﻿               序列表

<110> 神州细胞工程有限公司

<120> 嵌合的人乳头瘤病毒16型L1蛋白

<130> CIE200050PCT

<160> 13

<170> BiSSAP 1.3.6

<210> 1
<211> 474
<212> PRT
<213> 智人


<220> 
<223> HPV 16型L1蛋白的1-474氨基酸序列

<400> 1
Met Ser Leu Trp Leu Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 
1               5                   10                  15      
Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ala Arg Thr Asn 
            20                  25                  30          
Ile Tyr Tyr His Ala Gly Thr Ser Arg Leu Leu Ala Val Gly His Pro 
        35                  40                  45              
Tyr Phe Pro Ile Lys Lys Pro Asn Asn Asn Lys Ile Leu Val Pro Lys 
    50                  55                  60                  
Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Ile His Leu Pro Asp Pro 
65                  70                  75                  80  
Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Asp Thr Gln 
                85                  90                  95      
Arg Leu Val Trp Ala Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro 
            100                 105                 110         
Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp 
        115                 120                 125             
Thr Glu Asn Ala Ser Ala Tyr Ala Ala Asn Ala Gly Val Asp Asn Arg 
    130                 135                 140                 
Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly 
145                 150                 155                 160 
Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Ser Pro Cys Thr 
                165                 170                 175     
Asn Val Ala Val Asn Pro Gly Asp Cys Pro Pro Leu Glu Leu Ile Asn 
            180                 185                 190         
Thr Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met 
        195                 200                 205             
Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Glu Val Pro Leu Asp Ile 
    210                 215                 220                 
Cys Thr Ser Ile Cys Lys Tyr Pro Asp Tyr Ile Lys Met Val Ser Glu 
225                 230                 235                 240 
Pro Tyr Gly Asp Ser Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe 
                245                 250                 255     
Val Arg His Leu Phe Asn Arg Ala Gly Ala Val Gly Glu Asn Val Pro 
            260                 265                 270         
Asp Asp Leu Tyr Ile Lys Gly Ser Gly Ser Thr Ala Asn Leu Ala Ser 
        275                 280                 285             
Ser Asn Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala 
    290                 295                 300                 
Gln Ile Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn 
305                 310                 315                 320 
Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr 
                325                 330                 335     
Thr Arg Ser Thr Asn Met Ser Leu Cys Ala Ala Ile Ser Thr Ser Glu 
            340                 345                 350         
Thr Thr Tyr Lys Asn Thr Asn Phe Lys Glu Tyr Leu Arg His Gly Glu 
        355                 360                 365             
Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr 
    370                 375                 380                 
Ala Asp Val Met Thr Tyr Ile His Ser Met Asn Ser Thr Ile Leu Glu 
385                 390                 395                 400 
Asp Trp Asn Phe Gly Leu Gln Pro Pro Pro Gly Gly Thr Leu Glu Asp 
                405                 410                 415     
Thr Tyr Arg Phe Val Thr Ser Gln Ala Ile Ala Cys Gln Lys His Thr 
            420                 425                 430         
Pro Pro Ala Pro Lys Glu Asp Pro Leu Lys Lys Tyr Thr Phe Trp Glu 
        435                 440                 445             
Val Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu 
    450                 455                 460                 
Gly Arg Lys Phe Leu Leu Gln Ala Gly Leu 
465                 470                 

<210> 2
<211> 26
<212> PRT
<213> 智人


<220> 
<223> HPV 33型L1蛋白的474-499氨基酸序列

<400> 2
Lys Ala Lys Pro Lys Leu Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr 
1               5                   10                  15      
Ser Ser Ala Lys Arg Lys Lys Val Lys Lys 
            20                  25      

