                         SEQUENCE LISTING

<110>  Kabushiki Kaisha Toshiba
       Shinshu University
 
<120>  METHOD OF PRODUCING CAR-T CELLS, NUCLEIC ACID-INTRODUCING CARRIER
       AND KIT

<130>  20S0109PCT

<150>  JP2019-135549
<151>  2019-07-23

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  4536
<212>  DNA
<213>  Artificial

<220>
<223>  5'IR_CAR gene_3'IR

<400>  1
tttaacccta gaaagatagt ctgcgtaaaa ttgacgcatg cattcttgaa atattgctct       60

ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg acatctcagt cgccgcttgg      120

agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac tgattttgaa ctataacgac      180

cgcgtgagtc aaaatgacgc atgattatct tttacgtgac ttttaagatt taactcatac      240

gataattata ttgttatttc atgttctact tacgtgataa cttattatat atatattttc      300

ttgttataga taagatcttc aatattggcc attagccata ttattcattg gttatatagc      360

ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat      420

ttatattggc tcatgtccaa tatgaccgcc atgttggcat tgattattga ctagttatta      480

atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata      540

acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat      600

aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga      660

gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtccgcc      720

ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt      780

acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat      840

gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg gatttccaag      900

tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc      960

aaaatgtcgt aacaactgcg atcgcccgcc ccgttgacgc aaatgggcgg taggcgtgta     1020

cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcac tagaagcttt     1080

attgcggtag tttatcacag ttaaattgct aacgcagtca gtgcttctga cacaacagtc     1140

tcgaacttaa gctgcagtga ctctcttaag gtagccttgc agaagttggt cgtgaggcac     1200

tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga aactgggctt     1260

gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta ctgacatcca     1320

ctttgccttt ctctccacag gtgtccactc ccagttcaat tacagctctt aaggctagag     1380

tacttaatac gactcactat aggctagcct cgagctcaag cttcgaattc gaatggccat     1440

ggagtttggg ctgagctggc tttttcttgt ggctatttta aaaggtgtcc agtgctctag     1500

agacatccag atgacacaga ctacatcctc cctgtctgcc tctctgggag acagagtcac     1560

catcagttgc agggcaagtc aggacattag taaatattta aattggtatc agcaaaaacc     1620

agatggaact gttaaactcc tgatctacca tacatcaaga ttacactcag gagtcccatc     1680

aaggttcagt ggcagtgggt ctggaacaga ttattctctc accattagca acctggagca     1740

agaagatatt gccacttact tttgccaaca gggtaatacg cttccgtaca cgttcggagg     1800

ggggaccaag ctggagctga aacgtggtgg tggtggttct ggtggtggtg gttctggtaa     1860

gcctatccct aaccctctcc tcggtctcga ttctacgggc ggcggcggct ccggtggtgg     1920

tggatccgag gtgcagctgc agcagtctgg acctggcctg gtggcgccct cacagagcct     1980

gtccgtcaca tgcactgtct caggggtctc attacccgac tatggtgtaa gctggattcg     2040

ccagcctcca cgaaagggtc tggagtggct gggagtaata tggggtagtg aaaccacata     2100

ctataattca gctctcaaat ccagactgac catcatcaag gacaactcca agagccaagt     2160

tttcttaaaa atgaacagtc tgcaaactga tgacacagcc atttactact gtgccaaaca     2220

ttattactac ggtggtagct atgctatgga ctactggggc caagggacca cggtcaccgt     2280

ctcctcgtac gtcaccgtct cttcacagga tcccgccgag cccaaatctc ctgacaaaac     2340

tcacacatgc ccaccgtgcc cagcacctga actcctgggg ggaccgtcag tcttcctctt     2400

ccccccaaaa cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt     2460

ggtggacgtg agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga     2520

ggtgcataat gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt     2580

cagcgtcctc accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt     2640

ctccaacaaa gccctcccag cccccatcga gaaaaccatc tccaaagcca aagggcagcc     2700

ccgagaacca caggtgtaca ccctgccccc atcccgggat gagctgacca agaaccaggt     2760

cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag     2820

caatgggcaa ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc     2880

cttcttcctc tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt     2940

ctcatgctcc gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct     3000

gtctccgggt aaaaaagatc ccaaattttg ggtgctggtg gtggttggtg gagtcctggc     3060

ttgctatagc ttgctagtaa cagtggcctt tattattttc tgggtgagga gtaagaggag     3120

caggctcctg cacagtgact acatgaacat gactccccgc cgccccgggc ccacccgcaa     3180

gcattaccag ccctatgccc caccacgcga cttcgcagcc tatcgctcca gagtgaagtt     3240

cagcaggagc gcagacgccc ccgcgtacca gcagggccag aaccagctct ataacgagct     3300

caatctagga cgaagagagg agtacgatgt tttggacaag agacgtggcc gggaccctga     3360

gatgggggga aagccgagaa ggaagaaccc tcaggaaggc ctgtacaatg aactgcagaa     3420

agataagatg gcggaggcct acagtgagat tgggatgaaa ggcgagcgcc ggaggggcaa     3480

ggggcacgat ggcctttacc agggtctcag tacagccacc aaggacacct acgacgccct     3540

