                         б

<110>  ϸ޹˾
 
<120>  һԴVEGFR2弰Ӧ

<130>  CIE200064PCT

<160>  76    

<170>  PatentIn version 3.5

<210>  1
<211>  764
<212>  PRT
<213>  

<400>  1

Met Gln Ser Lys Val Leu Leu Ala Val Ala Leu Trp Leu Cys Val Glu 
1               5                   10                  15      


Thr Arg Ala Ala Ser Val Gly Leu Pro Ser Val Ser Leu Asp Leu Pro 
            20                  25                  30          


Arg Leu Ser Ile Gln Lys Asp Ile Leu Thr Ile Lys Ala Asn Thr Thr 
        35                  40                  45              


Leu Gln Ile Thr Cys Arg Gly Gln Arg Asp Leu Asp Trp Leu Trp Pro 
    50                  55                  60                  


Asn Asn Gln Ser Gly Ser Glu Gln Arg Val Glu Val Thr Glu Cys Ser 
65                  70                  75                  80  


Asp Gly Leu Phe Cys Lys Thr Leu Thr Ile Pro Lys Val Ile Gly Asn 
                85                  90                  95      


Asp Thr Gly Ala Tyr Lys Cys Phe Tyr Arg Glu Thr Asp Leu Ala Ser 
            100                 105                 110         


Val Ile Tyr Val Tyr Val Gln Asp Tyr Arg Ser Pro Phe Ile Ala Ser 
        115                 120                 125             


Val Ser Asp Gln His Gly Val Val Tyr Ile Thr Glu Asn Lys Asn Lys 
    130                 135                 140                 


Thr Val Val Ile Pro Cys Leu Gly Ser Ile Ser Asn Leu Asn Val Ser 
145                 150                 155                 160 


Leu Cys Ala Arg Tyr Pro Glu Lys Arg Phe Val Pro Asp Gly Asn Arg 
                165                 170                 175     


Ile Ser Trp Asp Ser Lys Lys Gly Phe Thr Ile Pro Ser Tyr Met Ile 
            180                 185                 190         


Ser Tyr Ala Gly Met Val Phe Cys Glu Ala Lys Ile Asn Asp Glu Ser 
        195                 200                 205             


Tyr Gln Ser Ile Met Tyr Ile Val Val Val Val Gly Tyr Arg Ile Tyr 
    210                 215                 220                 


Asp Val Val Leu Ser Pro Ser His Gly Ile Glu Leu Ser Val Gly Glu 
225                 230                 235                 240 


Lys Leu Val Leu Asn Cys Thr Ala Arg Thr Glu Leu Asn Val Gly Ile 
                245                 250                 255     


Asp Phe Asn Trp Glu Tyr Pro Ser Ser Lys His Gln His Lys Lys Leu 
            260                 265                 270         


Val Asn Arg Asp Leu Lys Thr Gln Ser Gly Ser Glu Met Lys Lys Phe 
        275                 280                 285             


Leu Ser Thr Leu Thr Ile Asp Gly Val Thr Arg Ser Asp Gln Gly Leu 
    290                 295                 300                 


Tyr Thr Cys Ala Ala Ser Ser Gly Leu Met Thr Lys Lys Asn Ser Thr 
305                 310                 315                 320 


Phe Val Arg Val His Glu Lys Pro Phe Val Ala Phe Gly Ser Gly Met 
                325                 330                 335     


Glu Ser Leu Val Glu Ala Thr Val Gly Glu Arg Val Arg Ile Pro Ala 
            340                 345                 350         


Lys Tyr Leu Gly Tyr Pro Pro Pro Glu Ile Lys Trp Tyr Lys Asn Gly 
        355                 360                 365             


Ile Pro Leu Glu Ser Asn His Thr Ile Lys Ala Gly His Val Leu Thr 
    370                 375                 380                 


Ile Met Glu Val Ser Glu Arg Asp Thr Gly Asn Tyr Thr Val Ile Leu 
385                 390                 395                 400 


