                         SEQUENCE LISTING



<110>  Duke University

       Wang, Qianben

       Cui, Zhifen

<110>  Ohio State Innovation Foundation

       Dong, Yizhou

 

<120>  NANOPARTICLE SYSTEMS FOR TARGETED DELIVERY OF CRISPR/CAS13 AND 

       METHODS OF USING SAME



<130>  20-1068-WO



<150>  US 62/873,295

<151>  2019-07-12



<160>  78    



<170>  PatentIn version 3.5



<210>  1

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Synthetic oligonucleotide



<400>  1

tcccggatag aaggcaaact cagtggggcg                                        30





<210>  2

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  2

agatctctta aggggtagcg ctgttcttca                                        30





<210>  3

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  3

agggggcata gttgacagca ggcatcagcg                                        30





<210>  4

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  4

caaaagggac ctggtgggtt ctgttctccc                                        30





<210>  5

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  5

atcgacattg ccggcacttg tgttcagttt                                        30





<210>  6

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  6

taatttggat gagaaatcac tttttctact                                        30





<210>  7

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  7

tggttttcat agtaaggtcc aatagctggt                                        30





<210>  8

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  8

cccctatcag ccaccagata ttgttcttcg                                        30





<210>  9

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  9

ctgaggcaat tccacaatgg tttctccggt                                        30





<210>  10

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  10

ttattgttat ctgattctgt gctttcaaat                                        30





<210>  11

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  11

acaccatgat ccatgtcttc actgctacag                                        30





<210>  12

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  12

cctgcatcaa tagcaacaga aatgggcccc                                        30





<210>  13

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  13

tctcagaatt cccgcaaatg ccgtccagta                                        30





<210>  14

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  14

tgagagcaat gtcgttattc ttggtcttag                                        30





<210>  15

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  15

cgtcacgttc ccgtatactc caggtctgag                                        30





<210>  16

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  16

cagactgata cccgtggttc tcatagcaag                                        30





<210>  17

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  17

cctcattacc gctacccatc aattcacgac                                        30





<210>  18

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  18

gccgtgctgc ccgttgctgt attccccgtt                                        30





<210>  19

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  19

ttgccttgag cgtgagaaca gtccacaagg                                        30





<210>  20

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  20

cacagcgaac tcggctctgt atttacaaga                                        30





<210>  21

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  21

tcctggtaca cgtcccgttt agtccgtcgc                                        30





<210>  22

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  22

gttttcaatc tctaggaccc actcgccaga                                        30





<210>  23

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  23

gcccagtcat taaacccatc tgcggagtag                                        30





<210>  24

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  24

cgaaaactaa agccagagcg cagctgcagg                                        30





<210>  25

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  25

cacctctagc cgtttgccga tgtccttggg                                        30





<210>  26

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  26

tgcctgctag gtcgggatga ttcttctcaa                                        30





<210>  27

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  27

cgtagccata cgaatggctc actttccggc                                        30





<210>  28

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  28

cagggcacat tgccgaactg tgtggcgctg                                        30





<210>  29

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  29

acagaaccca ccaggtccct tt                                                22





<210>  30

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  30

tacggaatgc gtttcttgcg gc                                                22





<210>  31

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  31

gtccccactg tctacgaggt                                                   20





<210>  32

<211>  19

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  32

cagacgacgg ggttggaag                                                    19





<210>  33

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  33

gagaaccgtt gtgttcgtct c                                                 21





<210>  34

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  34

gctctggtct ggtatccctt g                                                 21





<210>  35

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  35

gtcagtgtgg ttcttgttgg                                                   20





<210>  36

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  36

aaggactcat gacctgcatc                                                   20





<210>  37

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  37

atggcacgaa tgaggaagag                                                   20





<210>  38

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  38

gaaaaagcct ccccttcttg                                                   20





<210>  39

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  39

cctggttgct atgggtggta g                                                 21





<210>  40

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  40

aagtggtaat agtccccgaa ga                                                22





<210>  41

<211>  23

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  41

tcggtgacta ttaccacttc tgg                                               23





<210>  42

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  42

ctcctgatac acgtccctct t                                                 21





<210>  43

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  43

cctgaaccct aaggccaacc                                                   20





<210>  44

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  44

atggcgtgag ggagagcata                                                   20





<210>  45

<211>  17

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  45

aggcaccagg gcgtgat                                                      17





<210>  46

<211>  23

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  46

gcccacatag gaatccttct gac                                               23





<210>  47

<211>  21

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  47

