                         SEQUENCE LISTING

<110>  Beijing Biocytogen Co., Ltd
 
<120>  GENETICALLY MODIFIED NON-HUMAN ANIMAL WITH HUMAN OR CHIMERIC IL2RA

<130>  1

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  4428
<212>  DNA
<213>  Mouse

<400>  1
aagttcctgc tgagcagatc agcctaatgc ttaaatagaa caactcctgg ctgtcattga       60

cattgtctaa aagccaagat gacagactga gaggcctgag cccttgttct ggcattctcc      120

caggaagatg cagtaaaggg gttgacccaa tatactgcag agaatttcat ccagttccct      180

cctccatcct gatcccatgt gccaggaaga tggagccacg cttgctgatg ttggggtttc      240

tctcattaac catagtaccc agttgtcggg cagaactgtg tctgtatgac ccacccgagg      300

tccccaatgc cacattcaaa gccctctcct acaagaacgg caccatccta aactgtgaat      360

gcaagagagg tttccgaaga ctaaaggaat tggtctatat gcgttgctta ggaaactcct      420

ggagcagcaa ctgccagtgc accagcaact cccatgacaa atcgagaaag caagttacag      480

ctcaacttga acaccagaaa gagcaacaaa ccacaacaga catgcagaag ccaacacagt      540

ctatgcacca agagaacctt acaggtcact gcagggagcc acctccttgg aaacatgaag      600

attccaagag aatctatcat ttcgtggaag gacagagtgt tcactacgag tgtattccgg      660

gatacaaggc tctacagaga ggtcctgcta ttagcatctg caagatgaag tgtgggaaaa      720

cggggtggac tcagccccag ctcacatgtg tagatgaaag agaacaccac cgatttctgg      780

ctagtgagga atctcaagga agcagaaatt cttctcccga gagtgagact tcctgcccca      840

taaccaccac agacttccca caacccacag aaacaactgc aatgacggag acatttgtgc      900

tcacaatgga gtataaggta gcagtggcca gctgcctctt cctgctcatc agcatcctcc      960

tcctgagcgg gctcacctgg caacacagat ggaggaagag cagaagaacc atctagcaag     1020

ctagaaaagt cagagcccag gcaagcggat gggaatcaca aagctcaagc caaatctgag     1080

acgccaagca ttcacctaac ggctgtttcc ttctgatccc tgggtttcta gaacattctg     1140

aagtcacagg acataacagc aactctatca ctaaactgga ctttgccatt gaagaatagg     1200

atctaaccac ttcagcacag cagttctaaa gctttaatgg gagagagggc ccaacagtgc     1260

tctgtgtgtt ttgtttttgt gtatatctgt tgatgggagc tgagatggtg tggtcacttt     1320

tcatgtaaca tatagtatag aaaaagtagc tttaggttga cttcattgtt acaacccagt     1380

ttggaaagcc caagtaaaac tcagcactaa tgtaaataat tcctcctcct cctcctctct     1440

cttttcatcc tccgctccat cttcctcttc ttcctcctcc ttttccacct cctctgtccc     1500

tacccacccc cacccatcca ctttccttct tcctttctgc tctcacaagc tcatcctagc     1560

tacacgtgca tggctggctc ctttttcaac ctctgtttgc ctaactggct cttctgattt     1620

catcacttac tgatcagcct ttaaaactct gagctggcaa agatgactct atctatgttc     1680

ttggctcagt cccagaagga aacccccttt tcatgaagct tcagttttga catcctgaag     1740

aacagaaact gtggcagaac aatcttcaga taacatcaaa acaaagtgga gaagccacgg     1800

gaactgtgga gctctggtat tcagaagcct gtgtctaggg tctgcgccag gagcagaagg     1860

ctgaaggaag tcccaggacg tggacttaga tgctttccca gcaggccact ctaagcgctg     1920

gtttctttgg gacagctgtc aattgtacgc tcaatttagc ctgcactaat ctgatgctta     1980

caggtgaaca ctcaaggcac aggtatggac ttggtacata ccgtgaaaac actggaaaga     2040

aaagaatact ttcaagttta cagaaggaag gaaggaaaaa ggaagcagag gtggtgatta     2100

tacaaaagat tagctgtaga ctggatatcc caggcatcct cggataatgc ccccgcccca     2160

gcaccctgat ccaggtcacc aaagccttgt gagatcagac tgcagagcca gtctgtctct     2220

gagtcagtaa atgtagaatt tggatttctc acaagttcct ggcggtgtct tttttttttt     2280

taatattttt tattaggtat tttcctcatt tacatttcca atgctatccc aaaagtcccc     2340

cataccctcc cccacactcc cctacccacc cactcccact ttttggccct ggtgttcccc     2400

tgtactgggg catataaagt ttgcaagtcc tggtggtgtc tttatgctga tctctagccc     2460

acactttgtg aggcactggg ctatcccagt gtgctctcct cttccacaga taccaaaagc     2520

acctgggttt gatgctcaga cttctgagca cgttcttgtt caatctcttg cgtaagattt     2580

cctctcagat gagttgagtc agattctcat gtttaacagt gttttagggg attcacagaa     2640

gcccaaacta tcagttttca tttctgaaaa ggctggaaaa ttttatgaaa aactttcaaa     2700

ggtcagacag agccattttg agtcttttat gtgaccaagt atgaaccaga tctttcctat     2760

ctatggtctc ccctttccaa aatatatctt ttgtggggac acggcaagga ggaaagttaa     2820

atagaatctc aagctactaa ttttagaaaa gaaaaaaata ttaaactctt actaaagagc     2880

tgtgggtagt ggtacacacc tgtaagccca gctctcagga gactgaggca ggaggattgc     2940

agtgagtcag agatcagctt cacctacaag caaaaccctg ccttacccct caacctttcc     3000

ataaaaacag tcttacttgt gtaaaattaa tttttaatac atatttgtgc acgatgtgtg     3060

tgcctggtgc ccagggaagc caaaaaaata tgttcaatgt ccctggaata ggtggttatg     3120

agctgccatg gaggtgatgg gatttaaacc cctgttctct gaaaaagcag ctagttctct     3180

taaccactga gccatctcta cagccccatt aaattgaatt ttattgtcat tactcaatat     3240

gggagatggg gtaatgataa caattttttt tttataatac taagatgttt agctatttta     3300

ctctccttca caagtgtaga gtagaatttt ctagaagcta catgcatgat attatcgctc     3360

tggtgttaac acataatgga tttatcttgt taataaaaga attagtaaat aatattttaa     3420

attttttctt tgttttagtt ttaagatgat taatatctat agatactagt gtacattaag     3480

aaagcctttg ggatcctcaa tcattttgca tggttttagt aattttttaa taacataaag     3540

aaggtctgac agattatgct aaagagctat tgtggtatgg attagaaatg gcccccacag     3600

gctcctgtgt tcaaacatca gctagcagtg ctgctttggg ttttggaacc tttaagaggt     3660

ggggccttgc ttaaggagat aggtcactgg aggtgaacca gacttgcttc cagtctccct     3720

ctctgctgtc ccaggaagcc atcatatgag gagtttcaca acatacttct gctgccacag     3780

agtccctcca gtaaggcacg tactggccac tgtgtagtgt accctctaaa actgagctag     3840

agctcccctc tcctccctac actatctctg ttgatgcttt gtcacggtga tgaagaacat     3900

aagtaaggca aagacaagac atagtttgga gactcacgtg agcatctcag ccagactcag     3960

gcacagctgc gatgtgggaa ttatcaagca tgagatgcaa agcaatggaa aatgaatcgt     4020

tatgacagaa gcctacatct agtcttccct tcttcccatt agtaataata gcccgtgttt     4080

tagaagaaca catctttttg gtgttctagg tagcttatat tgcaaatgtg gcacaatcta     4140

agagaaatct gggatgaggg aacctcagtg aaagattctc cttgatcaga ttgtcccact     4200

gatgattgat atgggaaggc ccaacccact gtgaaaggca ccacccattg gcaggatgac     4260

tggggttata gaagcaaaca ggctgagcat gagccagtga gcaagccagt aagcagtggc     4320

ttctctttgg aagaagcatg gctttcttcc gtggcttctg tttgatctct ctcaatgata     4380

gattgtgacc tagaagtata agctaaaata aaccatttct tacccata                  4428


<210>  2
<211>  268
<212>  PRT
<213>  Mouse

<400>  2

Met Glu Pro Arg Leu Leu Met Leu Gly Phe Leu Ser Leu Thr Ile Val 
1               5                   10                  15      


