﻿                         序列表

<110>  黑龙江伊品生物科技有限公司

<120>  基于大肠杆菌的重组菌株及其构建方法与应用

<130>  CPWO20110939

<160>  32

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1278
<212>  DNA
<213>  Escherichia coli

<400>  1
atgctcgaat tgctttacac cgcccttctc taccttattc agccgctgat ctggatacgg        60
ctctgggtgc gcggacgtaa ggctccggcc tatcgaaaac gctggggtga acgttacggt       120
ttttaccgcc atccgctaaa accaggcggc attatgctgc actccgtctc cgtcggtgaa       180
actctggcgg caatcccgtt ggtgcgcgcg ctgcgtcatc gttatcctga tttaccgatt       240
accgtaacaa ccatgacgcc aaccggttcg gagcgcgtac aatcggcttt cgggaaggat       300
gttcagcacg tttatctgcc gtatgatctg cccgatgcac tcaaccgttt cctgaataaa       360
gtcgacccta aactggtgtt gattatggaa accgaactat ggcctaacct gattgcggcg       420
ctacataaac gtaaaattcc gctggtgatc gctaacgcgc gactctctgc ccgctcggcc       480
gcaggttatg ccaaactggg taaattcgtc cgtcgcttgc tgcgtcgtat tacgctgatt       540
gctgcgcaaa atgaagaaga tggtgcacgt tttgtggcgc tgggcgcaaa aaataatcag       600
gtgaccgtta ccggtagcct gaaattcgat atttctgtaa cgccgcagtt ggctgctaaa       660
gccgtgacgc tgcgccgcca gtgggcacca caccgcccgg tatggattgc caccagcact       720
cacgaaggcg aagagagtgt ggtgatcgcc gcacatcagg cattgttaca gcaattcccg       780
aatttattgc tcatcctggt accccgtcat ccggaacgct tcccggatgc gattaacctt       840
gtccgccagg ctggactaag ctatatcaca cgctcttcag gggaagtccc ctccaccagc       900
acgcaggttg tggttggcga tacgatgggc gagttgatgt tactgtatgg cattgccgat       960
ctcgcctttg ttggcggttc actggttgaa cgtggtgggc ataatccgct ggaagctgcc      1020
gcacacgcta ttccggtatt gatggggccg catactttta actttaaaga catttgcgcg      1080
cggctggagc aggcaagcgg gctgattacc gttaccgatg ccactacgct tgcaaaagag      1140
gtttcctctt tactcaccga cgccgattac cgtagtttct atggccgtca tgccgttgaa      1200
gtactgtatc aaaaccaggg cgcgctacag cgtctgcttc aactgctgga accttacctg      1260
ccaccgaaaa cgcattga                                                    1278

<210>  2
<211>  1278
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
atgctcgaat tgctttacac cgcccttctc taccttattc agccgctgat ctggatacgg        60
ctctgggtgc gcggacgtaa gactccggcc tatcgaaaac gctggggtga acgttacggt       120
ttttaccgcc atccgctaaa accaggcggc attatgctgc actccgtctc cgtcggtgaa       180
actctggcgg caatcccgtt ggtgcgcgcg ctgcgtcatc gttatcctga tttaccgatt       240
accgtaacaa ccatgacgcc aaccggttcg gagcgcgtac aatcggcttt cgggaaggat       300
gttcagcacg tttatctgcc gtatgatctg cccgatgcac tcaaccgttt cctgaataaa       360
gtcgacccta aactggtgtt gattatggaa accgaactat ggcctaacct gattgcggcg       420
ctacataaac gtaaaattcc gctggtgatc gctaacgcgc gactctctgc ccgctcggcc       480
gcaggttatg ccaaactggg taaattcgtc cgtcgcttgc tgcgtcgtat tacgctgatt       540
gctgcgcaaa atgaagaaga tggtgcacgt tttgtggcgc tgggcgcaaa aaataatcag       600
gtgaccgtta ccggtagcct gaaattcgat atttctgtaa cgccgcagtt ggctgctaaa       660
gccgtgacgc tgcgccgcca gtgggcacca caccgcccgg tatggattgc caccagcact       720
cacgaaggcg aagagagtgt ggtgatcgcc gcacatcagg cattgttaca gcaattcccg       780
aatttattgc tcatcctggt accccgtcat ccggaacgct tcccggatgc gattaacctt       840
gtccgccagg ctggactaag ctatatcaca cgctcttcag gggaagtccc ctccaccagc       900
acgcaggttg tggttggcga tacgatgggc gagttgatgt tactgtatgg cattgccgat       960
ctcgcctttg ttggcggttc actggttgaa cgtggtgggc ataatccgct ggaagctgcc      1020
gcacacgcta ttccggtatt gatggggccg catactttta actttaaaga catttgcgcg      1080
cggctggagc aggcaagcgg gctgattacc gttaccgatg ccactacgct tgcaaaagag      1140
gtttcctctt tactcaccga cgccgattac cgtagtttct atggccgtca tgccgttgaa      1200
gtactgtatc aaaaccaggg cgcgctacag cgtctgcttc aactgctgga accttacctg      1260
ccaccgaaaa cgcattga                                                    1278

