                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals
 
<120>  QUANTIFICATION OF BIOCONJUGATE GLYCOSYLATION

<130>  VB66747 WO

<150>  19187963.4
<151>  2019-07-23

<160>  53

<170>  PatentIn version 3.5

<210>  1
<211>  613
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  1

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu 
                245                 250                 255     


Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe 
            260                 265                 270         


Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly 
        275                 280                 285             


Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser 
    290                 295                 300                 


Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly 
305                 310                 315                 320 


Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala 
                325                 330                 335     


Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg 
            340                 345                 350         


Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val 
        355                 360                 365             


Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp 
    370                 375                 380                 


Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe 
385                 390                 395                 400 


Leu Gly Asp Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn 
                405                 410                 415     


Trp Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg 
            420                 425                 430         


Gly Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln 
        435                 440                 445             


Ser Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala 
    450                 455                 460                 


Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly 
465                 470                 475                 480 


Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly 
                485                 490                 495     


Ala Leu Leu Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr 
            500                 505                 510         


Arg Thr Gly Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu 
        515                 520                 525             


Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly 
    530                 535                 540                 


Pro Glu Glu Glu Gly Gly Arg Leu Glu Thr Ile Leu Gly Trp Pro Leu 
545                 550                 555                 560 


Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg 
                565                 570                 575     


Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln 
            580                 585                 590         


Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro 
        595                 600                 605             


Arg Glu Asp Leu Lys 
    610             


<210>  2
<211>  612
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of EPA with L552V/deltaE553 detoxifying 
       mutation

<400>  2

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu 
                245                 250                 255     


Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe 
            260                 265                 270         


Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly 
        275                 280                 285             


Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser 
    290                 295                 300                 


Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly 
305                 310                 315                 320 


Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala 
                325                 330                 335     


Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg 
            340                 345                 350         


Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val 
        355                 360                 365             


Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp 
    370                 375                 380                 


Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe 
385                 390                 395                 400 


Leu Gly Asp Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn 
                405                 410                 415     


Trp Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg 
            420                 425                 430         


Gly Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln 
        435                 440                 445             


Ser Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala 
    450                 455                 460                 


Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly 
465                 470                 475                 480 


Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly 
                485                 490                 495     


Ala Leu Leu Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr 
            500                 505                 510         


Arg Thr Gly Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu 
        515                 520                 525             


Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly 
    530                 535                 540                 


Pro Glu Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala 
545                 550                 555                 560 


Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn 
                565                 570                 575     


Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala 
            580                 585                 590         


Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg 
        595                 600                 605             


Glu Asp Leu Lys 
    610         


<210>  3
<211>  1315
<212>  PRT
<213>  Clostridium tetani

<400>  3

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile 
        435                 440                 445             


Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly 
    450                 455                 460                 


Glu Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu 
465                 470                 475                 480 


Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr 
                485                 490                 495     


Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile 
            500                 505                 510         


Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg 
        515                 520                 525             


Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser 
    530                 535                 540                 


Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile 
545                 550                 555                 560 


Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile 
                565                 570                 575     


Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile 
            580                 585                 590         


Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln 
        595                 600                 605             


Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr 
    610                 615                 620                 


Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser 
625                 630                 635                 640 


Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly 
                645                 650                 655     


Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu 
            660                 665                 670         


Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu 
        675                 680                 685             


Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile 
    690                 695                 700                 


Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys 
705                 710                 715                 720 


Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys 
                725                 730                 735     


Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile 
            740                 745                 750         


Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys 
        755                 760                 765             


Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu 
    770                 775                 780                 


Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser 
785                 790                 795                 800 


Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln 
                805                 810                 815     


Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile 
            820                 825                 830         


Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu 
        835                 840                 845             


Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser 
    850                 855                 860                 


Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile 
865                 870                 875                 880 


Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile 
                885                 890                 895     


Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala 
            900                 905                 910         


Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn 
        915                 920                 925             


Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn 
    930                 935                 940                 


Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys 
945                 950                 955                 960 


Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 
                965                 970                 975     


Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser 
            980                 985                 990         


Val Ser Leu Lys Gly Asn Asn Leu  Ile Trp Thr Leu Lys  Asp Ser Ala 
        995                 1000                 1005             


Gly Glu  Val Arg Gln Ile Thr  Phe Arg Asp Leu Pro  Asp Lys Phe 
    1010                 1015                 1020             


Asn Ala  Tyr Leu Ala Asn Lys  Trp Val Phe Ile Thr  Ile Thr Asn 
    1025                 1030                 1035             


Asp Arg  Leu Ser Ser Ala Asn  Leu Tyr Ile Asn Gly  Val Leu Met 
    1040                 1045                 1050             


Gly Ser  Ala Glu Ile Thr Gly  Leu Gly Ala Ile Arg  Glu Asp Asn 
    1055                 1060                 1065             


Asn Ile  Thr Leu Lys Leu Asp  Arg Cys Asn Asn Asn  Asn Gln Tyr 
    1070                 1075                 1080             


Val Ser  Ile Asp Lys Phe Arg  Ile Phe Cys Lys Ala  Leu Asn Pro 
    1085                 1090                 1095             


Lys Glu  Ile Glu Lys Leu Tyr  Thr Ser Tyr Leu Ser  Ile Thr Phe 
    1100                 1105                 1110             


Leu Arg  Asp Phe Trp Gly Asn  Pro Leu Arg Tyr Asp  Thr Glu Tyr 
    1115                 1120                 1125             


Tyr Leu  Ile Pro Val Ala Ser  Ser Ser Lys Asp Val  Gln Leu Lys 
    1130                 1135                 1140             


Asn Ile  Thr Asp Tyr Met Tyr  Leu Thr Asn Ala Pro  Ser Tyr Thr 
    1145                 1150                 1155             


Asn Gly  Lys Leu Asn Ile Tyr  Tyr Arg Arg Leu Tyr  Asn Gly Leu 
    1160                 1165                 1170             