Pro Ser Cys Arg Ala Glu Leu Cys Leu Tyr Asp Pro Pro Glu Val Pro 
            20                  25                  30          


Asn Ala Thr Phe Lys Ala Leu Ser Tyr Lys Asn Gly Thr Ile Leu Asn 
        35                  40                  45              


Cys Glu Cys Lys Arg Gly Phe Arg Arg Leu Lys Glu Leu Val Tyr Met 
    50                  55                  60                  


Arg Cys Leu Gly Asn Ser Trp Ser Ser Asn Cys Gln Cys Thr Ser Asn 
65                  70                  75                  80  


Ser His Asp Lys Ser Arg Lys Gln Val Thr Ala Gln Leu Glu His Gln 
                85                  90                  95      


Lys Glu Gln Gln Thr Thr Thr Asp Met Gln Lys Pro Thr Gln Ser Met 
            100                 105                 110         


His Gln Glu Asn Leu Thr Gly His Cys Arg Glu Pro Pro Pro Trp Lys 
        115                 120                 125             


His Glu Asp Ser Lys Arg Ile Tyr His Phe Val Glu Gly Gln Ser Val 
    130                 135                 140                 


His Tyr Glu Cys Ile Pro Gly Tyr Lys Ala Leu Gln Arg Gly Pro Ala 
145                 150                 155                 160 


Ile Ser Ile Cys Lys Met Lys Cys Gly Lys Thr Gly Trp Thr Gln Pro 
                165                 170                 175     


Gln Leu Thr Cys Val Asp Glu Arg Glu His His Arg Phe Leu Ala Ser 
            180                 185                 190         


Glu Glu Ser Gln Gly Ser Arg Asn Ser Ser Pro Glu Ser Glu Thr Ser 
        195                 200                 205             


Cys Pro Ile Thr Thr Thr Asp Phe Pro Gln Pro Thr Glu Thr Thr Ala 
    210                 215                 220                 


Met Thr Glu Thr Phe Val Leu Thr Met Glu Tyr Lys Val Ala Val Ala 
225                 230                 235                 240 


Ser Cys Leu Phe Leu Leu Ile Ser Ile Leu Leu Leu Ser Gly Leu Thr 
                245                 250                 255     


Trp Gln His Arg Trp Arg Lys Ser Arg Arg Thr Ile 
            260                 265             


<210>  3
<211>  3216
<212>  DNA
<213>  human

<400>  3
ggcagtttcc tggctgaaca cgccagccca atacttaaag agagcaactc ctgactccga       60

