                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
       The Brigham and Women's Hospital, Inc.
       Riella, Leonardo
       Collins, James
       Kaminski, Michael
 
<120>  TRANSPLANT DIAGNOSTICS USING CRISPR-BASED TECHNOLOGY

<130>  M0656.70500WO00

<140>  Not Yet Assigned
<141>  Concurrently Herewith

<150>  US 62/882,652
<151>  2019-08-05

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  1
gaaattaata cgactcacta taggcattgc agagtttctt cagttaggtc taagcc           56


<210>  2
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2
aatttttaag aaaagagccc ttggtttgga ta                                     32


<210>  3
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3
gaaattaata cgactcacta tagggcacca gccgaacgtg gtgatccgcc gatcgatgac       60


<210>  4
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  4
ctatcagcaa ctggaccatg gccagaaaaa tcg                                    33


<210>  5
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
gaaattaata cgactcacta taggtatcca cctacaatcc ttgaaagacc ttaaac           56


<210>  6
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
ttagacatgt ttgaactcca ttcttcagtg ta                                     32


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
cttttcctct tgggcatcat ct                                                22


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
aggaacagcg accctttctc a                                                 21


<210>  9
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  A is modified with fluorescein

<220>
<221>  modified_base
<222>  (28)..(28)
<223>  A is modified with TAMRA

<400>  9
tactggggtt ccttgcactc caatcaga                                          28


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10
gcccgaagcg tttactttga                                                   20


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11
tccattattc ctagctgcgg tatc                                              24


<210>  12
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  A is modified with fluorescein

<220>
<221>  modified_base
<222>  (19)..(19)
<223>  C is modified with TAMRA

<400>  12
aaagcaggcc cgagccgcc                                                    19


<210>  13
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13
gaaattaata cgactcacta tagg                                              24


<210>  14
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14
ctgtgtgaag cagtcaatgc agtagcaagt tttagtcccc ttcgtttttg gggtagtcta       60

aatccctata gtgagtcgta ttaatttc                                          88


<210>  15
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15
cgcgtcagcg gatccacacg gacctcgtgt tttagtcccc ttcgtttttg gggtagtcta       60

aatccctata gtgagtcgta ttaatttc                                          88


<210>  16
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16
gcccttcctg cgagaaaatt gaaatcatgt tttagtcccc ttcgtttttg gggtagtcta       60

aatccctata gtgagtcgta ttaatttc                                          88


<210>  17
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17
gatttagact accccaaaaa cgaaggggac taaaacttgc tactgcattg actgcttcac       60

acag                                                                    64


<210>  18
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18
gatttagact accccaaaaa cgaaggggac taaaacacga ggtccgtgtg gatccgctga       60

cgcg                                                                    64


<210>  19
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  19
gatttagact accccaaaaa cgaaggggac taaaacatga tttcaatttt ctcgcaggaa       60

gggc                                                                    64


<210>  20
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  20
ttgctactgc attgactgct tcacacag                                          28


<210>  21
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  21
acgaggtccg tgtggatccg ctgacgcg                                          28


<210>  22
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  22
atgatttcaa ttttctcgca ggaagggc                                          28


<210>  23
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  23
gaaattaata cgactcacta taggatgaag aaaagtggtg ttcttttcct cttgggcatc       60

atcttgctgg ttctgattgg agtgcaagga accccagtag tgagaaaggg tcgctgttcc      120

tgcatcagca ccaaccaagg gactatccac ctacaatcct tgaaagacct taaacaattt      180

gccccaagcc cttcctgcga gaaaattgaa atcattgcta cactgaagaa tggagttcaa      240

acatgtctaa acccagattc agcagatgtg aaggaactga ttaaaaagtg ggagaaacag      300

gtcagccaaa agaaaaagca aaagaatggg aaaaaacatc aaaaaaagaa agttctgaaa      360

gttcgaaaat ctcaacgttc tcgtcaaaag aagactacat aa                         402


<210>  24
<211>  14
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  A is modified with fluorescein