<210> 3
<211> 500
<212> PRT
<213> 人工序列


<220> 
<223> 嵌合的HPV 16型L1蛋白的氨基酸序列

<400> 3
Met Ser Leu Trp Leu Pro Ser Glu Ala Thr Val Tyr Leu Pro Pro Val 
1               5                   10                  15      
Pro Val Ser Lys Val Val Ser Thr Asp Glu Tyr Val Ala Arg Thr Asn 
            20                  25                  30          
Ile Tyr Tyr His Ala Gly Thr Ser Arg Leu Leu Ala Val Gly His Pro 
        35                  40                  45              
Tyr Phe Pro Ile Lys Lys Pro Asn Asn Asn Lys Ile Leu Val Pro Lys 
    50                  55                  60                  
Val Ser Gly Leu Gln Tyr Arg Val Phe Arg Ile His Leu Pro Asp Pro 
65                  70                  75                  80  
Asn Lys Phe Gly Phe Pro Asp Thr Ser Phe Tyr Asn Pro Asp Thr Gln 
                85                  90                  95      
Arg Leu Val Trp Ala Cys Val Gly Val Glu Val Gly Arg Gly Gln Pro 
            100                 105                 110         
Leu Gly Val Gly Ile Ser Gly His Pro Leu Leu Asn Lys Leu Asp Asp 
        115                 120                 125             
Thr Glu Asn Ala Ser Ala Tyr Ala Ala Asn Ala Gly Val Asp Asn Arg 
    130                 135                 140                 
Glu Cys Ile Ser Met Asp Tyr Lys Gln Thr Gln Leu Cys Leu Ile Gly 
145                 150                 155                 160 
Cys Lys Pro Pro Ile Gly Glu His Trp Gly Lys Gly Ser Pro Cys Thr 
                165                 170                 175     
Asn Val Ala Val Asn Pro Gly Asp Cys Pro Pro Leu Glu Leu Ile Asn 
            180                 185                 190         
Thr Val Ile Gln Asp Gly Asp Met Val Asp Thr Gly Phe Gly Ala Met 
        195                 200                 205             
Asp Phe Thr Thr Leu Gln Ala Asn Lys Ser Glu Val Pro Leu Asp Ile 
    210                 215                 220                 
Cys Thr Ser Ile Cys Lys Tyr Pro Asp Tyr Ile Lys Met Val Ser Glu 
225                 230                 235                 240 
Pro Tyr Gly Asp Ser Leu Phe Phe Tyr Leu Arg Arg Glu Gln Met Phe 
                245                 250                 255     
Val Arg His Leu Phe Asn Arg Ala Gly Ala Val Gly Glu Asn Val Pro 
            260                 265                 270         
Asp Asp Leu Tyr Ile Lys Gly Ser Gly Ser Thr Ala Asn Leu Ala Ser 
        275                 280                 285             
Ser Asn Tyr Phe Pro Thr Pro Ser Gly Ser Met Val Thr Ser Asp Ala 
    290                 295                 300                 
Gln Ile Phe Asn Lys Pro Tyr Trp Leu Gln Arg Ala Gln Gly His Asn 
305                 310                 315                 320 
Asn Gly Ile Cys Trp Gly Asn Gln Leu Phe Val Thr Val Val Asp Thr 
                325                 330                 335     
Thr Arg Ser Thr Asn Met Ser Leu Cys Ala Ala Ile Ser Thr Ser Glu 
            340                 345                 350         
Thr Thr Tyr Lys Asn Thr Asn Phe Lys Glu Tyr Leu Arg His Gly Glu 
        355                 360                 365             
Glu Tyr Asp Leu Gln Phe Ile Phe Gln Leu Cys Lys Ile Thr Leu Thr 
    370                 375                 380                 
Ala Asp Val Met Thr Tyr Ile His Ser Met Asn Ser Thr Ile Leu Glu 
385                 390                 395                 400 
Asp Trp Asn Phe Gly Leu Gln Pro Pro Pro Gly Gly Thr Leu Glu Asp 
                405                 410                 415     
Thr Tyr Arg Phe Val Thr Ser Gln Ala Ile Ala Cys Gln Lys His Thr 
            420                 425                 430         
Pro Pro Ala Pro Lys Glu Asp Pro Leu Lys Lys Tyr Thr Phe Trp Glu 
        435                 440                 445             
Val Asn Leu Lys Glu Lys Phe Ser Ala Asp Leu Asp Gln Phe Pro Leu 
    450                 455                 460                 
Gly Arg Lys Phe Leu Leu Gln Ala Gly Leu Lys Ala Lys Pro Lys Leu 
465                 470                 475                 480 
Lys Arg Ala Ala Pro Thr Ser Thr Arg Thr Ser Ser Ala Lys Arg Lys 
                485                 490                 495     
Lys Val Lys Lys 
            500 

