                         SEQUENCE LISTING

<110>  Agency for Science, Technology and Research
 
<120>  METHOD OF DETECTING PROTEIN-PROTEIN INTERACTIONS

<130>  9869SG5917

<160>  94    

<170>  PatentIn version 3.5

<210>  1
<211>  544
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated Taq polymerase

<400>  1

Met Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro Pro Pro Glu Gly 
1               5                   10                  15      


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
            20                  25                  30          


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
        35                  40                  45              


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
    50                  55                  60                  


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
65                  70                  75                  80  


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
                85                  90                  95      


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
            100                 105                 110         


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
        115                 120                 125             


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
    130                 135                 140                 


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
145                 150                 155                 160 


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
                165                 170                 175     


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
            180                 185                 190         


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
        195                 200                 205             


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
    210                 215                 220                 


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
225                 230                 235                 240 


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
                245                 250                 255     


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
            260                 265                 270         


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
        275                 280                 285             


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
    290                 295                 300                 


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
305                 310                 315                 320 


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
                325                 330                 335     


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
            340                 345                 350         


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
        355                 360                 365             


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
    370                 375                 380                 


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
385                 390                 395                 400 


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
                405                 410                 415     


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
            420                 425                 430         


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
        435                 440                 445             


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
    450                 455                 460                 


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
465                 470                 475                 480 


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
                485                 490                 495     


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
            500                 505                 510         


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
        515                 520                 525             


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
    530                 535                 540                 


<210>  2
<211>  852
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  2

Met Lys Ser Gly Arg Gln Glu Arg Ser Glu Glu Glu Trp Lys Glu Trp 
1               5                   10                  15      


Leu Glu Arg Lys Val Gly Glu Gly Arg Ala Arg Arg Leu Ile Glu Tyr 
            20                  25                  30          


Phe Gly Ser Ala Gly Glu Val Gly Lys Leu Val Glu Asn Ala Glu Val 
        35                  40                  45              


Ser Lys Leu Leu Glu Val Pro Gly Ile Gly Asp Glu Ala Val Ala Arg 
    50                  55                  60                  


Leu Val Pro Gly Tyr Lys Thr Leu Arg Asp Ala Gly Leu Thr Pro Ala 
65                  70                  75                  80  


Glu Ala Glu Arg Val Leu Lys Arg Tyr Gly Ser Val Ser Lys Val Gln 
                85                  90                  95      


Glu Gly Ala Thr Pro Asp Glu Leu Arg Glu Leu Gly Leu Gly Asp Ala 
            100                 105                 110         


Lys Ile Ala Arg Ile Leu Gly Leu Arg Ser Leu Val Asn Lys Arg Leu 
        115                 120                 125             


Asp Val Asp Thr Ala Tyr Glu Leu Lys Arg Arg Tyr Gly Ser Val Ser 
    130                 135                 140                 


Ala Val Arg Lys Ala Pro Val Lys Glu Leu Arg Glu Leu Gly Leu Ser 
145                 150                 155                 160 


Asp Arg Lys Ile Ala Arg Ile Lys Gly Ile Pro Glu Thr Met Leu Gln 
                165                 170                 175     


Val Arg Gly Met Ser Val Glu Lys Ala Glu Arg Leu Leu Glu Arg Phe 
            180                 185                 190         


Asp Thr Trp Thr Lys Val Lys Glu Ala Pro Val Ser Glu Leu Val Arg 
        195                 200                 205             


Val Pro Gly Val Gly Leu Ser Leu Val Lys Glu Ile Lys Ala Gln Val 
    210                 215                 220                 


Asp Pro Ala Trp Lys Ala Leu Leu Asp Val Lys Gly Val Ser Pro Glu 
225                 230                 235                 240 


Leu Ala Asp Arg Leu Val Glu Glu Leu Gly Ser Pro Tyr Arg Val Leu 
                245                 250                 255     


Thr Ala Lys Lys Ser Asp Leu Met Arg Val Glu Arg Val Gly Pro Lys 
            260                 265                 270         


Leu Ala Glu Arg Ile Arg Ala Ala Gly Lys Arg Tyr Val Glu Glu Arg 
        275                 280                 285             


Arg Ser Arg Arg Glu Arg Ile Arg Arg Lys Leu Arg Gly Ala Tyr Asp 
    290                 295                 300                 


Val Gly Gly Gly Met Ser Pro Lys Ala Leu Glu Glu Ala Pro Trp Pro 
305                 310                 315                 320 


Pro Pro Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro 
                325                 330                 335     


Met Trp Ala Asp Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val 
            340                 345                 350         


His Arg Ala Pro Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala 
        355                 360                 365             


Arg Gly Leu Leu Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly 
    370                 375                 380                 


Leu Gly Leu Pro Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu 
385                 390                 395                 400 


Asp Pro Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly 
                405                 410                 415     


Glu Trp Thr Glu Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu 
            420                 425                 430         


Phe Ala Asn Leu Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp 
        435                 440                 445             


Leu Tyr Arg Glu Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met 
    450                 455                 460                 


Glu Ala Thr Gly Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser 
465                 470                 475                 480 


Leu Glu Val Ala Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg 
                485                 490                 495     


