                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  RECEPTOR

<130>  P118391PCT

<150>  GB 1911187.1
<151>  2019-08-05

<160>  71    

<170>  PatentIn version 3.5

<210>  1
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-2 receptor common gamma chain

<400>  1

Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu 
1               5                   10                  15      


Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys 
            20                  25                  30          


Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu 
        35                  40                  45              


Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly 
    50                  55                  60                  


Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr 
65                  70                  75                  80  


Thr Leu Lys Pro Glu Thr 
                85      


<210>  2
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-2 receptor beta chain 
       (IL-2Rbeta)

<400>  2

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
                245                 250                 255     


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
            260                 265                 270         


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        275                 280                 285     


<210>  3
<211>  195
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-7Ralpha

<400>  3

Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro Asp His Lys 
1               5                   10                  15      


Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn Leu Asn Val 
            20                  25                  30          


Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His Arg Val Asp 
        35                  40                  45              


Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln Asp Thr Phe 
    50                  55                  60                  


Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly Gly Asp Val 
65                  70                  75                  80  


Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr Pro Glu Ser 
                85                  90                  95      


Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn Val Ser Ala 
            100                 105                 110         


Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp Cys Arg Glu 
        115                 120                 125             


Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu Leu Ser Leu 
    130                 135                 140                 


Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu Gln Ser Gly 
145                 150                 155                 160 


Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile Leu Thr Ser 
                165                 170                 175     


Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser Ser Phe Tyr 
            180                 185                 190         


Gln Asn Gln 
        195 


<210>  4
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, hinge-CH2CH3 of human IgG1

<400>  4

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  5
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human CD8 stalk

<400>  5

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  6
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer sequence, human IgG1 hinge

<400>  6

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  7
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human common gamma chain

<400>  7

Val Val Ile Ser Val Gly Ser Met Gly Leu Ile Ile Ser Leu Leu Cys 
1               5                   10                  15      


Val Tyr Phe Trp Leu 
            20      


<210>  8
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human IL-2Rbeta

<400>  8

Ile Pro Trp Leu Gly His Leu Leu Val Gly Leu Ser Gly Ala Phe Gly 
1               5                   10                  15      


Phe Ile Ile Leu Val Tyr Leu Leu Ile 
            20                  25  


<210>  9
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human IL-7Ralpha

<400>  9

Pro Ile Leu Leu Thr Ile Ser Ile Leu Ser Phe Phe Ser Val Ala Leu 
1               5                   10                  15      


Leu Val Ile Leu Ala Cys Val Leu Trp 
            20                  25  


<210>  10
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human IL-15Ralpha

<400>  10

Ala Ile Ser Thr Ser Thr Val Leu Leu Cys Gly Leu Ser Ala Val Ser 
1               5                   10                  15      


Leu Leu Ala Cys Tyr Leu 
            20          


<210>  11
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA-binding mAb 5D3D11 heavy chain variable domain (VH)

<400>  11

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Ala Ile Ser Ser Ser 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Lys Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Val Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Gly Tyr Arg Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser 
        115             


<210>  12
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA-binding mAb 5D3D11 light chain variable domain (VL)

<400>  12

Asp Ile Val Met Thr Gln Thr Ala Pro Ser Val Phe Val Thr Pro Gly 
1               5                   10                  15      


Glu Ser Val Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Arg Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln His 
                85                  90                  95      


Leu Glu Tyr Pro Val Thr Phe Gly Ala Gly Thr Lys Val Glu Ile Lys 
            100                 105                 110         


<210>  13
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA-binding mAb 5D5A5 VH

<400>  13

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Ser Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Thr Gly Tyr Thr Glu Asn Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Val Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Arg Leu Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  14
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PSA-binding mAb 5D5A5 VL

<400>  14

Asp Ile Val Leu Thr Gln Ser Pro Pro Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Ser Ile Asp Leu Tyr 
            20                  25                  30          


Gly Phe Thr Phe Met His Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 
        35                  40                  45              


Lys Ile Leu Ile Tyr Arg Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asn 
65                  70                  75                  80  


Pro Val Glu Ala Asp Asp Val Ala Thr Tyr Tyr Cys Gln Gln Thr His 
                85                  90                  95      


Glu Asp Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110     


<210>  15
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  single-chain variable fragment (scFv) based on 5D5A5

<400>  15

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Ser Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Thr Gly Tyr Thr Glu Asn Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Val Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Gly Arg Leu Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser 
    130                 135                 140                 


Pro Pro Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 
145                 150                 155                 160 


Arg Ala Ser Glu Ser Ile Asp Leu Tyr Gly Phe Thr Phe Met His Trp 
                165                 170                 175     


Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Ile Leu Ile Tyr Arg Ala 
            180                 185                 190         


Ser Asn Leu Glu Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Arg Thr Asp Phe Thr Leu Thr Ile Asn Pro Val Glu Ala Asp Asp Val 
    210                 215                 220                 


Ala Thr Tyr Tyr Cys Gln Gln Thr His Glu Asp Pro Tyr Thr Phe Gly 
225                 230                 235                 240 


Gly Gly Thr Lys Leu Glu Ile Lys 
                245             


<210>  16
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv based on 5D3D11

<400>  16

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Ala Ile Ser Ser Ser 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Lys Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Val Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Gly Tyr Arg Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln 
    130                 135                 140                 


Thr Ala Pro Ser Val Phe Val Thr Pro Gly Glu Ser Val Ser Ile Ser 
145                 150                 155                 160 


Cys Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu 
                165                 170                 175     


Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr 
            180                 185                 190         


Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Arg Ile Ser Arg Val Glu Ala Glu 
    210                 215                 220                 


Asp Val Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr Pro Val Thr 
225                 230                 235                 240 


Phe Gly Ala Gly Thr Lys Val Glu Ile Lys 
                245                 250 


<210>  17
<211>  356
<212>  PRT
<213>  Homo sapiens

<400>  17

Met Ser Ile Pro Leu Pro Leu Leu Gln Ile Tyr Thr Ser Asp Asn Tyr 
1               5                   10                  15      


Thr Glu Glu Met Gly Ser Gly Asp Tyr Asp Ser Met Lys Glu Pro Cys 
            20                  25                  30          


Phe Arg Glu Glu Asn Ala Asn Phe Asn Lys Ile Phe Leu Pro Thr Ile 
        35                  40                  45              


