                         SEQUENCE LISTING

<110>  TAVOTEK BIOTHERAPEUTICS (HONG KONG) LIMITED
 
<120>  TRICYCLIC PEPTIDE AS PROTEIN BINDERS AND MODULATORS AND USES 
       THEREOF

<130>  TABI-005/01WO

<150>  US 62/871,991
<151>  2019-07-09

<160>  106   

<170>  PatentIn version 3.5

<210>  1
<211>  220
<212>  PRT
<213>  Escherichia virus M13

<400>  1

Ala Glu Thr Val Glu Ser Cys Leu Ala Lys Pro His Thr Glu Asn Ser 
1               5                   10                  15      


Phe Thr Asn Val Trp Lys Asp Asp Lys Thr Leu Asp Arg Tyr Ala Asn 
            20                  25                  30          


Tyr Glu Gly Cys Leu Trp Asn Ala Thr Gly Val Val Val Cys Thr Gly 
        35                  40                  45              


Asp Glu Thr Gln Cys Tyr Gly Thr Trp Val Pro Ile Gly Leu Ala Ile 
    50                  55                  60                  


Pro Glu Asn Glu Gly Gly Gly Ser Glu Gly Gly Gly Ser Glu Gly Gly 
65                  70                  75                  80  


Gly Ser Glu Gly Gly Gly Thr Lys Pro Pro Glu Tyr Gly Asp Thr Pro 
                85                  90                  95      


Ile Pro Gly Tyr Thr Tyr Ile Asn Pro Leu Asp Gly Thr Tyr Pro Pro 
            100                 105                 110         


Gly Thr Glu Gln Asn Pro Ala Asn Pro Asn Pro Ser Leu Glu Glu Ser 
        115                 120                 125             


Gln Pro Leu Asn Thr Phe Met Phe Gln Asn Asn Arg Phe Arg Asn Arg 
    130                 135                 140                 


Gln Gly Ala Leu Thr Val Tyr Thr Gly Thr Val Thr Gln Gly Thr Asp 
145                 150                 155                 160 


Pro Val Lys Thr Tyr Tyr Gln Tyr Thr Pro Val Ser Ser Lys Ala Met 
                165                 170                 175     


Tyr Asp Ala Tyr Trp Asn Gly Lys Phe Arg Asp Cys Ala Phe His Ser 
            180                 185                 190         


Gly Phe Asn Glu Asp Leu Phe Val Cys Glu Tyr Gln Gly Gln Ser Ser 
        195                 200                 205             


Asp Leu Pro Gln Pro Pro Val Asn Ala Pro Ser Gly 
    210                 215                 220 


<210>  2
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  engineered phage pIII protein free of cysteine

<400>  2

Ala Glu Thr Val Glu Ser Ser Leu Ala Lys Ser His Ile Glu Gly Ser 
1               5                   10                  15      


Phe Thr Asn Val Trp Lys Asp Asp Lys Thr Leu Asp Trp Tyr Ala Asn 
            20                  25                  30          


Tyr Glu Gly Ile Leu Trp Lys Ala Thr Gly Val Val Val Ile Thr Gly 
        35                  40                  45              


Asp Glu Thr Gln Val Tyr Ala Thr Trp Val Pro Ile Gly Leu Ala Ile 
    50                  55                  60                  


Pro Glu Asn Glu Gly Gly Gly Ser Glu Gly Gly Gly Ser Glu Gly Gly 
65                  70                  75                  80  


Gly Ser Glu Gly Gly Gly Thr Lys Pro Pro Glu Tyr Gly Asp Thr Pro 
                85                  90                  95      


Ile Pro Gly Tyr Ile Tyr Ile Asn Pro Leu Asp Gly Thr Tyr Pro Pro 
            100                 105                 110         


Gly Thr Glu Gln Asn Pro Ala Asn Pro Asn Pro Ser Leu Glu Glu Ser 
        115                 120                 125             


His Pro Leu Asn Thr Phe Met Phe Gln Asn Asn Arg Phe Arg Asn Arg 
    130                 135                 140                 


Gln Gly Ala Leu Thr Val Tyr Thr Gly Thr Val Thr Gln Gly Thr Asp 
145                 150                 155                 160 


Pro Val Lys Thr Tyr Tyr Gln Tyr Thr Pro Val Ser Ser Lys Ala Met 
                165                 170                 175     


Tyr Asp Ala Tyr Trp Asn Gly Lys Phe Arg Asp Val Ala Phe His Ser 
            180                 185                 190         