Thr Asn Pro Ile Ser Lys Glu Lys Gln Ser His Val Val Ser Leu Val 
                405                 410                 415     


Val Tyr Val Pro Pro Gln Ile Gly Glu Lys Ser Leu Ile Ser Pro Val 
            420                 425                 430         


Asp Ser Tyr Gln Tyr Gly Thr Thr Gln Thr Leu Thr Cys Thr Val Tyr 
        435                 440                 445             


Ala Ile Pro Pro Pro His His Ile His Trp Tyr Trp Gln Leu Glu Glu 
    450                 455                 460                 


Glu Cys Ala Asn Glu Pro Ser Gln Ala Val Ser Val Thr Asn Pro Tyr 
465                 470                 475                 480 


Pro Cys Glu Glu Trp Arg Ser Val Glu Asp Phe Gln Gly Gly Asn Lys 
                485                 490                 495     


Ile Glu Val Asn Lys Asn Gln Phe Ala Leu Ile Glu Gly Lys Asn Lys 
            500                 505                 510         


Thr Val Ser Thr Leu Val Ile Gln Ala Ala Asn Val Ser Ala Leu Tyr 
        515                 520                 525             


Lys Cys Glu Ala Val Asn Lys Val Gly Arg Gly Glu Arg Val Ile Ser 
    530                 535                 540                 


Phe His Val Thr Arg Gly Pro Glu Ile Thr Leu Gln Pro Asp Met Gln 
545                 550                 555                 560 


Pro Thr Glu Gln Glu Ser Val Ser Leu Trp Cys Thr Ala Asp Arg Ser 
                565                 570                 575     


Thr Phe Glu Asn Leu Thr Trp Tyr Lys Leu Gly Pro Gln Pro Leu Pro 
            580                 585                 590         


Ile His Val Gly Glu Leu Pro Thr Pro Val Cys Lys Asn Leu Asp Thr 
        595                 600                 605             


Leu Trp Lys Leu Asn Ala Thr Met Phe Ser Asn Ser Thr Asn Asp Ile 
    610                 615                 620                 


Leu Ile Met Glu Leu Lys Asn Ala Ser Leu Gln Asp Gln Gly Asp Tyr 
625                 630                 635                 640 


Val Cys Leu Ala Gln Asp Arg Lys Thr Lys Lys Arg His Cys Val Val 
                645                 650                 655     


Arg Gln Leu Thr Val Leu Glu Arg Val Ala Pro Thr Ile Thr Gly Asn 
            660                 665                 670         


Leu Glu Asn Gln Thr Thr Ser Ile Gly Glu Ser Ile Glu Val Ser Cys 
        675                 680                 685             


Thr Ala Ser Gly Asn Pro Pro Pro Gln Ile Met Trp Phe Lys Asp Asn 
    690                 695                 700                 


Glu Thr Leu Val Glu Asp Ser Gly Ile Val Leu Lys Asp Gly Asn Arg 
705                 710                 715                 720 


Asn Leu Thr Ile Arg Arg Val Arg Lys Glu Asp Glu Gly Leu Tyr Thr 
                725                 730                 735     


Cys Gln Ala Cys Ser Val Leu Gly Cys Ala Lys Val Glu Ala Phe Phe 
            740                 745                 750         


Ile Ile Glu Gly Ala Gln Glu Lys Thr Asn Leu Glu 
        755                 760                 


<210>  2
<211>  54
<212>  DNA
<213>  ˹

<220>
<223>  ɾ忹ʱscFvĽͷ

<400>  2
tctagtggtg gcggtggttc gggcggtggt ggaggtggta gttctagatc ttcc             54