<210> 4
<211> 1504
<212> DNA
<213> 人工序列


<220> 
<223> 嵌合的HPV 16型L1蛋白的核苷酸序列

<400> 4
atgagtctgt ggctgccatc tgaggctaca gtctacctgc ctcctgtgcc tgtgagcaag      60

gtggtgagca cagatgaata tgtggcaagg accaacatct actaccatgc tggcaccagc     120

agactgctgg ctgtgggaca cccatacttt ccaatcaaga agccaaacaa caacaagatt     180

ctggtgccaa aggtgtctgg actccaatac agggtgttca ggattcacct gcctgaccca     240

aacaagtttg gctttcctga cacctccttc tacaaccctg acacccagag actggtgtgg     300

gcttgtgtgg gagtggaggt gggcagggga caaccactgg gagtgggcat ctctggacac     360

ccactgctga acaaactgga tgacacagag aatgcctctg cctatgctgc caatgctgga     420

gtggacaaca gggagtgtat cagtatggac tacaagcaga cccaactttg tctgattggc     480

tgtaagcctc caattggaga acactggggc aagggcagcc catgtaccaa tgtggctgtg     540

aaccctggag actgtcctcc attggaactg ataaacacag tgattcagga tggagatatg     600

gtggacacag gctttggagc tatggacttc accaccctcc aagccaacaa gtctgaggtg     660

ccactggaca tctgtaccag catctgtaaa taccctgact acatcaagat ggtgtctgaa     720

ccatatggag actccctgtt cttctacctg aggagggaac agatgtttgt gagacacctg     780

ttcaacaggg ctggagcagt gggagagaat gtgcctgatg acctctacat caagggctct     840

ggcagcacag ccaacctggc atccagcaac tactttccaa caccatctgg cagtatggtg     900

acctctgatg cccagatttt caacaagcca tactggctcc aaagggctca aggacacaac     960

aatggcatct gttggggcaa ccaacttttt gtgacagtgg tggacaccac caggagcacc    1020

aatatgagtc tgtgtgctgc catcagcacc tctgagacca cctacaagaa caccaacttc    1080

aaggaatacc tgagacatgg agaggaatat gacctccaat tcatcttcca actttgtaag    1140

attaccctga cagcagatgt gatgacctac atccacagta tgaacagcac catcttggag    1200

gactggaact ttggactcca acctcctcct ggaggcacct tggaggacac ctacaggttt    1260

gtgaccagcc aggctattgc ctgtcagaaa cacacacctc ctgccccaaa ggaggaccca    1320

ctgaaaaaat acaccttctg ggaggtgaac ctgaaagaga agttctctgc tgacctggac    1380

cagtttccac tgggcaggaa gttcctgctc caagcaggac tgaaagccaa gccaaaactg    1440

aaaagggctg ccccaaccag caccaggacc tcctctgcca agaggaagaa ggtgaagaag    1500

taaa                                                                 1504


<210> 5
<211> 1537
<212> DNA
<213> 人工序列


<220> 
<223> 合成的HPV16L1基因

<400> 5
ctgggtacca tgagtctgtg gctgccatct gaggctacag tctacctgcc tcctgtgcct      60

