>  21
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CD-8 alpha hinge

<400>  21

Phe Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro 
1               5                   10                  15      


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
            20                  25                  30          


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
        35                  40                  45              


Gly Leu Asp 
    50      


<210>  22
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleotide sequence of CD-8 alpha hinge

<400>  22
ttcgtgccgg tcttcctgcc agcgaagccc accacgacgc cagcgccgcg accaccaaca       60

ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc cagaggcgtg ccggccagcg      120

gcggggggcg cagtgcacac gagggggctg gac                                   153


<210>  23
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CD3 zeta ITAM2

<400>  23

Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
1               5                   10                  15      


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
            20                  25                  


<210>  24
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleotide sequence of CD3 zeta ITAM2

<400>  24
ccgcaaagga ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa agataagatg       60

gcggaggcct acagtgagat tgggatg                                           87


<210>  25
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of CD3 zeta ITAM3

<400>  25

Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
1               5                   10                  15      


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            20                  25                  


<210>  26
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Exemplary nucleotide sequence of CD3 zeta ITAM3

<400>  26
gagcgccgga ggggcaaggg gcacgatggc ctttaccagg gtctcagtac agccaccaag       60

gacacctacg acgcccttca catgcaggc                                         89


<210>  27
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the CD3 zeta ITAM-1 intracellular 
       signaling  domain

<400>  27

Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
1               5                   10                  15      


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
            20                  25                  30          


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
        35                  40                  45              


Leu 
    


