﻿               SEQUENCE LISTING

<110> Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.

<120> Production of MHC II/CII complexes

<130> 115598P1217PC

<150> EP19191094
<151> 2019-08-09

<160> 34

<170> BiSSAP 1.3.6

<210> 1
<211> 11
<212> PRT
<213> Homo sapiens


<220> 
<223> CII peptide 261-271

<400> 1
Ala Gly Phe Lys Gly Glu Gln Gly Pro Lys Gly 
1               5                   10      

<210> 2
<211> 11
<212> PRT
<213> Homo sapiens


<220> 
<223> CII peptide 261-271, Lys = Xaa

<220> 
<221> UNSURE
<222> 10
<223> Xaa = any proteinogenic amino acids except Lys; preferably Xaa =
      Arg, Ala, Gly or Gln

<400> 2
Ala Gly Phe Lys Gly Glu Gln Gly Pro Xaa Gly 
1               5                   10      

<210> 3
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-271; Gln = Xaa

<220> 
<221> UNSURE
<222> 7
<223> Xaa = any proteinogenic amion acid except Gln; preferably Xaa =
      Ala, Arg, His or Gly

<400> 3
Ala Gly Phe Lys Gly Glu Xaa Gly Pro Lys Gly 
1               5                   10      

<210> 4
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-271; Glu = Xaa

<220> 
<221> UNSURE
<222> 6
<223> Xaa = any proteinogenic amino acid except Glu, preferably Xaa =
      Ala, Asp, Gln or Gly

<400> 4
Ala Gly Phe Lys Gly Xaa Gln Gly Pro Lys Gly 
1               5                   10      

<210> 5
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-271; Gly = Xaa

<220> 
<221> UNSURE
<222> 5
<223> Xaa = any proteinogenic amino acid expect Gly; preferably Xaa =
      Ala, Ser, Val or Leu

<400> 5
Ala Gly Phe Lys Xaa Glu Gln Gly Pro Lys Gly 
1               5                   10      

<210> 6
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-271; Gln = Xaa and Lys = Xaa

<220> 
<221> UNSURE
<222> 7
<223> Xaa = any proteinogenic amino acid expect Gln; preferably Xaa =
      Ala, Arg, His or Gly

<220> 
<221> UNSURE
<222> 10
<223> Xaa = any proteinogenic amino acid except Lys; preferably Xaa =
      Arg, Ala, Gly or Gln

<400> 6
Ala Gly Phe Lys Gly Glu Xaa Gly Pro Xaa Gly 
1               5                   10      

<210> 7
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-271; Glu = Xaa and Lys = Xaa

<220> 
<221> UNSURE
<222> 6
<223> Xaa = any proteinogenic amino acid except Glu; preferably Xaa =
      Ala, Asp, Gln or Gly

<220> 
<221> UNSURE
<222> 10
<223> Xaa = any proteinogenic amino acid expect Lys, preferably Xaa =
      Arg, Ala, Gly or Gln

<400> 7
Ala Gly Phe Lys Gly Xaa Gln Gly Pro Xaa Gly 
1               5                   10      

<210> 8
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-271; Gly = Xaa and Lys = Xaa

<220> 
<221> UNSURE
<222> 5
<223> Xaa = any proteinogenic amino acid except Gly; preferably Xaa =
      Ala, Ser, Val or Leu

<400> 8
Ala Gly Phe Lys Xaa Glu Gln Gly Pro Xaa Gly 
1               5                   10      

<210> 9
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-271; Lys = Arg

<400> 9
Ala Gly Phe Lys Gly Glu Gln Gly Pro Arg Gly 
1               5                   10      

<210> 10
<211> 13
<212> PRT
<213> Homo sapiens


<220> 
<223> CII peptide 261-273

<400> 10
Ala Gly Phe Lys Gly Glu Gln Gly Pro Lys Gly Glu Pro 
1               5                   10              

<210> 11
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-273; Lys = Xaa

<220> 
<221> UNSURE
<222> 10
<223> Xaa = any proteinogenic amino acid except Lys; preferably Lys =
      Arg, Ala, Gly or Gln

<400> 11
Ala Gly Phe Lys Gly Glu Gln Gly Pro Xaa Gly Glu Pro 
1               5                   10              

<210> 12
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 261-273; Lys = Arg

<400> 12
Ala Gly Phe Lys Gly Glu Gln Gly Pro Arg Gly Glu Pro 
1               5                   10              

