                         SEQUENCE LISTING

<110>  Sunshine Lake Pharma Co., Ltd.
 
<120>  CHIMERIC ANTIGEN RECEPTORS AND USES THEREOF

<130>  PP0918WO

<160>  12    

<170>  PatentIn version 3.3

<210>  1
<211>  104
<212>  PRT
<213>  Artificial

<220>
<223>  the amino acid sequence of the immune co-stimulator transmembrane domain  
connected with the immune co-stimulator intracellular segment of CAR3

<400>  1

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys Cys Trp Leu Thr Lys Lys Lys Tyr 
            20                  25                  30          


Ser Ser Ser Val His Asp Pro Asn Gly Glu Tyr Met Phe Met Arg Ala 
        35                  40                  45              


Val Asn Thr Ala Lys Lys Ser Arg Leu Thr Asp Val Thr Leu Lys Arg 
    50                  55                  60                  


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
65                  70                  75                  80  


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
                85                  90                  95      


Glu Glu Glu Gly Gly Cys Glu Leu 
            100                 


<210>  2
<211>  104
<212>  PRT
<213>  Artificial

<220>
<223>  the amino acid sequence of the immune co-stimulator transmembrane domain  
connected with the immune co-stimulator intracellular segment of CAR1

<400>  2

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu 
            20                  25                  30          


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
        35                  40                  45              


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
    50                  55                  60                  


Glu Leu Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp 
65                  70                  75                  80  


Pro Asn Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys 
                85                  90                  95      


Ser Arg Leu Thr Asp Val Thr Leu 
            100                 


<210>  3
<211>  103
<212>  PRT
<213>  Artificial

<220>
<223>  the amino acid sequence of the immune co-stimulator transmembrane domain  
connected with the immune co-stimulator intracellular segment of CAR6

<400>  3

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys Arg Arg Asp Gln Arg Leu Pro Pro 
            20                  25                  30          


Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln 
        35                  40                  45              


Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Lys Arg Gly 
    50                  55                  60                  


Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val 
65                  70                  75                  80  


Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu 
                85                  90                  95      


Glu Glu Gly Gly Cys Glu Leu 
            100             


<210>  4
<211>  104
<212>  PRT
<213>  Artificial

<220>
<223>  the amino acid sequence of the immune co-stimulator transmembrane domain  
connected with the immune co-stimulator intracellular segment of CAR4

<400>  4

Phe Trp Leu Pro Ile Gly Cys Ala Ala Phe Val Val Val Cys Ile Leu 
1               5                   10                  15      


Gly Cys Ile Leu Ile Cys Trp Leu Lys Arg Gly Arg Lys Lys Leu Leu 
            20                  25                  30          


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
        35                  40                  45              


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
    50                  55                  60                  


Glu Leu Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp 
65                  70                  75                  80  


Pro Asn Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys 
                85                  90                  95      


Ser Arg Leu Thr Asp Val Thr Leu 
            100                 


<210>  5
<211>  105
<212>  PRT
<213>  Artificial

<220>
<223>  the amino acid sequence of the immune co-stimulator transmembrane domain  
connected with the immune co-stimulator intracellular segment of CAR7

<400>  5

Val Ala Ala Ile Leu Gly Leu Gly Leu Val Leu Gly Leu Leu Gly Pro 
1               5                   10                  15      


Leu Ala Ile Leu Leu Ala Leu Tyr Leu Leu Arg Arg Asp Gln Arg Leu 
            20                  25                  30          


Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro 
        35                  40                  45              


Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Lys 
    50                  55                  60                  


Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
65                  70                  75                  80  


Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro 
                85                  90                  95      


Glu Glu Glu Glu Gly Gly Cys Glu Leu 
            100                 105 


<210>  6
<211>  105
<212>  PRT
<213>  Artificial

<220>
<223>  the amino acid sequence of the immune co-stimulator transmembrane domain  
connected with the immune co-stimulator intracellular segment of CAR8

<400>  6

Val Ala Ala Ile Leu Gly Leu Gly Leu Val Leu Gly Leu Leu Gly Pro 
1               5                   10                  15      


Leu Ala Ile Leu Leu Ala Leu Tyr Leu Leu Lys Arg Gly Arg Lys Lys 
            20                  25                  30          


Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr 
        35                  40                  45              


Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly 
    50                  55                  60                  


Gly Cys Glu Leu Arg Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys 
65                  70                  75                  80  


Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala 
                85                  90                  95      


