                         SEQUENCE LISTING

<110>  Ospedale Pediatrico Bambino Ges
 
<120>  CAR-CD123 vector and uses thereof

<130>  PCT 143735

<150>  EP 19188360.2
<151>  2019-07-25

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  333
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met 
1               5                   10                  15      


Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp 
            20                  25                  30          


Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln 
        35                  40                  45              


Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu 
    50                  55                  60                  


Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile 
65                  70                  75                  80  


Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu 
                85                  90                  95      


Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr 
            100                 105                 110         


Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp 
        115                 120                 125             


Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro 
    130                 135                 140                 


Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala 
145                 150                 155                 160 


Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Leu Pro Pro 
                165                 170                 175     


Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro 
            180                 185                 190         


Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser 
        195                 200                 205             


Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser 
    210                 215                 220                 


Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp 
225                 230                 235                 240 


Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala 
                245                 250                 255     


Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu 
            260                 265                 270         


Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly 
        275                 280                 285             


Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu 
    290                 295                 300                 


Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg 
305                 310                 315                 320 


Arg Lys Arg Lys Arg Met Thr Asp Pro Thr Arg Arg Phe 
                325                 330             


<210>  2
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2A peptide

<400>  2

Arg Gly Arg Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  3
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal

<400>  3

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Ser Arg 
            20      


<210>  4
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7G3 VL

<400>  4

Asp Phe Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr Leu Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg 
        


<210>  5
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7G3 VH

<400>  5

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Tyr Met Lys Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Asp Ile Ile Pro Ser Asn Gly Ala Thr Phe Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met His Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Ser His Leu Leu Arg Ala Ser Trp Phe Ala Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val 
        115             


<210>  6
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  trackable marker

<400>  6

Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser 
1               5                   10                  15      


<210>  7
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  7

Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
1               5                   10                  15      


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
            20                  25                  30          


His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40          


<210>  8
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  8

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  9
<211>  16
<212>  PRT
<213>  Homo sapiens

<400>  9

Leu Tyr Cys Asn His Arg Asn Arg Arg Arg Val Cys Lys Cys Pro Arg 
1               5                   10                  15      


<210>  10
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  10

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  11
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  11

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  12
<211>  869
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  deltaCD19-2A-CAR.CD123-4.1BB-zeta CAR

<400>  12

Met Pro Pro Pro Arg Leu Leu Phe Phe Leu Leu Phe Leu Thr Pro Met 
1               5                   10                  15      


Glu Val Arg Pro Glu Glu Pro Leu Val Val Lys Val Glu Glu Gly Asp 
            20                  25                  30          


Asn Ala Val Leu Gln Cys Leu Lys Gly Thr Ser Asp Gly Pro Thr Gln 
        35                  40                  45              


Gln Leu Thr Trp Ser Arg Glu Ser Pro Leu Lys Pro Phe Leu Lys Leu 
    50                  55                  60                  


Ser Leu Gly Leu Pro Gly Leu Gly Ile His Met Arg Pro Leu Ala Ile 
65                  70                  75                  80  


Trp Leu Phe Ile Phe Asn Val Ser Gln Gln Met Gly Gly Phe Tyr Leu 
                85                  90                  95      


Cys Gln Pro Gly Pro Pro Ser Glu Lys Ala Trp Gln Pro Gly Trp Thr 
            100                 105                 110         


Val Asn Val Glu Gly Ser Gly Glu Leu Phe Arg Trp Asn Val Ser Asp 
        115                 120                 125             


Leu Gly Gly Leu Gly Cys Gly Leu Lys Asn Arg Ser Ser Glu Gly Pro 
    130                 135                 140                 


Ser Ser Pro Ser Gly Lys Leu Met Ser Pro Lys Leu Tyr Val Trp Ala 
145                 150                 155                 160 


Lys Asp Arg Pro Glu Ile Trp Glu Gly Glu Pro Pro Cys Leu Pro Pro 
                165                 170                 175     


Arg Asp Ser Leu Asn Gln Ser Leu Ser Gln Asp Leu Thr Met Ala Pro 
            180                 185                 190         


Gly Ser Thr Leu Trp Leu Ser Cys Gly Val Pro Pro Asp Ser Val Ser 
        195                 200                 205             


Arg Gly Pro Leu Ser Trp Thr His Val His Pro Lys Gly Pro Lys Ser 
    210                 215                 220                 