<220>
<221>  modified_base
<222>  (1)..(1)
<223>  A is 2'-O-methyl A

<220>
<221>  modified_base
<222>  (2)..(2)
<223>  A is riboA

<220>
<221>  modified_base
<222>  (3)..(3)
<223>  U is riboU

<220>
<221>  modified_base
<222>  (4)..(5)
<223>  G is riboG

<220>
<221>  modified_base
<222>  (6)..(6)
<223>  C is riboC

<220>
<221>  modified_base
<222>  (7)..(8)
<223>  A is 2'-O-methyl A

<220>
<221>  modified_base
<222>  (9)..(9)
<223>  A is riboA

<220>
<221>  modified_base
<222>  (10)..(10)
<223>  U is riboU

<220>
<221>  modified_base
<222>  (11)..(12)
<223>  G is riboG

<220>
<221>  modified_base
<222>  (13)..(13)
<223>  C is riboC

<220>
<221>  modified_base
<222>  (14)..(14)
<223>  A is modified with biotin

<220>
<221>  modified_base
<222>  (14)..(14)
<223>  A is 2'-O-methyl A

<400>  24
aauggcaaau ggca                                                         14


<210>  25
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  25
cattgcagag tttcttcagt taggtctaag cc                                     32


<210>  26
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  26
gcaccagccg aacgtggtga tccgccgatc gatgac                                 36


<210>  27
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  27
tatccaccta caatccttga aagaccttaa ac                                     32


<210>  28
<211>  378
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  28
atgaagaaaa gtggtgttct tttcctcttg ggcatcatct tgctggttct gattggagtg       60

caaggaaccc cagtagtgag aaagggtcgc tgttcctgca tcagcaccaa ccaagggact      120

atccacctac aatccttgaa agaccttaaa caatttgccc caagcccttc ctgcgagaaa      180

attgaaatca ttgctacact gaagaatgga gttcaaacat gtctaaaccc agattcagca      240

gatgtgaagg aactgattaa aaagtgggag aaacaggtca gccaaaagaa aaagcaaaag      300

aatgggaaaa aacatcaaaa aaagaaagtt ctgaaagttc gaaaatctca acgttctcgt      360

caaaagaaga ctacataa                                                    378


<210>  29
<211>  1182
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  29

Met Tyr Met Lys Ile Thr Lys Ile Asp Gly Val Ser His Tyr Lys Lys 
1               5                   10                  15      


Gln Asp Lys Gly Ile Leu Lys Lys Lys Trp Lys Asp Leu Asp Glu Arg 
            20                  25                  30          


Lys Gln Arg Glu Lys Ile Glu Ala Arg Tyr Asn Lys Gln Ile Glu Ser 
        35                  40                  45              


Lys Ile Tyr Lys Glu Phe Phe Arg Leu Lys Asn Lys Lys Arg Ile Glu 
    50                  55                  60                  


Lys Glu Glu Asp Gln Asn Ile Lys Ser Leu Tyr Phe Phe Ile Lys Glu 
65                  70                  75                  80  


Leu Tyr Leu Asn Glu Lys Asn Glu Glu Trp Glu Leu Lys Asn Ile Asn 
                85                  90                  95      


Leu Glu Ile Leu Asp Asp Lys Glu Arg Val Ile Lys Gly Tyr Lys Phe 
            100                 105                 110         


Lys Glu Asp Val Tyr Phe Phe Lys Glu Gly Tyr Lys Glu Tyr Tyr Leu 
        115                 120                 125             


Arg Ile Leu Phe Asn Asn Leu Ile Glu Lys Val Gln Asn Glu Asn Arg 
    130                 135                 140                 


Glu Lys Val Arg Lys Asn Lys Glu Phe Leu Asp Leu Lys Glu Ile Phe 
145                 150                 155                 160 


Lys Lys Tyr Lys Asn Arg Lys Ile Asp Leu Leu Leu Lys Ser Ile Asn 
                165                 170                 175     


Asn Asn Lys Ile Asn Leu Glu Tyr Lys Lys Glu Asn Val Asn Glu Glu 
            180                 185                 190         


Ile Tyr Gly Ile Asn Pro Thr Asn Asp Arg Glu Met Thr Phe Tyr Glu 
        195                 200                 205             


Leu Leu Lys Glu Ile Ile Glu Lys Lys Asp Glu Gln Lys Ser Ile Leu 
    210                 215                 220                 


Glu Glu Lys Leu Asp Asn Phe Asp Ile Thr Asn Phe Leu Glu Asn Ile 
225                 230                 235                 240 


Glu Lys Ile Phe Asn Glu Glu Thr Glu Ile Asn Ile Ile Lys Gly Lys 
                245                 250                 255     


Val Leu Asn Glu Leu Arg Glu Tyr Ile Lys Glu Lys Glu Glu Asn Asn 
            260                 265                 270         