<210>  3
<211>  425
<212>  PRT
<213>  Escherichia coli

<400>  3
Met Leu Glu Leu Leu Tyr Thr Ala Leu Leu Tyr Leu Ile Gln Pro Leu 
1               5                   10                  15      


Ile Trp Ile Arg Leu Trp Val Arg Gly Arg Lys Ala Pro Ala Tyr Arg 
            20                  25                  30          


Lys Arg Trp Gly Glu Arg Tyr Gly Phe Tyr Arg His Pro Leu Lys Pro 
        35                  40                  45              


Gly Gly Ile Met Leu His Ser Val Ser Val Gly Glu Thr Leu Ala Ala 
    50                  55                  60                  


Ile Pro Leu Val Arg Ala Leu Arg His Arg Tyr Pro Asp Leu Pro Ile 
65                  70                  75                  80  


Thr Val Thr Thr Met Thr Pro Thr Gly Ser Glu Arg Val Gln Ser Ala 
                85                  90                  95      


Phe Gly Lys Asp Val Gln His Val Tyr Leu Pro Tyr Asp Leu Pro Asp 
            100                 105                 110         


Ala Leu Asn Arg Phe Leu Asn Lys Val Asp Pro Lys Leu Val Leu Ile 
        115                 120                 125             


Met Glu Thr Glu Leu Trp Pro Asn Leu Ile Ala Ala Leu His Lys Arg 
    130                 135                 140                 


Lys Ile Pro Leu Val Ile Ala Asn Ala Arg Leu Ser Ala Arg Ser Ala 
145                 150                 155                 160 


Ala Gly Tyr Ala Lys Leu Gly Lys Phe Val Arg Arg Leu Leu Arg Arg 
                165                 170                 175     


Ile Thr Leu Ile Ala Ala Gln Asn Glu Glu Asp Gly Ala Arg Phe Val 
            180                 185                 190         


Ala Leu Gly Ala Lys Asn Asn Gln Val Thr Val Thr Gly Ser Leu Lys 
        195                 200                 205             


Phe Asp Ile Ser Val Thr Pro Gln Leu Ala Ala Lys Ala Val Thr Leu 
    210                 215                 220                 


Arg Arg Gln Trp Ala Pro His Arg Pro Val Trp Ile Ala Thr Ser Thr 
225                 230                 235                 240 


His Glu Gly Glu Glu Ser Val Val Ile Ala Ala His Gln Ala Leu Leu 
                245                 250                 255     


Gln Gln Phe Pro Asn Leu Leu Leu Ile Leu Val Pro Arg His Pro Glu 
            260                 265                 270         


Arg Phe Pro Asp Ala Ile Asn Leu Val Arg Gln Ala Gly Leu Ser Tyr 
        275                 280                 285             


Ile Thr Arg Ser Ser Gly Glu Val Pro Ser Thr Ser Thr Gln Val Val 
    290                 295                 300                 


Val Gly Asp Thr Met Gly Glu Leu Met Leu Leu Tyr Gly Ile Ala Asp 
305                 310                 315                 320 


Leu Ala Phe Val Gly Gly Ser Leu Val Glu Arg Gly Gly His Asn Pro 
                325                 330                 335     


Leu Glu Ala Ala Ala His Ala Ile Pro Val Leu Met Gly Pro His Thr 
            340                 345                 350         


Phe Asn Phe Lys Asp Ile Cys Ala Arg Leu Glu Gln Ala Ser Gly Leu 
        355                 360                 365             


Ile Thr Val Thr Asp Ala Thr Thr Leu Ala Lys Glu Val Ser Ser Leu 
    370                 375                 380                 


Leu Thr Asp Ala Asp Tyr Arg Ser Phe Tyr Gly Arg His Ala Val Glu 
385                 390                 395                 400 


Val Leu Tyr Gln Asn Gln Gly Ala Leu Gln Arg Leu Leu Gln Leu Leu 
                405                 410                 415     


Glu Pro Tyr Leu Pro Pro Lys Thr His 
            420                 425 

<210>  4
<211>  425
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  4
Met Leu Glu Leu Leu Tyr Thr Ala Leu Leu Tyr Leu Ile Gln Pro Leu 
1               5                   10                  15      


Ile Trp Ile Arg Leu Trp Val Arg Gly Arg Lys Thr Pro Ala Tyr Arg 
            20                  25                  30          


Lys Arg Trp Gly Glu Arg Tyr Gly Phe Tyr Arg His Pro Leu Lys Pro 
        35                  40                  45              


Gly Gly Ile Met Leu His Ser Val Ser Val Gly Glu Thr Leu Ala Ala 
    50                  55                  60                  


Ile Pro Leu Val Arg Ala Leu Arg His Arg Tyr Pro Asp Leu Pro Ile 
65                  70                  75                  80  


