               SEQUENCE LISTING

<110> Chr. Hansen A/S

<120> P6888 Novel cold-adapted Bacilli

<130> P6888

<160> 7

<170> BiSSAP 1.3.6

<210> 1
<211> 1032
<212> DNA
<213> Bacillus paralicheniformis


<220> 
<223> Heat-inducible transcription repressor HrcA nucleotide sequence
      in B. paralicheniformis CHCC3809

<400> 1
atgttaacaa atcgtcagct gttaatcttg caggttatcg tcaacgattt cattcgttca      60

gctcagccgg taggatcaag aacgctttcc aaaaaggaag atatcacatt cagctcagca     120

acgatcagaa acgaaatggc tgacttggaa gagctcggtt ttattgaaaa aacccactca     180

tcttcaggcc ggattccttc tgaaaaaggc tatcgctatt atgtcgatca tctgctttca     240

cccggaaagc tgtcaaaaac ggacttgaac attattcatt cggttttcaa agaaaaaatc     300

tttgaactcg aaaaagcggt gcagaagtcg gctcaagtgc tgtctgatct gacaaattat     360

acatcgattg tcctcggtcc gagactgagc gaaaatcatc tcaaacagat ccagattgtg     420

ccgattcagc ctaagaaggc cgttgccatt ctagtaacga ataccggcca tgtcgagaat     480

aaaacgatca actttccggc ggaggtcaat ctttccgatc tcgaaaagct ggtgaatata     540

ttaaatgaac gccttagagg cgtgccgatc tcagagctga aagacaggat tttcaaagag     600

gtcgtcatct tcttaaagtc gcatatccaa aattacgata cgattttaca cgggctcggc     660

gcaacgctgg attcatctgt tcaaaccgac cggctgtttt tcggcggcaa gattaatatg     720

ctgaatcagc ccgaatttca cgatattgac agagtgaaat cgctattgtc gctcattgag     780

aaagaacagg agcttctccg gctctttcag tcgactgagt ccggaattac cattaaaatc     840

ggcaaggaaa acgactatga agaaatggaa aactgcagcc tgattaccgc gacatacacg     900

gtcggttcaa aacagatcgg ctccatcgcg gtcatcgggc cgacgcgcat ggactactcc     960

cgcgtcgtcg gtttgcttca gcacgtatca tctgacttgt caaaagcgtt gacaagtttg    1020

tatgatgggt aa                                                        1032


<210> 2
<211> 343
<212> PRT
<213> Bacillus paralicheniformis


<220> 
<223> Heat-inducible transcription repressor HrcA protein sequence in
      B. paralicheniformis CHCC3809

<400> 2
Met Leu Thr Asn Arg Gln Leu Leu Ile Leu Gln Val Ile Val Asn Asp 
1               5                   10                  15      
Phe Ile Arg Ser Ala Gln Pro Val Gly Ser Arg Thr Leu Ser Lys Lys 
            20                  25                  30          
Glu Asp Ile Thr Phe Ser Ser Ala Thr Ile Arg Asn Glu Met Ala Asp 
        35                  40                  45              
Leu Glu Glu Leu Gly Phe Ile Glu Lys Thr His Ser Ser Ser Gly Arg 
    50                  55                  60                  
Ile Pro Ser Glu Lys Gly Tyr Arg Tyr Tyr Val Asp His Leu Leu Ser 
65                  70                  75                  80  
Pro Gly Lys Leu Ser Lys Thr Asp Leu Asn Ile Ile His Ser Val Phe 
                85                  90                  95      
Lys Glu Lys Ile Phe Glu Leu Glu Lys Ala Val Gln Lys Ser Ala Gln 
            100                 105                 110         
Val Leu Ser Asp Leu Thr Asn Tyr Thr Ser Ile Val Leu Gly Pro Arg 
        115                 120                 125             
Leu Ser Glu Asn His Leu Lys Gln Ile Gln Ile Val Pro Ile Gln Pro 
    130                 135                 140                 
Lys Lys Ala Val Ala Ile Leu Val Thr Asn Thr Gly His Val Glu Asn 
145                 150                 155                 160 
Lys Thr Ile Asn Phe Pro Ala Glu Val Asn Leu Ser Asp Leu Glu Lys 
                165                 170                 175     
Leu Val Asn Ile Leu Asn Glu Arg Leu Arg Gly Val Pro Ile Ser Glu 
            180                 185                 190         
Leu Lys Asp Arg Ile Phe Lys Glu Val Val Ile Phe Leu Lys Ser His 
        195                 200                 205             
Ile Gln Asn Tyr Asp Thr Ile Leu His Gly Leu Gly Ala Thr Leu Asp 
    210                 215                 220                 
Ser Ser Val Gln Thr Asp Arg Leu Phe Phe Gly Gly Lys Ile Asn Met 
225                 230                 235                 240 
Leu Asn Gln Pro Glu Phe His Asp Ile Asp Arg Val Lys Ser Leu Leu 
                245                 250                 255     
Ser Leu Ile Glu Lys Glu Gln Glu Leu Leu Arg Leu Phe Gln Ser Thr 
            260                 265                 270         
Glu Ser Gly Ile Thr Ile Lys Ile Gly Lys Glu Asn Asp Tyr Glu Glu 
        275                 280                 285             
Met Glu Asn Cys Ser Leu Ile Thr Ala Thr Tyr Thr Val Gly Ser Lys 
    290                 295                 300                 
Gln Ile Gly Ser Ile Ala Val Ile Gly Pro Thr Arg Met Asp Tyr Ser 
305                 310                 315                 320 
Arg Val Val Gly Leu Leu Gln His Val Ser Ser Asp Leu Ser Lys Ala 
                325                 330                 335     
Leu Thr Ser Leu Tyr Asp Gly 
            340             