Leu Ala Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg 
            500                 505                 510         


Val Leu Phe Asp Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys 
        515                 520                 525             


Thr Gly Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu 
    530                 535                 540                 


Ala His Pro Ile Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys 
545                 550                 555                 560 


Leu Lys Ser Thr Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg 
                565                 570                 575     


Thr Gly Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly 
            580                 585                 590         


Arg Leu Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr 
        595                 600                 605             


Pro Leu Gly Gln Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp 
    610                 615                 620                 


Leu Leu Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala 
625                 630                 635                 640 


His Leu Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg 
                645                 650                 655     


Asp Ile His Thr Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu 
            660                 665                 670         


Ala Val Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly 
        675                 680                 685             


Val Leu Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile 
    690                 695                 700                 


Pro Tyr Glu Glu Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe 
705                 710                 715                 720 


Pro Lys Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg 
                725                 730                 735     


Arg Gly Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp 
            740                 745                 750         


Leu Glu Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala 
        755                 760                 765             


Phe Asn Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala 
    770                 775                 780                 


Met Val Lys Leu Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu 
785                 790                 795                 800 


Leu Gln Val His Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala 
                805                 810                 815     


Glu Ala Val Ala Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro 
            820                 825                 830         


Leu Ala Val Pro Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu 
        835                 840                 845             


Ser Ala Lys Glu 
    850         


<210>  3
<211>  610
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  3

Met Ala Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp 
1               5                   10                  15      


Ile Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe 
            20                  25                  30          


Thr Tyr Asp Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu 
        35                  40                  45              


Lys Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys 
    50                  55                  60                  


Gly Ser Leu Pro Glu Thr Gly Gly Gly Glu Ala Pro Trp Pro Pro Pro 
65                  70                  75                  80  


Glu Gly Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp 
                85                  90                  95      


Ala Asp Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg 
            100                 105                 110         


Ala Pro Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly 
        115                 120                 125             


Leu Leu Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly 
    130                 135                 140                 


Leu Pro Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro 
145                 150                 155                 160 


Ser Asn Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp 
                165                 170                 175     


Thr Glu Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala 
            180                 185                 190         


Asn Leu Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr 
        195                 200                 205             


Arg Glu Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala 
    210                 215                 220                 


Thr Gly Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu 
225                 230                 235                 240 


Val Ala Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala 
                245                 250                 255     


Gly His Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu 
            260                 265                 270         


Phe Asp Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly 
        275                 280                 285             


Lys Arg Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His 
    290                 295                 300                 


Pro Ile Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys 
305                 310                 315                 320 


Ser Thr Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly 
                325                 330                 335     


Arg Leu His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu 
            340                 345                 350         


Ser Ser Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu 
        355                 360                 365             


Gly Gln Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu 
    370                 375                 380                 


Val Ala Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu 
385                 390                 395                 400 


Ser Gly Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile 
                405                 410                 415     


His Thr Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val 
            420                 425                 430         


Asp Pro Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu 
        435                 440                 445             


Tyr Gly Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr 
    450                 455                 460                 


Glu Glu Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys 
465                 470                 475                 480 


Val Arg Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly 
                485                 490                 495     


Tyr Val Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu 
            500                 505                 510         


Ala Arg Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn 
        515                 520                 525             


Met Pro Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val 
    530                 535                 540                 


Lys Leu Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln 
545                 550                 555                 560 


Val His Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala 
                565                 570                 575     


Val Ala Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala 
            580                 585                 590         


Val Pro Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala 
        595                 600                 605             


Lys Glu 
    610 


<210>  4
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  4

Met Gly Ser Ser His His His His His His Ser Gln Asp Pro Ala Thr 
1               5                   10                  15      


Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp Ile Ser Lys 
            20                  25                  30          


Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe Thr Tyr Asp 
        35                  40                  45              


Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys Asp Ala 
    50                  55                  60                  


Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys Gly Ser Gly 
65                  70                  75                  80  


Gly Gly Ser Leu Pro Glu Thr Gly Gly Ser Val Asp Asp Ser Ala Thr 
                85                  90                  95      


His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala Gly 
            100                 105                 110         


Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr Trp 
        115                 120                 125             


Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys Tyr 
    130                 135                 140                 


Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr Ala 
145                 150                 155                 160 


Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly 
                165                 170                 175 


<210>  5
<211>  560
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  5

Met Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly 
1               5                   10                  15      


Ser Thr Ser Ser Met Gly Gly Glu Ala Pro Trp Pro Pro Pro Glu Gly 
            20                  25                  30          


Ala Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp 
        35                  40                  45              


Leu Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro 
    50                  55                  60                  


Glu Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu 
65                  70                  75                  80  


Ala Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro 
                85                  90                  95      


Pro Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn 
            100                 105                 110         


Thr Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu 
        115                 120                 125             


Glu Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu 
    130                 135                 140                 


Trp Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu 
145                 150                 155                 160 


Val Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly 
                165                 170                 175     


Val Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala 
            180                 185                 190         


Glu Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His 
        195                 200                 205             


Pro Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp 
    210                 215                 220                 


Glu Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg 
225                 230                 235                 240 


Ser Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile 
                245                 250                 255     


Val Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr 
            260                 265                 270         


Tyr Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu 
        275                 280                 285             


His Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser 
    290                 295                 300                 


Ser Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln 
305                 310                 315                 320 


Arg Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala 
                325                 330                 335     


Leu Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly 
            340                 345                 350         


Asp Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr 
        355                 360                 365             


Glu Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro 
    370                 375                 380                 


Leu Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly 
385                 390                 395                 400 


Met Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu 
                405                 410                 415     


Ala Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg 
            420                 425                 430         


Ala Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val 
        435                 440                 445             


Glu Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg 
    450                 455                 460                 


Val Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro 
465                 470                 475                 480 


Val Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu 
                485                 490                 495     


Phe Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His 
            500                 505                 510         


Asp Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala 
        515                 520                 525             


Arg Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro 
    530                 535                 540                 


Leu Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 555                 560 


<210>  6
<211>  64
<212>  PRT
<213>  Saccharolobus solfataricus

<400>  6

Met Ala Thr Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp 
1               5                   10                  15      


Ile Ser Lys Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe 
            20                  25                  30          


Thr Tyr Asp Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu 
        35                  40                  45              


Lys Asp Ala Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys 
    50                  55                  60                  


<210>  7
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  7

Met Gly Ser Ser His His His His His His Ser Gln Asp Pro Ala Thr 
1               5                   10                  15      


Val Lys Phe Lys Tyr Lys Gly Glu Glu Lys Glu Val Asp Ile Ser Lys 
            20                  25                  30          


Ile Lys Lys Val Trp Arg Val Gly Lys Met Ile Ser Phe Thr Tyr Asp 
        35                  40                  45              


Glu Gly Gly Gly Lys Thr Gly Arg Gly Ala Val Ser Glu Lys Asp Ala 
    50                  55                  60                  


Pro Lys Glu Leu Leu Gln Met Leu Glu Lys Gln Lys Lys Gly Ser Gly 
65                  70                  75                  80  


Gly Gly Ser Leu Pro Glu Thr Gly Gly Ser Val Asp Met Val Phe Thr 
                85                  90                  95      


Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr Ala Ala Tyr Asn Leu 
            100                 105                 110         


Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu Leu Gln Asn Leu 
        115                 120                 125             


Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val Arg Ser Gly Glu Asn 
    130                 135                 140                 


Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly Leu Ser 
145                 150                 155                 160 


Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe Lys Val Val Tyr Pro 
                165                 170                 175     


Val Asp Asp His His Phe Lys Val Ile Leu Pro Tyr Gly Thr Leu Val 
            180                 185                 190         


Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr Phe Gly Arg Pro Tyr 
        195                 200                 205             


Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr Gly Thr 
    210                 215                 220                 


Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Thr Pro Asp 
225                 230                 235                 240 


Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
                245                 250     


<210>  8
<211>  557
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  8

Met Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser Gly Ser Thr Ser 
1               5                   10                  15      


Ser Met Gly Gly Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val 
            20                  25                  30          


Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala 
        35                  40                  45              


Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr 
    50                  55                  60                  


Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp 
65                  70                  75                  80  


Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp 
                85                  90                  95      


Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro 
            100                 105                 110         


Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly 
        115                 120                 125             


Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg 
    130                 135                 140                 


Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg 
145                 150                 155                 160 


Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu 
                165                 170                 175     


Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile 
            180                 185                 190         


Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn 
        195                 200                 205             


Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly 
    210                 215                 220                 


Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser 
225                 230                 235                 240 


Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys 
                245                 250                 255     


Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp 
            260                 265                 270         


Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg 
        275                 280                 285             


Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro 
    290                 295                 300                 


Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg 
305                 310                 315                 320 


Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr 
                325                 330                 335     


Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu Asn 
            340                 345                 350         


Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala 
        355                 360                 365             


Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg 
    370                 375                 380                 


Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala 
385                 390                 395                 400 


His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala 
                405                 410                 415     


Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Ile 
            420                 425                 430         


Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu 
        435                 440                 445             


Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val Lys Ser 
    450                 455                 460                 


Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly 
465                 470                 475                 480 


Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg 
                485                 490                 495     


Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu 
            500                 505                 510         


Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu Ala 
        515                 520                 525             


Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu Val 
    530                 535                 540                 


Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 555         


<210>  9
<211>  557
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  9

Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu Gly Ser Thr Ser 
1               5                   10                  15      


Ser Met Gly Gly Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val 
            20                  25                  30          


Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala 
        35                  40                  45              


Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr 
    50                  55                  60                  


Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp 
65                  70                  75                  80  


Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp 
                85                  90                  95      


Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro 
            100                 105                 110         


Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly 
        115                 120                 125             


Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg 
    130                 135                 140                 


Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg 
145                 150                 155                 160 


Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu 
                165                 170                 175     


Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile 
            180                 185                 190         


Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn 
        195                 200                 205             


Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly 
    210                 215                 220                 


Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser 
225                 230                 235                 240 


Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys 
                245                 250                 255     


Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp 
            260                 265                 270         


Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg 
        275                 280                 285             


Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro 
    290                 295                 300                 


Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg 
305                 310                 315                 320 


Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr 
                325                 330                 335     


Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu Asn 
            340                 345                 350         


Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala 
        355                 360                 365             


Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg 
    370                 375                 380                 


Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala 
385                 390                 395                 400 