gtgagcaagg tggtgagcac agatgaatat gtggcaagga ccaacatcta ctaccatgct     120

ggcaccagca gactgctggc tgtgggacac ccatactttc caatcaagaa gccaaacaac     180

aacaagattc tggtgccaaa ggtgtctgga ctccaataca gggtgttcag gattcacctg     240

cctgacccaa acaagtttgg ctttcctgac acctccttct acaaccctga cacccagaga     300

ctggtgtggg cttgtgtggg agtggaggtg ggcaggggac aaccactggg agtgggcatc     360

tctggacacc cactgctgaa caaactggat gacacagaga atgcctctgc ctatgctgcc     420

aatgctggag tggacaacag ggagtgtatc agtatggact acaagcagac ccaactttgt     480

ctgattggct gtaagcctcc aattggagaa cactggggca agggcagccc atgtaccaat     540

gtggctgtga accctggaga ctgtcctcca ttggaactga taaacacagt gattcaggat     600

ggagatatgg tggacacagg ctttggagct atggacttca ccaccctcca agccaacaag     660

tctgaggtgc cactggacat ctgtaccagc atctgtaaat accctgacta catcaagatg     720

gtgtctgaac catatggaga ctccctgttc ttctacctga ggagggaaca gatgtttgtg     780

agacacctgt tcaacagggc tggagcagtg ggagagaatg tgcctgatga cctctacatc     840

aagggctctg gcagcacagc caacctggca tccagcaact actttccaac accatctggc     900

agtatggtga cctctgatgc ccagattttc aacaagccat actggctcca aagggctcaa     960

ggacacaaca atggcatctg ttggggcaac caactttttg tgacagtggt ggacaccacc    1020

aggagcacca atatgagtct gtgtgctgcc atcagcacct ctgagaccac ctacaagaac    1080

accaacttca aggaatacct gagacatgga gaggaatatg acctccaatt catcttccaa    1140

ctttgtaaga ttaccctgac agcagatgtg atgacctaca tccacagtat gaacagcacc    1200

atcttggagg actggaactt tggactccaa cctcctcctg gaggcacctt ggaggacacc    1260

tacaggtttg tgaccagcca ggctattgcc tgtcagaaac acacacctcc tgccccaaag    1320

gaggacccac tgaaaaaata caccttctgg gaggtgaacc tgaaagagaa gttctctgct    1380

gacctggacc agtttccact gggcaggaag ttcctgctcc aagcaggact gaaagccaag    1440

ccaaagttca ccctgggcaa gaggaaggct acaccaacca cctccagcac cagcaccaca    1500

gccaagagga agaagaggaa actgtaaact cgagctc                             1537


<210> 6
<211> 1519
<212> DNA
<213> 人工序列


<220> 
<223> 合成的HPV33L1基因

<400> 6
ctgggtacca tgagtgtgtg gagaccatct gaggctacag tctacctgcc tcctgtgcct      60

gtgagcaagg tggtgagcac agatgaatat gtgagcagga ccagcatcta ctactatgct     120

ggctccagca gactgctggc tgtgggacac ccatacttca gcatcaagaa cccaaccaat     180

gccaagaaac tgctggtgcc aaaggtgtct ggactccaat acagggtgtt cagggtgaga     240

ctgcctgacc caaacaagtt tggctttcct gacacctcct tctacaaccc tgacacccag     300

agactggtgt gggcttgtgt gggattggag attggcaggg gacaaccact gggagtgggc     360

atctctggac acccactgct gaacaagttt gatgacacag agacaggcaa caaataccct     420

ggacaacctg gagcagacaa cagggagtgt ctgagtatgg actacaagca gacccaactt     480

tgtctgctgg gctgtaagcc tccaacagga gaacactggg gcaagggagt ggcttgtacc     540

aatgctgccc ctgccaatga ctgtcctcca ttggaactga taaacaccat cattgaggat     600

ggagatatgg tggacacagg ctttggctgt atggacttca agaccctcca agccaacaag     660

tctgatgtgc caattgacat ctgtggcagc acttgtaaat accctgacta cctgaaaatg     720

acctctgaac catatggaga ctccctgttc ttcttcctga ggagggaaca gatgtttgtg     780

agacacttct tcaacagggc tggcaccctg ggagaggctg tgcctgatga cctctacatc     840

aagggctctg gcaccacagc cagcatccag tcctctgcct tctttccaac accatctggc     900

agtatggtga cctctgagag ccaacttttc aacaagccat actggctcca aagggctcaa     960

ggacacaaca atggcatctg ttggggcaac caggtgtttg tgacagtggt ggacaccacc    1020

aggagcacca atatgaccct gtgtacccag gtgacctctg acagcaccta caagaatgag    1080

aacttcaagg aatacatcag gcatgtggag gaatatgacc tccaatttgt gttccaactt    1140

tgtaaggtga ccctgacagc agaggtgatg acctacatcc atgctatgaa ccctgacatc    1200

ttggaggact ggcagtttgg actgacacct cctccatctg cctccctcca agacacctac    1260

aggtttgtga ccagccaggc tatcacttgt cagaagacag tgcctccaaa ggagaaggag    1320

gacccactgg gcaaatacac cttctgggag gtggacctga aagagaagtt ctctgctgac    1380

ctggaccagt ttccactggg caggaagttc ctgctccaag caggactgaa agccaagcca    1440

aaactgaaaa gggctgcccc aaccagcacc aggacctcct ctgccaagag gaagaaggtg    1500

aagaagtaaa ctcgagctc                                                 1519


<210> 7
<211> 34
<212> DNA
<213> 人工序列


<220> 
<223> HPV16L1 F1

<400> 7
cttggtacca tgagtctgtg gctgccatct gagg                                 34


<210> 8
<211> 36
<212> DNA
<213> 人工序列


<220> 
<223> HPV16L1 R1

<400> 8
gcttggcttt cagtcctgct tggagcagga acttcc                               36


<210> 9
<211> 1441
<212> DNA
<213> 人工序列


<220> 
<223> HPV16L1扩增序列1

<400> 9
cttggtacca tgagtctgtg gctgccatct gaggctacag tctacctgcc tcctgtgcct      60