Thr Val Thr Thr Met Thr Pro Thr Gly Ser Glu Arg Val Gln Ser Ala 
                85                  90                  95      


Phe Gly Lys Asp Val Gln His Val Tyr Leu Pro Tyr Asp Leu Pro Asp 
            100                 105                 110         


Ala Leu Asn Arg Phe Leu Asn Lys Val Asp Pro Lys Leu Val Leu Ile 
        115                 120                 125             


Met Glu Thr Glu Leu Trp Pro Asn Leu Ile Ala Ala Leu His Lys Arg 
    130                 135                 140                 


Lys Ile Pro Leu Val Ile Ala Asn Ala Arg Leu Ser Ala Arg Ser Ala 
145                 150                 155                 160 


Ala Gly Tyr Ala Lys Leu Gly Lys Phe Val Arg Arg Leu Leu Arg Arg 
                165                 170                 175     


Ile Thr Leu Ile Ala Ala Gln Asn Glu Glu Asp Gly Ala Arg Phe Val 
            180                 185                 190         


Ala Leu Gly Ala Lys Asn Asn Gln Val Thr Val Thr Gly Ser Leu Lys 
        195                 200                 205             


Phe Asp Ile Ser Val Thr Pro Gln Leu Ala Ala Lys Ala Val Thr Leu 
    210                 215                 220                 


Arg Arg Gln Trp Ala Pro His Arg Pro Val Trp Ile Ala Thr Ser Thr 
225                 230                 235                 240 


His Glu Gly Glu Glu Ser Val Val Ile Ala Ala His Gln Ala Leu Leu 
                245                 250                 255     


Gln Gln Phe Pro Asn Leu Leu Leu Ile Leu Val Pro Arg His Pro Glu 
            260                 265                 270         


Arg Phe Pro Asp Ala Ile Asn Leu Val Arg Gln Ala Gly Leu Ser Tyr 
        275                 280                 285             


Ile Thr Arg Ser Ser Gly Glu Val Pro Ser Thr Ser Thr Gln Val Val 
    290                 295                 300                 


Val Gly Asp Thr Met Gly Glu Leu Met Leu Leu Tyr Gly Ile Ala Asp 
305                 310                 315                 320 


Leu Ala Phe Val Gly Gly Ser Leu Val Glu Arg Gly Gly His Asn Pro 
                325                 330                 335     


Leu Glu Ala Ala Ala His Ala Ile Pro Val Leu Met Gly Pro His Thr 
            340                 345                 350         


Phe Asn Phe Lys Asp Ile Cys Ala Arg Leu Glu Gln Ala Ser Gly Leu 
        355                 360                 365             


Ile Thr Val Thr Asp Ala Thr Thr Leu Ala Lys Glu Val Ser Ser Leu 
    370                 375                 380                 


Leu Thr Asp Ala Asp Tyr Arg Ser Phe Tyr Gly Arg His Ala Val Glu 
385                 390                 395                 400 


Val Leu Tyr Gln Asn Gln Gly Ala Leu Gln Arg Leu Leu Gln Leu Leu 
                405                 410                 415     


Glu Pro Tyr Leu Pro Pro Lys Thr His 
            420                 425 

<210>  5
<211>  26
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
cgggatccac cagtgaaccg ccaaca                                             26

<210>  6
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
tgcgcggacg taagactc                                                      18

<210>  7
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  7
gagtcttacg tccgcgca                                                      18

<210>  8
<211>  35
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
aaggaaaaaa gcggccgctt cccgcacctt tattg                                   35

<210>  9
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  9
cttcccgaaa gccgattg                                                      18

<210>  10
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  10
acaaaatata ctttaatc                                                      18