Asp Ala His Ser Thr Leu Ala Lys Ile 
            100                 105 


<210>  7
<211>  312
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the nucleic acid encoding the immune co-stimulator transmembrane domain
       and the immune co-stimulator intracellular segment of CAR3

<400>  7
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc     60

accctttact gctgttggct tacaaaaaag aagtattcat ccagtgtgca cgaccctaac    120

ggtgaataca tgttcatgag agcagtgaac acagccaaaa aatctagact cacagatgtg    180

accctaaagc gcggccgcaa gaagctgctg tacatcttca agcagccctt catgcgcccc    240

gtgcagacca cccaggagga ggacggctgt agctgccgct tccccgagga ggaggagggc    300

ggctgcgagc tg                                                        312


<210>  8
<211>  312
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the nucleic acid encoding the immune co-stimulator transmembrane domain
       and the immune co-stimulator intracellular segment of CAR1

<400>  8
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc     60

accctttact gcaagcgcgg ccgcaagaag ctgctgtaca tcttcaagca gcccttcatg    120

cgccccgtgc agaccaccca ggaggaggac ggctgtagct gccgcttccc cgaggaggag    180

gagggcggct gcgagctgtg ttggcttaca aaaaagaagt attcatccag tgtgcacgac    240

cctaacggtg aatacatgtt catgagagca gtgaacacag ccaaaaaatc tagactcaca    300

gatgtgaccc ta                                                        312


<210>  9
<211>  309
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the nucleic acid encoding the immune co-stimulator transmembrane domain
       and the immune co-stimulator intracellular segment of CAR6

<400>  9
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc     60

accctttact gccggcgcga ccagcgcctg ccccccgacg cccacaagcc ccccggcggc    120

ggcagcttcc gcacccccat ccaggaggag caggccgacg cccacagcac cctggccaag    180

atcaagcgcg gccgcaagaa gctgctgtac atcttcaagc agcccttcat gcgccccgtg    240

cagaccaccc aggaggagga cggctgtagc tgccgcttcc ccgaggagga ggagggcggc    300

tgcgagctg                                                            309


<210>  10
<211>  312
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the nucleic acid encoding the immune co-stimulator transmembrane domain
       and the immune co-stimulator intracellular segment of CAR4

<400>  10
ttctggttac ccataggatg tgcagccttt gttgtagtct gcattttggg atgcatactt     60

atttgttggc ttaagcgcgg ccgcaagaag ctgctgtaca tcttcaagca gcccttcatg    120

cgccccgtgc agaccaccca ggaggaggac ggctgtagct gccgcttccc cgaggaggag    180

gagggcggct gcgagctgtg ttggcttaca aaaaagaagt attcatccag tgtgcacgac    240

cctaacggtg aatacatgtt catgagagca gtgaacacag ccaaaaaatc tagactcaca    300

gatgtgaccc ta                                                        312


<210>  11
<211>  315
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the nucleic acid encoding the immune co-stimulator transmembrane domain
       and the immune co-stimulator intracellular segment of CAR7

<400>  11
gttgccgcca tcctgggcct gggcctggtg ctggggctgc tgggccccct ggccatcctg     60

ctggccctgt acctgctccg gcgcgaccag cgcctgcccc ccgacgccca caagcccccc    120

ggcggcggca gcttccgcac ccccatccag gaggagcagg ccgacgccca cagcaccctg    180

gccaagatca agcgcggccg caagaagctg ctgtacatct tcaagcagcc cttcatgcgc    240

cccgtgcaga ccacccagga ggaggacggc tgtagctgcc gcttccccga ggaggaggag    300

ggcggctgcg agctg                                                     315


<210>  12
<211>  315
<212>  DNA
<213>  Artificial

<220>
<223>  the nucleotide sequence of the nucleic acid encoding the immune co-stimulator transmembrane domain
       and the immune co-stimulator intracellular segment of CAR8

<400>  12
gttgccgcca tcctgggcct gggcctggtg ctggggctgc tgggccccct ggccatcctg     60

ctggccctgt acctgctcaa gcgcggccgc aagaagctgc tgtacatctt caagcagccc    120

ttcatgcgcc ccgtgcagac cacccaggag gaggacggct gtagctgccg cttccccgag    180

gaggaggagg gcggctgcga gctgcggcgc gaccagcgcc tgccccccga cgcccacaag    240

ccccccggcg gcggcagctt ccgcaccccc atccaggagg agcaggccga cgcccacagc    300

accctggcca agatc                                                     315