tagagactgg atggacccac aagggtgaca gcccaggcgg accgatcttc ccatcccaca      120

tcctccggcg cgatgccaaa aagaggctga cggcaactgg gccttctgca gagaaagacc      180

tccgcttcac tgccccggct ggtcccaagg gtcaggaaga tggattcata cctgctgatg      240

tggggactgc tcacgttcat catggtgcct ggctgccagg cagagctctg tgacgatgac      300

ccgccagaga tcccacacgc cacattcaaa gccatggcct acaaggaagg aaccatgttg      360

aactgtgaat gcaagagagg tttccgcaga ataaaaagcg ggtcactcta tatgctctgt      420

acaggaaact ctagccactc gtcctgggac aaccaatgtc aatgcacaag ctctgccact      480

cggaacacaa cgaaacaagt gacacctcaa cctgaagaac agaaagaaag gaaaaccaca      540

gaaatgcaaa gtccaatgca gccagtggac caagcgagcc ttccaggtca ctgcagggaa      600

cctccaccat gggaaaatga agccacagag agaatttatc atttcgtggt ggggcagatg      660

gtttattatc agtgcgtcca gggatacagg gctctacaca gaggtcctgc tgagagcgtc      720

tgcaaaatga cccacgggaa gacaaggtgg acccagcccc agctcatatg cacaggtgaa      780

atggagacca gtcagtttcc aggtgaagag aagcctcagg caagccccga aggccgtcct      840

gagagtgaga cttcctgcct cgtcacaaca acagattttc aaatacagac agaaatggct      900

gcaaccatgg agacgtccat atttacaaca gagtaccagg tagcagtggc cggctgtgtt      960

ttcctgctga tcagcgtcct cctcctgagt gggctcacct ggcagcggag acagaggaag     1020

agtagaagaa caatctagaa aaccaaaaga acaagaattt cttggtaaga agccgggaac     1080

agacaacaga agtcatgaag cccaagtgaa atcaaaggtg ctaaatggtc gcccaggaga     1140

catccgttgt gcttgcctgc gttttggaag ctctgaagtc acatcacagg acacggggca     1200

gtggcaacct tgtctctatg ccagctcagt cccatcagag agcgagcgct acccacttct     1260

aaatagcaat ttcgccgttg aagaggaagg gcaaaaccac tagaactctc catcttattt     1320

tcatgtatat gtgttcatta aagcatgaat ggtatggaac tctctccacc ctatatgtag     1380

tataaagaaa agtaggttta cattcatctc attccaactt cccagttcag gagtcccaag     1440

gaaagcccca gcactaacgt aaatacacaa cacacacact ctaccctata caactggaca     1500

ttgtctgcgt ggttcctttc tcagccgctt ctgactgctg attctcccgt tcacgttgcc     1560

taataaacat ccttcaagaa ctctgggctg ctacccagaa atcattttac ccttggctca     1620

atcctctaag ctaaccccct tctactgagc cttcagtctt gaatttctaa aaaacagagg     1680

ccatggcaga ataatctttg ggtaacttca aaacggggca gccaaaccca tgaggcaatg     1740

tcaggaacag aaggatgaat gaggtcccag gcagagaatc atacttagca aagttttacc     1800

tgtgcgttac taattggcct ctttaagagt tagtttcttt gggattgcta tgaatgatac     1860

cctgaatttg gcctgcacta atttgatgtt tacaggtgga cacacaaggt gcaaatcaat     1920

gcgtacgttt cctgagaagt gtctaaaaac accaaaaagg gatccgtaca ttcaatgttt     1980

atgcaaggaa ggaaagaaag aaggaagtga agagggagaa gggatggagg tcacactggt     2040

agaacgtaac cacggaaaag agcgcatcag gcctggcacg gtggctcagg cctataaccc     2100

cagctcccta ggagaccaag gcgggagcat ctcttgaggc caggagtttg agaccagcct     2160

gggcagcata gcaagacaca tccctacaaa aaattagaaa ttggctggat gtggtggcat     2220

acgcctgtag tcctagccac tcaggaggct gaggcaggag gattgcttga gcccaggagt     2280

tcgaggctgc agtcagtcat gatggcacca ctgcactcca gcctgggcaa cagagcaaga     2340

tcctgtcttt aaggaaaaaa agacaagatg agcataccag cagtccttga acattatcaa     2400

aaagttcagc atattagaat caccgggagg ccttgttaaa agagttcgct gggcccatct     2460

tcagagtctc tgagttgttg gtctggaata gagccaaatg ttttgtgtgt ctaacaattc     2520

ccaggtgctg ttgctgctgc tactattcca ggaacacact ttgagaacca ttgtgttatt     2580

gctctgcacg cccacccact ctcaactccc acgaaaaaaa tcaacttcca gagctaagat     2640

ttcggtggaa gtcctggttc catatctggt gcaagatctc ccctcacgaa tcagttgagt     2700

caacattcta gctcaacaac atcacacgat taacattaac gaaaattatt catttgggaa     2760

actatcagcc agttttcact tctgaagggg caggagagtg ttatgagaaa tcacggcagt     2820

tttcagcagg gtccagattc agattaaata actattttct gtcatttctg tgaccaacca     2880

catacaaaca gactcatctg tgcactctcc ccctccccct tcaggtatat gttttctgag     2940

taaagttgaa aagaatctca gaccagaaaa tatagatata tatttaaatc ttacttgagt     3000

agaactgatt acgacttttg ggtgttgagg ggtctataag atcaaaactt ttccatgata     3060

atactaagat gttatcgacc atttatctgt ccttctctca aaagtgtatg gtggaatttt     3120

ccagaagcta tgtgatacgt gatgatgtca tcactctgct gttaacatat aataaattta     3180

ttgctattgt ttataaaaga ataaatgata tttttt                               3216


<210>  4
<211>  272
<212>  PRT
<213>  human

<400>  4

Met Asp Ser Tyr Leu Leu Met Trp Gly Leu Leu Thr Phe Ile Met Val 
1               5                   10                  15      


Pro Gly Cys Gln Ala Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 
            20                  25                  30          


His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 
        35                  40                  45              


Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 
    50                  55                  60                  


Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 
65                  70                  75                  80  


Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 
                85                  90                  95      


Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 
            100                 105                 110         


Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 
        115                 120                 125             


Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 
    130                 135                 140                 


Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 
145                 150                 155                 160 


Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 
                165                 170                 175     


Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 
            180                 185                 190         


Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 
        195                 200                 205             


Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 
    210                 215                 220                 


Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Gln 
225                 230                 235                 240 


Val Ala Val Ala Gly Cys Val Phe Leu Leu Ile Ser Val Leu Leu Leu 
                245                 250                 255     


Ser Gly Leu Thr Trp Gln Arg Arg Gln Arg Lys Ser Arg Arg Thr Ile 
            260                 265                 270         


<210>  5
<211>  3886
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5 arm

<400>  5
ttcttaacag cttggtgagt gccacttcag tgagtgtgtg tcttcaataa tcacaatatt       60

