                         SEQUENCE LISTING

<110>  Carlsberg A/S
 
<120>  Plant with low nitrogen levels

<130>  P4541PC00

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  406
<212>  PRT
<213>  Hordeum vulgare cv. Optic

<400>  1

Met Gly Ser Pro Asp Ser Ser Ser Gly Ser Ala Gln Lys Pro Pro Arg 
1               5                   10                  15      


His Gln His Gln His Gln Pro Pro Pro Pro Arg Arg Gln Gly Ser Ala 
            20                  25                  30          


Pro Glu Leu Pro Pro Gly Phe Arg Phe His Pro Thr Asp Glu Glu Leu 
        35                  40                  45              


Val Val His Tyr Leu Lys Lys Lys Ala Ala Lys Ala Pro Leu Pro Val 
    50                  55                  60                  


Thr Ile Ile Ala Glu Val Asp Leu Tyr Lys Phe Asp Pro Trp Glu Leu 
65                  70                  75                  80  


Pro Glu Lys Ala Thr Phe Gly Glu His Glu Trp Tyr Phe Phe Ser Pro 
                85                  90                  95      


Arg Asp Arg Lys Tyr Ala Asn Gly Ala Arg Pro Asn Arg Ala Ala Thr 
            100                 105                 110         


Ser Gly Tyr Trp Lys Ala Thr Gly Thr Asp Lys Pro Ile Leu Ala Ser 
        115                 120                 125             


Ala Thr Gly Cys Gly Arg Glu Lys Val Gly Val Lys Lys Ala Leu Val 
    130                 135                 140                 


Phe Tyr Arg Gly Lys Pro Pro Arg Gly Leu Lys Thr Asn Trp Ile Met 
145                 150                 155                 160 


His Glu Tyr Arg Leu Thr Gly Ala Ser Ala Gly Ser Thr Thr Thr Ser 
                165                 170                 175     


Arg Pro Pro Pro Val Thr Gly Gly Ser Arg Ala Pro Ala Ser Leu Arg 
            180                 185                 190         


Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr Lys Lys Thr Ser Lys Ala 
        195                 200                 205             


Ala Ala Ala Val Gly Asp Glu Gln Arg Ser Met Glu Cys Glu Asp Ser 
    210                 215                 220                 


Val Glu Asp Ala Val Thr Ala Tyr Pro Pro Tyr Ala Thr Ala Gly Met 
225                 230                 235                 240 


Ala Gly Ala Gly Ala His Gly Ser Asn Tyr Val Gln Leu Leu His His 
                245                 250                 255     


His Asp Ser His Glu Asp Asn Phe Gln Leu Asp Gly Leu Leu Thr Glu 
            260                 265                 270         


His Asp Val Gly Leu Ser Ala Gly Ala Ala Ser Leu Gly His Leu Ala 
        275                 280                 285             


Ala Ala Ala Arg Ala Thr Lys Gln Phe Leu Ala Pro Ser Ser Ser Thr 
    290                 295                 300                 


Pro Phe Asn Trp Leu Glu Ala Ser Thr Gly Gly Ser Ile Leu Pro Gln 
305                 310                 315                 320 


Ala Arg Asn Phe Pro Gly Phe Asn Arg Ser Arg Asn Val Gly Ser Met 
                325                 330                 335     


Ser Leu Ser Ser Thr Ala Asp Asp Met Ala Gly Ala Val Asp Val Ser 
            340                 345                 350         


Asp Gly Gly Asn Ala Val Asn Ala Met Tyr Leu Pro Val Gln Asp Gly 
        355                 360                 365             


Thr Tyr His Gln His Val Ile Leu Gly Ala Pro Leu Ala Pro Glu Ala 
    370                 375                 380                 


Ile Ala Gly Ala Ala Thr Ser Gly Phe Gln His His Val Gln Ile Ser 
385                 390                 395                 400 


