                         SEQUENCE LISTING

<110>  PTT Global Chemical Public Company Limited
 
<120>  Yeast having improvement of lactic acid tolerance and use thereof

<130>  BU62-PCT-PI2019

<140>  PCT/TH2019/000030
<141>  2019-08-19

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  6319
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pHAA1del2 Plasmid

<400>  1
gttgacagta agacgggtaa gcctgttgat gataccgctg ccttactggg tgcattagcc       60

agtctgaatg acctgtcacg ggataatccg aagtggtcag actggaaaat cagagggcag      120

gaactgctga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc      180

tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat gaatccataa      240

aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagcg cagcgaactg      300

aatgtcacga aaaagacagc gactcaggtg cctgatggtc ggagacaaaa ggaatattca      360

gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa ggtctgtttt      420

gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact gactaaagta      480

gtgagttata cacagggctg ggatctattc tttttatctt tttttattct ttctttattc      540

tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta gcggaattta      600

cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat ttacagatac      660

ccacaactca aaggaaaagg actagtaatt atcattgact agcccatctc aattggtata      720

gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt ttcaaagcaa      780

atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta attttatgct      840

gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg acggtatcgt      900

tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct tatggtgtat      960

tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat cctttggtta     1020

aaggctttga gattttccag tggacaaact atgccaagtt ctcaagcgaa aaattagaat     1080

tagtttttag tgaagagata ttgccttatc ttttccagtt aaaaaaattc ataaaatata     1140

atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat gagtggttat     1200

taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc cttgatgaat     1260

ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt aaccaatggg     1320

ttttgaaacc aataagtaaa gatttaaaca cttacagcaa tatgaaattg gtggttgata     1380

agcgaggccg cccgactgat acgttgattt tccaagttga actagataga caaatggatc     1440

tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata ccaacaacca     1500

ttacatcaga ttcctaccta cgtaacggac taagaaaaac actacacgat gctttaactg     1560

caaaaattca gctcaccagt tttgaggcaa aatttttgag tgacatgcaa agtaagcatg     1620

atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta gagaacatac     1680

tggctaaata cggaaggatc tgaggttctt atggccatta gccatcatcg tatccgatat     1740

tcaagtatca tcacacttac caacacaatt attttttttt ttttttattt taaatattaa     1800

gaaaagagag aataaagaag aagtggtaaa agacaaacaa ggagcaagcg tttagtgggg     1860

atctaacaaa caacacaaca acaacatact ctgtgctttg aagtgtaaac tactatatca     1920

ttatcattaa tttttggata ttggattttg gttttttttt tctgtcgaga acctctcatt     1980

acttgggctt taatttaaag tcataaatta ttcgttttat ttcatttcat ttcattttat     2040

tttattttaa tattgggaaa tttactctct caatagtgtg tgcttttaaa aaccgggttc     2100

caaaataggt tttagcgcac agatacgggt tctggggaaa caccagcagc agcagtagcg     2160

aaagttttcc accgtctcca gccttcttcc tggtcatttt tttcgataca agccaactta     2220

ataacaaata tatatatgtg ttatctattt ctacctaatt aaatcggtac aatccgttat     2280

tgaatgtaat agttctaatt atatttgtta gcacgtcttt cattttttcc cccaaatgct     2340

ctatatatat tcttactctc gctactatat tttatataat aagagactaa tcgataatat     2400

caaattttaa attatacttg ttgttaccca tctatataca ctgaacccta tccgagagca     2460

cataggtgta tgacaatccg gtatcaaacc attcaatggg agatatactg gaaaacaaaa     2520

cttatatctt tgtagttgtg ctcattcttt agtagatggt cattggtttt tgccaatgca     2580

ctgtgatcca aatggttcca cgaggtagca tcaccataca acattacagg cgcttttatc     2640

atttgtttac catttttgca acgcttacag ttgtatgtct cgttcaattc atcatttttt     2700

ttttattctt ttttttgatt tcggtttctt tgaaattttt ttgattcggt aatctccgaa     2760

cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt     2820

gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc     2880

aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct     2940

agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct     3000

tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa     3060

atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt     3120

aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt     3180

gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa     3240

tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag     3300

caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca     3360

tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc     3420

gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt     3480

tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt     3540

caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga     3600

agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca     3660

ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta     3720

aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc     3780

gggaatctcg gtcgtaatga tttttataat gacgaaaaaa aaaaaattgg aaagaaaaag     3840

cttggacaca agtaaaaaca cagtcgggct tgaaccgcta agtataatga aacccatcac     3900

tccaaatact agaacaaagg ttggtgaagt ctatatccca ctaacagagt acgtcccaac     3960

aagtataacg tcttctcacg accaaacaga cagcatcaac ccacttctca tgggcgacag     4020

cctgtccata tatgacgatg gccagtctga tactcctaat gtaacatact tcgctgatac     4080

ttctaaaagt atgtcgtata cccagttgaa ggaacagcgg aagtctgtca acaatgaaca     4140

cgttccttca caaagagtgc ctctagccaa cactggtcgc tcctcttcct ttatctctaa     4200

tatatcatct catgactccg taatctcgaa taacgatttc ataagttcga tgaacagttc     4260

ggattctctt tcgtcgatgc tgcaaggtgg taacgctcac cattaccagg atatgctcca     4320

tcaccccagc aatggcagag gcatggacat aagcctgttc ggttcgtaag ctgtaatgca     4380

agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg     4440

gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc     4500

gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca     4560

acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg     4620

tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg     4680

aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac     4740

acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag     4800

ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg     4860

ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc     4920

gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag     4980

ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct     5040

tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg     5100

cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa     5160

atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga     5220

aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac     5280

ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc     5340

agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat     5400

gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat     5460

gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt     5520

taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt     5580

cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc tagcgagggc     5640

tttactaagc tgatccggtg gatgaccttt tgaatgacct ttaatagatt atattactaa     5700

ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac aaaacggttt     5760

acaagcatac gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa     5820

gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct     5880

ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac     5940

acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt cgactctaga     6000

ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga     6060

aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg     6120

taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga     6180

atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg     6240

gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc     6300

aacacccgct gacgaattc                                                  6319


<210>  2
<211>  599
<212>  PRT
<213>  Kluyveromyces marxianus

<400>  2

Met Val Leu Ile Asn Gly Val Lys Tyr Ala Cys Glu Arg Cys Ile Arg 
1               5                   10                  15      


Gly His Arg Val Thr Thr Cys Asn His Thr Asp Gln Pro Leu Thr Met 
            20                  25                  30          


Ile Lys Pro Lys Gly Arg Pro Ser Thr Thr Cys Ala His Cys Lys Glu 
        35                  40                  45              


Leu Arg Lys Ser Lys Asn Ala Asn Pro Ser Gly Gln Cys Thr Cys Gly 
    50                  55                  60                  


Arg Gln Asp Lys Lys Arg Leu Ala Gln Lys Val Lys Glu Glu Ala Ser 
65                  70                  75                  80  


Cys Thr Cys Lys Thr Asp Pro Asp His Cys Ala Cys His Lys Lys Arg 
                85                  90                  95      


Gly Ala Lys Arg Lys Thr Lys Gly Gln Asn Ser Gly Thr Ser Ile Gly 
            100                 105                 110         


Leu Asp Leu Asp Gly Lys Val Ser Lys Ser Asn Gly Tyr Ser Phe Gln 
        115                 120                 125             


Ser Leu Pro Ser Ile Asn Ser Ser Gln Ser Leu Asp Lys Asp Ile Ser 
    130                 135                 140                 


Asn Leu Leu Gly Ser Pro Ile Ser Met Asn Thr Ser Phe Ser Thr Gly 
145                 150                 155                 160 