ttgcgagatg aagtggaaag g                                                 21





<210>  48

<211>  23

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  48

caagaagtga aaggtgactg gtt                                               23





<210>  49

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  49

aacagagagg atttcgtttc cg                                                22





<210>  50

<211>  23

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  50

tttgacctga gggtaagact tct                                               23





<210>  51

<211>  20

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  51

gggatcagct ccgtggatct                                                   20





<210>  52

<211>  23

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  52

tgcactttgg tactcttgaa gtt                                               23





<210>  53

<211>  23

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  53

atgactgagt acaaactggt ggt                                               23





<210>  54

<211>  23

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  54

catgtattgg tctctcatgg cac                                               23





<210>  55

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  55

tcccggatag aaggcaaact ca                                                22





<210>  56

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  56

agatctctta aggggtagcg ct                                                22





<210>  57

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  57

agggggcata gttgacagca gg                                                22





<210>  58

<211>  22

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  58

caaaagggac ctggtgggtt ct                                                22





<210>  59

<211>  11

<212>  PRT

<213>  Artificial Sequence



<220>

<223>  synthetic cell-penetrating TAT peptide



<400>  59



Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 

1               5                   10      





<210>  60

<211>  30

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  60

aacccctacc aactggtcgg ggtttgaaac                                        30





<210>  61

<211>  36

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide



<400>  61

caagtaaacc cctaccaact ggtcggggtt tgaaac                                 36





<210>  62

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (46)..(48)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (51)..(51)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (55)..(58)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  62

caaguaaacc ccuaccaacu ggucgggguu ugaaaccaaa agggaccugg uggguucugu       60



ucuccccaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  63

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (37)..(37)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (40)..(42)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (46)..(46)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (53)..(54)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (56)..(59)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  63

caaguaaacc ccuaccaacu ggucgggguu ugaaacuaau uuggaugaga aaucacuuuu       60



ucuacucaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  64

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (37)..(39)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (41)..(41)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (43)..(44)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (47)..(47)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (50)..(50)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (56)..(56)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  64

caaguaaacc ccuaccaacu ggucgggguu ugaaacccug caucaauagc aacagaaaug       60



ggcccccaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  65

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (37)..(37)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (41)..(42)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (45)..(45)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (47)..(49)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (51)..(51)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (54)..(58)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  65

caaguaaacc ccuaccaacu ggucgggguu ugaaaccaga cugauacccg ugguucucau       60



agcaagcaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  66

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (37)..(38)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (40)..(43)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (47)..(47)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (49)..(49)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (55)..(55)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (58)..(59)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  66

caaguaaacc ccuaccaacu ggucgggguu ugaaacuugc cuugagcgug agaacagucc       60



acaaggcaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  67

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (37)..(38)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (40)..(41)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (47)..(47)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (49)..(49)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (51)..(52)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (54)..(54)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (56)..(58)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  67

caaguaaacc ccuaccaacu ggucgggguu ugaaacucac cagaagcgua ccauacucac       60



gaacagcaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  68

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (38)..(40)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (43)..(44)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (46)..(47)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (51)..(53)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (55)..(57)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (59)..(59)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  68

caaguaaacc ccuaccaacu ggucgggguu ugaaacgccc agucauuaaa cccaucugcg       60



gaguagcaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  69

<211>  102

<212>  RNA

<213>  Artificial Sequence



<220>

<223>  synthetic oligonucleotide





<220>

<221>  misc_feature

<222>  (1)..(3)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (1)..(2)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (2)..(3)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (3)..(4)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(8)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (7)..(9)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (8)..(9)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (9)..(10)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (37)..(37)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (42)..(42)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (44)..(44)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (46)..(47)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (49)..(50)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (54)..(55)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (57)..(57)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (59)..(59)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (99)..(100)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (99)..(101)