Gly Val Asn Trp Asn Pro 
                405     


<210>  2
<211>  1221
<212>  DNA
<213>  Hordeum vulgare cv. Optic

<400>  2
atgggcagcc cggactcatc ctccggctcg gcgcaaaaac caccgcggca tcagcatcag       60

catcagccgc cgcctccgcg gcggcagggc tcggcgccgg agctccctcc cggcttccgg      120

ttccacccga cggacgagga gctggtcgtg cactacctca agaagaaggc cgccaaggcg      180

ccgctccccg tcaccatcat cgccgaggtg gacctctaca agttcgaccc atgggagctc      240

cccgagaagg cgaccttcgg ggagcacgag tggtacttct tcagcccgcg cgaccgcaag      300

tacgccaacg gcgcgcggcc gaaccgggcg gcgacgtcgg gctactggaa ggccaccggc      360

acggacaagc ctatcctggc ctcggccacc gggtgcggcc gggagaaggt cggcgtcaag      420

aaggcgctcg tcttctaccg cgggaagccg cccaggggcc tcaagaccaa ctggatcatg      480

catgagtacc gcctcaccgg agcctctgct ggctccacca ccaccagccg gccgccgccg      540

gtgaccggcg ggagcagggc cccggcctct ctcaggttgg acgactgggt gctgtgccgc      600

atctacaaga agaccagcaa ggccgcggcc gcggtcggag atgagcagag gagcatggag      660

tgcgaggact ccgtggagga cgcggtcacc gcgtacccgc cctacgccac ggcgggcatg      720

gccggcgcag gtgcgcatgg cagcaactac gttcaactgc tccatcatca cgacagccac      780

gaggacaact tccagctaga cggcctgctc acagaacacg acgtcggcct ctcggcgggc      840

gccgcctcgc tgggccacct tgccgcggcg gcgagggcca ccaaacagtt cctcgccccg      900

tcgtcctcaa ccccgttcaa ctggctcgag gcgtcaaccg gtggcagcat cctcccacag      960

gcaaggaatt tccctgggtt taacaggagc agaaacgtcg gcagtatgtc gctgtcatcc     1020

acggccgacg acatggctgg cgcggtggac gtcagcgacg gaggcaatgc ggtgaacgcc     1080

atgtatctcc ccgtgcaaga cgggacctac catcagcatg tcatcctcgg agctccgctg     1140

gcgccagagg ccatcgcggg cgccgccacc tctggtttcc agcatcacgt ccaaatatcc     1200

ggcgtgaact ggaatccctg a                                               1221


<210>  3
<211>  1585
<212>  DNA
<213>  Hordeum vulgare cv. Optic

<400>  3
atgggcagcc cggactcatc ctccggctcg gcgcaaaaac caccgcggca tcagcatcag       60