Lys Phe  Ile Ile Lys Arg Tyr  Thr Pro Asn Asn Glu  Ile Asp Ser 
    1175                 1180                 1185             


Phe Val  Lys Ser Gly Asp Phe  Ile Lys Leu Tyr Val  Ser Tyr Asn 
    1190                 1195                 1200             


Asn Asn  Glu His Ile Val Gly  Tyr Pro Lys Asp Gly  Asn Ala Phe 
    1205                 1210                 1215             


Asn Asn  Leu Asp Arg Ile Leu  Arg Val Gly Tyr Asn  Ala Pro Gly 
    1220                 1225                 1230             


Ile Pro  Leu Tyr Lys Lys Met  Glu Ala Val Lys Leu  Arg Asp Leu 
    1235                 1240                 1245             


Lys Thr  Tyr Ser Val Gln Leu  Lys Leu Tyr Asp Asp  Lys Asn Ala 
    1250                 1255                 1260             


Ser Leu  Gly Leu Val Gly Thr  His Asn Gly Gln Ile  Gly Asn Asp 
    1265                 1270                 1275             


Pro Asn  Arg Asp Ile Leu Ile  Ala Ser Asn Trp Tyr  Phe Asn His 
    1280                 1285                 1290             


Leu Lys  Asp Lys Ile Leu Gly  Cys Asp Trp Tyr Phe  Val Pro Thr 
    1295                 1300                 1305             


Asp Glu  Gly Trp Thr Asn Asp  
    1310                 1315 


<210>  4
<211>  535
<212>  PRT
<213>  Corynebacterium diphtheriae

<400>  4

Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn 
1               5                   10                  15      


Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln 
            20                  25                  30          


Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp 
        35                  40                  45              


Asp Trp Lys Gly Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly 
    50                  55                  60                  


Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val 
65                  70                  75                  80  


Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val 
                85                  90                  95      


Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu 
            100                 105                 110         


Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly 
        115                 120                 125             


Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser 
    130                 135                 140                 


Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser 
145                 150                 155                 160 


Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp 
                165                 170                 175     


Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg 
            180                 185                 190         


Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val 
        195                 200                 205             


Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly 
    210                 215                 220                 


Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu 
225                 230                 235                 240 


Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu 
                245                 250                 255     


His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val 
            260                 265                 270         


Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val 
        275                 280                 285             


Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu 
    290                 295                 300                 


Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 
305                 310                 315                 320 


Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser 
                325                 330                 335     


Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp 
            340                 345                 350         


Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe 
        355                 360                 365             


Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His 
    370                 375                 380                 


Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr 
385                 390                 395                 400 


Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His 
                405                 410                 415     


Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val 
            420                 425                 430         


Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr 
        435                 440                 445             


His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile 
    450                 455                 460                 


Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly 
465                 470                 475                 480 


Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 
                485                 490                 495     


Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu 
            500                 505                 510         


Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser 
        515                 520                 525             


Leu Phe Phe Glu Ile Lys Ser 
    530                 535 


<210>  5
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CRM197, non-toxic mutant of diphtheria toxin

<400>  5

Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn 
1               5                   10                  15      


Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln 
            20                  25                  30          


Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp 
        35                  40                  45              


Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly 
    50                  55                  60                  


Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val 
65                  70                  75                  80  


Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val 
                85                  90                  95      


Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu 
            100                 105                 110         


Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly 
        115                 120                 125             


Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser 
    130                 135                 140                 


Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser 
145                 150                 155                 160 


Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp 
                165                 170                 175     


Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg 
            180                 185                 190         


Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val 
        195                 200                 205             


Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly 
    210                 215                 220                 


Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu 
225                 230                 235                 240 


Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu 
                245                 250                 255     


His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val 
            260                 265                 270         


Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val 
        275                 280                 285             


Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu 
    290                 295                 300                 


Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 
305                 310                 315                 320 


Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser 
                325                 330                 335     


Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp 
            340                 345                 350         


Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe 
        355                 360                 365             


Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His 
    370                 375                 380                 


Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr 
385                 390                 395                 400 


Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His 
                405                 410                 415     


Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val 
            420                 425                 430         


Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr 
        435                 440                 445             


His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile 
    450                 455                 460                 


Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly 
465                 470                 475                 480 


Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 
                485                 490                 495     


Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu 
            500                 505                 510         


Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser 
        515                 520                 525             


Leu Phe Phe Glu Ile Lys Ser 
    530                 535 


<210>  6
<211>  162
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  6

Met Ala Val Asp Met Phe Ile Lys Ile Gly Asp Val Lys Gly Glu Ser 
1               5                   10                  15      


Lys Asp Lys Thr His Ala Glu Glu Ile Asp Val Leu Ala Trp Ser Trp 
            20                  25                  30          


Gly Met Ser Gln Ser Gly Ser Met His Met Gly Gly Gly Gly Gly Ala 
        35                  40                  45              


Gly Lys Val Asn Val Gln Asp Leu Ser Phe Thr Lys Tyr Ile Asp Lys 
    50                  55                  60                  


Ser Thr Pro Asn Leu Met Met Ala Cys Ser Ser Gly Lys His Tyr Pro 
65                  70                  75                  80  


Gln Ala Lys Leu Thr Ile Arg Lys Ala Gly Gly Glu Asn Gln Val Glu 
                85                  90                  95      


Tyr Leu Ile Ile Thr Leu Lys Glu Val Leu Val Ser Ser Val Ser Thr 
            100                 105                 110         


Gly Gly Ser Gly Gly Glu Asp Arg Leu Thr Glu Asn Val Thr Leu Asn 
        115                 120                 125             


Phe Ala Gln Val Gln Val Asp Tyr Gln Pro Gln Lys Ala Asp Gly Ala 
    130                 135                 140                 


Lys Asp Gly Gly Pro Ile Lys Tyr Gly Trp Asn Ile Arg Gln Asn Val 
145                 150                 155                 160 


Gln Ala 
        


<210>  7
<211>  221
<212>  PRT
<213>  Escherichia coli

<400>  7

Gly Ile Phe Ser Arg Phe Ala Asp Ile Val Asn Ala Asn Ile Asn Ala 
1               5                   10                  15      


