                         SEQUENCE LISTING

<110>  National University of Singapore
       Beth Israel Deaconess Medical Center, Inc.
 
<120>  METHODS AND COMPOSITIONS FOR GENE SPECIFIC DEMETHYLATION AND 
       ACTIVATION

<130>  08942729WO

<150>  US 62/874,160
<151>  2019-07-15

<160>  113   

<170>  PatentIn version 3.5

<210>  1
<211>  139
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G19sgR2R5

<400>  1
gcucccccgc cugccagcaa guuugagagc uacccgggac gcggguccgg gacaguagca       60

aguucaaaua aggcuagucc guuaucaacu ucugaggccu uggcgaggcu ucuaaguggc      120

accgagucgg ugcuuuuuu                                                   139


<210>  2
<211>  140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G36sgR2R5

<400>  2
gcuaacugcc aaauugaauc gguuugagag cuacccggga cgcggguccg ggacaguagc       60

aaguucaaau aaggcuaguc cguuaucaac uucugaggcc uuggcgaggc uucuaagugg      120

caccgagucg gugcuuuuuu                                                  140


<210>  3
<211>  139
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G110sgR2R5

<400>  3
gacccucuac ccaccuggau guuugagagc uacccgggac gcggguccgg gacaguagca       60

aguucaaaua aggcuagucc guuaucaacu ucugaggccu uggcgaggcu ucuaaguggc      120

accgagucgg ugcuuuuuu                                                   139


<210>  4
<211>  139
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G111sgR2R5

<400>  4
gcccccaggg cgucgccagg guuugagagc uacccgggac gcggguccgg gacaguagca       60

aguucaaaua aggcuagucc guuaucaacu ucugaggccu uggcgaggcu ucuaaguggc      120

accgagucgg ugcuuuuuu                                                   139


<210>  5
<211>  139
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G108sgR2R5

<400>  5
guggccagcc agucagccga guuugagagc uacccgggac gcggguccgg gacaguagca       60

aguucaaaua aggcuagucc guuaucaacu ucugaggccu uggcgaggcu ucuaaguggc      120

accgagucgg ugcuuuuuu                                                   139


<210>  6
<211>  140
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G122sgR2R5

<400>  6
gccgcagccg ccgagcgcac gguuugagag cuacccggga cgcggguccg ggacaguagc       60

aaguucaaau aaggcuaguc cguuaucaac uucugaggcc uuggcgaggc uucuaagugg      120

caccgagucg gugcuuuuuu                                                  140


<210>  7
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R2 stem loop of DiR

<400>  7
cccgggacgc ggguccggga cag                                               23


<210>  8
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5 stem loop of DiR

<400>  8
cugaggccuu ggcgaggcuu cu                                                22


<210>  9
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  P16 targeting portion example

<400>  9
gcucccccgc cugccagcaa                                                   20


<210>  10
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  P16 targeting portion example

<400>  10
gcuaacugcc aaauugaauc g                                                 21


<210>  11
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  P16 targeting portion example

<400>  11
gacccucuac ccaccuggau                                                   20


<210>  12
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  P16 targeting portion example

<400>  12
gcccccaggg cgucgccagg                                                   20


<210>  13
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Guide 1.6 sgDiR

<400>  13
gcugcggcug cugcucgccc                                                   20


<210>  14
<211>  5040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dCas9-mCherry Plasmid

