                         SEQUENCE LISTING

<110>  Shenzhen Hua Yao Kang Ming Biopharmaceutial Co.,Ltd.
 
<120>  ENGINEERED VACCINIA VIRUS

<130>  SZ190534-US

<150>  US62/873,221
<151>  2019-07-12

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  100
<212>  PRT
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(100)
<223>  mB5R amino acid sequence

<400>  1

Met Lys Thr Ile Ser Val Val Thr Leu Leu Cys Val Leu Pro Ala Val 
1               5                   10                  15      


Val Tyr Ser Cys Val Arg Thr Asn Glu Lys Phe Asp Pro Val Asp Asp 
            20                  25                  30          


Gly Pro Asp Asp Glu Thr Asp Leu Ser Lys Leu Ser Lys Asp Val Val 
        35                  40                  45              


Gln Tyr Glu Gln Glu Ile Glu Ser Leu Glu Ala Thr Tyr His Ile Ile 
    50                  55                  60                  


Ile Val Ala Leu Thr Ile Met Gly Val Ile Phe Leu Ile Ser Val Ile 
65                  70                  75                  80  


Val Leu Val Cys Ser Cys Asp Lys Asn Asn Asp Gln Tyr Lys Phe His 
                85                  90                  95      


Lys Leu Leu Pro 
            100 


<210>  2
<211>  303
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(303)
<223>  mB5R nucleotide sequence

<400>  2
atgaaaacga tttccgttgt tacgttgtta tgcgtactac ctgctgttgt ttattcatgt       60

gtacgaacta acgaaaaatt tgatccagtg gatgatggtc ccgacgatga gacagatttg      120

agcaaactct cgaaagacgt tgtacaatat gaacaagaaa tagaatcgtt agaagcaact      180

tatcatataa tcatagtggc gttgacaatt atgggcgtca tatttttaat ctccgttata      240

gtattagttt gttcctgtga caaaaataat gaccaatata agttccataa attgctaccg      300

tga                                                                    303


<210>  3
<211>  153
<212>  PRT
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(153)
<223>  sB5R amino acid sequence

<400>  3

Met Lys Thr Ile Ser Val Val Thr Leu Leu Cys Val Leu Pro Ala Val 
1               5                   10                  15      


Val Tyr Ser Thr Cys Thr Val Pro Thr Met Asn Asn Ala Lys Leu Thr 
            20                  25                  30          


Ser Thr Glu Thr Ser Phe Asn Asp Lys Gln Lys Val Thr Phe Thr Cys 
        35                  40                  45              


Asp Gln Gly Tyr His Ser Ser Asp Pro Asn Ala Val Cys Glu Thr Asp 
    50                  55                  60                  


Lys Trp Lys Tyr Glu Asn Pro Cys Cys Val Arg Thr Asn Glu Lys Phe 
65                  70                  75                  80  


Asp Pro Val Asp Asp Gly Pro Asp Asp Glu Thr Asp Leu Ser Lys Leu 
                85                  90                  95      


Ser Lys Asp Val Val Gln Tyr Glu Gln Glu Ile Glu Ser Leu Glu Ala 
            100                 105                 110         


Thr Tyr His Ile Ile Ile Val Ala Leu Thr Ile Met Gly Val Ile Phe 
        115                 120                 125             


Leu Ile Ser Val Ile Val Leu Val Cys Ser Cys Asp Lys Asn Asn Asp 
    130                 135                 140                 


Gln Tyr Lys Phe His Lys Leu Leu Pro 
145                 150             


<210>  4
<211>  462
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(462)
<223>  sB5R nucleotide sequence

<400>  4
atgaaaacga tttccgttgt tacgttgtta tgcgtactac ctgctgttgt ttattcaaca       60

tgtactgtac ccactatgaa taacgctaaa ttaacgtcta ccgaaacatc gtttaatgat      120

aaacagaaag ttacatttac atgtgatcag ggatatcatt cttcggatcc aaatgctgtc      180

tgcgaaacag ataaatggaa atacgaaaat ccatgctgtg tacgaactaa cgaaaaattt      240

gatccagtgg atgatggtcc cgacgatgag acagatttga gcaaactctc gaaagacgtt      300

gtacaatatg aacaagaaat agaatcgtta gaagcaactt atcatataat catagtggcg      360

ttgacaatta tgggcgtcat atttttaatc tccgttatag tattagtttg ttcctgtgac      420

aaaaataatg accaatataa gttccataaa ttgctaccgt ga                         462


<210>  5
<211>  462
<212>  PRT
<213>  Mus musculus


<220>
<221>  Artificial sequence
<222>  (1)..(462)
<223>  SPD-m4-1BBL amino acid sequence

<400>  5

Met Leu Pro Phe Leu Ser Met Leu Val Leu Leu Val Gln Pro Leu Gly 
1               5                   10                  15      


Asn Leu Gly Ala Glu Met Lys Ser Leu Ser Gln Arg Ser Val Pro Asn 
            20                  25                  30          


Thr Cys Thr Leu Val Met Cys Ser Pro Thr Glu Asn Gly Leu Pro Gly 
        35                  40                  45              


Arg Asp Gly Arg Asp Gly Arg Glu Gly Pro Arg Gly Glu Lys Gly Asp 
    50                  55                  60                  


Pro Gly Leu Pro Gly Pro Met Gly Leu Ser Gly Leu Gln Gly Pro Thr 
65                  70                  75                  80  


Gly Pro Val Gly Pro Lys Gly Glu Asn Gly Ser Ala Gly Glu Pro Gly 
                85                  90                  95      


Pro Lys Gly Glu Arg Gly Leu Ser Gly Pro Pro Gly Leu Pro Gly Ile 
            100                 105                 110         


Pro Gly Pro Ala Gly Lys Glu Gly Pro Ser Gly Lys Gln Gly Asn Ile 
        115                 120                 125             