<210> 13
<211> 15
<212> PRT
<213> Homo sapiens


<220> 
<223> CII peptide 259-273

<400> 13
Gly Ile Ala Gly Phe Lys Gly Glu Gln Gly Pro Lys Gly Glu Pro 
1               5                   10                  15  

<210> 14
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 259-273; Lys = Xaa

<220> 
<221> UNSURE
<222> 12
<223> Xaa = any proteinogenic amino acid; preferably Xaa = Arg, Ala,
      Gly or Gln

<400> 14
Gly Ile Ala Gly Phe Lys Gly Glu Gln Gly Pro Xaa Gly Glu Pro 
1               5                   10                  15  

<210> 15
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> CII peptide 259-273; Lys = Arg

<400> 15
Gly Ile Ala Gly Phe Lys Gly Glu Gln Gly Pro Arg Gly Glu Pro 
1               5                   10                  15  

<210> 16
<211> 275
<212> PRT
<213> Artificial Sequence


<220> 
<223> DR4 construct alpha-chain

<400> 16
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ile Lys Glu Glu His Val Ile Ile Gln Ala Glu Phe Tyr Leu 
            20                  25                  30          
Asn Pro Asp Gln Ser Gly Glu Phe Met Phe Asp Phe Asp Gly Asp Glu 
        35                  40                  45              
Ile Phe His Val Asp Met Ala Lys Lys Glu Thr Val Trp Arg Leu Glu 
    50                  55                  60                  
Glu Phe Gly Arg Phe Ala Ser Phe Glu Ala Gln Gly Ala Leu Ala Asn 
65                  70                  75                  80  
Ile Ala Val Asp Lys Ala Asn Leu Glu Ile Met Thr Lys Arg Ser Asn 
                85                  90                  95      
Tyr Thr Pro Ile Thr Asn Val Pro Pro Glu Val Thr Val Leu Thr Asn 
            100                 105                 110         
Ser Pro Val Glu Leu Arg Glu Pro Asn Val Leu Ile Cys Phe Ile Asp 
        115                 120                 125             
Lys Phe Thr Pro Pro Val Val Asn Val Thr Trp Leu Arg Asn Gly Lys 
    130                 135                 140                 
Pro Val Thr Thr Gly Val Ser Glu Thr Val Phe Leu Pro Arg Glu Asp 
145                 150                 155                 160 
His Leu Phe Arg Lys Phe His Tyr Leu Pro Phe Leu Pro Ser Thr Glu 
                165                 170                 175     
Asp Val Tyr Asp Cys Arg Val Glu His Trp Gly Leu Asp Glu Pro Leu 
            180                 185                 190         
Leu Lys His Trp Glu Phe Asp Ala Ser Gly Gly Gly Glu Asn Leu Tyr 
        195                 200                 205             
Phe Gln Gly Gly Gly Gly Ser Leu Thr Asp Thr Leu Gln Ala Glu Thr 
    210                 215                 220                 
Asp Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn 
225                 230                 235                 240 
Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe Ile Leu Ala Ala His Gly 
                245                 250                 255     
Gly Gly Gly Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu 
            260                 265                 270         
Trp His Glu 
        275 

<210> 17
<211> 311
<212> PRT
<213> Artificial Sequence


<220> 
<223> DR4 construct beta-chain with hCII259-273 peptide

<400> 17
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Ile Ala Gly 
            20                  25                  30          
Phe Lys Gly Glu Gln Gly Pro Lys Gly Glu Pro Ser Gly Gly Gly Ser 
        35                  40                  45              
Leu Val Pro Arg Gly Ser Gly Gly Gly Gly Ser Gly Asp Thr Arg Pro 
    50                  55                  60                  
Arg Phe Leu Glu Gln Val Lys His Glu Cys His Phe Phe Asn Gly Thr 
65                  70                  75                  80  
Glu Arg Val Arg Phe Leu Asp Arg Tyr Phe Tyr His Gln Glu Glu Tyr 
                85                  90                  95      
Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr Glu Leu 
            100                 105                 110         
Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu Leu Glu 
        115                 120                 125             
Gln Lys Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr Gly Val 
    130                 135                 140                 
Gly Glu Ser Phe Thr Val Gln Arg Arg Val Tyr Pro Glu Val Thr Val 
145                 150                 155                 160 
Tyr Pro Ala Lys Thr Gln Pro Leu Gln His His Asn Leu Leu Val Cys 
                165                 170                 175     
Ser Val Asn Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp Phe Arg 
            180                 185                 190         
Asn Gly Gln Glu Glu Lys Thr Gly Val Val Ser Thr Gly Leu Ile Gln 
        195                 200                 205             
Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr Val Pro 
    210                 215                 220                 
Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser Leu Thr 
225                 230                 235                 240 
Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Gly Gly Gly Glu Asn 
                245                 250                 255     
Leu Tyr Phe Gln Gly Gly Gly Gly Ser Arg Ile Ala Arg Leu Glu Glu 
            260                 265                 270         
Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala 
        275                 280                 285             
Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Val Met Asn 
    290                 295                 300                 
His His His His His His His 
305                 310     