<223>  2'-OMe



<220>

<221>  misc_feature

<222>  (100)..(101)

<223>  phosphorothioate linkage



<220>

<221>  misc_feature

<222>  (101)..(102)

<223>  phosphorothioate linkage



<400>  69

caaguaaacc ccuaccaacu ggucgggguu ugaaaccagg gcacauugcc gaacugugug       60



gcgcugcaag uaaaccccua ccaacugguc gggguuugaa ac                         102





<210>  70

<211>  3786

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  NLS-CasRx-NLS-HA-T2A-EGFP portion of Addgene #109049



<400>  70

atgagcccca agaagaagag aaaggtggag gccagcatcg aaaaaaaaaa gtccttcgcc       60



aagggcatgg gcgtgaagtc cacactcgtg tccggctcca aagtgtacat gacaaccttc      120



gccgaaggca gcgacgccag gctggaaaag atcgtggagg gcgacagcat caggagcgtg      180



aatgagggcg aggccttcag cgctgaaatg gccgataaaa acgccggcta taagatcggc      240



aacgccaaat tcagccatcc taagggctac gccgtggtgg ctaacaaccc tctgtataca      300



ggacccgtcc agcaggatat gctcggcctg aaggaaactc tggaaaagag gtacttcggc      360



gagagcgctg atggcaatga caatatttgt atccaggtga tccataacat cctggacatt      420



gaaaaaatcc tcgccgaata cattaccaac gccgcctacg ccgtcaacaa tatctccggc      480



ctggataagg acattattgg attcggcaag ttctccacag tgtataccta cgacgaattc      540



aaagaccccg agcaccatag ggccgctttc aacaataacg ataagctcat caacgccatc      600



aaggcccagt atgacgagtt cgacaacttc ctcgataacc ccagactcgg ctatttcggc      660



caggcctttt tcagcaagga gggcagaaat tacatcatca attacggcaa cgaatgctat      720



gacattctgg ccctcctgag cggactgagg cactgggtgg tccataacaa cgaagaagag      780



tccaggatct ccaggacctg gctctacaac ctcgataaga acctcgacaa cgaatacatc      840



tccaccctca actacctcta cgacaggatc accaatgagc tgaccaactc cttctccaag      900



aactccgccg ccaacgtgaa ctatattgcc gaaactctgg gaatcaaccc tgccgaattc      960



gccgaacaat atttcagatt cagcattatg aaagagcaga aaaacctcgg attcaatatc     1020



accaagctca gggaagtgat gctggacagg aaggatatgt ccgagatcag gaaaaatcat     1080



aaggtgttcg actccatcag gaccaaggtc tacaccatga tggactttgt gatttatagg     1140



tattacatcg aagaggatgc caaggtggct gccgccaata agtccctccc cgataatgag     1200



aagtccctga gcgagaagga tatctttgtg attaacctga ggggctcctt caacgacgac     1260



cagaaggatg ccctctacta cgatgaagct aatagaattt ggagaaagct cgaaaatatc     1320



atgcacaaca tcaaggaatt taggggaaac aagacaagag agtataagaa gaaggacgcc     1380



cctagactgc ccagaatcct gcccgctggc cgtgatgttt ccgccttcag caaactcatg     1440



tatgccctga ccatgttcct ggatggcaag gagatcaacg acctcctgac caccctgatt     1500



aataaattcg ataacatcca gagcttcctg aaggtgatgc ctctcatcgg agtcaacgct     1560



aagttcgtgg aggaatacgc ctttttcaaa gactccgcca agatcgccga tgagctgagg     1620



ctgatcaagt ccttcgctag aatgggagaa cctattgccg atgccaggag ggccatgtat     1680



atcgacgcca tccgtatttt aggaaccaac ctgtcctatg atgagctcaa ggccctcgcc     1740



gacacctttt ccctggacga gaacggaaac aagctcaaga aaggcaagca cggcatgaga     1800



aatttcatta ttaataacgt gatcagcaat aaaaggttcc actacctgat cagatacggt     1860