Gly Pro Gln Gly Lys Pro Gly Pro Lys Gly Glu Ala Gly Pro Lys Gly 
    130                 135                 140                 


Glu Val Gly Ala Pro Gly Met Gln Gly Ser Thr Gly Ala Lys Gly Ser 
145                 150                 155                 160 


Thr Gly Pro Lys Gly Glu Arg Gly Ala Pro Gly Val Gln Gly Ala Pro 
                165                 170                 175     


Gly Asn Ala Gly Ala Ala Gly Pro Ala Gly Pro Ala Gly Pro Gln Gly 
            180                 185                 190         


Ala Pro Gly Ser Arg Gly Pro Pro Gly Leu Lys Gly Asp Arg Gly Val 
        195                 200                 205             


Pro Gly Asp Arg Gly Ile Lys Gly Glu Ser Gly Leu Pro Asp Ser Ala 
    210                 215                 220                 


Ala Leu Arg Gln Gln Met Glu Ala Leu Lys Gly Lys Leu Gln Arg Leu 
225                 230                 235                 240 


Glu Val Ala Phe Ser His Tyr Gln Lys Ala Ala Leu Phe Pro Asp Gly 
                245                 250                 255     


Arg Thr Glu Pro Arg Pro Ala Leu Thr Ile Thr Thr Ser Pro Asn Leu 
            260                 265                 270         


Gly Thr Arg Glu Asn Asn Ala Asp Gln Val Thr Pro Val Ser His Ile 
        275                 280                 285             


Gly Cys Pro Asn Thr Thr Gln Gln Gly Ser Pro Val Phe Ala Lys Leu 
    290                 295                 300                 


Leu Ala Lys Asn Gln Ala Ser Leu Cys Asn Thr Thr Leu Asn Trp His 
305                 310                 315                 320 


Ser Gln Asp Gly Ala Gly Ser Ser Tyr Leu Ser Gln Gly Leu Arg Tyr 
                325                 330                 335     


Glu Glu Asp Lys Lys Glu Leu Val Val Asp Ser Pro Gly Leu Tyr Tyr 
            340                 345                 350         


Val Phe Leu Glu Leu Lys Leu Ser Pro Thr Phe Thr Asn Thr Gly His 
        355                 360                 365             


Lys Val Gln Gly Trp Val Ser Leu Val Leu Gln Ala Lys Pro Gln Val 
    370                 375                 380                 


Asp Asp Phe Asp Asn Leu Ala Leu Thr Val Glu Leu Phe Pro Cys Ser 
385                 390                 395                 400 


Met Glu Asn Lys Leu Val Asp Arg Ser Trp Ser Gln Leu Leu Leu Leu 
                405                 410                 415     


Lys Ala Gly His Arg Leu Ser Val Gly Leu Arg Ala Tyr Leu His Gly 
            420                 425                 430         


Ala Gln Asp Ala Tyr Arg Asp Trp Glu Leu Ser Tyr Pro Asn Thr Thr 
        435                 440                 445             


Ser Phe Gly Leu Phe Leu Val Lys Pro Asp Asn Pro Trp Glu 
    450                 455                 460         


<210>  6
<211>  1389
<212>  DNA
<213>  Mus musculus


<220>
<221>  Artificial sequence
<222>  (1)..(1389)
<223>  SPD-m4-1BBL nucleotide sequence

<400>  6
atgctgccct ttctctccat gcttgtcttg cttgtacagc ccctgggaaa tctgggagca       60

gaaatgaaga gcctctcgca gagatcagta cccaacacct gcaccctagt catgtgtagc      120

ccaacagaga atggcctgcc tggtcgtgat ggacgggatg ggagagaagg tccacggggt      180

gagaagggtg atccaggttt gccaggacct atggggctct cagggttgca gggccctaca      240

ggtccagttg gacccaaagg agagaatggc tctgctggcg aacctggacc aaagggagaa      300

cgtggactaa gtggacctcc aggacttcca ggtattcctg gtccagctgg gaaagaaggt      360

ccctctggga agcaggggaa cataggacct caaggcaaac caggtcctaa aggagaggct      420

gggcccaaag gagaagtagg tgctcctggc atgcaaggat ctacaggggc aaaaggctcc      480

acaggcccca agggagaaag aggtgcccct ggtgtgcaag gagccccagg gaatgctgga      540

gcagcaggac ctgccggacc tgccggtcca cagggagctc caggttccag ggggccccca      600

ggactcaagg gggacagagg tgttcctgga gacagaggaa tcaaaggtga aagcgggctt      660

ccagacagtg ctgctctgag gcagcagatg gaggccttaa aaggaaaact acagcgtcta      720

gaggttgcct tctcccacta tcagaaagct gcattgttcc ctgatggccg caccgagcct      780

cggccagcgc tcacaatcac cacctcgccc aacctgggta cccgagagaa taatgcagac      840

caggtcaccc ctgtttccca cattggctgc cccaacacta cacaacaggg ctctcctgtg      900

ttcgccaagc tactggctaa aaaccaagca tcgttgtgca atacaactct gaactggcac      960

agccaagatg gagctgggag ctcataccta tctcaaggtc tgaggtacga agaagacaaa     1020

aaggagttgg tggtagacag tcccgggctc tactacgtat ttttggaact gaagctcagt     1080

ccaacattca caaacacagg ccacaaggtg cagggctggg tctctcttgt tttgcaagca     1140

aagcctcagg tagatgactt tgacaacttg gccctgacag tggaactgtt cccttgctcc     1200

atggagaaca agttagtgga ccgttcctgg agtcaactgt tgctcctgaa ggctggccac     1260

cgcctcagtg tgggtctgag ggcttatctg catggagccc aggatgcata cagagactgg     1320

gagctgtctt atcccaacac caccagcttt ggactctttc ttgtgaaacc cgacaaccca     1380

tgggaatga                                                             1389


<210>  7
<211>  463
<212>  PRT
<213>  Homo sapiens


<220>
<221>  Artificial sequence
<222>  (1)..(463)
<223>  SPD-h4-1BBL amino acid sequence