<210> 18
<211> 248
<212> PRT
<213> Artificial Sequence


<220> 
<223> Minimal DR4 construct alpha-chain

<400> 18
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ile Lys Glu Glu His Val Ile Ile Gln Ala Glu Phe Tyr Leu 
            20                  25                  30          
Asn Pro Asp Gln Ser Gly Glu Phe Met Phe Asp Phe Asp Gly Asp Glu 
        35                  40                  45              
Ile Phe His Val Asp Met Ala Lys Lys Glu Thr Val Trp Arg Leu Glu 
    50                  55                  60                  
Glu Phe Gly Arg Phe Ala Ser Phe Glu Ala Gln Gly Ala Leu Ala Asn 
65                  70                  75                  80  
Ile Ala Val Asp Lys Ala Asn Leu Glu Ile Met Thr Lys Arg Ser Asn 
                85                  90                  95      
Tyr Thr Pro Ile Thr Asn Val Pro Pro Glu Val Thr Val Leu Thr Asn 
            100                 105                 110         
Ser Pro Val Glu Leu Arg Glu Pro Asn Val Leu Ile Cys Phe Ile Asp 
        115                 120                 125             
Lys Phe Thr Pro Pro Val Val Asn Val Thr Trp Leu Arg Asn Gly Lys 
    130                 135                 140                 
Pro Val Thr Thr Gly Val Ser Glu Thr Val Phe Leu Pro Arg Glu Asp 
145                 150                 155                 160 
His Leu Phe Arg Lys Phe His Tyr Leu Pro Phe Leu Pro Ser Thr Glu 
                165                 170                 175     
Asp Val Tyr Asp Cys Arg Val Glu His Trp Gly Leu Asp Glu Pro Leu 
            180                 185                 190         
Leu Lys His Trp Glu Phe Asp Ala Ser Gly Gly Gly Gly Gly Gly Ser 
        195                 200                 205             
Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu Asp Glu Lys 
    210                 215                 220                 
Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys 
225                 230                 235                 240 
Leu Glu Phe Ile Leu Ala Ala His 
                245             

<210> 19
<211> 288
<212> PRT
<213> Artificial Sequence


<220> 
<223> Minimal DR4 construct beta-chain with hCII259-273 peptide

<400> 19
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Gly Ile Ala Gly Phe Lys Gly Glu Gln Gly Pro Lys Gly Glu 
            20                  25                  30          
Pro Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Asp Thr Arg Pro 
        35                  40                  45              
Arg Phe Leu Glu Gln Val Lys His Glu Cys His Phe Phe Asn Gly Thr 
    50                  55                  60                  
Glu Arg Val Arg Phe Leu Asp Arg Tyr Phe Tyr His Gln Glu Glu Tyr 
65                  70                  75                  80  
Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr Glu Leu 
                85                  90                  95      
Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu Leu Glu 
            100                 105                 110         
Gln Lys Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr Gly Val 
        115                 120                 125             
Gly Glu Ser Phe Thr Val Gln Arg Arg Val Tyr Pro Glu Val Thr Val 
    130                 135                 140                 
Tyr Pro Ala Lys Thr Gln Pro Leu Gln His His Asn Leu Leu Val Cys 
145                 150                 155                 160 
Ser Val Asn Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp Phe Arg 
                165                 170                 175     
Asn Gly Gln Glu Glu Lys Thr Gly Val Val Ser Thr Gly Leu Ile Gln 
            180                 185                 190         
Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr Val Pro 
        195                 200                 205             
Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser Leu Thr 
    210                 215                 220                 
Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Gly Gly Gly Gly Gly 
225                 230                 235                 240 
Gly Ser Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala 
                245                 250                 255     
Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val 
            260                 265                 270         
Ala Gln Leu Lys Gln Lys Val Met Asn His His His His His His His 
        275                 280                 285             