Tyr Ser Ile Ile Phe Leu Thr Gly Ile Val Gly Asn Gly Leu Val Ile 
    50                  55                  60                  


Leu Val Met Gly Tyr Gln Lys Lys Leu Arg Ser Met Thr Asp Lys Tyr 
65                  70                  75                  80  


Arg Leu His Leu Ser Val Ala Asp Leu Leu Phe Val Ile Thr Leu Pro 
                85                  90                  95      


Phe Trp Ala Val Asp Ala Val Ala Asn Trp Tyr Phe Gly Asn Phe Leu 
            100                 105                 110         


Cys Lys Ala Val His Val Ile Tyr Thr Val Asn Leu Tyr Ser Ser Val 
        115                 120                 125             


Leu Ile Leu Ala Phe Ile Ser Leu Asp Arg Tyr Leu Ala Ile Val His 
    130                 135                 140                 


Ala Thr Asn Ser Gln Arg Pro Arg Lys Leu Leu Ala Glu Lys Val Val 
145                 150                 155                 160 


Tyr Val Gly Val Trp Ile Pro Ala Leu Leu Leu Thr Ile Pro Asp Phe 
                165                 170                 175     


Ile Phe Ala Asn Val Ser Glu Ala Asp Asp Arg Tyr Ile Cys Asp Arg 
            180                 185                 190         


Phe Tyr Pro Asn Asp Leu Trp Val Val Val Phe Gln Phe Gln His Ile 
        195                 200                 205             


Met Val Gly Leu Ile Leu Pro Gly Ile Val Ile Leu Ser Cys Tyr Cys 
    210                 215                 220                 


Ile Ile Ile Ser Lys Leu Ser His Ser Lys Gly His Gln Lys Arg Lys 
225                 230                 235                 240 


Ala Leu Lys Thr Thr Val Ile Leu Ile Leu Ala Phe Phe Ala Cys Trp 
                245                 250                 255     


Leu Pro Tyr Tyr Ile Gly Ile Ser Ile Asp Ser Phe Ile Leu Leu Glu 
            260                 265                 270         


Ile Ile Lys Gln Gly Cys Glu Phe Glu Asn Thr Val His Lys Trp Ile 
        275                 280                 285             


Ser Ile Thr Glu Ala Leu Ala Phe Phe His Cys Cys Leu Asn Pro Ile 
    290                 295                 300                 


Leu Tyr Ala Phe Leu Gly Ala Lys Phe Lys Thr Ser Ala Gln His Ala 
305                 310                 315                 320 


Leu Thr Ser Val Ser Arg Gly Ser Ser Leu Lys Ile Leu Ser Lys Gly 
                325                 330                 335     


Lys Arg Gly Gly His Ser Ser Val Ser Thr Glu Ser Glu Ser Ser Ser 
            340                 345                 350         


Phe His Ser Ser 
        355     


<210>  18
<211>  374
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Leu Ser Thr Ser Arg Ser Arg Phe Ile Arg Asn Thr Asn Glu Ser 
1               5                   10                  15      


Gly Glu Glu Val Thr Thr Phe Phe Asp Tyr Asp Tyr Gly Ala Pro Cys 
            20                  25                  30          


His Lys Phe Asp Val Lys Gln Ile Gly Ala Gln Leu Leu Pro Pro Leu 
        35                  40                  45              


Tyr Ser Leu Val Phe Ile Phe Gly Phe Val Gly Asn Met Leu Val Val 
    50                  55                  60                  


Leu Ile Leu Ile Asn Cys Lys Lys Leu Lys Cys Leu Thr Asp Ile Tyr 
65                  70                  75                  80  


Leu Leu Asn Leu Ala Ile Ser Asp Leu Leu Phe Leu Ile Thr Leu Pro 
                85                  90                  95      


Leu Trp Ala His Ser Ala Ala Asn Glu Trp Val Phe Gly Asn Ala Met 
            100                 105                 110         


Cys Lys Leu Phe Thr Gly Leu Tyr His Ile Gly Tyr Phe Gly Gly Ile 
        115                 120                 125             


Phe Phe Ile Ile Leu Leu Thr Ile Asp Arg Tyr Leu Ala Ile Val His 
    130                 135                 140                 


Ala Val Phe Ala Leu Lys Ala Arg Thr Val Thr Phe Gly Val Val Thr 
145                 150                 155                 160 


Ser Val Ile Thr Trp Leu Val Ala Val Phe Ala Ser Val Pro Gly Ile 
                165                 170                 175     


Ile Phe Thr Lys Cys Gln Lys Glu Asp Ser Val Tyr Val Cys Gly Pro 
            180                 185                 190         


Tyr Phe Pro Arg Gly Trp Asn Asn Phe His Thr Ile Met Arg Asn Ile 
        195                 200                 205             


Leu Gly Leu Val Leu Pro Leu Leu Ile Met Val Ile Cys Tyr Ser Gly 
    210                 215                 220                 


Ile Leu Lys Thr Leu Leu Arg Cys Arg Asn Glu Lys Lys Arg His Arg 
225                 230                 235                 240 


Ala Val Arg Val Ile Phe Thr Ile Met Ile Val Tyr Phe Leu Phe Trp 
                245                 250                 255     


Thr Pro Tyr Asn Ile Val Ile Leu Leu Asn Thr Phe Gln Glu Phe Phe 
            260                 265                 270         


Gly Leu Ser Asn Cys Glu Ser Thr Ser Gln Leu Asp Gln Ala Thr Gln 
        275                 280                 285             


Val Thr Glu Thr Leu Gly Met Thr His Cys Cys Ile Asn Pro Ile Ile 
    290                 295                 300                 


Tyr Ala Phe Val Gly Glu Lys Phe Arg Ser Leu Phe His Ile Ala Leu 
305                 310                 315                 320 


Gly Cys Arg Ile Ala Pro Leu Gln Lys Pro Val Cys Gly Gly Pro Gly 
                325                 330                 335     


Val Arg Pro Gly Lys Asn Val Lys Val Thr Thr Gln Gly Leu Leu Asp 
            340                 345                 350         


Gly Arg Gly Lys Gly Lys Ser Ile Gly Arg Ala Pro Glu Ala Ser Leu 
        355                 360                 365             