gatcctgccc acctccatga gatcgccaaa aacgaggccg tggtgaagtt cgtgctcggc     1920



aggatcgctg acatccagaa aaaacagggc cagaacggca agaaccagat cgacaggtac     1980



tacgaaactt gtatcggaaa ggataagggc aagagcgtga gcgaaaaggt ggacgctctc     2040



acaaagatca tcaccggaat gaactacgac caattcgaca agaaaaggag cgtcattgag     2100



gacaccggca gggaaaacgc cgagagggag aagtttaaaa agatcatcag cctgtacctc     2160



accgtgatct accacatcct caagaatatt gtcaatatca acgccaggta cgtcatcgga     2220



ttccattgcg tcgagcgtga tgctcaactg tacaaggaga aaggctacga catcaatctc     2280



aagaaactgg aagagaaggg attcagctcc gtcaccaagc tctgcgctgg cattgatgaa     2340



actgcccccg ataagagaaa ggacgtggaa aaggagatgg ctgaaagagc caaggagagc     2400



attgacagcc tcgagagcgc caaccccaag ctgtatgcca attacatcaa atacagcgac     2460



gagaagaaag ccgaggagtt caccaggcag attaacaggg agaaggccaa aaccgccctg     2520



aacgcctacc tgaggaacac caagtggaat gtgatcatca gggaggacct cctgagaatt     2580



gacaacaaga catgtaccct gttcagaaac aaggccgtcc acctggaagt ggccaggtat     2640



gtccacgcct atatcaacga cattgccgag gtcaattcct acttccaact gtaccattac     2700



atcatgcaga gaattatcat gaatgagagg tacgagaaaa gcagcggaaa ggtgtccgag     2760



tacttcgacg ctgtgaatga cgagaagaag tacaacgata ggctcctgaa actgctgtgt     2820



gtgcctttcg gctactgtat ccccaggttt aagaacctga gcatcgaggc cctgttcgat     2880



aggaacgagg ccgccaagtt cgacaaggag aaaaagaagg tgtccggcaa ttccggatcc     2940



ggacctaaga aaaagaggaa ggtggcggcc gcttacccat acgatgttcc agattacgct     3000



gctagcggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat     3060



cctggcccag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag     3120



ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc     3180



acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg     3240



cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac     3300



atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc     3360



atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac     3420



accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg     3480



gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag     3540



aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag     3600



ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac     3660



aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac     3720



atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac     3780



aagtaa                                                                3786





<210>  71

<211>  24

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  NLS sequence from NLS-CasRx-NLS-HA-T2A-EGFP (Addgene #109049)



<400>  71

agccccaaga agaagagaaa ggtg                                              24





<210>  72

<211>  2916

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  CasRx sequence from NLS-CasRx-NLS-HA-T2A-EGFP (Addgene #109049)