ttctttggaa acatgactat tcgagtgttt gttcatctat cgattcagtt gccctttagt      120

gttgctatgt aggtttttgt gtattctgga aacaaatatt gtcatatata cattataaat      180

ggttttctca cagcctgcca catctcctcc attttctcat ggtctttgga ttagctgata      240

cttttgtaat tttttttttc ttttataaaa tctttaacct ggagcagggc atgggagggt      300

ggactcagac cctctgatgc tggggttaca ggtgcttgtg agctgcttgg ttcaggtggc      360

tgggaagcga acattggtcc tctgcaaagg tagcaagcat tctcagcaac tgagccatct      420

ctccagcccc cttttccttt tatattcagt gcatgctctg tcctaagaaa tcttcaccta      480

tccacaactt ctaaagctgt ctgcctttat ttccttttag aagttttaca gttttaattc      540

tatgattgca tgtgatacac acacacacac acacacacac acacacacac acacacacac      600

gttcttatta tggaagagaa ataagttttc tctgtggaag gaagctcttt tcccttttca      660

catttatcca cacgactcaa gagtatttgt tgagaacgtt gtcctctttt catgaattgc      720

tttggtaaat gggggggggg gcagtaatta aatgttcctg agtttctaga atgtatattc      780

tgtcgctgta cttgtttttc tcccagttgc ttgctgtctt gatggctagc tttatagtaa      840

cagatgttat gagtctccta tcatacactt tatcttacag ttatcttaga tacttccaag      900

gaagtttgaa aacaattttc caattttact aatatgtaca tcaaaccagt gaattgaagg      960

taaactgaca tgtcttttaa aaatcagccc tccaatttat aaacagtgta tgtttctctg     1020

tttatttcac ttaaaaattt tttctcagca gaaattcttt tgcaattttc aagtctgttc     1080

ttgtaggtgt gggtgaattt attcttgagt attttatgat tctgatgtgt aattacaatt     1140

aactttagat cttaattaat tcattcagtt taaatatata acaagtgatt tgagatagcc     1200

acacctacct acttacatac gccattatgt agaattctcg gtactttcat ggaaagaggc     1260

atttttagct gtgttaagtg ccctttccca caaaactact ttctgatagt aacatagccc     1320

atatcagctt tcctatgctt atctctgcat atctttttct attctttcac tcaattcatt     1380

ttggttttta tattttagca ttttctttgt gagatagtat gcagttgttg tggtctgaaa     1440

gtttctattt tagaaaaaat tgttcattta tgtttactat aatcactagt agtgggttga     1500

cgttgatgtc tgccttcttg ctctttcttc gtgcttcttc atttcaccta aacagatctg     1560

tttaatacat attggaatgt tctgtttaat ttattctgtt atcttttaat gtgtgctcct     1620

ggtagtattt cattattggg tgccttccct ggaatattta ctatgcattc ttaattttct     1680

atgttctatt tagttagcat tgtaccattt cagacatgct agaaaaattt aaaaagctaa     1740

aggcccatca acctcagctt tcttgcccaa gttgtcacgt tttacattta agtaggtact     1800

aagatctgtc ctgtagggac cagcacggtg gctcagtggc taagaatgca tgcacactgc     1860

tcttccagaa gatctgagtt tagttccagg cactcacact gtacagctca caatcacctg     1920

tttctgacgc cctcttctgg tctctaggag aactgcactc accccctccc ctaacacaca     1980

cagttaaatc aaaaataaat aaaagaccta ccatacagtg ctattttttt tttattttag     2040

acatctcttt caagaaaaaa ctggaatttt ctcatgaata tatacttact aatgcccttc     2100

attattccca gtagatttct atttttgact aatataattt ccctgggtct tgacattttg     2160

tccttagctt tcttttcact tttaatatgc actataagtg tgctagtggg gttttgtgaa     2220

ctttctttta cctgaaaatg tgctaacccc atcctcattt ttgaagggtg tcacgtctaa     2280

tcaggattga gctgaaagcc tgtctgtgtg tctgtttcga ggctggctct gtttccctgt     2340

tattttctgg ctccctttgc tctgtggtga agtcagttgt catccatcac tgttcccact     2400

aatgtgtgtg gcctttccct ctggctcctt taagacattc tctttagatt tttggcagct     2460

tggcattaga tgcataacaa tttgctttta aaagtctact ctggtgtcat gaacatcttg     2520

gatctgtaag ttaacatctg tcttcaagag tcagaaatga gctggcaaga tgggtaagtg     2580

tgtacaggtg tttcctattg gtggccctcc cacatttcat ccttcccaaa cagttccacc     2640

aagcaggacc aagcattcaa atatataagt ctatgaggga cattttcttt cgaaccacta     2700

aacagaggga tagaaaaggg ggcggggact ggatgtgtgg gttggggtga tagatagaac     2760

atacttcttt gccttctact tcctgtccca gctacatctc cagctgactc ttgggtgcct     2820

gcccacactg agggcggggt cttttcccta ttattttgct gacctgtagt cagtcttctc     2880

aggcaatgtg cttacagata cacccagaag tatgtttcaa attctctagg catccattag     2940

agccttcagt cgacacctca aactagccgt catgatactc accagtctaa tgtataagca     3000

ggtgcagtgt tggcctcggt gttggcctca gcatgccgtt ccagatggag caggtgggca     3060

ggatggcagc ttgattacaa ccaccttcac acactcagcc atcatgccat gacagccccc     3120

tgctttcctg ccactcccac tccttaccat cactcactcc cccctccacc ccccacctgc     3180

tgtggtagtc tcctagtcac ctgatgtctt caagcacaag agcttgttgc aatggaatgg     3240

ttttcactta catcagggtc cctcttccct tcaagagtcg ggcatagaga gctggctgtg     3300

gcagtgtgca cctgtaatcg aaagacttgg gactgaaagc aggatggtag gagttcatcc     3360

tcagctacat aacgaacttg agcccaattg ggcctacagg agaacactcc ctctcaaaat     3420

gattacatgg gaacctctat ttctggtgat catgctagga cacctgacct gtctcagaag     3480

ctttggccca cccaacctct ctcctgtgta tgctagacac aaagtacctt cagagtgaaa     3540

ttctacttat ttgtacttat tccttctaca gtttgcattt tatactgtag aggaacttga     3600

gtgaacactc ctatagcatc tctgacacaa atatttatct cttcagtttt atacaatggg     3660

tttcattgtg acttttccat agatgcatat aatgtatttt gatcatattc acacacacac     3720

acacatacac acacatacac acatatcatc ctctgtgctc ccactgatcc cgaggaggta     3780

gagtacgtac caatccttta aattgagaga agaagcatgt gagcaagcta ctgcacaatc     3840

aaactcatgt ctcaccaaca ttctccttgc ctttgcagaa ctgtgt                    3886


<210>  6
<211>  5776
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3 arm

<400>  6
gccagaacga catagtgaga ccttgtttca aagaaataaa agaattttta aaaagagaaa       60