<400>  7

Met Leu Leu Phe Leu Leu Ser Ala Leu Val Leu Leu Thr Gln Pro Leu 
1               5                   10                  15      


Gly Tyr Leu Glu Ala Glu Met Lys Thr Tyr Ser His Arg Thr Met Pro 
            20                  25                  30          


Ser Ala Cys Thr Leu Val Met Cys Ser Ser Val Glu Ser Gly Leu Pro 
        35                  40                  45              


Gly Arg Asp Gly Arg Asp Gly Arg Glu Gly Pro Arg Gly Glu Lys Gly 
    50                  55                  60                  


Asp Pro Gly Leu Pro Gly Ala Ala Gly Gln Ala Gly Met Pro Gly Gln 
65                  70                  75                  80  


Ala Gly Pro Val Gly Pro Lys Gly Asp Asn Gly Ser Val Gly Glu Pro 
                85                  90                  95      


Gly Pro Lys Gly Asp Thr Gly Pro Ser Gly Pro Pro Gly Pro Pro Gly 
            100                 105                 110         


Val Pro Gly Pro Ala Gly Arg Glu Gly Pro Leu Gly Lys Gln Gly Asn 
        115                 120                 125             


Ile Gly Pro Gln Gly Lys Pro Gly Pro Lys Gly Glu Ala Gly Pro Lys 
    130                 135                 140                 


Gly Glu Val Gly Ala Pro Gly Met Gln Gly Ser Ala Gly Ala Arg Gly 
145                 150                 155                 160 


Leu Ala Gly Pro Lys Gly Glu Arg Gly Val Pro Gly Glu Arg Gly Val 
                165                 170                 175     


Pro Gly Asn Thr Gly Ala Ala Gly Ser Ala Gly Ala Met Gly Pro Gln 
            180                 185                 190         


Gly Ser Pro Gly Ala Arg Gly Pro Pro Gly Leu Lys Gly Asp Lys Gly 
        195                 200                 205             


Ile Pro Gly Asp Lys Gly Ala Lys Gly Glu Ser Gly Leu Pro Asp Val 
    210                 215                 220                 


Ala Ser Leu Arg Gln Gln Val Glu Ala Leu Gln Gly Gln Val Gln His 
225                 230                 235                 240 


Leu Gln Ala Ala Phe Ser Gln Tyr Lys Lys Val Glu Leu Phe Pro Asn 
                245                 250                 255     


Gly Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly 
            260                 265                 270         


Ser Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp 
        275                 280                 285             


Asp Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu 
    290                 295                 300                 


Val Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser 
305                 310                 315                 320 


Asp Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys 
                325                 330                 335     


Glu Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val 
            340                 345                 350         


Phe Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly 
        355                 360                 365             


Ser Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly 
    370                 375                 380                 


Ala Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu 
385                 390                 395                 400 


Ala Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser 
                405                 410                 415     


Ala Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg 
            420                 425                 430         


His Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg 
        435                 440                 445             


Val Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu 
    450                 455                 460             


<210>  8
<211>  1392
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Artificial sequence
<222>  (1)..(1392)
<223>  SPD-h4-1BBL nucleotide sequence

<400>  8
atgctgctct tcctcctctc tgcactggtc ctgctcacac agcccctggg ctacctggaa       60

gcagaaatga agacctactc ccacagaaca atgcccagtg cttgcaccct ggtcatgtgt      120

agctcagtgg agagtggcct gcctggtcgc gatggacggg atgggagaga gggccctcgg      180

ggcgagaagg gggacccagg tttgccagga gctgcagggc aagcagggat gcctggacaa      240

gctggcccag ttgggcccaa aggggacaat ggctctgttg gagaacctgg accaaaggga      300

gacactgggc caagtggacc tccaggacct cccggtgtgc ctggtccagc tggaagagaa      360

ggtcccctgg ggaagcaggg gaacatagga cctcagggca agccaggccc aaaaggagaa      420

gctgggccca aaggagaagt aggtgcccca ggcatgcagg gctcggcagg ggcaagaggc      480

ctcgcaggcc ctaagggaga gcgaggtgtc cctggtgagc gtggagtccc tggaaacaca      540

ggggcagcag ggtctgctgg agccatgggt ccccagggaa gtccaggtgc caggggaccc      600

ccgggattga agggggacaa aggcattcct ggagacaaag gagcaaaggg agaaagtggg      660

cttccagatg ttgcttctct gaggcagcag gttgaggcct tacagggaca agtacagcac      720

ctccaggctg ctttctctca gtataagaaa gttgagctct tcccaaatgg cctcgcctgc      780

ccctgggccg tgtccggggc tcgcgcctcg cccggctccg cggccagccc gagactccgc      840

gagggtcccg agctttcgcc cgacgatccc gccggcctct tggacctgcg gcagggcatg      900

tttgcgcagc tggtggccca aaatgttctg ctgatcgatg ggcccctgag ctggtacagt      960

gacccaggcc tggcaggcgt gtccctgacg gggggcctga gctacaaaga ggacacgaag     1020

gagctggtgg tggccaaggc tggagtctac tatgtcttct ttcaactaga gctgcggcgc     1080

gtggtggccg gcgagggctc aggctccgtt tcacttgcgc tgcacctgca gccactgcgc     1140

tctgctgctg gggccgccgc cctggctttg accgtggacc tgccacccgc ctcctccgag     1200

gctcggaact cggccttcgg tttccagggc cgcttgctgc acctgagtgc cggccagcgc     1260

ctgggcgtcc atcttcacac tgaggccagg gcacgccatg cctggcagct tacccagggc     1320

gccacagtct tgggactctt ccgggtgacc cccgaaatcc cagccggact cccttcaccg     1380

aggtcggaat aa                                                         1392


<210>  9
<211>  146
<212>  PRT
<213>  Mus musculus


<220>
<221>  PEPTIDE
<222>  (1)..(146)
<223>  mIL-21 amino acid sequence

<400>  9

Met Glu Arg Thr Leu Val Cys Leu Val Val Ile Phe Leu Gly Thr Val 
1               5                   10                  15      