catcagccgc cgcctccgcg gcggcagggc tcggcgccgg agctccctcc cggcttccgg      120

ttccacccga cggacgagga gctggtcgtg cactacctca agaagaaggc cgccaaggcg      180

ccgctccccg tcaccatcat cgccgaggtg gacctctaca agttcgaccc atgggagctc      240

cccggtatgt actactagtt agtactatgt ctatccctat ctcgtcgatc gtgcttgctt      300

gctctatcaa gcgccgtaat ttcccggtgc aattaaataa tcgaatccgt ccacgcatcc      360

atccatcatg ctttttatta tactgtgcac aagtattttt atattcttcc agtaagtaca      420

gcgcatgtat gtgatcctgt cgtcgtgctt gttcatgcgc tcgggcggga tcatcatcca      480

tcagagaagg cgaccttcgg ggagcacgag tggtacttct tcagcccgcg cgaccgcaag      540

tacgccaacg gcgcgcggcc gaaccgggcg gcgacgtcgg gctactggaa ggccaccggc      600

acggacaagc ctatcctggc ctcggccacc gggtgcggcc gggagaaggt cggcgtcaag      660

aaggcgctcg tcttctaccg cgggaagccg cccaggggcc tcaagaccaa ctggatcatg      720

catgagtacc gcctcaccgg agcctctgct ggctccacca ccaccagccg gccgccgccg      780

gtgaccggcg ggagcagggc cccggcctct ctcagggtac gtacttacac gtgtccatcg      840

cacggtctat cagtatttat ttattaacta ctctcgagct taattatggt attgttgata      900

gttgatgaag ttaattattg tacgccgtct catcgatcag ttggacgact gggtgctgtg      960

ccgcatctac aagaagacca gcaaggccgc ggccgcggtc ggagatgagc agaggagcat     1020

ggagtgcgag gactccgtgg aggacgcggt caccgcgtac ccgccctacg ccacggcggg     1080

catggccggc gcaggtgcgc atggcagcaa ctacgttcaa ctgctccatc atcacgacag     1140

ccacgaggac aacttccagc tagacggcct gctcacagaa cacgacgtcg gcctctcggc     1200

gggcgccgcc tcgctgggcc accttgccgc ggcggcgagg gccaccaaac agttcctcgc     1260

cccgtcgtcc tcaaccccgt tcaactggct cgaggcgtca accggtggca gcatcctccc     1320

acaggcaagg aatttccctg ggtttaacag gagcagaaac gtcggcagta tgtcgctgtc     1380

atccacggcc gacgacatgg ctggcgcggt ggacgtcagc gacggaggca atgcggtgaa     1440

cgccatgtat ctccccgtgc aagacgggac ctaccatcag catgtcatcc tcggagctcc     1500

gctggcgcca gaggccatcg cgggcgccgc cacctctggt ttccagcatc acgtccaaat     1560

atccggcgtg aactggaatc cctga                                           1585


<210>  4
<211>  406
<212>  PRT
<213>  Hordeum vulgare, haplotype 2

<400>  4

Met Gly Ser Pro Asp Ser Ser Ser Gly Ser Ala Gln Lys Pro Pro Arg 
1               5                   10                  15      


His Gln His Gln His Gln Pro Pro Pro Pro Arg Arg Gln Gly Ser Ala 
            20                  25                  30          


Pro Glu Leu Pro Pro Gly Phe Arg Phe His Pro Thr Asp Glu Glu Leu 
        35                  40                  45              


Val Val His Tyr Leu Lys Lys Lys Ala Ala Lys Ala Pro Leu Pro Val 
    50                  55                  60                  


Thr Ile Ile Ala Glu Val Asp Leu Tyr Lys Phe Asp Pro Trp Glu Leu 
65                  70                  75                  80  


Pro Glu Lys Ala Thr Phe Gly Glu His Glu Trp Tyr Phe Phe Ser Pro 
                85                  90                  95      


Arg Asp Arg Lys Tyr Pro Asn Gly Ala Arg Pro Asn Arg Ala Ala Thr 
            100                 105                 110         


Ser Gly Tyr Trp Lys Ala Thr Gly Thr Asp Lys Pro Ile Leu Ala Ser 
        115                 120                 125             


Ala Thr Gly Cys Gly Arg Glu Lys Val Gly Val Lys Lys Ala Leu Val 
    130                 135                 140                 


Phe Tyr Arg Gly Lys Pro Pro Arg Gly Leu Lys Thr Asn Trp Ile Met 
145                 150                 155                 160 


His Glu Tyr Arg Leu Thr Gly Ala Ser Ala Gly Ser Thr Thr Thr Ser 
                165                 170                 175     


Arg Pro Pro Pro Val Thr Gly Gly Ser Arg Ala Pro Ala Ser Leu Arg 
            180                 185                 190         


Leu Asp Asp Trp Val Leu Cys Arg Ile Tyr Lys Lys Thr Ser Lys Ala 
        195                 200                 205             


Ala Ala Ala Val Gly Asp Glu Gln Arg Ser Met Glu Cys Glu Asp Ser 
    210                 215                 220                 