gataaatgtt tgttcccagc ttactctcct ttttatggaa cctatggaat ggggccccac      120

atttagagca gtctccctac ccatttagcc taatcttgat aacctctcaa ggacgtgtcc      180

agaacgttat ctctaatgtg actcgaaaac ctgtcaatca acataaacca agagcatcat      240

cagagttaaa tcacccaaaa ccagtttctc ccacatacat tttcaagaac aatgcctccc      300

aaactttggt gagagacaaa gcaaggatcc agggagagaa aatatagtgt ccatacgcac      360

ctcagttatt cctggtagga ggcaaaagca ggccttcctg aaccacggaa tcaatggaag      420

cttctggaag attcacatgt cagcatttgg gaagtgtgag cacttccagg cgatatcggt      480

gtgtttagga tttcttatct tgtttcaata gttcctttat gaaggatgat gctgctaatg      540

aggactatcc tctgggcctt cctcaggttg agctgttctg tcaagtacca gcctctttct      600

atggctgttc cttctatgcc ccctctgctc tttgaaggct ccttgatcct tctaagaaat      660

acccagggag aagaggaaag aaagaagaaa ctgcaggccc cagctgttca ggaggtcctt      720

gatcacctac agaattttct cacctgatct acagatccaa cccccgatcc atgcattccc      780

aaagcatggt cctgacctga actccatctc cctctcttga cagtggccag ctgcctcttc      840

ctgctcatca gcatcctcct cctgagcggg ctcacctggc aacacagatg gtaagtccaa      900

cactggaagg acccagcacc ccagtgtctg gtccctggcc tggcatcgtg aacattgcct      960

agagcttgac ctcagctgac acgctgggaa gatggctccg acgaaagcat gagatgggtg     1020

tgcaattcct aaaagctttg tttaaaaaaa aatcgaatgt gatgatggca tttgcctata     1080

attgtataat tcaagagctg aggaggcagg cagaggtgga tccctcatac tctctggcca     1140

gccagcctag agaaactggt gaactccagg ttgatcagga gactctattt caaaagtaat     1200

gtgaataata tactaaggaa caagattcca ggttgacctc tagactccac agtcatttac     1260

tccctgacac acgtactctt actgaatgtg tatgcccggt actgagtggg ttacattagc     1320

aaatgtcacc cctactctca gaggtctgtg ctgtttagag attaggaaaa cctaggcttc     1380

ccgaatgtgc agatagcaag agtttgaatc ctatttagag atcttcaacc ttgccatcca     1440

tccaccaggg ctctaccaag gtcgctggaa cagaccatgc caccccaccc tccctccaac     1500

tccgctgatc atccactaga aaccagcatg ggtagcaagg gatggagaga ggagacgggg     1560

accatggcta gaccaggctt gatggtatgg atgaaggttg ctagatactc acaaggcttt     1620

aaaaaaaaca ttcttgcatt tatcattaaa tacagtgaaa agctcccgga tagcaagagc     1680

agggggaggc tctgtattct gtgtctgtgt gtctggctgt gtctgtgtgt ctgcctgtct     1740

gggagttccc ttgtttttta ggccctaagt atcttggtca gtgatatctg ccatggctca     1800

tataatgtct ttttatggat aaacatattt tcaagccatg attaagtaaa gagttttgtg     1860

tgagacagaa accagaaatc agaaatctag gtgaacatac aaattgcacg tgcatgtgtg     1920

cacacacaga cacacacaca gacacacaca cagacacaca cacacagaca tacacagaca     1980

cacatagaca cagacataca cagacacaca aacacacaga cacacagaca cacacacaca     2040

cactgggggg ttggaggagg actgtgtatc atggtacatt ccattgatct cagcacacaa     2100

gagttggaga caggaagatc atgagtttga gtttgaaagc agtcgggctg tatagaaatt     2160

cgaatgccag cctcatagtg agagctgtct caaagtaaat gagtaaataa gatgccaagg     2220

gatgtagttc atttggtaga gtgagagctg agcatgccca cgaagcccgg acttgatctc     2280

agcaccataa gaaaccaggt atagcggcat aagcctctaa taccagcgct caggaggtag     2340

aagcagggaa atccaaagtt caaggtcacc ctgggctaca tgagatctta tattaaaaaa     2400

ctaaacacat tattttttaa agatttattt atttatttat tttatgtgag tgcactatag     2460

ctgtcttcag acacaccaga agcgggagtt ggatcccatt tcagatggtt gtgagccacc     2520

atgtggttgc tgggaattga actcaggaat tctggaagag cagtcagtgc tcttaaccac     2580

tgagccatct ctccagcccc aatacattat ttaaagaaga agaagaatta gaagaagaag     2640

gagaaggagg aggaggagga gaagaagaag aagaagaaga agaagaagaa gaagaagaag     2700

aagaagaagg aggaggagga ggaggaggag gaggaggagg aggaggagga ggaggaggag     2760

gaggaggaga aggagaagga gaaggagaag gaaggagaaa aggagaagaa gaagaagaag     2820

aagaagaaga agaagaagaa gaagaagaag aagaagaaga agaagaagaa gaagaagaag     2880

aagaagaaga aagagttaag agtataaaac catcttgaga aaagccagat acatcctcat     2940

tagagtcagc tgtgtgagat gttctgtccc tgtctttgtt tcatttcctg tgggtgatat     3000

aaaatacccc aaccaaagca gcttaagaga gaaggggctt ctctgactta caggtccagg     3060

atgcagtcca cccttgtgtg gcagctaagg tggccgaaac ccgaagcctt tactcatatc     3120

acaccctcca tcaaaagcag ggatcaatga atttgcatgc acacttatcc cagctcactt     3180

cctctttcta tacagtctag gacccaaatc catttagact gggttttccc acatcgatta     3240

cagtaataaa aacaatcccc tgcaggcatg ctcacagatg acctaatcta ggtaatccac     3300

cattgagatt cccttccggt aactttaggt tctgttaaat tggcaattaa cactaaatca     3360

tcacacgcct gacgtcaagg gtgtgcccct cctgcccgag caggttctgg ccagccctga     3420

tactttaaat ctttaataac agagtcatag gtatgtgggt gagaatccag tttcttccat     3480

tcttttgttg ttcatctggc ctccttctca ttttcctcaa gtggagcaag aagcagaagg     3540

cattgaggtc cacatgaggg taccccgata gttgctgtgc taagagttac tcatggccct     3600

gacactctac ccatttcctt aagatgttct tttagagttc tgggagataa aacgtgatgt     3660

aatatgcctt gacagtctag gcccctcagt ccttgtgaga ccttcttcct gctcaggacg     3720

agcatctacc tggcatggca ttttcgtttg gtgaagacac cagactcaga accagaggac     3780

agggctcttc atgagcaaca atagcttaga gtttacaatg tgaagcaagg agctgtctaa     3840

gactgtgaga ttcccctaga cctcccagta gcggggccat tagggagact gaaatagaat     3900

ctcaatatga cccctttggt caaagaattt gtccaagaaa acaaaacaaa acacttctga     3960

aatcccagcc acaaggagtc cagcataaca aggtcctgtt gcagacacat aggtgcctgt     4020

gctcacgttt gtacatatat atatatttac atgtgtgtat atatgtgtgt atatatatga     4080

aaaaagttta ctttctagaa gctaatttca tctaatgctg ccctcttgtg gccaattgga     4140

aatcttacct ctgaattctg aaaatttcag tctttgccac tttggaacac ttaccaggag     4200

ctaggagggc actggctggc tcacattagt tacgttttta ggaatttcat gggttttgtt     4260

ttgtttcgtt tcttttcttt gtttgtttgt ttgtttgttt gtttgtttgt ttgtttgttt     4320

gttttgagac agggtttctt tgtgtagccc tggctgtcct ggaactcact ttgtagacca     4380

ggctggcctc gaactcagaa atccacctcc ctctgcctcc cgagtgctgg aattaaaggc     4440

gtgcaccacc atgcccggcc tcattttttg ttttaaatgt tcgccctggg aatttaatcc     4500

aaggccttga gcatgctaga cacatgttct attcagctat gatccagccc tttgaactgt     4560

tttgttaata cttttctctc tttttctgtt tcgtaatgtt gatttttggg tgggattgga     4620

gcacgagcat ccggaagtta gaggacaagt tttgagagtt ggttttctcc ttcattcact     4680

aggattggtg ccaaactccc ttactccctg agacatcttt ctcccccagt cggattgtta     4740

ggttttgtgt gtttgatttg gtttagagca gtagtcctta gccttcctaa ttctgtgacc     4800

ctttaatata gttctgtatg tttaatggct aatcttactc caactcagca gttattagtc     4860

cacaggacta aatgaggaaa aatccacaac tgccaagtgt cagacattgc accgcaggcc     4920

acagacatgc acaacccagc cctgctcgta ggaactagta gactggaaga aaaaaaaaac     4980

ttgaaatatt tttgtggtgc tgaggataca ggtcaatagt agaatgtttt ctcagccctg     5040

ggttcaatcc ttagtataga agaaaaagaa gaaagggaga tatgggtgga aggaaatacg     5100

gagggaggaa gggaatgagg gagggaggga gggagggaca tttgttgaaa gtctaagatg     5160

gagatatgta agcagaagga catttaatct acatgggaat caaaagacag ctgactgagc     5220

tggtatactc tgaagtgtca gataaaacga gccaggaaac atctcctgga agaggaagct     5280

ggggcaccta tggaattgtg aatctgtgtg gtgatgtctg agagaaggtt gagttgtagg     5340

acataaaatg aatgggcacc atggccacaa tgataccaca ctgcccagac agtcagaaat     5400

actgtcatgg aagatgaggc cgcattgagg agctgggcca agatgctgat ggtggtggca     5460

cctcagatca cctcacctag gagagaaacc gttatcttgg ccttctgggt gatctgctgc     5520

gtagaggctt accgtgggca atgtgttgac aatatgattg cattatggtg gaataaatcc     5580

acatagaagt tggtgactgt gcttttgtgg gtctgtggat ctgagtgaat ggaggaaaca     5640

aaccaagggg tatagaaaag aattgagcaa attagtatga ctggtaagct ggataaaaag     5700

atggagtatg gaagaaggat gagtcagaga ggggccgtgg gtttctcaga cagggagcct     5760

ctgtagccac tacatc                                                     5776


<210>  7
<211>  6574
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotides from the position 6019444 to the position 6026017 of NC_000010.11

<400>  7
gacgatgacc cgccagagat cccacacgcc acattcaaag ccatggccta caaggaagga       60