<210>  11
<211>  1596
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  11
accagtgaac cgccaacaaa ggcgagatcg gcaatgccat acagtaacat caactcgccc        60
atcgtatcgc caaccacaac ctgcgtgctg gtggagggga cttcccctga agagcgtgtg       120
atatagctta gtccagcctg gcggacaagg ttaatcgcat ccgggaagcg ttccggatga       180
cggggtacca ggatgagcaa taaattcggg aattgctgta acaatgcctg atgtgcggcg       240
atcaccacac tctcttcgcc ttcgtgagtg ctggtggcaa tccataccgg gcggtgtggt       300
gcccactggc ggcgcagcgt cacggcttta gcagccaact gcggcgttac agaaatatcg       360
aatttcaggc taccggtaac ggtcacctga ttattttttg cgcccagcgc cacaaaacgt       420
gcaccatctt cttcattttg cgcagcaatc agcgtaatac gacgcagcaa gcgacggacg       480
aatttaccca gtttggcata acctgcggcc gagcgggcag agagtcgcgc gttagcgatc       540
accagcggaa ttttacgttt atgtagcgcc gcaatcaggt taggccatag ttcggtttcc       600
ataatcaaca ccagtttagg gtcgacttta ttcaggaaac ggttgagtgc atcgggcaga       660
tcatacggca gataaacgtg ctgaacatcc ttcccgaaag ccgattgtac gcgctccgaa       720
ccggttggcg tcatggttgt tacggtaatc ggtaaatcag gataacgatg acgcagcgcg       780
cgcaccaacg ggattgccgc cagagtttca ccgacggaga cggagtgcag cataatgccg       840
cctggtttta gcggatggcg gtaaaaaccg taacgttcac cccagcgttt tcgataggcc       900
ggagacttac gtccgcgcac ccagagccgt atccagatca gcggctgaat aaggtagaga       960
agggcggtgt aaagcaattc gagcatagta aatagctgac ttatggatgt gctggggatt      1020
ctatgtattt agctgtggct ttaccattac ttttcccgtt tttgacttaa atagcttcag      1080
tttggtctga tctgccgcta catcttcatt ttttttgtat ttttatgcga ttcattgaaa      1140
ctcggcccca ttttcaaatc tacataggcc gtactgacat tatcgaaatg ctatttttta      1200
tctatttgat ttttatgatt aaagtatatt ttgtgtataa aaatcattcg ggtcggattg      1260
ctgcgaaaga aatgatacac tagcacgtca aagtaagtgc gttatcagta ttcaggtagc      1320
tgttgagcct ggggcggtag cgtgcttttt tctgcttaac ttaaccagac aatcacacaa      1380
aagagtcgct agtggaaaag ccatttcgaa aaatcctggt cataaagatg cgatatcatg      1440
gggatatgtt attaactact cctgtcatca gtacgctcaa gcagaattat cctgatgcaa      1500
aaatcgatat gctgctttat caggacacca tccctatttt gtctgaaaac ccggaaatta      1560
atgcgctcta tgggataagc aataaaggtg cgggaa                                1596

<210>  12
<211>  544
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  12
cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg ttacggtaat        60
cggtaaatca ggataacgat gacgcagcgc gcgcaccaac gggattgccg ccagagtttc       120
accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc ggtaaaaacc       180
gtaacgttca ccccagcgtt ttcgataggc cggagactta cgtccgcgca cccagagccg       240
tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt cgagcatagt       300
aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc tttaccatta       360
cttttcccgt ttttgactta aatagcttca gtttggtctg atctgccgct acatcttcat       420
tttttttgta tttttatgcg attcattgaa actcggcccc attttcaaat ctacataggc       480
cgtactgaca ttatcgaaat gctatttttt atctatttga tttttatgat taaagtatat       540
tttg                                                                    544

<210>  13
<211>  2109
<212>  DNA
<213>  Escherichia coli

<400>  13
atgtatctgt ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc        60
aagcgtctgc ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca       120
agcggtgaac cctatatcac gcacccggta gcggttgcct gcattctggc cgagatgaaa       180
ctcgactatg aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc       240
acctaccagg atatggaaca gctttttggt aaaagcgtcg ccgagctggt agagggggtg       300
tcgaaacttg ataaactcaa gttccgcgat aagaaagagg cgcaggccga aaactttcgc       360
aagatgatta tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt       420
acccacaaca tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt       480
gaaactctcg aaatttatag cccgctggcg caccgtttag gtatccacca cattaaaacc       540
gaactcgaag agctgggttt tgaggcgctg tatcccaacc gttatcgcgt aatcaaagaa       600
gtggtgaaag ccgcgcgcgg caaccgtaaa gagatgatcc agaagattct ttctgaaatc       660
gaagggcgtt tgcaggaagc gggaataccg tgccgcgtca gtggtcgcga gaagcatctt       720
tattcgattt actgcaaaat ggtgctcaaa gagcagcgtt ttcactcgat catggacatc       780
tacgctttcc gcgtgatcgt caatgattct gacacctgtt atcgcgtgct gggccagatg       840
cacagcctgt acaagccgcg tccgggccgc gtgaaagact atatcgccat tccaaaagcg       900
aacggctatc agtctttgca cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc       960
cagatccgta ccgaagatat ggaccagatg gcggagatgg gtgttgccgc gcactgggct      1020
tataaagagc acggcgaaac cagtactacc gcacaaatcc gcgcccagcg ctggatgcaa      1080
agcctgctgg agctgcaaca gagcgccggt agttcgtttg aatttatcga gagcgttaaa      1140
tccgatctct tcccggatga gatttacgtt ttcacaccgg aagggcgcat tgtcgagctg      1200
cctgccggtg caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc      1260
tgcgtgggcg cacgcgttga ccgccagcct tacccgctgt cgcagccgct taccagcggt      1320
caaaccgttg aaatcattac cgctccgggc gctcgcccga atgccgcttg gctgaacttt      1380
gtcgttagct cgaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat      1440
gattctgtaa gcctgggccg tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg      1500
aatgaaatcc cgcaggaaaa tattcagcgc gagctggatc gcatgaagct ggcaacgctt      1560
gacgatctgc tggcagaaat cggacttggt aacgcaatga gcgtggtggt cgcgaaaaat      1620
ctgcaacatg gggacgcctc cattccaccg gcaacccaaa gccacggaca tctgcccatt      1680
aaaggtgccg atggcgtgct gatcaccttt gcgaaatgct gccgccctat tcctggcgac      1740
ccgattatcg cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt      1800
aatatccgtg gctaccagaa agagccagag aagtttatgg ctgtggaatg ggataaagag      1860
acggcgcagg agttcatcac cgaaatcaag gtggagatgt tcaatcatca gggtgcgctg      1920
gcaaacctga cggcggcaat taacaccacg acttcgaata ttcaaagttt gaatacggaa      1980
gagaaagatg gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga ccgtgtgcat      2040
ctggcgaata tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac      2100
cgaaattaa                                                              2109