Ala His Lys Ser Ser Pro Gln Gly Pro Asp Arg Leu Leu Ile Arg Leu 
            20                  25                  30          


Arg His Leu Ile Asp Ile Val Glu Gln Leu Lys Ile Tyr Glu Asn Asp 
        35                  40                  45              


Leu Asp Pro Glu Leu Leu Ser Ala Pro Gln Asp Val Lys Gly His Cys 
    50                  55                  60                  


Glu His Ala Ala Phe Ala Cys Phe Gln Lys Ala Lys Leu Lys Pro Ser 
65                  70                  75                  80  


Asn Pro Gly Asn Asn Lys Thr Phe Ile Ile Asp Leu Val Ala Gln Leu 
                85                  90                  95      


Arg Arg Arg Leu Pro Ala Arg Arg Gly Gly Lys Lys Gln Lys His Ile 
            100                 105                 110         


Ala Lys Cys Pro Ser Cys Asp Ser Tyr Glu Lys Arg Thr Pro Lys Glu 
        115                 120                 125             


Phe Leu Glu Arg Leu Lys Trp Leu Leu Gln Lys Met Ile His Gln His 
    130                 135                 140                 


Leu Ser 
145     


<210>  10
<211>  441
<212>  DNA
<213>  Mus musculus


<220>
<221>  exon
<222>  (1)..(441)
<223>  mIL-21 nucleotide sequence

<400>  10
atg gag agg acc ctt gtc tgt ctg gta gtc atc ttc ttg ggg aca gtg         48
Met Glu Arg Thr Leu Val Cys Leu Val Val Ile Phe Leu Gly Thr Val           
1               5                   10                  15                

gcc cat aaa tca agc ccc caa ggg cca gat cgc ctc ctg att aga ctt         96
Ala His Lys Ser Ser Pro Gln Gly Pro Asp Arg Leu Leu Ile Arg Leu           
            20                  25                  30                    

cgt cac ctt att gac att gtt gaa cag ctg aaa atc tat gaa aat gac        144
Arg His Leu Ile Asp Ile Val Glu Gln Leu Lys Ile Tyr Glu Asn Asp           
        35                  40                  45                        

ttg gat cct gaa ctt cta tca gct cca caa gat gta aag ggg cac tgt        192
Leu Asp Pro Glu Leu Leu Ser Ala Pro Gln Asp Val Lys Gly His Cys           
    50                  55                  60                            

gag cat gca gct ttt gcc tgt ttt cag aag gcc aaa ctc aag cca tca        240
Glu His Ala Ala Phe Ala Cys Phe Gln Lys Ala Lys Leu Lys Pro Ser           
65                  70                  75                  80            

aac cct gga aac aat aag aca ttc atc att gac ctc gtg gcc cag ctc        288
Asn Pro Gly Asn Asn Lys Thr Phe Ile Ile Asp Leu Val Ala Gln Leu           
                85                  90                  95                

agg agg agg ctg cct gcc agg agg gga gga aag aaa cag aag cac ata        336
Arg Arg Arg Leu Pro Ala Arg Arg Gly Gly Lys Lys Gln Lys His Ile           
            100                 105                 110                   

gct aaa tgc cct tcc tgt gat tcg tat gag aaa agg aca ccc aaa gaa        384
Ala Lys Cys Pro Ser Cys Asp Ser Tyr Glu Lys Arg Thr Pro Lys Glu           
        115                 120                 125                       

ttc cta gaa aga cta aaa tgg ctc ctt caa aag atg att cat cag cat        432
Phe Leu Glu Arg Leu Lys Trp Leu Leu Gln Lys Met Ile His Gln His           
    130                 135                 140                           

ctc tcc tga                                                            441
Leu Ser                                                                   
145                                                                       


<210>  11
<211>  162
<212>  PRT
<213>  Homo sapiens


<220>
<221>  PEPTIDE
<222>  (1)..(162)
<223>  hIL--21 amino acid sequence

<400>  11

Met Arg Ser Ser Pro Gly Asn Met Glu Arg Ile Val Ile Cys Leu Met 
1               5                   10                  15      


Val Ile Phe Leu Gly Thr Leu Val His Lys Ser Ser Ser Gln Gly Gln 
            20                  25                  30          


Asp Arg His Met Ile Arg Met Arg Gln Leu Ile Asp Ile Val Asp Gln 
        35                  40                  45              


Leu Lys Asn Tyr Val Asn Asp Leu Val Pro Glu Phe Leu Pro Ala Pro 
    50                  55                  60                  


Glu Asp Val Glu Thr Asn Cys Glu Trp Ser Ala Phe Ser Cys Phe Gln 
65                  70                  75                  80  


Lys Ala Gln Leu Lys Ser Ala Asn Thr Gly Asn Asn Glu Arg Ile Ile 
                85                  90                  95      


Asn Val Ser Ile Lys Lys Leu Lys Arg Lys Pro Pro Ser Thr Asn Ala 
            100                 105                 110         


Gly Arg Arg Gln Lys His Arg Leu Thr Cys Pro Ser Cys Asp Ser Tyr 
        115                 120                 125             


Glu Lys Lys Pro Pro Lys Glu Phe Leu Glu Arg Phe Lys Ser Leu Leu 
    130                 135                 140                 


Gln Lys Met Ile His Gln His Leu Ser Ser Arg Thr His Gly Ser Glu 
145                 150                 155                 160 