<400>  72

gaggccagca tcgaaaaaaa aaagtccttc gccaagggca tgggcgtgaa gtccacactc       60



gtgtccggct ccaaagtgta catgacaacc ttcgccgaag gcagcgacgc caggctggaa      120



aagatcgtgg agggcgacag catcaggagc gtgaatgagg gcgaggcctt cagcgctgaa      180



atggccgata aaaacgccgg ctataagatc ggcaacgcca aattcagcca tcctaagggc      240



tacgccgtgg tggctaacaa ccctctgtat acaggacccg tccagcagga tatgctcggc      300



ctgaaggaaa ctctggaaaa gaggtacttc ggcgagagcg ctgatggcaa tgacaatatt      360



tgtatccagg tgatccataa catcctggac attgaaaaaa tcctcgccga atacattacc      420



aacgccgcct acgccgtcaa caatatctcc ggcctggata aggacattat tggattcggc      480



aagttctcca cagtgtatac ctacgacgaa ttcaaagacc ccgagcacca tagggccgct      540



ttcaacaata acgataagct catcaacgcc atcaaggccc agtatgacga gttcgacaac      600



ttcctcgata accccagact cggctatttc ggccaggcct ttttcagcaa ggagggcaga      660



aattacatca tcaattacgg caacgaatgc tatgacattc tggccctcct gagcggactg      720



aggcactggg tggtccataa caacgaagaa gagtccagga tctccaggac ctggctctac      780



aacctcgata agaacctcga caacgaatac atctccaccc tcaactacct ctacgacagg      840



atcaccaatg agctgaccaa ctccttctcc aagaactccg ccgccaacgt gaactatatt      900



gccgaaactc tgggaatcaa ccctgccgaa ttcgccgaac aatatttcag attcagcatt      960



atgaaagagc agaaaaacct cggattcaat atcaccaagc tcagggaagt gatgctggac     1020



aggaaggata tgtccgagat caggaaaaat cataaggtgt tcgactccat caggaccaag     1080



gtctacacca tgatggactt tgtgatttat aggtattaca tcgaagagga tgccaaggtg     1140



gctgccgcca ataagtccct ccccgataat gagaagtccc tgagcgagaa ggatatcttt     1200



gtgattaacc tgaggggctc cttcaacgac gaccagaagg atgccctcta ctacgatgaa     1260



gctaatagaa tttggagaaa gctcgaaaat atcatgcaca acatcaagga atttagggga     1320



aacaagacaa gagagtataa gaagaaggac gcccctagac tgcccagaat cctgcccgct     1380



ggccgtgatg tttccgcctt cagcaaactc atgtatgccc tgaccatgtt cctggatggc     1440



aaggagatca acgacctcct gaccaccctg attaataaat tcgataacat ccagagcttc     1500



ctgaaggtga tgcctctcat cggagtcaac gctaagttcg tggaggaata cgcctttttc     1560



aaagactccg ccaagatcgc cgatgagctg aggctgatca agtccttcgc tagaatggga     1620



gaacctattg ccgatgccag gagggccatg tatatcgacg ccatccgtat tttaggaacc     1680



aacctgtcct atgatgagct caaggccctc gccgacacct tttccctgga cgagaacgga     1740



aacaagctca agaaaggcaa gcacggcatg agaaatttca ttattaataa cgtgatcagc     1800



aataaaaggt tccactacct gatcagatac ggtgatcctg cccacctcca tgagatcgcc     1860



aaaaacgagg ccgtggtgaa gttcgtgctc ggcaggatcg ctgacatcca gaaaaaacag     1920



ggccagaacg gcaagaacca gatcgacagg tactacgaaa cttgtatcgg aaaggataag     1980



ggcaagagcg tgagcgaaaa ggtggacgct ctcacaaaga tcatcaccgg aatgaactac     2040



gaccaattcg acaagaaaag gagcgtcatt gaggacaccg gcagggaaaa cgccgagagg     2100



gagaagttta aaaagatcat cagcctgtac ctcaccgtga tctaccacat cctcaagaat     2160



attgtcaata tcaacgccag gtacgtcatc ggattccatt gcgtcgagcg tgatgctcaa     2220



ctgtacaagg agaaaggcta cgacatcaat ctcaagaaac tggaagagaa gggattcagc     2280



tccgtcacca agctctgcgc tggcattgat gaaactgccc ccgataagag aaaggacgtg     2340



gaaaaggaga tggctgaaag agccaaggag agcattgaca gcctcgagag cgccaacccc     2400



aagctgtatg ccaattacat caaatacagc gacgagaaga aagccgagga gttcaccagg     2460



cagattaaca gggagaaggc caaaaccgcc ctgaacgcct acctgaggaa caccaagtgg     2520



aatgtgatca tcagggagga cctcctgaga attgacaaca agacatgtac cctgttcaga     2580



aacaaggccg tccacctgga agtggccagg tatgtccacg cctatatcaa cgacattgcc     2640



gaggtcaatt cctacttcca actgtaccat tacatcatgc agagaattat catgaatgag     2700



aggtacgaga aaagcagcgg aaaggtgtcc gagtacttcg acgctgtgaa tgacgagaag     2760



aagtacaacg ataggctcct gaaactgctg tgtgtgcctt tcggctactg tatccccagg     2820



tttaagaacc tgagcatcga ggccctgttc gataggaacg aggccgccaa gttcgacaag     2880



gagaaaaaga aggtgtccgg caattccgga tccgga                               2916





<210>  73

<211>  36

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  HA sequence from NLS-CasRx-NLS-HA-T2A-EGFP (Addgene #109049)



