			SEQUENCE LISTING

<110> CODEXIS, INC.
      SOCIETE DES PRODUITS NESTLE S.A.
 
<120> ENGINEERED LIPASE VARIANTS
 
<130> CX7-187WO2
 
<140> TO BE ASSIGNED
<141> 2020-08-28
 
<150> US 62/894,019
<151> 2019-08-30
 
<160> 1796
 
<210> 1
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Wild type triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaata a                                           1161

 
<210> 2
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Wild type triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 2
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 3
<211> 1353
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Pancreatic Triacylglycerol Lipase from Homo sapiens
 
<400> 3
atgaaggaag tttgctacga gcgtctgggt tgtttcagcg atgacagccc gtggagcggt 60
atcaccgaac gtccgctgca cattttgccg tggagcccga aagacgttaa cacccgtttc 120
ctgttgtata ccaacgaaaa tccgaacaat tttcaagagg ttgctgcgga tagcagcagc 180
atcagcggta gcaacttcaa gaccaatcgt aaaacccgtt tcattatcca tggttttatt 240
gacaagggtg aagagaactg gctggctaac gtttgcaaga acttgtttaa agttgaaagc 300
gttaactgca tctgtgttga ttggaaaggt ggtagccgta ccggttacac ccaagctagc 360
cagaacattc gtatcgttgg tgctgaagtt gcgtacttcg ttgagtttct gcaaagcgcg 420
tttggttata gcccgagcaa tgttcacgtt attggtcatt ctctgggtgc tcacgctgcg 480
ggtgaggcgg gtcgtcgtac caacggtacc attggtcgta tcaccggtct ggacccggcg 540
gaaccgtgtt tccagggtac cccggagctg gttcgtttgg acccgagcga tgctaagttt 600
gttgacgtta tccacaccga tggtgcgccg attgttccga acctgggttt cggtatgagc 660
caagttgttg gtcatttgga cttctttccg aatggtggtg ttgaaatgcc gggttgcaag 720
aaaaacatcc tgagccagat tgttgatatc gacggtattt gggagggtac ccgtgatttt 780
gctgcgtgta atcacttgcg tagctacaag tactataccg acagcattgt taacccggat 840
ggtttcgctg gttttccgtg cgcgagctat aatgttttca ccgctaacaa atgctttccg 900
tgtccgagcg gtggttgtcc gcaaatgggt cattacgcgg accgttatcc gggtaaaacc 960
aatgatgttg gtcagaaatt ctacctggac accggtgacg ctagcaactt tgcgcgttgg 1020
cgttataagg ttagcgttac cttgagcggt aaaaaggtta ccggtcacat cctggttagc 1080
ttgttcggta acaagggtaa tagcaagcaa tacgaaattt ttaagggtac cctgaaaccg 1140
gacagcaccc atagcaatga gttcgatagc gacgttgatg ttggtgacct gcaaatggtt 1200
aagttcattt ggtacaacaa cgttattaac ccgaccttgc cgcgtgttgg tgctagcaag 1260
attatcgttg aaaccaatgt tggtaaacag ttcaactttt gcagcccgga gaccgttcgt 1320
gaagaggttc tgttgacctt gaccccgtgt taa                              1353

 
<210> 4
<211> 450
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Pancreatic Triacylglycerol Lipase from Homo sapiens
 
<400> 4
Met Lys Glu Val Cys Tyr Glu Arg Leu Gly Cys Phe Ser Asp Asp Ser 
1               5                   10                  15      
Pro Trp Ser Gly Ile Thr Glu Arg Pro Leu His Ile Leu Pro Trp Ser 
            20                  25                  30          
Pro Lys Asp Val Asn Thr Arg Phe Leu Leu Tyr Thr Asn Glu Asn Pro 
        35                  40                  45              
Asn Asn Phe Gln Glu Val Ala Ala Asp Ser Ser Ser Ile Ser Gly Ser 
    50                  55                  60                  
Asn Phe Lys Thr Asn Arg Lys Thr Arg Phe Ile Ile His Gly Phe Ile 
65                  70                  75                  80  
Asp Lys Gly Glu Glu Asn Trp Leu Ala Asn Val Cys Lys Asn Leu Phe 
                85                  90                  95      
Lys Val Glu Ser Val Asn Cys Ile Cys Val Asp Trp Lys Gly Gly Ser 
            100                 105                 110         
Arg Thr Gly Tyr Thr Gln Ala Ser Gln Asn Ile Arg Ile Val Gly Ala 
        115                 120                 125             
Glu Val Ala Tyr Phe Val Glu Phe Leu Gln Ser Ala Phe Gly Tyr Ser 
    130                 135                 140                 
Pro Ser Asn Val His Val Ile Gly His Ser Leu Gly Ala His Ala Ala 
145                 150                 155                 160 
Gly Glu Ala Gly Arg Arg Thr Asn Gly Thr Ile Gly Arg Ile Thr Gly 
                165                 170                 175     
Leu Asp Pro Ala Glu Pro Cys Phe Gln Gly Thr Pro Glu Leu Val Arg 
            180                 185                 190         
Leu Asp Pro Ser Asp Ala Lys Phe Val Asp Val Ile His Thr Asp Gly 
        195                 200                 205             
Ala Pro Ile Val Pro Asn Leu Gly Phe Gly Met Ser Gln Val Val Gly 
    210                 215                 220                 
His Leu Asp Phe Phe Pro Asn Gly Gly Val Glu Met Pro Gly Cys Lys 
225                 230                 235                 240 
Lys Asn Ile Leu Ser Gln Ile Val Asp Ile Asp Gly Ile Trp Glu Gly 
                245                 250                 255     
Thr Arg Asp Phe Ala Ala Cys Asn His Leu Arg Ser Tyr Lys Tyr Tyr 
            260                 265                 270         
Thr Asp Ser Ile Val Asn Pro Asp Gly Phe Ala Gly Phe Pro Cys Ala 
        275                 280                 285             
Ser Tyr Asn Val Phe Thr Ala Asn Lys Cys Phe Pro Cys Pro Ser Gly 
    290                 295                 300                 
Gly Cys Pro Gln Met Gly His Tyr Ala Asp Arg Tyr Pro Gly Lys Thr 
305                 310                 315                 320 
Asn Asp Val Gly Gln Lys Phe Tyr Leu Asp Thr Gly Asp Ala Ser Asn 
                325                 330                 335     
Phe Ala Arg Trp Arg Tyr Lys Val Ser Val Thr Leu Ser Gly Lys Lys 
            340                 345                 350         
Val Thr Gly His Ile Leu Val Ser Leu Phe Gly Asn Lys Gly Asn Ser 
        355                 360                 365             
Lys Gln Tyr Glu Ile Phe Lys Gly Thr Leu Lys Pro Asp Ser Thr His 
    370                 375                 380                 
Ser Asn Glu Phe Asp Ser Asp Val Asp Val Gly Asp Leu Gln Met Val 
385                 390                 395                 400 
Lys Phe Ile Trp Tyr Asn Asn Val Ile Asn Pro Thr Leu Pro Arg Val 
                405                 410                 415     
Gly Ala Ser Lys Ile Ile Val Glu Thr Asn Val Gly Lys Gln Phe Asn 
            420                 425                 430         
Phe Cys Ser Pro Glu Thr Val Arg Glu Glu Val Leu Leu Thr Leu Thr 
        435                 440                 445             
Pro Cys                                                         
    450                                                         

 
<210> 5
<211> 1353
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Pancreatic Triacylglycerol Lipase from Panthera tigris altaica
 
<400> 5
atgaaggaag tttgcttccc gcgtctgggt tgttttagcg atgacgctcc gtgggcgggt 60
atcgttcaac gtccgctgaa gattttgccg tgggctccga aagacgttaa cacccgtttc 120
ctgttgtaca ccaacgagaa tccggataat tttcaggaat tgaccgctga cgcgagcatt 180
atcaccgata gcaacttcaa gaccaatcgt aaaacccgtt tcattatcca cggttttatc 240
gacaagggtg aagagagctg gctggcgaac atttgcaaaa atttgtttac cgttgagagc 300
gttaactgca tctgtgttga ttggaagagc ggtagccgta ccggttatac ccaagctagc 360
cagaatattc gtatcgttgg tgctgaggtt gcgtacttcg ttgaagttct gaaaagcgcg 420
ttgggttata gcccgagcaa cgttcacatt atcggtcata gcctgggtag ccatgctgcg 480
ggtgaagctg gtcgtcgtac caatggtacc gttggtcgta tcaccggttt ggacccggcg 540
gaaccgtgtt tcgagggtac cccggaactg gttcgtttgg acccgagcga tgctcaattt 600
gttgacgtta ttcacaccga tgctgcgccg attatcccga acatgggttt cggtatgagc 660
cagaatgttg gtcatctgga tttctttccg aacggtggta aagagatgcc gggttgccaa 720
aagaacatct tgagccagat tgttgatatc gacggtattt gggaaggtac ccgtgacttt 780
gttgcgtgta accacctgcg tagctacaag tactatagcg atagcatttt gaatccgacc 840
ggtttcgctg gttttccgtg cgcgagctat agcgttttca ccgctaacaa gtgctttccg 900
tgtccgagcg agggttgtcc gcaaatgggt cattacgcgg accgttttag cggtaaaacc 960
aacggtgtta atcaggtttt ctacctgaac accggtgacg ctagcgattt tgcgcgttgg 1020
cgttataagg ttgctgttac cttgagcggt aaaaaggtta ccggtcacgt tctggttagc 1080
ttgttcggta acaagggtaa tagcaaacaa tacgagattt tcaagggtac cctgcaggcg 1140
gacaacaccc atagcaatga atttgatagc gacgttgatg ttggtgacct gcaaaaggtt 1200
aagttcattt ggtacaacaa cgttattaac ccgaccttgc cgcgtgttgg tgctagcaaa 1260
atcgttgttg aacgtaacga cggtattagc ttcaattttt gcagccagga gaccgttcgt 1320
gaagatgttc tgttgaccct gaccccgtgt taa                              1353

 
<210> 6
<211> 450
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Pancreatic Triacylglycerol Lipase from Panthera tigris altaica
 