Leu Leu Glu Lys Ala Glu Asp Pro Gln Lys Leu Val Arg Leu Met Ile 
            20                  25                  30          


Gln Glu Met Glu Asp Thr Leu Val Glu Val Arg Ser Thr Ser Ala Arg 
        35                  40                  45              


Ala Leu Ala Glu Lys Lys Gln Leu Thr Arg Arg Ile Glu Gln Ala Ser 
    50                  55                  60                  


Ala Arg Glu Val Glu Trp Gln Glu Lys Ala Glu Leu Ala Leu Leu Lys 
65                  70                  75                  80  


Glu Arg Glu Asp Leu Ala Arg Ala Ala Leu Ile Glu Lys Gln Lys Leu 
                85                  90                  95      


Thr Asp Leu Ile Lys Ser Leu Glu His Glu Val Thr Leu Val Asp Asp 
            100                 105                 110         


Thr Leu Ala Arg Met Lys Lys Glu Ile Gly Glu Leu Glu Asn Lys Leu 
        115                 120                 125             


Ser Glu Thr Arg Ala Arg Gln Gln Ala Leu Met Leu Arg His Gln Ala 
    130                 135                 140                 


Ala Asn Ser Ser Arg Asp Val Arg Arg Gln Leu Asp Ser Gly Lys Leu 
145                 150                 155                 160 


Asp Glu Ala Met Ala Arg Phe Glu Ser Phe Glu Arg Arg Ile Asp Gln 
                165                 170                 175     


Met Glu Ala Glu Ala Glu Ser His Ser Phe Gly Lys Gln Lys Ser Leu 
            180                 185                 190         


Asp Asp Gln Phe Ala Glu Leu Lys Ala Asp Asp Ala Ile Ser Glu Gln 
        195                 200                 205             


Leu Ala Gln Leu Lys Ala Lys Met Lys Gln Asp Asn Gln 
    210                 215                 220     


<210>  8
<211>  396
<212>  PRT
<213>  Escherichia coli

<400>  8

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ile Glu Glu Gly Lys 
            20                  25                  30          


Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu 
        35                  40                  45              


Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu 
    50                  55                  60                  


His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly 
65                  70                  75                  80  


Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr 
                85                  90                  95      


Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln 
            100                 105                 110         


Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys 
        115                 120                 125             


Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn 
    130                 135                 140                 


Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala 
145                 150                 155                 160 


Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn 
                165                 170                 175     


Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly 
            180                 185                 190         


Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly 
        195                 200                 205             


Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu 
    210                 215                 220                 


Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu 
225                 230                 235                 240 


Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp 
                245                 250                 255     


Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val 
            260                 265                 270         


Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu 
        275                 280                 285             


Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu 
    290                 295                 300                 


Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn 
305                 310                 315                 320 


Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu 
                325                 330                 335     


Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys 
            340                 345                 350         


Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala 
        355                 360                 365             


Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp 
    370                 375                 380                 


Glu Ala Leu Lys Asp Ala Gln Thr Arg Ile Thr Lys 
385                 390                 395     


<210>  9
<211>  445
<212>  PRT
<213>  Neisseria gonorrhoeae

<400>  9

Met Ile Pro Gln Tyr Glu Gln Pro Lys Val Glu Val Ala Glu Thr Phe 
1               5                   10                  15      


Gln Asn Asp Thr Ser Val Ser Ser Ile Arg Ala Val Asp Leu Gly Trp 
            20                  25                  30          


His Asp Tyr Phe Ala Asp Pro Arg Leu Gln Lys Leu Ile Asp Ile Ala 
        35                  40                  45              


Leu Glu Arg Asn Thr Ser Leu Arg Thr Ala Val Leu Asn Ser Glu Ile 
    50                  55                  60                  


Tyr Arg Lys Gln Tyr Met Ile Glu Arg Asn Asn Leu Leu Pro Thr Leu 
65                  70                  75                  80  


Ala Ala Asn Ala Asn Gly Ser Arg Gln Gly Ser Leu Ser Gly Gly Asn 
                85                  90                  95      


Val Ser Ser Ser Tyr Asn Val Gly Leu Gly Ala Ala Ser Tyr Glu Leu 
            100                 105                 110         


Asp Leu Phe Gly Arg Val Arg Ser Ser Ser Glu Ala Ala Leu Gln Gly 
        115                 120                 125             


Tyr Phe Ala Ser Val Ala Asn Arg Asp Ala Ala His Leu Ser Leu Ile 
    130                 135                 140                 


Ala Thr Val Ala Lys Ala Tyr Phe Asn Glu Arg Tyr Ala Glu Glu Ala 
145                 150                 155                 160 


Met Ser Leu Ala Gln Arg Val Leu Lys Thr Arg Glu Glu Thr Tyr Asn 
                165                 170                 175     


Ala Val Arg Ile Ala Val Gln Gly Arg Arg Asp Phe Arg Arg Arg Pro 
            180                 185                 190         


Ala Pro Ala Glu Ala Leu Ile Glu Ser Ala Lys Ala Asp Tyr Ala His 
        195                 200                 205             


Ala Ala Arg Ser Arg Glu Gln Ala Arg Asn Ala Leu Ala Thr Leu Ile 
    210                 215                 220                 


Asn Arg Pro Ile Pro Glu Asp Leu Pro Ala Gly Leu Pro Leu Asp Lys 
225                 230                 235                 240 


Gln Phe Phe Val Glu Lys Leu Pro Ala Gly Leu Ser Ser Glu Val Leu 
                245                 250                 255     


Leu Asp Arg Pro Asp Ile Arg Ala Ala Glu His Ala Leu Lys Gln Ala 
            260                 265                 270         


Asn Ala Asn Ile Gly Ala Ala Arg Ala Ala Phe Phe Pro Ser Ile Arg 
        275                 280                 285             