<210>  3
<211>  726
<212>  DNA
<213>  ˹

<220>
<223>  VEGFR2-MS76 scFv

<400>  3
gacatccaga tgactcagac tccatccagt ctgtctgcat cccttggaga cacaattacc       60

atcacttgcc atgccagtca gaacattaat gtttggttaa gctggtacca gcagaaacca      120

ggaaatattc ctaaactatt gatctataag gcttccaact tgcacacagg cgtcccatca      180

aggtttagtg gcagtggatc tggaacaggt ttcacattaa ccatcagcag cctgcagcct      240

gaagacattg ccacttacta ctgtcaacag ggtcaaagtt atcctctgac gttcggtgga      300

ggcaccaagc tggaaatcaa atctagtggt ggcggtggtt cgggcggtgg tggaggtggt      360

agttctagat cttccgaggt ccagctgcag cagtctggac ctgaactggt gaagcctggg      420

gcttcagtga aggtatcctg caaggcttct ggttactcat tcactgacta caccatgtac      480

tgggtgaagc agagccatgg aaagagcctt gagtggattg gatatattga tccttacaat      540

ggtgatacta gctacaacca gaagttcaag ggcaaggcca cattgactgt tgacaagtcc      600

tccagcacag ccttcatgca tctcaacagc ctgacatctg aggactctgc agtctattac      660

tgtgcaagag gatactacga tgctatggcc tactggggtc aaggaacctc agtcaccgtc      720

tcctca                                                                 726


<210>  4
<211>  351
<212>  DNA
<213>  С

<400>  4
gaggtccagc tgcagcagtc tggacctgaa ctggtgaagc ctggggcttc agtgaaggta       60

tcctgcaagg cttctggtta ctcattcact gactacacca tgtactgggt gaagcagagc      120

catggaaaga gccttgagtg gattggatat attgatcctt acaatggtga tactagctac      180

aaccagaagt tcaagggcaa ggccacattg actgttgaca agtcctccag cacagccttc      240

atgcatctca acagcctgac atctgaggac tctgcagtct attactgtgc aagaggatac      300

tacgatgcta tggcctactg gggtcaagga acctcagtca ccgtctcctc a               351


<210>  5
<211>  321
<212>  DNA
<213>  С

<400>  5
gacatccaga tgactcagac tccatccagt ctgtctgcat cccttggaga cacaattacc       60

atcacttgcc atgccagtca gaacattaat gtttggttaa gctggtacca gcagaaacca      120

ggaaatattc ctaaactatt gatctataag gcttccaact tgcacacagg cgtcccatca      180

aggtttagtg gcagtggatc tggaacaggt ttcacattaa ccatcagcag cctgcagcct      240

gaagacattg ccacttacta ctgtcaacag ggtcaaagtt atcctctgac gttcggtgga      300

ggcaccaagc tggaaatcaa a                                                321


<210>  6
<211>  996
<212>  DNA
<213>  

<400>  6
gctagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg       60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc      300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga      360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct      420

gaggtcacgt gcgtggtggt ggacgtgagc cacgaagacc ccgaggtcaa gttcaactgg      480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac      540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag      600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc      660

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag      720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc      780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg      840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg      900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacc      960

cagaagtccc tgtctctgag ccctggctaa tagtga                                996


<210>  7
<211>  324
<212>  DNA
<213>  

<400>  7
cgtacggtgg ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct       60

ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag      120

tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac      180

agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag      240

aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag      300

agcttcaaca ggggagagtg ttag                                             324


<210>  8
<211>  117
<212>  PRT
<213>  С

<400>  8

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Tyr Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Phe 
65                  70                  75                  80  


Met His Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Tyr Asp Ala Met Ala Tyr Trp Gly Gln Gly Thr Ser 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  9
<211>  107
<212>  PRT
<213>  С

<400>  9

Asp Ile Gln Met Thr Gln Thr Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Thr Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  10
<211>  11
<212>  PRT
<213>  С

<400>  10

His Ala Ser Gln Asn Ile Asn Val Trp Leu Ser 
1               5                   10      


<210>  11
<211>  7
<212>  PRT
<213>  С

<400>  11

Lys Ala Ser Asn Leu His Thr 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  С