<400>  14
atggactata aggaccacga cggagactac aaggatcatg atattgatta caaagacgat       60

gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc      120

gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc      180

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac      240

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc      300

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat      360

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg      420

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac      480

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa      540

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg      600

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg      660

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc      720

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg      780

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg      840

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat      900

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag      960

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg     1020

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg     1080

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag     1140

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc     1200

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa     1260

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag     1320

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc     1380

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag     1440

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga     1500

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg     1560

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac     1620

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat     1680

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc     1740

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg     1800

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc     1860

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc     1920

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg     1980

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac     2040

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg     2100

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat     2160

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc     2220

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac     2280

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg     2340

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc     2400

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg     2460

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg     2520

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat     2580

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggacgccatc     2640

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac     2700

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac     2760

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc     2820

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg     2880

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact     2940

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag     3000

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac     3060

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac     3120

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3180

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac     3240

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct     3300

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc     3360

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag     3420

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc     3480

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat     3540

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa     3600

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt     3660

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac     3720

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag     3780

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac     3840

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag     3900

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc     3960

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc     4020

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct     4080

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag     4140

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac     4200

ctgtctcagc tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa     4260

aagaaaaagg gcggtgaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat     4320

cctggcccaa tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg     4380

cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag     4440

ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc     4500

cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac     4560

gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag     4620

tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc     4680

ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac     4740

ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc     4800

gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac     4860

tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc     4920

tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa     4980

cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag     5040


<210>  15
<211>  139
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgRNA

<400>  15
gcacucaaac acgccuuugc guuuuagagc uacccgggac gcggguccgg gacaguagca       60

aguuaaaaua aggcuagucc guuaucaacu ucugaggccu uggcgaggcu ucuaaguggc      120

accgagucgg ugcuuuuuu                                                   139


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16 Forward Primer

<400>  16
ccccttgcct ggaaagatac                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16 Reverse Primer

<400>  17
agcccctcct ctttcttcct                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16 Forward Primer

<400>  18
caacgcaccg aatagttacg                                                   20


<210>  19
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16 Reverse Primer

<400>  19
agcaccacca gcgtgtc                                                      17


<210>  20
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  GN2 Guide Sequence

<400>  20
guuaggaaua aaagcuuuga                                                   20


<210>  21
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>   G100 Guide Sequence

<400>  21
gugaaccgag agagaucgug                                                   20


<210>  22
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G101 Guide Sequence

<400>  22
gcccccauua agaaccacug u                                                 21


<210>  23
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G102 Guide Sequence

<400>  23
gguugccagg augggaggga                                                   20


<210>  24
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G103 Guide Sequence

<400>  24
guucuucuca aaaaagaaag u                                                 21


<210>  25
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G25 Guide Sequence

<400>  25
gacaggacag uauuugaagc                                                   20


<210>  26
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G126 Guide Sequence

<400>  26
gguuuauuua auacggacgg                                                   20


<210>  27
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G127 Guide Sequence

<400>  27
gacagccguu uuacacgcag g                                                 21


<210>  28
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G128 Guide Sequence

<400>  28
gcaggugauu ucgauucucg g                                                 21


<210>  29
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G2 Guide Sequence

<400>  29
gcacucaaac acgccuuugc                                                   20


<210>  30
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G82 Guide Sequence

<400>  30
guaucgcgga ggaaggaaac g                                                 21


<210>  31
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G107 Guide Sequence

<400>  31
gcauggagcc uucggcugac                                                   20


<210>  32
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G108 Guide Se

<400>  32
guggccagcc agucagccga                                                   20


<210>  33
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G122 Guide Sequence

<400>  33
gccgcagccg ccgagcgcac g                                                 21


<210>  34
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G123 Guide Sequence

<400>  34
gaggggcugg cuggucacca g                                                 21


<210>  35
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G109 Guide Sequence

<400>  35
gcaccgaaua guuacggucg g                                                 21


<210>  36
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G112 Guide Sequence

<400>  36
gaaaaagggg aggcuuccug                                                   20


<210>  37
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G113 Guide Sequence

<400>  37
ggauuaucag uggaaaucug                                                   20


<210>  38
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G114 Guide Sequence

<400>  38
gaaagaaaug uaagaugugc u                                                 21


<210>  39
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G115 Guide Sequence

<400>  39
gaagaaagau aagcuccauc c                                                 21