Leu Thr Gly Ser Val Gly Thr Gly Ser Val Glu Leu Gly Gly Leu Phe 
    290                 295                 300                 


Lys Ser Gly Thr Gly Val Trp Ala Phe Ala Pro Ser Ile Thr Leu Pro 
305                 310                 315                 320 


Ile Phe Thr Trp Gly Thr Asn Lys Ala Asn Leu Asp Val Ala Lys Leu 
                325                 330                 335     


Arg Gln Gln Ala Gln Ile Val Ala Tyr Glu Ser Ala Val Gln Ser Ala 
            340                 345                 350         


Phe Gln Asp Val Ala Asn Ala Leu Ala Ala Arg Glu Gln Leu Asp Lys 
        355                 360                 365             


Ala Tyr Asp Ala Leu Ser Lys Gln Ser Arg Ala Ser Lys Glu Ala Leu 
    370                 375                 380                 


Arg Leu Val Gly Leu Arg Tyr Lys His Gly Val Ser Gly Ala Leu Asp 
385                 390                 395                 400 


Leu Leu Asp Ala Glu Arg Ser Ser Tyr Ser Ala Glu Gly Ala Ala Leu 
                405                 410                 415     


Ser Ala Gln Leu Thr Arg Ala Glu Asn Leu Ala Asp Leu Tyr Lys Ala 
            420                 425                 430         


Leu Gly Gly Gly Leu Lys Arg Asp Thr Gln Thr Gly Lys 
        435                 440                 445 


<210>  10
<211>  522
<212>  PRT
<213>  Staphylococcus aureus

<400>  10

Ala Ser Glu Asn Ser Val Thr Gln Ser Asp Ser Ala Ser Asn Glu Ser 
1               5                   10                  15      


Lys Ser Asn Asp Ser Ser Ser Val Ser Ala Ala Pro Lys Thr Asp Asp 
            20                  25                  30          


Thr Asn Val Ser Asp Thr Lys Thr Ser Ser Asn Thr Asn Asn Gly Glu 
        35                  40                  45              


Thr Ser Val Ala Gln Asn Pro Ala Gln Gln Glu Thr Thr Gln Ser Ser 
    50                  55                  60                  


Ser Thr Asn Ala Thr Thr Glu Glu Thr Pro Val Thr Gly Glu Ala Thr 
65                  70                  75                  80  


Thr Thr Thr Thr Asn Gln Ala Asn Thr Pro Ala Thr Thr Gln Ser Ser 
                85                  90                  95      


Asn Thr Asn Ala Glu Glu Leu Val Asn Gln Thr Ser Asn Glu Thr Thr 
            100                 105                 110         


Phe Asn Asp Thr Asn Thr Val Ser Ser Val Asn Ser Pro Gln Asn Ser 
        115                 120                 125             


Thr Asn Ala Glu Asn Val Ser Thr Thr Gln Asp Thr Ser Thr Glu Ala 
    130                 135                 140                 


Thr Pro Ser Asn Asn Glu Ser Ala Pro Gln Ser Thr Asp Ala Ser Asn 
145                 150                 155                 160 


Lys Asp Val Val Asn Gln Ala Val Asn Thr Ser Ala Pro Arg Met Arg 
                165                 170                 175     


Ala Phe Ser Leu Ala Ala Val Ala Ala Asp Ala Pro Ala Ala Gly Thr 
            180                 185                 190         


Asp Ile Thr Asn Gln Leu Thr Asn Val Thr Val Gly Ile Asp Ser Gly 
        195                 200                 205             


Thr Thr Val Tyr Pro His Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly 
    210                 215                 220                 


Phe Ser Val Pro Asn Ser Ala Val Lys Gly Asp Thr Phe Lys Ile Thr 
225                 230                 235                 240 


Val Pro Lys Glu Leu Asn Leu Asn Gly Val Thr Ser Thr Ala Lys Val 
                245                 250                 255     


Pro Pro Ile Met Ala Gly Asp Gln Val Leu Ala Asn Gly Val Ile Asp 
            260                 265                 270         


Ser Asp Gly Asn Val Ile Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys 
        275                 280                 285             


Asp Asp Val Lys Ala Thr Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu 
    290                 295                 300                 


Asn Val Lys Lys Thr Gly Asn Val Thr Leu Ala Thr Gly Ile Gly Ser 
305                 310                 315                 320 


Thr Thr Ala Asn Lys Thr Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys 
                325                 330                 335     


Phe Tyr Asn Leu Ser Ile Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr 
            340                 345                 350         


Asn Asn Thr Tyr Arg Gln Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn 
        355                 360                 365             


Val Ile Ala Pro Val Leu Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser 
    370                 375                 380                 


Asn Ala Leu Ile Asp Gln Gln Asn Thr Ser Ile Lys Val Tyr Lys Val 
385                 390                 395                 400 


Asp Asn Ala Ala Asp Leu Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn 
                405                 410                 415     


Phe Glu Asp Val Thr Asn Ser Val Asn Ile Thr Phe Pro Asn Pro Asn 
            420                 425                 430         


Gln Tyr Lys Val Glu Phe Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro 
        435                 440                 445             


Tyr Ile Val Val Val Asn Gly His Ile Asp Pro Asn Ser Lys Gly Asp 
    450                 455                 460                 


Leu Ala Leu Arg Ser Thr Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp 
465                 470                 475                 480 


Arg Ser Met Ser Trp Asp Asn Glu Val Ala Phe Asn Asn Gly Ser Gly 
                485                 490                 495     


Ser Gly Asp Gly Ile Asp Lys Pro Val Val Pro Glu Gln Pro Asp Glu 
            500                 505                 510         


Pro Gly Glu Ile Glu Pro Ile Pro Glu Asp 
        515                 520         


<210>  11
<211>  340
<212>  PRT
<213>  Staphylococcus aureus

<400>  11

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Pro Ala Tyr Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Ile Pro Glu Asp 
            340 


<210>  12
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ClfAN2N3P116S/Y118A