tcacatgcag gccctgcctc ctcgctaagc atgctagcta tagttctaga ggtaccggtt     3600

gttaacgtta gccggctacg tatactccgg aatattaata ggcctaggat gcatatggcg     3660

gccgcttccc tttagtgagg gttaatgctt cgagcagaca tgataagata cattgatgag     3720

tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat     3780

gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc     3840

attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac     3900

ctctacaaat gtggtaaaat ccgataagga tcgatccggg ctggcgtaat agcgaagagg     3960

cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg     4020

tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc     4080

cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgcccg     4140

atagcgataa ggatccgcgt atggtgcact ctcagtacaa tctgctctga tgccgcatag     4200

ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc     4260

ccggcatccg cttacagaca agctgtgacc gtctccggga ttttgttact ttatagaaga     4320

aattttgagt ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa     4380

tttattatta gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa     4440

taaacctcga tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta     4500

acgtacgtca caatatgatt atctttctag ggttaa                               4536

<210>  2
<211>  1785
<212>  DNA
<213>  human

<400>  2
aatgggtagt tctttagacg atgagcatat cctctctgct cttctgcaaa gcgatgacga       60

gcttgttggt gaggattctg acagtgaaat atcagatcac gtaagtgaag atgacgtcca      120

gagcgataca gaagaagcgt ttatagatga ggtacatgaa gtgcagccaa cgtcaagcgg      180

tagtgaaata ttagacgaac aaaatgttat tgaacaacca ggttcttcat tggcttctaa      240

cagaatcttg accttgccac agaggactat tagaggtaag aataaacatt gttggtcaac      300

ttcaaagtcc acgaggcgta gccgagtctc tgcactgaac attgtcagat ctcaaagagg      360

tccgacgcgt atgtgccgca atatatatga cccactttta tgcttcaaac tattttttac      420

tgatgagata atttcggaaa ttgtaaaatg gacaaatgct gagatatcat tgaaacgtcg      480

ggaatctatg acaggtgcta catttcgtga cacgaatgaa gatgaaatct atgctttctt      540

tggtattctg gtaatgacag cagtgagaaa agataaccac atgtccacag atgacctctt      600

tgatcgatct ttgtcaatgg tgtacgtctc tgtaatgagt cgtgatcgtt ttgatttttt      660

gatacgatgt cttagaatgg atgacaaaag tatacggccc acacttcgag aaaacgatgt      720

atttactcct gttagaaaaa tatgggatct ctttatccat cagtgcatac aaaattacac      780

tccaggggct catttgacca tagatgaaca gttacttggt tttagaggac ggtgtccgtt      840

taggatgtat atcccaaaca agccaagtaa gtatggaata aaaatcctca tgatgtgtga      900

cagtggtacg aagtatatga taaatggaat gccttatttg ggaagaggaa cacagaccaa      960

cggagtacca ctcggtgaat actacgtgaa ggagttatca aagcctgtgc acggtagttg     1020

tcgtaatatt acgtgtgaca attggttcac ctcaatccct ttggcaaaaa acttactaca     1080

agaaccgtat aagttaacca ttgtgggaac cgtgcgatca aacaaacgcg agataccgga     1140

agtactgaaa aacagtcgct ccaggccagt gggaacatcg atgttttgtt ttgacggacc     1200

ccttactctc gtctcatata aaccgaagcc agctaagatg gtatacttat tatcatcttg     1260

tgatgaggat gcttctatca acgaaagtac cggtaaaccg caaatggtta tgtattataa     1320

tcaaactaaa ggcggagtgg acacgctaga ccaaatgtgt tctgtgatga cctgcagtag     1380

gaagacgaat aggtggccta tggcattatt gtacggaatg ataaacattg cctgcataaa     1440

ttcttttatt atatacagcc ataatgtcag tagcaaggga gaaaaggttc aaagtcgcaa     1500

aaaatttatg agaaaccttt acatgagcct gacgtcatcg tttatgcgta agcgtttaga     1560

agctcctact ttgaagagat atttgcgcga taatatctct aatattttgc caaatgaagt     1620

gcctggtaca tcagatgaca gtactgaaga gccagtaatg aaaaaacgta cttactgtac     1680

ttactgcccc tctaaaataa ggcgaaaggc aaatgcatcg tgcaaaaaat gcaaaaaagt     1740

tatttgtcga gagcataata ttgatatgtg ccaaagttgt ttctg                     1885

<220>
<223>  Nucleic acid condensing peptide

<400>  3

<210>  3
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nucleic acid condensing peptide

<400>  3

Arg Gln Arg Gln Arg Tyr Tyr Arg Gln Arg Gln Arg Gly Gly Arg Arg 
1               5                   10                  15      


Arg Arg Arg Arg 
            20  


<210>  4
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nucleic acid condensing peptide

<400>  4

Arg Gln Arg Gln Arg Gly Gly Arg Arg Arg Arg Arg Arg 
1               5                   10              


<210>  5
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nucleic acid condensing peptide

<400>  5

Arg Arg Arg Arg Arg Arg Tyr Tyr Arg Gln Arg Gln Arg Gly Gly Arg 
1               5                   10                  15      


Arg Arg Arg Arg Arg 
            20      




<210>  6
<211>  39
<212>  PRT
<213>  human

<400>  6

Gly Asn Gln Ser Ser Asn Phe Gly Pro Met Lys Gly Gly Asn Phe Gly 
1               5                   10                  15      


Gly Arg Ser Ser Gly Pro Tyr Gly Gly Gly Gly Gln Tyr Phe Ala Lys 
            20                  25                  30          


Pro Arg Asn Gln Gly Gly Tyr 
        35                  