<210> 3
<211> 1152
<212> DNA
<213> Bacillus paralicheniformis


<220> 
<223> BioF region 8-amino-7-oxononanoate synthase (EC_2.3.1.47)
      nucleotide sequence in B. paralicheniformis CHCC3809

<400> 3
gtgcccattg acgaatggct cagcagtcgg ctcgccagga cgaaggcagc cgggctttac      60

cgctcgctta aaccgcctca agctgtcgcg gaagcgaaac gtacgaatcg ggcgtcaaac     120

gattatttga gcctggcgaa cgacaaacgg ctgattcacg ccgcagaaac ggcgcttcgc     180

cgctttggag ccgggagcac agggtcaagg ctgacgtccg gcaataccga atggcatgaa     240

aaacttgagc ggaaaatcgc cggttttaag cagacagaag ccgctctctt gttttcaagc     300

ggatatttgg ccaatatcgg cgtgctttcg tctttgcctg aaaaaggaga cgtcatatta     360

agcgatcaat tgaaccatgc gagcatcatc gacggctgcc gcctttcaaa agctgatacg     420

gttgtttacc gccacacgga tatgaatgat cttgaagaaa aactccgcac cgcacagagc     480

cgtgctcgct gttttattgt gacagacggc gtgttcagca tggacggcac aattgcaccg     540

cttgacgaaa tcatgctcct tgccaagcgg taccgggcct ttgtcatcgt tgatgatgcc     600

cacgcaacag gggttttggg agatgccggc aggggaacgg gcgagtattt cggcgtctcc     660

ccagatgttg tgatcggcac gttaagtaaa gctgtaggcg cggaaggcgg cttcgtcgcg     720

ggctctaaag cattgatcga ttttttgctg aatcatgcga gaacgtttat ctttcaaacg     780

gctgtcccgc ccgcaagctg tgcggcggca tgcagagctt tagacatcat taaagacagc     840

cgggataaac gccggctttt gcaatcatcg gtgaccacga tcaaacgggg tctcgaggat     900

atcgggttta cggtcaaggg agaagatacg ccgatcatac ccgttatgat cggagaccct     960

caaaaagccg ttcgatttgc aaacggcctc aaggagaaag ggattgaggc cccggccatc    1020

cgcccgccga ctgttgcgga aggagagagc cgaatcaggc tgaccgtcac cgcagatcgc    1080

aagttaagag atattgaagc tctattagag gggtttaaat tagtgggaag agagttgaac    1140

ttggtgaaat ga                                                        1152


<210> 4
<211> 383
<212> PRT
<213> Bacillus paralicheniformis


<220> 
<223> BioF region 8-amino-7-oxononanoate synthase (EC_2.3.1.47) protein
      sequence in B. paralicheniformis CHCC3809