<400> 6
Met Lys Glu Val Cys Phe Pro Arg Leu Gly Cys Phe Ser Asp Asp Ala 
1               5                   10                  15      
Pro Trp Ala Gly Ile Val Gln Arg Pro Leu Lys Ile Leu Pro Trp Ala 
            20                  25                  30          
Pro Lys Asp Val Asn Thr Arg Phe Leu Leu Tyr Thr Asn Glu Asn Pro 
        35                  40                  45              
Asp Asn Phe Gln Glu Leu Thr Ala Asp Ala Ser Ile Ile Thr Asp Ser 
    50                  55                  60                  
Asn Phe Lys Thr Asn Arg Lys Thr Arg Phe Ile Ile His Gly Phe Ile 
65                  70                  75                  80  
Asp Lys Gly Glu Glu Ser Trp Leu Ala Asn Ile Cys Lys Asn Leu Phe 
                85                  90                  95      
Thr Val Glu Ser Val Asn Cys Ile Cys Val Asp Trp Lys Ser Gly Ser 
            100                 105                 110         
Arg Thr Gly Tyr Thr Gln Ala Ser Gln Asn Ile Arg Ile Val Gly Ala 
        115                 120                 125             
Glu Val Ala Tyr Phe Val Glu Val Leu Lys Ser Ala Leu Gly Tyr Ser 
    130                 135                 140                 
Pro Ser Asn Val His Ile Ile Gly His Ser Leu Gly Ser His Ala Ala 
145                 150                 155                 160 
Gly Glu Ala Gly Arg Arg Thr Asn Gly Thr Val Gly Arg Ile Thr Gly 
                165                 170                 175     
Leu Asp Pro Ala Glu Pro Cys Phe Glu Gly Thr Pro Glu Leu Val Arg 
            180                 185                 190         
Leu Asp Pro Ser Asp Ala Gln Phe Val Asp Val Ile His Thr Asp Ala 
        195                 200                 205             
Ala Pro Ile Ile Pro Asn Met Gly Phe Gly Met Ser Gln Asn Val Gly 
    210                 215                 220                 
His Leu Asp Phe Phe Pro Asn Gly Gly Lys Glu Met Pro Gly Cys Gln 
225                 230                 235                 240 
Lys Asn Ile Leu Ser Gln Ile Val Asp Ile Asp Gly Ile Trp Glu Gly 
                245                 250                 255     
Thr Arg Asp Phe Val Ala Cys Asn His Leu Arg Ser Tyr Lys Tyr Tyr 
            260                 265                 270         
Ser Asp Ser Ile Leu Asn Pro Thr Gly Phe Ala Gly Phe Pro Cys Ala 
        275                 280                 285             
Ser Tyr Ser Val Phe Thr Ala Asn Lys Cys Phe Pro Cys Pro Ser Glu 
    290                 295                 300                 
Gly Cys Pro Gln Met Gly His Tyr Ala Asp Arg Phe Ser Gly Lys Thr 
305                 310                 315                 320 
Asn Gly Val Asn Gln Val Phe Tyr Leu Asn Thr Gly Asp Ala Ser Asp 
                325                 330                 335     
Phe Ala Arg Trp Arg Tyr Lys Val Ala Val Thr Leu Ser Gly Lys Lys 
            340                 345                 350         
Val Thr Gly His Val Leu Val Ser Leu Phe Gly Asn Lys Gly Asn Ser 
        355                 360                 365             
Lys Gln Tyr Glu Ile Phe Lys Gly Thr Leu Gln Ala Asp Asn Thr His 
    370                 375                 380                 
Ser Asn Glu Phe Asp Ser Asp Val Asp Val Gly Asp Leu Gln Lys Val 
385                 390                 395                 400 
Lys Phe Ile Trp Tyr Asn Asn Val Ile Asn Pro Thr Leu Pro Arg Val 
                405                 410                 415     
Gly Ala Ser Lys Ile Val Val Glu Arg Asn Asp Gly Ile Ser Phe Asn 
            420                 425                 430         
Phe Cys Ser Gln Glu Thr Val Arg Glu Asp Val Leu Leu Thr Leu Thr 
        435                 440                 445             
Pro Cys                                                         
    450                                                         

 
<210> 7
<211> 1362
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Pancreatic Lipase-Related Protein 2 from Homo sapiens
 
<400> 7
atgaaagaag tttgctatgg tcaactgggt tgtttcagcg atgagaagcc gtgggctggt 60
accttgcagc gtccggttaa actgttgccg tggagcccgg aagatatcga cacccgtttc 120
ctgttgtaca ccaacgagaa tccgaacaat tttcaactga tcaccggtac cgaaccggat 180
accattgagg ctagcaactt ccagctggac cgtaagaccc gtttcattat ccacggtttc 240
ctggataaag cggaagacag ctggccgagc gatatgtgca agaaaatgtt tgaagttgag 300
aaggttaact gcatttgtgt tgactggcgt catggtagcc gtgctatgta cacccaagcg 360
gttcagaata tccgtgttgt tggtgctgag accgcgtttc tgattcaagc tctgagcacc 420
cagctgggtt atagcttgga agatgttcac gttatcggtc attctttggg tgctcacacc 480
gctgcggagg cgggtcgtcg tctgggtggt cgtgttggtc gtattaccgg tctggacccg 540
gctggtccgt gtttccaaga cgaaccggaa gaagttcgtc tggacccgag cgacgcggtt 600
tttgttgatg ttatccacac cgacagcagc ccgattgttc cgagcctggg tttcggtatg 660
agccagaagg ttggtcattt ggacttcttt ccgaacggtg gtaaagagat gccgggttgc 720
aagaaaaatg ttctgagcac cattaccgat atcgacggta tttgggaagg tatcggtggt 780
ttcgttagct gtaaccactt gcgtagcttt gagtactaca gcagcagcgt tctgaatccg 840
gatggtttcc tgggttaccc gtgcgctagc tatgacgaat ttcaagagag caagtgcttt 900
ccgtgtccgg cggaaggttg tccgaaaatg ggtcattacg ctgatcaatt caagggtaaa 960
accagcgcgg ttgaacagac cttctttctg aacaccggcg agagcggtaa ttttaccagc 1020
tggcgttata aggttagcgt taccctgagc ggtaaagaga aagttaacgg ttacattcgt 1080
atcgctttgt atggtagcaa cgaaaatagc aaacaatacg agattttcaa gggtagcctg 1140
aaaccggacg ctagccatac ctgcgcgatc gatgttgact tcaacgttgg taaaatccag 1200
aaggttaagt tcttgtggaa caagcgtggt atcaatctga gcgaaccgaa attgggtgcg 1260
agccaaatta ccgttcagag cggtgaagat ggtaccgagt acaacttttg cagcagcgac 1320
accgttgaag agaatgttct gcagagcttg tatccgtgtt aa                    1362

 
<210> 8
<211> 453
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Pancreatic Lipase-Related Protein 2 from Homo sapiens
 
<400> 8
Met Lys Glu Val Cys Tyr Gly Gln Leu Gly Cys Phe Ser Asp Glu Lys 
1               5                   10                  15      
Pro Trp Ala Gly Thr Leu Gln Arg Pro Val Lys Leu Leu Pro Trp Ser 
            20                  25                  30          
Pro Glu Asp Ile Asp Thr Arg Phe Leu Leu Tyr Thr Asn Glu Asn Pro 
        35                  40                  45              
Asn Asn Phe Gln Leu Ile Thr Gly Thr Glu Pro Asp Thr Ile Glu Ala 
    50                  55                  60                  
Ser Asn Phe Gln Leu Asp Arg Lys Thr Arg Phe Ile Ile His Gly Phe 
65                  70                  75                  80  
Leu Asp Lys Ala Glu Asp Ser Trp Pro Ser Asp Met Cys Lys Lys Met 
                85                  90                  95      
Phe Glu Val Glu Lys Val Asn Cys Ile Cys Val Asp Trp Arg His Gly 
            100                 105                 110         
Ser Arg Ala Met Tyr Thr Gln Ala Val Gln Asn Ile Arg Val Val Gly 
        115                 120                 125             
Ala Glu Thr Ala Phe Leu Ile Gln Ala Leu Ser Thr Gln Leu Gly Tyr 
    130                 135                 140                 
Ser Leu Glu Asp Val His Val Ile Gly His Ser Leu Gly Ala His Thr 
145                 150                 155                 160 
Ala Ala Glu Ala Gly Arg Arg Leu Gly Gly Arg Val Gly Arg Ile Thr 
                165                 170                 175     
Gly Leu Asp Pro Ala Gly Pro Cys Phe Gln Asp Glu Pro Glu Glu Val 
            180                 185                 190         
Arg Leu Asp Pro Ser Asp Ala Val Phe Val Asp Val Ile His Thr Asp 
        195                 200                 205             
Ser Ser Pro Ile Val Pro Ser Leu Gly Phe Gly Met Ser Gln Lys Val 
    210                 215                 220                 
Gly His Leu Asp Phe Phe Pro Asn Gly Gly Lys Glu Met Pro Gly Cys 
225                 230                 235                 240 
Lys Lys Asn Val Leu Ser Thr Ile Thr Asp Ile Asp Gly Ile Trp Glu 
                245                 250                 255     
Gly Ile Gly Gly Phe Val Ser Cys Asn His Leu Arg Ser Phe Glu Tyr 
            260                 265                 270         
Tyr Ser Ser Ser Val Leu Asn Pro Asp Gly Phe Leu Gly Tyr Pro Cys 
        275                 280                 285             
Ala Ser Tyr Asp Glu Phe Gln Glu Ser Lys Cys Phe Pro Cys Pro Ala 
    290                 295                 300                 
Glu Gly Cys Pro Lys Met Gly His Tyr Ala Asp Gln Phe Lys Gly Lys 
305                 310                 315                 320 
Thr Ser Ala Val Glu Gln Thr Phe Phe Leu Asn Thr Gly Glu Ser Gly 
                325                 330                 335     
Asn Phe Thr Ser Trp Arg Tyr Lys Val Ser Val Thr Leu Ser Gly Lys 
            340                 345                 350         
Glu Lys Val Asn Gly Tyr Ile Arg Ile Ala Leu Tyr Gly Ser Asn Glu 
        355                 360                 365             
Asn Ser Lys Gln Tyr Glu Ile Phe Lys Gly Ser Leu Lys Pro Asp Ala 
    370                 375                 380                 
Ser His Thr Cys Ala Ile Asp Val Asp Phe Asn Val Gly Lys Ile Gln 
385                 390                 395                 400 
Lys Val Lys Phe Leu Trp Asn Lys Arg Gly Ile Asn Leu Ser Glu Pro 
                405                 410                 415     
Lys Leu Gly Ala Ser Gln Ile Thr Val Gln Ser Gly Glu Asp Gly Thr 
            420                 425                 430         
Glu Tyr Asn Phe Cys Ser Ser Asp Thr Val Glu Glu Asn Val Leu Gln 
        435                 440                 445             
Ser Leu Tyr Pro Cys                                             
    450                                                         

 
<210> 9
<211> 1143
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Gastric lipase from Canis lupus familiaris
 
<400> 9
atgttgttcg gtaaactgca cccgaccaac ccggaagtta ccatgaatat cagccaaatg 60
attacctact ggggttatcc ggctgaagag tacgaagttg ttaccgagga cggttatatt 120
ctgggtatcg atcgtattcc gtacggtcgt aagaacagcg agaatatcgg tcgtcgtccg 180
gttgcgttcc tgcagcatgg tctgttggct agcgcgacca actggatcag caatttgccg 240
aacaatagct tggcttttat tctggctgat gcgggttatg acgtttggct gggtaacagc 300
cgtggtaata cctgggcgcg tcgtaacttg tactatagcc cggacagcgt tgaattttgg 360
gctttcagct ttgatgagat ggcgaaatac gacttgccgg ctaccatcga ttttattctg 420
aagaaaaccg gtcaggataa gttgcactat gttggtcata gccagggtac caccatcggt 480
ttcattgcgt ttagcaccaa cccgaagctg gctaaacgta ttaagacctt ctacgctttg 540
gcgccggttg cgaccgttaa atataccgaa accctgttga acaagttgat gctggttccg 600
agcttcttgt ttaaactgat cttcggtaat aagattttct acccgcacca tttctttgac 660
caatttctgg cgaccgaagt ttgcagccgt gagaccgttg atctgctgtg cagcaacgct 720
ctgttcatta tctgtggttt tgacaccatg aacttgaata tgagccgttt ggatgtttac 780
ctgagccaca acccggctgg taccagcgtt caaaatgttc tgcattggag ccaggcggtt 840
aaaagcggta aattccaggc ttttgactgg ggtagcccgg ttcaaaacat gatgcactat 900
catcagagca tgccgccata ctataatctg accgatatgc acgttccgat cgcggtttgg 960
aacggtggta atgatctgct ggctgacccg catgatgttg acctgttgct gagcaaattg 1020
ccgaacctga tctaccaccg taagattccg ccgtataatc atctggactt tatctgggct 1080
atggatgcgc cgcaagctgt ttacaacgag attgttagca tgatgggtac cgacaacaag 1140
taa                                                               1143