Trp Asp Thr Gly Ser Ile Ser Ser Ser Asn Arg Ser Pro Pro Gly Ser 
                165                 170                 175     


Gly Asn Thr Tyr Ser Asn Ile Ser Asn Gly Asn Thr Gly Thr Ile Ser 
            180                 185                 190         


Asn Asn Asn Asn Asn Asn Asn Asn Asn His His His His His Lys His 
        195                 200                 205             


Gly Gln Asp Thr Ser Lys Asn Thr Val Gly Leu Glu Pro Leu Ser Ile 
    210                 215                 220                 


Met Lys Pro Ile Thr Pro Asn Thr Arg Thr Lys Val Gly Glu Val Tyr 
225                 230                 235                 240 


Ile Pro Leu Thr Glu Tyr Val Pro Thr Ser Ile Thr Ser Ser His Asp 
                245                 250                 255     


Gln Thr Asp Ser Ile Asn Pro Leu Leu Met Gly Asp Ser Leu Ser Ile 
            260                 265                 270         


Tyr Asp Asp Gly Gln Ser Asp Thr Pro Asn Val Thr Tyr Phe Ala Asp 
        275                 280                 285             


Thr Ser Lys Ser Met Ser Tyr Thr Gln Leu Lys Glu Gln Arg Lys Ser 
    290                 295                 300                 


Val Asn Asn Glu His Val Pro Ser Gln Arg Val Pro Leu Ala Asn Thr 
305                 310                 315                 320 


Gly Arg Ser Ser Ser Phe Ile Ser Asn Ile Ser Ser His Asp Ser Val 
                325                 330                 335     


Ile Ser Asn Asn Asp Phe Ile Ser Ser Met Asn Ser Ser Asp Ser Leu 
            340                 345                 350         


Ser Ser Met Leu Gln Gly Gly Asn Ala His His Tyr Gln Asp Met Leu 
        355                 360                 365             


His His Pro Ser Asn Gly Arg Gly Ser Gly Phe Asn Pro Val His Ile 
    370                 375                 380                 


Glu Gln Ser Pro Asn Val Tyr Gly Phe Asp Thr Asp Ser Val Arg Ser 
385                 390                 395                 400 


Val Glu Val Leu Ser Ile Thr Pro Ser Phe Met Asp Ile Pro Glu Ser 
                405                 410                 415     


Lys Gln Ala Ser Ala Glu Ser Asn Thr Ser Ser Ser Gly Tyr Ile Ser 
            420                 425                 430         


Trp Lys Gly Val Asn Ser Arg Arg Glu Arg Ser Val Ser Ile His Lys 
        435                 440                 445             


Asn His Arg Tyr Asp Ser Glu Asn Lys Arg Lys Arg His Pro Leu Thr 
    450                 455                 460                 


Ser Ala Asn Ser Ser Lys Gln Gln Ile Lys Ser Met Ile Leu Pro Ile 
465                 470                 475                 480 


Glu Glu Asn Asn Asn His Asn Ser Ser Ser Ser Asn Glu Asn Thr Ala 
                485                 490                 495     


Ala Thr Leu Pro Thr Thr Ser Asn Asn Phe Asn Ser Pro Ala Asp Ser 
            500                 505                 510         


Thr Asn Thr Ala Leu Ser Ser Lys Ala Ala Phe Gly Asp Gln Ser Val 
        515                 520                 525             


Phe Ser Thr Glu Arg Arg Gly Leu Glu Pro Ser Phe Val Asp Pro Gln 
    530                 535                 540                 


Phe Ser Pro Asp Phe Asn Pro Ser Leu Lys Gln Ser His Met Ile Asn 
545                 550                 555                 560 


Gln Asn Ile Ile Asp Asn Asn Ser Leu Phe Ser Gly Thr Ser Glu Gly 
                565                 570                 575     


Asn Asn Pro Ser Pro Asn Asp Ile Phe Pro Val Glu Phe Ala Asp Ile 
            580                 585                 590         


Asp Asp Leu Met Thr His Leu 
        595                 