<400>  12

Gln Gln Gly Gln Ser Tyr Pro Leu Thr 
1               5                   


<210>  13
<211>  10
<212>  PRT
<213>  С

<400>  13

Gly Tyr Ser Phe Thr Asp Tyr Thr Met Tyr 
1               5                   10  


<210>  14
<211>  17
<212>  PRT
<213>  С

<400>  14

Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  15
<211>  10
<212>  PRT
<213>  С

<400>  15

Ala Arg Gly Tyr Tyr Asp Ala Met Ala Tyr 
1               5                   10  


<210>  16
<211>  446
<212>  PRT
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Tyr Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Tyr Asp Ala Met Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
        435                 440                 445     


<210>  17
<211>  214
<212>  PRT
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76

<400>  17

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  18
<211>  465
<212>  PRT
<213>  ˹

<220>
<223>  źĵԴVEGFR2-HS76

<400>  18

Met Gly Trp Ser Leu Ile Leu Leu Phe Leu Val Ala Val Ala Thr Arg 
1               5                   10                  15      


Val Leu Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe 
        35                  40                  45              


Thr Asp Tyr Thr Met Tyr Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Arg Asp Thr Ser Ile Ser 
                85                  90                  95      


Thr Ala Tyr Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Tyr Tyr Asp Ala Met Ala Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
    130                 135                 140                 


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
145                 150                 155                 160 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
                165                 170                 175     


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
            180                 185                 190         


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
        195                 200                 205             


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
    210                 215                 220                 


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
225                 230                 235                 240 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                245                 250                 255     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            260                 265                 270         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        275                 280                 285             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    290                 295                 300                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
305                 310                 315                 320 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                325                 330                 335     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            340                 345                 350         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
        355                 360                 365             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    370                 375                 380                 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
385                 390                 395                 400 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                405                 410                 415     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
            420                 425                 430         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        435                 440                 445             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    450                 455                 460                 


Gly 
465 


<210>  19
<211>  233
<212>  PRT
<213>  ˹

<220>
<223>  źĵԴVEGFR2-HS76

<400>  19

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala 
            20                  25                  30          


Ser Val Gly Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile 
        35                  40                  45              


Asn Val Trp Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys 
    50                  55                  60                  


Leu Leu Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                85                  90                  95      


Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser 
            100                 105                 110         


Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Thr 
        115                 120                 125             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    130                 135                 140                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
145                 150                 155                 160 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                165                 170                 175     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            180                 185                 190         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        195                 200                 205             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    210                 215                 220                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230             


<210>  20
<211>  19
<212>  PRT
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76źİ

<400>  20

Met Gly Trp Ser Leu Ile Leu Leu Phe Leu Val Ala Val Ala Thr Arg 
1               5                   10                  15      


Val Leu Ser 
            


<210>  21
<211>  19
<212>  PRT
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76źİ

<400>  21

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  22
<211>  117
<212>  PRT
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76ɱ

<400>  22

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr 
            20                  25                  30          


Thr Met Tyr Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Tyr Tyr Asp Ala Met Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  23
<211>  107
<212>  PRT
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76ɱ

<400>  23

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  24
<211>  329
<212>  PRT
<213>  

<400>  24

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  25
<211>  107
<212>  PRT
<213>  

<400>  25

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  26
<211>  1404
<212>  DNA
<213>  ˹

<220>
<223>  źĵԴVEGFR2-HS76

<400>  26
atgggctggt ccctgattct gctgttcctg gtggctgtgg ctaccagggt gctgagtcag       60

