                         SEQUENCE LISTING

<110>  Alan Marc Kleinfeld
 
<120>  One step methods, kits, and systems for the measurement of 
concentrations of unbound bilirubin in biological fluids

<130>  FFASC.077WO

<140> PCT/US2020/048264   
<141> 2020-08-27

<150>  62/894,553
<151>  2019-08-30

<160>  29

<170>  PatentIn version 3.5

<210>  1
<211>  131
<212>  PRT
<213>  Rattus norvegicus

<220>
<221> PEPTIDE
<222> (1)...(131)
<223> wild-type rat intestinal fatty acid binding protein

<220>
<221> ACETYLATION
<222> (1)...(1)

<400>  1

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Leu Ala Asp Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Arg Ile Phe 
        115                 120                 125             

Lys Lys Glu 
    130                 

<210>  2
<211>  131
<212>  PRT
<213>  Artificial sequence

<220> 
<223> arginine substituting for fourteen accessible lysines

<400>  2

Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn Tyr Glu Arg
1               5                   10                  15      

Phe Met Glu Arg Met Gly Ile Asn Val Val Lys Arg Arg Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Arg Leu Thr Ile Thr Gln Glu Gly Asn Arg Phe Thr 
        35                  40                  45              

Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Leu Ala Asp Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe Arg Arg Val 
                85                  90                  95      

Asp Asn Gly Arg Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Arg Arg Ile Phe 
        115                 120                 125             

Arg Arg Glu 
    130  

<210>  3
<211>  28
<212>  PRT
<213>  Artificial sequence

<220> 
<223> C terminal double His tag linker

<400>  3

Arg Gly Ala Ala Ser His His His His His His Ser His Arg Ala Thr 
  1               5                  10                  15

Pro Asn Thr Ser Pro His His His His His His His
             20                  25    

<210>  4
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  4

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Cys Val Val Ala Arg Arg  
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His 
 

<210>  5
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  5

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Cys Val Ala Arg Arg  
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His 
    

<210>  6
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  6

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Cys Ala Arg Arg  
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His 


<210>  7
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  7

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Cys Arg Arg  
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His 


<210>  8
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  8

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Cys
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His 


<210>  9
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  9

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg
            20                  25                  30          

Cys Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His


<210>  10
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  10

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg
            20                  25                  30          

Leu Gly Ala Cys Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His


<210>  11
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  11

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Cys Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His


<210>  12
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  12

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Cys Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His


<210>  13
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  13

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Cys Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His


<210>  14
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  14

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Cys Asn Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His


<210>  15
<211>  162
<212>  PRT
<213>  Artificial sequence

<220> 
<223> mutated bf sensitive probe

<400>  15

Met Gly Ile Ala Phe Asp Gly Thr Trp Arg Val Asp Arg Asn Glu Asn 
1               5                   10                  15      

Arg Glu Arg Phe Leu Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg
            20                  25                  30          

Leu Gly Ala His Asp Asn Leu Arg Val Thr Ile Thr Gln Glu Gly Asn  
        35                  40                  45              

Arg Phe Thr Val Arg Glu Ser Ser Asn Phe Arg Asn Ile Asp Arg Val  
    50                  55                  60                  

Phe Glu Leu Gly Val Asp Phe Ala Tyr Ser Leu Phe Asp Gly Thr Glu  
65                  70                  75                  80  

Leu Thr Gly Thr Trp Thr Met Glu Gly Asn Arg Leu Val Gly Arg Phe  
                85                  90                  95      

Arg Arg Val Asp Cys Gly Arg Glu Leu Ile Ala Val Leu Glu Ile Ser  
            100                 105                 110         

Gly Asn Glu Leu Ile Arg Thr Asp Thr Tyr Glu Gly Val Glu Ala Arg  
        115                 120                 125             

Arg Ile Phe Arg Arg Asp Arg Gly Ala Ala Ser His His His His His  
    130                 135                 140                

His Ser His Arg Ala Thr Pro Asn Thr Ser Pro His His His His His 
145                 150                 155                 160

His His


<210>  16
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  16

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Cys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Met Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155    
   

<210>  17
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  17

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Cys Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Met Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155    
     

<210>  18
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  18

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

Cys Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Met Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  19
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  19

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Cys Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Met Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  20
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  20

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Met Cys Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  21
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  21

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Met Phe Gly Cys Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  22
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  22

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Met Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Cys Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  23
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  23

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Cys Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Val Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  24
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  24

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Val Cys Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  25
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  25

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Val Phe Gly Cys Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  26
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  26

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Val Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Cys Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  27
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  27

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Lys Met Gly Ile Asn Val Val Lys Arg Lys Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Cys Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Lys Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  28
<211>  159
<212>  PRT
<213>  Artificial sequence

<220> 
<223> nonresponder mutated probe

<400>  28

Ala Phe Asp Gly Thr Trp Lys Val Asp Arg Asn Glu Asn Tyr Glu Lys 
1               5                   10                  15      

Phe Met Glu Arg Met Gly Ile Asn Val Val Ala Arg Arg Leu Gly Ala 
            20                  25                  30          

His Asp Asn Leu Lys Leu Thr Ile Thr Gln Glu Gly Asn Lys Phe Thr 
        35                  40                  45              

Val Lys Glu Ser Ser Asn Phe Arg Asn Ile Asp Val Val Phe Glu Leu 
    50                  55                  60                  

Gly Val Asp Phe Ala Tyr Ser Ala Cys Phe Gly Thr Glu Leu Thr Gly 
65                  70                  75                  80  

Thr Trp Thr Met Glu Gly Asn Lys Leu Val Gly Lys Phe Lys Arg Val 
                85                  90                  95      

Asp Asn Gly Arg Glu Leu Ile Ala Val Arg Glu Ile Ser Gly Asn Glu 
            100                 105                 110         

Leu Ile Gln Thr Tyr Thr Tyr Glu Gly Val Glu Ala Lys Trp Ile Phe 
        115                 120                 125             

Lys Lys Asp Arg Gly Ala Ala Ser His His His His His His Ser His  
    130                 135                 140                  

Arg Ala Thr Pro Asn Thr Ser Pro His His His His His His His
145                 150                 155   


<210>  29
<211>  33
<212>  PRT
<213>  Artificial sequence

<220> 
<223> Hb blocking peptide

<400>  29

Asp Thr Glu Thr Asp Glu Tyr Ala Ala Ser His His His His His His 
  1               5                  10                  15

Ser His Arg Ala Thr Thr Pro Asn Thr Ser Pro His His His His His
             20                  25                  30

His
