﻿                         序列表

<110>  深圳宾德生物技术有限公司

<120>  靶向EGFRvⅢ并干扰IL-6表达的嵌合抗原受体T细胞及其制备方法和应用

<150>  CN201910792807.6
<151>  2019-08-26

<160>  16

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  246
<212>  PRT
<213>  Artificial Sequence

<400>  1
Gly Ile Gly Leu Val Gly Ser Gly Ala Gly Val Leu Leu Pro Gly Gly 
1               5                   10                  15      
Ser Leu Ala Ile Ser Cys Leu Gly Ser Gly Pro Ala Ile Gly Ala Thr 
            20                  25                  30          
Thr Ile His Thr Val Ala Gly Met Pro Gly Leu Gly Leu Gly Thr Met 
        35                  40                  45              
Gly Ala Ile Ala Pro Gly Ala Ala Gly Thr Leu Thr Gly Pro Ile Pro 
    50                  55                  60                  
Gly Gly His Val Thr Ile Ser Ala Ala Thr Ser Ile Ala Thr Val Thr 
65                  70                  75                  80  
Leu Gly Thr Ser Ser Leu Leu Ala Ser Ala Thr Ala Met Thr Thr Cys 
                85                  90                  95      
Ala Pro Ala Gly Gly Val Thr Thr Gly Gly Gly Thr Thr Val Thr Val 
            100                 105                 110         
Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Ala Val Val Met Thr Gly Ser Pro Ala Ser 
    130                 135                 140                 
Leu Ala Val Ser Leu Gly Gly Ala Ala Thr Ile Ala Cys Leu Ser Ser 
145                 150                 155                 160 
Gly Ser Leu Leu Ala Ser Ala Gly Leu Thr Thr Leu Ala Thr Leu Gly 
                165                 170                 175     
Gly Leu Pro Gly Gly Pro Pro Leu Ala Leu Ile Ser Leu Val Ser Leu 
            180                 185                 190         
Leu Ala Ser Gly Val Pro Ala Ala Pro Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             
Ala Pro Thr Leu Thr Ile Ser Ser Leu Gly Ala Gly Ala Val Ala Val 
    210                 215                 220                 
Thr Thr Cys Thr Gly Gly Thr His Pro Pro Gly Thr Pro Gly Gly Gly 
225                 230                 235                 240 
Thr Leu Val Gly Ile Leu 
                245     

<210>  2
<211>  23
<212>  DNA
<213>  Artificial Sequence

<400>  2
acctcagatt gttgttgtta atg                                                23

<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<400>  3
aagctgagtt aatttatgta agt                                                23

<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<400>  4
ttggagtttg aggtatacct aga                                                23

<210>  5
<211>  469
<212>  PRT
<213>  Artificial Sequence

<400>  5
Glu Ile Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      
Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Phe Asn Ile Glu Asp Tyr 
            20                  25                  30          
Tyr Ile His Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              
Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr Lys Tyr Gly Pro Ile Phe 
    50                  55                  60                  
Gln Gly His Val Thr Ile Ser Ala Asp Thr Ser Ile Asn Thr Val Tyr 
65                  70                  75                  80  
Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      
Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln Gly Thr Thr Val Thr Val 
            100                 105                 110         
Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln Ser Pro Asp Ser 
    130                 135                 140                 
Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser 
145                 150                 155                 160 
Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu Asn Trp Leu Gln 
                165                 170                 175     
Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu Ile Ser Leu Val Ser Lys 
            180                 185                 190         
Leu Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr 
        195                 200                 205             
Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val 
    210                 215                 220                 
Tyr Tyr Cys Trp Gln Gly Thr His Phe Pro Gly Thr Phe Gly Gly Gly 
225                 230                 235                 240 
Thr Lys Val Glu Ile Lys Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr 
                245                 250                 255     
Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala 
            260                 265                 270         
Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe 
        275                 280                 285             
Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val 
    290                 295                 300                 
Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys 
305                 310                 315                 320 
Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
                325                 330                 335     
Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
            340                 345                 350         
Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
        355                 360                 365             
Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
    370                 375                 380                 
Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
385                 390                 395                 400 
Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
                405                 410                 415     
Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
            420                 425                 430         
Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
        435                 440                 445             
Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
    450                 455                 460                 
Ala Leu Pro Pro Arg 
465                 