<400>  12

Val Ala Ala Asp Ala Pro Ala Ala Gly Thr Asp Ile Thr Asn Gln Leu 
1               5                   10                  15      


Thr Asn Val Thr Val Gly Ile Asp Ser Gly Thr Thr Val Tyr Pro His 
            20                  25                  30          


Gln Ala Gly Tyr Val Lys Leu Asn Tyr Gly Phe Ser Val Pro Asn Ser 
        35                  40                  45              


Ala Val Lys Gly Asp Thr Phe Lys Ile Thr Val Pro Lys Glu Leu Asn 
    50                  55                  60                  


Leu Asn Gly Val Thr Ser Thr Ala Lys Val Pro Pro Ile Met Ala Gly 
65                  70                  75                  80  


Asp Gln Val Leu Ala Asn Gly Val Ile Asp Ser Asp Gly Asn Val Ile 
                85                  90                  95      


Tyr Thr Phe Thr Asp Tyr Val Asn Thr Lys Asp Asp Val Lys Ala Thr 
            100                 105                 110         


Leu Thr Met Ser Ala Ala Ile Asp Pro Glu Asn Val Lys Lys Thr Gly 
        115                 120                 125             


Asn Val Thr Leu Ala Thr Gly Ile Gly Ser Thr Thr Ala Asn Lys Thr 
    130                 135                 140                 


Val Leu Val Asp Tyr Glu Lys Tyr Gly Lys Phe Tyr Asn Leu Ser Ile 
145                 150                 155                 160 


Lys Gly Thr Ile Asp Gln Ile Asp Lys Thr Asn Asn Thr Tyr Arg Gln 
                165                 170                 175     


Thr Ile Tyr Val Asn Pro Ser Gly Asp Asn Val Ile Ala Pro Val Leu 
            180                 185                 190         


Thr Gly Asn Leu Lys Pro Asn Thr Asp Ser Asn Ala Leu Ile Asp Gln 
        195                 200                 205             


Gln Asn Thr Ser Ile Lys Val Tyr Lys Val Asp Asn Ala Ala Asp Leu 
    210                 215                 220                 


Ser Glu Ser Tyr Phe Val Asn Pro Glu Asn Phe Glu Asp Val Thr Asn 
225                 230                 235                 240 


Ser Val Asn Ile Thr Phe Pro Asn Pro Asn Gln Tyr Lys Val Glu Phe 
                245                 250                 255     


Asn Thr Pro Asp Asp Gln Ile Thr Thr Pro Tyr Ile Val Val Val Asn 
            260                 265                 270         


Gly His Ile Asp Pro Asn Ser Lys Gly Asp Leu Ala Leu Arg Ser Thr 
        275                 280                 285             


Leu Tyr Gly Tyr Asn Ser Asn Ile Ile Trp Arg Ser Met Ser Trp Asp 
    290                 295                 300                 


Asn Glu Val Ala Phe Asn Asn Gly Ser Gly Ser Gly Asp Gly Ile Asp 
305                 310                 315                 320 


Lys Pro Val Val Pro Glu Gln Pro Asp Glu Pro Gly Glu Ile Glu Pro 
                325                 330                 335     


Ile Pro Glu Asp 
            340 


<210>  13
<211>  293
<212>  PRT
<213>  Staphylococcus aureus

<400>  13

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  14
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature HlaH35L

<400>  14

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Gly Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  15
<211>  293
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/H48C/G122C

<400>  15

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His 
    130                 135                 140                 


Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro 
145                 150                 155                 160 


Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn 
                165                 170                 175     


Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly 
            180                 185                 190         


Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp 
        195                 200                 205             


Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe 
    210                 215                 220                 


Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys 
225                 230                 235                 240 


Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr 
                245                 250                 255     


Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp 
            260                 265                 270         


Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys 
        275                 280                 285             


Glu Glu Met Thr Asn 
    290             


<210>  16
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HlaPSGS

<400>  16

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met His Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn His 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Pro Ser Gly 
            100                 105                 110         


Ser Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys 
        115                 120                 125             


Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp 
    130                 135                 140                 


Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu 
145                 150                 155                 160 


Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu 
                165                 170                 175     


Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp 
            180                 185                 190         


Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr 
        195                 200                 205             


Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His 
    210                 215                 220                 


Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile 
225                 230                 235                 240 


Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met 
                245                 250                 255     


Thr Asn 
        


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be Ser or Thr

<400>  17

Xaa Xaa Asn Xaa Xaa 
1               5   


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa can be Ser or Thr

<400>  18

Lys Xaa Xaa Asn Xaa Xaa Lys 
1               5           


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa can be Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa is any amino acid except lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is any amino acid except lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa can be Lys or Arg

<400>  19

Xaa Xaa Xaa Asn Xaa Xaa Xaa Xaa Xaa 
1               5                   


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid apart from proline

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa can be Ser or Thr

<400>  20

Lys Xaa Xaa Asn Xaa Xaa Ser Ala Arg 
1               5                   


<210>  21
<211>  19
<212>  PRT
<213>  Escherichia coli

<400>  21

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala 
            


<210>  22
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  22

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  23
<211>  26
<212>  PRT
<213>  Escherichia coli

<400>  23

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  24
<211>  22
<212>  PRT
<213>  Erwinia carotovora

<400>  24

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  25
<211>  23
<212>  PRT
<213>  Escherichia coli

<400>  25

Met Ser Phe Lys Lys Ile Ile Lys Ala Phe Val Ile Met Ala Ala Leu 
1               5                   10                  15      


Val Ser Val Gln Ala His Ala 
            20              


<210>  26
<211>  28
<212>  PRT
<213>  Escherichia coli

<400>  26

Met Phe Lys Phe Lys Lys Lys Phe Leu Val Gly Leu Thr Ala Ala Phe 
1               5                   10                  15      


Met Ser Ile Ser Met Phe Ser Ala Thr Ala Ser Ala 
            20                  25              


<210>  27
<211>  19
<212>  PRT
<213>  Escherichia coli

<400>  27

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210>  28
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  28