<400>  73

gcggccgctt acccatacga tgttccagat tacgct                                 36





<210>  74

<211>  54

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  T2A sequence from NLS-CasRx-NLS-HA-T2A-EGFP (Addgene #109049)



<400>  74

gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg ccca             54





<210>  75

<211>  714

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  EGFP sequence from NLS-CasRx-NLS-HA-T2A-EGFP (Addgene #109049)



<400>  75

gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc       60



gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc      120



aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc      180



gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag      240



cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc      300



aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg      360



aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag      420



ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc      480



atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac      540



cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac      600



ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg      660



ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caag            714





<210>  76

<211>  2903

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  CasRx gRNA cloning backbone (Addgene #109053)



<400>  76

gctagcgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt       60



agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga      120



cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac      180



tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg      240



aaaggacgaa acaccgaacc cctaccaact ggtcggggtt tgaaacgggt cttcgagaag      300



accttttttt tgaattctga tgcggtattt tctccttacg catctgtgcg gtatttcaca      360



ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt      420



gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc      480



gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg      540



gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat      600



ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg      660



ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct      720



atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa      780



aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt      840



ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac      900



ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga      960



caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa     1020



cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata     1080



atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt     1140



ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg     1200



cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt     1260



cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta     1320



aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc     1380



ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa     1440



gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc     1500



cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt     1560



acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact     1620



gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac     1680



aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata     1740



ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta     1800



ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg     1860



gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat     1920



aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt     1980



aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga     2040



aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa     2100



gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag     2160



gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac     2220



tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     2280



gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     2340



caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     2400



actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     2460



acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     2520



cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     2580



gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     2640



cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     2700



gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     2760



tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     2820



tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     2880



gccttttgct ggccttttgc tca                                             2903





<210>  77

<211>  2945

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  CasRx pre-gRNA cloning backbone (Addgene #109054)