gtccaacttg tccagtctgg agcagaggtg aagaagcctg gagcctctgt gaaggtgtcc      120

tgtaaggcat ctggctactc cttcacagac tacacaatgt attgggtgag acaggctcct      180

ggacaaggat tggagtggat tggctacatt gacccataca atggagacac ctcctacaac      240

cagaagttca agggcaaggc taccctgacc agggacacca gcatcagcac agcctatatg      300

gaactgagca gactgaggtc tgatgacaca gcagtctact actgtgccag gggctactat      360

gatgctatgg cttactgggg acaaggcacc ctggtgacag tgtccagcgc aagcaccaag      420

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc      480

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc      540

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc      600

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac      660

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac      720

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      780

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacgtgc      840

gtggtggtgg acgtgagcca cgaagacccc gaggtcaagt tcaactggta cgtggacggc      900

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      960

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1020

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1080

cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac     1140

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1200

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1260

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1320

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacaccca gaagtccctg     1380

tctctgagcc ctggctaata gtga                                            1404


<210>  27
<211>  702
<212>  DNA
<213>  ˹

<220>
<223>  źĵԴVEGFR2-HS76

<400>  27
atgggctggt cctgtatcat cctgttcctg gtggctacag ccacaggagt gcatagtgac       60

atccagatga cccagagccc atcctccctg tctgcctctg tgggagacag ggtgaccatc      120

acttgtcatg ccagccagaa catcaatgtg tggctgtcct ggtatcaaca gaagcctggc      180

aacatcccaa aactgctgat ttacaaggca agcaacctgc acacaggagt gccaagcagg      240

ttctctggct ctggctctgg cacagacttc accctgacca tctcctccct ccaacctgag      300

gacattgcca cctactactg tcaacaggga caatcctacc cactgacctt tggaggaggc      360

accaaggtgg agattaagcg tacggtggct gcaccatctg tcttcatctt cccgccatct      420

gatgagcagt tgaaatctgg aactgcctct gttgtgtgcc tgctgaataa cttctatccc      480

agagaggcca aagtacagtg gaaggtggat aacgccctcc aatcgggtaa ctcccaggag      540

agtgtcacag agcaggacag caaggacagc acctacagcc tcagcagcac cctgacgctg      600

agcaaagcag actacgagaa acacaaagtc tacgcctgcg aagtcaccca tcagggcctg      660

agctcgcccg tcacaaagag cttcaacagg ggagagtgtt ag                         702


<210>  28
<211>  57
<212>  DNA
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76źĺ

<400>  28
atgggctggt ccctgattct gctgttcctg gtggctgtgg ctaccagggt gctgagt          57


<210>  29
<211>  57
<212>  DNA
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76źĺ

<400>  29
atgggctggt cctgtatcat cctgttcctg gtggctacag ccacaggagt gcatagt          57


<210>  30
<211>  351
<212>  DNA
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76ɱ

<400>  30
caggtccaac ttgtccagtc tggagcagag gtgaagaagc ctggagcctc tgtgaaggtg       60

tcctgtaagg catctggcta ctccttcaca gactacacaa tgtattgggt gagacaggct      120

cctggacaag gattggagtg gattggctac attgacccat acaatggaga cacctcctac      180

aaccagaagt tcaagggcaa ggctaccctg accagggaca ccagcatcag cacagcctat      240

atggaactga gcagactgag gtctgatgac acagcagtct actactgtgc caggggctac      300

tatgatgcta tggcttactg gggacaaggc accctggtga cagtgtccag c               351


<210>  31
<211>  321
<212>  DNA
<213>  ˹

<220>
<223>  ԴVEGFR2-HS76ɱ

<400>  31
gacatccaga tgacccagag cccatcctcc ctgtctgcct ctgtgggaga cagggtgacc       60

atcacttgtc atgccagcca gaacatcaat gtgtggctgt cctggtatca acagaagcct      120

ggcaacatcc caaaactgct gatttacaag gcaagcaacc tgcacacagg agtgccaagc      180

aggttctctg gctctggctc tggcacagac ttcaccctga ccatctcctc cctccaacct      240

gaggacattg ccacctacta ctgtcaacag ggacaatcct acccactgac ctttggagga      300

ggcaccaagg tggagattaa g                                                321


<210>  32
<211>  996
<212>  DNA
<213>  

<400>  32
gcaagcacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg       60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc      300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga      360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct      420

gaggtcacgt gcgtggtggt ggacgtgagc cacgaagacc ccgaggtcaa gttcaactgg      480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac      540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag      600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc      660