Asp Ser 
        


<210>  12
<211>  489
<212>  DNA
<213>  Homo sapiens


<220>
<221>  exon
<222>  (1)..(489)
<223>  hIL-21 nucleotide sequence

<400>  12
atg aga tcc agt cct ggc aac atg gag agg att gtc atc tgt ctg atg         48
Met Arg Ser Ser Pro Gly Asn Met Glu Arg Ile Val Ile Cys Leu Met           
1               5                   10                  15                

gtc atc ttc ttg ggg aca ctg gtc cac aaa tca agc tcc caa ggt caa         96
Val Ile Phe Leu Gly Thr Leu Val His Lys Ser Ser Ser Gln Gly Gln           
            20                  25                  30                    

gat cgc cac atg att aga atg cgt caa ctt ata gat att gtt gat cag        144
Asp Arg His Met Ile Arg Met Arg Gln Leu Ile Asp Ile Val Asp Gln           
        35                  40                  45                        

ctg aaa aat tat gtg aat gac ttg gtc cct gaa ttt ctg cca gct cca        192
Leu Lys Asn Tyr Val Asn Asp Leu Val Pro Glu Phe Leu Pro Ala Pro           
    50                  55                  60                            

gaa gat gta gag aca aac tgt gag tgg tca gct ttt tcc tgc ttt cag        240
Glu Asp Val Glu Thr Asn Cys Glu Trp Ser Ala Phe Ser Cys Phe Gln           
65                  70                  75                  80            

aag gcc caa cta aag tca gca aat aca gga aac aat gaa agg ata atc        288
Lys Ala Gln Leu Lys Ser Ala Asn Thr Gly Asn Asn Glu Arg Ile Ile           
                85                  90                  95                

aat gta tca att aaa aag ctg aag agg aaa cca cct tcc aca aat gca        336
Asn Val Ser Ile Lys Lys Leu Lys Arg Lys Pro Pro Ser Thr Asn Ala           
            100                 105                 110                   

ggg aga aga cag aaa cac aga cta aca tgc cct tca tgt gat tct tat        384
Gly Arg Arg Gln Lys His Arg Leu Thr Cys Pro Ser Cys Asp Ser Tyr           
        115                 120                 125                       

gag aaa aaa cca ccc aaa gaa ttc cta gaa aga ttc aaa tca ctt ctc        432
Glu Lys Lys Pro Pro Lys Glu Phe Leu Glu Arg Phe Lys Ser Leu Leu           
    130                 135                 140                           

caa aag atg att cat cag cat ctg tcc tct aga aca cac gga agt gaa        480
Gln Lys Met Ile His Gln His Leu Ser Ser Arg Thr His Gly Ser Glu           
145                 150                 155                 160           

gat tcc tag                                                            489
Asp Ser                                                                   
                                                                          


<210>  13
<211>  714
<212>  PRT
<213>  Homo sapiens


<220>
<221>  PEPTIDE
<222>  (1)..(714)

<400>  13

Met Leu Asp Thr Met Glu Ala Pro Gly His Ser Arg Gln Leu Leu Leu 
1               5                   10                  15      


Gln Leu Asn Asn Gln Arg Thr Lys Gly Phe Leu Cys Asp Val Ile Ile 
            20                  25                  30          


Val Val Gln Asn Ala Leu Phe Arg Ala His Lys Asn Val Leu Ala Ala 
        35                  40                  45              


Ser Ser Ala Tyr Leu Lys Ser Leu Val Val His Asp Asn Leu Leu Asn 
    50                  55                  60                  


Leu Asp His Asp Met Val Ser Pro Ala Val Phe Arg Leu Val Leu Asp 
65                  70                  75                  80  


Phe Ile Tyr Thr Gly Arg Leu Ala Asp Gly Ala Glu Ala Ala Ala Ala 
                85                  90                  95      


Ala Ala Val Ala Pro Gly Ala Glu Pro Ser Leu Gly Ala Val Leu Ala 
            100                 105                 110         


Ala Ala Ser Tyr Leu Gln Ile Pro Asp Leu Val Ala Leu Cys Lys Lys 
        115                 120                 125             


Arg Leu Lys Arg His Gly Lys Tyr Cys His Leu Arg Gly Gly Gly Gly 
    130                 135                 140                 


Gly Gly Gly Gly Tyr Ala Pro Tyr Gly Arg Pro Gly Arg Gly Leu Arg 
145                 150                 155                 160 


Ala Ala Thr Pro Val Ile Gln Ala Cys Tyr Pro Ser Pro Val Gly Pro 
                165                 170                 175     


Pro Pro Pro Pro Ala Ala Glu Pro Pro Ser Gly Pro Glu Ala Ala Val 
            180                 185                 190         


Asn Thr His Cys Ala Glu Leu Tyr Ala Ser Gly Pro Gly Pro Ala Ala 
        195                 200                 205             


Ala Leu Cys Ala Ser Glu Arg Arg Cys Ser Pro Leu Cys Gly Leu Asp 
    210                 215                 220                 


Leu Ser Lys Lys Ser Pro Pro Gly Ser Ala Ala Pro Glu Arg Pro Leu 
225                 230                 235                 240 


Ala Glu Arg Glu Leu Pro Pro Arg Pro Asp Ser Pro Pro Ser Ala Gly 
                245                 250                 255     


Pro Ala Ala Tyr Lys Glu Pro Pro Leu Ala Leu Pro Ser Leu Pro Pro 
            260                 265                 270         


Leu Pro Phe Gln Lys Leu Glu Glu Ala Ala Pro Pro Ser Asp Pro Phe 
        275                 280                 285             


Arg Gly Gly Ser Gly Ser Pro Gly Pro Glu Pro Pro Gly Arg Pro Asp 
    290                 295                 300                 


Gly Pro Ser Leu Leu Tyr Arg Trp Met Lys His Glu Pro Gly Leu Gly 
305                 310                 315                 320 


Ser Tyr Gly Asp Glu Leu Gly Arg Glu Arg Gly Ser Pro Ser Glu Arg 
                325                 330                 335     