<210>  40
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G116 Guide Sequence

<400>  40
gugaagggau uacaaggcgu g                                                 21


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16 Forward Primer

<400>  41
caacgcaccg aatagttacg                                                   20


<210>  42
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16 Reverse Primer

<400>  42
agcaccacca gcgtgtc                                                      17


<210>  43
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R2 Sequence

<400>  43
cccgggacgc ggguccggga cag                                               23


<210>  44
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  R5 Sequence

<400>  44
cugaggccuu ggcgaggcuu cu                                                22


<210>  45
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Original sgRNA Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  45
nnnnnnnnnn nnnnnnnnng uuuuagagcu agaaauagca aguuaaaaua aggcuagucc       60

guuaucaacu ugaaaaagug gcaccgaguc ggugcuuuuu                            100


<210>  46
<211>  100
<212>  RNA
<213>  Artificial Sequence

<220>
<223>   sgRNA Sequence Revision


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  46
nnnnnnnnnn nnnnnnnnng uuugagagcu agaaauagca aguucaaaua aggcuagucc       60

guuaucaacu ugaaaaagug gcaccgaguc ggugcuuuuu                            100


<210>  47
<211>  160
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  sgSAM Comparison Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  47
nnnnnnnnnn nnnnnnnnng uuuuagagcu aggccaacau gaggaucacc caugucugca       60

gggccuagca aguuaaaaua aggcuagucc guuaucaacu uggccaacau gaggaucacc      120

caugucugca gggccaagug gcaccgaguc ggugcuuuuu                            160


<210>  48
<211>  119
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR1 R2-Stemloop2 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  48
nnnnnnnnnn nnnnnnnnng uuugagagcu acccgggacg cggguccggg acaguagcaa       60

guucaaauaa ggcuaguccg uuaucaacuu gaaaaagugg caccgagucg gugcuuuuu       119


<210>  49
<211>  118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR2 R5-Stemloop2 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  49
nnnnnnnnnn nnnnnnnnng uuugagagcu acugaggccu uggcgaggcu ucuuagcaag       60

uucaaauaag gcuaguccgu uaucaacuug aaaaaguggc accgagucgg ugcuuuuu        118


<210>  50
<211>  118
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR3 Tetraloop-R2 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  50
nnnnnnnnnn nnnnnnnnng uuugagagcu agaaauagca aguucaaaua aggcuagucc       60

guuaucaacu ucccgggacg cggguccggg acagaguggc accgagucgg ugcuuuuu        118


<210>  51
<211>  117
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR4 Tetraloop-R5 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  51
nnnnnnnnnn nnnnnnnnng uuugagagcu agaaauagca aguucaaaua aggcuagucc       60

guuaucaacu ucugaggccu uggcgaggcu ucuaguggca ccgagucggu gcuuuuu         117


<210>  52
<211>  138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR5 R5-R2 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  52
nnnnnnnnnn nnnnnnnnng uuugagagcu acugaggccu uggcgaggcu ucuuagcaag       60

uucaaauaag gcuaguccgu uaucaacuuc ccgggacgcg gguccgggac agaaguggca      120

ccgagucggu gcuuuuuu                                                    138


<210>  53
<211>  138
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR6 R2-R5 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  53
nnnnnnnnnn nnnnnnnnng uuugagagcu acccgggacg cggguccggg acaguagcaa       60

guucaaauaa ggcuaguccg uuaucaacuu cugaggccuu ggcgaggcuu cuaaguggca      120

ccgagucggu gcuuuuuu                                                    138


<210>  54
<211>  139
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR7 R2-R2 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  54
nnnnnnnnnn nnnnnnnnng uuugagagcu acccgggacg cggguccggg acaguagcaa       60

guucaaauaa ggcuaguccg uuaucaacuu cccgggacgc ggguccggga cagaaguggc      120

accgagucgg ugcuuuuuu                                                   139


<210>  55
<211>  137
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  MsgDiR8 R5-R5 Sequence