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggatgag      720

ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc      780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg      840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg      900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacc      960

cagaagtccc tgtctctgag ccctggctaa tagtga                                996


<210>  33
<211>  242
<212>  PRT
<213>  ˹

<220>
<223>  VEGFR2-MS76scFv

<400>  33

Asp Ile Gln Met Thr Gln Thr Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Thr Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Tyr Pro Leu 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Ser Ser Gly Gly Gly 
            100                 105                 110         


Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg Ser Ser Glu Val Gln 
        115                 120                 125             


Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Val Lys 
    130                 135                 140                 


Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Asp Tyr Thr Met Tyr 
145                 150                 155                 160 


Trp Val Lys Gln Ser His Gly Lys Ser Leu Glu Trp Ile Gly Tyr Ile 
                165                 170                 175     


Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe Lys Gly Lys 
            180                 185                 190         


Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Phe Met His Leu 
        195                 200                 205             


Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Gly 
    210                 215                 220                 


Tyr Tyr Asp Ala Met Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser 
        


<210>  34
<211>  18
<212>  PRT
<213>  ˹

<220>
<223>  ɾ忹õscFvͷİ

<400>  34

Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Gly Gly Ser Ser Arg 
1               5                   10                  15      


Ser Ser 
        


<210>  35
<211>  1405
<212>  DNA
<213>  ˹

<220>
<223>  źĵǶϿVEGFR2-mhS76

<400>  35
atgggctggt ccctgattct gctgttcctg gtggctgtgg ctaccagggt gctgagtgag       60

gtccagctgc agcagtctgg acctgaactg gtgaagcctg gggcttcagt gaaggtatcc      120

tgcaaggctt ctggttactc attcactgac tacaccatgt actgggtgaa gcagagccat      180

ggaaagagcc ttgagtggat tggatatatt gatccttaca atggtgatac tagctacaac      240

cagaagttca agggcaaggc cacattgact gttgacaagt cctccagcac agccttcatg      300

catctcaaca gcctgacatc tgaggactct gcagtctatt actgtgcaag aggatactac      360

gatgctatgg cctactgggg tcaaggaacc tcagtcaccg tctcctcagc tagcaccaag      420

ggcccatcgg tcttccccct ggcaccctcc tccaagagca cctctggggg cacagcggcc      480

ctgggctgcc tggtcaagga ctacttcccc gaaccggtga cggtgtcgtg gaactcaggc      540

gccctgacca gcggcgtgca caccttcccg gctgtcctac agtcctcagg actctactcc      600

ctcagcagcg tggtgaccgt gccctccagc agcttgggca cccagaccta catctgcaac      660

gtgaatcaca agcccagcaa caccaaggtg gacaagaaag ttgagcccaa atcttgtgac      720

aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc      780

ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacgtgc      840

gtggtggtgg acgtgagcca cgaagacccc gaggtcaagt tcaactggta cgtggacggc      900

gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt      960

gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc     1020

aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg     1080

cagccccgag aaccacaggt gtacaccctg cccccatccc gggatgagct gaccaagaac     1140

caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg     1200

gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac     1260

ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac     1320

gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacaccca gaagtccctg     1380

tctctgagcc ctggctaata gtgaa                                           1405


<210>  36
<211>  702
<212>  DNA
<213>  ˹

<220>
<223>  źĵǶϿVEGFR2-mHS76

<400>  36
atgggctggt cctgtatcat cctgttcctg gtggctacag ccacaggagt gcatagtgac       60