Ser Asp Asn Lys Leu Lys Gln Ile Tyr Asn Leu Glu Leu Lys Lys Tyr 
        275                 280                 285             


Ile Glu Asn Asn Phe Ser Tyr Lys Lys Gln Lys Ser Lys Ser Lys Asn 
    290                 295                 300                 


Gly Lys Asn Asp Tyr Leu Tyr Leu Asn Phe Leu Lys Lys Ile Met Phe 
305                 310                 315                 320 


Ile Glu Glu Val Asp Glu Lys Lys Glu Ile Asn Lys Glu Lys Phe Lys 
                325                 330                 335     


Asn Lys Ile Asn Ser Asn Phe Lys Asn Leu Phe Val Gln His Ile Leu 
            340                 345                 350         


Asp Tyr Gly Lys Leu Leu Tyr Tyr Lys Glu Asn Asp Glu Tyr Ile Lys 
        355                 360                 365             


Asn Thr Gly Gln Leu Glu Thr Lys Asp Leu Glu Tyr Ile Lys Thr Lys 
    370                 375                 380                 


Glu Thr Leu Ile Arg Lys Met Ala Val Leu Val Ser Phe Ala Ala Asn 
385                 390                 395                 400 


Ser Tyr Tyr Asn Leu Phe Gly Arg Val Ser Gly Asp Ile Leu Gly Thr 
                405                 410                 415     


Glu Val Val Lys Ser Ser Lys Thr Asn Val Ile Lys Val Gly Ser His 
            420                 425                 430         


Ile Phe Lys Glu Lys Met Leu Asn Tyr Phe Phe Asp Phe Glu Ile Phe 
        435                 440                 445             


Asp Ala Asn Lys Ile Val Glu Ile Leu Glu Ser Ile Ser Tyr Ser Ile 
    450                 455                 460                 


Tyr Asn Val Arg Asn Gly Val Gly His Phe Asn Lys Leu Ile Leu Gly 
465                 470                 475                 480 


Lys Tyr Lys Lys Lys Asp Ile Asn Thr Asn Lys Arg Ile Glu Glu Asp 
                485                 490                 495     


Leu Asn Asn Asn Glu Glu Ile Lys Gly Tyr Phe Ile Lys Lys Arg Gly 
            500                 505                 510         


Glu Ile Glu Arg Lys Val Lys Glu Lys Phe Leu Ser Asn Asn Leu Gln 
        515                 520                 525             


Tyr Tyr Tyr Ser Lys Glu Lys Ile Glu Asn Tyr Phe Glu Val Tyr Glu 
    530                 535                 540                 


Phe Glu Ile Leu Lys Arg Lys Ile Pro Phe Ala Pro Asn Phe Lys Arg 
545                 550                 555                 560 


Ile Ile Lys Lys Gly Glu Asp Leu Phe Asn Asn Lys Asn Asn Lys Lys 
                565                 570                 575     


Tyr Glu Tyr Phe Lys Asn Phe Asp Lys Asn Ser Ala Glu Glu Lys Lys 
            580                 585                 590         


Glu Phe Leu Lys Thr Arg Asn Phe Leu Leu Lys Glu Leu Tyr Tyr Asn 
        595                 600                 605             


Asn Phe Tyr Lys Glu Phe Leu Ser Lys Lys Glu Glu Phe Glu Lys Ile 
    610                 615                 620                 


Val Leu Glu Val Lys Glu Glu Lys Lys Ser Arg Gly Asn Ile Asn Asn 
625                 630                 635                 640 


Lys Lys Ser Gly Val Ser Phe Gln Ser Ile Asp Asp Tyr Asp Thr Lys 
                645                 650                 655     


Ile Asn Ile Ser Asp Tyr Ile Ala Ser Ile His Lys Lys Glu Met Glu 
            660                 665                 670         


Arg Val Glu Lys Tyr Asn Glu Glu Lys Gln Lys Asp Thr Ala Lys Tyr 
        675                 680                 685             


Ile Arg Asp Phe Val Glu Glu Ile Phe Leu Thr Gly Phe Ile Asn Tyr 
    690                 695                 700                 


Leu Glu Lys Asp Lys Arg Leu His Phe Leu Lys Glu Glu Phe Ser Ile 
705                 710                 715                 720 


Leu Cys Asn Asn Asn Asn Asn Val Val Asp Phe Asn Ile Asn Ile Asn 
                725                 730                 735     