His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala 
                405                 410                 415     


Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Ile 
            420                 425                 430         


Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu 
        435                 440                 445             


Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val Lys Ser 
    450                 455                 460                 


Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly 
465                 470                 475                 480 


Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg 
                485                 490                 495     


Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu 
            500                 505                 510         


Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu Ala 
        515                 520                 525             


Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu Val 
    530                 535                 540                 


Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 555         


<210>  10
<211>  557
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  10

Met Val Thr Gly Tyr Arg Leu Phe Glu Lys Ile Ser Gly Ser Thr Ser 
1               5                   10                  15      


Ser Met Gly Gly Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe Val 
            20                  25                  30          


Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu Ala 
        35                  40                  45              


Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro Tyr 
    50                  55                  60                  


Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys Asp 
65                  70                  75                  80  


Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly Asp 
                85                  90                  95      


Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr Pro 
            100                 105                 110         


Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala Gly 
        115                 120                 125             


Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly Arg 
    130                 135                 140                 


Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu Arg 
145                 150                 155                 160 


Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg Leu 
                165                 170                 175     


Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu Ile 
            180                 185                 190         


Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe Asn 
        195                 200                 205             


Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu Gly 
    210                 215                 220                 


Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr Ser 
225                 230                 235                 240 


Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu Lys 
                245                 250                 255     


Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile Asp 
            260                 265                 270         


Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr Arg 
        275                 280                 285             


Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp Pro 
    290                 295                 300                 


Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile Arg 
305                 310                 315                 320 


Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp Tyr 
                325                 330                 335     


Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu Asn 
            340                 345                 350         


Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr Ala 
        355                 360                 365             


Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met Arg 
    370                 375                 380                 


Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser Ala 
385                 390                 395                 400 


His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln Ala 
                405                 410                 415     


Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp Ile 
            420                 425                 430         


Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr Leu 
        435                 440                 445             


Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val Lys Ser 
    450                 455                 460                 


Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln Gly 
465                 470                 475                 480 


Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro Arg 
                485                 490                 495     


Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu Leu 
            500                 505                 510         


Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu Ala 
        515                 520                 525             


Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu Val 
    530                 535                 540                 


Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 555         


<210>  11
<211>  558
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  11

Met Asn Val Thr Gly Tyr Arg Leu Phe Lys Lys Ile Ser Gly Ser Thr 
1               5                   10                  15      


Ser Ser Met Gly Gly Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe 
            20                  25                  30          


Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu 
        35                  40                  45              


Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro 
    50                  55                  60                  


Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys 
65                  70                  75                  80  


Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly 
                85                  90                  95      


Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr 
            100                 105                 110         


Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala 
        115                 120                 125             


Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly 
    130                 135                 140                 


Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu 
145                 150                 155                 160 


Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg 
                165                 170                 175     


Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu 
            180                 185                 190         


Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe 
        195                 200                 205             


Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu 
    210                 215                 220                 


Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr 
225                 230                 235                 240 


Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu 
                245                 250                 255     


Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile 
            260                 265                 270         


Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr 
        275                 280                 285             


Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
    290                 295                 300                 


Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile 
305                 310                 315                 320 


Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp 
                325                 330                 335     


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            340                 345                 350         


Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr 
        355                 360                 365             


Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met 
    370                 375                 380                 


Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser 
385                 390                 395                 400 


Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln 
                405                 410                 415     


Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp 
            420                 425                 430         


Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr 
        435                 440                 445             


Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val Lys 
    450                 455                 460                 


Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln 
465                 470                 475                 480 


Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro 
                485                 490                 495     


Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu 
            500                 505                 510         


Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu 
        515                 520                 525             


Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu 
    530                 535                 540                 


Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 555             


<210>  12
<211>  558
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  12

Met Asn Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser Gly Ser Thr 
1               5                   10                  15      


Ser Ser Met Gly Gly Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala Phe 
            20                  25                  30          


Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu Leu 
        35                  40                  45              


Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu Pro 
    50                  55                  60                  


Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala Lys 
65                  70                  75                  80  


Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro Gly 
                85                  90                  95      


Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr Thr 
            100                 105                 110         


Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu Ala 
        115                 120                 125             


Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp Gly 
    130                 135                 140                 


Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val Glu 
145                 150                 155                 160 


Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly Val Arg 
                165                 170                 175     


Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu Glu 
            180                 185                 190         


Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro Phe 
        195                 200                 205             


Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu Leu 
    210                 215                 220                 


Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser Thr 
225                 230                 235                 240 


Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val Glu 
                245                 250                 255     


Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr Ile 
            260                 265                 270         


Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu His Thr 
        275                 280                 285             


Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser Asp 
    290                 295                 300                 


Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg Ile 
305                 310                 315                 320 


Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu Asp 
                325                 330                 335     


Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp Glu 
            340                 345                 350         


Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu Thr 
        355                 360                 365             


Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu Met 
    370                 375                 380                 


Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met Ser 
385                 390                 395                 400 


Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala Gln 
                405                 410                 415     


Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala Trp 
            420                 425                 430         


Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu Thr 
        435                 440                 445             


Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val Lys 
    450                 455                 460                 


Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val Gln 
465                 470                 475                 480 


Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe Pro 
                485                 490                 495     


Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His Asp Glu 
            500                 505                 510         


Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg Leu 
        515                 520                 525             


Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu Glu 
    530                 535                 540                 


Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 555             


<210>  13
<211>  559
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  fusion protein

<400>  13

Met Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala Gly Ser 
1               5                   10                  15      


Thr Ser Ser Met Gly Gly Glu Ala Pro Trp Pro Pro Pro Glu Gly Ala 
            20                  25                  30          


Phe Val Gly Phe Val Leu Ser Arg Lys Glu Pro Met Trp Ala Asp Leu 
        35                  40                  45              


Leu Ala Leu Ala Ala Ala Arg Gly Gly Arg Val His Arg Ala Pro Glu 
    50                  55                  60                  


Pro Tyr Lys Ala Leu Arg Asp Leu Lys Glu Ala Arg Gly Leu Leu Ala 
65                  70                  75                  80  


Lys Asp Leu Ser Val Leu Ala Leu Arg Glu Gly Leu Gly Leu Pro Pro 
                85                  90                  95      


Gly Asp Asp Pro Met Leu Leu Ala Tyr Leu Leu Asp Pro Ser Asn Thr 
            100                 105                 110         


Thr Pro Glu Gly Val Ala Arg Arg Tyr Gly Gly Glu Trp Thr Glu Glu 
        115                 120                 125             


Ala Gly Glu Arg Ala Ala Leu Ser Glu Arg Leu Phe Ala Asn Leu Trp 
    130                 135                 140                 


Gly Arg Leu Glu Gly Glu Glu Arg Leu Leu Trp Leu Tyr Arg Glu Val 
145                 150                 155                 160 


Glu Arg Pro Leu Ser Ala Val Leu Ala His Met Glu Ala Thr Gly Val 
                165                 170                 175     


Arg Leu Asp Val Ala Tyr Leu Arg Ala Leu Ser Leu Glu Val Ala Glu 
            180                 185                 190         


Glu Ile Ala Arg Leu Glu Ala Glu Val Phe Arg Leu Ala Gly His Pro 
        195                 200                 205             


Phe Asn Leu Asn Ser Arg Asp Gln Leu Glu Arg Val Leu Phe Asp Glu 
    210                 215                 220                 


Leu Gly Leu Pro Ala Ile Gly Lys Thr Glu Lys Thr Gly Lys Arg Ser 
225                 230                 235                 240 


Thr Ser Ala Ala Val Leu Glu Ala Leu Arg Glu Ala His Pro Ile Val 
                245                 250                 255     


Glu Lys Ile Leu Gln Tyr Arg Glu Leu Thr Lys Leu Lys Ser Thr Tyr 
            260                 265                 270         


Ile Asp Pro Leu Pro Asp Leu Ile His Pro Arg Thr Gly Arg Leu His 
        275                 280                 285             


Thr Arg Phe Asn Gln Thr Ala Thr Ala Thr Gly Arg Leu Ser Ser Ser 
    290                 295                 300                 


Asp Pro Asn Leu Gln Asn Ile Pro Val Arg Thr Pro Leu Gly Gln Arg 
305                 310                 315                 320 


Ile Arg Arg Ala Phe Ile Ala Glu Glu Gly Trp Leu Leu Val Ala Leu 
                325                 330                 335     


Asp Tyr Ser Gln Ile Glu Leu Arg Val Leu Ala His Leu Ser Gly Asp 
            340                 345                 350         


Glu Asn Leu Ile Arg Val Phe Gln Glu Gly Arg Asp Ile His Thr Glu 
        355                 360                 365             


Thr Ala Ser Trp Met Phe Gly Val Pro Arg Glu Ala Val Asp Pro Leu 
    370                 375                 380                 


Met Arg Arg Ala Ala Lys Thr Ile Asn Phe Gly Val Leu Tyr Gly Met 
385                 390                 395                 400 


Ser Ala His Arg Leu Ser Gln Glu Leu Ala Ile Pro Tyr Glu Glu Ala 
                405                 410                 415     


Gln Ala Phe Ile Glu Arg Tyr Phe Gln Ser Phe Pro Lys Val Arg Ala 
            420                 425                 430         


Trp Ile Glu Lys Thr Leu Glu Glu Gly Arg Arg Arg Gly Tyr Val Glu 
        435                 440                 445             


Thr Leu Phe Gly Arg Arg Arg Tyr Val Pro Asp Leu Glu Ala Arg Val 
    450                 455                 460                 


Lys Ser Val Arg Glu Ala Ala Glu Arg Met Ala Phe Asn Met Pro Val 
465                 470                 475                 480 


Gln Gly Thr Ala Ala Asp Leu Met Lys Leu Ala Met Val Lys Leu Phe 
                485                 490                 495     


Pro Arg Leu Glu Glu Met Gly Ala Arg Met Leu Leu Gln Val His Asp 
            500                 505                 510         


Glu Leu Val Leu Glu Ala Pro Lys Glu Arg Ala Glu Ala Val Ala Arg 
        515                 520                 525             


Leu Ala Lys Glu Val Met Glu Gly Val Tyr Pro Leu Ala Val Pro Leu 
    530                 535                 540                 


Glu Val Glu Val Gly Ile Gly Glu Asp Trp Leu Ser Ala Lys Glu 
545                 550                 555                 