accatgttga actgtgaatg caagagaggt ttccgcagaa taaaaagcgg gtcactctat      120

atgctctgta caggaaactc tagccactcg tcctgggaca accaatgtca atgcacaagc      180

tctggtaagt gtcccttctg tgactaccaa gaacaaaaga actgcagcaa agagtgagac      240

agagcccagc ttttgttcac tcagccctat agacacaaat gggccagtcc agtctacagg      300

ataactaatt ttagtggttc ctgaaaagtc attcactaaa cacaattttt tttttttttg      360

gagacagact ctcactctgt tgcctaggct ggagtgcaat ggcacgcgat ctcggctcac      420

tgcaacttcc gcctcctggc ttcaagtgat tctcctgcct cagcctcctg agtagctggg      480

attacaggca ccctccacca tgcctggcta atttttgcat ttttagtaga gatggggttt      540

caccatgttg gccaggctgg tctcaaactc ctgaccttga gtaatctgcc catctcagcc      600

acccaaaatg ctgggattac aggcatgagt cacagtgccc ggccacacaa ttttattttt      660

atttttgaga cagtctcgct ctgttgccca ggctggggtg cggtggtaca atctcagatc      720

actgcaacct ccacctcctg ggttcaagtg aatcttgtgc cacagctgcc caagtagctg      780

ggactacagg tgtgcaccac catgcctggc taattttttt atttttagca aagatggggt      840

ttcgccatgt tggccaggct ggtctcgaac tcctggcctc aagtgattct cctgcctggg      900

ccttataggc gcacaccacc atgcctggct aatattttgt attttttgtt agtgatgggg      960

tttccccatg ttgtccaggc tggtcttaaa ctcctgggct caagcagtcc tcctgcctca     1020

gcctcccaat gtgctggaat tataggtgtg agccaccaca cctgacctag acaccctctt     1080

ttatcctgag ctggaatact gatcaccccc ctcactgggg cactcagact caactccttt     1140

gccaaactct tcacaataga gagggctgtt ctagaagttt ccttgtcatt gtgttaagat     1200

ggcgtctcta acttcaagca ttctgaacag ccttagggag caaaggatca gtgtgttgtg     1260

gagtgagacc actcctgctg ctgtcaaaca acacagagac aattgacagc aagcaaggag     1320

tcccttttgg tgagagacac acacatgcac acataacata catgacatac acagacacgt     1380

aacatacatg gcatacacag acacgtaaca tacatgacat gcacagacac gtatacacac     1440

gctcaaaaac atgtcgtggg cattgccata tgcacttgaa tacccagagg ccggtgctgg     1500

gagggcatac tctggtttcg tatttgtaag ctcagcagcc agacaccacc agacacatcg     1560

tgggccctgg acaggtgtgc ttctcaagtg aatgaataca tgaacagtgt tttctatgaa     1620

gcatttgtga aattatctgc atcattccat ctatattttc tagccactcg gaacacaacg     1680

aaacaagtga cacctcaacc tgaagaacag aaagaaagga aaaccacaga aatgcaaagt     1740

ccaatgcagc cagtggacca agcgagcctt ccaggtgaga gatgaatctg tcctccagct     1800

aactcctgct agcgcaccct tctcctgcag gcagatgatg tgtacccaaa ggaagaaggt     1860

ctgccctgcc ttctataaac acatgcatgt ggctccgtcc tcccaagaca tcattgcaga     1920

caagaattag tgcagagttg ctcagaggct gtttgctcag caactaaagc caatctgacg     1980

gcagccaatc caggtctggg cttactccct ggtttggtaa ctggccaaat agaattcaac     2040

ccaaattcaa ctcaacttaa aagcgtctgt tgggtctgtg tgccaggccc tgctgataga     2100

agacacaaac aagagtgtga caacgatccc tgccctcaag gaaacgctct gcgtcttccc     2160

cttaagcaca agacaacgca ccgcaaaaaa attacttgct tcccagtatt tcttccaggg     2220

caaggttgta aaatgtgcat gccacacttg tgagtctcaa aagctccaga tgcgtgtgtc     2280

ctggccagca gtctgtgcat gagtgggcac tgtggctgtc ccttgtctgc cctctgcttt     2340

cccacgactg aattgggatt cagttatggt gtcaagaaaa tgggctgggc gcagtggctc     2400

acgcctgtaa tcccagcact gtgggagtct gaggcaggca gatcacttga ggtcaggagt     2460

tcgagaccag cctggccaac atggtgaaac cttgtctcta caatcataca aaaattaagc     2520

tgggcatggt ggtggatgcc tgtagtccca gctactcagg aggccaaggc atgagaattg     2580

cttgaaatca ggaggtggag gttgcagtga gcagagattg catcactgca ctccagcctg     2640

agtgacagag gaagactctg tcccccctca aaaaaacaaa acaaaaaaga aaaaaaaccc     2700

acagaacctg ctaggaagaa ttgagctttc taaggtcagg ctagccttag gagacttcag     2760

agccctttgc tgattttaca accttacaaa gaactgtatc agataccatc ccatttgagc     2820

ctggctatac aatgagtcag gaccaggaag ggttacttca acttactgaa gaaactgaga     2880

tgcacaaaag ataaaaagat ttggttaagc tcacatagta gggctggaat cagacctcag     2940

gtctcccaga ttagatgagg aaactttctg ctgattactt attttaaaag agtttgaatt     3000

tacttacagc ttccattatt ttatttttta aaacagacta atctaggggc acagttgcaa     3060

catgatgctc ttactccttc cccttccgac tttgtcctgg aatggtgttt ccaagcacat     3120

ttgtttgttt tgttctactt cccccatatc accctgcaca gttattggaa ggcttcaaag     3180

aggccaatgc ttgttgtcct ttctcgaagg ctggctctgc ctgagggatg tttcaaagat     3240

tgtctcagtt tgggtgtttt cattggaaag aagcaaaccc aattcaaaca tgttcagaca     3300

gttacgaatt gtactggctg tcaaacaacc ttttatcctg agaccaaaac acaagttcag     3360

tcgtgatgtt caaggtgcca catggcagga gccagcctgg gacagggcca cccagcttcc     3420

tgctggggaa gaggctctgg aggatattta cagaggctct gttgggtatg cagggttggg     3480

gagagtctgg aagttcagag cacgtgcgca gtttcacagg ctggggttag agggcacata     3540

caattccata tgtccaggtt gggggctgtg ggtggggaat tcagagattt aatcagaaca     3600

