                         SEQUENCE LISTING

<110>  University of Florida Research Foundation, Incorporated
 
<120>  RNA GUIDE GENOME EDITING IN CITRUS USING CRISPR-RIBONUCLEOPROTEIN
        COMPLEXES

<130>  10457-434PC0

<140>  pct/us20/44766
<141>  2020-08-03

<150>  62/882375
<151>  2019-08-02

<160>  163   

<170>  PatentIn version 3.5

<210>  1
<211>  4107
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimzed Cas sequence

<400>  1
atggacaaga agtacagcat cggtcttgat attggtacta actctgtggg ttgggctgtt       60

attactgatg agtataaggt gcctagcaag aagtttaagg tgcttggtaa taccgacaga      120

cattctatta agaagaatct tattggtgct cttctttttg attctggcga gactgctgag      180

gctactagac ttaagagaac tgctagaaga aggtacacta gaagaaagaa caggatctgc      240

taccttcaag agattttctc taatgagatg gccaaggttg acgattcttt ttttcacaga      300

ctcgaggagt ctttccttgt tgaggaggat aagaagcatg agagacatcc tattttcggt      360

aacatcgttg acgaggttgc ttatcatgag aagtatccta ctatctacca ccttagaaag      420

aagcttgtgg attctactga caaggctgat cttagactta tctaccttgc tcttgctcat      480

atgatcaagt tcagaggtca tttcctcatc gagggtgatc ttaatcctga taatagcgat      540

gtggacaagc tttttatcca gcttgttcaa acctacaacc agctttttga ggagaatcct      600

atcaacgctt ctggtgttga tgctaaggct attctttctg ctagactttc taagagcagg      660

agacttgaga atcttatcgc tcaacttcct ggtgagaaga agaatggtct ttttggtaac      720

ctcatcgctc tttctcttgg tcttactcct aatttcaaga gcaacttcga ccttgctgag      780

gatgctaagc ttcaactttc taaggatacc tacgacgatg atcttgataa ccttcttgct      840

caaatcggtg atcaatacgc tgatcttttc cttgctgcta agaatcttag cgatgctatt      900

cttctcagcg atattcttag ggttaacact gagatcacca aggctcctct ttctgcttct      960

atgattaaga gatacgacga gcatcaccaa gatcttactc ttcttaaggc tcttgtgaga     1020

caacaacttc ctgagaagta taaggagatc ttcttcgatc agagcaagaa tggttacgct     1080

ggttatattg acggtggtgc ttctcaagag gagttttata agttcatcaa gcccatcctc     1140

gagaagatgg atggtactga ggagcttctt gttaagctta atagggagga tctccttaga     1200

aagcaaagaa ctttcgacaa cggttctatc cctcatcaaa ttcatctcgg tgagcttcat     1260

gctattctta gaagacaaga ggacttctac ccttttctta aggataacag ggagaagatc     1320

gagaagattc ttactttcag gattccttac tacgtgggtc ctcttgctag aggtaattct     1380

agatttgctt ggatgaccag aaagtctgag gagactatta ctccttggaa tttcgaggag     1440

gttgttgata agggtgcttc tgctcaatct tttatcgaga gaatgaccaa cttcgacaag     1500

aatcttccta acgagaaggt tcttcctaag cattctcttc tttacgagta cttcaccgtt     1560

tacaacgagc ttactaaggt taagtacgtg actgagggta tgagaaagcc tgcttttctt     1620

tctggtgagc aaaagaaggc tatcgttgat cttcttttca agaccaacag gaaggttact     1680

gttaagcaac ttaaggagga ctacttcaag aagatcgagt gctttgatag cgttgagatt     1740

tctggtgttg aggatagatt caacgcttct cttggtactt accatgatct tcttaagatc     1800

atcaaggaca aggacttcct tgataacgag gagaatgagg atatccttga ggatattgtg     1860

ctcactctta ctcttttcga ggatagagag atgatcgagg agagacttaa gacttatgct     1920

catcttttcg acgacaaggt tatgaagcaa cttaagagaa ggaggtacac tggttggggt     1980

agactttcta gaaagcttat taacggcatc agggataagc aatctggtaa gactatcctt     2040

gacttcctta agtctgacgg ttttgctaat aggaacttca tgcaactcat ccatgacgat     2100

tctcttactt tcaaggagga tatccagaag gctcaagttt ctggtcaagg tgattctctt     2160

catgagcata ttgctaacct cgctggttct cctgctatta agaagggtat tcttcagact     2220

gtgaaggttg ttgatgagct tgttaaggtt atgggtagac ataagcctga gaatatcgtt     2280

atcgagatgg ctagagagaa ccaaactact caaaagggtc aaaagaacag cagagagaga     2340

atgaagagaa tcgaggaggg tattaaggag cttggttctc aaattctcaa ggagcatcct     2400

gttgagaata ctcaacttca aaacgagaag ctctaccttt actaccttca aaacggtaga     2460

gacatgtacg ttgatcaaga gcttgatatc aacaggcttt ctgattacga cgttgatcat     2520

atcgtgcctc aatcttttct caaggatgat tctatcgaca acaaggttct cactagatct     2580

gataagaaca ggggtaagtc tgataacgtt ccttctgagg aggttgttaa gaagatgaag     2640

aactactgga ggcaacttct taacgctaag cttattaccc agagaaagtt cgataacctc     2700

actaaggctg agagaggtgg tctttctgag cttgataagg ctggttttat caagagacag     2760

cttgttgaga ctaggcaaat tactaagcac gttgctcaaa tcctcgattc tagaatgaac     2820

actaagtacg acgagaacga taagcttatc agagaggtta aggtgatcac tcttaagtct     2880

aagctcgttt ctgacttcag aaaggatttc caattctaca aggtgaggga gattaacaac     2940

taccatcatg ctcatgacgc ttatcttaac gctgttgttg gtactgctct tattaagaag     3000

taccctaagc tcgagtctga gtttgtttat ggtgattaca aggtgtacga cgttagaaag     3060

atgatcgcta agtctgagca agagattggt aaggctactg ctaagtattt cttctacagc     3120

aacatcatga acttcttcaa gaccgagatc actcttgcta atggtgagat tagaaagagg     3180

cctcttatcg agactaacgg tgagactggt gagattgttt gggataaggg tagagatttc     3240

gctactgtta gaaaggttct ctctatgcct caagttaaca tcgttaagaa gaccgaggtt     3300

caaactggtg gtttttctaa ggagtctatc cttcctaaga ggaatagcga taagcttatc     3360

gctagaaaga aggattggga ccctaagaag tatggtggtt ttgattctcc taccgttgct     3420

tattctgtgc ttgttgttgc taaggttgag aagggtaagt ctaagaagct taagtctgtg     3480

aaggagcttc ttggtattac tatcatggag agaagctctt tcgagaagaa tcctattgac     3540

ttcctcgagg ctaagggtta taaggaggtt aagaaggatc tcatcatcaa gcttcctaag     3600

tactctcttt tcgagcttga gaatggtaga aagagaatgc ttgcttctgc tggtgagctt     3660

caaaagggta atgagcttgc tcttccttct aagtatgtga atttcctcta cctcgcttct     3720

cattatgaga agcttaaggg ttctcctgag gataatgagc aaaagcaact tttcgtggag     3780

caacataagc attaccttga tgagatcatc gagcaaatct ctgagttttc taagagggtt     3840

atcctcgctg atgctaatct tgataaggtt cttagcgctt acaacaagca tagagataag     3900

cctatcaggg agcaagctga gaatattatt caccttttca ccctcaccaa ccttggtgct     3960

cctgctgctt ttaagtattt tgataccacc atcgacagga agagatatac ttctactaag     4020

gaggtgctcg atgctactct tattcatcaa tctatcaccg gtctctacga gactagaatt     4080

gatctttctc agctcggtgg tgattaa                                         4107


<210>  2
<211>  3794
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  optimized Cas12a sequence