<400> 4
Met Pro Ile Asp Glu Trp Leu Ser Ser Arg Leu Ala Arg Thr Lys Ala 
1               5                   10                  15      
Ala Gly Leu Tyr Arg Ser Leu Lys Pro Pro Gln Ala Val Ala Glu Ala 
            20                  25                  30          
Lys Arg Thr Asn Arg Ala Ser Asn Asp Tyr Leu Ser Leu Ala Asn Asp 
        35                  40                  45              
Lys Arg Leu Ile His Ala Ala Glu Thr Ala Leu Arg Arg Phe Gly Ala 
    50                  55                  60                  
Gly Ser Thr Gly Ser Arg Leu Thr Ser Gly Asn Thr Glu Trp His Glu 
65                  70                  75                  80  
Lys Leu Glu Arg Lys Ile Ala Gly Phe Lys Gln Thr Glu Ala Ala Leu 
                85                  90                  95      
Leu Phe Ser Ser Gly Tyr Leu Ala Asn Ile Gly Val Leu Ser Ser Leu 
            100                 105                 110         
Pro Glu Lys Gly Asp Val Ile Leu Ser Asp Gln Leu Asn His Ala Ser 
        115                 120                 125             
Ile Ile Asp Gly Cys Arg Leu Ser Lys Ala Asp Thr Val Val Tyr Arg 
    130                 135                 140                 
His Thr Asp Met Asn Asp Leu Glu Glu Lys Leu Arg Thr Ala Gln Ser 
145                 150                 155                 160 
Arg Ala Arg Cys Phe Ile Val Thr Asp Gly Val Phe Ser Met Asp Gly 
                165                 170                 175     
Thr Ile Ala Pro Leu Asp Glu Ile Met Leu Leu Ala Lys Arg Tyr Arg 
            180                 185                 190         
Ala Phe Val Ile Val Asp Asp Ala His Ala Thr Gly Val Leu Gly Asp 
        195                 200                 205             
Ala Gly Arg Gly Thr Gly Glu Tyr Phe Gly Val Ser Pro Asp Val Val 
    210                 215                 220                 
Ile Gly Thr Leu Ser Lys Ala Val Gly Ala Glu Gly Gly Phe Val Ala 
225                 230                 235                 240 
Gly Ser Lys Ala Leu Ile Asp Phe Leu Leu Asn His Ala Arg Thr Phe 
                245                 250                 255     
Ile Phe Gln Thr Ala Val Pro Pro Ala Ser Cys Ala Ala Ala Cys Arg 
            260                 265                 270         
Ala Leu Asp Ile Ile Lys Asp Ser Arg Asp Lys Arg Arg Leu Leu Gln 
        275                 280                 285             
Ser Ser Val Thr Thr Ile Lys Arg Gly Leu Glu Asp Ile Gly Phe Thr 
    290                 295                 300                 
Val Lys Gly Glu Asp Thr Pro Ile Ile Pro Val Met Ile Gly Asp Pro 
305                 310                 315                 320 
Gln Lys Ala Val Arg Phe Ala Asn Gly Leu Lys Glu Lys Gly Ile Glu 
                325                 330                 335     
Ala Pro Ala Ile Arg Pro Pro Thr Val Ala Glu Gly Glu Ser Arg Ile 
            340                 345                 350         
Arg Leu Thr Val Thr Ala Asp Arg Lys Leu Arg Asp Ile Glu Ala Leu 
        355                 360                 365             
Leu Glu Gly Phe Lys Leu Val Gly Arg Glu Leu Asn Leu Val Lys 
    370                 375                 380             