<210>  14
<211>  2109
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  14
atgtatctgt ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc        60
aagcgtctgc ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca       120
agcggtgaac cctatatcac gcacccggta gcggttgcct gcattctggc cgagatgaaa       180
ctcgactatg aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc       240
acctaccagg atatggaaca gctttttggt aaaagcgtcg ccgagctggt agagggggtg       300
tcgaaacttg ataaactcaa gttccgcgat aagaaagagg cgcaggccga aaactttcgc       360
aagatgatta tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt       420
acccacaaca tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt       480
gaaactctcg aaatttatag cccgctggcg caccgtttat gtatccacca cattaaaacc       540
gaactcgaag agctgggttt tgaggcgctg tatcccaacc gttatcgcgt aatcaaagaa       600
gtggtgaaag ccgcgcgcgg caaccgtaaa gagatgatcc agaagattct ttctgaaatc       660
gaagggcgtt tgcaggaagc gggaataccg tgccgcgtca gtggtcgcga gaagcatctt       720
tattcgattt actgcaaaat ggtgctcaaa gagcagcgtt ttcactcgat catggacatc       780
tacgctttcc gcgtgatcgt caatgattct gacacctgtt atcgcgtgct gggccagatg       840
cacagcctgt acaagccgcg tccgggccgc gtgaaagact atatcgccat tccaaaagcg       900
aacggctatc agtctttgca cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc       960
cagatccgta ccgaagatat ggaccagatg gcggagatgg gtgttgccgc gcactgggct      1020
tataaagagc acggcgaaac cagtactacc gcacaaatcc gcgcccagcg ctggatgcaa      1080
agcctgctgg agctgcaaca gagcgccggt agttcgtttg aatttatcga gagcgttaaa      1140
tccgatctct tcccggatga gatttacgtt ttcacaccgg aagggcgcat tgtcgagctg      1200
cctgccggtg caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc      1260
tgcgtgggcg cacgcgttga ccgccagcct tacccgctgt cgcagccgct taccagcggt      1320
caaaccgttg aaatcattac cgctccgggc gctcgcccga atgccgcttg gctgaacttt      1380
gtcgttagct cgaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat      1440
gattctgtaa gcctgggccg tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg      1500
aatgaaatcc cgcaggaaaa tattcagcgc gagctggatc gcatgaagct ggcaacgctt      1560
gacgatctgc tggcagaaat cggacttggt aacgcaatga gcgtggtggt cgcgaaaaat      1620
ctgcaacatg gggacgcctc cattccaccg gcaacccaaa gccacggaca tctgcccatt      1680
aaaggtgccg atggcgtgct gatcaccttt gcgaaatgct gccgccctat tcctggcgac      1740
ccgattatcg cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt      1800
aatatccgtg gctaccagaa agagccagag aagtttatgg ctgtggaatg ggataaagag      1860
acggcgcagg agttcatcac cgaaatcaag gtggagatgt tcaatcatca gggtgcgctg      1920
gcaaacctga cggcggcaat taacaccacg acttcgaata ttcaaagttt gaatacggaa      1980
gagaaagatg gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga ccgtgtgcat      2040
ctggcgaata tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac      2100
cgaaattaa                                                              2109