<210> 20
<211> 311
<212> PRT
<213> Artificial Sequence


<220> 
<223> DR4 construct beta-chain with hCLIPmut

<400> 20
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Pro Val Ser Lys 
            20                  25                  30          
Ala Arg Met Ala Thr Gly Ala Leu Ala Gln Ala Ser Gly Gly Gly Ser 
        35                  40                  45              
Leu Val Pro Arg Gly Ser Gly Gly Gly Gly Ser Gly Asp Thr Arg Pro 
    50                  55                  60                  
Arg Phe Leu Glu Gln Val Lys His Glu Cys His Phe Phe Asn Gly Thr 
65                  70                  75                  80  
Glu Arg Val Arg Phe Leu Asp Arg Tyr Phe Tyr His Gln Glu Glu Tyr 
                85                  90                  95      
Val Arg Phe Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr Glu Leu 
            100                 105                 110         
Gly Arg Pro Asp Ala Glu Tyr Trp Asn Ser Gln Lys Asp Leu Leu Glu 
        115                 120                 125             
Gln Lys Arg Ala Ala Val Asp Thr Tyr Cys Arg His Asn Tyr Gly Val 
    130                 135                 140                 
Gly Glu Ser Phe Thr Val Gln Arg Arg Val Tyr Pro Glu Val Thr Val 
145                 150                 155                 160 
Tyr Pro Ala Lys Thr Gln Pro Leu Gln His His Asn Leu Leu Val Cys 
                165                 170                 175     
Ser Val Asn Gly Phe Tyr Pro Gly Ser Ile Glu Val Arg Trp Phe Arg 
            180                 185                 190         
Asn Gly Gln Glu Glu Lys Thr Gly Val Val Ser Thr Gly Leu Ile Gln 
        195                 200                 205             
Asn Gly Asp Trp Thr Phe Gln Thr Leu Val Met Leu Glu Thr Val Pro 
    210                 215                 220                 
Arg Ser Gly Glu Val Tyr Thr Cys Gln Val Glu His Pro Ser Leu Thr 
225                 230                 235                 240 
Ser Pro Leu Thr Val Glu Trp Arg Ala Arg Ser Gly Gly Gly Glu Asn 
                245                 250                 255     
Leu Tyr Phe Gln Gly Gly Gly Gly Ser Arg Ile Ala Arg Leu Glu Glu 
            260                 265                 270         
Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala 
        275                 280                 285             
Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Val Met Asn 
    290                 295                 300                 
His His His His His His His 
305                 310     

<210> 21
<211> 290
<212> PRT
<213> Artificial Sequence


<220> 
<223> Aq construct alpha-chain

<400> 21
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Glu Asp Asp Ile Glu Ala Asp His Val Gly Phe Tyr Gly Ile 
            20                  25                  30          
Val Val Tyr Gln Ser Pro Gly Asp Ile Gly Gln Tyr Thr His Glu Phe 
        35                  40                  45              
Asp Gly Asp Glu Trp Phe Tyr Val Asp Leu Asp Lys Lys Glu Thr Val 
    50                  55                  60                  
Trp Met Leu Pro Glu Phe Gly Gln Leu Thr Ser Phe Asp Pro Gln Gly 
65                  70                  75                  80  
Gly Leu Gln Asn Ile Ala Thr Gly Lys His Asn Leu Gly Gly Trp Thr 
                85                  90                  95      
Lys Arg Ser Asn Phe Thr Pro Ala Thr Asn Glu Ala Pro Gln Ala Thr 
            100                 105                 110         
Val Phe Pro Lys Ser Pro Val Leu Leu Gly Gln Pro Asn Thr Leu Ile 
        115                 120                 125             
Cys Phe Val Asp Asn Ile Phe Pro Pro Val Ile Asn Ile Thr Trp Leu 
    130                 135                 140                 
Arg Asn Ser Lys Ser Val Thr Asp Gly Val Tyr Glu Thr Ser Phe Leu 
145                 150                 155                 160 
Val Asn Arg Asp His Ser Phe His Lys Leu Ser Tyr Leu Thr Phe Ile 
                165                 170                 175     
Pro Ser Asp Asp Asp Ile Tyr Asp Cys Lys Val Glu His Trp Gly Leu 
            180                 185                 190         
Asp Glu Pro Val Leu Lys His Trp Glu Pro Glu Ile Pro Ala Thr Met 
        195                 200                 205             
Ser Glu Leu Thr Glu Thr Val Ser Gly Gly Gly Glu Asn Leu Tyr Phe 
    210                 215                 220                 
Gln Gly Gly Gly Gly Ser Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp 
225                 230                 235                 240 
Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu 
                245                 250                 255     
Leu Lys Glu Lys Glu Lys Leu Glu Phe Ile Leu Ala Ala His Gly Gly 
            260                 265                 270         
Gly Gly Ser Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp 
        275                 280                 285             
His Glu 
    290 