Gln Asp Lys Glu Gly Ala 
    370                 


<210>  19
<211>  360
<212>  PRT
<213>  Homo sapiens

<400>  19

Met Asn Pro Thr Asp Ile Ala Asp Thr Thr Leu Asp Glu Ser Ile Tyr 
1               5                   10                  15      


Ser Asn Tyr Tyr Leu Tyr Glu Ser Ile Pro Lys Pro Cys Thr Lys Glu 
            20                  25                  30          


Gly Ile Lys Ala Phe Gly Glu Leu Phe Leu Pro Pro Leu Tyr Ser Leu 
        35                  40                  45              


Val Phe Val Phe Gly Leu Leu Gly Asn Ser Val Val Val Leu Val Leu 
    50                  55                  60                  


Phe Lys Tyr Lys Arg Leu Arg Ser Met Thr Asp Val Tyr Leu Leu Asn 
65                  70                  75                  80  


Leu Ala Ile Ser Asp Leu Leu Phe Val Phe Ser Leu Pro Phe Trp Gly 
                85                  90                  95      


Tyr Tyr Ala Ala Asp Gln Trp Val Phe Gly Leu Gly Leu Cys Lys Met 
            100                 105                 110         


Ile Ser Trp Met Tyr Leu Val Gly Phe Tyr Ser Gly Ile Phe Phe Val 
        115                 120                 125             


Met Leu Met Ser Ile Asp Arg Tyr Leu Ala Ile Val His Ala Val Phe 
    130                 135                 140                 


Ser Leu Arg Ala Arg Thr Leu Thr Tyr Gly Val Ile Thr Ser Leu Ala 
145                 150                 155                 160 


Thr Trp Ser Val Ala Val Phe Ala Ser Leu Pro Gly Phe Leu Phe Ser 
                165                 170                 175     


Thr Cys Tyr Thr Glu Arg Asn His Thr Tyr Cys Lys Thr Lys Tyr Ser 
            180                 185                 190         


Leu Asn Ser Thr Thr Trp Lys Val Leu Ser Ser Leu Glu Ile Asn Ile 
        195                 200                 205             


Leu Gly Leu Val Ile Pro Leu Gly Ile Met Leu Phe Cys Tyr Ser Met 
    210                 215                 220                 


Ile Ile Arg Thr Leu Gln His Cys Lys Asn Glu Lys Lys Asn Lys Ala 
225                 230                 235                 240 


Val Lys Met Ile Phe Ala Val Val Val Leu Phe Leu Gly Phe Trp Thr 
                245                 250                 255     


Pro Tyr Asn Ile Val Leu Phe Leu Glu Thr Leu Val Glu Leu Glu Val 
            260                 265                 270         


Leu Gln Asp Cys Thr Phe Glu Arg Tyr Leu Asp Tyr Ala Ile Gln Ala 
        275                 280                 285             


Thr Glu Thr Leu Ala Phe Val His Cys Cys Leu Asn Pro Ile Ile Tyr 
    290                 295                 300                 


Phe Phe Leu Gly Glu Lys Phe Arg Lys Tyr Ile Leu Gln Leu Phe Lys 
305                 310                 315                 320 


Thr Cys Arg Gly Leu Phe Val Leu Cys Gln Tyr Cys Gly Leu Leu Gln 
                325                 330                 335     


Ile Tyr Ser Ala Asp Thr Pro Ser Ser Ser Tyr Thr Gln Ser Thr Met 
            340                 345                 350         


Asp His Asp Leu His Asp Ala Leu 
        355                 360 


<210>  20
<211>  238
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  J591 single-chain variable fragment (scFv)

<400>  20

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Thr 
1               5                   10                  15      


Ser Val Arg Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe Thr Glu Tyr 
            20                  25                  30          


Thr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Glu Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr 
            100                 105                 110         


Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        115                 120                 125             


Gly Ser Asp Ile Val Met Thr Gln Ser His Lys Phe Met Ser Thr Ser 
    130                 135                 140                 


Val Gly Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln Asp Val Gly 
145                 150                 155                 160 


Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu 
                165                 170                 175     


Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe 
            180                 185                 190         


Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Thr Asn Val 
        195                 200                 205             


Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr Asn Ser Tyr 
    210                 215                 220                 


Pro Leu Thr Phe Gly Ala Gly Thr Met Leu Asp Leu Lys Arg 
225                 230                 235             


<210>  21
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from TCR beta chain

<400>  21

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Gly 
            20      


<210>  22
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from IgG1

<400>  22

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  23
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide derived from CD8a

<400>  23

Met Ala Val Pro Thr Gln Val Leu Gly Leu Leu Leu Leu Trp Leu Thr 
1               5                   10                  15      


Asp Ala Arg Cys 
            20  


<210>  24
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 Z endodomain

<400>  24

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  25
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 and CD3 Zeta endodomains

<400>  25

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala 
        35                  40                  45              


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    50                  55                  60                  


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
65                  70                  75                  80  


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                85                  90                  95      


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            100                 105                 110         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        115                 120                 125             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    130                 135                 140                 


His Met Gln Ala Leu Pro Pro Arg 
145                 150         


<210>  26
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28, OX40 and CD3 Zeta endodomains

<400>  26

Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
1               5                   10                  15      


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
            20                  25                  30          


Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp 
        35                  40                  45              


Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu 
    50                  55                  60                  


Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys Phe 
65                  70                  75                  80  


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
                85                  90                  95      


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
            100                 105                 110         


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
        115                 120                 125             


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
    130                 135                 140                 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
145                 150                 155                 160 


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
                165                 170                 175     


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            180                 185             


<210>  27
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ICOS endodomain

<400>  27

Cys Trp Leu Thr Lys Lys Lys Tyr Ser Ser Ser Val His Asp Pro Asn 
1               5                   10                  15      


Gly Glu Tyr Met Phe Met Arg Ala Val Asn Thr Ala Lys Lys Ser Arg 
            20                  25                  30          


Leu Thr Asp Val Thr Leu 
        35              


<210>  28
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD27 endodomain

<400>  28

Gln Arg Arg Lys Tyr Arg Ser Asn Lys Gly Glu Ser Pro Val Glu Pro 
1               5                   10                  15      