 
<210> 10
<211> 380
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Gastric lipase from Canis lupus familiaris
 
<400> 10
Met Leu Phe Gly Lys Leu His Pro Thr Asn Pro Glu Val Thr Met Asn 
1               5                   10                  15      
Ile Ser Gln Met Ile Thr Tyr Trp Gly Tyr Pro Ala Glu Glu Tyr Glu 
            20                  25                  30          
Val Val Thr Glu Asp Gly Tyr Ile Leu Gly Ile Asp Arg Ile Pro Tyr 
        35                  40                  45              
Gly Arg Lys Asn Ser Glu Asn Ile Gly Arg Arg Pro Val Ala Phe Leu 
    50                  55                  60                  
Gln His Gly Leu Leu Ala Ser Ala Thr Asn Trp Ile Ser Asn Leu Pro 
65                  70                  75                  80  
Asn Asn Ser Leu Ala Phe Ile Leu Ala Asp Ala Gly Tyr Asp Val Trp 
                85                  90                  95      
Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala Arg Arg Asn Leu Tyr Tyr 
            100                 105                 110         
Ser Pro Asp Ser Val Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala 
        115                 120                 125             
Lys Tyr Asp Leu Pro Ala Thr Ile Asp Phe Ile Leu Lys Lys Thr Gly 
    130                 135                 140                 
Gln Asp Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly 
145                 150                 155                 160 
Phe Ile Ala Phe Ser Thr Asn Pro Lys Leu Ala Lys Arg Ile Lys Thr 
                165                 170                 175     
Phe Tyr Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Glu Thr Leu 
            180                 185                 190         
Leu Asn Lys Leu Met Leu Val Pro Ser Phe Leu Phe Lys Leu Ile Phe 
        195                 200                 205             
Gly Asn Lys Ile Phe Tyr Pro His His Phe Phe Asp Gln Phe Leu Ala 
    210                 215                 220                 
Thr Glu Val Cys Ser Arg Glu Thr Val Asp Leu Leu Cys Ser Asn Ala 
225                 230                 235                 240 
Leu Phe Ile Ile Cys Gly Phe Asp Thr Met Asn Leu Asn Met Ser Arg 
                245                 250                 255     
Leu Asp Val Tyr Leu Ser His Asn Pro Ala Gly Thr Ser Val Gln Asn 
            260                 265                 270         
Val Leu His Trp Ser Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Phe 
        275                 280                 285             
Asp Trp Gly Ser Pro Val Gln Asn Met Met His Tyr His Gln Ser Met 
    290                 295                 300                 
Pro Pro Tyr Tyr Asn Leu Thr Asp Met His Val Pro Ile Ala Val Trp 
305                 310                 315                 320 
Asn Gly Gly Asn Asp Leu Leu Ala Asp Pro His Asp Val Asp Leu Leu 
                325                 330                 335     
Leu Ser Lys Leu Pro Asn Leu Ile Tyr His Arg Lys Ile Pro Pro Tyr 
            340                 345                 350         
Asn His Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Ala Val Tyr 
        355                 360                 365             
Asn Glu Ile Val Ser Met Met Gly Thr Asp Asn Lys                 
    370                 375                 380                 

 
<210> 11
<211> 1143
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Gastric lipase from Leptonychotes weddellii
 
<400> 11
atgtacttcg gtaaactgca cccgatcagc ccggaagtta ccatgaacat cagccaaatg 60
attacctact ggggttatcc gagcgaagag tacgatgttg ttaccgaaga cggttatatt 120
ctgggtatca atcgtattcc gtacggtaaa aagaacagcg agaatattgg tcgtcgtccg 180
gttgctttcc tgcagcatgg tctgttggct agcgcgacca actggatcgc taatttgccg 240
aacaatagct tggcgtttat tctggctgat gcgggttacg acgtttggct gggtaacagc 300
cgtggtaata cctgggctcg tcgtaacttg tatttcagcc cggacagcgt tgaattttgg 360
gcgttcagct ttgatgagat ggctaaatac gacttgccgg cgaccatcga tttcattctg 420
aagaaaaccg gtcaggataa gttgcactat gttggtcata gccagggtac caccatcggt 480
ttcattgctt ttagcaccaa cccgaagctg gcgaaacgta tcaagacctt ttacgctttg 540
gcgccggttg ctaccgttaa atataccaag agcctgttga acaagctggc gctggttccg 600
agcttcttgt ttaaactgat cttcggtgac aagattttct ttccgcacca attctttgaa 660
cagtttctgg ctaccgaagt ttgcagccgt gagaccgttg ctctgttgtg cagcaacgcg 720
ctgttcatta tctgtggttt tgacaccaag aacttgaatg ttagccgttt ggatgtttac 780
ctgagccaca atccggctgg taccagcgtt caagacgttc tgcattggag ccaggcggtt 840
aaaagcggta aattccaggc tttcgattgg ggtagcccgg cgcaaaacat gctgcactat 900
catcagccga ccccgccgta ctataatttg accgacatgc atgttccgat cgctgtttgg 960
aacggtggta atgatctgct ggcggacccg caagatgttg acctgttgct gagcaaattg 1020
ccgaacctga tctaccacaa gaaaattccg ccgtataatc atctggactt tatttgggct 1080
atggatgcgc cgcaggttat ctacaacgat attgttagca tgatgggcga gaacaataag 1140
taa                                                               1143

 
<210> 12
<211> 380
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Gastric lipase from Leptonychotes weddellii
 
<400> 12
Met Tyr Phe Gly Lys Leu His Pro Ile Ser Pro Glu Val Thr Met Asn 
1               5                   10                  15      
Ile Ser Gln Met Ile Thr Tyr Trp Gly Tyr Pro Ser Glu Glu Tyr Asp 
            20                  25                  30          
Val Val Thr Glu Asp Gly Tyr Ile Leu Gly Ile Asn Arg Ile Pro Tyr 
        35                  40                  45              
Gly Lys Lys Asn Ser Glu Asn Ile Gly Arg Arg Pro Val Ala Phe Leu 
    50                  55                  60                  
Gln His Gly Leu Leu Ala Ser Ala Thr Asn Trp Ile Ala Asn Leu Pro 
65                  70                  75                  80  
Asn Asn Ser Leu Ala Phe Ile Leu Ala Asp Ala Gly Tyr Asp Val Trp 
                85                  90                  95      
Leu Gly Asn Ser Arg Gly Asn Thr Trp Ala Arg Arg Asn Leu Tyr Phe 
            100                 105                 110         
Ser Pro Asp Ser Val Glu Phe Trp Ala Phe Ser Phe Asp Glu Met Ala 
        115                 120                 125             
Lys Tyr Asp Leu Pro Ala Thr Ile Asp Phe Ile Leu Lys Lys Thr Gly 
    130                 135                 140                 
Gln Asp Lys Leu His Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly 
145                 150                 155                 160 
Phe Ile Ala Phe Ser Thr Asn Pro Lys Leu Ala Lys Arg Ile Lys Thr 
                165                 170                 175     
Phe Tyr Ala Leu Ala Pro Val Ala Thr Val Lys Tyr Thr Lys Ser Leu 
            180                 185                 190         
Leu Asn Lys Leu Ala Leu Val Pro Ser Phe Leu Phe Lys Leu Ile Phe 
        195                 200                 205             
Gly Asp Lys Ile Phe Phe Pro His Gln Phe Phe Glu Gln Phe Leu Ala 
    210                 215                 220                 
Thr Glu Val Cys Ser Arg Glu Thr Val Ala Leu Leu Cys Ser Asn Ala 
225                 230                 235                 240 
Leu Phe Ile Ile Cys Gly Phe Asp Thr Lys Asn Leu Asn Val Ser Arg 
                245                 250                 255     
Leu Asp Val Tyr Leu Ser His Asn Pro Ala Gly Thr Ser Val Gln Asp 
            260                 265                 270         
Val Leu His Trp Ser Gln Ala Val Lys Ser Gly Lys Phe Gln Ala Phe 
        275                 280                 285             
Asp Trp Gly Ser Pro Ala Gln Asn Met Leu His Tyr His Gln Pro Thr 
    290                 295                 300                 
Pro Pro Tyr Tyr Asn Leu Thr Asp Met His Val Pro Ile Ala Val Trp 
305                 310                 315                 320 
Asn Gly Gly Asn Asp Leu Leu Ala Asp Pro Gln Asp Val Asp Leu Leu 
                325                 330                 335     
Leu Ser Lys Leu Pro Asn Leu Ile Tyr His Lys Lys Ile Pro Pro Tyr 
            340                 345                 350         
Asn His Leu Asp Phe Ile Trp Ala Met Asp Ala Pro Gln Val Ile Tyr 
        355                 360                 365             
Asn Asp Ile Val Ser Met Met Gly Glu Asn Asn Lys                 
    370                 375                 380                 

 
<210> 13
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 13
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaaa ggcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 14
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 14
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 15
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 15
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgagta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 16
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 16
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 17
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 17
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a                                           1161

 
<210> 18
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 18
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 19
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 19
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a                                           1161

 
<210> 20
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 20
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 21
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 21
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccattgttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 22
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 22
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Cys Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 23
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 23
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctattt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa                                               1158

 
<210> 24
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 24
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Ile Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 25
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 25
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aaggtattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 26
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 26
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 27
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 27
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcgcgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 28
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 28
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Ala Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 29
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 29
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtgtaaata a                                           1161

 
<210> 30
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 30
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Cys Lys                                                         
385                                                             

 
<210> 31
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 31
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cattgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 32
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 32
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 33
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 33
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa                                               1158

 
<210> 34
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 34
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 35
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 35
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac cgtggttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 36
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 36
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Val 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 37
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 37
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 38
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 38
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 39
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 39
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgtgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 40
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 40
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 41
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 41
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag tggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagacgtt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 42
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 42
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Thr Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 43
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 43
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatcggtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 44
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 44
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Arg Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 45
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 45
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgttcaaa                                               1158

 
<210> 46
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 46
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 47
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 47
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaaatgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 48
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 48
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Asn Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 49
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 49
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt gctaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 50
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 50
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Ala Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 51
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 51
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 52
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 52
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 53
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 53
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggcttgggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 54
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 54
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Trp Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 55
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 55
atgcatacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 56
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 56
Met His Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 57
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 57
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 58
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 58
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 59
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 59
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgactaaata a                                           1161

 
<210> 60
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 60
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Thr Lys                                                         
385                                                             

 
<210> 61
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 61
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 62
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 62
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 63
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 63
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aggaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 64
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 64
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Arg Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 65
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 65
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaaat atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa                                               1158

 
<210> 66
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 66
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 67
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 67
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtattg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 68
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 68
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 69
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 69
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgttgtccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 70
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 70
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Cys Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 71
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 71
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 72
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 72
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 73
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 73
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgcg 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 74
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 74
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Ala Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 75
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 75
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 76
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 76
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 77
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 77
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga ggcgtatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 78
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 78
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 79
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 79
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ttgaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 80
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 80
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Leu Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 81
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 81
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 82
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 82
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 83
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 83
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcggaaata a                                           1161

 
<210> 84
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 84
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Arg Lys                                                         
385                                                             

 
<210> 85
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 85
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgtta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 86
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 86
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 87
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 87
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctttct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 88
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 88
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Phe Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 89
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 89
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaacttggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 90
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 90
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Leu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 91
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 91
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtgcgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 92
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 92
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Ala Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 93
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 93
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaata a                                           1161

 
<210> 94
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 94
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 95
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 95
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagaggtt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttatccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 96
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 96
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Arg Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 97
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 97
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacctga ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a                                           1161