gtgagcaagg tggtgagcac agatgaatat gtggcaagga ccaacatcta ctaccatgct     120

ggcaccagca gactgctggc tgtgggacac ccatactttc caatcaagaa gccaaacaac     180

aacaagattc tggtgccaaa ggtgtctgga ctccaataca gggtgttcag gattcacctg     240

cctgacccaa acaagtttgg ctttcctgac acctccttct acaaccctga cacccagaga     300

ctggtgtggg cttgtgtggg agtggaggtg ggcaggggac aaccactggg agtgggcatc     360

tctggacacc cactgctgaa caaactggat gacacagaga atgcctctgc ctatgctgcc     420

aatgctggag tggacaacag ggagtgtatc agtatggact acaagcagac ccaactttgt     480

ctgattggct gtaagcctcc aattggagaa cactggggca agggcagccc atgtaccaat     540

gtggctgtga accctggaga ctgtcctcca ttggaactga taaacacagt gattcaggat     600

ggagatatgg tggacacagg ctttggagct atggacttca ccaccctcca agccaacaag     660

tctgaggtgc cactggacat ctgtaccagc atctgtaaat accctgacta catcaagatg     720

gtgtctgaac catatggaga ctccctgttc ttctacctga ggagggaaca gatgtttgtg     780

agacacctgt tcaacagggc tggagcagtg ggagagaatg tgcctgatga cctctacatc     840

aagggctctg gcagcacagc caacctggca tccagcaact actttccaac accatctggc     900

agtatggtga cctctgatgc ccagattttc aacaagccat actggctcca aagggctcaa     960

ggacacaaca atggcatctg ttggggcaac caactttttg tgacagtggt ggacaccacc    1020

aggagcacca atatgagtct gtgtgctgcc atcagcacct ctgagaccac ctacaagaac    1080

accaacttca aggaatacct gagacatgga gaggaatatg acctccaatt catcttccaa    1140

ctttgtaaga ttaccctgac agcagatgtg atgacctaca tccacagtat gaacagcacc    1200

atcttggagg actggaactt tggactccaa cctcctcctg gaggcacctt ggaggacacc    1260

tacaggtttg tgaccagcca ggctattgcc tgtcagaaac acacacctcc tgccccaaag    1320

gaggacccac tgaaaaaata caccttctgg gaggtgaacc tgaaagagaa gttctctgct    1380

gacctggacc agtttccact gggcaggaag ttcctgctcc aagcaggact gaaagccaag    1440

c                                                                    1441


<210> 10
<211> 35
<212> DNA
<213> 人工序列


<220> 
<223> HPV16L1 F2

<400> 10
agcaggactg aaagccaagc caaaactgaa aaggg                                35


<210> 11
<211> 36
<212> DNA
<213> 人工序列


<220> 
<223> HPV16L1 R2

<400> 11
ctgtctagat ttacttcttc accttcttcc tcttgg                               36


<210> 12
<211> 101
<212> DNA
<213> 人工序列


<220> 
<223> HPV16L1扩增序列2

<400> 12
agcaggactg aaagccaagc caaaactgaa aagggctgcc ccaaccagca ccaggacctc     60

ctctgccaag aggaagaagg tgaagaagta aatctagaca g                        101


<210> 13
<211> 38
<212> PRT
<213> 智人


<220> 
<223> HPV 59型L1蛋白的471-508氨基酸序列

<400> 13
Leu Gln Leu Gly Ala Arg Pro Lys Pro Thr Ile Gly Pro Arg Lys Arg 
1               5                   10                  15      
Ala Ala Pro Ala Pro Thr Ser Thr Pro Ser Pro Lys Arg Val Lys Arg 
            20                  25                  30          
Arg Lys Ser Ser Arg Lys 
        35              

