﻿                         序列表

<110>  深圳宾德生物技术有限公司

<120>  靶向Her2并干扰IL-6表达的嵌合抗原受体T细胞及其制备方法和应用

<150>  CN201910792817.X
<151>  2019-08-26

<160>  16

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  240
<212>  PRT
<213>  Artificial Sequence

<400>  1
Asp Ile Gln Leu Thr Gln Ser His Lys Phe Leu Ser Thr Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Tyr Asn Ala 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Ser Arg Tyr Thr Gly Val Pro Ser Arg Phe Thr Gly 
    50                  55                  60                  
Ser Gly Ser Gly Pro Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  
Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln His Phe Arg Thr Pro Phe 
                85                  90                  95      
Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln Ser 
        115                 120                 125             
Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys 
    130                 135                 140                 
Ala Ser Gly Tyr Pro Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln 
145                 150                 155                 160 
Ala Pro Gly Gln Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Ser Thr 
                165                 170                 175     
Gly Glu Ser Thr Phe Ala Asp Asp Phe Lys Gly Arg Phe Asp Phe Ser 
            180                 185                 190         
Leu Glu Thr Ser Ala Asn Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys 
        195                 200                 205             
Ser Glu Asp Met Ala Thr Tyr Phe Cys Ala Arg Trp Glu Val Tyr His 
    210                 215                 220                 
Gly Tyr Val Pro Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 

<210>  2
<211>  23
<212>  DNA
<213>  Artificial Sequence

<400>  2
acctcagatt gttgttgtta atg                                                23

<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<400>  3
aagctgagtt aatttatgta agt                                                23

<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<400>  4
ttggagtttg aggtatacct aga                                                23

<210>  5
<211>  463
<212>  PRT
<213>  Artificial Sequence

<400>  5
Asp Ile Gln Leu Thr Gln Ser His Lys Phe Leu Ser Thr Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Tyr Asn Ala 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Ser Arg Tyr Thr Gly Val Pro Ser Arg Phe Thr Gly 
    50                  55                  60                  
Ser Gly Ser Gly Pro Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  
Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln His Phe Arg Thr Pro Phe 
                85                  90                  95      
Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln Ser 
        115                 120                 125             
Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys 
    130                 135                 140                 
Ala Ser Gly Tyr Pro Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln 
145                 150                 155                 160 
Ala Pro Gly Gln Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Ser Thr 
                165                 170                 175     
Gly Glu Ser Thr Phe Ala Asp Asp Phe Lys Gly Arg Phe Asp Phe Ser 
            180                 185                 190         
Leu Glu Thr Ser Ala Asn Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys 
        195                 200                 205             
Ser Glu Asp Met Ala Thr Tyr Phe Cys Ala Arg Trp Glu Val Tyr His 
    210                 215                 220                 
Gly Tyr Val Pro Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
                245                 250                 255     
Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            260                 265                 270         
Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        275                 280                 285             
Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    290                 295                 300                 
Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
305                 310                 315                 320 
Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                325                 330                 335     
Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
            340                 345                 350         
Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln 
        355                 360                 365             
Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    370                 375                 380                 
Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
385                 390                 395                 400 
Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                405                 410                 415     
Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            420                 425                 430         
Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        435                 440                 445             
Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460             

<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  6
ttaacaacaa caatctgagg t                                                  21

<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  7
ctcagattgt tgttgttaat g                                                  21

<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  8
gctgagttaa tttatgtaag t                                                  21

<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  9
ttacataaat taactcagct t                                                  21

<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  10
taggtatacc tcaaactcca a                                                  21

<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  11
ggagtttgag gtatacctag a                                                  21

<210>  12
<211>  720
<212>  DNA
<213>  Artificial Sequence

<400>  12
gacatccagc tgacccagtc tcacaaattc ctgtccactt cagtaggaga cagggtcagc        60
atcacctgca aggccagtca ggatgtgtat aatgctgttg cctggtatca acagaaacca       120
ggacaatctc ctaaacttct gatttactcg gcatcctccc ggtacactgg agtcccttct       180
cgcttcactg gcagtggctc tgggccggat ttcactttca ccatcagcag tgtgcaggct       240
gaagacctgg cagtttattt ctgtcagcaa cattttcgta ctccattcac gttcggctcg       300
gggacaaaat tggagatcgg cggtggcggt tctggtggcg gtggctccgg cggtggcggt       360
tctcaggtac aactgcagca gtctggacct gaactgaaga agcctggaga gacagtcaag       420
atctcctgca aggcctctgg gtatcctttc acaaactatg gaatgaactg ggtgaagcag       480
gctccaggac agggtttaaa gtggatgggc tggattaaca cttccactgg agagtcaaca       540
tttgctgatg acttcaaggg acggtttgac ttctctttgg aaacctctgc caacactgcc       600
tatttgcaga tcaacaacct caaaagtgaa gacatggcta catatttctg tgcaagatgg       660
gaggtttacc acggctacgt tccttactgg ggccaaggga ccacggtcac cgtttcctct       720