Val Glu Asp Ala Val Thr Ala Tyr Pro Pro Tyr Ala Thr Ala Gly Met 
225                 230                 235                 240 


Ala Gly Ala Gly Ala His Gly Ser Asn Tyr Val Gln Leu Leu His His 
                245                 250                 255     


His Asp Ser His Glu Asp Asn Phe Gln Leu Asp Gly Leu Leu Thr Glu 
            260                 265                 270         


His Asp Val Gly Leu Ser Ala Gly Ala Ala Ser Leu Gly His Leu Ala 
        275                 280                 285             


Ala Ala Ala Arg Ala Thr Lys Gln Phe Leu Ala Pro Ser Ser Ser Thr 
    290                 295                 300                 


Pro Phe Asn Trp Leu Glu Ala Ser Thr Gly Gly Ser Ile Leu Pro Gln 
305                 310                 315                 320 


Ala Arg Asn Phe Pro Gly Phe Asn Arg Ser Arg Asn Val Gly Ser Met 
                325                 330                 335     


Ser Leu Ser Ser Thr Ala Asp Asp Met Ala Gly Ala Val Asp Val Ser 
            340                 345                 350         


Asp Gly Gly Asn Ala Val Asn Ala Met Tyr Leu Pro Val Gln Asp Gly 
        355                 360                 365             


Thr Tyr His Gln His Val Ile Leu Gly Ala Pro Leu Ala Pro Glu Ala 
    370                 375                 380                 


Ile Ala Gly Ala Ala Thr Ser Gly Phe Gln His His Val Gln Ile Ser 
385                 390                 395                 400 


Gly Val Asn Trp Asn Pro 
                405     


<210>  5
<211>  13
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 3 forward primer


<220>
<221>  misc_feature
<222>  (1)..(13)
<223>  Mutant 3 forward primer

<400>  5
ccgctccccg tca                                                          13


<210>  6
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 3 reverse primer


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  Mutant 3 revers primer

<400>  6
aactagtagt acataccggg                                                   20


<210>  7
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 3 FAM probe


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  Mutant 3 FAM probe

<400>  7
cgccgagatg gacct                                                        15


<210>  8
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 3 HEX probe


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  Mutant 3 HEX probe

<400>  8
tcgccgaggt ggac                                                         14


<210>  9
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 4 forward primer


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  Mutant 4 forward primer

<400>  9
cttccggttc caccc                                                        15


<210>  10
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 4 reverse primer


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  Mutant 4 reverse primer

<400>  10
ggcggccttc ttctt                                                        15


<210>  11
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 4 FAM probe


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  Mutant 4 FAM probe

<400>  11
cgaggagctg atcgtg                                                       16


<210>  12
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 4 HEX probe


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  Mutant 4 HEX probe

<400>  12
cgaggagctg gtcgt                                                        15


<210>  13
<211>  16
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 5 forward primer


<220>
<221>  misc_feature
<222>  (1)..(16)
<223>  Mutant 5 forward primer

<400>  13
gtcgggctac tggaag                                                       16


<210>  14
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 5 reverse primer


<220>
<221>  misc_feature
<222>  (1)..(12)
<223>  Mutant 5 reverse primer

<400>  14
acccggtggc cg                                                           12


<210>  15
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 5 FAM probe


<220>
<221>  misc_feature
<222>  (1)..(15)
<223>  Mutant 5 FAM probe

<400>  15
accggcacga acaag                                                        15


<210>  16
<211>  14
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Mutant 5 HEX probe


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  Mutant 5 HEX probe

<400>  16
accggcacgg acaa                                                         14


<210>  17
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  forward primer HvNAM-1


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  forward primer HvNAM-1

<400>  17
gccgtctcat cgatcagttg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  reverse primer HvNAM-1


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  reverse primer HvNAM-1

<400>  18
tggtgtcatt cgttcaggga                                                   20