Leu Leu Ser Leu Glu Leu Lys Asp Asp Arg Pro Ala Arg Asp Met Trp 
225                 230                 235                 240 


Val Met Glu Thr Gly Leu Leu Leu Pro Arg Ala Thr Ala Gln Asp Ala 
                245                 250                 255     


Gly Lys Tyr Tyr Cys His Arg Gly Asn Leu Thr Met Ser Phe His Leu 
            260                 265                 270         


Glu Ile Thr Ala Arg Pro Val Leu Trp His Trp Leu Leu Arg Thr Gly 
        275                 280                 285             


Gly Trp Lys Val Ser Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu 
    290                 295                 300                 


Cys Ser Leu Val Gly Ile Leu His Leu Gln Arg Ala Leu Val Leu Arg 
305                 310                 315                 320 


Arg Lys Arg Lys Arg Met Thr Asp Pro Thr Arg Arg Phe Arg Gly Arg 
                325                 330                 335     


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
            340                 345                 350         


Pro Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys 
        355                 360                 365             


Gly Val Gln Cys Ser Arg Asp Phe Val Met Thr Gln Ser Pro Ser Ser 
    370                 375                 380                 


Leu Thr Val Thr Ala Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser 
385                 390                 395                 400 


Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr 
                405                 410                 415     


Leu Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser 
            420                 425                 430         


Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly 
        435                 440                 445             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala 
    450                 455                 460                 


Val Tyr Tyr Cys Gln Asn Asp Tyr Ser Tyr Pro Tyr Thr Phe Gly Gly 
465                 470                 475                 480 


Gly Thr Lys Leu Glu Ile Lys Arg Gly Gly Gly Ser Gly Gly Gly Gly 
                485                 490                 495     


Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
            500                 505                 510         


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
        515                 520                 525             


Tyr Met Lys Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
    530                 535                 540                 


Gly Asp Ile Ile Pro Ser Asn Gly Ala Thr Phe Tyr Asn Gln Lys Phe 
545                 550                 555                 560 


Lys Gly Lys Ala Thr Leu Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr 
                565                 570                 575     


Met His Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
            580                 585                 590         


Thr Arg Ser His Leu Leu Arg Ala Ser Trp Phe Ala Tyr Trp Gly Gln 
        595                 600                 605             


Gly Thr Leu Val Thr Val Ser Ala Gly Ser Glu Leu Pro Thr Gln Gly 
    610                 615                 620                 


Thr Phe Ser Asn Val Ser Thr Asn Val Ser Pro Ala Pro Arg Pro Pro 
625                 630                 635                 640 


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
                645                 650                 655     


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
            660                 665                 670         


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
        675                 680                 685             


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
    690                 695                 700                 


Arg Arg Arg Val Cys Lys Cys Pro Arg Val Asp Lys Arg Gly Arg Lys 
705                 710                 715                 720 


Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr 
                725                 730                 735     


Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu 
            740                 745                 750         


Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
        755                 760                 765             


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
    770                 775                 780                 


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
785                 790                 795                 800 


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
                805                 810                 815     


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
            820                 825                 830         


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
        835                 840                 845             


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
    850                 855                 860                 


Ala Leu Pro Pro Arg 
865                 


<210>  13
<211>  999
<212>  DNA
<213>  Homo sapiens

<400>  13
atgccaccac ctcgcctgct gttctttctg ctgttcctga cacctatgga ggtgcgacct       60