<400>  77

gctagcgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt       60



agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga      120



cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac      180



tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg      240



aaaggacgaa acaccgcaag taaaccccta ccaactggtc ggggtttgaa acgggtcttc      300



gagaagacct caagtaaacc cctaccaact ggtcggggtt tgaaactttt tttgaattct      360



gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa      420



ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc      480



gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt      540



ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc      600



cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt      660



agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt      720



aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt      780



gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa      840



aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt      900



acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac      960



gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc     1020



gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc     1080



ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca     1140



ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat     1200



tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa     1260



aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt     1320



tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag     1380



ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt     1440



tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg     1500



gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag     1560



aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta     1620



agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg     1680



acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta     1740



actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac     1800



accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt     1860



actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca     1920



cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag     1980



cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta     2040



gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag     2100



ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt     2160



tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat     2220



aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     2280



gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     2340



acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     2400



tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag     2460



ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     2520



atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     2580



agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     2640



cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     2700



agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     2760



acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     2820



gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     2880



ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt     2940



gctca                                                                 2945





<210>  78

<211>  12990

<212>  DNA

<213>  Artificial Sequence



<220>

<223>  Full length sequence for Addgene #109049 containing, in part, 

       NLS-CasRx-NLS-HA-T2A-EGFP



<400>  78

gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg       60



atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt      120



gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc      180



tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac      240



attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat      300



atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg      360



acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt      420



tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag      480



tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc      540



attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag      600



tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt      660



ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc      720



accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg      780



gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct      840



ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt      900



aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac      960



tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc     1020



gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc     1080



ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa     1140



ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg     1200



ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata     1260



aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc     1320



tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga     1380



caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc     1440



aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca     1500



aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg     1560



agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga     1620



gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata     1680



ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg     1740



acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg     1800



ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag     1860



ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt     1920



tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt     1980



aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt     2040



aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag     2100



aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata     2160



acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta     2220



agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta     2280



tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa     2340



gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt     2400



gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat     2460



tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa     2520



agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag     2580



agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt     2640



gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt     2700



gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa     2760



ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc     2820



gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc     2880



tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc     2940



ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta     3000



cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc     3060



ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg     3120



cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat     3180



ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc     3240



gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg     3300



tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg     3360



acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat     3420



cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg     3480



gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg     3540



ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg     3600



tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag     3660



tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg     3720



ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct     3780



ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct     3840



tccatttcag gtgtcgtgac gtacggccac catgagcccc aagaagaaga gaaaggtgga     3900



ggccagcatc gaaaaaaaaa agtccttcgc caagggcatg ggcgtgaagt ccacactcgt     3960



gtccggctcc aaagtgtaca tgacaacctt cgccgaaggc agcgacgcca ggctggaaaa     4020



gatcgtggag ggcgacagca tcaggagcgt gaatgagggc gaggccttca gcgctgaaat     4080



ggccgataaa aacgccggct ataagatcgg caacgccaaa ttcagccatc ctaagggcta     4140



cgccgtggtg gctaacaacc ctctgtatac aggacccgtc cagcaggata tgctcggcct     4200



gaaggaaact ctggaaaaga ggtacttcgg cgagagcgct gatggcaatg acaatatttg     4260



tatccaggtg atccataaca tcctggacat tgaaaaaatc ctcgccgaat acattaccaa     4320



cgccgcctac gccgtcaaca atatctccgg cctggataag gacattattg gattcggcaa     4380



gttctccaca gtgtatacct acgacgaatt caaagacccc gagcaccata gggccgcttt     4440



caacaataac gataagctca tcaacgccat caaggcccag tatgacgagt tcgacaactt     4500



cctcgataac cccagactcg gctatttcgg ccaggccttt ttcagcaagg agggcagaaa     4560



ttacatcatc aattacggca acgaatgcta tgacattctg gccctcctga gcggactgag     4620



gcactgggtg gtccataaca acgaagaaga gtccaggatc tccaggacct ggctctacaa     4680



cctcgataag aacctcgaca acgaatacat ctccaccctc aactacctct acgacaggat     4740



caccaatgag ctgaccaact ccttctccaa gaactccgcc gccaacgtga actatattgc     4800



cgaaactctg ggaatcaacc ctgccgaatt cgccgaacaa tatttcagat tcagcattat     4860



gaaagagcag aaaaacctcg gattcaatat caccaagctc agggaagtga tgctggacag     4920



gaaggatatg tccgagatca ggaaaaatca taaggtgttc gactccatca ggaccaaggt     4980