<400>  2
atggccccca agaagaagag aaaggttggc attcacggcg ttcccgcagc aagcaagtta       60

gagaagttta cgaattgtta ctcactgtca aagacgctgc gttttaaggc tatccctgtc      120

gggaagacgc aagagaatat cgacaataag cgactgttag tcgaggacga gaagcgcgcc      180

gaggactaca agggcgtcaa gaagctgtta gaccgctatt acctgagctt tatcaatgac      240

gtcttacact caatcaagct gaagaatctg aataactaca tctcactgtt tcgtaagaag      300

acgagaacgg agaaggagaa taaggagctg gagaatctgg agatcaatct gcgtaaggag      360

atcgcgaagg cttttaaggg aaatgagggg tacaagtcac tgtttaagaa ggacatcatc      420

gagacgatcc tgcctgagtt tttagacgac aaggacgaga tcgccctggt caatagcttt      480

aatggcttta cgacggcgtt tacgggcttt ttcgacaatc gggagaatat gttttcagag      540

gaagcgaagt caacgtcaat cgcttttcgt tgcatcaatg agaatctgac gcggtatatc      600

tcaaatatgg acatctttga gaaggtcgac gctatctttg acaagcacga ggtccaagag      660

atcaaggaga agatcctgaa ttcagactac gacgtcgagg actttttcga gggagagttt      720

ttcaattttg tcttaacgca agaggggatc gacgtctata atgccatcat cggcggtttt      780

gtcacggagt caggagagaa gatcaagggg ctgaatgagt atatcaatct gtacaatcaa      840

aagacgaagc aaaagctgcc taagtttaag cctctgtaca agcaagtctt atcagacagg      900

gagtcattat cattttatgg cgagggttac acgtcagacg aggaagtcct ggaggtcttt      960

agaaatacgc tgaataagaa ttcagagatc ttttcatcaa tcaagaagct ggagaagctg     1020

tttaagaatt ttgacgagta ttcatcagct ggaatctttg tcaagaatgg gcctgccatc     1080

tcaacgatct caaaggacat ctttggcgag tggaatgtca tccgtgacaa gtggaatgct     1140

gagtacgacg acatccacct gaagaagaag gccgtcgtca cggagaagta tgaggacgac     1200

cgtcgtaaga gctttaagaa gatcgggtca ttttcactgg agcaattaca agagtacgcc     1260

gacgcggacc tgtcagtcgt cgagaagtta aaggagatca tcatccaaaa ggtcgacgag     1320

atctataagg tctacggctc atcagagaag ttatttgacg ccgactttgt cctggagaag     1380

tcattaaaga agaatgacgc ggtcgtcgct atcatgaagg acctgttaga ctcagtcaag     1440

tcatttgaga attatatcaa ggcgtttttc ggagagggga aggagacgaa tagggacgag     1500

tcattttacg gtgactttgt cctggcctat gacatcctgt taaaggtcga ccacatctat     1560

gacgcgatca gaaattacgt cacgcaaaag ccttactcaa aggacaagtt taagctgtac     1620

tttcaaaatc ctcagtttat gggcggctgg gacaaggaca aggagacgga ctaccgtgcg     1680

acgatcctgc ggtatggatc aaagtattac ttagccatca tggacaagaa gtatgcgaag     1740

tgcctgcaaa agatcgacaa ggacgacgtc aatggcaatt acgagaagat caattacaag     1800

ctgctgcctg gtcctaataa gatgttacct aaggtctttt tctcaaagaa gtggatggct     1860

tattacaatc cttcagagga catccaaaag atctataaga atggcacgtt taagaagggt     1920

gacatgttta atctgaatga ctgtcacaag ttaatcgact ttttcaagga ctcaatctca     1980

cgatacccta agtggtcaaa tgcttacgac tttaattttt cagagacgga gaagtacaag     2040

gacatcgccg gcttttatcg cgaggtcgag gagcaaggtt ataaggtcag ctttgagtca     2100

gcgtcaaaga aggaagtcga caagctggtc gaggaaggca agctgtacat gtttcaaatc     2160

tacaataagg acttttcaga caagtcacac gggacgccta atctgcacac gatgtacttt     2220

aagctgctgt ttgacgagaa taaccacggg caaatcaggc tgtcaggcgg tgctgagctg     2280

tttatgagga gagcctcact gaagaaggaa gagttagtcg tccaccctgc taattcacct     2340

atcgccaata agaatcccga caatcctaag aagacgacga cgctgtcata tgacgtctac     2400

aaggacaagc gtttttcaga ggaccaatat gagttacaca tccctatcgc gatcaataag     2460

tgtcctaaga atatctttaa gatcaatacg gaggtccggg tcctgttaaa gcacgacgac     2520

aatccttacg tcatcggcat cgaccgtggt gagcggaatc tgttatatat cgtcgtcgtc     2580

gacggcaagg gtaatatcgt cgagcaatac tcactgaatg agatcatcaa taactttaat     2640

ggtatcagaa tcaagacgga ctatcactca ctgttagaca agaaggagaa ggagcgattt     2700

gaggcgcgcc aaaattggac gtcaatcgag aatatcaagg agctgaaggc tggttacatc     2760

tcacaagtcg tccacaagat ctgcgagtta gtcgagaagt atgacgccgt catcgcgctg     2820

gaggacttaa attcaggctt taagaattca cgagtcaagg tcgagaagca agtctaccaa     2880

aagtttgaga agatgctgat cgacaagctg aattacatgg tcgacaagaa gtcaaatcct     2940

tgtgctacgg gaggggccct gaagggctac caaatcacga ataagtttga gagctttaag     3000

tcaatgtcaa cgcaaaatgg ttttatcttt tatatccctg cgtggttaac gtcaaagatc     3060

gacccttcaa cgggatttgt caatctgtta aagacgaagt acacgtcaat cgctgactca     3120

aagaagttta tctcatcatt tgaccgaatc atgtatgtcc ctgaggaaga cctgtttgag     3180

tttgcgttag actacaagaa tttttcacgc acggacgctg actatatcaa gaagtggaag     3240

ctgtattcat acggcaatcg aatccgcatc tttaggaatc ctaagaagaa taacgtcttt     3300

gactgggagg aagtctgcct gacgtcagcc tacaaggagc tgtttaataa gtacggcatc     3360

aattaccaac aaggtgacat cagagcgctg ttatgtgagc aatcagacaa ggcgttttat     3420

tcaagcttta tggctctgat gtcactgatg ttacaaatga ggaattcaat cacgggcaga     3480

acggacgtcg actttttaat ctcacctgtc aagaattcag acggtatctt ttatgactca     3540

cgtaattacg aggcgcaaga gaatgctatc ctgcctaaga atgctgacgc caatggcgcc     3600

tacaatatcg cgcggaaggt cttatgggcc atcgggcagt ttaagaaggc ggaggacgag     3660

aagctggaca aggtcaagat cgcgatctca aataaggagt ggttagagta tgctcaaacg     3720

tcagtgaagc acaagcgacc cgcagcaacc aagaaggcag ggcaggcgaa gaagaagaag     3780

taagacgact agtg                                                       3794


<210>  3
<211>  180
<212>  DNA
<213>  Arabidopsis thaliana

<400>  3
caatcttcaa aagtaccaca tcgcttaggt aaagaaagca gctgagttta tatatggtta       60

gagacgaagt agtgattgtc ccttcgggga catccgataa aattggaacg atacagagaa      120

gattagcatg gcccctgcgc aaggatgaca cgcataaatc gagaaatggt ccaaattttt      180


<210>  4
<211>  177
<212>  DNA
<213>  Citrus sinensis

<400>  4
tggaacctaa tcccacatcg cttgtttatg gtaaagtttg agtctttaaa taaaacatgc       60

agggcgaatg ggctgtctct tcggagacat ccgataaaat tggaacgata cagagaagat      120

tagcatggcc cctgcgcaag gatgacacgc acaaatcgag aaatggtcca aattttt         177


<210>  5
<211>  179
<212>  DNA
<213>  Citrus sinensis

<400>  5
ctttactcac agtcccacat cgaaaatatg tattacggag ccgttgttta tatataggag       60

gagagaacga acggccgtct cttcggagac atccgataaa attggaacga tacagagaag      120

attagcatgg cccctgcgca aggatgacac gcacaaatcg agaaatggtc caaattttt       179


<210>  6
<211>  177
<212>  DNA
<213>  Citrus sinensis

<400>  6
ttgaagaaaa tcccacatcg aaagaaaaaa ctgaataaac atggtctata tatacaagga       60

ctcccaggtt ggttgtctct tcggagacat ccgataaaat tggaacgata cagagaagat      120

tagcatggcc cctgcgcaag gatgacacgc acaaatcgag aaatggtcca aattttt         177


<210>  7
<211>  172
<212>  DNA
<213>  Citrus sinensis

<400>  7
ctaaatccca catcgtctag ttgcgcttcg ttgagagtct taatataatc ataaccaacc       60

agtgcgattg tctcttcgga gacatccgat aaaattggaa cgatacagag aagattagca      120

tggcccctgc gcaaggatga cacgcacaaa tcgagaaatg gtccaaattt tt              172


<210>  8
<211>  172
<212>  DNA
<213>  Citrus sinensis

<400>  8
ctaaatccca cattgtttag ttatccttcc ttccgagtct taatatctac aagagccgca       60

tgccctatcg tctctccgga gacatccgat aaaattggaa cgatacagag aagattagca      120

tggcccctgc gcaaggatga cacgcacaaa tcgagaaatg gtccaaattt tt              172


<210>  9
<211>  694
<212>  DNA
<213>  Citrus sinensis

<400>  9
tatgataata aaactcccag aggggtcaca tttatttgag gagaatttaa aaaaaaaaaa       60