gaggaaccac tggtcgtgaa ggtcgaggaa ggcgacaatg ccgtgctgca gtgcctgaaa      120

ggcacttctg atgggccaac tcagcagctg acctggtcca gggagtctcc cctgaagcct      180

tttctgaaac tgagcctggg actgccagga ctgggaatcc acatgcgccc tctggctatc      240

tggctgttca tcttcaacgt gagccagcag atgggaggat tctacctgtg ccagccagga      300

ccaccatccg agaaggcctg gcagcctgga tggaccgtca acgtggaggg gtctggagaa      360

ctgtttaggt ggaatgtgag tgacctggga ggactgggat gtgggctgaa gaaccgctcc      420

tctgaaggcc caagttcacc ctcagggaag ctgatgagcc caaaactgta cgtgtgggcc      480

aaagatcggc ccgagatctg ggagggagaa cctccatgcc tgccacctag agacagcctg      540

aatcagagtc tgtcacagga tctgacaatg gcccccgggt ccactctgtg gctgtcttgt      600

ggagtcccac ccgacagcgt gtccagaggc cctctgtcct ggacccacgt gcatcctaag      660

gggccaaaaa gtctgctgtc actggaactg aaggacgatc ggcctgccag agacatgtgg      720

gtcatggaga ctggactgct gctgccacga gcaaccgcac aggatgctgg aaaatactat      780

tgccaccggg gcaatctgac aatgtccttc catctggaga tcactgcaag gcccgtgctg      840

tggcactggc tgctgcgaac cggaggatgg aaggtcagtg ctgtgacact ggcatatctg      900

atcttttgcc tgtgctccct ggtgggcatt ctgcatctgc agagagccct ggtgctgcgg      960

agaaagagaa agagaatgac tgacccaaca agaaggttt                             999


<210>  14
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2A peptide

<400>  14
cgcggccgcg gccgagggag cctgctgaca tgtggcgatg tggaggaaaa cccaggacca       60


<210>  15
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  15
atggagtttg gactttcttg gttgtttttg gtggcaattc tgaagggtgt ccagtgtagc       60

agg                                                                     63


<210>  16
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  7G3 VL

<400>  16
gactttgtaa tgacccaatc tccaagctct cttacggtaa cggcaggaga gaaagtcacc       60

atgtcatgta aatccagtca atccctcttg aactcaggca accagaaaaa ttatcttacg      120

tggtatcttc aaaagccggg gcaaccccca aaactcctga tctactgggc atcaaccagg      180

gagtccggcg tccccgaccg ctttacgggt agtggaagtg gaaccgattt tacccttact      240

atcagcagcg tacaagcgga agacttggct gtgtattatt gtcaaaatga ttattcatat      300

ccctatactt tcggtggagg gactaaactt gaaattaaac ga                         342


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  flex

<400>  17
ggcggaggaa gcggaggtgg gggc                                              24


<210>  18
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VH 7G3

<400>  18
gaagtccagt tgcaacaatc tggccctgag ttggtaaagc ccggagcctc tgtgaagatg       60

agttgtaagg cttcagggta tacatttaca gactattata tgaaatgggt caaacaatct      120

cacggtaaat ccttggagtg gattggcgat attatcccga gtaacggtgc cacgttctac      180

aaccagaagt tcaagggcaa ggcaacactg acggtagacc gcagcagcag cacggcgtat      240

atgcacctga actcattgac ttcagaggat agtgcagttt actactgtac tcggagtcat      300

ttgctgagag cgagttggtt cgcctattgg ggtcagggca cactcgttac cgta            354


<210>  19
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  link

<400>  19
tctgcaggat cc                                                           12


<210>  20
<211>  48
<212>  DNA
<213>  Homo sapiens

<400>  20
gaacttccta ctcaggggac tttctcaaac gttagcacaa acgtaagt                    48


<210>  21
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spacer

<400>  21
cccgccccaa gaccccccac a                                                 21


<210>  22
<211>  126
<212>  DNA
<213>  Homo sapiens

<400>  22
cccgccccaa gaccccccac acctgcgccg accattgctt ctcaacccct gagtttgaga       60

cccgaggcct gccggccagc tgccggcggg gccgtgcata caagaggact cgatttcgct      120

tgcgac                                                                 126


<210>  23
<211>  63
<212>  DNA
<213>  Homo sapiens

<400>  23
atctatatct gggcacctct cgctggcacc tgtggagtcc ttctgctcag cctggttatt       60

act                                                                     63


<210>  24
<211>  48
<212>  DNA
<213>  Homo sapiens

<400>  24
ctgtactgta atcaccggaa tcgccgccgc gtttgtaagt gtcccagg                    48


<210>  25
<211>  126
<212>  DNA
<213>  Homo sapiens

<400>  25
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactg                                                                 126


<210>  26
<211>  339
<212>  DNA
<213>  Homo sapiens

<400>  26
agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgctag                             339


<210>  27
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  27

Gly Gly Gly Ser Gly Gly Gly Gly 
1               5               


<210>  28
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  28

Ser Ala Gly Ser 
1               


<210>  29
<211>  2610
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding CD123 chimeric antigen receptor