Gly Phe Asn Glu Asp Leu Leu Val Ala Glu Tyr Gln Gly Gln Ser Ser 
        195                 200                 205             


Tyr Leu Pro Gln Pro Pro Val Asn Ala Pro Ser Gly 
    210                 215                 220 


<210>  3
<211>  1414
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  Nucleotide sequence encoding cysteine-free pIII

<400>  3
cggccgaaac tgttgaaagt tctttagcaa aatcccatat agaaggttca tttactaacg       60

tctggaaaga cgacaaaact ttagattggt acgctaacta tgagggcatt ctgtggaagg      120

ctacaggcgt tgtagttatt actggtgacg aaactcaggt ttacgctaca tgggttccta      180

ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt tctgagggtg      240

gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct attccgggct      300

atatttatat caaccctctc gacggcactt atccgcctgg tactgagcaa aaccccgcta      360

atcctaatcc ttctcttgag gagtctcatc ctcttaatac tttcatgttt cagaataata      420

ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact caaggcactg      480

accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg tatgacgctt      540

actggaacgg taaattcaga gacgtcgctt tccattctgg ctttaatgag gatttacttg      600

ttgctgaata tcaaggccaa tcgtcttacc tgcctcaacc tcctgtcaat gctggcggcg      660

gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt ggcggttctg      720

agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt gattttgatt      780

atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat gaaaacgcgc      840

tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt gctgctatcg      900

atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact ggtgattttg      960

ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct ttaatgaata     1020

atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct tttgtctttg     1080

gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta ttccgtggtg     1140

tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg tttgctaaca     1200

tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt tattattgcg     1260

tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc ttaaaaaggg     1320

cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg ggcttaactc     1380

aattcttgtg ggttatctct ctgatattag cgct                                 1414


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  PCR primer Tricyclic-F

<400>  4
gcaatgacct gatagccttt gtaga                                             25


<210>  5
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  PCR primer Tricyclic-R


<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (37)..(38)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (40)..(41)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (46)..(47)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (52)..(53)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (58)..(59)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (64)..(65)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (67)..(68)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (70)..(71)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (73)..(74)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (76)..(77)
<223>  n is a, c, g, or t

<400>  5
catgtttcgg ccgagccacc cccacamnnm nnmnnmnnmn ngcamnnmnn mnnmnnmnna       60

camnnmnnmn nmnnmnngca ggcagagtga gaatagaaag gtaccactaa agg             113


<210>  6
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  PCR primer M13KE-E

<400>  6
attcgcaatt cctttagtgg tacct                                             25


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  PCR primer M13KE-F

<400>  7
cgttctagct gataaattaa tgcc                                              24


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  PCR primer M13KE-Amplicon-F

<400>  8
cacctcgaaa gcaagctgat a                                                 21


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  PCR primer M13KE-Amplicon-R

<400>  9
ttgtcgtctt tccagacgtt ag                                                22


<210>  10
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  10

Cys Val Asp Arg Val Gly Cys Phe Pro Gly Arg Ala Cys Gln Val Arg 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  11
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  11

Cys Gly Lys Pro Asp Pro Cys Ser Ser Asp Leu Ala Cys Arg Pro Leu 
1               5                   10                  15      


Phe Arg Cys 
            


<210>  12
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  12

Cys Ser Pro Ser Pro Ala Cys Val Thr Asn Asp Val Cys Trp Arg Pro 
1               5                   10                  15      


Gly Val Cys 
            


<210>  13
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  13

Cys Gly Asn Gln Pro Trp Cys Gln Trp Ala Pro Thr Cys Gln Glu Trp 
1               5                   10                  15      


Val Asp Cys 
            


<210>  14
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  14

Cys Glu Arg Val Leu Asp Cys Pro Pro Gly Ile Pro Cys Arg Asp Arg 
1               5                   10                  15      


Met Ser Cys 
            


<210>  15
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  15

Cys Thr Lys Asp Gly Pro Cys Leu Arg Pro Pro Tyr Cys Thr Asp Tyr 
1               5                   10                  15      


Gly Gly Cys 
            


<210>  16
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  16

Cys Leu Gly Trp Gln Lys Cys Ala Ser Ser Thr Leu Cys Val Gln Arg 
1               5                   10                  15      


Met Thr Cys 
            


<210>  17
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  17

Cys Glu Thr Ala Gly Val Cys Trp Thr Lys Val Gly Cys Met Thr Thr 
1               5                   10                  15      


Ser Glu Cys 
            


<210>  18
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  18

Cys Tyr Thr Leu Pro Thr Cys Tyr Gly Ser Pro Leu Cys Arg Ser Leu 
1               5                   10                  15      