 
<210> 98
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 98
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Leu Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 99
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 99
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtgggccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 100
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 100
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gly Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 101
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 101
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaggag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 102
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 102
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Arg Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 103
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 103
atggagtcga gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 104
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 104
Met Glu Ser Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 105
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 105
atggagcgta gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 106
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 106
Met Glu Arg Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 107
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 107
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaaattggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 108
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 108
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Ile Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 109
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 109
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccggggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 110
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 110
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Arg Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 111
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 111
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctcgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 112
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 112
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Ser Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 113
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 113
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 114
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 114
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 115
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 115
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggttgtg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cgattattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 116
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 116
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Cys Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Ile Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 117
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 117
atggagacct ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 118
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 118
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 119
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 119
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 120
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 120
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 121
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 121
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtcttacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 122
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 122
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Ser Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 123
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 123
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acattcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 124
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 124
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Ile His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 125
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 125
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacaaattgt ttcgttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgtttgg tcatccgaat agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 126
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 126
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 127
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 127
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aagatgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa                                               1158

 
<210> 128
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 128
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Asp Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 129
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 129
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
agtgagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 130
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 130
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Ser Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 131
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 131
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaggagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 132
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 132
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 133
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 133
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg aggtgtacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 134
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 134
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Val Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 135
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 135
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcgtgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 136
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 136
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Val Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 137
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 137
atggaggcga gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 138
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 138
Met Glu Ala Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 139
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 139
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggttcgta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 140
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 140
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ser 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 141
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 141
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aaaggattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 142
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 142
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Arg Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 143
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 143
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a                                           1161

 
<210> 144
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 144
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 145
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 145
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
tttgagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 146
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 146
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Phe Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 147
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 147
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcttgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 148
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 148
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 149
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 149
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 150
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 150
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 151
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 151
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttttgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 152
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 152
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Phe Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 153
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 153
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatacg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 154
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 154
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Thr Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 155
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 155
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tcggttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 156
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 156
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Arg Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 157
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 157
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatag gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 158
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 158
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Arg Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 159
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 159
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 160
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 160
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 161
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 161
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtaatta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 162
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 162
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Asn 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 163
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 163
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt attaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 164
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 164
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Ile Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 165
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 165
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtacgccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 166
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 166
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Thr Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 167
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 167
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtcgtg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 168
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 168
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Arg Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 169
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 169
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaatttggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 170
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 170
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Phe Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 171
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 171
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgtat agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 172
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 172
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Tyr Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 173
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 173
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc actggcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 174
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 174
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Trp His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 175
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 175
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cggggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 176
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 176
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Gly Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 177
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 177
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agcagtacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 178
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 178
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Gln Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 179
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 179
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaacacg tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 180
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 180
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Thr Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 181
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 181
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gctttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 182
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 182
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Leu Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 183
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 183
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagcatg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 184
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 184
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln His Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 185
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 185
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cactgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 186
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 186
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Thr Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 187
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 187
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacctga ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a                                           1161

 
<210> 188
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 188
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Leu Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 189
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 189
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctatct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 190
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 190
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Tyr Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 191
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 191
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttggggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 192
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 192
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Trp Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 193
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 193
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtgttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 194
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 194
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Val Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 195
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 195
atgatgacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 196
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 196
Met Met Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 197
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 197
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
catgacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 198
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 198
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile His Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 199
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 199
atggagggga gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 200
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 200
Met Glu Gly Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 201
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 201
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tggataaata a                                           1161

 
<210> 202
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 202
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Asp Lys                                                         
385                                                             

 
<210> 203
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 203
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaata a                                           1161

 
<210> 204
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 204
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 205
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 205
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagggc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 206
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 206
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Gly His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 207
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 207
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatttat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcaggcag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgtttgg tcatccgact agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 208
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 208
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Ala Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Thr Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 209
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 209
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta tgagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 210
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 210
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Met 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 211
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 211
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gcggagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 212
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 212
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Ala Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 213
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 213
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcgc cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa                                               1158

 
<210> 214
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 214
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Ala Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 215
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 215
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcgctcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 216
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 216
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Ala Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 217
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 217
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
ttggagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 218
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 218
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Leu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 219
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 219
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 220
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 220
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Lys Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 221
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 221
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaaaagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 222
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 222
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Lys His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 223
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 223
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctggtgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 224
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 224
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Gly Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 225
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 225
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgggtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 226
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 226
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Gly Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 227
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 227
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggtt ggagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 228
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 228
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Trp 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 229
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 229
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggtc ggagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 230
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 230
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Arg 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 231
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 231
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg agcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 232
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 232
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Glu His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 233
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 233
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcgc cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa                                               1158

 
<210> 234
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 234
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Ala Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 235
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 235
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagctggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 236
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 236
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Ala Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 237
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 237
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agctgtcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 238
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 238
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Cys Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 239
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 239
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtatgta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 240
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 240
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Met Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 241
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 241
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtact 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 242
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 242
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Thr Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 243
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 243
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagtggtt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 244
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 244
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Trp Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 245
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 245
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgagggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgc ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgttcaaata a                                           1161

 
<210> 246
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 246
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 247
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 247
atggagacct atggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 248
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 248
Met Glu Thr Tyr Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 249
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 249
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agggttacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 250
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 250
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Gly Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 251
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 251
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta tggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtatgtaag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcctaaa                                               1158

 
<210> 252
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 252
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Val Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 253
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 253
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttggtg cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 254
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 254
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Val Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 255
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 255
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctacgcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 256
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 256
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Thr Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 257
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 257
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tactttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 258
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 258
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Thr Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 259
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 259
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcgtccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 260
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 260
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Arg Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 261
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 261
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtgcgtatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 262
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 262
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Ala Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 263
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 263
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agacttacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 264
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 264
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Thr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 265
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 265
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aagttgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 266
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 266
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Val Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 267
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 267
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcaggcag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 268
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 268
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Ala Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 269
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 269
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gcattatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 270
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 270
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu His Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 271
<211> 1161
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 271
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tggctaaata a                                           1161

 
<210> 272
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 272
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Ala Lys                                                         
385                                                             

 
<210> 273
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 273
atgactacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 274
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 274
Met Thr Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 275
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 275
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaacg gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 276
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 276
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Arg Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 277
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 277
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcataccac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 278
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 278
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Thr Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 279
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 279
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa atcaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 280
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 280
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Asn Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 281
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 281
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa ctcaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 282
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 282
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Thr Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 283
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 283
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacttattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 284
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 284
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 285
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 285
atggagacca tgggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 286
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 286
Met Glu Thr Met Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 287
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 287
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tgctagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggctatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 288
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 288
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Ala Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 289
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 289
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taatcagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 290
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 290
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Asn Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 291
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 291
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa atcaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 292
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 292
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Asn Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 293
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 293
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cgatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 294
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 294
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Asp Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 295
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 295
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggcctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttctgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 296
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 296
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Ser Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 297
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 297
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
agcgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa atcaggagag cttgctgtat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccag gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 298
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 298
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Ser Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Asn Gln Glu Ser Leu Leu Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 299
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 299
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
gttcaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 300
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 300
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Val Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 301
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 301
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctacgtt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 302
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 302
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Val Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 303
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 303
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttggcg cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 304
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 304
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Ala Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 305
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 305
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 306
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 306
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 307
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 307
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgttg atggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 308
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 308
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Asp Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 309
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 309
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgaggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 310
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 310
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Glu Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385                                                             

 
<210> 311
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 311
atggagacgt ggggtaacga ttatccgatt gctctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 312
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 312
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Ala Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 313
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 313
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 314
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 314
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 315
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 315
atggagtcgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 316
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 316
Met Glu Ser Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 317
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 317
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 318
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 318
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 319
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 319
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 320
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 320
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 321
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 321
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gccagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 322
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 322
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 323
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 323
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 324
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 324
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 325
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 325
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 326
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 326
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 327
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 327
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 328
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 328
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 329
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 329
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 330
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 330
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 331
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 331
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 332
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 332
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 333
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 333
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 334
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 334
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 335
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 335
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 336
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 336
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 337
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 337
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 338
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 338
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 339
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 339
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 340
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 340
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 341
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 341
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 342
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 342
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 343
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 343
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 344
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 344
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 345
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 345
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 346
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 346
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 347
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 347
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 348
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 348
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 349
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 349
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 350
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 350
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 351
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 351
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 352
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 352
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 353
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 353
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 354
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 354
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 355
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 355
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 356
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 356
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 357
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 357
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 358
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 358
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 359
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 359
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 360
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 360
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 361
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 361
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 362
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 362
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 363
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 363
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 364
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 364
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 365
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 365
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 366
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 366
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 367
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 367
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gccagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 368
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 368
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 369
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 369
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 370
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 370
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 371
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 371
atggagtcgt cgggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 372
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 372
Met Glu Ser Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 373
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 373
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 374
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 374
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 375
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 375
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 376
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 376
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 377
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 377
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 378
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 378
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 379
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 379
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtgaata cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcactggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccataagtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 380
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 380
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Glu 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Lys Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 381
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 381
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcttac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtgac 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 382
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 382
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 383
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 383
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gcttgtgagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 384
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 384
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 385
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 385
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt ttcgttcatc 540
aaggacctga ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 386
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 386
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Leu Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 387
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 387
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctgaggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 388
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 388
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Glu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 389
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 389
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt ttcgttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 390
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 390
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 391
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 391
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgtgagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 392
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 392
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 393
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 393
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 394
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 394
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 395
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 395
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tccgttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 396
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 396
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Pro Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 397
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 397
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctctggagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 398
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 398
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Leu Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 399
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 399
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt ttcgttcatc 540
aaggacttca ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccacgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 400
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 400
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 401
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 401
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatt cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcttcct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aagggattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 402
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 402
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Ser Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Phe Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 403
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 403
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtacgggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 404
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 404
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Thr Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 405
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 405
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 406
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 406
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 407
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 407
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgttttggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 408
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 408
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 409
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 409
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctgg atgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtatcg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catcgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 410
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 410
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Asp Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Ile Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Ile Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 411
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 411
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtatcta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagctttct gttcaccggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aagggattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aaaaccatgc acccgctgtt taccttgatc agcatgcaaa tgggtagcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 412
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 412
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Phe Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Met Gln Met Gly Ser Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 413
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 413
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 414
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 414
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 415
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 415
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gatgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 416
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 416
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 417
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 417
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 418
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 418
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 419
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 419
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtgattgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 420
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 420
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Asp Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 421
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 421
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 422
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 422
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 423
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 423
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 424
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 424
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 425
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 425
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccggg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 426
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 426
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 427
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 427
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaaaagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 428
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 428
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Lys Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 429
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 429
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagc ggtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 430
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 430
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Arg Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 431
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 431
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggggttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 432
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 432
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 433
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 433
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tacgagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 434
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 434
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Thr 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 435
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 435
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagctggg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 436
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 436
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Trp Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 437
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 437
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccaggg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 438
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 438
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 439
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 439
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 440
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 440
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 441
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 441
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 442
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 442
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 443
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 443
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcaacatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 444
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 444
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Asn Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 445
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 445
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 446
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 446
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 447
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 447
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tgcgagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 448
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 448
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Ala 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 449
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 449
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 450
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 450
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 451
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 451
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 452
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 452
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 453
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 453
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaagggt gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 454
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 454
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Gly Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 455
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 455
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcaacatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 456
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 456
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Asn Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 457
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 457
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 458
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 458
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 459
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 459
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 460
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 460
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 461
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 461
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 462
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 462
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 463
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 463
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaaccc ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 464
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 464
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr His Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 465
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 465
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 466
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 466
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 467
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 467
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgga tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 468
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 468
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 469
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 469
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac aggccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 470
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 470
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Arg Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 471
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 471
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acgcggacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 472
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 472
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 473
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 473
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ctatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 474
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 474
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Ala 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 475
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 475
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtaggat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 476
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 476
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Arg 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 477
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 477
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 478
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 478
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 479
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 479
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttgtgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 480
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 480
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Val Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 481
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 481
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttatgagcc tgccgaaa                                               1158