<210> 5
<211> 383
<212> PRT
<213> Escherichia coli K12


<400> 5
Met Ser Trp Gln Glu Lys Ile Asn Ala Ala Leu Asp Ala Arg Arg Ala 
1               5                   10                  15      
Ala Asp Ala Leu Arg Arg Arg Tyr Pro Val Ala Gln Gly Ala Gly Arg 
            20                  25                  30          
Trp Leu Val Ala Asp Asp Arg Gln Tyr Leu Asn Phe Ser Ser Asn Asp 
        35                  40                  45              
Tyr Leu Gly Leu Ser His His Pro Gln Ile Ile Arg Ala Trp Gln Gln 
    50                  55                  60                  
Gly Ala Glu Gln Phe Gly Ile Gly Ser Gly Gly Ser Gly His Val Ser 
65                  70                  75                  80  
Gly Tyr Ser Val Val His Gln Ala Leu Glu Glu Glu Leu Ala Glu Trp 
                85                  90                  95      
Leu Gly Tyr Ser Arg Ala Leu Leu Phe Ile Ser Gly Phe Ala Ala Asn 
            100                 105                 110         
Gln Ala Trp Ile Ala Ala Met Met Ala Lys Glu Asp Arg Ile Ala Ala 
        115                 120                 125             
Asp Arg Leu Ser His Ala Ser Leu Leu Glu Ala Ala Ser Leu Ser Pro 
    130                 135                 140                 
Ser Gln Leu Arg Arg Phe Ala His Asn Asp Val Thr His Leu Ala Arg 
145                 150                 155                 160 
Leu Leu Ala Ser Pro Cys Pro Gly Gln Gln Met Val Val Thr Glu Gly 
                165                 170                 175     
Val Phe Ser Met Asp Gly Asp Ser Ala Pro Leu Ala Glu Ile Gln Gln 
            180                 185                 190         
Val Thr Gln Gln His Asn Gly Trp Leu Met Val Asp Asp Ala His Gly 
        195                 200                 205             
Thr Gly Val Ile Gly Glu Gln Gly Arg Gly Ser Cys Trp Leu Gln Lys 
    210                 215                 220                 
Val Lys Pro Glu Leu Leu Val Val Thr Phe Gly Lys Gly Phe Gly Val 
225                 230                 235                 240 
Ser Gly Ala Ala Val Leu Cys Ser Ser Thr Val Ala Asp Tyr Leu Leu 
                245                 250                 255     
Gln Phe Ala Arg His Leu Ile Tyr Ser Thr Met Pro Pro Ala Gln Ala 
            260                 265                 270         
Gln Ala Leu Arg Ala Ser Leu Ala Val Ile Arg Ser Asp Glu Gly Asp 
        275                 280                 285             
Ala Arg Arg Glu Lys Leu Ala Ala Leu Ile Thr Arg Phe Arg Ala Gly 
    290                 295                 300                 
Val Gln Asp Leu Pro Phe Thr Leu Ala Asp Ser Cys Ser Ala Ile Gln 
305                 310                 315                 320 
Pro Leu Ile Val Gly Asp Asn Ser Arg Ala Leu Gln Leu Ala Glu Lys 
                325                 330                 335     
Leu Arg Gln Gln Gly Cys Trp Val Thr Ala Ile Arg Pro Pro Thr Val 
            340                 345                 350         
Pro Ala Gly Thr Ala Arg Leu Arg Leu Thr Leu Thr Ala Ala His Glu 
        355                 360                 365             
Met Gln Asp Ile Asp Arg Leu Leu Glu Val Leu His Gly Asn Gly 
    370                 375                 380             

<210> 6
<211> 388
<212> PRT
<213> Bacillus subtilis


<400> 6
Met Lys Ile Asp Ser Trp Leu Asn Glu Arg Leu Asp Arg Met Lys Glu 
1               5                   10                  15      
Ala Gly Val His Arg Asn Leu Arg Ser Met Asp Gly Ala Pro Val Pro 
            20                  25                  30          
Glu Arg Asn Ile Asp Gly Glu Asn Gln Thr Val Trp Ser Ser Asn Asn 
        35                  40                  45              
Tyr Leu Gly Leu Ala Ser Asp Arg Arg Leu Ile Asp Ala Ala Gln Thr 
    50                  55                  60                  
Ala Leu Gln Gln Phe Gly Thr Gly Ser Ser Gly Ser Arg Leu Thr Thr 
65                  70                  75                  80  
Gly Asn Ser Trp Val His Glu Lys Leu Glu Lys Lys Ile Ala Ser Phe 
                85                  90                  95      
Lys Leu Thr Glu Ala Ala Leu Leu Phe Ser Ser Gly Tyr Leu Ala Asn 
            100                 105                 110         
Val Gly Val Leu Ser Ser Leu Pro Glu Lys Glu Asp Val Ile Leu Ser 
        115                 120                 125             
Asp Gln Leu Asn His Ala Ser Met Ile Asp Gly Cys Arg Leu Ser Lys 
    130                 135                 140                 
Ala Asp Thr Val Val Tyr Arg His Ile Asp Met Asn Asp Leu Glu Asn 
145                 150                 155                 160 
Lys Leu Asn Glu Thr Gln Arg Tyr Lys Arg Arg Phe Ile Val Thr Asp 
                165                 170                 175     
Gly Val Phe Ser Met Asp Gly Thr Ile Ala Pro Leu Asp Gln Ile Ile 
            180                 185                 190         
Ser Leu Ala Lys Arg Tyr His Ala Phe Val Val Val Asp Asp Ala His 
        195                 200                 205             
Ala Thr Gly Val Leu Gly Asp Ser Gly Gln Gly Thr Ser Glu Tyr Phe 
    210                 215                 220                 
Gly Val Cys Pro Asp Ile Val Ile Gly Thr Leu Ser Lys Ala Val Gly 
225                 230                 235                 240 
Ala Glu Gly Gly Phe Ala Ala Gly Ser Ala Val Phe Ile Asp Phe Leu 
                245                 250                 255     
Leu Asn His Arg Thr Phe Ile Phe Gln Thr Ala Ile Pro Pro Ala Ser 
            260                 265                 270         
Cys Ala Ala Ala His Glu Ala Phe Asn Ile Ile Glu Ala Ser Arg Glu 
        275                 280                 285             
Lys Arg Gln Leu Leu Phe Ser Tyr Ile Ser Met Ile Arg Thr Ser Leu 
    290                 295                 300                 
Lys Asn Met Gly Tyr Val Val Lys Gly Asp His Thr Pro Ile Ile Pro 
305                 310                 315                 320 
Val Val Ile Gly Asp Ala His Lys Thr Val Leu Phe Ala Glu Lys Leu 
                325                 330                 335     
Gln Gly Lys Gly Ile Tyr Ala Pro Ala Ile Arg Pro Pro Thr Val Ala 
            340                 345                 350         
Pro Gly Glu Ser Arg Ile Arg Ile Thr Ile Thr Ser Asp His Ser Met 
        355                 360                 365             
Gly Asp Ile Asp His Leu Leu Gln Thr Phe His Ser Ile Gly Lys Glu 
    370                 375                 380                 
Leu His Ile Ile 
385             