<400>  29
atgccaccac ctcgcctgct gttctttctg ctgttcctga cacctatgga ggtgcgacct       60

gaggaaccac tggtcgtgaa ggtcgaggaa ggcgacaatg ccgtgctgca gtgcctgaaa      120

ggcacttctg atgggccaac tcagcagctg acctggtcca gggagtctcc cctgaagcct      180

tttctgaaac tgagcctggg actgccagga ctgggaatcc acatgcgccc tctggctatc      240

tggctgttca tcttcaacgt gagccagcag atgggaggat tctacctgtg ccagccagga      300

ccaccatccg agaaggcctg gcagcctgga tggaccgtca acgtggaggg gtctggagaa      360

ctgtttaggt ggaatgtgag tgacctggga ggactgggat gtgggctgaa gaaccgctcc      420

tctgaaggcc caagttcacc ctcagggaag ctgatgagcc caaaactgta cgtgtgggcc      480

aaagatcggc ccgagatctg ggagggagaa cctccatgcc tgccacctag agacagcctg      540

aatcagagtc tgtcacagga tctgacaatg gcccccgggt ccactctgtg gctgtcttgt      600

ggagtcccac ccgacagcgt gtccagaggc cctctgtcct ggacccacgt gcatcctaag      660

gggccaaaaa gtctgctgtc actggaactg aaggacgatc ggcctgccag agacatgtgg      720

gtcatggaga ctggactgct gctgccacga gcaaccgcac aggatgctgg aaaatactat      780

tgccaccggg gcaatctgac aatgtccttc catctggaga tcactgcaag gcccgtgctg      840

tggcactggc tgctgcgaac cggaggatgg aaggtcagtg ctgtgacact ggcatatctg      900

atcttttgcc tgtgctccct ggtgggcatt ctgcatctgc agagagccct ggtgctgcgg      960

agaaagagaa agagaatgac tgacccaaca agaaggtttc gcggccgcgg ccgagggagc     1020

ctgctgacat gtggcgatgt ggaggaaaac ccaggaccaa tggagtttgg actttcttgg     1080

ttgtttttgg tggcaattct gaagggtgtc cagtgtagca gggactttgt aatgacccaa     1140

tctccaagct ctcttacggt aacggcagga gagaaagtca ccatgtcatg taaatccagt     1200

caatccctct tgaactcagg caaccagaaa aattatctta cgtggtatct tcaaaagccg     1260

gggcaacccc caaaactcct gatctactgg gcatcaacca gggagtccgg cgtccccgac     1320

cgctttacgg gtagtggaag tggaaccgat tttaccctta ctatcagcag cgtacaagcg     1380

gaagacttgg ctgtgtatta ttgtcaaaat gattattcat atccctatac tttcggtgga     1440

gggactaaac ttgaaattaa acgaggcgga ggaagcggag gtgggggcga agtccagttg     1500

caacaatctg gccctgagtt ggtaaagccc ggagcctctg tgaagatgag ttgtaaggct     1560

tcagggtata catttacaga ctattatatg aaatgggtca aacaatctca cggtaaatcc     1620

ttggagtgga ttggcgatat tatcccgagt aacggtgcca cgttctacaa ccagaagttc     1680

aagggcaagg caacactgac ggtagaccgc agcagcagca cggcgtatat gcacctgaac     1740

tcattgactt cagaggatag tgcagtttac tactgtactc ggagtcattt gctgagagcg     1800

agttggttcg cctattgggg tcagggcaca ctcgttaccg tatctgcagg atccgaactt     1860

cctactcagg ggactttctc aaacgttagc acaaacgtaa gtcccgcccc aagacccccc     1920

acacctgcgc cgaccattgc ttctcaaccc ctgagtttga gacccgaggc ctgccggcca     1980

gctgccggcg gggccgtgca tacaagagga ctcgatttcg cttgcgacat ctatatctgg     2040

gcacctctcg ctggcacctg tggagtcctt ctgctcagcc tggttattac tctgtactgt     2100

aatcaccgga atcgccgccg cgtttgtaag tgtcccaggg tcgacaaacg gggcagaaag     2160

aaactcctgt atatattcaa acaaccattt atgagaccag tacaaactac tcaagaggaa     2220

gatggctgta gctgccgatt tccagaagaa gaagaaggag gatgtgaact gagagtgaag     2280

ttcagcagga gcgcagacgc ccccgcgtac cagcagggcc agaaccagct ctataacgag     2340

ctcaatctag gacgaagaga ggagtacgat gttttggaca agagacgtgg ccgggaccct     2400

gagatggggg gaaagccgag aaggaagaac cctcaggaag gcctgtacaa tgaactgcag     2460

aaagataaga tggcggaggc ctacagtgag attgggatga aaggcgagcg ccggaggggc     2520

aaggggcacg atggccttta ccagggtctc agtacagcca ccaaggacac ctacgacgcc     2580

cttcacatgc aggccctgcc ccctcgctag                                      2610


<210>  30
<211>  240
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  30

Asp Phe Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr Leu Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys Arg Gly Gly Gly Ser Gly Gly Gly Gly Glu Val Gln Leu Gln Gln 
        115                 120                 125             


Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys Met Ser Cys 
    130                 135                 140                 


Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr Tyr Met Lys Trp Val Lys 
145                 150                 155                 160 


Gln Ser His Gly Lys Ser Leu Glu Trp Ile Gly Asp Ile Ile Pro Ser 
                165                 170                 175     


Asn Gly Ala Thr Phe Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu 
            180                 185                 190         


Thr Val Asp Arg Ser Ser Ser Thr Ala Tyr Met His Leu Asn Ser Leu 
        195                 200                 205             


Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Thr Arg Ser His Leu Leu 
    210                 215                 220                 


Arg Ala Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val 
225                 230                 235                 240 


<210>  31
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  aa. 12-42 of SEQ ID NO: 7

<400>  31

Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
1               5                   10                  15      


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
            20                  25                  30      


<210>  32
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  aa. 1-11 of SEQ ID NO:7

<400>  32

Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
1               5                   10      


<210>  33
<211>  229
<212>  PRT
<213>  Mus musculus

<400>  33

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  34
<211>  222
<212>  PRT
<213>  Homo sapiens

<400>  34

Lys Glu Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys 
1               5                   10                  15      


Lys Ala Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn 
            20                  25                  30          


Gln Thr Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val 
        35                  40                  45              


Val Ser Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu 
    50                  55                  60                  


Gln Ser Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg 
65                  70                  75                  80  


Cys Ala Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala 
                85                  90                  95      


Cys Arg Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp 
            100                 105                 110         


Lys Gln Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp 
        115                 120                 125             


Glu Ala Asn His Val Asp Pro Cys Leu Pro Cys Thr Val Cys Glu Asp 
    130                 135                 140                 


Thr Glu Arg Gln Leu Arg Glu Cys Thr Arg Trp Ala Asp Ala Glu Cys 
145                 150                 155                 160 


Glu Glu Ile Pro Gly Arg Trp Ile Thr Arg Ser Thr Pro Pro Glu Gly 
                165                 170                 175     


Ser Asp Ser Thr Ala Pro Ser Thr Gln Glu Pro Glu Ala Pro Pro Glu 
            180                 185                 190         


Gln Asp Leu Ile Ala Ser Thr Val Ala Gly Val Val Thr Thr Val Met 
        195                 200                 205             


Gly Ser Ser Gln Pro Val Val Thr Arg Gly Thr Thr Asp Asn 
    210                 215                 220         


<210>  35
<211>  39
<212>  PRT
<213>  Homo sapiens

<400>  35

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro 
        35                  


<210>  36
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge CH3

<400>  36

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Gly Gln Pro Arg 
1               5                   10                  15      


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
            20                  25                  30          


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        35                  40                  45              


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    50                  55                  60                  


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
65                  70                  75                  80  


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
                85                  90                  95      


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            100                 105                 110         


Leu Ser Leu Ser Leu Gly Lys 
        115                 


<210>  37
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  37

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  38
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  38

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  39
<211>  36
<212>  PRT
<213>  Homo sapiens

<400>  39

Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly 
1               5                   10                  15      


Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr 
            20                  25                  30          


Leu Ala Lys Ile 
        35      


<210>  40
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  core sequence motif


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  40

Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               


<210>  41
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide

<400>  41

Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  42
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide

<400>  42

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  43
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide

<400>  43

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  44
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide

<400>  44

Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val 
1               5                   10                  15      


Glu Ser Asn Pro Gly Pro 
            20          


<210>  45
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  45

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  46
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  46

Pro Lys Pro Ser Thr Pro Pro Gly Ser Ser 
1               5                   10  


<210>  47
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  47

Pro Lys Pro Ser Thr Pro Pro Gly Ser Ser Pro Lys Pro Ser Thr Pro 
1               5                   10                  15      


Pro Gly Ser Ser 
            20  


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  48

Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   


<210>  49
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  49

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  50
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  50

Gly Gly Gly Gly Ser Gly Gly 
1               5           


<210>  51
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  51

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys 
            