 
<210> 482
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 482
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val Met Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 483
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 483
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct ttgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 484
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 484
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Leu Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 485
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 485
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttatgagcc tgccgaaa                                               1158

 
<210> 486
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 486
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val Met Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 487
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 487
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatcttacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 488
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 488
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Leu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 489
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 489
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcagtg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 490
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 490
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Ser Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 491
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 491
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatacgacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 492
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 492
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Thr Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 493
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 493
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttctgcacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 494
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 494
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Leu His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 495
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 495
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaggg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 496
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 496
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Arg Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 497
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 497
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gcttgcgagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac accagattgt cagcttcatc 540
aaggacttta ttatccacct ggcttcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 498
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 498
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Cys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 499
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 499
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcgggg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 500
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 500
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Gly Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 501
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 501
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 502
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 502
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 503
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 503
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 504
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 504
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 505
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 505
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtctta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 506
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 506
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 507
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 507
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtacgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 508
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 508
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Thr 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 509
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 509
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtacta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 510
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 510
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Thr Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 511
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 511
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta taattactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 512
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 512
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Asn Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 513
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 513
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtagtat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 514
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 514
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ser 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 515
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 515
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag gcaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 516
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 516
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Arg Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 517
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 517
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cgagatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 518
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 518
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Glu Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 519
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 519
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 520
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 520
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 521
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 521
atggagacgt ggggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta tcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctagcgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcaacatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 522
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 522
Met Glu Thr Trp Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Asn Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 523
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 523
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtgat 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 524
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 524
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Asp Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 525
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 525
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtgt gcgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag aacagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 526
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 526
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Val Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 527
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 527
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 528
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 528
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 529
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 529
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtactg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 530
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 530
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 531
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 531
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 532
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 532
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 533
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 533
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 534
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 534
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 535
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 535
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 536
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 536
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 537
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 537
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacttgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 538
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 538
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 539
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 539
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 540
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 540
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 541
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 541
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt tcatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 542
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 542
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 543
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 543
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 544
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 544
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 545
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 545
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagccgcg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 546
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 546
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Arg Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 547
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 547
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aatatgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 548
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 548
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 549
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 549
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaaaaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 550
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 550
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Lys Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 551
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 551
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgttg atggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 552
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 552
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Asp Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 553
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 553
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttttgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 554
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 554
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 555
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 555
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 556
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 556
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 557
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 557
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcgtatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 558
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 558
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 559
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 559
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggggtatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 560
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 560
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 561
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 561
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 562
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 562
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 563
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 563
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 564
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 564
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 565
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 565
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 566
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 566
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 567
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 567
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 568
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 568
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 569
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 569
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgcattat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 570
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 570
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu His Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 571
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 571
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttgggacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 572
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 572
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Trp Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 573
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 573
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 574
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 574
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 575
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 575
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 576
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 576
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 577
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 577
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 578
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 578
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 579
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 579
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cgatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 580
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 580
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Asp Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 581
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 581
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagccgcg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 582
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 582
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Arg Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 583
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 583
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 584
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 584
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 585
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 585
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
caggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 586
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 586
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Gln Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 587
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 587
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 588
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 588
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 589
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 589
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 590
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 590
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 591
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 591
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt cccggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 592
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 592
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Pro Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 593
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 593
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ctgtgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 594
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 594
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Cys Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 595
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 595
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctggcgta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 596
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 596
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Ala Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 597
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 597
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc ttgacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 598
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 598
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 599
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 599
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctcatgct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 600
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 600
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala His Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 601
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 601
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgtcgta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 602
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 602
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Ser Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 603
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 603
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgcggta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 604
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 604
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Arg Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 605
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 605
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 606
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 606
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 607
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 607
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggttata aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaaaaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 608
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 608
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Tyr Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Lys Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 609
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 609
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 610
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 610
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 611
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 611
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggtt tgagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 612
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 612
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Leu 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 613
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 613
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttggtt cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 614
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 614
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Val Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 615
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 615
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatccgac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 616
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 616
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Pro Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 617
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 617
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acgcagacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 618
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 618
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Ala Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 619
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 619
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac cctggttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 620
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 620
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Leu 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 621
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 621
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 622
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 622
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 623
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 623
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 624
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 624
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 625
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 625
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agggcattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt catggcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaacg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 626
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 626
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe Met Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 627
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 627
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggcgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 628
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 628
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 629
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 629
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa ctcaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 630
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 630
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Thr Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 631
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 631
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac acctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggtgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcaaa tgggtcgcta cacccgtcag 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacaaggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 632
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 632
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 633
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 633
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 634
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 634
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 635
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 635
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 636
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 636
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 637
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 637
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 638
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 638
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 639
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 639
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 640
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 640
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 641
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 641
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 642
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 642
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 643
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 643
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 644
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 644
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 645
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 645
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 646
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 646
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 647
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 647
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 648
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 648
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 649
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 649
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 650
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 650
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 651
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 651
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aatacgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 652
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 652
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 653
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 653
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgcgaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 654
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 654
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 655
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 655
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 656
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 656
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 657
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 657
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 658
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 658
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 659
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 659
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 660
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 660
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 661
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 661
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 662
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 662
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 663
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 663
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 664
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 664
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 665
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 665
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aatacgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 666
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 666
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 667
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 667
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cacataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 668
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 668
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 669
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 669
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 670
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 670
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 671
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 671
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 672
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 672
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 673
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 673
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 674
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 674
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 675
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 675
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 676
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 676
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 677
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 677
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 678
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 678
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 679
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 679
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 680
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 680
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 681
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 681
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg tggccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 682
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 682
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Trp Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 683
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 683
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgcgaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 684
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 684
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 685
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 685
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aatacgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 686
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 686
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Tyr Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 687
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 687
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 688
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 688
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 689
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 689
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccctcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtacg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 690
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 690
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Pro Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Thr Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 691
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 691
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 692
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 692
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 693
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 693
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 694
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 694
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 695
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 695
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 696
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 696
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 697
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 697
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 698
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 698
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 699
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 699
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 700
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 700
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 701
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 701
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgcat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 702
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 702
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro His Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 703
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 703
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tcttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 704
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 704
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 705
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 705
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 706
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 706
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ile Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 707
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 707
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 708
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 708
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 709
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 709
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
cctaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 710
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 710
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 711
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 711
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 712
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 712
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 713
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 713
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aactggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 714
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 714
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Leu Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 715
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 715
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtacg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 716
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 716
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Thr Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 717
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 717
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgttg atggtaatta ttacccgatt 840
ggtaccatac acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgttt agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 718
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 718
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Asp Gly Asn Tyr Tyr Pro Ile Gly Thr Ile His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Phe Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 719
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 719
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccctcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggcagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg tggccgaacg acggtatcgt taatgttgtt 960
agcgcgaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 720
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 720
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Pro Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Trp Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 721
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 721
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 722
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 722
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 723
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 723
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccctcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 724
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 724
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Pro Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 725
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 725
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggcagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata gcggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 726
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 726
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Ser Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 727
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 727
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttctatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aaattgagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ctggtaatta ttacccgatt 840
aagaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaat agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gcattccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 728
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 728
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Ile Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Lys Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Ile Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 729
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 729
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 730
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 730
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 731
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 731
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 732
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 732
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 733
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 733
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctactcggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 734
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 734
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Ser Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 735
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 735
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccact 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 736
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 736
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Thr 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 737
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 737
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct gccgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc actttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 738
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 738
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Ala Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 739
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 739
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct gttgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 740
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 740
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Val Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 741
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 741
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 742
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 742
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 743
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 743
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacaccgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttcaac tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg tggccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 744
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 744
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Gln Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Trp Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 745
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 745
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta ccggcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 746
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 746
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Arg Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 747
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 747
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aagacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggttttcaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagctc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 748
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 748
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Phe Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 749
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 749
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 750
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 750
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 751
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 751
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaggaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 752
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 752
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 753
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 753
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 754
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 754
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 755
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 755
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 756
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 756
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 757
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 757
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 758
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 758
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 759
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 759
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 760
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 760
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 761
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 761
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 762
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 762
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 763
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 763
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 764
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 764
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 765
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 765
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 766
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 766
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 767
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 767
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 768
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 768
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 769
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 769
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 770
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 770
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 771
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 771
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 772
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 772
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 773
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 773
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 774
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 774
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 775
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 775
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggaggg cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 776
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 776
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Gly Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 777
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 777
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 778
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 778
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 779
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 779
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 780
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 780
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 781
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 781
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 782
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 782
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 783
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 783
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 784
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 784
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 785
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 785
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 786
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 786
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 787
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 787
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 788
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 788
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 789
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 789
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 790
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 790
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 791
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 791
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 792
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 792
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 793
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 793
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 794
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 794
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 795
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 795
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 796
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 796
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 797
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 797
atggagacca gcggtaacga ttatccgatt gttctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 798
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 798
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 799
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 799
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 800
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 800
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 801
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 801
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 802
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 802
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 803
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 803
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 804
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 804
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 805
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 805
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 806
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 806
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 807
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 807
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc gcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 808
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 808
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ala Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 809
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 809
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccggtgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 810
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 810
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Val Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 811
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 811
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 812
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 812
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 813
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 813
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat tcctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta tcatcactgc cgctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 814
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 814
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile Thr Ala Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 815
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 815
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 816
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 816
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 817
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 817
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 818
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 818
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 819
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 819
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 820
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 820
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 821
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 821
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 822
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 822
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 823
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 823
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 824
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 824
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 825
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 825
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta tcatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
ggtaccatgc acccgctgtt tacgttgatt agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtattgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 826
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 826
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 827
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 827
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 828
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 828
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 829
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 829
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 830
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 830
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 831
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 831
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc gcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 832
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 832
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Phe 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ala Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 833
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 833
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 834
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 834
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 835
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 835
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 836
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 836
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 837
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 837
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattct gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 838
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 838
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Leu Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 839
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 839
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cctgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
tcgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 840
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 840
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Phe 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Ser Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 841
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 841
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact atgttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccggtgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 842
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 842
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Tyr Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Val Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 843
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 843
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 844
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 844
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 845
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 845
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 846
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 846
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 847
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 847
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tacttaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 848
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 848
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Thr Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 849
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 849
atggagacca gcggtaacga ttatccgatt atcctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 850
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 850
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Ile Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 851
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 851
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtctggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 852
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 852
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 853
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 853
atggagacca gcggtaacga ttatccgatt atcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacgc ctacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattgt gagcttcgtc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ctatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 854
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 854
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Ile Leu Val His Gly Phe 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Ala Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Val Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 855
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 855
atggagacca gcggtaacga ttatccgatt atcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagtaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca gtatgacctg 720
tcgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgct gaccttgatc tcgctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 856
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 856
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Ile Leu Val His Gly Phe 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Ser Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 857
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 857
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 858
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 858
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 859
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 859
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccgaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 860
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 860
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 861
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 861
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ctggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 862
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 862
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 863
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 863
atggagacca gcggtaacga ttatccgatt gtcctggttc acggtttcgg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tactaccttt gcggatcaac gtctgattct gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ctatgacctg 720
acgaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 864
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 864
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Phe 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Leu Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 865
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 865
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctgc tggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta tcatccacct ggctgcggct gcgggtcaaa aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccctgg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ggtaccatgc acccgctgtt tacgttgatt agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtattgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 866
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 866
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Leu Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 867
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 867
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 868
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 868
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 869
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 869
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 870
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 870
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 871
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 871
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 872
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 872
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 873
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 873
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccaaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 874
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 874
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 875
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 875
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tacgaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
ccaaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 876
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 876
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Thr Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Pro Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 877
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 877
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 878
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 878
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 879
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 879
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg ttcgaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagtcgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 880
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 880
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Ser Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 881
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 881
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg ttcgaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta tctgccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 882
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 882
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Leu Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