atcaccagaa agaggtgccg atgaatgcaa taagggatgc aaattattta tttaccttta      120

caggcattta gtatttaatt ttattttcag ttattgttca ctgataatcg aattttaatg      180

gagtcggtca tggaaggcat gcacataaaa tatgaggatg caggtaaggt ttgcgcggtc      240

tgaaatttca atgacggcgt acctctctct atcacaagaa atatgtaata atatgtttgt      300

aatttagaat ttttttctgt aattgacagt attttgtaga taaggaatgc cactctatag      360

gtactgctaa aagatgggcc aatataggtg cggcccatga gcccattgta tttaaagaat      420

ctttactcac agtcccacat cgaaaatatg tattacggag ccgttgttta tatataggag      480

gagagaacga acggccgtct cttcggagac atccgataaa attggaacga tacagagaag      540

attagcatgg cccctgcgca aggatgacac gcacaaatcg agaaatggtc caaatttttt      600

tcttccccct cattcgggct tgtttaatca gtcggcagtt tgacgatgtt agcttctgca      660

gtttgattcc atcgttaacc atataaattt tttt                                  694


<210>  10
<211>  662
<212>  DNA
<213>  Citrus sinensis

<400>  10
tcaggagccg gttgaatttg attgttgttt gatgtttagg tatgcttaca attttttact       60

aaataaggta ataattgttc cttttatttt attcatttag cttggtactt gtcgtaatct      120

atctccgtca atgtcagctt cttctgtgtc ggaagaagct gaaaaatgtt gttgaaggcc      180

ctttcattta ttattatcat tattatgttt aattttgttc ggcaaatgtg tactaatttg      240

gttcgaatcg ttcgatctca taatacaaac atgtaatgag ttatttatga cgtcatggat      300

gcggttagtt tgcgtggtct gaaatttcaa ccaaattatt caatcatggt ggtggccggc      360

tggtgcctat cccttgattg aaatatgcaa ttaaaattct cgtaataatg ttgatttgtt      420

ctatatactt gaattgatgg tattaattat tgttataaaa gcactaactt gtttgagaaa      480

ggggataact aaaaggtaat aataataata ataaaataaa gaaaaggccc aacacatggg      540

cgccctatct gttgggccaa atgccataag aggatccagc tagcccgttg aagaaaatcc      600

cacatcgaaa gaaaaaactg aataaacatg gtctatatat acaaggactc ccaggttggt      660

tg                                                                     662


<210>  11
<211>  522
<212>  DNA
<213>  Citrus sinensis

<400>  11
tgttgtgcgg gatcttttaa gtaggaactg acaagtttcc ctcacccaca tctttcgtga       60

agcgaattca gctacagatt tcatggctaa tatggctcat tcaatctctc atgggttaca      120

tttatttact agcctacatg tgtgtattta ttcaagttta ttgcaagaca tgtttggggt      180

tgctaaactc cgtattgttc cggtttagct cgctttattg agcccatctt aataaaaaaa      240

aaaaaaagac ttccttctat gattttaggg aaaattacta taatattatc cataagagta      300

aattttggac ctacgcgtgc agatatgtac atatattttt taataaaaac aattactgca      360

tggactggac ttcgtagcat cttttttggg ctcaggaagg gcctaaagtt atgatagcca      420

gacagacgtc atgtggttcg gttcaaattg aactaaatcc cacatcgtct agttgcgctt      480

cgttgagagt cttaatataa tcataaccaa ccagtgcgat tg                         522


<210>  12
<211>  44
<212>  DNA
<213>  Francisella novicida

<400>  12
ttgcagcagc agcagcagca gcagcaacag ttcatggata ctag                        44


<210>  13
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alt-allele 1 FnCPf1

<400>  13
ttgcagcagc agcagcagca gcagcaaggg ttcatggata ctag                        44


<210>  14
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alt-allele 2 FnCpf1

<400>  14
ttgcagcacc agcagcacca gcagcaacag ttcatggata ctag                        44


<210>  15
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alt-allele3 FnCpf1

<400>  15
ttgcatcagc ggcagcagca gcagcaacag ttcatggata ctag                        44


<210>  16
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  16
gucgucgucg ucgucgucgu cguugucuca ucuugaa                                37


<210>  17
<211>  35
<212>  DNA
<213>  Francisella novicida

<400>  17
gtcttgcagc agcagcagca gcagcagcaa cagtt                                  35


<210>  18
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FnCpf1 mutatnt

<400>  18
gtcttgcagc agcagcagca gcagcaacag tt                                     32


<210>  19
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FnCpf1 mutant

<400>  19
gtcttgcagc agcagcagca gcatcagcaa cagtt                                  35


<210>  20
<211>  24
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA

<400>  20
gccgacgcgg ccccgauaaa cggu                                              24


<210>  21
<211>  12
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  extension of crRNA

<400>  21
ucucaucuuu aa                                                           12


<210>  22
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wild type target

<400>  22
cctgtttacg gctgcgccgg ggctatttgc catctccag                              39


<210>  23
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant allele

<400>  23
cctgtttacg gctgcgccgt ggctatttgc catctccag                              39


<210>  24
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wild-type target

<400>  24
tttacggctg cgccggggct atttgccatc tccagaaaca agt                         43


<210>  25
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant target

<400>  25
tttacggctg cgctggggct atttgccatc tccagaaaca agt                         43


<210>  26
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CslOB1 target

<400>  26
tttaaagctt ctccttcaca atcctctcc                                         29


<210>  27
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target site

<400>  27
tggaatctgg ctaaatcagt gtgcttgtgg agggagacat gtagggga                    48


<210>  28
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target site

<400>  28
ggctaaatca gtgtgcttgt ggagggagac tgtaggggaa tc                          42


<210>  29
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant target site

<400>  29
tggaatctgg ctaaatcagt gtgcttgtgg agggagactg tagggga                     47


<210>  30
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target site

<400>  30
ttgtgcacaa gcaattgtac agg                                               23


<210>  31
<211>  9640
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMAL-C5X/AsCpf1 vector

<400>  31
gaattccctg caggtaatta aataagcttc aaataaaacg aaaggctcag tcgaaagact       60

gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc      120

cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc      180

cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt      240

ttctacaaac tctttcggtc cgttgtttat ttttctaaat acattcaaat atgtatccgc      300

tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta      360

ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg      420

ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg      480

gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac      540

gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg      600

acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt      660

actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg      720

ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac      780

cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt      840

gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag      900

caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc      960

aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc     1020

ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta     1080

tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg     1140

ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga     1200

ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttcctta     1260

ggactgagcg tcaaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     1320

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     1380

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     1440

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     1500

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     1560

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     1620

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     1680

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     1740

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     1800

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     1860

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     1920

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     1980

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     2040

cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg     2100

catctgtgcg gtatttcaca ccgcatataa ggtgcactgt gactgggtca tggctgcgcc     2160

ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     2220

ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc     2280

accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca     2340

gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg     2400

gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc     2460

cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct     2520

cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca     2580

actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt     2640

cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg     2700

gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc     2760

gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt     2820

tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg     2880

ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga     2940

aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa     3000

gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat     3060

gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg     3120

aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg     3180

gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc     3240

ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc     3300

agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac     3360

aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat     3420

gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac     3480

cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag     3540

catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc     3600

tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg     3660

atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg     3720

ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc     3780

gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatttc ggtagtggga     3840

tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat     3900

tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg     3960

gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc     4020

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag     4080

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca     4140

ttaggcacaa ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct     4200

ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa     4260

ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac     4320

ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg     4380

tggaattgtg agcggataac aatttcacac aggaaacagc cagtccgttt aggtgttttc     4440

acgagcaatt gaccaacaag gaccatagat tatgaaaatc gaagaaggta aactggtaat     4500

ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa     4560

agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca     4620

ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg     4680

ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct     4740

gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc     4800

tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg     4860

ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt     4920

caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt     4980

caagtatgaa aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa     5040

agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga     5100

ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc     5160

gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac     5220

cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc     5280

cagtccgaac aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg     5340

tctggaagcg gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga     5400

agagttggtg aaagatccgc gtattgccgc cactatggaa aacgcccaga aaggtgaaat     5460

catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa     5520

cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag     5580

ctcgaacaac aacaacaata acaataacaa caacctcggg atcgagggaa ggatttcaca     5640

tatgtccatg ggcggccgcg atatcgtcga catgacacag ttcgagggct ttaccaacct     5700

gtatcaggtg agcaagacac tgcggtttga gctgatccca cagggcaaga ccctgaagca     5760

catccaggag cagggcttca tcgaggagga caaggcccgc aatgatcact acaaggagct     5820

gaagcccatc atcgatcgga tctacaagac ctatgccgac cagtgcctgc agctggtgca     5880

gctggattgg gagaacctga gcgccgccat cgactcctat agaaaggaga aaaccgagga     5940

gacaaggaac gccctgatcg aggagcaggc cacatatcgc aatgccatcc acgactactt     6000

catcggccgg acagacaacc tgaccgatgc catcaataag agacacgccg agatctacaa     6060

gggcctgttc aaggccgagc tgtttaatgg caaggtgctg aagcagctgg gcaccgtgac     6120

cacaaccgag cacgagaacg ccctgctgcg gagcttcgac aagtttacaa cctacttctc     6180

cggcttttat gagaacagga agaacgtgtt cagcgccgag gatatcagca cagccatccc     6240

acaccgcatc gtgcaggaca acttccccaa gtttaaggag aattgtcaca tcttcacacg     6300

cctgatcacc gccgtgccca gcctgcggga gcactttgag aacgtgaaga aggccatcgg     6360

catcttcgtg agcacctcca tcgaggaggt gttttccttc cctttttata accagctgct     6420

gacacagacc cagatcgacc tgtataacca gctgctggga ggaatctctc gggaggcagg     6480

caccgagaag atcaagggcc tgaacgaggt gctgaatctg gccatccaga agaatgatga     6540

gacagcccac atcatcgcct ccctgccaca cagattcatc cccctgttta agcagatcct     6600

gtccgatagg aacaccctgt ctttcatcct ggaggagttt aagagcgacg aggaagtgat     6660

ccagtccttc tgcaagtaca agacactgct gagaaacgag aacgtgctgg agacagccga     6720

ggccctgttt aacgagctga acagcatcga cctgacacac atcttcatca gccacaagaa     6780

gctggagaca atcagcagcg ccctgtgcga ccactgggat acactgagga atgccctgta     6840

tgagcggaga atctccgagc tgacaggcaa gatcaccaag tctgccaagg agaaggtgca     6900

gcgcagcctg aagcacgagg atatcaacct gcaggagatc atctctgccg caggcaagga     6960

gctgagcgag gccttcaagc agaaaaccag cgagatcctg tcccacgcac acgccgccct     7020

ggatcagcca ctgcctacaa ccctgaagaa gcaggaggag aaggagatcc tgaagtctca     7080

gctggacagc ctgctgggcc tgtaccacct gctggactgg tttgccgtgg atgagtccaa     7140

cgaggtggac cccgagttct ctgcccggct gaccggcatc aagctggaga tggagccttc     7200

tctgagcttc tacaacaagg ccagaaatta tgccaccaag aagccctact ccgtggagaa     7260

gttcaagctg aactttcaga tgcctacact ggcctctggc tgggacgtga ataaggagaa     7320

gaacaatggc gccatcctgt ttgtgaagaa cggcctgtac tatctgggca tcatgccaaa     7380

gcagaagggc aggtataagg ccctgagctt cgagcccaca gagaaaacca gcgagggctt     7440

tgataagatg tactatgact acttccctga tgccgccaag atgatcccaa agtgcagcac     7500

ccagctgaag gccgtgacag cccactttca gacccacaca acccccatcc tgctgtccaa     7560

caatttcatc gagcctctgg agatcacaaa ggagatctac gacctgaaca atcctgagaa     7620

ggagccaaag aagtttcaga cagcctacgc caagaaaacc ggcgaccaga agggctacag     7680

agaggccctg tgcaagtgga tcgacttcac aagggatttt ctgtccaagt ataccaagac     7740

aacctctatc gatctgtcta gcctgcggcc atcctctcag tataaggacc tgggcgagta     7800

ctatgccgag ctgaatcccc tgctgtacca catcagcttc cagagaatcg ccgagaagga     7860

gatcatggat gccgtggaga caggcaagct gtacctgttc cagatctata acaaggactt     7920

tgccaagggc caccacggca agcctaatct gcacacactg tattggaccg gcctgttttc     7980

tccagagaac ctggccaaga caagcatcaa gctgaatggc caggccgagc tgttctaccg     8040

ccctaagtcc aggatgaaga ggatggcaca ccggctggga gagaagatgc tgaacaagaa     8100

gctgaaggat cagaaaaccc caatccccga caccctgtac caggagctgt acgactatgt     8160

gaatcacaga ctgtcccacg acctgtctga tgaggccagg gccctgctgc ccaacgtgat     8220

caccaaggag gtgtctcacg agatcatcaa ggataggcgc tttaccagcg acaagttctt     8280

tttccacgtg cctatcacac tgaactatca ggccgccaat tccccatcta agttcaacca     8340

gagggtgaat gcctacctga aggagcaccc cgagacacct atcatcggca tcgatcgggg     8400

cgagagaaac ctgatctata tcacagtgat cgactccacc ggcaagatcc tggagcagcg     8460

gagcctgaac accatccagc agtttgatta ccagaagaag ctggacaaca gggagaagga     8520

gagggtggca gcaaggcagg cctggtctgt ggtgggcaca atcaaggatc tgaagcaggg     8580

ctatctgagc caggtcatcc acgagatcgt ggacctgatg atccactacc aggccgtggt     8640

ggtgctggag aacctgaatt tcggctttaa gagcaagagg accggcatcg ccgagaaggc     8700

cgtgtaccag cagttcgaga agatgctgat cgataagctg aattgcctgg tgctgaagga     8760

ctatccagca gagaaagtgg gaggcgtgct gaacccatac cagctgacag accagttcac     8820

ctcctttgcc aagatgggca cccagtctgg cttcctgttt tacgtgcctg ccccatatac     8880

atctaagatc gatcccctga ccggcttcgt ggaccccttc gtgtggaaaa ccatcaagaa     8940

tcacgagagc cgcaagcact tcctggaggg cttcgacttt ctgcactacg acgtgaaaac     9000

cggcgacttc atcctgcact ttaagatgaa cagaaatctg tccttccaga ggggcctgcc     9060

cggctttatg cctgcatggg atatcgtgtt cgagaagaac gagacacagt ttgacgccaa     9120

gggcacccct ttcatcgccg gcaagagaat cgtgccagtg atcgagaatc acagattcac     9180

cggcagatac cgggacctgt atcctgccaa cgagctgatc gccctgctgg aggagaaggg     9240

catcgtgttc agggatggct ccaacatcct gccaaagctg ctggagaatg acgattctca     9300

cgccatcgac accatggtgg ccctgatccg cagcgtgctg cagatgcgga actccaatgc     9360

cgccacaggc gaggactata tcaacagccc cgtgcgcgat ctgaatggcg tgtgcttcga     9420

ctcccggttt cagaacccag agtggcccat ggacgccgat gccaatggcg cctaccacat     9480

cgccctgaag ggccagctgc tgctgaatca cctgaaggag agcaaggatc tgaagctgca     9540

gaacggcatc tccaatcagg actggctggc ctacatccag gagctgcgca acaaaaggcc     9600

ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag                           9640


<210>  32
<211>  9403
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMAL-C5XLbCpfl vector

<400>  32
gaattccctg caggtaatta aataagcttc aaataaaacg aaaggctcag tcgaaagact       60

gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc      120

cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc      180

cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt      240

ttctacaaac tctttcggtc cgttgtttat ttttctaaat acattcaaat atgtatccgc      300

tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta      360

ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg      420

ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg      480

gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac      540

gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg      600

acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt      660

actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg      720

ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac      780

cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt      840

gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag      900

caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc      960

aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc     1020

ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta     1080

tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg     1140

ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga     1200

ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttcctta     1260

ggactgagcg tcaaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     1320

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     1380

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     1440

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     1500

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     1560

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     1620

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     1680

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     1740

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     1800

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     1860

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     1920

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     1980

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     2040

cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg     2100

catctgtgcg gtatttcaca ccgcatataa ggtgcactgt gactgggtca tggctgcgcc     2160

ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     2220

ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc     2280

accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca     2340

gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg     2400

gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc     2460

cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct     2520

cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca     2580

actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt     2640

cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg     2700

gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc     2760

gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt     2820

tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg     2880

ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga     2940

aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa     3000

gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat     3060

gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg     3120

aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg     3180

gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc     3240

ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc     3300

agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac     3360

aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat     3420

gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac     3480

cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag     3540

catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc     3600

tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg     3660

atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg     3720

ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc     3780

gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatttc ggtagtggga     3840

tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat     3900

tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg     3960

gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc     4020

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag     4080

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca     4140

ttaggcacaa ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct     4200

ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa     4260

ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac     4320

ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg     4380

tggaattgtg agcggataac aatttcacac aggaaacagc cagtccgttt aggtgttttc     4440

acgagcaatt gaccaacaag gaccatagat tatgaaaatc gaagaaggta aactggtaat     4500

ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa     4560

agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca     4620

ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg     4680

ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct     4740

gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc     4800

tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg     4860

ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt     4920

caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt     4980

caagtatgaa aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa     5040

agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga     5100

ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc     5160

gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac     5220

cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc     5280

cagtccgaac aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg     5340

tctggaagcg gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga     5400

agagttggtg aaagatccgc gtattgccgc cactatggaa aacgcccaga aaggtgaaat     5460

catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa     5520

cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag     5580

ctcgaacaac aacaacaata acaataacaa caacctcggg atcgagggaa ggatttcaca     5640

tatgtccatg ggcggccgcg atatcgtcga catgagcaag ctggagaagt ttacaaactg     5700

ctactccctg tctaagaccc tgaggttcaa ggccatccct gtgggcaaga cccaggagaa     5760

catcgacaat aagcggctgc tggtggagga cgagaagaga gccgaggatt ataagggcgt     5820

gaagaagctg ctggatcgct actatctgtc ttttatcaac gacgtgctgc acagcatcaa     5880

gctgaagaat ctgaacaatt acatcagcct gttccggaag aaaaccagaa ccgagaagga     5940

gaataaggag ctggagaacc tggagatcaa tctgcggaag gagatcgcca aggccttcaa     6000

gggcaacgag ggctacaagt ccctgtttaa gaaggatatc atcgagacaa tcctgccaga     6060

gttcctggac gataaggacg agatcgccct ggtgaacagc ttcaatggct ttaccacagc     6120

cttcaccggc ttctttgata acagagagaa tatgttttcc gaggaggcca agagcacatc     6180

catcgccttc aggtgtatca acgagaatct gacccgctac atctctaata tggacatctt     6240

cgagaaggtg gacgccatct ttgataagca cgaggtgcag gagatcaagg agaagatcct     6300

gaacagcgac tatgatgtgg aggatttctt tgagggcgag ttctttaact ttgtgctgac     6360

acaggagggc atcgacgtgt ataacgccat catcggcggc ttcgtgaccg agagcggcga     6420

gaagatcaag ggcctgaacg agtacatcaa cctgtataat cagaaaacca agcagaagct     6480

gcctaagttt aagccactgt ataagcaggt gctgagcgat cgggagtctc tgagcttcta     6540

cggcgagggc tatacatccg atgaggaggt gctggaggtg tttagaaaca ccctgaacaa     6600

gaacagcgag atcttcagct ccatcaagaa gctggagaag ctgttcaaga attttgacga     6660

gtactctagc gccggcatct ttgtgaagaa cggccccgcc atcagcacaa tctccaagga     6720

tatcttcggc gagtggaacg tgatccggga caagtggaat gccgagtatg acgatatcca     6780

cctgaagaag aaggccgtgg tgaccgagaa gtacgaggac gatcggagaa agtccttcaa     6840

gaagatcggc tccttttctc tggagcagct gcaggagtac gccgacgccg atctgtctgt     6900

ggtggagaag ctgaaggaga tcatcatcca gaaggtggat gagatctaca aggtgtatgg     6960

ctcctctgag aagctgttcg acgccgattt tgtgctggag aagagcctga agaagaacga     7020

cgccgtggtg gccatcatga aggacctgct ggattctgtg aagagcttcg agaattacat     7080

caaggccttc tttggcgagg gcaaggagac aaacagggac gagtccttct atggcgattt     7140

tgtgctggcc tacgacatcc tgctgaaggt ggaccacatc tacgatgcca tccgcaatta     7200

tgtgacccag aagccctact ctaaggataa gttcaagctg tattttcaga accctcagtt     7260

catgggcggc tgggacaagg ataaggagac agactatcgg gccaccatcc tgagatacgg     7320

ctccaagtac tatctggcca tcatggataa gaagtacgcc aagtgcctgc agaagatcga     7380

caaggacgat gtgaacggca attacgagaa gatcaactat aagctgctgc ccggccctaa     7440

taagatgctg ccaaaggtgt tcttttctaa gaagtggatg gcctactata accccagcga     7500

ggacatccag aagatctaca agaatggcac attcaagaag ggcgatatgt ttaacctgaa     7560

tgactgtcac aagctgatcg acttctttaa ggatagcatc tcccggtatc caaagtggtc     7620

caatgcctac gatttcaact tttctgagac agagaagtat aaggacatcg ccggctttta     7680

cagagaggtg gaggagcagg gctataaggt gagcttcgag tctgccagca agaaggaggt     7740

ggataagctg gtggaggagg gcaagctgta tatgttccag atctataaca aggacttttc     7800

cgataagtct cacggcacac ccaatctgca caccatgtac ttcaagctgc tgtttgacga     7860

gaacaatcac ggacagatca ggctgagcgg aggagcagag ctgttcatga ggcgcgcctc     7920

cctgaagaag gaggagctgg tggtgcaccc agccaactcc cctatcgcca acaagaatcc     7980

agataatccc aagaaaacca caaccctgtc ctacgacgtg tataaggata agaggttttc     8040

tgaggaccag tacgagctgc acatcccaat cgccatcaat aagtgcccca agaacatctt     8100

caagatcaat acagaggtgc gcgtgctgct gaagcacgac gataacccct atgtgatcgg     8160

catcgatagg ggcgagcgca atctgctgta tatcgtggtg gtggacggca agggcaacat     8220

cgtggagcag tattccctga acgagatcat caacaacttc aacggcatca ggatcaagac     8280

agattaccac tctctgctgg acaagaagga gaaggagagg ttcgaggccc gccagaactg     8340

gacctccatc gagaatatca aggagctgaa ggccggctat atctctcagg tggtgcacaa     8400

gatctgcgag ctggtggaga agtacgatgc cgtgatcgcc ctggaggacc tgaactctgg     8460

ctttaagaat agccgcgtga aggtggagaa gcaggtgtat cagaagttcg agaagatgct     8520

gatcgataag ctgaactaca tggtggacaa gaagtctaat ccttgtgcaa caggcggcgc     8580

cctgaagggc tatcagatca ccaataagtt cgagagcttt aagtccatgt ctacccagaa     8640

cggcttcatc ttttacatcc ctgcctggct gacatccaag atcgatccat ctaccggctt     8700

tgtgaacctg ctgaaaacca agtataccag catcgccgat tccaagaagt tcatcagctc     8760

ctttgacagg atcatgtacg tgcccgagga ggatctgttc gagtttgccc tggactataa     8820

gaacttctct cgcacagacg ccgattacat caagaagtgg aagctgtact cctacggcaa     8880

ccggatcaga atcttccgga atcctaagaa gaacaacgtg ttcgactggg aggaggtgtg     8940

cctgaccagc gcctataagg agctgttcaa caagtacggc atcaattatc agcagggcga     9000

tatcagagcc ctgctgtgcg agcagtccga caaggccttc tactctagct ttatggccct     9060

gatgagcctg atgctgcaga tgcggaacag catcacaggc cgcaccgacg tggattttct     9120

gatcagccct gtgaagaact ccgacggcat cttctacgat agccggaact atgaggccca     9180

ggagaatgcc atcctgccaa agaacgccga cgccaatggc gcctataaca tcgccagaaa     9240

ggtgctgtgg gccatcggcc agttcaagaa ggccgaggac gagaagctgg ataaggtgaa     9300

gatcgccatc tctaacaagg agtggctgga gtacgcccag accagcgtga agcacaaaag     9360

gccggcggcc acgaaaaagg ccggccaggc aaaaaagaaa aag                       9403


<210>  33
<211>  9619
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pMAL-C5X/FnCpf1 vector

<400>  33
gaattccctg caggtaatta aataagcttc aaataaaacg aaaggctcag tcgaaagact       60

gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc      120

cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc      180

cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt      240

ttctacaaac tctttcggtc cgttgtttat ttttctaaat acattcaaat atgtatccgc      300

tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta      360

ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg      420

ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg      480

gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac      540

gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg      600

acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt      660

actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg      720

ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac      780

cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt      840

gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag      900

caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc      960

aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc     1020

ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta     1080

tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg     1140

ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga     1200

ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttcctta     1260

ggactgagcg tcaaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     1320

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     1380

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     1440

aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     1500

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     1560

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     1620

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     1680

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     1740

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     1800

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     1860

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     1920

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     1980

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     2040

cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg     2100

catctgtgcg gtatttcaca ccgcatataa ggtgcactgt gactgggtca tggctgcgcc     2160

ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc     2220

ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc     2280

accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca     2340

gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg     2400

gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc     2460

cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct     2520

cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca     2580

actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt     2640

cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg     2700

gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc     2760

gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt     2820

tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg     2880

ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga     2940

aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa     3000

gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat     3060

gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg     3120

aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg     3180

gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc     3240

ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc     3300

agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac     3360

aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat     3420

gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac     3480

cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag     3540

catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc     3600

tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg     3660

atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg     3720

ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc     3780

gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatttc ggtagtggga     3840

tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat     3900

tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg     3960

gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc     4020

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag     4080

gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt agctcactca     4140

ttaggcacaa ttctcatgtt tgacagctta tcatcgactg cacggtgcac caatgcttct     4200

ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa     4260

ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac     4320

ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg     4380

tggaattgtg agcggataac aatttcacac aggaaacagc cagtccgttt aggtgttttc     4440

acgagcaatt gaccaacaag gaccatagat tatgaaaatc gaagaaggta aactggtaat     4500

ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga aattcgagaa     4560

agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga aattcccaca     4620

ggttgcggca actggcgatg gccctgacat tatcttctgg gcacacgacc gctttggtgg     4680

ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc aggacaagct     4740

gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt acccgatcgc     4800

tgttgaagcg ttatcgctga tttataacaa agatctgctg ccgaacccgc caaaaacctg     4860

ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg cgctgatgtt     4920

caacctgcaa gaaccgtact tcacctggcc gctgattgct gctgacgggg gttatgcgtt     4980

caagtatgaa aacggcaagt acgacattaa agacgtgggc gtggataacg ctggcgcgaa     5040

agcgggtctg accttcctgg ttgacctgat taaaaacaaa cacatgaatg cagacaccga     5100

ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacca tcaacggccc     5160

gtgggcatgg tccaacatcg acaccagcaa agtgaattat ggtgtaacgg tactgccgac     5220

cttcaagggt caaccatcca aaccgttcgt tggcgtgctg agcgcaggta ttaacgccgc     5280

cagtccgaac aaagagctgg caaaagagtt cctcgaaaac tatctgctga ctgatgaagg     5340

tctggaagcg gttaataaag acaaaccgct gggtgccgta gcgctgaagt cttacgagga     5400

agagttggtg aaagatccgc gtattgccgc cactatggaa aacgcccaga aaggtgaaat     5460

catgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg cggtgatcaa     5520

cgccgccagc ggtcgtcaga ctgtcgatga agccctgaaa gacgcgcaga ctaattcgag     5580

ctcgaacaac aacaacaata acaataacaa caacctcggg atcgagggaa ggatttcaca     5640

tatgtccatg ggcggccgcg atatcgtcga catgagcatc taccaggagt tcgtcaacaa     5700

gtattcactg agtaagacac tgcggttcga gctgatccca cagggcaaga cactggagaa     5760

catcaaggcc cgaggcctga ttctggacga tgagaagcgg gcaaaagact ataagaaagc     5820

caagcagatc attgataaat accaccagtt ctttatcgag gaaattctga gctccgtgtg     5880

catcagtgag gatctgctgc agaattactc agacgtgtac ttcaagctga agaagagcga     5940

cgatgacaac ctgcagaagg acttcaagtc cgccaaggac accatcaaga aacagattag     6000

cgagtacatc aaggactccg aaaagtttaa aaatctgttc aaccagaatc tgatcgatgc     6060

taagaaaggc caggagtccg acctgatcct gtggctgaaa cagtctaagg acaatgggat     6120

tgaactgttc aaggctaact ccgatatcac tgatattgac gaggcactgg aaatcatcaa     6180

gagcttcaag ggatggacca catactttaa aggcttccac gagaaccgca agaacgtgta     6240

ctccagcaac gacattccta cctccatcat ctaccgaatc gtcgatgaca atctgccaaa     6300

gttcctggag aacaaggcca aatatgaatc tctgaaggac aaagctcccg aggcaattaa     6360

ttacgaacag atcaagaaag atctggctga ggaactgaca ttcgatatcg actataagac     6420

tagcgaggtg aaccagaggg tcttttccct ggacgaggtg tttgaaatcg ccaatttcaa     6480

caattacctg aaccagtccg gcattactaa attcaatacc atcattggcg ggaagtttgt     6540

gaacggggag aataccaagc gcaagggaat taacgaatac atcaatctgt atagccagca     6600

gatcaacgac aaaactctga agaaatacaa gatgtctgtg ctgttcaaac agatcctgag     6660

tgataccgag tccaagtctt ttgtcattga taaactggaa gatgactcag acgtggtcac     6720

taccatgcag agcttttatg agcagatcgc cgctttcaag acagtggagg aaaaatctat     6780

taaggaaact ctgagtctgc tgttcgatga cctgaaagcc cagaagctgg acctgagtaa     6840

gatctacttc aaaaacgata agagtctgac agacctgtca cagcaggtgt ttgatgacta     6900

ttccgtgatt gggaccgccg tcctggagta cattacacag cagatcgctc caaagaacct     6960

ggataatccc tctaagaaag agcaggaact gatcgctaag aaaaccgaga aggcaaaata     7020

tctgagtctg gaaacaatta agctggcact ggaggagttc aacaagcaca gggatattga     7080

caaacagtgc cgctttgagg aaatcctggc caacttcgca gccatcccca tgatttttga     7140

tgagatcgcc cagaacaaag acaatctggc tcagatcagt attaagtacc agaaccaggg     7200

caagaaagac ctgctgcagg cttcagcaga agatgacgtg aaagccatca aggatctgct     7260

ggaccagacc aacaatctgc tgcacaagct gaaaatcttc catattagtc agtcagagga     7320

taaggctaat atcctggata aagacgaaca cttctacctg gtgttcgagg aatgttactt     7380

cgagctggca aacattgtcc ccctgtataa caagattagg aactacatca cacagaagcc     7440

ttactctgac gagaagttta aactgaactt cgaaaatagt accctggcca acgggtggga     7500

taagaacaag gagcctgaca acacagctat cctgttcatc aaggatgaca agtactatct     7560

gggagtgatg aataagaaaa acaataagat cttcgatgac aaagccatta aggagaacaa     7620

aggggaagga tacaagaaaa tcgtgtataa gctgctgccc ggcgcaaata agatgctgcc     7680

taaggtgttc ttcagcgcca agagtatcaa attctacaac ccatccgagg acatcctgcg     7740

gattagaaat cactcaacac atactaagaa cgggagcccc cagaagggat atgagaaatt     7800

tgagttcaac atcgaggatt gcaggaagtt tattgacttc tacaagcaga gcatctccaa     7860

acaccctgaa tggaaggatt ttggcttccg gttttccgac acacagagat ataactctat     7920

cgacgagttc taccgcgagg tggaaaatca ggggtataag ctgacttttg agaacatttc     7980

tgaaagttac atcgacagcg tggtcaatca gggaaagctg tacctgttcc agatctataa     8040

caaagatttt tcagcataca gcaagggcag accaaacctg catacactgt actggaaggc     8100

cctgttcgat gagaggaatc tgcaggacgt ggtctataaa ctgaacggag aggccgaact     8160

gttttaccgg aagcagtcta ttcctaagaa aatcactcac ccagctaagg aggccatcgc     8220

taacaagaac aaggacaatc ctaagaaaga gagcgtgttc gaatacgatc tgattaagga     8280

caagcggttc accgaagata agttcttttt ccattgtcca atcaccatta acttcaagtc     8340

aagcggcgct aacaagttca acgacgagat caatctgctg ctgaaggaaa aagcaaacga     8400

tgtgcacatc ctgagcattg accgaggaga gcggcatctg gcctactata ccctggtgga     8460

tggcaaaggg aatatcatta agcaggatac attcaacatc attggcaatg accggatgaa     8520

aaccaactac cacgataaac tggctgcaat cgagaaggat agagactcag ctaggaagga     8580

ctggaagaaa atcaacaaca ttaaggagat gaaggaaggc tatctgagcc aggtggtcca     8640

tgagattgca aagctggtca tcgaatacaa tgccattgtg gtgttcgagg atctgaactt     8700

cggctttaag agggggcgct ttaaggtgga aaaacaggtc tatcagaagc tggagaaaat     8760

gctgatcgaa aagctgaatt acctggtgtt taaagataac gagttcgaca agaccggagg     8820

cgtcctgaga gcctaccagc tgacagctcc ctttgaaact ttcaagaaaa tgggaaaaca     8880

gacaggcatc atctactatg tgccagccgg attcacttcc aagatctgcc ccgtgaccgg     8940

ctttgtcaac cagctgtacc ctaaatatga gtcagtgagc aagtcccagg aatttttcag     9000

caagttcgat aagatctgtt ataatctgga caaggggtac ttcgagtttt ccttcgatta     9060

caagaacttc ggcgacaagg ccgctaaggg gaaatggacc attgcctcct tcggatctcg     9120

cctgatcaac tttcgaaatt ccgataaaaa ccacaattgg gacactaggg aggtgtaccc     9180

aaccaaggag ctggaaaagc tgctgaaaga ctactctatc gagtatggac atggcgaatg     9240

catcaaggca gccatctgtg gcgagagtga taagaaattt ttcgccaagc tgacctcagt     9300

gctgaataca atcctgcaga tgcggaactc aaagaccggg acagaactgg actatctgat     9360

tagccccgtg gctgatgtca acggaaactt cttcgacagc agacaggcac ccaaaaatat     9420

gcctcaggat gcagacgcca acggggccta ccacatcggg ctgaagggac tgatgctgct     9480

gggccggatc aagaacaatc aggaggggaa gaagctgaac ctggtcatta agaacgagga     9540

atacttcgag tttgtccaga atagaaataa caaaaggccg gcggccacga aaaaggccgg     9600

ccaggcaaaa aagaaaaag                                                  9619


<210>  34
<211>  1100
<212>  DNA
<213>  Citrus sinensis

<400>  34
tcctcatccc ttactgtctt tgctttctca ctaactacta caacccaaca gttttcttct       60