<210>  15
<211>  702
<212>  PRT
<213>  Escherichia coli

<400>  15
Met Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro 
1               5                   10                  15      
Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp 
            20                  25                  30          
Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His 
        35                  40                  45              
Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu 
    50                  55                  60                  
Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala 
65                  70                  75                  80  
Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu 
                85                  90                  95      
Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp Lys Lys 
            100                 105                 110         
Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln 
        115                 120                 125             
Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met 
    130                 135                 140                 
Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg 
145                 150                 155                 160 
Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Gly Ile His 
                165                 170                 175     
His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro 
            180                 185                 190         
Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn 
        195                 200                 205             
Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu 
    210                 215                 220                 
Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu 
225                 230                 235                 240 
Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe His Ser 
                245                 250                 255     
Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Asn Asp Ser Asp Thr 
            260                 265                 270         
Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro Arg Pro 
        275                 280                 285             
Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln 
    290                 295                 300                 
Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val Glu Val 
305                 310                 315                 320 
Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala 
                325                 330                 335     
Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr Ala Gln 
            340                 345                 350         
Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln Gln Ser 
        355                 360                 365             
Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp Leu Phe 
    370                 375                 380                 
Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val Glu Leu 
385                 390                 395                 400 
Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His Thr Asp 
                405                 410                 415     
Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro Tyr Pro 
            420                 425                 430         
Leu Ser Gln Pro Leu Thr Ser Gly Gln Thr Val Glu Ile Ile Thr Ala 
        435                 440                 445             
Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val Ser Ser 
    450                 455                 460                 
Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys Arg Asp 
465                 470                 475                 480 
Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu Gly Gly 
                485                 490                 495     
Ser Arg Lys Leu Asn Glu Ile Pro Gln Glu Asn Ile Gln Arg Glu Leu 
            500                 505                 510         
Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu Ile Gly 
        515                 520                 525             
Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln His Gly 
    530                 535                 540                 
Asp Ala Ser Ile Pro Pro Ala Thr Gln Ser His Gly His Leu Pro Ile 
545                 550                 555                 560 
Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg Pro 
                565                 570                 575     
Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly Leu 
            580                 585                 590         
Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys Glu 
        595                 600                 605             
Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Ala Gln Glu 
    610                 615                 620                 
Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly Ala Leu 
625                 630                 635                 640 
Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile Gln Ser 
                645                 650                 655     
Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe Ile Arg 
            660                 665                 670         
Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg Lys Ile 
        675                 680                 685             
Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn 
    690                 695                 700         

<210>  16
<211>  702
<212>  PRT
<213>  Escherichia coli

<400>  16
Met Tyr Leu Phe Glu Ser Leu Asn Gln Leu Ile Gln Thr Tyr Leu Pro 
1               5                   10                  15      
Glu Asp Gln Ile Lys Arg Leu Arg Gln Ala Tyr Leu Val Ala Arg Asp 
            20                  25                  30          
Ala His Glu Gly Gln Thr Arg Ser Ser Gly Glu Pro Tyr Ile Thr His 
        35                  40                  45              
Pro Val Ala Val Ala Cys Ile Leu Ala Glu Met Lys Leu Asp Tyr Glu 
    50                  55                  60                  
Thr Leu Met Ala Ala Leu Leu His Asp Val Ile Glu Asp Thr Pro Ala 
65                  70                  75                  80  
Thr Tyr Gln Asp Met Glu Gln Leu Phe Gly Lys Ser Val Ala Glu Leu 
                85                  90                  95      
Val Glu Gly Val Ser Lys Leu Asp Lys Leu Lys Phe Arg Asp Lys Lys 
            100                 105                 110         
Glu Ala Gln Ala Glu Asn Phe Arg Lys Met Ile Met Ala Met Val Gln 
        115                 120                 125             
Asp Ile Arg Val Ile Leu Ile Lys Leu Ala Asp Arg Thr His Asn Met 
    130                 135                 140                 
Arg Thr Leu Gly Ser Leu Arg Pro Asp Lys Arg Arg Arg Ile Ala Arg 
145                 150                 155                 160 
Glu Thr Leu Glu Ile Tyr Ser Pro Leu Ala His Arg Leu Cys Ile His 
                165                 170                 175     
His Ile Lys Thr Glu Leu Glu Glu Leu Gly Phe Glu Ala Leu Tyr Pro 
            180                 185                 190         
Asn Arg Tyr Arg Val Ile Lys Glu Val Val Lys Ala Ala Arg Gly Asn 
        195                 200                 205             
Arg Lys Glu Met Ile Gln Lys Ile Leu Ser Glu Ile Glu Gly Arg Leu 
    210                 215                 220                 
Gln Glu Ala Gly Ile Pro Cys Arg Val Ser Gly Arg Glu Lys His Leu 
225                 230                 235                 240 
Tyr Ser Ile Tyr Cys Lys Met Val Leu Lys Glu Gln Arg Phe His Ser 
                245                 250                 255     
Ile Met Asp Ile Tyr Ala Phe Arg Val Ile Val Asn Asp Ser Asp Thr 
            260                 265                 270         
Cys Tyr Arg Val Leu Gly Gln Met His Ser Leu Tyr Lys Pro Arg Pro 
        275                 280                 285             
Gly Arg Val Lys Asp Tyr Ile Ala Ile Pro Lys Ala Asn Gly Tyr Gln 
    290                 295                 300                 
Ser Leu His Thr Ser Met Ile Gly Pro His Gly Val Pro Val Glu Val 
305                 310                 315                 320 
Gln Ile Arg Thr Glu Asp Met Asp Gln Met Ala Glu Met Gly Val Ala 
                325                 330                 335     
Ala His Trp Ala Tyr Lys Glu His Gly Glu Thr Ser Thr Thr Ala Gln 
            340                 345                 350         
Ile Arg Ala Gln Arg Trp Met Gln Ser Leu Leu Glu Leu Gln Gln Ser 
        355                 360                 365             
Ala Gly Ser Ser Phe Glu Phe Ile Glu Ser Val Lys Ser Asp Leu Phe 
    370                 375                 380                 
Pro Asp Glu Ile Tyr Val Phe Thr Pro Glu Gly Arg Ile Val Glu Leu 
385                 390                 395                 400 
Pro Ala Gly Ala Thr Pro Val Asp Phe Ala Tyr Ala Val His Thr Asp 
                405                 410                 415     
Ile Gly His Ala Cys Val Gly Ala Arg Val Asp Arg Gln Pro Tyr Pro 
            420                 425                 430         
Leu Ser Gln Pro Leu Thr Ser Gly Gln Thr Val Glu Ile Ile Thr Ala 
        435                 440                 445             
Pro Gly Ala Arg Pro Asn Ala Ala Trp Leu Asn Phe Val Val Ser Ser 
    450                 455                 460                 
Lys Ala Arg Ala Lys Ile Arg Gln Leu Leu Lys Asn Leu Lys Arg Asp 
465                 470                 475                 480 
Asp Ser Val Ser Leu Gly Arg Arg Leu Leu Asn His Ala Leu Gly Gly 
                485                 490                 495     
Ser Arg Lys Leu Asn Glu Ile Pro Gln Glu Asn Ile Gln Arg Glu Leu 
            500                 505                 510         
Asp Arg Met Lys Leu Ala Thr Leu Asp Asp Leu Leu Ala Glu Ile Gly 
        515                 520                 525             
Leu Gly Asn Ala Met Ser Val Val Val Ala Lys Asn Leu Gln His Gly 
    530                 535                 540                 
Asp Ala Ser Ile Pro Pro Ala Thr Gln Ser His Gly His Leu Pro Ile 
545                 550                 555                 560 
Lys Gly Ala Asp Gly Val Leu Ile Thr Phe Ala Lys Cys Cys Arg Pro 
                565                 570                 575     
Ile Pro Gly Asp Pro Ile Ile Ala His Val Ser Pro Gly Lys Gly Leu 
            580                 585                 590         
Val Ile His His Glu Ser Cys Arg Asn Ile Arg Gly Tyr Gln Lys Glu 
        595                 600                 605             
Pro Glu Lys Phe Met Ala Val Glu Trp Asp Lys Glu Thr Ala Gln Glu 
    610                 615                 620                 
Phe Ile Thr Glu Ile Lys Val Glu Met Phe Asn His Gln Gly Ala Leu 
625                 630                 635                 640 
Ala Asn Leu Thr Ala Ala Ile Asn Thr Thr Thr Ser Asn Ile Gln Ser 
                645                 650                 655     
Leu Asn Thr Glu Glu Lys Asp Gly Arg Val Tyr Ser Ala Phe Ile Arg 
            660                 665                 670         
Leu Thr Ala Arg Asp Arg Val His Leu Ala Asn Ile Met Arg Lys Ile 
        675                 680                 685             
Arg Val Met Pro Asp Val Ile Lys Val Thr Arg Asn Arg Asn 
    690                 695                 700         

