﻿                         序列表

<110>  深圳宾德生物技术有限公司

<120>  携带安全开关并靶向Her2的嵌合抗原受体T细胞及其制备方法和应用

<150>  CN201910792809.5
<151>  2019-08-26

<160>  12

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  240
<212>  PRT
<213>  Artificial Sequence

<400>  1
Asp Ile Gln Leu Thr Gln Ser His Lys Phe Leu Ser Thr Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Tyr Asn Ala 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Ser Arg Tyr Thr Gly Val Pro Ser Arg Phe Thr Gly 
    50                  55                  60                  
Ser Gly Ser Gly Pro Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  
Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln His Phe Arg Thr Pro Phe 
                85                  90                  95      
Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln Ser 
        115                 120                 125             
Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys 
    130                 135                 140                 
Ala Ser Gly Tyr Pro Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln 
145                 150                 155                 160 
Ala Pro Gly Gln Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Ser Thr 
                165                 170                 175     
Gly Glu Ser Thr Phe Ala Asp Asp Phe Lys Gly Arg Phe Asp Phe Ser 
            180                 185                 190         
Leu Glu Thr Ser Ala Asn Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys 
        195                 200                 205             
Ser Glu Asp Met Ala Thr Tyr Phe Cys Ala Arg Trp Glu Val Tyr His 
    210                 215                 220                 
Gly Tyr Val Pro Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 

<210>  2
<211>  107
<212>  PRT
<213>  Artificial Sequence

<400>  2
Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      
Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          
Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              
Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  
Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  
Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      
Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         

<210>  3
<211>  277
<212>  PRT
<213>  Artificial Sequence

<400>  3
Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu 
1               5                   10                  15      
Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe 
            20                  25                  30          
Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys 
        35                  40                  45              
Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met Val Glu Val 
    50                  55                  60                  
Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu 
65                  70                  75                  80  
Ala Gln Gln Asp His Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu 
                85                  90                  95      
Ser His Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr 
            100                 105                 110         
Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe 
        115                 120                 125             
Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe 
    130                 135                 140                 
Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala 
145                 150                 155                 160 
Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp 
                165                 170                 175     
Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala 
            180                 185                 190         
Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr 
        195                 200                 205             
Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr 
    210                 215                 220                 
Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp 
225                 230                 235                 240 
Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly 
                245                 250                 255     
Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu 
            260                 265                 270         
Phe Phe Lys Thr Ser 
        275         

<210>  4
<211>  463
<212>  PRT
<213>  Artificial Sequence

<400>  4
Asp Ile Gln Leu Thr Gln Ser His Lys Phe Leu Ser Thr Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Ser Ile Thr Cys Lys Ala Ser Gln Asp Val Tyr Asn Ala 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Ser Arg Tyr Thr Gly Val Pro Ser Arg Phe Thr Gly 
    50                  55                  60                  
Ser Gly Ser Gly Pro Asp Phe Thr Phe Thr Ile Ser Ser Val Gln Ala 
65                  70                  75                  80  
Glu Asp Leu Ala Val Tyr Phe Cys Gln Gln His Phe Arg Thr Pro Phe 
                85                  90                  95      
Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Gly Gly Gly Gly Ser Gly 
            100                 105                 110         
Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Gln Ser 
        115                 120                 125             
Gly Pro Glu Leu Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys 
    130                 135                 140                 
Ala Ser Gly Tyr Pro Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln 
145                 150                 155                 160 
Ala Pro Gly Gln Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Ser Thr 
                165                 170                 175     
Gly Glu Ser Thr Phe Ala Asp Asp Phe Lys Gly Arg Phe Asp Phe Ser 
            180                 185                 190         
Leu Glu Thr Ser Ala Asn Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys 
        195                 200                 205             
Ser Glu Asp Met Ala Thr Tyr Phe Cys Ala Arg Trp Glu Val Tyr His 
    210                 215                 220                 
Gly Tyr Val Pro Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
225                 230                 235                 240 
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
                245                 250                 255     
Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            260                 265                 270         
Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        275                 280                 285             
Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    290                 295                 300                 
Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
305                 310                 315                 320 
Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
                325                 330                 335     
Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
            340                 345                 350         
Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln 
        355                 360                 365             
Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
    370                 375                 380                 
Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
385                 390                 395                 400 
Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
                405                 410                 415     
Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
            420                 425                 430         
Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
        435                 440                 445             
Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460             