Cys Glu Glu Arg Gly Gly Asp Ala Ala Val Ser Pro Gly Gly Pro Pro 
            340                 345                 350         


Leu Gly Leu Ala Pro Pro Pro Arg Tyr Pro Gly Ser Leu Asp Gly Pro 
        355                 360                 365             


Gly Ala Gly Gly Asp Gly Asp Asp Tyr Lys Ser Ser Ser Glu Glu Thr 
    370                 375                 380                 


Gly Ser Ser Glu Asp Pro Ser Pro Pro Gly Gly His Leu Glu Gly Tyr 
385                 390                 395                 400 


Pro Cys Pro His Leu Ala Tyr Gly Glu Pro Glu Ser Phe Gly Asp Asn 
                405                 410                 415     


Leu Tyr Val Cys Ile Pro Cys Gly Lys Gly Phe Pro Ser Ser Glu Gln 
            420                 425                 430         


Leu Asn Ala His Val Glu Ala His Val Glu Glu Glu Glu Ala Leu Tyr 
        435                 440                 445             


Gly Arg Ala Glu Ala Ala Glu Val Ala Ala Gly Ala Ala Gly Leu Gly 
    450                 455                 460                 


Pro Pro Phe Gly Gly Gly Gly Asp Lys Val Ala Gly Ala Pro Gly Gly 
465                 470                 475                 480 


Leu Gly Glu Leu Leu Arg Pro Tyr Arg Cys Ala Ser Cys Asp Lys Ser 
                485                 490                 495     


Tyr Lys Asp Pro Ala Thr Leu Arg Gln His Glu Lys Thr His Trp Leu 
            500                 505                 510         


Thr Arg Pro Tyr Pro Cys Thr Ile Cys Gly Lys Lys Phe Thr Gln Arg 
        515                 520                 525             


Gly Thr Met Thr Arg His Met Arg Ser His Leu Gly Leu Lys Pro Phe 
    530                 535                 540                 


Ala Cys Asp Ala Cys Gly Met Arg Phe Thr Arg Gln Tyr Arg Leu Thr 
545                 550                 555                 560 


Glu His Met Arg Ile His Ser Gly Glu Lys Pro Tyr Glu Cys Gln Val 
                565                 570                 575     


Cys Gly Gly Lys Phe Ala Gln Gln Arg Asn Leu Ile Ser His Met Lys 
            580                 585                 590         


Met His Ala Val Gly Gly Ala Ala Gly Ala Ala Gly Ala Leu Ala Gly 
        595                 600                 605             


Leu Gly Gly Leu Pro Gly Val Pro Gly Pro Asp Gly Lys Gly Lys Leu 
    610                 615                 620                 


Asp Phe Pro Glu Gly Val Phe Ala Val Ala Arg Leu Thr Ala Glu Gln 
625                 630                 635                 640 


Leu Ser Leu Lys Gln Gln Asp Lys Ala Ala Ala Ala Glu Leu Leu Ala 
                645                 650                 655     


Gln Thr Thr His Phe Leu His Asp Pro Lys Val Ala Leu Glu Ser Leu 
            660                 665                 670         


Tyr Pro Leu Ala Lys Phe Thr Ala Glu Leu Gly Leu Ser Pro Asp Lys 
        675                 680                 685             


Ala Ala Glu Val Leu Ser Gln Gly Ala His Leu Ala Ala Gly Pro Asp 
    690                 695                 700                 


Gly Arg Thr Ile Asp Arg Phe Ser Pro Thr 
705                 710                 


<210>  14
<211>  2145
<212>  DNA
<213>  Homo sapiens


<220>
<221>  exon
<222>  (1)..(2145)
<223>  HIC1 nucleotide sequence

<400>  14
atg ctg gac acg atg gag gcg ccc ggc cac tcc agg cag ctg ctg ctg         48
Met Leu Asp Thr Met Glu Ala Pro Gly His Ser Arg Gln Leu Leu Leu           
1               5                   10                  15                

cag ctc aac aac cag cgc acc aag ggc ttc ttg tgc gac gtg atc atc         96
Gln Leu Asn Asn Gln Arg Thr Lys Gly Phe Leu Cys Asp Val Ile Ile           
            20                  25                  30                    

gtg gtg cag aac gcc ctc ttc cgc gcg cac aag aac gtg ctg gcg gcc        144
Val Val Gln Asn Ala Leu Phe Arg Ala His Lys Asn Val Leu Ala Ala           
        35                  40                  45                        

agc agc gcc tac ctc aag tcc ctg gtg gtg cat gac aac ctg ctc aac        192
Ser Ser Ala Tyr Leu Lys Ser Leu Val Val His Asp Asn Leu Leu Asn           
    50                  55                  60                            

ctg gac cat gac atg gtg agc ccg gcc gtg ttc cgc ctg gtg ctg gac        240
Leu Asp His Asp Met Val Ser Pro Ala Val Phe Arg Leu Val Leu Asp           
65                  70                  75                  80            

ttc atc tac acc ggc cgc ctg gct gac ggc gca gag gcg gct gcg gcc        288
Phe Ile Tyr Thr Gly Arg Leu Ala Asp Gly Ala Glu Ala Ala Ala Ala           
                85                  90                  95                

gcg gcc gtg gcc ccg ggg gct gag ccg agc ctg ggc gcc gtg ctg gcc        336
Ala Ala Val Ala Pro Gly Ala Glu Pro Ser Leu Gly Ala Val Leu Ala           
            100                 105                 110                   

gcc gcc agc tac ctg cag atc ccc gac ctc gtg gcg ctg tgc aag aaa        384
Ala Ala Ser Tyr Leu Gln Ile Pro Asp Leu Val Ala Leu Cys Lys Lys           
        115                 120                 125                       

cgc ctc aag cgc cac ggc aag tac tgc cac ctg cgg ggc ggc ggc ggc        432
Arg Leu Lys Arg His Gly Lys Tyr Cys His Leu Arg Gly Gly Gly Gly           
    130                 135                 140                           