Glu Glu Lys Ile Lys Glu Phe Leu Lys Glu Asn Asp Ser Lys Thr Leu 
            740                 745                 750         


Asn Leu Tyr Leu Phe Phe Asn Met Ile Asp Ser Lys Arg Ile Ser Glu 
        755                 760                 765             


Phe Arg Asn Glu Leu Val Lys Tyr Lys Gln Phe Thr Lys Lys Arg Leu 
    770                 775                 780                 


Asp Glu Glu Lys Glu Phe Leu Gly Ile Lys Ile Glu Leu Tyr Glu Thr 
785                 790                 795                 800 


Leu Ile Glu Phe Val Ile Leu Thr Arg Glu Lys Leu Asp Thr Lys Lys 
                805                 810                 815     


Ser Glu Glu Ile Asp Ala Trp Leu Val Asp Lys Leu Tyr Val Lys Asp 
            820                 825                 830         


Ser Asn Glu Tyr Lys Glu Tyr Glu Glu Ile Leu Lys Leu Phe Val Asp 
        835                 840                 845             


Glu Lys Ile Leu Ser Ser Lys Glu Ala Pro Tyr Tyr Ala Thr Asp Asn 
    850                 855                 860                 


Lys Thr Pro Ile Leu Leu Ser Asn Phe Glu Lys Thr Arg Lys Tyr Gly 
865                 870                 875                 880 


Thr Gln Ser Phe Leu Ser Glu Ile Gln Ser Asn Tyr Lys Tyr Ser Lys 
                885                 890                 895     


Val Glu Lys Glu Asn Ile Glu Asp Tyr Asn Lys Lys Glu Glu Ile Glu 
            900                 905                 910         


Gln Lys Lys Lys Ser Asn Ile Glu Lys Leu Gln Asp Leu Lys Val Glu 
        915                 920                 925             


Leu His Lys Lys Trp Glu Gln Asn Lys Ile Thr Glu Lys Glu Ile Glu 
    930                 935                 940                 


Lys Tyr Asn Asn Thr Thr Arg Lys Ile Asn Glu Tyr Asn Tyr Leu Lys 
945                 950                 955                 960 


Asn Lys Glu Glu Leu Gln Asn Val Tyr Leu Leu His Glu Met Leu Ser 
                965                 970                 975     


Asp Leu Leu Ala Arg Asn Val Ala Phe Phe Asn Lys Trp Glu Arg Asp 
            980                 985                 990         


Phe Lys Phe Ile Val Ile Ala Ile  Lys Gln Phe Leu Arg  Glu Asn Asp 
        995                 1000                 1005             


Lys Glu  Lys Val Asn Glu Phe  Leu Asn Pro Pro Asp  Asn Ser Lys 
    1010                 1015                 1020             


Gly Lys  Lys Val Tyr Phe Ser  Val Ser Lys Tyr Lys  Asn Thr Val 
    1025                 1030                 1035             


Glu Asn  Ile Asp Gly Ile His  Lys Asn Phe Met Asn  Leu Ile Phe 
    1040                 1045                 1050             


Leu Asn  Asn Lys Phe Met Asn  Arg Lys Ile Asp Lys  Met Asn Cys 
    1055                 1060                 1065             


Ala Ile  Trp Val Tyr Phe Arg  Asn Tyr Ile Ala His  Phe Leu His 
    1070                 1075                 1080             


Leu His  Thr Lys Asn Glu Lys  Ile Ser Leu Ile Ser  Gln Met Asn 
    1085                 1090                 1095             


Leu Leu  Ile Lys Leu Phe Ser  Tyr Asp Lys Lys Val  Gln Asn His 
    1100                 1105                 1110             


Ile Leu  Lys Ser Thr Lys Thr  Leu Leu Glu Lys Tyr  Asn Ile Gln 
    1115                 1120                 1125             


Ile Asn  Phe Glu Ile Ser Asn  Asp Lys Asn Glu Val  Phe Lys Tyr 
    1130                 1135                 1140             


Lys Ile  Lys Asn Arg Leu Tyr  Ser Lys Lys Gly Lys  Met Leu Gly 
    1145                 1150                 1155             


Lys Asn  Asn Lys Phe Glu Ile  Leu Glu Asn Glu Phe  Leu Glu Asn 
    1160                 1165                 1170             


Val Lys  Ala Met Leu Glu Tyr  Ser Glu 
    1175                 1180         