<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  6
ttaacaacaa caatctgagg t                                                  21

<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  7
ctcagattgt tgttgttaat g                                                  21

<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  8
gctgagttaa tttatgtaag t                                                  21

<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  9
ttacataaat taactcagct t                                                  21

<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  10
taggtatacc tcaaactcca a                                                  21

<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<400>  11
ggagtttgag gtatacctag a                                                  21

<210>  12
<211>  738
<212>  DNA
<213>  Artificial Sequence

<400>  12
gagattcagc tcgtgcaatc gggagcggaa gtcaagaagc caggagagtc cttgcggatc        60
tcatgcaagg gtagcggctt taacatcgag gattactaca tccactgggt gaggcagatg       120
ccggggaagg gactcgaatg gatgggacgg atcgacccag aaaacgacga aactaagtac       180
ggtccgatct tccaaggcca tgtgactatt agcgccgata cttcaatcaa taccgtgtat       240
ctgcaatggt cctcattgaa agcctcagat accgcgatgt actactgtgc tttcagagga       300
ggggtctact ggggacaggg aactaccgtg actgtctcgt ccggcggagg cgggtcagga       360
ggtggcggca gcggaggagg agggtccggc ggaggtgggt ccgacgtcgt gatgacccag       420
agccctgaca gcctggcagt gagcctgggc gaaagagcta ccattaactg caaatcgtcg       480
cagagcctgc tggactcgga cggaaaaacg tacctcaatt ggctgcagca aaagcctggc       540
cagccaccga agcgccttat ctcactggtg tcgaagctgg attcgggagt gcccgatcgc       600
ttctccggct cgggatcggg tactgacttc accctcacta tctcctcgct tcaagcagag       660
gacgtggccg tctactactg ctggcaggga acccactttc cgggaacctt cggcggaggg       720
acgaaagtgg agatcaag                                                     738

<210>  13
<211>  1407
<212>  DNA
<213>  Artificial Sequence

<400>  13
gagattcagc tcgtgcaatc gggagcggaa gtcaagaagc caggagagtc cttgcggatc        60
tcatgcaagg gtagcggctt taacatcgag gattactaca tccactgggt gaggcagatg       120
ccggggaagg gactcgaatg gatgggacgg atcgacccag aaaacgacga aactaagtac       180
ggtccgatct tccaaggcca tgtgactatt agcgccgata cttcaatcaa taccgtgtat       240
ctgcaatggt cctcattgaa agcctcagat accgcgatgt actactgtgc tttcagagga       300
ggggtctact ggggacaggg aactaccgtg actgtctcgt ccggcggagg cgggtcagga       360
ggtggcggca gcggaggagg agggtccggc ggaggtgggt ccgacgtcgt gatgacccag       420
agccctgaca gcctggcagt gagcctgggc gaaagagcta ccattaactg caaatcgtcg       480
cagagcctgc tggactcgga cggaaaaacg tacctcaatt ggctgcagca aaagcctggc       540
cagccaccga agcgccttat ctcactggtg tcgaagctgg attcgggagt gcccgatcgc       600
ttctccggct cgggatcggg tactgacttc accctcacta tctcctcgct tcaagcagag       660
gacgtggccg tctactactg ctggcaggga acccactttc cgggaacctt cggcggaggg       720
acgaaagtgg agatcaagac cacgacgcca gcgccgcgac caccaacacc ggcgcccacc       780
atcgcgtcgc agcccctgtc cctgcgccca gaggcgtgcc ggccagcggc ggggggcgca       840
gtgcacacga gggggctgga cttcgcctgt gatatctaca tctgggcgcc cttggccggg       900
acttgtgggg tccttctcct gtcactggtt atcacccttt actgcaaacg gggcagaaag       960
aaactcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa      1020
gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact gagagtgaag      1080
ttcagcagga gcgcagacgc ccccgcgtac aagcagggcc agaaccagct ctataacgag      1140
ctcaatctag gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct      1200
gagatggggg gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag      1260
aaagataaga tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc      1320
aaggggcacg atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc      1380
cttcacatgc aggccctgcc ccctcgc                                          1407