<210>  14
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  small peptide fragment of split NanoLuc luciferase

<400>  14

Met Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser 
1               5                   10          


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  small peptide fragment of split NanoLuc luciferase

<400>  15

Met Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
1               5                   10          


<210>  16
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  small peptide fragment of split NanoLuc luciferase

<400>  16

Met Val Thr Gly Tyr Arg Leu Phe Glu Lys Ile Ser 
1               5                   10          


<210>  17
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  small peptide fragment of split NanoLuc luciferase

<400>  17

Met Asn Val Thr Gly Tyr Arg Leu Phe Lys Lys Ile Ser 
1               5                   10              


<210>  18
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  small peptide fragment of split NanoLuc luciferase

<400>  18

Met Asn Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser 
1               5                   10              


<210>  19
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  small peptide fragment of split NanoLuc luciferase

<400>  19

Met Gly Val Thr Gly Trp Arg Leu Cys Glu Arg Ile Leu Ala 
1               5                   10                  


<210>  20
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  engineered peptide

<400>  20

Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro 
1               5                   10          


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  engineered peptide


<220>
<221>  MISC_FEATURE
<222>  (4)..(7)
<223>  Xaa may be any amino acid

<400>  21

Gly Ala His Xaa Xaa Xaa Xaa Asp Ala Tyr Lys Pro 
1               5                   10          


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  engineered peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  Xaa may be any amino acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(11)
<223>  Xaa may be any amino acid

<400>  22

Xaa Xaa Xaa Ile Val Met Val Asp Xaa Xaa Xaa Pro 
1               5                   10          


<210>  23
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  engineered protein

<400>  23

Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
1               5                   10                  15      


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
            20                  25                  30          


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
        35                  40                  45              


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
    50                  55                  60                  


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
65                  70                  75                  80  


Val Asn Gly 
            


<210>  24
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  biotinylated peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  biotinylated

<400>  24

Ser Gly Ser Gly Gly Ala His Ile Val Met Val Asp Ala Tyr Lys Pro 
1               5                   10                  15      


Thr Lys 
        


<210>  25
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  biotinylated peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  biotinylated

<400>  25

Ser Gly Ser Gly Ser Phe Asp Ile Val Met Val Asp His Val Ser Pro 
1               5                   10                  15      


Thr Lys 
        


<210>  26
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pirmer

<400>  26
aaggagatat acatatgcgc ggcatgctgc cact                                   34


<210>  27
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  27
ggtggtggtg ctcgagtcat tccttggcac tcagccaatc ttc                         43


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  28
catatgtata tctccttctt aaagttaaac                                        30


<210>  29
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  29
agcccaaaag cgctg                                                        15


<210>  30
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  30
aaggagatat acatatgaag tcgggccgtc aggag                                  35


<210>  31
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  31
gcttttgggc tcatacctcc gcctacgtcg taggcaccgc gaagcttacg tctgatg          57


<210>  32
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  32
gccccgtggc cacctc                                                       16


<210>  33
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  33
ttcgcctcca cctgtttccg gcagtgggct ttccaagagc ccgaac                      46


<210>  34
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  34
ggagatatac atatggcaac cgttaaattc aagtataagg                             40


<210>  35
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  35
acctgtttcc ggcagactac cttttttttg cttttccagc a                           41


<210>  36
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  36
ctgccggaaa caggtggag                                                    19


<210>  37
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  37
aaggagatat acatatggga ggcgaagccc cg                                     32


<210>  38
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  38
tttctttacc agactcgagt cattccttgg cac                                    33


<210>  39
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  39
accacagcca ggatccagca accgttaaat tcaagtataa gg                          42


<210>  40
<211>  82
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  40
ccgcaagctt gtcgacttat cctccagtct caggcaggct cccaccacca ctaccttttt       60

tttgcttttc cagcatttgc ag                                                82


<210>  41
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  41
ataatgctta agtcgaacag aaagtaatcg tattg                                  35


<210>  42
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  42
tcctccagtc tcaggcagta cgt                                               23


<210>  43
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  43
cctgagactg gaggatctgt cgacgattca gccacccaca ttaaatttag taaacgcga        59


<210>  44
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  44
cgacttaagc attatgaatt cttagccgtt aactgtgacc tgtccctgtt catt             54


<210>  45
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  45
aaggagatat acatatggga gctcacatcg tgatggtgga cgcatataag ccgactaagg       60

gatctactag ttctatggga ggcgaagc                                          88


<210>  46
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  46
gcttcgcctc ccatagaact agtagatccc ttagtcggct tatatgcgtc caccatcacg       60

atgtgagctc ccatatgtat atctcctt                                          88


<210>  47
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  47
cctgagactg gaggatctgt cgacatggtc ttcacactcg aagatttcgt tgg              53


<210>  48
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  48
cgacttaagc attatgaatt cttaactgtt gatggttact cggaacagca tg               52