agacatggtc tcaggccaca ggtccgtcag gcctgggatg tagttgggtg agtggaaagt     3660

tctggggatg ggaaattttg agacgtcttg aaagggcgca tgtgtgtcaa ccttcaggtg     3720

tggcttgatc agagatggtg ctccatttct ctgaggttcc ctcagcccgt ccctcctcct     3780

ggctttggtg ttgtccccaa tccttccgac atcatcccaa gatgcccagc cgctgccccc     3840

aagaatgcac ttcctcactt gtccagcaga aatccaaggt ggtcttcatg ctccattggc     3900

ccaaataggc ttgagcctac ccttgtgtct cagctgagac tgccccgccc actcctgtcc     3960

actgtcaaca cctgcactgt cttccaagag gcagttgtgg cgttcctgtc tcggggccgc     4020

accaaccaga atgaatcgct cattcctctg tgttcccatt gccaacgtct gctatccttc     4080

acatgccttt cttacccaat ccactgtcca ccccttgcag gagtgacagg gtcttcgtgc     4140

cttcctacag ggcctagcaa acttccccac gctcagacgt tctcaacata tattggttga     4200

atgaaaggat gagcaagcag tcaagagagt aagaaccaag gtccagtccc tagcaagagg     4260

caacctggac tcactcgcgg tgctgtcccc aacttgcctt cagagcattc cttccatctt     4320

ccaggtcact gcagggaacc tccaccatgg gaaaatgaag ccacagagag aatttatcat     4380

ttcgtggtgg ggcagatggt ttattatcag tgcgtccagg gatacagggc tctacacaga     4440

ggtcctgctg agagcgtctg caaaatgacc cacgggaaga caaggtggac ccagccccag     4500

ctcatatgca caggtgaaat ggagaccagt cagtttccag gtagggtggc tcccttctgg     4560

ggtccacaat gttgctttgc tccatcaggc tgaccagagt ggaatccctg accactcctg     4620

ctggacaaga gtggtccttg gacaagaccc ttgatctccc tgggcttcca ttttttctca     4680

ttctgagaaa aaaaaaaaaa tttaaataga ccagatcaga tggactctaa gggctccttc     4740

aatgaaggtc cccctgtgcc ctgagtgggg agaaaggttg gagacagaag agaaggaaag     4800

ccctctccta agcaccctag atgctgcctc gtagttggtc ctaagtccac tacttctcta     4860

agccaccaag gcctcctctg gctccaaggg caagtccagt ttcccactca cacatttttc     4920

ctcttactgg tgatcgtgta aatgcatgat ctgaacagcc ctccacggtc agcatgttcc     4980

ttccccttgt gccattcccc agctgagctt gtaaaaataa acataaaatg tgcagagtcg     5040

ggacttactc agtgcacatg cgcgcacaca cacacacaca cacacatact catgcaaatg     5100

ctcagcccac cgatgtcgag gtctcctgct gtcttccttc tgaaatctta ttagtatcac     5160

gacaatcaaa agtgacttac atgcaggctg ggcacagtgg ctcacacctg taatcccagc     5220

actctgggag gccaaggcgg gcagatcact ttaggtcagg agttcgagac cagcctggcc     5280

aacatggtga aaccccgtct ctactaaaaa tacaaaaatt atctgggcgt ggtggtgggt     5340

gcctgtaatc ccagctactt gggaggctga ggcaggagaa tcacttgaac ccaggaggtg     5400

gagttgcagt gagctgagat cacaccactg cactccagcc tgggcaacag caagactccg     5460

tctcaaaaaa aaaaagtgac ttacattcaa ccacaagtat tcctggtttt cttgctcact     5520

gcacttctca tactccatgt ccttctcttg ggttccctag ggaggctgtt ttgttccctt     5580

agctgcttcc ctgggccacc ctgacagcca gggaatgctt gctgcttcct tggaagaggg     5640

tgagaggcag ggcagagcca gggttgctga cccctcagga gaagtggaga gcctggcaca     5700

gaagcagctg tcccttagtg catctctgct ccatccagtg gttcaatggc attcgtatta     5760

ttatgacctg tttccaagtc acagatggaa accaggactg gggagggagg ctggggagaa     5820

agcattgctc agagttttgg ggacagctag ggtcagagtt agaattcagc ctcgtctgct     5880

ctgctgagac cacttcaccg cttcccagtg cccctgctgt ggaaccatga ggggtggcat     5940

ccctggcgaa gtgcccacac caggattcct gcctcctcgg gcgtgtctgg ggccggctgc     6000

ctggggcagg cggggtggga gtgaggccct gactcctgtg tttagctcca ccagcatcac     6060

ttactctctc ccccaggtga agagaagcct caggcaagcc ccgaaggccg tcctgagagt     6120

gagacttcct gcctcgtcac aacaacaggt gcgggagaag acaaacgctg gaccacagag     6180

gcctagtcca aaagggcaga ggtgaccagg agccaggctc agggagatag gcggaggtga     6240

cctgtagggg agaagcccac agcagcctcc tctccctctg agcagggaca gggcctcagc     6300

aacctgcagg ccccaaagca agtgtcagaa agagggaacc caggaccagg accaagcacg     6360

gtccccaggc agagatggaa caccttccct caccaccacc acgtgtctcc caccagcctc     6420

tgagcttctc attcacagag acaccctgac ttcctttagc ctcgtgctgt cctaaagtca     6480

cggtagcagg agtgtctctc tttatctctt tttcacagat tttcaaatac agacagaaat     6540

ggctgcaacc atggagacgt ccatatttac aaca                                 6574


<210>  8
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The connection between the 5 end of the human IL2RA gene sequence and the mouse IL2RA gene

<400>  8
tctccttgcc tttgcagaac tgtgtgacga tgacccgcca gagatcccac                  50


<210>  9
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The connection between the 3 end of the human IL2RA gene sequence and the mouse IL2RA gene

<400>  9
catggagacg tccatattta caacagagta taaggtagca ggtgggccag                  50


<210>  10
<211>  4440
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The mRNA sequence of the engineered mouse IL2RA after humanization

<400>  10
aagttcctgc tgagcagatc agcctaatgc ttaaatagaa caactcctgg ctgtcattga       60