ctcaaaaatg gaatgcaaac acaaaattaa tgtagcaatc ccaatcacta atatgaagaa      120

cactcaattc tcatctccat ctactttctc tacttctcct ccttctcaat cttctccacg      180

cttcccttct cctaatcatc aacaattgtc ttctccagaa tcttctccaa gctttaaagc      240

ttctccttca caatcctctc caaatcttgc agctcccctc tctccgccgc ctatagttct      300

tagcccttgt gctgcttgca aaatcctccg ccgcagatgc gtcgagaaat gtgttttagc      360

tccatatttt ccaccaaccg aaccatacaa gttcaccatt gctcatagag tcttcggtgc      420

tagcaatatc atcaagttct tgcaggaact gccagaatct caacgagcag atgcagtgag      480

cagcatggtc tatgaagcaa gtgccagaat ccgggatcct gtttacggct gcgccggggc      540

tatttgccat ctccagaaac aagtcagtga gcttcaggct cagttagcca aggcacaggc      600

tgagcttgtc accatggaaa gccagcaacg caatttaata actctaattt gcatggaaat      660

ggcacaatct caagaacaag tcttgcagca gcagcagcag cagcagcaac agttcatgga      720

tactagctgt tttttggatg acaatggtat tggatcagct tgggagcctc tgtggacatg      780

atcaagagaa attaaagcaa gattgttgaa attttaacct tttaagagat tatttacata      840

aagctaaaca tacttaatta taaaagtttc tgatcaataa ttaaagttat ttgctgcgcg      900

gtagatggga gtgattatta tgtgctttaa ttttcattag tcttgttgac aaaaaggaat      960

ctttgaacca tctggagaag tcctttgtta acggttcgag attaattatt agtttatctt     1020

tatttacatt tagtgaaatt ttgtttttaa ctaattttat agacataaat aacccaacca     1080

agatgggaat tcagtgctga                                                 1100


<210>  35
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS-349

<400>  35
ggtgctgttt tgtcagggaa gctttgtgca caagcaattg tacaggtaca ttatagttct       60

gg                                                                      62


<210>  36
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS-349 2g

<400>  36
aaggagctgt tttgtcaggg acgaatgcca aaggacagga cattatagtt ctgg             54


<210>  37
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS-349 3g

<400>  37
ggtgctgttg tgtctggggg gctttgtggc acgacaattg tacaggtgca ttatagttct       60

gg                                                                      62


<210>  38
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS-349 3b

<400>  38
ggtgctgttt tgtcagggaa ttttagtgcc aaacaattgt acaggtacat tatagttctg       60

g                                                                       61


<210>  39
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS-349 1J

<400>  39
tggaaggtgc tgttttgtca gggaagcttt gtgctaagca attgtacagg tacattatag       60

ttctggtgt                                                               69


<210>  40
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS-349 3J

<400>  40
tggaaggtcg cgggtcttgt cagggaagct ttgggccccg gtattggaca ggtacattat       60

agttctggtg                                                              70


<210>  41
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS-349 7J

<400>  41
tgttgggtgc ggttctgtcg ggaactttgt gccaagcaac tgacaggtac attatagttc       60

tggtg                                                                   65


<210>  42
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDS349 9J

<400>  42
tgttttgtca gggaagcttt gtgccaagca attggacagg tacattatag ttctggtg         58


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOB1-sgRNA6

<400>  43
cctatagttc ttagcccttg tgc                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  csLOB1 target site

<400>  44
gcacaagggc taagaactat agg                                               23


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOB1 target site mutant M1

<400>  45
gcacaagggc taagaacata tagg                                              24


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOB1 target site mutant M2

<400>  46
gcacaagggc taagatatag g                                                 21


<210>  47
<211>  7
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOB1 target site mutant M3

<400>  47
gcacagg                                                                  7


<210>  48
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOB1 target site mutant M4

<400>  48
gcacaagggc taagaatata gg                                                22


<210>  49
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOB1 target site mutant M5

<400>  49
gcacaagggc taatatagg                                                    19


<210>  50
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target site

<400>  50
gcacaagggc taagaactat aggcggcg                                          28


<210>  51
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  type 1 insertion +1 G

<400>  51
gcacaagggc taagaacgta taggcggcg                                         29


<210>  52
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type 1 insertion +1T

<400>  52
gcacaagggc taagaactta taggcggcg                                         29


<210>  53
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  type 1 Insertion +1A

<400>  53
gcacaagggc taagaacata taggcggcg                                         29


<210>  54
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion -1T

<400>  54
gcacaagggc taagaacata ggcggcg                                           27


<210>  55
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion -1 C

<400>  55
gcacaagggc taagaatata ggcggcg                                           27


<210>  56
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II delection -2 CT

<400>  56
gcacaagggc taagaaatag gcggcg                                            26


<210>  57
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II delection -2 AC

<400>  57
gcacaagggc taagatatag gcggcg                                            26


<210>  58
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II Delection -TA

<400>  58
gcacaagggc taagaactag gcggcg                                            26


<210>  59
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II delection -3 ACT

<400>  59
gcacaagggc taagaatagg cggcg                                             25


<210>  60
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion -3 GAA, C

<400>  60
gcacaagggc taaatatagg cggcg                                             25


<210>  61
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Typye II deletion -3 CTA

<400>  61
gcacaagggc taagaatagg cggcg                                             25


<210>  62
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II delection -3 AAC

<400>  62
gcacaagggc taagtatagg cggcg                                             25


<210>  63
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion -4 GACC

<400>  63
gcacaagggc taatataggc ggcg                                              24


<210>  64
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion -5 AGAAC

<400>  64
gcacaagggc tatataggcg gcg                                               23


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion -5 GAACT

<400>  65
gcacaagggc taaataggcg gcg                                               23


<210>  66
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion, -6 AGAACT

<400>  66
gcacaagggc taataggcgg cg                                                22


<210>  67
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion, -6 AAGAAC

<400>  67
gcacaagggc ttataggcgg cg                                                22


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion, -8 TAAGAACT

<400>  68
gcacaagggc ataggcggcg                                                   20


<210>  69
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II deletion,  -16 AGGGCTAAGAACTATA

<400>  69
gcacaggcgg cg                                                           12


<210>  70
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type III change, A   G

<400>  70
gcacaagggc taagaactgt aggcggcg                                          28


<210>  71
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type III change, T   C

<400>  71
gcacaagggc taagaactac aggcggcg                                          28


<210>  72
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsPDS

<400>  72
tttcaggtaa ctgaagcttg aggatat                                           27


<210>  73
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EBE-pthA4

<400>  73
tttcctttct ctatataaac ccctttt                                           27


<210>  74
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LbCas-12a-crRNA-cspds indel (wild type)

<400>  74
tctttccagt tttcaggtaa ctgaagcttg aggatatcaa ctgaagg                     47


<210>  75
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LbCas12a-crRNA-cspds indel

<400>  75
tctttccagt tttcaggtaa ctgaagcatc aactgaagg                              39


<210>  76
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LbCas12a-crRNA-cspds indel

<400>  76
tctttccagt tttcaggtaa ctgaagctca actgaagg                               38


<210>  77
<211>  288
<212>  DNA
<213>  Citrus sinensis

<400>  77
cgtcattcaa ttaaaattaa tgacgtcatc tagtggctcg gtgacatacg ctttagatac       60

aattgtcatt cttgcctttt cctttctcta tataaacccc ttttgccttg aactttgttt      120

caactaaagc agctcctcct catcccttac tgtctttgct ttctcactaa ctactacaac      180

ccaacagttt tcttctctca aaaatggaat gcaaacacaa aattaatgta gcaatcccaa      240

tcactaatat gaagaacact caattctcat ctccatctac tttctcta                   288


<210>  78
<211>  287
<212>  DNA
<213>  Citrus sinensis

<400>  78
cgtcattcaa ttaaaattaa tgacgtcatc tagtggcttg gtgacatacg ctttagatac       60

aattgtcatt cttgcctttt cctttctcta tataaacccc ttttgcctta actttgtttc      120

aactaaagca gctcctcctc atcccttact gtcttcgctt tctcactaac tactacaacc      180

caacagtttt cttctctcaa aaatggaatg cagacacaaa attaatgtag caatcccaat      240

cactaatatg aagaatactc aattctcatc tccatctact ttctcta                    287


<210>  79
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chromatogram

<400>  79
attcttgcct tttcctttct ctatataaac cccttttgcc ttgactt                     47


<210>  80
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chromotogram

<400>  80
attcttgcct tttcctttct ctatataaac ccctttggct ttgaact                     47


<210>  81
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP target site wild-type

<400>  81
attcttgcct tttcctttct ctatataaac cccttttgcc ttgaact                     47


<210>  82
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP mutation

<400>  82
attcttgcct tttcctttct ctatataaac cccttttgcc ttaact                      46