Ala Glu Pro Cys His Tyr Ser Cys Pro Arg Glu Glu Glu Gly Ser Thr 
            20                  25                  30          


Ile Pro Ile Gln Glu Asp Tyr Arg Lys Pro Glu Pro Ala Cys Ser Pro 
        35                  40                  45              


<210>  29
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BTLA endodomain

<400>  29

Arg Arg His Gln Gly Lys Gln Asn Glu Leu Ser Asp Thr Ala Gly Arg 
1               5                   10                  15      


Glu Ile Asn Leu Val Asp Ala His Leu Lys Ser Glu Gln Thr Glu Ala 
            20                  25                  30          


Ser Thr Arg Gln Asn Ser Gln Val Leu Leu Ser Glu Thr Gly Ile Tyr 
        35                  40                  45              


Asp Asn Asp Pro Asp Leu Cys Phe Arg Met Gln Glu Gly Ser Glu Val 
    50                  55                  60                  


Tyr Ser Asn Pro Cys Leu Glu Glu Asn Lys Pro Gly Ile Val Tyr Ala 
65                  70                  75                  80  


Ser Leu Asn His Ser Val Ile Gly Pro Asn Ser Arg Leu Ala Arg Asn 
                85                  90                  95      


Val Lys Glu Ala Pro Thr Glu Tyr Ala Ser Ile Cys Val Arg Ser 
            100                 105                 110     


<210>  30
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD30 endodomain

<400>  30

His Arg Arg Ala Cys Arg Lys Arg Ile Arg Gln Lys Leu His Leu Cys 
1               5                   10                  15      


Tyr Pro Val Gln Thr Ser Gln Pro Lys Leu Glu Leu Val Asp Ser Arg 
            20                  25                  30          


Pro Arg Arg Ser Ser Thr Gln Leu Arg Ser Gly Ala Ser Val Thr Glu 
        35                  40                  45              


Pro Val Ala Glu Glu Arg Gly Leu Met Ser Gln Pro Leu Met Glu Thr 
    50                  55                  60                  


Cys His Ser Val Gly Ala Ala Tyr Leu Glu Ser Leu Pro Leu Gln Asp 
65                  70                  75                  80  


Ala Ser Pro Ala Gly Gly Pro Ser Ser Pro Arg Asp Leu Pro Glu Pro 
                85                  90                  95      


Arg Val Ser Thr Glu His Thr Asn Asn Lys Ile Glu Lys Ile Tyr Ile 
            100                 105                 110         


Met Lys Ala Asp Thr Val Ile Val Gly Thr Val Lys Ala Glu Leu Pro 
        115                 120                 125             


Glu Gly Arg Gly Leu Ala Gly Pro Ala Glu Pro Glu Leu Glu Glu Glu 
    130                 135                 140                 


Leu Glu Ala Asp His Thr Pro His Tyr Pro Glu Gln Glu Thr Glu Pro 
145                 150                 155                 160 


Pro Leu Gly Ser Cys Ser Asp Val Met Leu Ser Val Glu Glu Glu Gly 
                165                 170                 175     


Lys Glu Asp Pro Leu Pro Thr Ala Ala Ser Gly Lys 
            180                 185             


<210>  31
<211>  58
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GITR endodomain

<400>  31

Gln Leu Gly Leu His Ile Trp Gln Leu Arg Ser Gln Cys Met Trp Pro 
1               5                   10                  15      


Arg Glu Thr Gln Leu Leu Leu Glu Val Pro Pro Ser Thr Glu Asp Ala 
            20                  25                  30          


Arg Ser Cys Gln Phe Pro Glu Glu Glu Arg Gly Glu Arg Ser Ala Glu 
        35                  40                  45              


Glu Lys Gly Arg Leu Gly Asp Leu Trp Val 
    50                  55              


<210>  32
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HVEM endodomain

<400>  32

Cys Val Lys Arg Arg Lys Pro Arg Gly Asp Val Val Lys Val Ile Val 
1               5                   10                  15      


Ser Val Gln Arg Lys Arg Gln Glu Ala Glu Gly Glu Ala Thr Val Ile 
            20                  25                  30          


Glu Ala Leu Gln Ala Pro Pro Asp Val Thr Thr Val Ala Val Glu Glu 
        35                  40                  45              


Thr Ile Pro Ser Phe Thr Gly Arg Ser Pro Asn His 
    50                  55                  60  


<210>  33
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  33

Tyr His Ala Asp Tyr Tyr Lys Gln Arg Leu Ile His Asp Val Glu Met 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  34
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  34

His Tyr Ala Gly Tyr Phe Ala Asp Leu Leu Ile His Asp Ile Glu Thr 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  35
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  35

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  36
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  36

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  37
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  37

Ala Ala Arg Gln Met Leu Leu Leu Leu Ser Gly Asp Val Glu Thr Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  38
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  38

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  39
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  39

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Gly Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  40
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  40

Thr Arg Ala Glu Ile Glu Asp Glu Leu Ile Arg Ala Asp Ile Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  41
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  41

Ala Lys Phe Gln Ile Asp Lys Ile Leu Ile Ser Gly Asp Val Glu Leu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  42
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  42

Ser Ser Ile Ile Arg Thr Lys Met Leu Val Ser Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  43
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  43

Cys Asp Ala Gln Arg Gln Lys Leu Leu Leu Ser Gly Asp Ile Glu Gln 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  44
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cleavage site, 2A-like sequence

<400>  44

Tyr Pro Ile Asp Phe Gly Gly Phe Leu Val Lys Ala Asp Ser Glu Phe 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  45
<211>  1722
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  illustrative construct with IL-2R beta chain

<400>  45

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Lys Lys 
            20                  25                  30          


Pro Gly Thr Ser Val Arg Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Glu Tyr Thr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu 
    50                  55                  60                  


Glu Trp Ile Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Glu Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser His Lys Phe Met 
145                 150                 155                 160 


Ser Thr Ser Val Gly Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln 
                165                 170                 175     


Asp Val Gly Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser 
            180                 185                 190         


Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro 
        195                 200                 205             


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
    210                 215                 220                 


Thr Asn Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr 
225                 230                 235                 240 


Asn Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Met Leu Asp Leu Lys 
                245                 250                 255     


Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe 
        275                 280                 285             


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val 
    290                 295                 300                 


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
305                 310                 315                 320 