<210>  14
<211>  20
<212>  PRT
<213>  Artificial Sequence

<400>  14
Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His 
1               5                   10                  15      
Ala Ala Arg Pro 
            20  

<210>  15
<211>  489
<212>  PRT
<213>  Artificial Sequence

<400>  15
Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu His 
1               5                   10                  15      
Ala Ala Arg Pro Glu Ile Gln Leu Val Gln Ser Gly Ala Glu Val Lys 
            20                  25                  30          
Lys Pro Gly Glu Ser Leu Arg Ile Ser Cys Lys Gly Ser Gly Phe Asn 
        35                  40                  45              
Ile Glu Asp Tyr Tyr Ile His Trp Val Arg Gln Met Pro Gly Lys Gly 
    50                  55                  60                  
Leu Glu Trp Met Gly Arg Ile Asp Pro Glu Asn Asp Glu Thr Lys Tyr 
65                  70                  75                  80  
Gly Pro Ile Phe Gln Gly His Val Thr Ile Ser Ala Asp Thr Ser Ile 
                85                  90                  95      
Asn Thr Val Tyr Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala 
            100                 105                 110         
Met Tyr Tyr Cys Ala Phe Arg Gly Gly Val Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             
Thr Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Val Val Met Thr Gln 
145                 150                 155                 160 
Ser Pro Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn 
                165                 170                 175     
Cys Lys Ser Ser Gln Ser Leu Leu Asp Ser Asp Gly Lys Thr Tyr Leu 
            180                 185                 190         
Asn Trp Leu Gln Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu Ile Ser 
        195                 200                 205             
Leu Val Ser Lys Leu Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
    210                 215                 220                 
Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu 
225                 230                 235                 240 
Asp Val Ala Val Tyr Tyr Cys Trp Gln Gly Thr His Phe Pro Gly Thr 
                245                 250                 255     
Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Thr Thr Thr Pro Ala Pro 
            260                 265                 270         
Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
        275                 280                 285             
Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
    290                 295                 300                 
Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
305                 310                 315                 320 
Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys 
                325                 330                 335     
Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
            340                 345                 350         
Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
        355                 360                 365             
Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser 
    370                 375                 380                 
Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
385                 390                 395                 400 
Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
                405                 410                 415     
Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
            420                 425                 430         
Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
        435                 440                 445             
Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
    450                 455                 460                 
Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
465                 470                 475                 480 
Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 

<210>  16
<211>  419
<212>  DNA
<213>  Artificial Sequence

<400>  16
tcccccgggt tatagggagc tgaagggaag ggggtcacag taggtggcat cgttcctttc        60
tgactgcccg ccccccgcat gccgtcccgc gatattgagc tccgaacctc tcgccctgcc       120
gccgccggtg ctccgtcgcc gccgcgccgc catggaattc gaacgctgac gtcatcaacc       180
cgctccaagg aatcgcgggc ccagtgtcac taggcgggaa cacccagcgc gcgtgcgccc       240
tggcaggaag atggctgtga gggacagggg agtggcgccc tgcaatattt gcatgtcgct       300
atgtgttctg ggaaatcacc ataaacgtga aatgtctttg gatttgggaa tcttataagt       360
tctgtatgag accactcttt cccacctcag attgttgttg ttaatgcata tggaattcc        419