ctacaccatg atggactttg tgatttatag gtattacatc gaagaggatg ccaaggtggc     5040



tgccgccaat aagtccctcc ccgataatga gaagtccctg agcgagaagg atatctttgt     5100



gattaacctg aggggctcct tcaacgacga ccagaaggat gccctctact acgatgaagc     5160



taatagaatt tggagaaagc tcgaaaatat catgcacaac atcaaggaat ttaggggaaa     5220



caagacaaga gagtataaga agaaggacgc ccctagactg cccagaatcc tgcccgctgg     5280



ccgtgatgtt tccgccttca gcaaactcat gtatgccctg accatgttcc tggatggcaa     5340



ggagatcaac gacctcctga ccaccctgat taataaattc gataacatcc agagcttcct     5400



gaaggtgatg cctctcatcg gagtcaacgc taagttcgtg gaggaatacg cctttttcaa     5460



agactccgcc aagatcgccg atgagctgag gctgatcaag tccttcgcta gaatgggaga     5520



acctattgcc gatgccagga gggccatgta tatcgacgcc atccgtattt taggaaccaa     5580



cctgtcctat gatgagctca aggccctcgc cgacaccttt tccctggacg agaacggaaa     5640



caagctcaag aaaggcaagc acggcatgag aaatttcatt attaataacg tgatcagcaa     5700



taaaaggttc cactacctga tcagatacgg tgatcctgcc cacctccatg agatcgccaa     5760



aaacgaggcc gtggtgaagt tcgtgctcgg caggatcgct gacatccaga aaaaacaggg     5820



ccagaacggc aagaaccaga tcgacaggta ctacgaaact tgtatcggaa aggataaggg     5880



caagagcgtg agcgaaaagg tggacgctct cacaaagatc atcaccggaa tgaactacga     5940



ccaattcgac aagaaaagga gcgtcattga ggacaccggc agggaaaacg ccgagaggga     6000



gaagtttaaa aagatcatca gcctgtacct caccgtgatc taccacatcc tcaagaatat     6060



tgtcaatatc aacgccaggt acgtcatcgg attccattgc gtcgagcgtg atgctcaact     6120



gtacaaggag aaaggctacg acatcaatct caagaaactg gaagagaagg gattcagctc     6180



cgtcaccaag ctctgcgctg gcattgatga aactgccccc gataagagaa aggacgtgga     6240



aaaggagatg gctgaaagag ccaaggagag cattgacagc ctcgagagcg ccaaccccaa     6300



gctgtatgcc aattacatca aatacagcga cgagaagaaa gccgaggagt tcaccaggca     6360



gattaacagg gagaaggcca aaaccgccct gaacgcctac ctgaggaaca ccaagtggaa     6420



tgtgatcatc agggaggacc tcctgagaat tgacaacaag acatgtaccc tgttcagaaa     6480



caaggccgtc cacctggaag tggccaggta tgtccacgcc tatatcaacg acattgccga     6540



ggtcaattcc tacttccaac tgtaccatta catcatgcag agaattatca tgaatgagag     6600



gtacgagaaa agcagcggaa aggtgtccga gtacttcgac gctgtgaatg acgagaagaa     6660



gtacaacgat aggctcctga aactgctgtg tgtgcctttc ggctactgta tccccaggtt     6720



taagaacctg agcatcgagg ccctgttcga taggaacgag gccgccaagt tcgacaagga     6780



gaaaaagaag gtgtccggca attccggatc cggacctaag aaaaagagga aggtggcggc     6840



cgcttaccca tacgatgttc cagattacgc tgctagcggc agtggagagg gcagaggaag     6900



tctgctaaca tgcggtgacg tcgaggagaa tcctggccca gtgagcaagg gcgaggagct     6960



gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt     7020



cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat     7080



ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg     7140



cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc     7200



catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa     7260



gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg     7320



catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag     7380



ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat     7440



ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc     7500



catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct     7560



gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc     7620



cgggatcact ctcggcatgg acgagctgta caagtaagaa ttcgatatca agcttatcga     7680



taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc     7740



tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg     7800



tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt     7860



gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac     7920



tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc     7980



tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct     8040



gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct     8100



cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct     8160



caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct     8220



tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg     8280



tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa     8340



tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc     8400



tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa     8460



agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct     8520



gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg     8580



gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa     8640



ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg     8700



gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca     8760



tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg     8820



agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc     8880



ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct     8940



cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag     9000



cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct     9060



tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc     9120



attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg     9180



aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg     9240



cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa     9300



gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc     9360



ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag     9420



ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca     9480



aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc     9540



gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa     9600



cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct     9660



attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt     9720



gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat     9780



gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag     9840



tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat     9900



cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt     9960



tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg    10020



cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg    10080



atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac    10140



gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg    10200



cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc    10260



gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc    10320



caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag    10380



ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc    10440



atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc    10500



aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc    10560



accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg    10620



atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca    10680



gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt    10740



tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata    10800



ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat    10860



tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg    10920



ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag    10980



tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt    11040



ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg    11100



ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg    11160



gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag    11220



gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga    11280



cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct    11340



ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc    11400



tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg    11460



gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc    11520



tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca    11580



ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag    11640



ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct    11700



ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc    11760



accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga    11820



tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca    11880



cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat    11940



taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac    12000



caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt    12060



gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt    12120



gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag    12180



ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct    12240



attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt    12300



gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc    12360



tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt    12420



agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg    12480



gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg    12540



actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct    12600



tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc    12660



attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt    12720



tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt    12780



tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg    12840



aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat    12900



tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg    12960



cgcacatttc cccgaaaagt gccacctgac                                     12990