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
                325                 330                 335     


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
            340                 345                 350         


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
        355                 360                 365             


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
    370                 375                 380                 


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
385                 390                 395                 400 


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
                405                 410                 415     


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
            420                 425                 430         


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
        435                 440                 445             


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
    450                 455                 460                 


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
465                 470                 475                 480 


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe Trp Val Leu 
                485                 490                 495     


Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val 
            500                 505                 510         


Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg Ser Ala 
        515                 520                 525             


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    530                 535                 540                 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
545                 550                 555                 560 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                565                 570                 575     


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            580                 585                 590         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        595                 600                 605             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    610                 615                 620                 


His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly Ser Leu 
625                 630                 635                 640 


Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Glu Thr Asp 
                645                 650                 655     


Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro Gly Ser Thr Gly 
            660                 665                 670         


Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
        675                 680                 685             


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Ser Phe Ser Ser Tyr 
    690                 695                 700                 


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
705                 710                 715                 720 


Gly Tyr Ile Asn Pro Ser Thr Gly Tyr Thr Glu Asn Asn Gln Lys Phe 
                725                 730                 735     


Lys Asp Lys Val Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
            740                 745                 750         


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
        755                 760                 765             


Ala Arg Ser Gly Arg Leu Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr 
    770                 775                 780                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
785                 790                 795                 800 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser 
                805                 810                 815     


Pro Pro Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 
            820                 825                 830         


Arg Ala Ser Glu Ser Ile Asp Leu Tyr Gly Phe Thr Phe Met His Trp 
        835                 840                 845             


Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Ile Leu Ile Tyr Arg Ala 
    850                 855                 860                 


Ser Asn Leu Glu Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser 
865                 870                 875                 880 


Arg Thr Asp Phe Thr Leu Thr Ile Asn Pro Val Glu Ala Asp Asp Val 
                885                 890                 895     


Ala Thr Tyr Tyr Cys Gln Gln Thr His Glu Asp Pro Tyr Thr Phe Gly 
            900                 905                 910         


Gly Gly Thr Lys Leu Glu Ile Lys Ser Asp Pro Ala Thr Thr Thr Pro 
        915                 920                 925             


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
    930                 935                 940                 


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
945                 950                 955                 960 


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Val Val Ile Ser Val Gly 
                965                 970                 975     


Ser Met Gly Leu Ile Ile Ser Leu Leu Cys Val Tyr Phe Trp Leu Glu 
            980                 985                 990         


Arg Thr Met Pro Arg Ile Pro Thr  Leu Lys Asn Leu Glu  Asp Leu Val 
        995                 1000                 1005             


Thr Glu  Tyr His Gly Asn Phe  Ser Ala Trp Ser Gly  Val Ser Lys 
    1010                 1015                 1020             


Gly Leu  Ala Glu Ser Leu Gln  Pro Asp Tyr Ser Glu  Arg Leu Cys 
    1025                 1030                 1035             


Leu Val  Ser Glu Ile Pro Pro  Lys Gly Gly Ala Leu  Gly Glu Gly 
    1040                 1045                 1050             


Pro Gly  Ala Ser Pro Cys Asn  Gln His Ser Pro Tyr  Trp Ala Pro 
    1055                 1060                 1065             


Pro Cys  Tyr Thr Leu Lys Pro  Glu Thr Gln Cys Thr  Asn Tyr Ala 
    1070                 1075                 1080             


Leu Leu  Lys Leu Ala Gly Asp  Val Glu Ser Asn Pro  Gly Pro Met 
    1085                 1090                 1095             


Glu Thr  Asp Thr Leu Ile Leu  Trp Val Leu Leu Leu  Leu Val Pro 
    1100                 1105                 1110             


Gly Ser  Thr Gly Gln Val Gln  Leu Gln Gln Ser Gly  Pro Glu Leu 
    1115                 1120                 1125             


Val Lys  Pro Gly Ala Ser Val  Lys Ile Ser Cys Lys  Val Ser Gly 
    1130                 1135                 1140             


Tyr Ala  Ile Ser Ser Ser Trp  Met Asn Trp Val Lys  Gln Arg Pro 
    1145                 1150                 1155             


Gly Gln  Gly Leu Glu Trp Ile  Gly Arg Ile Tyr Pro  Gly Asp Gly 
    1160                 1165                 1170             


Asp Thr  Lys Tyr Asn Gly Lys  Phe Lys Asp Lys Ala  Thr Leu Thr 
    1175                 1180                 1185             


Val Asp  Lys Ser Ser Ser Thr  Ala Tyr Met Gln Leu  Ser Ser Leu 
    1190                 1195                 1200             


Thr Ser  Val Asp Ser Ala Val  Tyr Phe Cys Ala Arg  Asp Gly Tyr 
    1205                 1210                 1215             


Arg Tyr  Tyr Phe Asp Tyr Trp  Gly Gln Gly Thr Ser  Val Thr Val 
    1220                 1225                 1230             


Ser Ser  Gly Gly Gly Gly Ser  Gly Gly Gly Gly Ser  Gly Gly Gly 
    1235                 1240                 1245             


Gly Ser  Gly Gly Gly Gly Ser  Asp Ile Val Met Thr  Gln Thr Ala 
    1250                 1255                 1260             


Pro Ser  Val Phe Val Thr Pro  Gly Glu Ser Val Ser  Ile Ser Cys 
    1265                 1270                 1275             


Arg Ser  Ser Lys Ser Leu Leu  His Ser Asn Gly Asn  Thr Tyr Leu 
    1280                 1285                 1290             


Tyr Trp  Phe Leu Gln Arg Pro  Gly Gln Ser Pro Gln  Leu Leu Ile 
    1295                 1300                 1305             


Tyr Arg  Met Ser Asn Leu Ala  Ser Gly Val Pro Asp  Arg Phe Ser 
    1310                 1315                 1320             


Gly Ser  Gly Ser Gly Thr Asp  Phe Thr Leu Arg Ile  Ser Arg Val 
    1325                 1330                 1335             


Glu Ala  Glu Asp Val Gly Val  Tyr Tyr Cys Met Gln  His Leu Glu 
    1340                 1345                 1350             


Tyr Pro  Val Thr Phe Gly Ala  Gly Thr Lys Val Glu  Ile Lys Ser 
    1355                 1360                 1365             