atccagatga ctcagactcc atccagtctg tctgcatccc ttggagacac aattaccatc      120

acttgccatg ccagtcagaa cattaatgtt tggttaagct ggtaccagca gaaaccagga      180

aatattccta aactattgat ctataaggct tccaacttgc acacaggcgt cccatcaagg      240

tttagtggca gtggatctgg aacaggtttc acattaacca tcagcagcct gcagcctgaa      300

gacattgcca cttactactg tcaacagggt caaagttatc ctctgacgtt cggtggaggc      360

accaagctgg aaatcaaacg tacggtggct gcaccatctg tcttcatctt cccgccatct      420

gatgagcagt tgaaatctgg aactgcctct gttgtgtgcc tgctgaataa cttctatccc      480

agagaggcca aagtacagtg gaaggtggat aacgccctcc aatcgggtaa ctcccaggag      540

agtgtcacag agcaggacag caaggacagc acctacagcc tcagcagcac cctgacgctg      600

agcaaagcag actacgagaa acacaaagtc tacgcctgcg aagtcaccca tcagggcctg      660

agctcgcccg tcacaaagag cttcaacagg ggagagtgtt ag                         702


<210>  37
<211>  465
<212>  PRT
<213>  ˹

<220>
<223>  źĵǶϿVEGFR2-mhS76

<400>  37

Met Gly Trp Ser Leu Ile Leu Leu Phe Leu Val Ala Val Ala Thr Arg 
1               5                   10                  15      


Val Leu Ser Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Ser Phe 
        35                  40                  45              


Thr Asp Tyr Thr Met Tyr Trp Val Lys Gln Ser His Gly Lys Ser Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Ile Asp Pro Tyr Asn Gly Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Phe Met His Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Gly Tyr Tyr Asp Ala Met Ala Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Ser Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
    130                 135                 140                 


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
145                 150                 155                 160 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
                165                 170                 175     


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
            180                 185                 190         


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
        195                 200                 205             


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
    210                 215                 220                 


Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp 
225                 230                 235                 240 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
                245                 250                 255     


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
            260                 265                 270         


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
        275                 280                 285             


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
    290                 295                 300                 


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
305                 310                 315                 320 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
                325                 330                 335     


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
            340                 345                 350         


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
        355                 360                 365             


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
    370                 375                 380                 


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
385                 390                 395                 400 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
                405                 410                 415     


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
            420                 425                 430         


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
        435                 440                 445             


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
    450                 455                 460                 


Gly 
465 


<210>  38
<211>  233
<212>  PRT
<213>  ˹

<220>
<223>  źĵǶϿVEGFR2-mhS76

<400>  38

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Asp Ile Gln Met Thr Gln Thr Pro Ser Ser Leu Ser Ala 
            20                  25                  30          


Ser Leu Gly Asp Thr Ile Thr Ile Thr Cys His Ala Ser Gln Asn Ile 
        35                  40                  45              


Asn Val Trp Leu Ser Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys 
    50                  55                  60                  


Leu Leu Ile Tyr Lys Ala Ser Asn Leu His Thr Gly Val Pro Ser Arg 
65                  70                  75                  80  


Phe Ser Gly Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser 
                85                  90                  95      


Leu Gln Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser 
            100                 105                 110         


Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Thr 
        115                 120                 125             


Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu 
    130                 135                 140                 


Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro 
145                 150                 155                 160 


Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly 
                165                 170                 175     


Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr 
            180                 185                 190         


Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His 
        195                 200                 205             


Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val 
    210                 215                 220                 


Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230             


<210>  39
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  ɱF1

<400>  39
gctaccaggg tgctgagtga ggtccagctg cagcag                                 36


<210>  40
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  ɱR1

<400>  40
tgggcccttg gtgctagctg aggagacggt gactga                                 36


<210>  41
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  ɱF2

<400>  41
gccacaggag tgcatagtga catccagatg actcag                                 36


<210>  42
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  ɱR2

<400>  42
tggtgcagcc accgtacgtt tgatttccag cttggt                                 36


<210>  43
<211>  51
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF3

<400>  43
gctaccaggg tgctgagtca ggtccaactt gtccagtctg gagcagaggt g                51