<210>  13
<211>  1389
<212>  DNA
<213>  Artificial Sequence

<400>  13
gacatccagc tgacccagtc tcacaaattc ctgtccactt cagtaggaga cagggtcagc        60
atcacctgca aggccagtca ggatgtgtat aatgctgttg cctggtatca acagaaacca       120
ggacaatctc ctaaacttct gatttactcg gcatcctccc ggtacactgg agtcccttct       180
cgcttcactg gcagtggctc tgggccggat ttcactttca ccatcagcag tgtgcaggct       240
gaagacctgg cagtttattt ctgtcagcaa cattttcgta ctccattcac gttcggctcg       300
gggacaaaat tggagatcgg cggtggcggt tctggtggcg gtggctccgg cggtggcggt       360
tctcaggtac aactgcagca gtctggacct gaactgaaga agcctggaga gacagtcaag       420
atctcctgca aggcctctgg gtatcctttc acaaactatg gaatgaactg ggtgaagcag       480
gctccaggac agggtttaaa gtggatgggc tggattaaca cttccactgg agagtcaaca       540
tttgctgatg acttcaaggg acggtttgac ttctctttgg aaacctctgc caacactgcc       600
tatttgcaga tcaacaacct caaaagtgaa gacatggcta catatttctg tgcaagatgg       660
gaggtttacc acggctacgt tccttactgg ggccaaggga ccacggtcac cgtttcctct       720
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       780
tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg       840
gacttcgcct gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc       900
ctgtcactgg ttatcaccct ttactgcaaa cggggcagaa agaaactcct gtatatattc       960
aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga      1020
tttccagaag aagaagaagg aggatgtgaa ctgagagtga agttcagcag gagcgcagac      1080
gcccccgcgt acaagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga      1140
gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg      1200
agaaggaaga accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag      1260
gcctacagtg agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt      1320
taccagggtc tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg      1380
ccccctcgc                                                              1389

<210>  14
<211>  20
<212>  PRT
<213>  Artificial Sequence

<400>  14
Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His 
1               5                   10                  15      
Ala Ala Arg Pro 
            20  

<210>  15
<211>  483
<212>  PRT
<213>  Artificial Sequence

<400>  15
Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His 
1               5                   10                  15      
Ala Ala Arg Pro Asp Ile Gln Leu Thr Gln Ser His Lys Phe Leu Ser 
            20                  25                  30          
Thr Ser Val Gly Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp 
        35                  40                  45              
Val Tyr Asn Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro 
    50                  55                  60                  
Lys Leu Leu Ile Tyr Ser Ala Ser Ser Arg Tyr Thr Gly Val Pro Ser 
65                  70                  75                  80  
Arg Phe Thr Gly Ser Gly Ser Gly Pro Asp Phe Thr Phe Thr Ile Ser 
                85                  90                  95      
Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln His Phe 
            100                 105                 110         
Arg Thr Pro Phe Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Gly Gly 
        115                 120                 125             
Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
    130                 135                 140                 
Leu Gln Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr Val Lys 
145                 150                 155                 160 
Ile Ser Cys Lys Ala Ser Gly Tyr Pro Phe Thr Asn Tyr Gly Met Asn 
                165                 170                 175     
Trp Val Lys Gln Ala Pro Gly Gln Gly Leu Lys Trp Met Gly Trp Ile 
            180                 185                 190         
Asn Thr Ser Thr Gly Glu Ser Thr Phe Ala Asp Asp Phe Lys Gly Arg 
        195                 200                 205             
Phe Asp Phe Ser Leu Glu Thr Ser Ala Asn Thr Ala Tyr Leu Gln Ile 
    210                 215                 220                 
Asn Asn Leu Lys Ser Glu Asp Met Ala Thr Tyr Phe Cys Ala Arg Trp 
225                 230                 235                 240 
Glu Val Tyr His Gly Tyr Val Pro Tyr Trp Gly Gln Gly Thr Thr Val 
                245                 250                 255     
Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
            260                 265                 270         
Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
        275                 280                 285             
Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
    290                 295                 300                 
Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu 
305                 310                 315                 320 
Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu 
                325                 330                 335     
Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
            340                 345                 350         
Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
        355                 360                 365             
Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
    370                 375                 380                 
Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
385                 390                 395                 400 
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
                405                 410                 415     
Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
            420                 425                 430         
Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
        435                 440                 445             
Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
    450                 455                 460                 
Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
465                 470                 475                 480 
Pro Pro Arg 
            

<210>  16
<211>  419
<212>  DNA
<213>  Artificial Sequence

<400>  16
tcccccgggt tatagggagc tgaagggaag ggggtcacag taggtggcat cgttcctttc        60
tgactgcccg ccccccgcat gccgtcccgc gatattgagc tccgaacctc tcgccctgcc       120
gccgccggtg ctccgtcgcc gccgcgccgc catggaattc gaacgctgac gtcatcaacc       180
cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc       240
tggcaggaag atggctgtga gggacagggg agtggcgccc tgcaatattt gcatgtcgct       300
atgtgttctg ggaaatcacc ataaacgtga aatgtctttg gatttgggaa tcttataagt       360
tctgtatgag accactcttt cccacctcag attgttgttg ttaatgcata tggaattcc        419