cattgtctaa aagccaagat gacagactga gaggcctgag cccttgttct ggcattctcc      120

caggaagatg cagtaaaggg gttgacccaa tatactgcag agaatttcat ccagttccct      180

cctccatcct gatcccatgt gccaggaaga tggagccacg cttgctgatg ttggggtttc      240

tctcattaac catagtaccc agttgtcggg cagaactgtg tgacgatgac ccgccagaga      300

tcccacacgc cacattcaaa gccatggcct acaaggaagg aaccatgttg aactgtgaat      360

gcaagagagg tttccgcaga ataaaaagcg ggtcactcta tatgctctgt acaggaaact      420

ctagccactc gtcctgggac aaccaatgtc aatgcacaag ctctgccact cggaacacaa      480

cgaaacaagt gacacctcaa cctgaagaac agaaagaaag gaaaaccaca gaaatgcaaa      540

gtccaatgca gccagtggac caagcgagcc ttccaggtca ctgcagggaa cctccaccat      600

gggaaaatga agccacagag agaatttatc atttcgtggt ggggcagatg gtttattatc      660

agtgcgtcca gggatacagg gctctacaca gaggtcctgc tgagagcgtc tgcaaaatga      720

cccacgggaa gacaaggtgg acccagcccc agctcatatg cacaggtgaa atggagacca      780

gtcagtttcc aggtgaagag aagcctcagg caagccccga aggccgtcct gagagtgaga      840

cttcctgcct cgtcacaaca acagattttc aaatacagac agaaatggct gcaaccatgg      900

agacgtccat atttacaaca gagtataagg tagcagtggc cagctgcctc ttcctgctca      960

tcagcatcct cctcctgagc gggctcacct ggcaacacag atggaggaag agcagaagaa     1020

ccatctagca agctagaaaa gtcagagccc aggcaagcgg atgggaatca caaagctcaa     1080

gccaaatctg agacgccaag cattcaccta acggctgttt ccttctgatc cctgggtttc     1140

tagaacattc tgaagtcaca ggacataaca gcaactctat cactaaactg gactttgcca     1200

ttgaagaata ggatctaacc acttcagcac agcagttcta aagctttaat gggagagagg     1260

gcccaacagt gctctgtgtg ttttgttttt gtgtatatct gttgatggga gctgagatgg     1320

tgtggtcact tttcatgtaa catatagtat agaaaaagta gctttaggtt gacttcattg     1380

ttacaaccca gtttggaaag cccaagtaaa actcagcact aatgtaaata attcctcctc     1440

ctcctcctct ctcttttcat cctccgctcc atcttcctct tcttcctcct ccttttccac     1500

ctcctctgtc cctacccacc cccacccatc cactttcctt cttcctttct gctctcacaa     1560

gctcatccta gctacacgtg catggctggc tcctttttca acctctgttt gcctaactgg     1620

ctcttctgat ttcatcactt actgatcagc ctttaaaact ctgagctggc aaagatgact     1680

ctatctatgt tcttggctca gtcccagaag gaaaccccct tttcatgaag cttcagtttt     1740

gacatcctga agaacagaaa ctgtggcaga acaatcttca gataacatca aaacaaagtg     1800

gagaagccac gggaactgtg gagctctggt attcagaagc ctgtgtctag ggtctgcgcc     1860

aggagcagaa ggctgaagga agtcccagga cgtggactta gatgctttcc cagcaggcca     1920

ctctaagcgc tggtttcttt gggacagctg tcaattgtac gctcaattta gcctgcacta     1980

atctgatgct tacaggtgaa cactcaaggc acaggtatgg acttggtaca taccgtgaaa     2040

acactggaaa gaaaagaata ctttcaagtt tacagaagga aggaaggaaa aaggaagcag     2100

aggtggtgat tatacaaaag attagctgta gactggatat cccaggcatc ctcggataat     2160

gcccccgccc cagcaccctg atccaggtca ccaaagcctt gtgagatcag actgcagagc     2220

cagtctgtct ctgagtcagt aaatgtagaa tttggatttc tcacaagttc ctggcggtgt     2280

cttttttttt tttaatattt tttattaggt attttcctca tttacatttc caatgctatc     2340

ccaaaagtcc cccataccct cccccacact cccctaccca cccactccca ctttttggcc     2400

ctggtgttcc cctgtactgg ggcatataaa gtttgcaagt cctggtggtg tctttatgct     2460

gatctctagc ccacactttg tgaggcactg ggctatccca gtgtgctctc ctcttccaca     2520

gataccaaaa gcacctgggt ttgatgctca gacttctgag cacgttcttg ttcaatctct     2580

tgcgtaagat ttcctctcag atgagttgag tcagattctc atgtttaaca gtgttttagg     2640

ggattcacag aagcccaaac tatcagtttt catttctgaa aaggctggaa aattttatga     2700

aaaactttca aaggtcagac agagccattt tgagtctttt atgtgaccaa gtatgaacca     2760

gatctttcct atctatggtc tcccctttcc aaaatatatc ttttgtgggg acacggcaag     2820

gaggaaagtt aaatagaatc tcaagctact aattttagaa aagaaaaaaa tattaaactc     2880

ttactaaaga gctgtgggta gtggtacaca cctgtaagcc cagctctcag gagactgagg     2940

caggaggatt gcagtgagtc agagatcagc ttcacctaca agcaaaaccc tgccttaccc     3000

ctcaaccttt ccataaaaac agtcttactt gtgtaaaatt aatttttaat acatatttgt     3060

gcacgatgtg tgtgcctggt gcccagggaa gccaaaaaaa tatgttcaat gtccctggaa     3120

taggtggtta tgagctgcca tggaggtgat gggatttaaa cccctgttct ctgaaaaagc     3180

agctagttct cttaaccact gagccatctc tacagcccca ttaaattgaa ttttattgtc     3240

attactcaat atgggagatg gggtaatgat aacaattttt tttttataat actaagatgt     3300

ttagctattt tactctcctt cacaagtgta gagtagaatt ttctagaagc tacatgcatg     3360

atattatcgc tctggtgtta acacataatg gatttatctt gttaataaaa gaattagtaa     3420

ataatatttt aaattttttc tttgttttag ttttaagatg attaatatct atagatacta     3480

gtgtacatta agaaagcctt tgggatcctc aatcattttg catggtttta gtaatttttt     3540

aataacataa agaaggtctg acagattatg ctaaagagct attgtggtat ggattagaaa     3600

tggcccccac aggctcctgt gttcaaacat cagctagcag tgctgctttg ggttttggaa     3660

cctttaagag gtggggcctt gcttaaggag ataggtcact ggaggtgaac cagacttgct     3720

tccagtctcc ctctctgctg tcccaggaag ccatcatatg aggagtttca caacatactt     3780

ctgctgccac agagtccctc cagtaaggca cgtactggcc actgtgtagt gtaccctcta     3840

aaactgagct agagctcccc tctcctccct acactatctc tgttgatgct ttgtcacggt     3900

gatgaagaac ataagtaagg caaagacaag acatagtttg gagactcacg tgagcatctc     3960

agccagactc aggcacagct gcgatgtggg aattatcaag catgagatgc aaagcaatgg     4020

aaaatgaatc gttatgacag aagcctacat ctagtcttcc cttcttccca ttagtaataa     4080

tagcccgtgt tttagaagaa cacatctttt tggtgttcta ggtagcttat attgcaaatg     4140

tggcacaatc taagagaaat ctgggatgag ggaacctcag tgaaagattc tccttgatca     4200

gattgtccca ctgatgattg atatgggaag gcccaaccca ctgtgaaagg caccacccat     4260

tggcaggatg actggggtta tagaagcaaa caggctgagc atgagccagt gagcaagcca     4320

gtaagcagtg gcttctcttt ggaagaagca tggctttctt ccgtggcttc tgtttgatct     4380

ctctcaatga tagattgtga cctagaagta taagctaaaa taaaccattt cttacccata     4440


<210>  11
<211>  272
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  encoded protein sequence of the engineered mouse IL2RA after humanization

<400>  11

Met Glu Pro Arg Leu Leu Met Leu Gly Phe Leu Ser Leu Thr Ile Val 
1               5                   10                  15      


Pro Ser Cys Arg Ala Glu Leu Cys Asp Asp Asp Pro Pro Glu Ile Pro 
            20                  25                  30          


His Ala Thr Phe Lys Ala Met Ala Tyr Lys Glu Gly Thr Met Leu Asn 
        35                  40                  45              


Cys Glu Cys Lys Arg Gly Phe Arg Arg Ile Lys Ser Gly Ser Leu Tyr 
    50                  55                  60                  


Met Leu Cys Thr Gly Asn Ser Ser His Ser Ser Trp Asp Asn Gln Cys 
65                  70                  75                  80  


Gln Cys Thr Ser Ser Ala Thr Arg Asn Thr Thr Lys Gln Val Thr Pro 
                85                  90                  95      


Gln Pro Glu Glu Gln Lys Glu Arg Lys Thr Thr Glu Met Gln Ser Pro 
            100                 105                 110         


Met Gln Pro Val Asp Gln Ala Ser Leu Pro Gly His Cys Arg Glu Pro 
        115                 120                 125             


Pro Pro Trp Glu Asn Glu Ala Thr Glu Arg Ile Tyr His Phe Val Val 
    130                 135                 140                 


Gly Gln Met Val Tyr Tyr Gln Cys Val Gln Gly Tyr Arg Ala Leu His 
145                 150                 155                 160 


Arg Gly Pro Ala Glu Ser Val Cys Lys Met Thr His Gly Lys Thr Arg 
                165                 170                 175     


Trp Thr Gln Pro Gln Leu Ile Cys Thr Gly Glu Met Glu Thr Ser Gln 
            180                 185                 190         


Phe Pro Gly Glu Glu Lys Pro Gln Ala Ser Pro Glu Gly Arg Pro Glu 
        195                 200                 205             


Ser Glu Thr Ser Cys Leu Val Thr Thr Thr Asp Phe Gln Ile Gln Thr 
    210                 215                 220                 


Glu Met Ala Ala Thr Met Glu Thr Ser Ile Phe Thr Thr Glu Tyr Lys 
225                 230                 235                 240 


Val Ala Val Ala Ser Cys Leu Phe Leu Leu Ile Ser Ile Leu Leu Leu 
                245                 250                 255     


Ser Gly Leu Thr Trp Gln His Arg Trp Arg Lys Ser Arg Arg Thr Ile 
            260                 265                 270         


<210>  12
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The connection between the 5' end of the Neo cassette and the mouse IL2RA gene

<400>  12
ggcagaggcc agcctggtct gcatagtgaa ttccagtata gtcgacggta tcgataagct       60

tgatatcgaa ttccgaagtt                                                   80


<210>  13
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The connection between the 3 end of the Neo cassette with the mouse IL2RA sequence

<400>  13
ttcatcagtc aggtacataa tggtggatcc actagttcta gagcggccgc gccagaacga       60

catagtgaga ccttgtttca                                                   80


<210>  14
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  14
tctggtggaa tttttggggt tcact                                             25


<210>  15
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
tctgtctcac tctttgctgc agttct                                            26


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  16
gctcgactag agcttgcgga                                                   20


<210>  17
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  17
ctgagtgggt ctgagcaaga tgtcc                                             25


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5 probe

<400>  18
gatattccgg tgtgccattc tgcct                                             25


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5 probe

<400>  19
gtagtaggca gcgtcaactc gaagg                                             25


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3 probe

<400>  20
gaagagcaga agaaccatct agcaag                                            26


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3 probe

<400>  21
cattagtgct gagttttact tgggc                                             25


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neo probe

<400>  22
ggatcggcca ttgaacaaga tgg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neo probe

<400>  23
cagaagaact cgtcaagaag gcg                                               23


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  24
actgatcccg aggaggtaga gtacg                                             25


<210>  25
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  25
agggtcacac ttacagttgc tggtg                                             25


<210>  26
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
ctgggctctg tctcactctt tgctg                                             25


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  27
catgggtcag ctgtagtacg ttggg                                             25


<210>  28
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
aggctaaatg ggtagggaga ctgct                                             25


<210>  29
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
gacaagcgtt agtaggcaca tatac                                             25


<210>  30
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
gctccaattt cccacaacat tagt                                              24