<210> 22
<211> 316
<212> PRT
<213> Artificial Sequence


<220> 
<223> Aq construct beta-chain with rat CII259-273 peptide

<400> 22
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Ile Ala Gly 
            20                  25                  30          
Phe Lys Gly Glu Gln Gly Pro Lys Gly Glu Thr Ser Gly Gly Gly Ser 
        35                  40                  45              
Leu Val Pro Arg Gly Ser Gly Gly Gly Gly Ser Glu Arg His Phe Val 
    50                  55                  60                  
Ala Gln Leu Lys Gly Glu Cys Tyr Phe Thr Asn Gly Thr Gln Arg Ile 
65                  70                  75                  80  
Arg Ser Val Asn Arg Tyr Ile Tyr Asn Arg Glu Glu Trp Val Arg Phe 
                85                  90                  95      
Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr Glu Leu Gly Arg Pro 
            100                 105                 110         
Asp Ala Glu Tyr Trp Asn Ser Gln Pro Glu Ile Leu Glu Arg Thr Arg 
        115                 120                 125             
Ala Glu Val Asp Thr Val Cys Arg His Asn Tyr Glu Gly Val Glu Thr 
    130                 135                 140                 
His Thr Ser Leu Arg Arg Leu Glu Gln Pro Asn Val Ala Ile Ser Leu 
145                 150                 155                 160 
Ser Arg Thr Glu Ala Leu Asn His His Asn Thr Leu Val Cys Ser Val 
                165                 170                 175     
Thr Asp Phe Tyr Pro Ala Lys Ile Lys Val Arg Trp Phe Arg Asn Gly 
            180                 185                 190         
Gln Glu Glu Thr Val Gly Val Ser Ser Thr Gln Leu Ile Arg Asn Gly 
        195                 200                 205             
Asp Trp Thr Phe Gln Val Leu Val Met Leu Glu Met Thr Pro His Gln 
    210                 215                 220                 
Gly Glu Val Tyr Thr Cys His Val Glu His Pro Ser Leu Lys Ser Pro 
225                 230                 235                 240 
Ile Thr Val Glu Trp Arg Ala Gln Ser Glu Ser Ala Arg Ser Lys Ser 
                245                 250                 255     
Gly Gly Gly Glu Asn Leu Tyr Phe Gln Gly Gly Gly Gly Ser Arg Ile 
            260                 265                 270         
Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu 
        275                 280                 285             
Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys 
    290                 295                 300                 
Gln Lys Val Met Asn His His His His His His His 
305                 310                 315     

<210> 23
<211> 310
<212> PRT
<213> Artificial Sequence


<220> 
<223> Aq construct beta-chain with rat CII259-273 peptide without
      His-tag