<210>  49
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  49
aaggagatat acatatggta accggttacc gtttgttcga agagattttg ggatctacta       60

gttctatggg aggcgaagc                                                    79


<210>  50
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  50
gcttcgcctc ccatagaact agtagatccc aaaatctctt cgaacaaacg gtaaccggtt       60

accatatgta tatctcctt                                                    79


<210>  51
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  51
aaggagatat acatatggta tcaggttggc gtttgttcaa gaagattagc ggatctacta       60

gttctatggg aggcgaagc                                                    79


<210>  52
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  52
gcttcgcctc ccatagaact agtagatccg ctaatcttct tgaacaaacg ccaacctgat       60

accatatgta tatctcctt                                                    79


<210>  53
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  53
aaggagatat acatatggta accggttacc gtttgttcga aaagattagc ggatctacta       60

gttctatggg aggcgaagc                                                    79


<210>  54
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  54
gcttcgcctc ccatagaact agtagatccg ctaatctttt cgaacaaacg gtaaccggtt       60

accatatgta tatctcctt                                                    79


<210>  55
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  55
aaggagatat acatatgaac gtaaccggtt accgtttgtt caagaagatt agcaacggat       60

ctactagttc tatgggaggc gaagc                                             85


<210>  56
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  56
gcttcgcctc ccatagaact agtagatccg ttgctaatct tcttgaacaa acggtaaccg       60

gttacgttca tatgtatatc tcctt                                             85


<210>  57
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  57
aaggagatat acatatgaac gtatcaggtt ggcgtttgtt caagaagatt agcaacggat       60

ctactagttc tatgggaggc gaagc                                             85


<210>  58
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  58
gcttcgcctc ccatagaact agtagatccg ttgctaatct tcttgaacaa acgccaacct       60

gatacgttca tatgtatatc tcctt                                             85


<210>  59
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  59
aaggagatat acatatgggt gtaaccggtt ggcgtttgtg cgaacgtatt ttggccggat       60

ctactagttc tatgggaggc gaagc                                             85


<210>  60
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  60
gcttcgcctc ccatagaact agtagatccg gccaaaatac gttcgcacaa acgccaaccg       60

gttacaccca tatgtatatc tcctt                                             85


<210>  61
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  61
aaggagatat acatatggga ggatctacta gttctatggg aggcgaagc                   49


<210>  62
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  62
gcttcgcctc ccatagaact agtagatcct cccatatgta tatctcctt                   49


<210>  63
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  63
tgcctgagac tggaggatct gtcgac                                            26


<210>  64
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (41)..(42)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (47)..(48)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (50)..(51)
<223>  n is a, c, g, or t

<400>  64
catagaacta gtagatccct tagtcggctt atatgcgtcm nnmnnmnnmn ngtgagctcc       60

catatgtata tctccttctt atacttaact aatatac                                97


<210>  65
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (29)..(30)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (32)..(33)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (35)..(36)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (53)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (56)..(57)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  n is a, c, g, or t

<400>  65
catagaacta gtagatccct tagtcggmnn mnnmnngtcc accatcacga tmnnmnnmnn       60

catatgtata tctccttctt atacttaact aatatac                                97


<210>  66
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (42)..(43)
<223>  n is a, c, g, or t

<400>  66
cagtagccac ttcatacccg tccggtgcgg cggtttccac mnnggtgtat ttacctgggt       60

acaggt                                                                  66


<210>  67
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<400>  67
gacgggtatg aagtggctac tgcaattact nnkccgtaaa tgaacaggga caggtcacag       60


<210>  68
<211>  97
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (50)..(51)
<223>  n is a, c, g, or t

<400>  68
catagaacta gtagatccct tagtcggctt atatgcgtcc accatcacmn ngtgagctcc       60

catatgtata tctccttctt atacttaact aatatac                                97


<210>  69
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  69
aaggagatat acatatgggc agcagccatc accatcatca cc                          42


<210>  70
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  70
gacggagctc gaattcttag ccgttaactg tgacctgtcc ctgttcattt ac               52


<210>  71
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  71
aaggagatat acatatggga gctcacatcg tgatggtgga cgc                         43


<210>  72
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  72
ggtggtggtg ctcgagttcc ttggcactca gccaatcttc gcc                         43


<210>  73
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotinylated

<400>  73
gtaagctgga agttgttgct gcgtgagcgg ataacaattc cccatcttag                  50


<210>  74
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  74
ttcgcctccc atagaactag tagatcc                                           27


<210>  75
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotinylated

<400>  75
gtaagctgga agttgttgct gctgcctgag actggaggat ctgtcgac                    48


<210>  76
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  76
gcctcccata gaactagtag atcccttagt c                                      31


<210>  77
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  77
gaagttgttg ctgcgtgagc ggat                                              24


<210>  78
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  78
gaagttgttg ctgctgcctg agac                                              24


<210>  79
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  79
tcccatagaa ctagtagatc ccttagtcgg                                        30


<210>  80
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  80
caagcagaag acggcatacg agatgccaat gtgactggag ttcagacgtg tgctcttccg       60

atctagctgt atattagtta agtataagaa ggagatatac atatg                      105


<210>  81
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  81
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctag       60

ctcatagaac tagtagatcc cttagtcgg                                         89


<210>  82
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  82
caagcagaag acggcatacg agatgccaat gtgactggag ttcagacgtg tgctcttccg       60

atctacgtga tatacatatg ggagctcac                                         89


<210>  83
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  83
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatctac       60

gtagatccct tagtcggctt atatgcgtc                                         89


<210>  84
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  84
catcggtgat gtcggcgat                                                    19