<210>  83
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP mutation

<400>  83
attcttgcct tttcctttct ctatataaac cttgaact                               38


<210>  84
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  csLOBP mutation

<400>  84
attcttgcct tttcctttct ctatataaac ccctttgcct taact                       45


<210>  85
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  85
attcttgcct tttcctttct ctatataaac cccttttgcc ttgaact                     47


<210>  86
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  86
attcttgcct tttcctttct ctatataaac cccttttgcc ttaact                      46


<210>  87
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  87
attcttgcct tttcctttct ctatataaac cttttgcctt gaact                       45


<210>  88
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  88
attcttgcct tttcctttct ctatataaac ccctttgcct taact                       45


<210>  89
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  89
attcttgcct tttcctttct ctatataaac cccttttgcc ttgaact                     47


<210>  90
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  90
attcttgcct tttcctttct ctatataaac cccttttgcc ttaact                      46


<210>  91
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  91
attcttgcct tttcctttct ctatatattg aact                                   34


<210>  92
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  92
attcttgcct tttcctttct ctatataaag gccttgaact                             40


<210>  93
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP indels in transgenic Duncan #D35s1

<400>  93
attcttgcct tttcctttct gtaccttgaa ct                                     32


<210>  94
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial dTALE dCsLOB1.4

<400>  94
tttctctata taaacccctt ttgccttaac tttgtatg                               38


<210>  95
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  artificial dTALE dCsLOB1.4

<400>  95
aaaccccttt tgccttaact t                                                 21


<210>  96
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA spacer

<400>  96
cagcagcagc agcagcagca gcaac                                             25


<210>  97
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  crRNA spacer

<400>  97
cggctgcgcc ggggctattt gcca                                              24


<210>  98
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  98
tggcaatgtg attgacggag atgc                                              24


<210>  99
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  99
atgagtcctc cttgttactt cagt                                              24


<210>  100
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  100
aggtaagctt attcatatta acgttatcaa tgatt                                  35


<210>  101
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  101
acctggatcc ttttgagaga agaaaactgt tgggt                                  35


<210>  102
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  102
cgtcattcaa ttaaaattaa tgac                                              24


<210>  103
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  103
cagcagcagc agcagcagca gcaac                                             25


<210>  104
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  104
cggctgcgcc ggggctattt gcca                                              24


<210>  105
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  105
aggtcctgca ggtccccaga ttagcctttt caattt                                 36


<210>  106
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  106
aggtggatcc ggtacctatc gttcgtaaat ggtgaaaatt                             40


<210>  107
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  107
aggtcctgca ggatgggaaa ttcattgaaa accct                                  35


<210>  108
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  108
aggtggtacc ggatcctttc ttcttctcgt tgttgtactt cat                         43


<210>  109
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  109
aggatccacc ggtgcacgaa ttccgaattt ccccgatcgt tcaa                        44


<210>  110
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  110
agtttaaact ctagacaagg cgcgccattt aaatctcgag ccgatctagt aacatagatg       60

acac                                                                    64


<210>  111
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  111
actcgagact agtaccatgg tggactcctc ttaa                                   34


<210>  112
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  113

<400>  112
ctacacttag tagaaattcc tctccaaatg aaatgaactt cct                         43


<210>  113
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  113
ataggtaact gaagcttgag gatatgaatt tccccgatcg ttcaaacatt tg               52


<210>  114
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  114
acctgggccc ggcgcgccga tctagtaaca tagatga                                37


<210>  115
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  115
atctttctct atataaaccc cttttgaatt tccccgatcg ttcaaacatt tg               52


<210>  116
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  116
aggtggcgcg cctcttacag cttagaaatc tcaaa                                  35


<210>  117
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  117
ctacacttag tagaaattca atcactactt cgtctctaac catata                      46


<210>  118
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  118
aggtactagt ccgatctagt aacatagatg aca                                    33


<210>  119
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  119
tgtgctcgac gttgtcactg aagc                                              24


<210>  120
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  120
ttcgggggat ctggatttta gtac                                              24


<210>  121
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOB1.1 binding site

<400>  121
taaagcagct cctcctc                                                      17


<210>  122
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCsLOB1.2 recognition sequence

<400>  122
tataaacccc ttttgcctt                                                    19


<210>  123
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCsLOB1.3 binding sequence

<400>  123
ccttttgcct tgaacttt                                                     18


<210>  124
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsLOBP binding sequence

<400>  124
ccgcgatatc gtcgacatga cacagttcga gggc                                   34


<210>  125
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AtU6-26 promoter

<400>  125
tacctgcagg gaattccttt ttcttttttg cctggc                                 36


<210>  126
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsU6-2 promoter

<400>  126
ccgcgatatc gtcgacatga gcaagctgga gaagt                                  35


<210>  127
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsUg-7 promoter

<400>  127
tacctgcagg gaattccttt ttcttttttg cctggc                                 36


<210>  128
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  128
ccgcgatatc gtcgacatga gcatctacca ggagt                                  35


<210>  129
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  129
tacctgcagg gaattccttt ttcttttttg cctggc                                 36


<210>  130
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  130
cgcagatgcg tcgagaaatg                                                   20


<210>  131
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  131
ggctcccaag ctgatccaat                                                   20


<210>  132
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  132
gcagtgagca gcatggtcta                                                   20


<210>  133
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  133
accgcgcagc aaataacttt                                                   20


<210>  134
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  134
tctccgccgc ctatagttct                                                   20


<210>  135
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  135
atcatgtcca cagaggctcc                                                   20


<210>  136
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  136
tctctccgcc gcctatagtt                                                   20


<210>  137
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  137
atgtccacag aggctcccaa                                                   20


<210>  138
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  138
gaagaacact caattctcat ctcc                                              24


<210>  139
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  139
gtccacagag gctcccaag                                                    19


<210>  140
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cs7g27620

<400>  140
gcgcatggac taagaacaat agg                                               23


<210>  141
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cs7g27620

<400>  141
gcgcatggac taagaacaat aag                                               23


<210>  142
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cs7g27620

<400>  142
gcgcatggac caagaacaat aag                                               23


<210>  143
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cs7g27620

<400>  143
gcgcgtggac taagaacaat agg                                               23


<210>  144
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cs7g02090

<400>  144
gcacaagatc taagaacatt aag                                               23


<210>  145
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  orange1.1t01786

<400>  145
tcacaaggac caagaagtat tgg                                               23


<210>  146
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cs2g10860

<400>  146
gcagaaaggg taagagctat aag                                               23


<210>  147
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cs2610860 Off-target No.

<400>  147
acagaaaggg taagagctat aag                                               23


<210>  148
<211>  184
<212>  DNA
<213>  Arabidopsis thaliana

<400>  148
caatcttcaa aagtcccaca tcgcttagat aagaaaacga agctgagttt atatacagct       60

agagtcgaag tagtgattgt cccttagggg acatccgata aaattggaac gatacagaga      120

agattagcat ggcccctgcg caaggatgac acgcataaat cgagaaatgg tccaaatttt      180

tttt                                                                   184


<210>  149
<211>  183
<212>  DNA
<213>  Citrus sinensis

<400>  149
ccgttgaaga aaatcccaca tcgaaagaaa aaactgaata aacatggtct atatatacaa       60

ggactcccag gttggttgtc tcttcggaga catccgataa aattggaacg atacagagaa      120

gattagcatg gcccctgcgc aaggatgaca cgcacaaatc gagaaatggt ccaaattttt      180

ttt                                                                    183


<210>  150
<211>  183
<212>  DNA
<213>  Citrus sinensis

<400>  150
aaattgaact aaatcccaca tcgtctagtt gcgcttcgtt gagagtctta atataatcat       60

aaccaaccag tgcgattgtc tcttcggaga catccgataa aattggaacg atacagagaa      120

gattagcatg gcccctgcgc aaggatgaca cgcacaaatc gagaaatggt ccaaattttt      180

ttt                                                                    183


<210>  151
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Type II CsLOBP repeat variable di-residues

<400>  151
taaacccctt ttgccttaac tt                                                22


<210>  152
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  152
cagctcctcc tcatccctta c                                                 21


<210>  153
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  153
agtggaacaa tcaaccactc caa                                               23


<210>  154
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  154
agctcctcct catcccttac                                                   20


<210>  155
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  155
accactccaa agtctaatca caca                                              24


<210>  156
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  156
agcgtttgct tcgtagacca                                                   20


<210>  157
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  157
tatggggcta gccaatcacg                                                   20


<210>  158
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  158
tcagaatcac gtctgcacca                                                   20


<210>  159
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  159
gtgaaaaccc acaacccgc                                                    19


<210>  160
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  160
aaacgtgcat aaccacccct                                                   20


<210>  161
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  161
atctggttga tcgcatggct                                                   20


<210>  162
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  162
atggatgcgt tcaggggaag                                                   20


<210>  163
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  163
ataggcccaa gaatgtgcaa                                                   20