Pro Pro Cys 
            


<210>  19
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  19

Cys Ala Glu Arg Ala Pro Cys Phe Pro Ala Gly Arg Cys Glu Gly Phe 
1               5                   10                  15      


Leu Pro Cys 
            


<210>  20
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  20

Cys Leu Gly Met Gln Pro Cys Val Arg Pro Leu Glu Cys Gln Ser Pro 
1               5                   10                  15      


Val Ile Cys 
            


<210>  21
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  21

Cys Asp Leu Glu Pro Ser Cys Gln Arg Ser Thr Arg Cys Gln Trp Met 
1               5                   10                  15      


Arg Asp Cys 
            


<210>  22
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  22

Cys Val Gly Val Leu Asp Cys Thr Leu Ala Ala Lys Cys Ser Arg Ala 
1               5                   10                  15      


Asn Thr Cys 
            


<210>  23
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  23

Cys Asp Gly Phe Asp Tyr Cys Leu Pro Lys Ala Pro Cys Tyr Pro Thr 
1               5                   10                  15      


Val Tyr Cys 
            


<210>  24
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  24

Cys Glu Ala Thr Ile Arg Cys Leu Leu Gln Glu Lys Cys Gly Gln Ser 
1               5                   10                  15      


Asp Arg Cys 
            


<210>  25
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  25

Cys Tyr Asn Arg Ala Asp Cys Ser Thr Asp Leu Leu Cys Arg Thr Gln 
1               5                   10                  15      


Ser Tyr Cys 
            


<210>  26
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  26

Cys Asp Tyr Ala Ala Arg Cys Ala Ser Pro Gly Ser Cys Arg Gly Ala 
1               5                   10                  15      


Trp Val Cys 
            


<210>  27
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  27

Cys Leu Glu Asp Gln Thr Cys Thr Gly Tyr Gly Met Cys Ala Arg Gly 
1               5                   10                  15      


Ser Val Cys 
            


<210>  28
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  28

Cys Gly Asn Val Gly Thr Cys Thr Glu Thr Lys Ser Cys Gln Tyr Pro 
1               5                   10                  15      


Ile Ala Cys 
            


<210>  29
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  29

Cys Lys Val Asp Asn Gly Cys Ser Ser Arg Ala Lys Cys Gly Gly Ser 
1               5                   10                  15      


Trp Val Cys 
            


<210>  30
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  30

Cys Pro Gly Gly Gln Trp Cys Pro Val Lys Arg Ala Cys Thr Trp Ala 
1               5                   10                  15      


Thr Gly Cys 
            


<210>  31
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  31

Cys Asn Thr Val Gly Ser Cys Leu Asp Leu Asn Ala Cys Arg Val Pro 
1               5                   10                  15      


Pro Arg Cys 
            


<210>  32
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  32

Cys Pro Leu Ala Gln Tyr Cys Arg Ala Leu Pro Ser Cys Gly Leu Glu 
1               5                   10                  15      


Trp Arg Cys 
            


<210>  33
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  33

Cys Pro Ser Ala Glu Lys Cys Thr Gly Arg Gln Gln Cys Tyr Trp Ser 
1               5                   10                  15      


Ser Lys Cys 
            


<210>  34
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  34

Cys Pro Thr Arg Pro Ser Cys Thr Thr Thr Leu Pro Cys Ser Trp Ala 
1               5                   10                  15      


Gln Gln Cys 
            


<210>  35
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  35

Cys Trp Gly Asp Gly Trp Cys Tyr Thr Asn Pro Gly Cys Ser Arg Glu 
1               5                   10                  15      


Arg Leu Cys 
            


<210>  36
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  36

Cys Thr Val Pro Tyr Glu Cys Gly Gly Leu Asp Pro Cys Pro Trp Lys 
1               5                   10                  15      


Ala Gln Cys 
            


<210>  37
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  37

Cys Phe Asn Glu Thr Trp Cys Thr Met Gly Arg Val Cys Pro Arg Gly 
1               5                   10                  15      


Trp Glu Cys 
            


<210>  38
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  38

Cys Pro Asp Ala Trp Leu Cys Pro Trp Ala Tyr Met Cys Asp Gly Arg 
1               5                   10                  15      


Ala Trp Cys 
            


<210>  39
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  39

Cys Lys Ile Thr Val Thr Cys Ala Ala Phe Pro Pro Cys Gln Ala Gln 
1               5                   10                  15      