<210>  85
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  85
cggatatagt tcctcctttc agca                                              24


<210>  86
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif

<400>  86

Leu Val Leu Trp 
1               


<210>  87
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif

<400>  87

Met Met Leu Met 
1               


<210>  88
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif

<400>  88

Phe Val Phe Tyr 
1               


<210>  89
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif

<400>  89

Val Val Cys Arg 
1               


<210>  90
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif

<400>  90

Ile Val Met Val 
1               


<210>  91
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  motif

<400>  91

Ile Val Met Val Asp 
1               5   


<210>  92
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  engineered peptide

<400>  92

Ser Phe Asp Ile Val Met Val Asp His Val Ser Pro 
1               5                   10          


<210>  93
<211>  301
<212>  PRT
<213>  Methanopyrus kandleri

<400>  93

Met Lys Ser Gly Arg Gln Glu Arg Ser Glu Glu Glu Trp Lys Glu Trp 
1               5                   10                  15      


Leu Glu Arg Lys Val Gly Glu Gly Arg Ala Arg Arg Leu Ile Glu Tyr 
            20                  25                  30          


Phe Gly Ser Ala Gly Glu Val Gly Lys Leu Val Glu Asn Ala Glu Val 
        35                  40                  45              


Ser Lys Leu Leu Glu Val Pro Gly Ile Gly Asp Glu Ala Val Ala Arg 
    50                  55                  60                  


Leu Val Pro Gly Tyr Lys Thr Leu Arg Asp Ala Gly Leu Thr Pro Ala 
65                  70                  75                  80  


Glu Ala Glu Arg Val Leu Lys Arg Tyr Gly Ser Val Ser Lys Val Gln 
                85                  90                  95      


Glu Gly Ala Thr Pro Asp Glu Leu Arg Glu Leu Gly Leu Gly Asp Ala 
            100                 105                 110         


Lys Ile Ala Arg Ile Leu Gly Leu Arg Ser Leu Val Asn Lys Arg Leu 
        115                 120                 125             


Asp Val Asp Thr Ala Tyr Glu Leu Lys Arg Arg Tyr Gly Ser Val Ser 
    130                 135                 140                 


Ala Val Arg Lys Ala Pro Val Lys Glu Leu Arg Glu Leu Gly Leu Ser 
145                 150                 155                 160 


Asp Arg Lys Ile Ala Arg Ile Lys Gly Ile Pro Glu Thr Met Leu Gln 
                165                 170                 175     


Val Arg Gly Met Ser Val Glu Lys Ala Glu Arg Leu Leu Glu Arg Phe 
            180                 185                 190         


Asp Thr Trp Thr Lys Val Lys Glu Ala Pro Val Ser Glu Leu Val Arg 
        195                 200                 205             


Val Pro Gly Val Gly Leu Ser Leu Val Lys Glu Ile Lys Ala Gln Val 
    210                 215                 220                 


Asp Pro Ala Trp Lys Ala Leu Leu Asp Val Lys Gly Val Ser Pro Glu 
225                 230                 235                 240 


Leu Ala Asp Arg Leu Val Glu Glu Leu Gly Ser Pro Tyr Arg Val Leu 
                245                 250                 255     


Thr Ala Lys Lys Ser Asp Leu Met Arg Val Glu Arg Val Gly Pro Lys 
            260                 265                 270         


Leu Ala Glu Arg Ile Arg Ala Ala Gly Lys Arg Tyr Val Glu Glu Arg 
        275                 280                 285             


Arg Ser Arg Arg Glu Arg Ile Arg Arg Lys Leu Arg Gly 
    290                 295                 300     


<210>  94
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  engineered protein

<400>  94

Met Val Phe Thr Leu Glu Asp Phe Val Gly Asp Trp Glu Gln Thr Ala 
1               5                   10                  15      


Ala Tyr Asn Leu Asp Gln Val Leu Glu Gln Gly Gly Val Ser Ser Leu 
            20                  25                  30          


Leu Gln Asn Leu Ala Val Ser Val Thr Pro Ile Gln Arg Ile Val Arg 
        35                  40                  45              


Ser Gly Glu Asn Ala Leu Lys Ile Asp Ile His Val Ile Ile Pro Tyr 
    50                  55                  60                  


Glu Gly Leu Ser Ala Asp Gln Met Ala Gln Ile Glu Glu Val Phe Lys 
65                  70                  75                  80  


Val Val Tyr Pro Val Asp Asp His His Phe Lys Val Ile Leu Pro Tyr 
                85                  90                  95      


Gly Thr Leu Val Ile Asp Gly Val Thr Pro Asn Met Leu Asn Tyr Phe 
            100                 105                 110         


Gly Arg Pro Tyr Glu Gly Ile Ala Val Phe Asp Gly Lys Lys Ile Thr 
        115                 120                 125             


Val Thr Gly Thr Leu Trp Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu 
    130                 135                 140                 


Ile Thr Pro Asp Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
145                 150                 155                 