<210>  5
<211>  389
<212>  PRT
<213>  Artificial Sequence

<400>  5
Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      
Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          
Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              
Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  
Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  
Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      
Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ser Gly Gly Gly Ser 
            100                 105                 110         
Gly Ala Leu Glu Ser Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu 
        115                 120                 125             
Ser Met Glu Pro Cys Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe 
    130                 135                 140                 
Cys Arg Glu Ser Gly Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys 
145                 150                 155                 160 
Glu Lys Leu Arg Arg Arg Phe Ser Ser Leu His Phe Met Val Glu Val 
                165                 170                 175     
Lys Gly Asp Leu Thr Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu 
            180                 185                 190         
Ala Gln Gln Asp His Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu 
        195                 200                 205             
Ser His Gly Cys Gln Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr 
    210                 215                 220                 
Gly Thr Asp Gly Cys Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe 
225                 230                 235                 240 
Asn Gly Thr Ser Cys Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe 
                245                 250                 255     
Ile Gln Ala Cys Gly Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala 
            260                 265                 270         
Ser Thr Ser Pro Glu Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp 
        275                 280                 285             
Ala Thr Pro Phe Gln Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala 
    290                 295                 300                 
Ile Ser Ser Leu Pro Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr 
305                 310                 315                 320 
Phe Pro Gly Phe Val Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr 
                325                 330                 335     
Val Glu Thr Leu Asp Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp 
            340                 345                 350         
Leu Gln Ser Leu Leu Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly 
        355                 360                 365             
Ile Tyr Lys Gln Met Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu 
    370                 375                 380                 
Phe Phe Lys Thr Ser 
385                 

<210>  6
<211>  720
<212>  DNA
<213>  Artificial Sequence

<400>  6
gacatccagc tgacccagtc tcacaaattc ctgtccactt cagtaggaga cagggtcagc        60
atcacctgca aggccagtca ggatgtgtat aatgctgttg cctggtatca acagaaacca       120
ggacaatctc ctaaacttct gatttactcg gcatcctccc ggtacactgg agtcccttct       180
cgcttcactg gcagtggctc tgggccggat ttcactttca ccatcagcag tgtgcaggct       240
gaagacctgg cagtttattt ctgtcagcaa cattttcgta ctccattcac gttcggctcg       300
gggacaaaat tggagatcgg cggtggcggt tctggtggcg gtggctccgg cggtggcggt       360
tctcaggtac aactgcagca gtctggacct gaactgaaga agcctggaga gacagtcaag       420
atctcctgca aggcctctgg gtatcctttc acaaactatg gaatgaactg ggtgaagcag       480
gctccaggac agggtttaaa gtggatgggc tggattaaca cttccactgg agagtcaaca       540
tttgctgatg acttcaaggg acggtttgac ttctctttgg aaacctctgc caacactgcc       600
tatttgcaga tcaacaacct caaaagtgaa gacatggcta catatttctg tgcaagatgg       660
gaggtttacc acggctacgt tccttactgg ggccaaggga ccacggtcac cgtttcctct       720

