                         SEQUENCE LISTING

<110>  Aeglea BioTherapeutics, Inc.
 
<120>  METHODS FOR PRODUCTION OF HUMAN RECOMBINANT ARGINASE 1 AND USES 
       THEREOF

<130>  A0016

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  321
<212>  PRT
<213>  Homo sapiens

<400>  1

Ser Ala Lys Ser Arg Thr Ile Gly Ile Ile Gly Ala Pro Phe Ser Lys 
1               5                   10                  15      


Gly Gln Pro Arg Gly Gly Val Glu Glu Gly Pro Thr Val Leu Arg Lys 
            20                  25                  30          


Ala Gly Leu Leu Glu Lys Leu Lys Glu Gln Glu Cys Asp Val Lys Asp 
        35                  40                  45              


Tyr Gly Asp Leu Pro Phe Ala Asp Ile Pro Asn Asp Ser Pro Phe Gln 
    50                  55                  60                  


Ile Val Lys Asn Pro Arg Ser Val Gly Lys Ala Ser Glu Gln Leu Ala 
65                  70                  75                  80  


Gly Lys Val Ala Glu Val Lys Lys Asn Gly Arg Ile Ser Leu Val Leu 
                85                  90                  95      


Gly Gly Asp His Ser Leu Ala Ile Gly Ser Ile Ser Gly His Ala Arg 
            100                 105                 110         


Val His Pro Asp Leu Gly Val Ile Trp Val Asp Ala His Thr Asp Ile 
        115                 120                 125             


Asn Thr Pro Leu Thr Thr Thr Ser Gly Asn Leu His Gly Gln Pro Val 
    130                 135                 140                 


Ser Phe Leu Leu Lys Glu Leu Lys Gly Lys Ile Pro Asp Val Pro Gly 
145                 150                 155                 160 


Phe Ser Trp Val Thr Pro Cys Ile Ser Ala Lys Asp Ile Val Tyr Ile 
                165                 170                 175     


Gly Leu Arg Asp Val Asp Pro Gly Glu His Tyr Ile Leu Lys Thr Leu 
            180                 185                 190         


Gly Ile Lys Tyr Phe Ser Met Thr Glu Val Asp Arg Leu Gly Ile Gly 
        195                 200                 205             


Lys Val Met Glu Glu Thr Leu Ser Tyr Leu Leu Gly Arg Lys Lys Arg 
    210                 215                 220                 


Pro Ile His Leu Ser Phe Asp Val Asp Gly Leu Asp Pro Ser Phe Thr 
225                 230                 235                 240 


Pro Ala Thr Gly Thr Pro Val Val Gly Gly Leu Thr Tyr Arg Glu Gly 
                245                 250                 255     


Leu Tyr Ile Thr Glu Glu Ile Tyr Lys Thr Gly Leu Leu Ser Gly Leu 
            260                 265                 270         


Asp Ile Met Glu Val Asn Pro Ser Leu Gly Lys Thr Pro Glu Glu Val 
        275                 280                 285             


Thr Arg Thr Val Asn Thr Ala Val Ala Ile Thr Leu Ala Cys Phe Gly 
    290                 295                 300                 


Leu Ala Arg Glu Gly Asn His Lys Pro Ile Asp Tyr Leu Asn Pro Pro 
305                 310                 315                 320 


Lys 
    


<210>  2
<211>  969
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon Optimized DNA Sequence

<400>  2
atgtctgcga agagccgtac gatcggcatt attggtgcgc cgttctctaa aggtcagcca       60