 
<210> 883
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 883
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt gagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcca atatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 884
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 884
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Gln Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385                                                             

<210> 885
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 885
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag cgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 886
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 886
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Ala Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 887
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 887
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat gtctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 888
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 888
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Cys Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 889
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 889
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 890
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 890
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Asp Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 891
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 891
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag agctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 892
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 892
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Glu Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 893
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 893
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 894
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 894
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Phe Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 895
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 895
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag gtctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 896
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 896
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Gly Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 897
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 897
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 898
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 898
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu His Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 899
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 899
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 900
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 900
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Ile Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 901
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 901
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa agctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 902
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 902
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Lys Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 903
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 903
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac ttctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 904
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 904
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Leu Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 905
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 905
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 906
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 906
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Asn Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 907
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 907
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac ctctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 908
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 908
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Pro Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 909
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 909
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaac aactgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 910
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 910
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Gln Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 911
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 911
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa ggctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 912
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 912
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Arg Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 913
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 913
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa gtctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 914
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 914
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Ser Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 915
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 915
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa cgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 916
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 916
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Thr Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 917
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 917
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaag tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 918
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 918
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Val Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 919
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 919
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat ggctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 920
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 920
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Trp Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 921
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 921
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaat atctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 922
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 922
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Tyr Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 923
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 923
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggcttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 924
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 924
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ala Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 925
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 925
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gtgttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 926
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 926
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Cys Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 927
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 927
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggattatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 928
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 928
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Asp Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 929
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 929
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggagtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 930
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 930
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Glu Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 931
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 931
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gttttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 932
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 932
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Phe Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 933
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 933
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gcactatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 934
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 934
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu His Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 935
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 935
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gatttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 936
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 936
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ile Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 937
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 937
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gaagtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 938
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 938
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Lys Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 939
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 939
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gctgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 940
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 940
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Leu Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 941
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 941
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gatgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 942
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 942
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Met Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 943
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 943
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gaactatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 944
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 944
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Asn Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 945
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 945
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gccgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 946
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 946
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Pro Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 947
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 947
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gcagtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 948
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 948
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gln Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 949
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 949
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gaggtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 950
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 950
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Arg Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 951
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 951
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gagttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 952
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 952
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Ser Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 953
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 953
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gacttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 954
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 954
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Thr Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 955
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 955
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga ggtgtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 956
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 956
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Val Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 957
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 957
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gtggtatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 958
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 958
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Trp Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 959
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 959
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gtattatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 960
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 960
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Tyr Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 961
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 961
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgcggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 962
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 962
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Ala Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 963
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 963
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactgtgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 964
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 964
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Cys Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 965
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 965
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgacgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 966
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 966
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Asp Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 967
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 967
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgaggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 968
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 968
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Glu Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 969
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 969
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactttgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 970
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 970
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Phe Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 971
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 971
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacggggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 972
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 972
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Gly Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 973
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 973
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctaccacgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 974
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 974
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
His Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 975
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 975
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacatcgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 976
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 976
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Ile Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 977
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 977
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacaaggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 978
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 978
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Lys Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 979
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 979
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacctggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 980
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 980
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Leu Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 981
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 981
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacatggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 982
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 982
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Met Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 983
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 983
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacaatgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 984
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 984
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Asn Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 985
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 985
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacccggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 986
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 986
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Pro Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 987
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 987
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctaccaggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 988
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 988
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Gln Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 989
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 989
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacagggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 990
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 990
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Arg Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 991
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 991
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactcggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 992
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 992
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Ser Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 993
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 993
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacgtggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 994
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 994
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Val Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 995
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 995
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactgggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 996
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 996
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Trp Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 997
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 997
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctactatgct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 998
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 998
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Tyr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 999
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 999
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgct ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1000
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1000
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Ala Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1001
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1001
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttgt ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1002
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1002
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Cys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1003
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1003
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgac ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1004
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1004
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Asp Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1005
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1005
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1006
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1006
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Glu Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1007
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1007
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattttt ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1008
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1008
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Phe Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1009
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1009
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattggg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1010
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1010
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Gly Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1011
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1011
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattatc ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1012
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1012
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Ile Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1013
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1013
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1014
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1014
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Lys Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1015
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1015
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattctg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1016
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1016
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Leu Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1017
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1017
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattatg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1018
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1018
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Met Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1019
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1019
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattaat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1020
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1020
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Asn Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1021
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1021
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattccg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1022
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1022
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Pro Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1023
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1023
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcag ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1024
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1024
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Gln Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1025
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1025
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcgg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1026
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1026
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Arg Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1027
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1027
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttcg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1028
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1028
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Ser Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1029
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1029
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattacg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1030
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1030
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Thr Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1031
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1031
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattgtg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1032
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1032
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Val Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1033
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1033
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttgg ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1034
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1034
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Trp Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1035
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1035
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacatttat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1036
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1036
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile Tyr Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1037
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1037
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgcgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1038
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1038
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Ala Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1039
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1039
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctgtggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1040
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1040
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Cys Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1041
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1041
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgatggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1042
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1042
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Asp Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1043
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1043
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctttggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1044
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1044
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Phe Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1045
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1045
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgggggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1046
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1046
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Gly Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1047
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1047
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccacggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1048
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1048
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe His Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1049
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1049
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcattggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1050
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1050
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Ile Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1051
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1051
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaagggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1052
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1052
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Lys Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1053
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1053
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcttgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1054
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1054
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Leu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1055
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1055
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcatgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1056
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1056
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Met Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1057
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1057
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcaacggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1058
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1058
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Asn Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1059
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1059
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccctggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1060
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1060
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Pro Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1061
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1061
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccagggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1062
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1062
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Gln Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1063
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1063
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttccgtggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1064
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1064
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Arg Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1065
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1065
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctcgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1066
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1066
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Ser Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1067
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1067
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcacgggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1068
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1068
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Thr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1069
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1069
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgtaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1070
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1070
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Val Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1071
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1071
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctggggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1072
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1072
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Trp Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1073
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1073
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttctatggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1074
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1074
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Tyr Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1075
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1075
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gcggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1076
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1076
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ala Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1077
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1077
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tgtgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1078
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1078
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Cys Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1079
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1079
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gatgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1080
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1080
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Asp Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1081
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1081
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gaggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1082
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1082
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Glu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1083
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1083
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttcgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1084
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1084
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Phe Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1085
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1085
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ggtgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1086
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1086
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Gly Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1087
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1087
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
catgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1088
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1088
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
His Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1089
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1089
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
aaggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1090
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1090
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Lys Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1091
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1091
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ttggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1092
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1092
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Leu Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1093
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1093
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
atggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1094
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1094
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Met Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1095
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1095
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
aatgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1096
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1096
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Asn Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1097
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1097
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
ccggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1098
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1098
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Pro Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1099
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1099
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
caggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1100
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1100
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Gln Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1101
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1101
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
cgggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1102
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1102
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Arg Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1103
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1103
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tctgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1104
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1104
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ser Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1105
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1105
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
actgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1106
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1106
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Thr Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1107
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1107
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
gtggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1108
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1108
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Val Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1109
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1109
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tgggctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1110
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1110
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Trp Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1111
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1111
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
tatgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1112
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1112
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Tyr Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1113
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1113
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag ctctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1114
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1114
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Ala Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1115
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1115
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1116
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1116
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Cys Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1117
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1117
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1118
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1118
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Asp Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1119
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1119
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1120
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1120
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Glu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1121
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1121
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat ttctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1122
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1122
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Phe Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1123
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1123
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1124
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1124
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gly Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1125
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1125
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1126
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1126
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln His Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1127
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1127
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa ttctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1128
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1128
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Ile Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1129
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1129
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1130
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1130
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Lys Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1131
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1131
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac tgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1132
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1132
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Leu Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1133
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1133
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa tgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1134
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1134
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Met Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1135
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1135
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1136
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1136
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Asn Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1137
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1137
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac ctctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1138
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1138
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Pro Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1139
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1139
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac agctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1140
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1140
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Gln Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1141
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1141
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1142
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1142
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Ser Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1143
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1143
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaaa cgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1144
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1144
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Thr Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1145
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1145
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaag tgctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1146
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1146
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Val Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1147
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1147
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat ggctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1148
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1148
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Trp Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1149
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1149
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaat atctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1150
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1150
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Tyr Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1151
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1151
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgctattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1152
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1152
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Ala Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1153
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1153
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttgtattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1154
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1154
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Cys Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1155
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1155
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1156
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1156
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Asp Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1157
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1157
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1158
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1158
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Glu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1159
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1159
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttttattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1160
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1160
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Phe Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1161
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1161
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtggtattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1162
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1162
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gly Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1163
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1163
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1164
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1164
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg His Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1165
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1165
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtattattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1166
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1166
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Ile Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1167
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1167
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtaagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1168
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1168
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Lys Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1169
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1169
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtatgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1170
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1170
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Met Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1171
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1171
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtaatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1172
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1172
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Asn Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1173
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1173
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcctattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1174
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1174
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Pro Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1175
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1175
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcagattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1176
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1176
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Gln Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1177
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1177
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtcggattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1178
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1178
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Arg Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1179
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1179
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttcgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1180
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1180
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Ser Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1181
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1181
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtactattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1182
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1182
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Thr Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1183
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1183
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtgtgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1184
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1184
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Val Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1185
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1185
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttggattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1186
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1186
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Trp Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1187
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1187
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gttatattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1188
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1188
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Tyr Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1189
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1189
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttgtgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1190
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1190
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Cys Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1191
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1191
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgatgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1192
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1192
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Asp Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1193
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1193
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgaggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1194
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1194
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Glu Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1195
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1195
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttttgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1196
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1196
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Phe Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1197
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1197
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctggggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1198
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1198
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Gly Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1199
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1199
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctcatgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1200
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1200
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala His Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1201
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1201
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctatcgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1202
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1202
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ile Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1203
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1203
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctaaggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1204
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1204
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Lys Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1205
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1205
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctctggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1206
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1206
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Leu Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1207
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1207
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctatggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1208
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1208
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Met Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1209
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1209
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctaacgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1210
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1210
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Asn Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1211
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1211
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctccggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1212
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1212
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Pro Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1213
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1213
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctcaggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1214
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1214
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Gln Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1215
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1215
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctagggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1216
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1216
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Arg Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1217
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1217
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctagtgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1218
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1218
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ser Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1219
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1219
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctacggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1220
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1220
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Thr Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1221
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1221
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgtggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1222
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1222
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Val Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1223
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1223
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttgggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1224
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1224
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Trp Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1225
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1225
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggcttatgct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1226
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1226
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Tyr Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1227
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1227
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgcga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1228
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1228
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Ala Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1229
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1229
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggttgta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1230
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1230
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Cys Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1231
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1231
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1232
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1232
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Asp Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1233
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1233
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1234
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1234
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Glu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1235
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1235
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtttta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1236
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1236
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Phe Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1237
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1237
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtggta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1238
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1238
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gly Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1239
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1239
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1240
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1240
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
His Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1241
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1241
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtatta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1242
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1242
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Ile Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1243
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1243
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtaaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1244
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1244
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Lys Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1245
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1245
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtatga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1246
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1246
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Met Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1247
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1247
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtaata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1248
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1248
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Asn Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1249
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1249
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtccga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1250
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1250
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Pro Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1251
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1251
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcaga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1252
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1252
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Gln Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1253
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1253
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtcgga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1254
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1254
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Arg Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1255
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1255
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtagta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1256
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1256
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Ser Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1257
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1257
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtacta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1258
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1258
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Thr Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1259
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1259
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtgtta aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1260
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1260
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Val Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1261
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1261
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggttgga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1262
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1262
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Trp Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1263
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1263
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggttata aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1264
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1264
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Tyr Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1265
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1265
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgcgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1266
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1266
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Ala Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1267
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1267
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttgtaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1268
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1268
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Cys Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1269
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1269
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgataccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1270
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1270
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Asp Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1271
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1271
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgagaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1272
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1272
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Glu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1273
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1273
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttttaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1274
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1274
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Phe Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1275
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1275
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgggaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1276
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1276
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Gly Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1277
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1277
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttcacaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1278
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1278
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
His Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1279
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1279
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttattaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1280
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1280
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Ile Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1281
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1281
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttaaaaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1282
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1282
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Lys Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1283
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1283
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttatgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1284
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1284
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Met Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1285
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1285
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttaacaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1286
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1286
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Asn Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1287
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1287
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttccgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1288
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1288
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Pro Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1289
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1289
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttcaaaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1290
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1290
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Gln Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1291
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1291
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttaggaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1292
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1292
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Arg Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1293
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1293
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttcgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1294
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1294
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Ser Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1295
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1295
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttacgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1296
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1296
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Thr Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1297
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1297
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttgtgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1298
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1298
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Val Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1299
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1299
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttggaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1300
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1300
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Trp Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1301
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1301
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg tttataccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1302
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1302
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Tyr Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1303
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1303
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gcgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1304
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1304
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Ala Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1305
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1305
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tgtagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1306
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1306
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Cys Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1307
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1307
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gatagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1308
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1308
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Asp Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1309
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1309
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tttagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1310
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1310
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Phe Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1311
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1311
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gggagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1312
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1312
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Gly Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1313
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1313
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg cacagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1314
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1314
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp His Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1315
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1315
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg attagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1316
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1316
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Ile Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1317
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1317
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg aaaagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1318
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1318
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Lys Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1319
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1319
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg ctgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1320