<210>  7
<211>  1389
<212>  DNA
<213>  Artificial Sequence

<400>  7
gacatccagc tgacccagtc tcacaaattc ctgtccactt cagtaggaga cagggtcagc        60
atcacctgca aggccagtca ggatgtgtat aatgctgttg cctggtatca acagaaacca       120
ggacaatctc ctaaacttct gatttactcg gcatcctccc ggtacactgg agtcccttct       180
cgcttcactg gcagtggctc tgggccggat ttcactttca ccatcagcag tgtgcaggct       240
gaagacctgg cagtttattt ctgtcagcaa cattttcgta ctccattcac gttcggctcg       300
gggacaaaat tggagatcgg cggtggcggt tctggtggcg gtggctccgg cggtggcggt       360
tctcaggtac aactgcagca gtctggacct gaactgaaga agcctggaga gacagtcaag       420
atctcctgca aggcctctgg gtatcctttc acaaactatg gaatgaactg ggtgaagcag       480
gctccaggac agggtttaaa gtggatgggc tggattaaca cttccactgg agagtcaaca       540
tttgctgatg acttcaaggg acggtttgac ttctctttgg aaacctctgc caacactgcc       600
tatttgcaga tcaacaacct caaaagtgaa gacatggcta catatttctg tgcaagatgg       660
gaggtttacc acggctacgt tccttactgg ggccaaggga ccacggtcac cgtttcctct       720
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       780
tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg       840
gacttcgcct gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc       900
ctgtcactgg ttatcaccct ttactgcaaa cggggcagaa agaaactcct gtatatattc       960
aaacaaccat ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga      1020
tttccagaag aagaagaagg aggatgtgaa ctgagagtga agttcagcag gagcgcagac      1080
gcccccgcgt acaagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga      1140
gaggagtacg atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg      1200
agaaggaaga accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag      1260
gcctacagtg agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt      1320
taccagggtc tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg      1380
ccccctcgc                                                              1389

<210>  8
<211>  321
<212>  DNA
<213>  Artificial Sequence

<400>  8
ggagtgcagg tggaaaccat ctccccagga gacgggcgca ccttccccaa gcgcggccag        60
acctgcgtgg tgcactacac cgggatgctt gaagatggaa agaaatttga ttcctcccgg       120
gacagaaaca agccctttaa gtttatgcta ggcaagcagg aggtgatccg aggctgggaa       180
gaaggggttg cccagatgag tgtgggtcag agagccaaac tgactatatc tccagattat       240
gcctatggtg ccactgggca cccaggcatc atcccaccac atgccactct cgtcttcgat       300
gtggagcttc taaaactgga a                                                 321

<210>  9
<211>  831
<212>  DNA
<213>  Artificial Sequence

<400>  9
ggtgctcttg agagtttgag gggaaatgca gatttggctt acatcctgag catggagccc        60
tgtggccact gcctcattat caacaatgtg aacttctgcc gtgagtccgg gctccgcacc       120
cgcactggct ccaacatcga ctgtgagaag ttgcggcgtc gcttctcctc gctgcatttc       180
atggtggagg tgaagggcga cctgactgcc aagaaaatgg tgctggcttt gctggagctg       240
gcgcagcagg accacggtgc tctggactgc tgcgtggtgg tcattctctc tcacggctgt       300
caggccagcc acctgcagtt cccaggggct gtctacggca cagatggatg ccctgtgtcg       360
gtcgagaaga ttgtgaacat cttcaatggg accagctgcc ccagcctggg agggaagccc       420
aagctctttt tcatccaggc ctgtggtggg gagcagaaag accatgggtt tgaggtggcc       480
tccacttccc ctgaagacga gtcccctggc agtaaccccg agccagatgc caccccgttc       540
caggaaggtt tgaggacctt cgaccagctg gacgccatat ctagtttgcc cacacccagt       600
gacatctttg tgtcctactc tactttccca ggttttgttt cctggaggga ccccaagagt       660
ggctcctggt acgttgagac cctggacgac atctttgagc agtgggctca ctctgaagac       720
ctgcagtccc tcctgcttag ggtcgctaat gctgtttcgg tgaaagggat ttataaacag       780
atgcctggtt gctttaattt cctccggaaa aaacttttct ttaaaacatc a                831

<210>  10
<211>  5
<212>  PRT
<213>  Artificial Sequence

<400>  10
Ser Gly Gly Gly Ser 
1               5   

<210>  11
<211>  247
<212>  DNA
<213>  Artificial Sequence

<400>  11
ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag        60
cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg       120
gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac       180
aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa       240
gcgtatt                                                                 247

<210>  12
<211>  60
<212>  DNA
<213>  Artificial Sequence

<400>  12
gccctgcctg tgacagccct gctgctgcct ctggctctgc tgctgcatgc cgctagaccc        60