<400> 23
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Ile Ala Gly 
            20                  25                  30          
Phe Lys Gly Glu Gln Gly Pro Lys Gly Glu Thr Ser Gly Gly Gly Ser 
        35                  40                  45              
Leu Val Pro Arg Gly Ser Gly Gly Gly Gly Ser Glu Arg His Phe Val 
    50                  55                  60                  
Ala Gln Leu Lys Gly Glu Cys Tyr Phe Thr Asn Gly Thr Gln Arg Ile 
65                  70                  75                  80  
Arg Ser Val Asn Arg Tyr Ile Tyr Asn Arg Glu Glu Trp Val Arg Phe 
                85                  90                  95      
Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr Glu Leu Gly Arg Pro 
            100                 105                 110         
Asp Ala Glu Tyr Trp Asn Ser Gln Pro Glu Ile Leu Glu Arg Thr Arg 
        115                 120                 125             
Ala Glu Val Asp Thr Val Cys Arg His Asn Tyr Glu Gly Val Glu Thr 
    130                 135                 140                 
His Thr Ser Leu Arg Arg Leu Glu Gln Pro Asn Val Ala Ile Ser Leu 
145                 150                 155                 160 
Ser Arg Thr Glu Ala Leu Asn His His Asn Thr Leu Val Cys Ser Val 
                165                 170                 175     
Thr Asp Phe Tyr Pro Ala Lys Ile Lys Val Arg Trp Phe Arg Asn Gly 
            180                 185                 190         
Gln Glu Glu Thr Val Gly Val Ser Ser Thr Gln Leu Ile Arg Asn Gly 
        195                 200                 205             
Asp Trp Thr Phe Gln Val Leu Val Met Leu Glu Met Thr Pro His Gln 
    210                 215                 220                 
Gly Glu Val Tyr Thr Cys His Val Glu His Pro Ser Leu Lys Ser Pro 
225                 230                 235                 240 
Ile Thr Val Glu Trp Arg Ala Gln Ser Glu Ser Ala Arg Ser Lys Ser 
                245                 250                 255     
Gly Gly Gly Glu Asn Leu Tyr Phe Gln Gly Gly Gly Gly Ser Arg Ile 
            260                 265                 270         
Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu 
        275                 280                 285             
Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys 
    290                 295                 300                 
Gln Lys Val Met Asn His 
305                 310 

<210> 24
<211> 316
<212> PRT
<213> Artificial Sequence


<220> 
<223> Aq construct beta-chain with mCLIP peptide

<400> 24
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Pro Val Ser Gln 
            20                  25                  30          
Ala Arg Met Ala Thr Pro Leu Leu Met Arg Pro Ser Gly Gly Gly Ser 
        35                  40                  45              
Leu Val Pro Arg Gly Ser Gly Gly Gly Gly Ser Glu Arg His Phe Val 
    50                  55                  60                  
Ala Gln Leu Lys Gly Glu Cys Tyr Phe Thr Asn Gly Thr Gln Arg Ile 
65                  70                  75                  80  
Arg Ser Val Asn Arg Tyr Ile Tyr Asn Arg Glu Glu Trp Val Arg Phe 
                85                  90                  95      
Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr Glu Leu Gly Arg Pro 
            100                 105                 110         
Asp Ala Glu Tyr Trp Asn Ser Gln Pro Glu Ile Leu Glu Arg Thr Arg 
        115                 120                 125             
Ala Glu Val Asp Thr Val Cys Arg His Asn Tyr Glu Gly Val Glu Thr 
    130                 135                 140                 
His Thr Ser Leu Arg Arg Leu Glu Gln Pro Asn Val Ala Ile Ser Leu 
145                 150                 155                 160 
Ser Arg Thr Glu Ala Leu Asn His His Asn Thr Leu Val Cys Ser Val 
                165                 170                 175     
Thr Asp Phe Tyr Pro Ala Lys Ile Lys Val Arg Trp Phe Arg Asn Gly 
            180                 185                 190         
Gln Glu Glu Thr Val Gly Val Ser Ser Thr Gln Leu Ile Arg Asn Gly 
        195                 200                 205             
Asp Trp Thr Phe Gln Val Leu Val Met Leu Glu Met Thr Pro His Gln 
    210                 215                 220                 
Gly Glu Val Tyr Thr Cys His Val Glu His Pro Ser Leu Lys Ser Pro 
225                 230                 235                 240 
Ile Thr Val Glu Trp Arg Ala Gln Ser Glu Ser Ala Arg Ser Lys Ser 
                245                 250                 255     
Gly Gly Gly Glu Asn Leu Tyr Phe Gln Gly Gly Gly Gly Ser Arg Ile 
            260                 265                 270         
Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu 
        275                 280                 285             
Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys 
    290                 295                 300                 
Gln Lys Val Met Asn His His His His His His His 
305                 310                 315     