<210>  17
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  17
cgggatccga acagcaagag caggaagc                                           28

<210>  18
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  18
tgtggtggat acataaacg                                                     19

<210>  19
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  19
gcaccgttta tgtatccacc                                                    20

<210>  20
<211>  38
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  20
aaggaaaaaa gcggccgcac gacaaagttc agccaagc                                38

<210>  21
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  21
ctttcgcaag atgattatgg                                                    20

<210>  22
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  22
cacggtattc ccgcttcctg                                                    20

<210>  23
<211>  567
<212>  DNA
<213>  Escherichia coli

<400>  23
atgaatatca ctgctactgt tcttcttgcg tttggtatgt cgatggatgc atttgctgca        60
tcaatcggta aaggtgccac cctccataaa ccgaaatttt ctgaagcatt gcgaaccggc       120
cttatttttg gtgccgtcga aaccctgacg ccgctgatcg gctggggaat gggcatgtta       180
gccagccggt ttgtccttga atggaaccac tggattgcgt ttgtgctgct gatattcctc       240
ggcgggcgaa tgattattga gggttttcgt ggcgcagatg atgaagatga agagccgcgc       300
cgtcgacacg gtttctggct actggtaacc accgcgattg ccaccagcct ggatgccatg       360
gctgtgggtg ttggtcttgc tttcctgcag gtcaacatta tcgcgaccgc attggccatt       420
ggttgtgcaa ccttgattat gtcaacatta gggatgatgg ttggtcgctt tatcggctca       480
attattggga aaaaagcgga aattctcggc gggctggtgc tgatcggcat cggcgtccag       540
atcctctgga cgcacttcca cggttaa                                           567