Met Lys Gln Ala Leu Arg Val Ala Phe Gly Phe Leu Ile Leu Trp Ala 
1               5                   10                  15      


Ser Val Leu His Ala 
            20      


<210>  29
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SipA signal sequence

<400>  29

Met Lys Met Asn Lys Lys Val Leu Leu Thr Ser Thr Met Ala Ala Ser 
1               5                   10                  15      


Leu Leu Ser Val Ala Ser Val Gln Ala Ser 
            20                  25      


<210>  30
<211>  325
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of Hla H35L/H48C/G122C with N-terminal S, 
       Flgl signal sequence, C-terminal GSHRHR, and KDQNRTK substitution
       for residue K131

<400>  30

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly 
145                 150                 155                 160 


Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln 
                165                 170                 175     


Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly 
            180                 185                 190         


Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr 
        195                 200                 205             


Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys 
    210                 215                 220                 


Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn 
225                 230                 235                 240 


Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr 
                245                 250                 255     


Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp 
            260                 265                 270         


Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser 
        275                 280                 285             


Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser 
    290                 295                 300                 


Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn Gly 
305                 310                 315                 320 


Ser His Arg His Arg 
                325 


<210>  31
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with N-terminal
       S, Flgl signal sequence, C-terminal GSHRHR and KDSNITSAR 
       substitution for residue K131

<400>  31

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Asp Ser Asn Ile Thr Ser Ala Arg Ile 
145                 150                 155                 160 


Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr 
                165                 170                 175     


Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys 
            180                 185                 190         


Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly 
        195                 200                 205             


Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe 
    210                 215                 220                 


Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp 
225                 230                 235                 240 


Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe 
                245                 250                 255     


Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn 
            260                 265                 270         


Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp 
        275                 280                 285             


Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp 
    290                 295                 300                 


Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr 
305                 310                 315                 320 


Asn Gly Ser His Arg His Arg 
                325         


<210>  32
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with N-terminal
       S, Flgl signal sequence, C-terminal GSHRHR and KDSNSTSAR 
       substitution for residue K131

<400>  32

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Asp Ser Asn Ser Thr Ser Ala Arg Ile 
145                 150                 155                 160 


Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr 
                165                 170                 175     


Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys 
            180                 185                 190         


Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly 
        195                 200                 205             


Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe 
    210                 215                 220                 


Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp 
225                 230                 235                 240 


Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe 
                245                 250                 255     


Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn 
            260                 265                 270         


Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp 
        275                 280                 285             


Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp 
    290                 295                 300                 


Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr 
305                 310                 315                 320 


Asn Gly Ser His Arg His Arg 
                325         


<210>  33
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with N-terminal
       S, Flgl signal sequence, C-terminal GSHRHR and KDSNVTSAR 
       substitution for residue K131,

<400>  33

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Asp Ser Asn Val Thr Ser Ala Arg Ile 
145                 150                 155                 160 


Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr 
                165                 170                 175     


Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys 
            180                 185                 190         


Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly 
        195                 200                 205             


Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe 
    210                 215                 220                 


Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp 
225                 230                 235                 240 


Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe 
                245                 250                 255     


Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn 
            260                 265                 270         


Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp 
        275                 280                 285             


Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp 
    290                 295                 300                 


Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr 
305                 310                 315                 320 


Asn Gly Ser His Arg His Arg 
                325         


<210>  34
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with N-terminal
       S, Flgl signal sequence, C-terminal GSHRHR and KDSNATSAR 
       substitution for residue K131

<400>  34

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr 
            20                  25                  30          


Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr 
        35                  40                  45              


Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp 
    50                  55                  60                  


Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr 
65                  70                  75                  80  


Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser 
                85                  90                  95      


Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp 
            100                 105                 110         


Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp 
        115                 120                 125             


Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val 
    130                 135                 140                 


Thr Gly Asp Asp Thr Gly Lys Asp Ser Asn Val Thr Ser Ala Arg Ile 
145                 150                 155                 160 


Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr 
                165                 170                 175     


Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys 
            180                 185                 190         


Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly 
        195                 200                 205             


Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe 
    210                 215                 220                 


Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp 
225                 230                 235                 240 


Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe 
                245                 250                 255     


Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn 
            260                 265                 270         


Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp 
        275                 280                 285             


Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp 
    290                 295                 300                 


Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr 
305                 310                 315                 320 


Asn Gly Ser His Arg His Arg 
                325         


<210>  35
<211>  299
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/H48C/G122C with KDQNRTK 
       substitution for residue K131

<400>  35

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Gln Asn Arg Thr Lys Ile Gly Gly Leu Ile Gly Ala 
    130                 135                 140                 


Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys 
145                 150                 155                 160 


Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile 
                165                 170                 175     


Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser 
            180                 185                 190         


Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly 
        195                 200                 205             


Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser 
    210                 215                 220                 


Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met 
225                 230                 235                 240 


Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu 
                245                 250                 255     


Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys 
            260                 265                 270         


Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr 
        275                 280                 285             


Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 


<210>  36
<211>  301
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with KDSNITSAR 
       substitution for residue K131

<400>  36

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Ser Asn Ile Thr Ser Ala Arg Ile Gly Gly Leu Ile 
    130                 135                 140                 


Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp 
145                 150                 155                 160 


Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys 
                165                 170                 175     


Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg 
            180                 185                 190         


Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg 
        195                 200                 205             


Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala 
    210                 215                 220                 


Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile 
225                 230                 235                 240 


Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile 
                245                 250                 255     


Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn 
            260                 265                 270         


Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu 
        275                 280                 285             


Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 300     


<210>  37
<211>  301
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with KDSNSTSAR 
       substitution for residue K131

<400>  37

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Ser Asn Ser Thr Ser Ala Arg Ile Gly Gly Leu Ile 
    130                 135                 140                 


Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp 
145                 150                 155                 160 


Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys 
                165                 170                 175     


Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg 
            180                 185                 190         


Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg 
        195                 200                 205             


Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala 
    210                 215                 220                 


Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile 
225                 230                 235                 240 


Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile 
                245                 250                 255     


Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn 
            260                 265                 270         


Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu 
        275                 280                 285             


Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 300     


<210>  38
<211>  301
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with KDSNVTSAR 
       substitution for residue K131

<400>  38

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Ser Asn Val Thr Ser Ala Arg Ile Gly Gly Leu Ile 
    130                 135                 140                 


Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp 
145                 150                 155                 160 


Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys 
                165                 170                 175     


Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg 
            180                 185                 190         


Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg 
        195                 200                 205             


Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala 
    210                 215                 220                 


Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile 
225                 230                 235                 240 


Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile 
                245                 250                 255     


Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn 
            260                 265                 270         


Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu 
        275                 280                 285             


Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 300     


<210>  39
<211>  301
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with KDSNATSAR 
       substitution for residue K131

<400>  39

Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser 
1               5                   10                  15      


Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn 
            20                  25                  30          


Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys 
        35                  40                  45              


Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln 
    50                  55                  60                  


Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp 
65                  70                  75                  80  


Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro Asp Asn Glu Val Ala 
                85                  90                  95      


Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr 
            100                 105                 110         


Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn Val Thr Gly Asp Asp 
        115                 120                 125             


Thr Gly Lys Asp Ser Asn Val Thr Ser Ala Arg Ile Gly Gly Leu Ile 
    130                 135                 140                 


Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro Asp 
145                 150                 155                 160 


Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp Lys 
                165                 170                 175     


Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg 
            180                 185                 190         


Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg 
        195                 200                 205             


Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala 
    210                 215                 220                 


Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val Ile 
225                 230                 235                 240 


Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val Ile 
                245                 250                 255     


Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr Asn 
            260                 265                 270         


Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu 
        275                 280                 285             


Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn 
    290                 295                 300     


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDQNRTK glycosite

<400>  40

Lys Asp Gln Asn Arg Thr Lys 
1               5           


<210>  41
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDQNATK glycosite

<400>  41

Lys Asp Gln Asn Ala Thr Lys 
1               5           


<210>  42
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDSNITSAR glycosite

<400>  42

Lys Asp Ser Asn Ile Thr Ser Ala Arg 
1               5                   


<210>  43
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDSNSTSAR glycosite

<400>  43

Lys Asp Ser Asn Ser Thr Ser Ala Arg 
1               5                   


<210>  44
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDSNVTSAR glycosite

<400>  44

Lys Asp Ser Asn Val Thr Ser Ala Arg 
1               5                   


<210>  45
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  KDSNATSAR glycosite

<400>  45

Lys Asp Ser Asn Ala Thr Ser Ala Arg 
1               5                   


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Zaa represent 0-9 amino acids which are any amino acid except 
       cysteine, methionine, asparagine, glutamine, lysine or arginine.

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is any amino acid except proline, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is any amino acid except proline, lysine or arginine

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa can be Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Zaa represent 0-9 amino acids which are any amino acid except 
       cysteine, methionine, asparagine, glutamine, lysine or arginine.

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa can be Lys or Arg

<400>  46

Xaa Xaa Xaa Xaa Asn Xaa Xaa Xaa Xaa 
1               5                   


<210>  47
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be Lys or Arg

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa represent 0-9 amino acids which are any amino acid except 
       cysteine, methionine, asparagine, glutamine, lysine or arginine.

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is any amino acid except proline, cysteine, methionine, 
       asparagine, glutamine, lysine or arginine.

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa is any amino acid except proline, cysteine, methionine, 
       asparagine, glutamine, lysine or arginine.

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa can be Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa represent 0-9 amino acids which are any amino acid except 
       cysteine, methionine, asparagine, glutamine, lysine or arginine.

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa can be Lys or Arg

<400>  47

Xaa Xaa Xaa Xaa Asn Xaa Xaa Xaa Xaa 
1               5                   


<210>  48
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Peptide 42T-50K named PTP-2

<400>  48

Thr Gly Asp Leu Val Thr Tyr Lys 
1               5               


<210>  49
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Peptide 225A-234K named PTP-3

<400>  49

Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys 
1               5                   10  


<210>  50
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Spacer

<400>  50

Gly Ser Gly Gly Gly 
1               5   


<210>  51
<211>  351
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with KDSNITSAR 
       glycosite substitution for residue K131; glycosite KDSNVTSAR at 
       N-terminal with GSGGG spacers before and after this glycosite; 
       Flgl signal sequence; and His tag at C-terminal

<400>  51

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Gly Ser Gly Gly Gly Lys Asp Ser Asn Val Thr 
            20                  25                  30          


Ser Ala Arg Gly Ser Gly Gly Gly Lys Leu Ala Asp Ser Asp Ile Asn 
        35                  40                  45              


Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser Asn Thr Thr Val Lys Thr 
    50                  55                  60                  


Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn Gly Met Leu Lys Lys Val 
65                  70                  75                  80  


Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys Asn Lys Lys Leu Leu Val 
                85                  90                  95      


Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu 
            100                 105                 110         


Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp Pro Ser Ala Phe Lys Val 
        115                 120                 125             


Gln Leu Gln Leu Pro Asp Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr 
    130                 135                 140                 


Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr 
145                 150                 155                 160 


Gly Phe Asn Cys Asn Val Thr Gly Asp Asp Thr Gly Lys Asp Ser Asn 
                165                 170                 175     


Ile Thr Ser Ala Arg Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile 
            180                 185                 190         


Gly His Thr Leu Lys Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu 
        195                 200                 205             


Ser Pro Thr Asp Lys Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met 
    210                 215                 220                 


Val Asn Gln Asn Trp Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val 
225                 230                 235                 240 


Tyr Gly Asn Gln Leu Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala 
                245                 250                 255     


Ala Asp Asn Phe Leu Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser 
            260                 265                 270         


Gly Phe Ser Pro Asp Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala 
        275                 280                 285             


Ser Lys Gln Gln Thr Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp 
    290                 295                 300                 


Asp Tyr Gln Leu His Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr 
305                 310                 315                 320 