ggc ggc ggc ggc tac gcg ccc tat ggt cgg ccg ggc cgg ggc ctg cgg        480
Gly Gly Gly Gly Tyr Ala Pro Tyr Gly Arg Pro Gly Arg Gly Leu Arg           
145                 150                 155                 160           

gcc gcc acg ccg gtc atc cag gcc tgc tac ccg tcc cca gtc ggg cct        528
Ala Ala Thr Pro Val Ile Gln Ala Cys Tyr Pro Ser Pro Val Gly Pro           
                165                 170                 175               

ccg ccg ccg cct gcc gcg gag ccg ccc tcg ggc cca gag gcc gcg gtc        576
Pro Pro Pro Pro Ala Ala Glu Pro Pro Ser Gly Pro Glu Ala Ala Val           
            180                 185                 190                   

aac acg cac tgc gcc gag ctg tac gcg tcg gga ccc ggc ccg gcc gcc        624
Asn Thr His Cys Ala Glu Leu Tyr Ala Ser Gly Pro Gly Pro Ala Ala           
        195                 200                 205                       

gca ctc tgt gcc tcg gag cgc cgc tgc tcc cct ctt tgt ggc ctg gac        672
Ala Leu Cys Ala Ser Glu Arg Arg Cys Ser Pro Leu Cys Gly Leu Asp           
    210                 215                 220                           

ctg tcc aag aag agc ccg ccg ggc tcc gcg gcg cca gag cgg ccg ctg        720
Leu Ser Lys Lys Ser Pro Pro Gly Ser Ala Ala Pro Glu Arg Pro Leu           
225                 230                 235                 240           

gct gag cgc gag ctg ccc ccg cgc ccg gac agc cct ccc agc gcc ggc        768
Ala Glu Arg Glu Leu Pro Pro Arg Pro Asp Ser Pro Pro Ser Ala Gly           
                245                 250                 255               

ccc gcc gcc tac aag gag ccg cct ctc gcc ctg ccg tcg ctg ccg ccg        816
Pro Ala Ala Tyr Lys Glu Pro Pro Leu Ala Leu Pro Ser Leu Pro Pro           
            260                 265                 270                   

ctg ccc ttc cag aag ctg gag gag gcc gca ccg cct tcc gac cca ttt        864
Leu Pro Phe Gln Lys Leu Glu Glu Ala Ala Pro Pro Ser Asp Pro Phe           
        275                 280                 285                       

cgc ggc ggc agc ggc agc ccg gga ccc gag ccc ccc ggc cgc ccc gac        912
Arg Gly Gly Ser Gly Ser Pro Gly Pro Glu Pro Pro Gly Arg Pro Asp           
    290                 295                 300                           

ggg cct agt ctc ctc tat cgc tgg atg aag cac gag ccg ggc ctg ggt        960
Gly Pro Ser Leu Leu Tyr Arg Trp Met Lys His Glu Pro Gly Leu Gly           
305                 310                 315                 320           

agc tat ggc gac gag ctg ggc cgg gag cgc ggc tcc ccc agc gag cgc       1008
Ser Tyr Gly Asp Glu Leu Gly Arg Glu Arg Gly Ser Pro Ser Glu Arg           
                325                 330                 335               

tgc gaa gag cgt ggt ggg gac gcg gcc gtc tcg ccc ggg ggg ccc ccg       1056
Cys Glu Glu Arg Gly Gly Asp Ala Ala Val Ser Pro Gly Gly Pro Pro           
            340                 345                 350                   

ctc ggc ctg gcg ccg ccg ccg cgc tac cct ggc agc ctg gac ggg ccc       1104
Leu Gly Leu Ala Pro Pro Pro Arg Tyr Pro Gly Ser Leu Asp Gly Pro           
        355                 360                 365                       

ggc gcg ggc ggc gac ggc gac gac tac aag agc agc agc gag gag acc       1152
Gly Ala Gly Gly Asp Gly Asp Asp Tyr Lys Ser Ser Ser Glu Glu Thr           
    370                 375                 380                           

ggt agc agc gag gac ccc agc ccg cct ggc ggc cac ctc gag ggc tac       1200
Gly Ser Ser Glu Asp Pro Ser Pro Pro Gly Gly His Leu Glu Gly Tyr           
385                 390                 395                 400           

cca tgc ccg cac ctg gcc tat ggc gag ccc gag agc ttc ggt gac aac       1248
Pro Cys Pro His Leu Ala Tyr Gly Glu Pro Glu Ser Phe Gly Asp Asn           
                405                 410                 415               

ctg tac gtg tgc att ccg tgc ggc aag ggc ttc ccc agc tct gag cag       1296
Leu Tyr Val Cys Ile Pro Cys Gly Lys Gly Phe Pro Ser Ser Glu Gln           
            420                 425                 430                   

ctg aac gcg cac gtg gag gct cac gtg gag gag gag gaa gcg ctg tac       1344
Leu Asn Ala His Val Glu Ala His Val Glu Glu Glu Glu Ala Leu Tyr           
        435                 440                 445                       

ggc agg gcc gag gcg gcc gaa gtg gcc gct ggg gcc gcc ggc cta ggg       1392
Gly Arg Ala Glu Ala Ala Glu Val Ala Ala Gly Ala Ala Gly Leu Gly           
    450                 455                 460                           

ccc cct ttt gga ggc ggc ggg gac aag gtc gcc ggg gct ccg ggt ggc       1440
Pro Pro Phe Gly Gly Gly Gly Asp Lys Val Ala Gly Ala Pro Gly Gly           
465                 470                 475                 480           

ctg gga gag ctg ctg cgg ccc tac cgc tgc gcg tcg tgc gac aag agc       1488
Leu Gly Glu Leu Leu Arg Pro Tyr Arg Cys Ala Ser Cys Asp Lys Ser           
                485                 490                 495               