Asp Pro  Ala Lys Ile Glu Val  Met Tyr Pro Pro Pro  Tyr Leu Asp 
    1370                 1375                 1380             


Asn Glu  Lys Ser Asn Gly Thr  Ile Ile His Val Lys  Gly Lys His 
    1385                 1390                 1395             


Leu Cys  Pro Ser Pro Leu Phe  Pro Gly Pro Ser Lys  Pro Ile Pro 
    1400                 1405                 1410             


Trp Leu  Gly His Leu Leu Val  Gly Leu Ser Gly Ala  Phe Gly Phe 
    1415                 1420                 1425             


Ile Ile  Leu Val Tyr Leu Leu  Ile Asn Cys Arg Asn  Thr Gly Pro 
    1430                 1435                 1440             


Trp Leu  Lys Lys Val Leu Lys  Cys Asn Thr Pro Asp  Pro Ser Lys 
    1445                 1450                 1455             


Phe Phe  Ser Gln Leu Ser Ser  Glu His Gly Gly Asp  Val Gln Lys 
    1460                 1465                 1470             


Trp Leu  Ser Ser Pro Phe Pro  Ser Ser Ser Phe Ser  Pro Gly Gly 
    1475                 1480                 1485             


Leu Ala  Pro Glu Ile Ser Pro  Leu Glu Val Leu Glu  Arg Asp Lys 
    1490                 1495                 1500             


Val Thr  Gln Leu Leu Leu Gln  Gln Asp Lys Val Pro  Glu Pro Ala 
    1505                 1510                 1515             


Ser Leu  Ser Ser Asn His Ser  Leu Thr Ser Cys Phe  Thr Asn Gln 
    1520                 1525                 1530             


Gly Tyr  Phe Phe Phe His Leu  Pro Asp Ala Leu Glu  Ile Glu Ala 
    1535                 1540                 1545             


Cys Gln  Val Tyr Phe Thr Tyr  Asp Pro Tyr Ser Glu  Glu Asp Pro 
    1550                 1555                 1560             


Asp Glu  Gly Val Ala Gly Ala  Pro Thr Gly Ser Ser  Pro Gln Pro 
    1565                 1570                 1575             


Leu Gln  Pro Leu Ser Gly Glu  Asp Asp Ala Tyr Cys  Thr Phe Pro 
    1580                 1585                 1590             


Ser Arg  Asp Asp Leu Leu Leu  Phe Ser Pro Ser Leu  Leu Gly Gly 
    1595                 1600                 1605             


Pro Ser  Pro Pro Ser Thr Ala  Pro Gly Gly Ser Gly  Ala Gly Glu 
    1610                 1615                 1620             


Glu Arg  Met Pro Pro Ser Leu  Gln Glu Arg Val Pro  Arg Asp Trp 
    1625                 1630                 1635             


Asp Pro  Gln Pro Leu Gly Pro  Pro Thr Pro Gly Val  Pro Asp Leu 
    1640                 1645                 1650             


Val Asp  Phe Gln Pro Pro Pro  Glu Leu Val Leu Arg  Glu Ala Gly 
    1655                 1660                 1665             


Glu Glu  Val Pro Asp Ala Gly  Pro Arg Glu Gly Val  Ser Phe Pro 
    1670                 1675                 1680             


Trp Ser  Arg Pro Pro Gly Gln  Gly Glu Phe Arg Ala  Leu Asn Ala 
    1685                 1690                 1695             


Arg Leu  Pro Leu Asn Thr Asp  Ala Tyr Leu Ser Leu  Gln Glu Leu 
    1700                 1705                 1710             


Gln Gly  Gln Asp Pro Thr His  Leu Val 
    1715                 1720         


<210>  46
<211>  1631
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  illustrative construct with IL-7R alpha chain

<400>  46

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Lys Lys 
            20                  25                  30          


Pro Gly Thr Ser Val Arg Ile Ser Cys Lys Thr Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Glu Tyr Thr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu 
    50                  55                  60                  


Glu Trp Ile Gly Asn Ile Asn Pro Asn Asn Gly Gly Thr Thr Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Glu Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Gly Trp Asn Phe Asp Tyr Trp Gly Gln Gly Thr 
        115                 120                 125             


Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ser His Lys Phe Met 
145                 150                 155                 160 


Ser Thr Ser Val Gly Asp Arg Val Ser Ile Ile Cys Lys Ala Ser Gln 
                165                 170                 175     


Asp Val Gly Thr Ala Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ser 
            180                 185                 190         


Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro 
        195                 200                 205             


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
    210                 215                 220                 


Thr Asn Val Gln Ser Glu Asp Leu Ala Asp Tyr Phe Cys Gln Gln Tyr 
225                 230                 235                 240 


Asn Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Met Leu Asp Leu Lys 
                245                 250                 255     


Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys 
            260                 265                 270         


Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe 
        275                 280                 285             


Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val 
    290                 295                 300                 


Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
305                 310                 315                 320 


Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
                325                 330                 335     


Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
            340                 345                 350         


Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
        355                 360                 365             


Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
    370                 375                 380                 


Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg 
385                 390                 395                 400 


Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly 
                405                 410                 415     


Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro 
            420                 425                 430         


Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser 
        435                 440                 445             


Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln 
    450                 455                 460                 


Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His 
465                 470                 475                 480 


Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Phe Trp Val Leu 
                485                 490                 495     


Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val 
            500                 505                 510         


Ala Phe Ile Ile Phe Trp Val Arg Arg Val Lys Phe Ser Arg Ser Ala 
        515                 520                 525             


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
    530                 535                 540                 


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
545                 550                 555                 560 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
                565                 570                 575     


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
            580                 585                 590         


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
        595                 600                 605             


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
    610                 615                 620                 


His Met Gln Ala Leu Pro Pro Arg Arg Ala Glu Gly Arg Gly Ser Leu 
625                 630                 635                 640 


Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Glu Thr Asp 
                645                 650                 655     


Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro Gly Ser Thr Gly 
            660                 665                 670         


Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
        675                 680                 685             


Ser Val Lys Met Ser Cys Lys Thr Ser Gly Tyr Ser Phe Ser Ser Tyr 
    690                 695                 700                 


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
705                 710                 715                 720 