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1320
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Leu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1321
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1321
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg atgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1322
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1322
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Met Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1323
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1323
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg aatagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1324
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1324
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Asn Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1325
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1325
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg cctagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1326
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1326
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Pro Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1327
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1327
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg cagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1328
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1328
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Gln Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1329
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1329
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg aggagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1330
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1330
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Arg Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1331
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1331
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg agtagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1332
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1332
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Ser Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1333
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1333
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg acgagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1334
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1334
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Thr Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1335
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1335
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gttagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1336
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1336
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Val Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1337
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1337
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tggagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1338
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1338
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Trp Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1339
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1339
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg tatagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1340
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1340
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Tyr Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1341
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1341
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgctg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1342
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1342
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Ala Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1343
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1343
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacctgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1344
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1344
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Cys Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1345
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1345
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgatg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1346
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1346
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Asp Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1347
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1347
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgagg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1348
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1348
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Glu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1349
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1349
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttcg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1350
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1350
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Phe Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1351
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1351
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgggg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1352
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1352
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Gly Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1353
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1353
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccatg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1354
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1354
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr His Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1355
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1355
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccattg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1356
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1356
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Ile Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1357
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1357
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccaagg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1358
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1358
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Lys Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1359
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1359
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccttgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1360
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1360
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Leu Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1361
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1361
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccatgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1362
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1362
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Met Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1363
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1363
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccaacg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1364
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1364
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Asn Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1365
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1365
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccccgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1366
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1366
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Pro Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1367
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1367
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccagg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1368
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1368
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Gln Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1369
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1369
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccagtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1370
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1370
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Ser Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1371
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1371
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccacgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1372
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1372
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Thr Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1373
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1373
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accaccgtgg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1374
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1374
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Val Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1375
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1375
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacctggg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1376
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1376
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Trp Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1377
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1377
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacctacg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1378
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1378
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Tyr Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1379
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1379
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggcgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1380
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1380
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Ala Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1381
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1381
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtgcaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1382
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1382
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Cys Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1383
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1383
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1384
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1384
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Asp Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1385
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1385
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggagaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1386
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1386
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Glu Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1387
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1387
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtttaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1388
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1388
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Phe Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1389
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1389
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggggaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1390
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1390
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Gly Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1391
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1391
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1392
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1392
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp His Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1393
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1393
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggattaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1394
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1394
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Ile Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1395
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1395
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggaaaaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1396
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1396
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Lys Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1397
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1397
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcttaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1398
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1398
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Leu Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1399
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1399
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggatgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1400
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1400
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Met Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1401
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1401
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggaataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1402
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1402
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Asn Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1403
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1403
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggccgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1404
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1404
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Pro Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1405
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1405
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcagaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1406
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1406
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Gln Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1407
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1407
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggcggaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1408
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1408
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Arg Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1409
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1409
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtctaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1410
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1410
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Ser Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1411
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1411
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggactaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1412
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1412
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Thr Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1413
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1413
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtggaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1414
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1414
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Trp Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1415
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1415
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tggtataaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1416
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1416
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Tyr Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1417
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1417
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gcggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1418
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1418
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Ala Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1419
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1419
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tgtgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1420
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1420
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Cys Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1421
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1421
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gaggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1422
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1422
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Glu Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1423
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1423
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tttgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1424
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1424
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Phe Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1425
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1425
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ggggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1426
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1426
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gly Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1427
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1427
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt catgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1428
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1428
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg His Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1429
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1429
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt attgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1430
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1430
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Ile Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1431
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1431
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt aaggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1432
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1432
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Lys Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1433
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1433
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ttggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1434
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1434
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Leu Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1435
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1435
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt atggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1436
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1436
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Met Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1437
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1437
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt aatgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1438
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1438
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asn Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1439
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1439
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt ccggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1440
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1440
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Pro Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1441
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1441
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt caggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1442
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1442
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Gln Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1443
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1443
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt cgggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1444
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1444
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Arg Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1445
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1445
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tcggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1446
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1446
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Ser Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1447
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1447
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt acggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1448
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1448
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Thr Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1449
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1449
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gttgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1450
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1450
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Val Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1451
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1451
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tgggttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1452
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1452
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Trp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1453
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1453
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt tatgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1454
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1454
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Tyr Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1455
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1455
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctggc gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1456
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1456
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Ala Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1457
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1457
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtg caccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1458
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1458
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Cys Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1459
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1459
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgga taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1460
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1460
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Asp Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1461
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1461
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgga gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1462
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1462
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Glu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1463
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1463
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1464
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1464
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Phe Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1465
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1465
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctggg gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1466
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1466
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Gly Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1467
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1467
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgca taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1468
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1468
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu His Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1469
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1469
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgat caccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1470
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1470
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Ile Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1471
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1471
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgaa gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1472
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1472
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Lys Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1473
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1473
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgat gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1474
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1474
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Met Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1475
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1475
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgaa taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1476
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1476
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Asn Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1477
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1477
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgcc gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1478
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1478
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Pro Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1479
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1479
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgca gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1480
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1480
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Gln Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1481
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1481
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgag gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1482
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1482
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Arg Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1483
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1483
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgag taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1484
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1484
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Ser Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1485
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1485
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgac gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1486
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1486
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Thr Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1487
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1487
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctggt taccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1488
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1488
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Val Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1489
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1489
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgtg gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1490
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1490
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Trp Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1491
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1491
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgta caccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1492
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1492
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Tyr Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1493
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1493
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgcgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1494
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1494
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Ala Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1495
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1495
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctgtcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1496
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1496
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Cys Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1497
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1497
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1498
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1498
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Asp Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1499
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1499
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgagcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1500
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1500
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Glu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1501
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1501
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctttcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1502
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1502
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Phe Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1503
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1503
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgggcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1504
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1504
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Gly Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1505
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1505
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agccatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1506
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1506
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser His Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1507
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1507
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcattcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1508
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1508
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Ile Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1509
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1509
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcaagcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1510
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1510
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Lys Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1511
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1511
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcatgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1512
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1512
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Met Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1513
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1513
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcaatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1514
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1514
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Asn Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1515
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1515
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcccacgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1516
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1516
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Pro Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1517
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1517
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agccagcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1518
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1518
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Gln Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1519
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1519
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agccggcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1520
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1520
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Arg Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1521
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1521
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctctcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1522
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1522
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Ser Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1523
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1523
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcacgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1524
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1524
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Thr Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1525
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1525
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcgtgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1526
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1526
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Val Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1527
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1527
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctggcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1528
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1528
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Trp Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1529
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1529
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agctatcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1530
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1530
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Tyr Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1531
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1531
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggcta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1532
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1532
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Ala Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1533
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1533
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgtgta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1534
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1534
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Cys Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1535
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1535
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1536
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1536
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Asp Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1537
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1537
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggaga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1538
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1538
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Glu Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1539
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1539
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgttta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1540
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1540
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Phe Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1541
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1541
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgggga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1542
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1542
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Gly Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1543
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1543
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1544
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1544
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu His Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1545
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1545
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgatta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1546
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1546
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Ile Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1547
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1547
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgaaga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1548
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1548
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Lys Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1549
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1549
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgctga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1550
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1550
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Leu Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1551
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1551
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgatga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1552
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1552
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Met Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1553
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1553
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgaata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1554
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1554
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Asn Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1555
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1555
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgccga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1556
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1556
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Pro Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1557
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1557
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcaga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1558
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1558
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Gln Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1559
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1559
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgagta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1560
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1560
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Ser Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1561
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1561
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgacga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1562
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1562
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Thr Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1563
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1563
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctggtta tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1564
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1564
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Val Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1565
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1565
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgtgga tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1566
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1566
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Trp Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1567
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1567
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgtata tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1568
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1568
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Tyr Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1569
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1569
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgcgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1570
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1570
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Ala Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1571
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1571
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggttgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1572
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1572
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Cys Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1573
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1573
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1574
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1574
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Asp Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1575
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1575
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgagta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1576
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1576
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Glu Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1577
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1577
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtttcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1578
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1578
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Phe Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1579
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1579
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgggta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1580
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1580
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Gly Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1581
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1581
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1582
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1582
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly His Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1583
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1583
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtatcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1584
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1584
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Ile Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1585
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1585
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtaagta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1586
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1586
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Lys Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1587
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1587
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcttta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1588
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1588
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Leu Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1589
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1589
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtatgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1590
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1590
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Met Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1591
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1591
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtaacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1592
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1592
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Asn Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1593
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1593
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtccgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1594
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1594
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Pro Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1595
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1595
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcagta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1596
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1596
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Gln Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1597
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1597
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtagcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1598
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1598
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Ser Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1599
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1599
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtacgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1600
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1600
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Thr Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1601
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1601
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtgtgta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1602
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1602
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Val Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1603
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1603
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggttggta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1604
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1604
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Trp Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1605
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1605
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggttacta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1606
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1606
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Tyr Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1607
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1607
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagcgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1608
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1608
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ala Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1609
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1609
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatgtc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1610
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1610
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Cys Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1611
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1611
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1612
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1612
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Asp Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1613
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1613
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagagc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1614
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1614
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Glu Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1615
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1615
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatttc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1616
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1616
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Phe Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1617
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1617
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaggtc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1618
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1618
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Gly Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1619
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1619
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1620
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1620
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro His Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1621
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1621
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaattc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1622
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1622
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ile Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1623
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1623
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaaagc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1624
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1624
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Lys Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1625
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1625
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccattgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1626
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1626
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Leu Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1627
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1627
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaatgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1628
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1628
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Met Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1629
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1629
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaaatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1630
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1630
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Asn Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1631
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1631
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccacagc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1632
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1632
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Gln Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1633
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1633
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaaggc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1634
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1634
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Arg Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1635
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1635
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatcgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1636
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1636
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Ser Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1637
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1637
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaacgc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1638
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1638
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Thr Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1639
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1639
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccagttc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1640
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1640
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Val Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1641
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1641
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatggc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1642
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1642
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Trp Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1643
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1643
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccatatc cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1644
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1644
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Tyr Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1645
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1645
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg cgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1646
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1646
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Ala Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1647
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1647
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact gtggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1648
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1648
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Cys Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1649
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1649
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1650
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1650
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1651
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1651
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg agggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1652
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1652
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Glu Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1653
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1653
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact ttggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1654
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1654
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Phe Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1655
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1655
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg ggggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1656
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1656
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Gly Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1657
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1657
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1658
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1658
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr His Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1659
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1659
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ttggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1660
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1660
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Ile Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1661
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1661
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca agggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1662
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1662
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Lys Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1663
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1663
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc tgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1664
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1664
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Leu Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1665
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1665
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca tgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1666
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1666
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Met Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1667
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1667
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1668
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1668
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Asn Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1669
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1669
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1670
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1670
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Pro Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1671
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1671
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc agggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1672
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1672
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Gln Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1673
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1673
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacc ggggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1674
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1674
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Arg Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1675
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1675
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact cgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1676
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1676
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Ser Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1677
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1677
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtacg tgggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1678
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1678
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Val Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1679
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1679
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact ggggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1680
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1680
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Trp Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1681
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1681
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtact atggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1682
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1682
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Tyr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1683
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1683
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg cttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1684
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1684
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ala Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1685
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1685
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt gctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1686
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1686
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Cys Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1687
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1687
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1688
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1688
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Asp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1689
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1689
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg agtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1690
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1690
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Glu Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1691
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1691
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1692
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1692
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Phe Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1693
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1693
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1694
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1694
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Gly Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1695
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1695
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata tctttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1696
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1696
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ile Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1697
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1697
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata agtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1698
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1698
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Lys Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1699
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1699
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc tttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1700
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1700
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Leu Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1701
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1701
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata tgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1702
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1702
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Met Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1703
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1703
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1704
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1704
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Asn Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1705
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1705
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1706
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1706
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Pro Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1707
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1707
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc agtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1708
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1708
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Gln Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1709
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1709
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc gttttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1710
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1710
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Arg Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1711
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1711
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1712
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1712
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Ser Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1713
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1713
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaata cgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1714
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1714
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Thr Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1715
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1715
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatg tgtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1716
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1716
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Val Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1717
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1717
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt ggtttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1718
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1718
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Trp Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1719
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1719
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatt attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1720
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1720
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn Tyr Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1721
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1721
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgcgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1722
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1722
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Ala Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1723
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1723
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttgtt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1724
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1724
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Cys Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1725
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1725
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1726
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1726
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Asp Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1727
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1727
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgagt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1728
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1728
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Glu Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1729
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1729
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgggt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1730
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1730
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Gly Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1731
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1731
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtcatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1732
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1732
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly His Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1733
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1733
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtattt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1734
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1734
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Ile Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1735
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1735
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtaagt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1736
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1736
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Lys Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1737
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1737
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtttgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1738
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1738
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Leu Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1739
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1739
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtatgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1740
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1740
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Met Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1741
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1741
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtaatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1742
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1742
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Asn Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1743
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1743
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtccgt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1744
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1744
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Pro Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1745
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1745
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtcaat acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1746
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1746
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Gln Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1747
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1747
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtaggt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1748
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1748
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Arg Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1749
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1749
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttctt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1750
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1750
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Ser Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1751
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1751
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtactt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1752
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1752
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Thr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1753
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1753
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggtgttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1754
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1754
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Val Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1755
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1755
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttggt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1756
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1756
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Trp Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1757
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1757
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttatt acaacgacgt tgctaatcgt 1140
gttcatagcc tgccgaaa                                               1158