tac aag gac ccg gcc acg ctg cgg cag cac gag aag acg cac tgg ctg       1536
Tyr Lys Asp Pro Ala Thr Leu Arg Gln His Glu Lys Thr His Trp Leu           
            500                 505                 510                   

acc cgg ccc tac cca tgc acc atc tgc ggg aag aag ttc acg cag cgt       1584
Thr Arg Pro Tyr Pro Cys Thr Ile Cys Gly Lys Lys Phe Thr Gln Arg           
        515                 520                 525                       

ggg acc atg acg cgc cac atg cgc agc cac ctg ggc ctc aag ccc ttc       1632
Gly Thr Met Thr Arg His Met Arg Ser His Leu Gly Leu Lys Pro Phe           
    530                 535                 540                           

gcg tgc gac gcg tgc ggc atg cgg ttc acg cgc cag tac cgc ctc acg       1680
Ala Cys Asp Ala Cys Gly Met Arg Phe Thr Arg Gln Tyr Arg Leu Thr           
545                 550                 555                 560           

gag cac atg cgc atc cac tcg ggc gag aag ccc tac gag tgc cag gtg       1728
Glu His Met Arg Ile His Ser Gly Glu Lys Pro Tyr Glu Cys Gln Val           
                565                 570                 575               

tgc ggc ggc aag ttc gca cag caa cgc aac ctc atc agc cac atg aag       1776
Cys Gly Gly Lys Phe Ala Gln Gln Arg Asn Leu Ile Ser His Met Lys           
            580                 585                 590                   

atg cac gcc gtg ggg ggc gcg gcc ggc gcg gcc ggg gcg ctg gcg ggc       1824
Met His Ala Val Gly Gly Ala Ala Gly Ala Ala Gly Ala Leu Ala Gly           
        595                 600                 605                       

ttg ggg ggg ctc ccc ggc gtc ccc ggc ccc gac ggc aag ggc aag ctc       1872
Leu Gly Gly Leu Pro Gly Val Pro Gly Pro Asp Gly Lys Gly Lys Leu           
    610                 615                 620                           

gac ttc ccc gag ggc gtc ttt gct gtg gct cgc ctc acg gcc gag cag       1920
Asp Phe Pro Glu Gly Val Phe Ala Val Ala Arg Leu Thr Ala Glu Gln           
625                 630                 635                 640           

ctg agc ctg aag cag cag gac aag gcg gcc gcg gcc gag ctg ctg gcg       1968
Leu Ser Leu Lys Gln Gln Asp Lys Ala Ala Ala Ala Glu Leu Leu Ala           
                645                 650                 655               

cag acc acg cac ttc ctg cac gac ccc aag gtg gcg ctg gag agc ctc       2016
Gln Thr Thr His Phe Leu His Asp Pro Lys Val Ala Leu Glu Ser Leu           
            660                 665                 670                   

tac ccg ctg gcc aag ttc acg gcc gag ctg ggc ctc agc ccc gac aag       2064
Tyr Pro Leu Ala Lys Phe Thr Ala Glu Leu Gly Leu Ser Pro Asp Lys           
        675                 680                 685                       

gcg gcc gag gtg ctg agc cag ggc gct cac ctg gcg gcc ggg ccc gac       2112
Ala Ala Glu Val Leu Ser Gln Gly Ala His Leu Ala Ala Gly Pro Asp           
    690                 695                 700                           

ggc cgg acc atc gac cgt ttc tct ccc acc tag                           2145
Gly Arg Thr Ile Asp Arg Phe Ser Pro Thr                                   
705                 710                                                   


<210>  15
<211>  18
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Artificial sequence
<222>  (1)..(18)
<223>  forward primer for L025 gene

<400>  15
tatctagcaa tggaccgt                                                     18


<210>  16
<211>  19
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Artificial sequence
<222>  (1)..(19)
<223>  Reserse primer for L025 gene

<400>  16
ccgaaggtag tagcatgga                                                    19


<210>  17
<211>  21
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(21)
<223>  Forward primer for A46R and A47L genes

<400>  17
ttggctatta aacagtatgg a                                                 21


<210>  18
<211>  18
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(18)
<223>  Reserse primer for A46R and A47L genes

<400>  18
ggatcccgat aacaaatg                                                     18


<210>  19
<211>  25
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(25)
<223>  Forward primer for mB5R

<400>  19
atgaaaacga tttccgttgt tacgt                                             25


<210>  20
<211>  24
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(24)
<223>  Reserse primer for mB5R

<400>  20
tcacggtagc aatttatgga actt                                              24


<210>  21
<211>  25
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(25)
<223>  Forward primer for sB5R

<400>  21
atgaaaacga tttccgttgt tacgt                                             25


<210>  22
<211>  24
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(24)
<223>  Reserse primer for sB5R

<400>  22
tcacggtagc aatttatgga actt                                              24


<210>  23
<211>  20
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(20)
<223>  Forward primer for TK gene

<400>  23
gttatagtag ccgcactcga                                                   20


<210>  24
<211>  21
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(21)
<223>  Reserse primer for TK gene

<400>  24
atttcagctg aatatgaagg a                                                 21


<210>  25
<211>  20
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Artificial sequence
<222>  (1)..(20)
<223>  Forward primer for PD1 antibody gene

<400>  25
tcataaatac ccgagccacc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Homo sapiens


<220>
<221>  Artificial sequence
<222>  (1)..(20)
<223>  Reserse primer for PD1 antibody gene

<400>  26
acccattcaa gaccctttcc                                                   20


<210>  27
<211>  18
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(18)
<223>  Forward primer for control gene

<400>  27
tgttgttcgc tgctatga                                                     18


<210>  28
<211>  20
<212>  DNA
<213>  Vaccinia virus


<220>
<221>  Artificial sequence
<222>  (1)..(20)
<223>  Reserse primer for control gene

<400>  28
tggcacaacc atatcttgta                                                   20