Gly Tyr Ile Asn Pro Ser Thr Gly Tyr Thr Glu Asn Asn Gln Lys Phe 
                725                 730                 735     


Lys Asp Lys Val Thr Leu Thr Ala Asp Lys Ser Ser Asn Thr Ala Tyr 
            740                 745                 750         


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
        755                 760                 765             


Ala Arg Ser Gly Arg Leu Tyr Phe Asp Val Trp Gly Ala Gly Thr Thr 
    770                 775                 780                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
785                 790                 795                 800 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Leu Thr Gln Ser 
                805                 810                 815     


Pro Pro Ser Leu Ala Val Ser Leu Gly Gln Arg Ala Thr Ile Ser Cys 
            820                 825                 830         


Arg Ala Ser Glu Ser Ile Asp Leu Tyr Gly Phe Thr Phe Met His Trp 
        835                 840                 845             


Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Ile Leu Ile Tyr Arg Ala 
    850                 855                 860                 


Ser Asn Leu Glu Ser Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser 
865                 870                 875                 880 


Arg Thr Asp Phe Thr Leu Thr Ile Asn Pro Val Glu Ala Asp Asp Val 
                885                 890                 895     


Ala Thr Tyr Tyr Cys Gln Gln Thr His Glu Asp Pro Tyr Thr Phe Gly 
            900                 905                 910         


Gly Gly Thr Lys Leu Glu Ile Lys Ser Asp Pro Ala Thr Thr Thr Pro 
        915                 920                 925             


Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu 
    930                 935                 940                 


Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His 
945                 950                 955                 960 


Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Val Val Ile Ser Val Gly 
                965                 970                 975     


Ser Met Gly Leu Ile Ile Ser Leu Leu Cys Val Tyr Phe Trp Leu Glu 
            980                 985                 990         


Arg Thr Met Pro Arg Ile Pro Thr  Leu Lys Asn Leu Glu  Asp Leu Val 
        995                 1000                 1005             


Thr Glu  Tyr His Gly Asn Phe  Ser Ala Trp Ser Gly  Val Ser Lys 
    1010                 1015                 1020             


Gly Leu  Ala Glu Ser Leu Gln  Pro Asp Tyr Ser Glu  Arg Leu Cys 
    1025                 1030                 1035             


Leu Val  Ser Glu Ile Pro Pro  Lys Gly Gly Ala Leu  Gly Glu Gly 
    1040                 1045                 1050             


Pro Gly  Ala Ser Pro Cys Asn  Gln His Ser Pro Tyr  Trp Ala Pro 
    1055                 1060                 1065             


Pro Cys  Tyr Thr Leu Lys Pro  Glu Thr Gln Cys Thr  Asn Tyr Ala 
    1070                 1075                 1080             


Leu Leu  Lys Leu Ala Gly Asp  Val Glu Ser Asn Pro  Gly Pro Met 
    1085                 1090                 1095             


Glu Thr  Asp Thr Leu Ile Leu  Trp Val Leu Leu Leu  Leu Val Pro 
    1100                 1105                 1110             


Gly Ser  Thr Gly Gln Val Gln  Leu Gln Gln Ser Gly  Pro Glu Leu 
    1115                 1120                 1125             


Val Lys  Pro Gly Ala Ser Val  Lys Ile Ser Cys Lys  Val Ser Gly 
    1130                 1135                 1140             


Tyr Ala  Ile Ser Ser Ser Trp  Met Asn Trp Val Lys  Gln Arg Pro 
    1145                 1150                 1155             


Gly Gln  Gly Leu Glu Trp Ile  Gly Arg Ile Tyr Pro  Gly Asp Gly 
    1160                 1165                 1170             


Asp Thr  Lys Tyr Asn Gly Lys  Phe Lys Asp Lys Ala  Thr Leu Thr 
    1175                 1180                 1185             


Val Asp  Lys Ser Ser Ser Thr  Ala Tyr Met Gln Leu  Ser Ser Leu 
    1190                 1195                 1200             


Thr Ser  Val Asp Ser Ala Val  Tyr Phe Cys Ala Arg  Asp Gly Tyr 
    1205                 1210                 1215             


Arg Tyr  Tyr Phe Asp Tyr Trp  Gly Gln Gly Thr Ser  Val Thr Val 
    1220                 1225                 1230             


Ser Ser  Gly Gly Gly Gly Ser  Gly Gly Gly Gly Ser  Gly Gly Gly 
    1235                 1240                 1245             


Gly Ser  Gly Gly Gly Gly Ser  Asp Ile Val Met Thr  Gln Thr Ala 
    1250                 1255                 1260             


Pro Ser  Val Phe Val Thr Pro  Gly Glu Ser Val Ser  Ile Ser Cys 
    1265                 1270                 1275             


Arg Ser  Ser Lys Ser Leu Leu  His Ser Asn Gly Asn  Thr Tyr Leu 
    1280                 1285                 1290             


Tyr Trp  Phe Leu Gln Arg Pro  Gly Gln Ser Pro Gln  Leu Leu Ile 
    1295                 1300                 1305             


Tyr Arg  Met Ser Asn Leu Ala  Ser Gly Val Pro Asp  Arg Phe Ser 
    1310                 1315                 1320             


Gly Ser  Gly Ser Gly Thr Asp  Phe Thr Leu Arg Ile  Ser Arg Val 
    1325                 1330                 1335             


Glu Ala  Glu Asp Val Gly Val  Tyr Tyr Cys Met Gln  His Leu Glu 
    1340                 1345                 1350             


Tyr Pro  Val Thr Phe Gly Ala  Gly Thr Lys Val Glu  Ile Lys Ser 
    1355                 1360                 1365             


Asp Pro  Ala Lys Ile Glu Val  Met Tyr Pro Pro Pro  Tyr Leu Asp 
    1370                 1375                 1380             


Asn Glu  Lys Ser Asn Gly Thr  Ile Ile His Val Lys  Gly Lys His 
    1385                 1390                 1395             


Leu Cys  Pro Ser Pro Leu Phe  Pro Gly Pro Ser Lys  Pro Pro Ile 
    1400                 1405                 1410             


Leu Leu  Thr Ile Ser Ile Leu  Ser Phe Phe Ser Val  Ala Leu Leu 
    1415                 1420                 1425             