<210>  44
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR3

<400>  44
cacagaggct ccaggcttct tcacctctgc tcc                                    33


<210>  45
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF4

<400>  45
cctggagcct ctgtgaaggt gtcctgtaag gca                                    33


<210>  46
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR4

<400>  46
gtctgtgaag gagtagccag atgccttaca gga                                    33


<210>  47
<211>  30
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF5

<400>  47
tactccttca cagactacac aatgtattgg                                        30


<210>  48
<211>  31
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR5

<400>  48
gtccaggagc ctgtctcacc caatacattg t                                      31


<210>  49
<211>  30
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF6

<400>  49
gacaggctcc tggacaagga ttggagtgga                                        30


<210>  50
<211>  31
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR6

<400>  50
tatgggtcaa tgtagccaat ccactccaat c                                      31


<210>  51
<211>  30
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF7

<400>  51
ctacattgac ccatacaatg gagacacctc                                        30


<210>  52
<211>  31
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR7

<400>  52
cttgaacttc tggttgtagg aggtgtctcc a                                      31


<210>  53
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF8

<400>  53
aaccagaagt tcaagggcaa ggctaccctg acc                                    33


<210>  54
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR8

<400>  54
tgtgctgatg ctggtgtccc tggtcagggt agc                                    33


<210>  55
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF9

<400>  55
accagcatca gcacagccta tatggaactg agc                                    33


<210>  56
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR9

<400>  56
tgtgtcatca gacctcagtc tgctcagttc cat                                    33


<210>  57
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF10

<400>  57
aggtctgatg acacagcagt ctactactgt gcc                                    33


<210>  58
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR10

<400>  58
catagcatca tagtagcccc tggcacagta gta                                    33


<210>  59
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF11

<400>  59
tactatgatg ctatggctta ctggggacaa ggc                                    33


<210>  60
<211>  51
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR11

<400>  60
tgggcccttg gtgcttgcgc tggacactgt caccagggtg ccttgtcccc a                51


<210>  61
<211>  51
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF12

<400>  61
gccacaggag tgcatagtga catccagatg acccagagcc catcctccct g                51


<210>  62
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR12

<400>  62
cctgtctccc acagaggcag acagggagga tgg                                    33


<210>  63
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF13

<400>  63
tctgtgggag acagggtgac catcacttgt cat                                    33


<210>  64
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR13

<400>  64
cacattgatg ttctggctgg catgacaagt gat                                    33


<210>  65
<211>  32
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF14

<400>  65
cagaacatca atgtgtggct gtcctggtat ca                                     32


<210>  66
<211>  32
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR14

<400>  66
ggatgttgcc aggcttctgt tgataccagg ac                                     32


<210>  67
<211>  32
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF15

<400>  67
agcctggcaa catcccaaaa ctgctgattt ac                                     32


<210>  68
<211>  32
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR15

<400>  68
gtgtgcaggt tgcttgcctt gtaaatcagc ag                                     32


<210>  69
<211>  32
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF16

<400>  69
aagcaacctg cacacaggag tgccaagcag gt                                     32


<210>  70
<211>  32
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR16

<400>  70
gccagagcca gagccagaga acctgcttgg ca                                     32


<210>  71
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF17

<400>  71
ggctctggct ctggcacaga cttcaccctg acc                                    33


<210>  72
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR17

<400>  72
ctcaggttgg agggaggaga tggtcagggt gaa                                    33


<210>  73
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF18

<400>  73
tccctccaac ctgaggacat tgccacctac tac                                    33


<210>  74
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR18

<400>  74
gtaggattgt ccctgttgac agtagtaggt ggc                                    33


<210>  75
<211>  33
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱF19

<400>  75
cagggacaat cctacccact gacctttgga gga                                    33


<210>  76
<211>  51
<212>  DNA
<213>  ˹

<220>
<223>  ȫϳɱR19

<400>  76
tggtgcagcc accgtacgct taatctccac cttggtgcct cctccaaagg t                51