 
<210> 1758
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1758
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Tyr Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Pro Lys                                                         
385     

 
<210> 1759
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1759
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggcgaaa                                               1158

 
<210> 1760
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1760
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Ala Lys                                                         
385     

 
<210> 1761
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1761
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtgtaaa                                               1158

 
<210> 1762
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1762
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Cys Lys                                                         
385     

 
<210> 1763
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1763
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggataaa                                               1158

 
<210> 1764
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1764
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Asp Lys                                                         
385     

 
<210> 1765
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1765
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggagaaa                                               1158

 
<210> 1766
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1766
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Glu Lys                                                         
385     

 
<210> 1767
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1767
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtttaaa                                               1158

 
<210> 1768
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1768
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Phe Lys                                                         
385     

 
<210> 1769
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1769
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgggtaaa                                               1158

 
<210> 1770
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1770
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Gly Lys                                                         
385     

 
<210> 1771
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1771
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcataaa                                               1158

 
<210> 1772
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1772
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
His Lys                                                         
385     

 
<210> 1773
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1773
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgattaaa                                               1158

 
<210> 1774
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1774
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Ile Lys                                                         
385     

 
<210> 1775
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1775
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgaagaaa                                               1158

 
<210> 1776
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1776
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Lys Lys                                                         
385     

 
<210> 1777
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1777
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgttgaaa                                               1158

 
<210> 1778
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1778
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Leu Lys                                                         
385     

 
<210> 1779
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1779
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgatgaaa                                               1158

 
<210> 1780
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1780
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Met Lys                                                         
385     

 
<210> 1781
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1781
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgaataaa                                               1158

 
<210> 1782
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1782
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Asn Lys                                                         
385     

 
<210> 1783
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1783
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcagaaa                                               1158

 
<210> 1784
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1784
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Gln Lys                                                         
385     

 
<210> 1785
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1785
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgcgtaaa                                               1158

 
<210> 1786
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1786
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Arg Lys                                                         
385     

 
<210> 1787
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1787
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtcgaaa                                               1158

 
<210> 1788
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1788
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Ser Lys                                                         
385     

 
<210> 1789
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1789
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgacgaaa                                               1158

 
<210> 1790
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1790
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Thr Lys                                                         
385     

 
<210> 1791
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1791
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tggtgaaa                                               1158

 
<210> 1792
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1792
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Val Lys                                                         
385     

 
<210> 1793
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1793
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtggaaa                                               1158

 
<210> 1794
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1794
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Trp Lys                                                         
385     

 
<210> 1795
<211> 1158
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1795
atggagacca gcggtaacga ttatccgatt gttctggttc acggtttggg tggttggggt 60
aaaggcgaaa tgctgggtta ccgttactgg ggtggtctga aggacatcga gggttatttg 120
aaccaaaccg gtcatcgtac ctacacggct accgttggtc cggttagcag caattgggat 180
cgtgcggttg aattgtacta ttacattcat ggtggtaccg ttgactacgg tgctgcgcac 240
gctaaagagc acggtcatgc gcgtttcggt cgtacctatc cgggtattta cggtcaatgg 300
gatgaaacca ataagattca cctgatcggt catagcatgg gtggtcagac cagccgtatg 360
ttggttgagc tgttgaagag cggtagccaa aaagaacagg agtattacag ccaacatccg 420
gaagagggta ttagcccgct gttcgaaggt ggtaaaaact gggttcacag cgttaccagc 480
attgctaccc cgcataatgg tagcaccttt gcggatcaac gtctgattgt tagcttcatc 540
aaggacttta ttatccacct ggctgcggct gcgggtctga aacaggagag cttgatctat 600
gatttcaagc tggaccaatg gggtttgaaa cgtcagccgg gtgagagctt ccatgcgtac 660
atgaaccgtg ttctgaccag cccgatttgg gagagcaatg atatcagcgc ttatgacctg 720
accacccgtg gtgcgcaaga gctgaaccag tgggtgaaga cctacccgga cgtttattac 780
ttgagctata ccggtaacgc tagctaccgt gacgttgtta ccggtaatta ttacccgatt 840
gggaccatgc acccgctgct gaccttgatc agcctgcgca tgggtcgcta cacccgtcaa 900
agcccaccac cggttattga tcgtagctgg ctgccgaacg acggtatcgt taatgttgtt 960
agcgctaagt atccgttcgg tcacccgaac agcccgtaca ctggtgcgat taaacagggt 1020
gtttggaatc attttccggt tatggaaggt tgggatcaca tggacttcat taactttatc 1080
ggtagcaata ccccgggtta tttcagcatc tacggttttt acaacgacgt tgctaatcgt 1140
gttcatagcc tgtataaa                                               1158

 
<210> 1796
<211> 386
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of triacylglycerol lipase from Bacillus thermoamylovorans
 
<400> 1796
Met Glu Thr Ser Gly Asn Asp Tyr Pro Ile Val Leu Val His Gly Leu 
1               5                   10                  15      
Gly Gly Trp Gly Lys Gly Glu Met Leu Gly Tyr Arg Tyr Trp Gly Gly 
            20                  25                  30          
Leu Lys Asp Ile Glu Gly Tyr Leu Asn Gln Thr Gly His Arg Thr Tyr 
        35                  40                  45              
Thr Ala Thr Val Gly Pro Val Ser Ser Asn Trp Asp Arg Ala Val Glu 
    50                  55                  60                  
Leu Tyr Tyr Tyr Ile His Gly Gly Thr Val Asp Tyr Gly Ala Ala His 
65                  70                  75                  80  
Ala Lys Glu His Gly His Ala Arg Phe Gly Arg Thr Tyr Pro Gly Ile 
                85                  90                  95      
Tyr Gly Gln Trp Asp Glu Thr Asn Lys Ile His Leu Ile Gly His Ser 
            100                 105                 110         
Met Gly Gly Gln Thr Ser Arg Met Leu Val Glu Leu Leu Lys Ser Gly 
        115                 120                 125             
Ser Gln Lys Glu Gln Glu Tyr Tyr Ser Gln His Pro Glu Glu Gly Ile 
    130                 135                 140                 
Ser Pro Leu Phe Glu Gly Gly Lys Asn Trp Val His Ser Val Thr Ser 
145                 150                 155                 160 
Ile Ala Thr Pro His Asn Gly Ser Thr Phe Ala Asp Gln Arg Leu Ile 
                165                 170                 175     
Val Ser Phe Ile Lys Asp Phe Ile Ile His Leu Ala Ala Ala Ala Gly 
            180                 185                 190         
Leu Lys Gln Glu Ser Leu Ile Tyr Asp Phe Lys Leu Asp Gln Trp Gly 
        195                 200                 205             
Leu Lys Arg Gln Pro Gly Glu Ser Phe His Ala Tyr Met Asn Arg Val 
    210                 215                 220                 
Leu Thr Ser Pro Ile Trp Glu Ser Asn Asp Ile Ser Ala Tyr Asp Leu 
225                 230                 235                 240 
Thr Thr Arg Gly Ala Gln Glu Leu Asn Gln Trp Val Lys Thr Tyr Pro 
                245                 250                 255     
Asp Val Tyr Tyr Leu Ser Tyr Thr Gly Asn Ala Ser Tyr Arg Asp Val 
            260                 265                 270         
Val Thr Gly Asn Tyr Tyr Pro Ile Gly Thr Met His Pro Leu Leu Thr 
        275                 280                 285             
Leu Ile Ser Leu Arg Met Gly Arg Tyr Thr Arg Gln Ser Pro Pro Pro 
    290                 295                 300                 
Val Ile Asp Arg Ser Trp Leu Pro Asn Asp Gly Ile Val Asn Val Val 
305                 310                 315                 320 
Ser Ala Lys Tyr Pro Phe Gly His Pro Asn Ser Pro Tyr Thr Gly Ala 
                325                 330                 335     
Ile Lys Gln Gly Val Trp Asn His Phe Pro Val Met Glu Gly Trp Asp 
            340                 345                 350         
His Met Asp Phe Ile Asn Phe Ile Gly Ser Asn Thr Pro Gly Tyr Phe 
        355                 360                 365             
Ser Ile Tyr Gly Phe Tyr Asn Asp Val Ala Asn Arg Val His Ser Leu 
    370                 375                 380                 
Tyr Lys                                                         
385 