Val Ile  Leu Ala Cys Val Leu  Trp Lys Lys Arg Ile  Lys Pro Ile 
    1430                 1435                 1440             


Val Trp  Pro Ser Leu Pro Asp  His Lys Lys Thr Leu  Glu His Leu 
    1445                 1450                 1455             


Cys Lys  Lys Pro Arg Lys Asn  Leu Asn Val Ser Phe  Asn Pro Glu 
    1460                 1465                 1470             


Ser Phe  Leu Asp Cys Gln Ile  His Arg Val Asp Asp  Ile Gln Ala 
    1475                 1480                 1485             


Arg Asp  Glu Val Glu Gly Phe  Leu Gln Asp Thr Phe  Pro Gln Gln 
    1490                 1495                 1500             


Leu Glu  Glu Ser Glu Lys Gln  Arg Leu Gly Gly Asp  Val Gln Ser 
    1505                 1510                 1515             


Pro Asn  Cys Pro Ser Glu Asp  Val Val Ile Thr Pro  Glu Ser Phe 
    1520                 1525                 1530             


Gly Arg  Asp Ser Ser Leu Thr  Cys Leu Ala Gly Asn  Val Ser Ala 
    1535                 1540                 1545             


Cys Asp  Ala Pro Ile Leu Ser  Ser Ser Arg Ser Leu  Asp Cys Arg 
    1550                 1555                 1560             


Glu Ser  Gly Lys Asn Gly Pro  His Val Tyr Gln Asp  Leu Leu Leu 
    1565                 1570                 1575             


Ser Leu  Gly Thr Thr Asn Ser  Thr Leu Pro Pro Pro  Phe Ser Leu 
    1580                 1585                 1590             


Gln Ser  Gly Ile Leu Thr Leu  Asn Pro Val Ala Gln  Gly Gln Pro 
    1595                 1600                 1605             


Ile Leu  Thr Ser Leu Gly Ser  Asn Gln Glu Glu Ala  Tyr Val Thr 
    1610                 1615                 1620             


Met Ser  Ser Phe Tyr Gln Asn  Gln 
    1625                 1630     


<210>  47
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-2Rbeta Box 1 motif

<400>  47

Lys Cys Asn Thr Pro Asp Pro Ser 
1               5               


<210>  48
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-2Rbeta Box 2 motif

<400>  48

Ser Pro Leu Glu Val Leu Glu Arg Asp Lys Val 
1               5                   10      


<210>  49
<211>  68
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-331

<400>  49

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val 
65              


<210>  50
<211>  86
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-351

<400>  50

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser 
                85      


<210>  51
<211>  105
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-371

<400>  51

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro 
            100                 105 


<210>  52
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-391

<400>  52

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp 
        115                 120                 125     


<210>  53
<211>  146
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-411

<400>  53

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu 
145     


<210>  54
<211>  166
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-431

<400>  54

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser 
                165     


<210>  55
<211>  186
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-451

<400>  55

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly 
            180                 185     


<210>  56
<211>  206
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-471

<400>  56

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro 
        195                 200                 205     


<210>  57
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-491

<400>  57

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val 
225         


<210>  58
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-511

<400>  58

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe 
                245     


<210>  59
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  truncated IL-2Rbeta aa266-531

<400>  59

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
                245                 250                 255     


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu 
            260                 265     


<210>  60
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from human IL-15Ralpha

<400>  60

Ser Arg Gln Thr Pro Pro Leu Ala Ser Val Glu Met Glu Ala Met Glu 
1               5                   10                  15      


Ala Leu Pro Val Thr Trp Gly Thr Ser Ser Arg Asp Glu Asp Leu Glu 
            20                  25                  30          


Asn Cys Ser His His Leu 
        35              


<210>  61
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  cytokine receptor motif


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid

<400>  61

Trp Ser Xaa Trp Ser 
1               5   


<210>  62
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic amino acid furin target sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Arg or Lys

<400>  62

Arg Xaa Xaa Arg 
1               


<210>  63
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  63

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  64
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal sequence derived from human CD8a

<400>  64

Met Ser Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala 
            


<210>  65
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  65

Ser Asp Pro Ala 
1               


<210>  66
<211>  231
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1Fc spacer (HCH2CH3pvaa)

<400>  66

Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
            20                  25                  30          


Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val 
        35                  40                  45              


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
    50                  55                  60                  


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
65                  70                  75                  80  


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
                85                  90                  95      


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
            100                 105                 110         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
        115                 120                 125             


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
    130                 135                 140                 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
145                 150                 155                 160 


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
                165                 170                 175     


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
            180                 185                 190         


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
        195                 200                 205             


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Leu Ser Leu Ser Pro Gly Lys 
225                 230     


<210>  67
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  transmembrane derived from human CD28

<400>  67

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  68
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  endodomain derived from TCRz

<400>  68

Arg Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
1               5                   10                  15      


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
            20                  25                  30          


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
        35                  40                  45              


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         


Arg 
    


<210>  69
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal sequence derived from mouse kappa VIII

<400>  69

Met Glu Thr Asp Thr Leu Ile Leu Trp Val Leu Leu Leu Leu Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  70
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv aPSA (5D3D11 H/L)

<400>  70

Gln Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Ala Ile Ser Ser Ser 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Tyr Pro Gly Asp Gly Asp Thr Lys Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Val Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Gly Tyr Arg Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln 
    130                 135                 140                 


Thr Ala Pro Ser Val Phe Val Thr Pro Gly Glu Ser Val Ser Ile Ser 
145                 150                 155                 160 


Cys Arg Ser Ser Lys Ser Leu Leu His Ser Asn Gly Asn Thr Tyr Leu 
                165                 170                 175     


Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr 
            180                 185                 190         


Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser 
        195                 200                 205             


Gly Ser Gly Thr Asp Phe Thr Leu Arg Ile Ser Arg Val Glu Ala Glu 
    210                 215                 220                 


Asp Val Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr Pro Val Thr 
225                 230                 235                 240 


Phe Gly Ala Gly Thr Lys Val Glu Ile Lys 
                245                 250 


<210>  71
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human CD28STK spacer

<400>  71

Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
1               5                   10                  15      


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
            20                  25                  30          


Leu Phe Pro Gly Pro Ser Lys Pro 
        35                  40  