<210>  24
<211>  567
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  24
atgaatatca ctgctactgt tcttcttgcg tttggtatgt cgatggatgc atttgctgca        60
tcaatcggta aagatgccac cctccataaa ccgaaatttt ctgaagcatt gcgaaccggc       120
cttatttttg gtgccgtcga aaccctgacg ccgctgatcg gctggggaat gggcatgtta       180
gccagccggt ttgtccttga atggaaccac tggattgcgt ttgtgctgct gatattcctc       240
ggcgggcgaa tgattattga gggttttcgt ggcgcagatg atgaagatga agagccgcgc       300
cgtcgacacg gtttctggct actggtaacc accgcgattg ccaccagcct ggatgccatg       360
gctgtgggtg ttggtcttgc tttcctgcag gtcaacatta tcgcgaccgc attggccatt       420
ggttgtgcaa ccttgattat gtcaacatta gggatgatgg ttggtcgctt tatcggctca       480
attattggga aaaaagcgga aattctcggc gggctggtgc tgatcggcat cggcgtccag       540
atcctctgga cgcacttcca cggttaa                                           567

<210>  25
<211>  188
<212>  PRT
<213>  Escherichia coli

<400>  25
Met Asn Ile Thr Ala Thr Val Leu Leu Ala Phe Gly Met Ser Met Asp 
1               5                   10                  15      
Ala Phe Ala Ala Ser Ile Gly Lys Gly Ala Thr Leu His Lys Pro Lys 
            20                  25                  30          
Phe Ser Glu Ala Leu Arg Thr Gly Leu Ile Phe Gly Ala Val Glu Thr 
        35                  40                  45              
Leu Thr Pro Leu Ile Gly Trp Gly Met Gly Met Leu Ala Ser Arg Phe 
    50                  55                  60                  
Val Leu Glu Trp Asn His Trp Ile Ala Phe Val Leu Leu Ile Phe Leu 
65                  70                  75                  80  
Gly Gly Arg Met Ile Ile Glu Gly Phe Arg Gly Ala Asp Asp Glu Asp 
                85                  90                  95      
Glu Glu Pro Arg Arg Arg His Gly Phe Trp Leu Leu Val Thr Thr Ala 
            100                 105                 110         
Ile Ala Thr Ser Leu Asp Ala Met Ala Val Gly Val Gly Leu Ala Phe 
        115                 120                 125             
Leu Gln Val Asn Ile Ile Ala Thr Ala Leu Ala Ile Gly Cys Ala Thr 
    130                 135                 140                 
Leu Ile Met Ser Thr Leu Gly Met Met Val Gly Arg Phe Ile Gly Ser 
145                 150                 155                 160 
Ile Ile Gly Lys Lys Ala Glu Ile Leu Gly Gly Leu Val Leu Ile Gly 
                165                 170                 175     
Ile Gly Val Gln Ile Leu Trp Thr His Phe His Gly 
            180                 185             

<210>  26
<211>  188
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  26
Met Asn Ile Thr Ala Thr Val Leu Leu Ala Phe Gly Met Ser Met Asp 
1               5                   10                  15      
Ala Phe Ala Ala Ser Ile Gly Lys Asp Ala Thr Leu His Lys Pro Lys 
            20                  25                  30          
Phe Ser Glu Ala Leu Arg Thr Gly Leu Ile Phe Gly Ala Val Glu Thr 
        35                  40                  45              
Leu Thr Pro Leu Ile Gly Trp Gly Met Gly Met Leu Ala Ser Arg Phe 
    50                  55                  60                  
Val Leu Glu Trp Asn His Trp Ile Ala Phe Val Leu Leu Ile Phe Leu 
65                  70                  75                  80  
Gly Gly Arg Met Ile Ile Glu Gly Phe Arg Gly Ala Asp Asp Glu Asp 
                85                  90                  95      
Glu Glu Pro Arg Arg Arg His Gly Phe Trp Leu Leu Val Thr Thr Ala 
            100                 105                 110         
Ile Ala Thr Ser Leu Asp Ala Met Ala Val Gly Val Gly Leu Ala Phe 
        115                 120                 125             
Leu Gln Val Asn Ile Ile Ala Thr Ala Leu Ala Ile Gly Cys Ala Thr 
    130                 135                 140                 
Leu Ile Met Ser Thr Leu Gly Met Met Val Gly Arg Phe Ile Gly Ser 
145                 150                 155                 160 
Ile Ile Gly Lys Lys Ala Glu Ile Leu Gly Gly Leu Val Leu Ile Gly 
                165                 170                 175     
Ile Gly Val Gln Ile Leu Trp Thr His Phe His Gly 
            180                 185             

<210>  27
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  27
cgggatccct tcgccaatgt ctggattg                                           28

<210>  28
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  28
atggagggtg gcatctttac                                                    20

<210>  29
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  29
tgcatcaatc ggtaaagatg                                                    20

<210>  30
<211>  38
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  30
aaggaaaaaa gcggccgcca actccgcact ctgctgta                                38

<210>  31
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  31
ccatcacggc ttgttgttc                                                     19

<210>  32
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  32
acgaaaaccc tcaataatc                                                     19