<210> 7
<211> 383
<212> PRT
<213> Bacillus paralicheniformis 


<220> 
<223> DSM31110

<400> 7
Met Pro Ile Asp Glu Trp Leu Ser Ser Arg Leu Ala Arg Thr Lys Ala 
1               5                   10                  15      
Ala Gly Leu Tyr Arg Ser Leu Lys Pro Pro Gln Ala Val Ala Glu Ala 
            20                  25                  30          
Lys Arg Thr Asn Arg Ala Ser Asn Asp Tyr Leu Ser Leu Ala Asn Asp 
        35                  40                  45              
Lys Arg Leu Ile His Ala Ala Glu Thr Ala Leu Arg Arg Phe Gly Ala 
    50                  55                  60                  
Gly Ser Thr Gly Ser Arg Leu Thr Ser Gly Asn Thr Glu Trp His Glu 
65                  70                  75                  80  
Lys Leu Glu Arg Lys Ile Ala Gly Phe Lys Gln Thr Glu Ala Ala Leu 
                85                  90                  95      
Leu Phe Ser Ser Gly Tyr Leu Ala Asn Ile Gly Val Leu Ser Ser Leu 
            100                 105                 110         
Pro Glu Lys Gly Asp Val Ile Leu Ser Asp Gln Leu Asn His Ala Ser 
        115                 120                 125             
Ile Ile Asp Gly Cys Arg Leu Ser Lys Ala Asp Thr Val Val Tyr Arg 
    130                 135                 140                 
His Thr Asp Met Asn Asp Leu Glu Glu Lys Leu Arg Thr Ala Gln Ser 
145                 150                 155                 160 
Arg Ala Arg Cys Phe Ile Val Thr Asp Gly Val Phe Ser Met Asp Gly 
                165                 170                 175     
Thr Ile Ala Pro Leu Asp Glu Ile Met Leu Leu Ala Lys Arg Tyr Arg 
            180                 185                 190         
Ala Phe Val Ile Val Asp Asp Ala His Ala Thr Gly Val Leu Gly Asp 
        195                 200                 205             
Ala Gly Arg Gly Thr Gly Glu Tyr Phe Gly Val Ser Pro Asp Val Val 
    210                 215                 220                 
Ile Gly Thr Leu Ser Lys Ala Val Gly Ala Glu Gly Gly Phe Val Ala 
225                 230                 235                 240 
Gly Ser Lys Ala Leu Ile Asp Phe Leu Leu Asn His Ala Arg Thr Phe 
                245                 250                 255     
Ile Phe Gln Thr Ala Val Pro Pro Ala Ser Cys Ala Ala Ala Cys Arg 
            260                 265                 270         
Ala Leu Asp Ile Ile Lys Asp Ser Arg Asp Lys Arg Arg Leu Leu Gln 
        275                 280                 285             
Ser Ser Val Thr Thr Ile Lys Arg Gly Leu Glu Asp Ile Gly Phe Thr 
    290                 295                 300                 
Val Lys Gly Glu Asp Thr Pro Ile Ile Pro Val Met Ile Gly Asp Pro 
305                 310                 315                 320 
Gln Lys Ala Val Arg Phe Ala Asn Gly Leu Lys Glu Lys Gly Ile Glu 
                325                 330                 335     
Ala Pro Ala Ile Arg Pro Pro Thr Val Ala Glu Gly Glu Ser Arg Ile 
            340                 345                 350         
Arg Leu Thr Val Thr Ala Asp Arg Lys Leu Arg Asp Ile Glu Ala Leu 
        355                 360                 365             
Leu Glu Gly Phe Lys Leu Val Gly Arg Glu Leu Asn Leu Val Lys 
    370                 375                 380             