Lys Asp Lys Trp Ile Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp 
                325                 330                 335     


Glu Lys Glu Glu Met Thr Asn Gly Ser His His His His His His 
            340                 345                 350     


<210>  52
<211>  345
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with KDSNITSAR 
       glycosite substitution for residue K131; glycosite KDSNVTSAR at 
       C-terminal with GSGGG spacers before this glycosite; Flgl signal 
       sequence; and His tag at C-terminal

<400>  52

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Ala Asp Ser Asp Ile Asn Ile Lys Thr Gly Thr 
            20                  25                  30          


Thr Asp Ile Gly Ser Asn Thr Thr Val Lys Thr Gly Asp Leu Val Thr 
        35                  40                  45              


Tyr Asp Lys Glu Asn Gly Met Leu Lys Lys Val Phe Tyr Ser Phe Ile 
    50                  55                  60                  


Asp Asp Lys Asn Cys Asn Lys Lys Leu Leu Val Ile Arg Thr Lys Gly 
65                  70                  75                  80  


Thr Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu Glu Gly Ala Asn Lys 
                85                  90                  95      


Ser Gly Leu Ala Trp Pro Ser Ala Phe Lys Val Gln Leu Gln Leu Pro 
            100                 105                 110         


Asp Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr Pro Arg Asn Ser Ile 
        115                 120                 125             


Asp Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr Gly Phe Asn Cys Asn 
    130                 135                 140                 


Val Thr Gly Asp Asp Thr Gly Lys Asp Ser Asn Ile Thr Ser Ala Arg 
145                 150                 155                 160 


Ile Gly Gly Leu Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys 
                165                 170                 175     


Tyr Val Gln Pro Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys 
            180                 185                 190         


Lys Val Gly Trp Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp 
        195                 200                 205             


Gly Pro Tyr Asp Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu 
    210                 215                 220                 


Phe Met Lys Thr Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu 
225                 230                 235                 240 


Asp Pro Asn Lys Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp 
                245                 250                 255     


Phe Ala Thr Val Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr 
            260                 265                 270         


Asn Ile Asp Val Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His 
        275                 280                 285             


Trp Thr Ser Thr Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile 
    290                 295                 300                 


Asp Arg Ser Ser Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met 
305                 310                 315                 320 


Thr Asn Leu Gly Ser Gly Gly Gly Lys Asp Ser Asn Val Thr Ser Ala 
                325                 330                 335     


Arg Gly Ser His His His His His His 
            340                 345 


<210>  53
<211>  357
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Amino acid sequence of mature Hla H35L/G122C/H48C with KDSNITSAR 
       glycosite at C-terminal end preceded by GSGGG spacers; glycosite 
       KDSNVTSAR at N-terminal with GSGGG spacers before and after this 
       glycosite; Flgl signal sequence; and His tag at C-terminal

<400>  53

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala Ser Ala Gly Ser Gly Gly Gly Lys Asp Ser Asn Val Thr 
            20                  25                  30          


Ser Ala Arg Gly Ser Gly Gly Gly Lys Leu Ala Asp Ser Asp Ile Asn 
        35                  40                  45              


Ile Lys Thr Gly Thr Thr Asp Ile Gly Ser Asn Thr Thr Val Lys Thr 
    50                  55                  60                  


Gly Asp Leu Val Thr Tyr Asp Lys Glu Asn Gly Met Leu Lys Lys Val 
65                  70                  75                  80  


Phe Tyr Ser Phe Ile Asp Asp Lys Asn Cys Asn Lys Lys Leu Leu Val 
                85                  90                  95      


Ile Arg Thr Lys Gly Thr Ile Ala Gly Gln Tyr Arg Val Tyr Ser Glu 
            100                 105                 110         


Glu Gly Ala Asn Lys Ser Gly Leu Ala Trp Pro Ser Ala Phe Lys Val 
        115                 120                 125             


Gln Leu Gln Leu Pro Asp Asn Glu Val Ala Gln Ile Ser Asp Tyr Tyr 
    130                 135                 140                 


Pro Arg Asn Ser Ile Asp Thr Lys Glu Tyr Met Ser Thr Leu Thr Tyr 
145                 150                 155                 160 


Gly Phe Asn Cys Asn Val Thr Gly Asp Asp Thr Gly Ile Gly Gly Leu 
                165                 170                 175     


Ile Gly Ala Asn Val Ser Ile Gly His Thr Leu Lys Tyr Val Gln Pro 
            180                 185                 190         


Asp Phe Lys Thr Ile Leu Glu Ser Pro Thr Asp Lys Lys Val Gly Trp 
        195                 200                 205             


Lys Val Ile Phe Asn Asn Met Val Asn Gln Asn Trp Gly Pro Tyr Asp 
    210                 215                 220                 


Arg Asp Ser Trp Asn Pro Val Tyr Gly Asn Gln Leu Phe Met Lys Thr 
225                 230                 235                 240 


Arg Asn Gly Ser Met Lys Ala Ala Asp Asn Phe Leu Asp Pro Asn Lys 
                245                 250                 255     


Ala Ser Ser Leu Leu Ser Ser Gly Phe Ser Pro Asp Phe Ala Thr Val 
            260                 265                 270         


Ile Thr Met Asp Arg Lys Ala Ser Lys Gln Gln Thr Asn Ile Asp Val 
        275                 280                 285             


Ile Tyr Glu Arg Val Arg Asp Asp Tyr Gln Leu His Trp Thr Ser Thr 
    290                 295                 300                 


Asn Trp Lys Gly Thr Asn Thr Lys Asp Lys Trp Ile Asp Arg Ser Ser 
305                 310                 315                 320 


Glu Arg Tyr Lys Ile Asp Trp Glu Lys Glu Glu Met Thr Asn Leu Gly 
                325                 330                 335     


Ser Gly Gly Gly Lys Asp Ser Asn Ile Thr Ser Ala Arg Gly Ser His 
            340                 345                 350         


His His His His His 
        355         