cgcggtggtg tggaagaggg tccgacggtt ctgcgtaagg ccggtttatt agaaaagctg      120

aaagagcagg agtgcgacgt taaggactac ggtgacttac cattcgcgga catcccgaat      180

gatagcccgt tccaaatcgt taagaatccg cgctctgtgg gtaaagcaag cgagcagtta      240

gcaggtaagg tggccgaggt caagaaaaac ggtcgtatta gcctggtttt aggcggtgat      300

catagcttag caattggctc tatctctggt catgcccgtg tgcacccaga tttaggtgtc      360

atttgggttg acgcccatac ggatatcaat acgccattaa cgaccaccag cggcaatctg      420

catggccagc cggttagctt cttactgaag gagctgaagg gtaaaattcc agatgttccg      480

ggctttagct gggtcacgcc atgtatttct gccaaggata tcgtgtacat tggcttacgt      540

gacgtcgacc caggtgagca ctacatctta aagaccctgg gtatcaagta tttcagcatg      600

acggaagtgg accgcttagg catcggcaag gtgatggagg agacgctgag ctatctgctg      660

ggccgtaaga aacgtccaat ccatctgagc ttcgatgttg acggcttaga cccgagcttt      720

acgccagcca ccggcacgcc ggtcgttggt ggtttaacgt atcgcgaagg cctgtatatc      780

acggaggaaa tctataagac gggtttactg agcggtctgg acattatgga ggttaatcca      840

agcttaggta agacgccgga agaagttacc cgtaccgtta acacggcggt cgcgatcacg      900

ttagcatgtt tcggtttagc ccgcgagggc aaccataaac caattgatta tctgaatcca      960

ccgaagtga                                                              969


<210>  3
<211>  353
<212>  PRT
<213>  Homo sapiens

<400>  3

Ser Leu Arg Gly Ser Leu Ser Arg Leu Leu Gln Thr Arg Val His Ser 
1               5                   10                  15      


Ile Leu Lys Lys Ser Val His Ser Val Ala Val Ile Gly Ala Pro Phe 
            20                  25                  30          


Ser Gln Gly Gln Lys Arg Lys Gly Val Glu His Gly Pro Ala Ala Ile 
        35                  40                  45              


Arg Glu Ala Gly Leu Met Lys Arg Leu Ser Ser Leu Gly Cys His Leu 
    50                  55                  60                  


Lys Asp Phe Gly Asp Leu Ser Phe Thr Pro Val Pro Lys Asp Asp Leu 
65                  70                  75                  80  


Tyr Asn Asn Leu Ile Val Asn Pro Arg Ser Val Gly Leu Ala Asn Gln 
                85                  90                  95      


Glu Leu Ala Glu Val Val Ser Arg Ala Val Ser Asp Gly Tyr Ser Cys 
            100                 105                 110         


Val Thr Leu Gly Gly Asp His Ser Leu Ala Ile Gly Thr Ile Ser Gly 
        115                 120                 125             


His Ala Arg His Cys Pro Asp Leu Cys Val Val Trp Val Asp Ala His 
    130                 135                 140                 


Ala Asp Ile Asn Thr Pro Leu Thr Thr Ser Ser Gly Asn Leu His Gly 
145                 150                 155                 160 


Gln Pro Val Ser Phe Leu Leu Arg Glu Leu Gln Asp Lys Val Pro Gln 
                165                 170                 175     


Leu Pro Gly Phe Ser Trp Ile Lys Pro Cys Ile Ser Ser Ala Ser Ile 
            180                 185                 190         


Val Tyr Ile Gly Leu Arg Asp Val Asp Pro Pro Glu His Phe Ile Leu 
        195                 200                 205             


Lys Asn Tyr Asp Ile Gln Tyr Phe Ser Met Arg Asp Ile Asp Arg Leu 
    210                 215                 220                 


Gly Ile Gln Lys Val Met Glu Arg Thr Phe Asp Leu Leu Ile Gly Lys 
225                 230                 235                 240 


Arg Gln Arg Pro Ile His Leu Ser Phe Asp Ile Asp Ala Phe Asp Pro 
                245                 250                 255     


Thr Leu Ala Pro Ala Thr Gly Thr Pro Val Val Gly Gly Leu Thr Tyr 
            260                 265                 270         


Arg Glu Gly Met Tyr Ile Ala Glu Glu Ile His Asn Thr Gly Leu Leu 
        275                 280                 285             


Ser Ala Leu Asp Leu Val Glu Val Asn Pro Gln Leu Ala Thr Ser Glu 
    290                 295                 300                 


Glu Glu Ala Lys Thr Thr Ala Asn Leu Ala Val Asp Val Ile Ala Ser 
305                 310                 315                 320 


Ser Phe Gly Gln Thr Arg Glu Gly Gly His Ile Val Tyr Asp Gln Leu 
                325                 330                 335     


Pro Thr Pro Ser Ser Pro Asp Glu Ser Glu Asn Gln Ala Arg Val Arg 
            340                 345                 350         


Ile 
    