<210> 25
<211> 310
<212> PRT
<213> Artificial Sequence


<220> 
<223> Aq construct beta-chain with mCLIP peptide without His-tag

<400> 25
Met Lys Leu Cys Ile Leu Leu Ala Val Val Ala Phe Val Gly Leu Ser 
1               5                   10                  15      
Leu Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys Pro Val Ser Gln 
            20                  25                  30          
Ala Arg Met Ala Thr Pro Leu Leu Met Arg Pro Ser Gly Gly Gly Ser 
        35                  40                  45              
Leu Val Pro Arg Gly Ser Gly Gly Gly Gly Ser Glu Arg His Phe Val 
    50                  55                  60                  
Ala Gln Leu Lys Gly Glu Cys Tyr Phe Thr Asn Gly Thr Gln Arg Ile 
65                  70                  75                  80  
Arg Ser Val Asn Arg Tyr Ile Tyr Asn Arg Glu Glu Trp Val Arg Phe 
                85                  90                  95      
Asp Ser Asp Val Gly Glu Tyr Arg Ala Val Thr Glu Leu Gly Arg Pro 
            100                 105                 110         
Asp Ala Glu Tyr Trp Asn Ser Gln Pro Glu Ile Leu Glu Arg Thr Arg 
        115                 120                 125             
Ala Glu Val Asp Thr Val Cys Arg His Asn Tyr Glu Gly Val Glu Thr 
    130                 135                 140                 
His Thr Ser Leu Arg Arg Leu Glu Gln Pro Asn Val Ala Ile Ser Leu 
145                 150                 155                 160 
Ser Arg Thr Glu Ala Leu Asn His His Asn Thr Leu Val Cys Ser Val 
                165                 170                 175     
Thr Asp Phe Tyr Pro Ala Lys Ile Lys Val Arg Trp Phe Arg Asn Gly 
            180                 185                 190         
Gln Glu Glu Thr Val Gly Val Ser Ser Thr Gln Leu Ile Arg Asn Gly 
        195                 200                 205             
Asp Trp Thr Phe Gln Val Leu Val Met Leu Glu Met Thr Pro His Gln 
    210                 215                 220                 
Gly Glu Val Tyr Thr Cys His Val Glu His Pro Ser Leu Lys Ser Pro 
225                 230                 235                 240 
Ile Thr Val Glu Trp Arg Ala Gln Ser Glu Ser Ala Arg Ser Lys Ser 
                245                 250                 255     
Gly Gly Gly Glu Asn Leu Tyr Phe Gln Gly Gly Gly Gly Ser Arg Ile 
            260                 265                 270         
Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn Ser Glu 
        275                 280                 285             
Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln Leu Lys 
    290                 295                 300                 
Gln Lys Val Met Asn His 
305                 310 

<210> 26
<211> 40
<212> PRT
<213> Homo sapiens


<220> 
<223> cFos domain

<400> 26
Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu Asp Glu Lys 
1               5                   10                  15      
Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys 
            20                  25                  30          
Leu Glu Phe Ile Leu Ala Ala His 
        35                  40  

<210> 27
<211> 40
<212> PRT
<213> Homo sapiens


<220> 
<223> cJun domain

<400> 27
Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn 
1               5                   10                  15      
Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln 
            20                  25                  30          
Leu Lys Gln Lys Val Met Asn His 
        35                  40  

<210> 28
<211> 15
<212> PRT
<213> Homo sapiens


<220> 
<223> mutated human CLIP-peptide

<400> 28
Pro Val Ser Lys Ala Arg Met Ala Thr Gly Ala Leu Ala Gln Ala 
1               5                   10                  15  

<210> 29
<211> 15
<212> PRT
<213> Homo sapiens


<220> 
<223> rat CII-peptide 259-273

<400> 29
Gly Ile Ala Gly Phe Lys Gly Glu Gln Gly Pro Lys Gly Glu Thr 
1               5                   10                  15  

<210> 30
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> streptavidin-tag

<400> 30
Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
1               5                   10  

<210> 31
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> Chondroitin-binding peptide

<400> 31
Glu Lys Arg Ile Trp Phe Pro Tyr Arg Arg Phe 
1               5                   10      

<210> 32
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> chondroitin-binding peptide

<400> 32
Tyr Lys Thr Asn Phe Arg Arg Tyr Tyr Arg Phe 
1               5                   10      

<210> 33
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> chondroitin-binding peptide

<400> 33
Val Leu Ile Arg His Phe Arg Lys Arg Tyr Tyr 
1               5                   10      

<210> 34
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> hCII259-273 with N-terminal and C-terminal sequence

<400> 34
Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Ile Ala Gly Phe Lys 
1               5                   10                  15      
Gly Glu Gln Gly Pro Lys Gly Glu Pro Ser Gly Gly Gly Ser 
            20                  25                  30  