<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  n is a, c, g, or u

<400>  55
nnnnnnnnnn nnnnnnnnng uuugagagcu acugaggccu uggcgaggcu ucuuagcaag       60

uucaaauaag gcuaguccgu uaucaacuuc ugaggccuug gcgaggcuuc uaaguggcac      120

cgagucggug cuuuuuu                                                     137


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16-F primer

<400>  56
caacgcaccg aatagttacg                                                   20


<210>  57
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   p16-R primer

<400>  57
agcaccacca gcgtgtc                                                      17


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CEBPA-F primer

<400>  58
tataggctgg gcttcccctt                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   CEBPA-R primer

<400>  59
agctttctgg tgtgactcgg                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p14-F primer

<400>  60
gcaggttctt ggtgaccctc                                                   20


<210>  61
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p14-R primer

<400>  61
ccatcatcat gacctggtct tcta                                              24


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p15-F primer

<400>  62
tagtggagaa ggtgcgacag                                                   20


<210>  63
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p15-R primer

<400>  63
gcgctgccca tcatcatg                                                     18


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACTB-F primer

<400>  64
tgaagtgtga cgtggacatc                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ACTB-R primer

<400>  65
ggaggagcaa tgatcttgat                                                   20


<210>  66
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  GN2 Guide RNA

<400>  66
guuaggaaua aaagcuuuga                                                   20


<210>  67
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G2 Guide RNA

<400>  67
gcacucaaac acgccuuugc                                                   20


<210>  68
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G19 Guide RNA

<400>  68
gcucccccgc cugccagcaa                                                   20


<210>  69
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G36 Guide RNA

<400>  69
gcuaacugcc aaauugaauc g                                                 21


<210>  70
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G108 Guide RNA

<400>  70
guggccagcc agucagccga                                                   20


<210>  71
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G122 Guide RNA

<400>  71
gccgcagccg ccgagcgcac g                                                 21


<210>  72
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G110 Guide RNA

<400>  72
gacccucuac ccaccuggau                                                   20


<210>  73
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16-BSP-C-F1 primer

<400>  73
tggtttttgg attattgtgt aatttt                                            26


<210>  74
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16-BSP-C-R1 primer

<400>  74
ctttcctaat tataaaaacc ccacc                                             25


<210>  75
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   p16-BSP-4F primer

<400>  75
aatttggtag ttaggaaggt tgta                                              24


<210>  76
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16-BSP-4R primer

<400>  76
tccccaccta ccccccaca                                                    19


<210>  77
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16_BSP_original_F primer

<400>  77
tttttagagg atttgaggga tagg                                              24


<210>  78
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16_BSP_original_R primer

<400>  78
ctacctaatt ccaattcccc taca                                              24


<210>  79
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fp16-BSP-D3-F1 primer

<400>  79
tttaggtggg tagagggttt gtag                                              24


<210>  80
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rp16-BSP-D3-R1 primer

<400>  80
aactcctcat tcctcttcct taact                                             25


<210>  81
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   p16-E-BSP-F1 primer

<400>  81
ttaggtgggt agagggtttg tag                                               23


<210>  82
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   p16-E-BSP-R1 primer

<400>  82
caaactaaaa taaaataact ccatct                                            26


<210>  83
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16_BS_F primer

<400>  83
atttggtagt taggaaggtt gta                                               23


<210>  84
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   p16 _BS_R primer

<400>  84
ccaaaaaacc tccccttttt cc                                                22


<210>  85
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16_BS_F primer

<400>  85
atttggtagt taggaaggtt gta                                               23


<210>  86
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16_BS_R primer

<400>  86
ccaaaaaacc tccccttttt cc                                                22


<210>  87
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   p16 Exon 1 UF primer

<400>  87
ttattagagg gtggggtgga ttgt                                              24


<210>  88
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   p16 Exon 1 UR primer

<400>  88
ccacctaaat caacctccaa cca                                               23


<210>  89
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16 Exon 1 MF primer

<400>  89
ttattagagg gtggggcgga tcgc                                              24


<210>  90
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  p16 Exon 1 MR primer

<400>  90
ccacctaaat cgacctccga ccg                                               23


<210>  91
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16-F

<400>  91
ggtggggctc tcacaact                                                     18


<210>  92
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P16-R

<400>  92
ccttcctccg cgatacaa                                                     18


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P14-F (Positive Control)

<400>  93
agaagtctgc cgctcctcta                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P14-R (Positive Control)

<400>  94
acagatcaga cgtcaagccc                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P15-F (Positive Control)

<400>  95
gtgaagccca agtactgcct                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P15-F (Positive Control)

<400>  96
tcactgtgga gacgttggtg                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Down10K-1F (Negative Control)

<400>  97
aggagcccat agcttgtgga                                                   20


<210>  98
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Down10K-1R (Negative Control)

<400>  98
gatacttcca ctagacatct tgtca                                             25


<210>  99
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Up50K-1F (Negative Control)

<400>  99
ataaagcatt gcaggagctt aca                                               23


<210>  100
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Up50K-1R (Negative Control)

<400>  100
cctacacatt tttgtggcct gttt                                              24


<210>  101
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csp6I-NlaIII-1F primer

<400>  101
gcctccgacc gtaactattc g                                                 21


<210>  102
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csp6I-NlaIII-1R primer

<400>  102
aggacgaagt ttgcagggg                                                    19


<210>  103
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DpnII-NlaIII-1F primer

<400>  103
cattggaagg acggactcca tt                                                22


<210>  104
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DpnII-NlaIII-1R primer

<400>  104
tggaaagata ccgcggtcc                                                    19


<210>  105
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csp6I-NlaIII-C-502 primer

<400>  105
aatgatacgg cgaccaccga gatctacacc tctctattcg tcggcagcgt cagatgtgta       60

taagagacag aagccaagga agaggaatga gg                                     92


<210>  106
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csp6I-NlaIII-C-501 primer

<400>  106
aatgatacgg cgaccaccga gatctacact agatcgctcg tcggcagcgt cagatgtgta       60

taagagacag aagccaagga agaggaatga gg                                     92


<210>  107
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csp6I-NlaIII-N-703 primer

<400>  107
caagcagaag acggcatacg agatttctgc ctgtctcgtg ggctcggaga tgtgtataag       60

agacagccag ccagtcagcc gaag                                              84


<210>  108
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Csp6I-NlaIII-N-701 primer

<400>  108
caagcagaag acggcatacg agattcgcct tagtctcgtg ggctcggaga tgtgtataag       60

agacagccag ccagtcagcc gaag                                              84


<210>  109
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DpnII-NlaIII-D-501 primer

<400>  109
aatgatacgg cgaccaccga gatctacact agatcgctcg tcggcagcgt cagatgtgta       60

taagagacag tgctcagtgt tctagaagca ga                                     92


<210>  110
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DpnII-NlaIII-D-502 primer

<400>  110
aatgatacgg cgaccaccga gatctacacc tctctattcg tcggcagcgt cagatgtgta       60

taagagacag tgctcagtgt tctagaagca ga                                     92


<210>  111
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   DpnII-NlaIII-N-704 primer

<400>  111
caagcagaag acggcatacg agatgctcag gagtctcgtg ggctcggaga tgtgtataag       60

agacagggag agggggagag cagg                                              84


<210>  112
<211>  84
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DpnII-NlaIII-N-702 primer

<400>  112
caagcagaag acggcatacg agatctagta cggtctcgtg ggctcggaga tgtgtataag       60

agacagggag agggggagag cagg                                              84


<210>  113
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  G111 Guide RNA

<400>  113
gcccccaggg cgucgccagg                                                   20