Glu Arg Cys 
            


<210>  40
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  40

Cys Asn Asp Ala Trp Gly Cys Asp Ser Ser Ala Thr Cys Leu Pro Gly 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  41
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  41

Cys Gly Phe Ala Gly Pro Cys Gly Ala Lys Arg Gln Cys Ala Ile Gln 
1               5                   10                  15      


Thr Glu Cys 
            


<210>  42
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  42

Cys Pro Ile Tyr Glu Ser Cys Gly Arg Leu Ser Ser Cys Glu Pro Gly 
1               5                   10                  15      


Leu Ser Cys 
            


<210>  43
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  43

Cys Trp Glu Pro Leu Ala Cys Thr Arg Ile Ala Pro Cys Thr Glu Thr 
1               5                   10                  15      


Gln Gly Cys 
            


<210>  44
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  44

Cys Gln Asp Gly Tyr Ala Cys Ser Trp Lys Phe Glu Cys Arg Pro Pro 
1               5                   10                  15      


Ala Trp Cys 
            


<210>  45
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  45

Cys Gly Ser Glu Gln Arg Cys Tyr Thr Thr Pro Gly Cys Leu Gly Gln 
1               5                   10                  15      


Ser Glu Cys 
            


<210>  46
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  46

Cys Tyr Thr Glu Gly Pro Cys Ser Leu Asp Arg Thr Cys Ala Gly Arg 
1               5                   10                  15      


Ala Ser Cys 
            


<210>  47
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  47

Cys Gln Gly Ala Leu Gln Cys Ser Pro Leu Ala Val Cys Ile Ser Thr 
1               5                   10                  15      


Asp Tyr Cys 
            


<210>  48
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  48

Cys Phe Ala Leu Ser Arg Cys Glu Ser Gln Pro Pro Cys Ala Ala Pro 
1               5                   10                  15      


Thr Ser Cys 
            


<210>  49
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  49

Cys Leu Gly Val Glu Gly Cys Thr Gln Met Asp Pro Cys Ala Pro Ser 
1               5                   10                  15      


Met Gly Cys 
            


<210>  50
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  50

Cys Leu Gly Asn Leu Thr Cys Thr Gly Gly Ser Gly Cys Asn Gly Ser 
1               5                   10                  15      


Asp Thr Cys 
            


<210>  51
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  51

Cys Gly Leu Asp Gly Asp Cys Arg Val Leu Lys Ser Cys Trp Leu Thr 
1               5                   10                  15      


Arg Met Cys 
            


<210>  52
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  52

Cys Gln Val Met Glu Leu Cys Gly Ser Leu Lys Arg Cys Val Pro Ser 
1               5                   10                  15      


Arg Phe Cys 
            


<210>  53
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  53

Cys Gly Lys Phe Asp Leu Cys Gln Arg Ala Thr Glu Cys Gly Ala Thr 
1               5                   10                  15      


Ser Thr Cys 
            


<210>  54
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  54

Cys Ala Gly Leu Gly Val Cys Asp Pro Ser Pro Thr Cys Arg Trp Tyr 
1               5                   10                  15      


Gln Arg Cys 
            


<210>  55
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  55

Cys Asp Leu Gly Leu Ser Cys Asp Thr Leu Ser Gly Cys Arg Gly Ala 
1               5                   10                  15      


Trp Val Cys 
            


<210>  56
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate PD-1 binder hit

<400>  56

Cys Pro Trp Thr Val Glu Cys Pro Pro Asp Ala Thr Cys Glu Asn Ser 
1               5                   10                  15      


Ser Tyr Cys 
            


<210>  57
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  57

Cys Gly Leu Pro Trp Asp Cys Arg Val Asp Ser Gln Cys Met Thr Val 
1               5                   10                  15      


Glu Glu Cys 
            


<210>  58
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  58

Cys Leu Leu Glu Met Asn Cys Pro Pro Asp Ala Thr Cys Thr Gly Pro 
1               5                   10                  15      


Val Leu Cys 
            


<210>  59
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  59

Cys Asn Trp Ser Ala Asp Cys Trp Asp Thr Ala Glu Cys Leu Glu Thr 
1               5                   10                  15      


Ser Pro Cys 
            


<210>  60
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  60

Cys Asp Asn Val Leu Ser Cys Trp Gly Asp Asp Tyr Cys Ala Ala Ser 
1               5                   10                  15      


Tyr Glu Cys 
            


<210>  61
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  61

Cys Tyr Met Trp Trp Asn Cys Glu Thr Val Glu Ala Cys Ala Gln Glu 
1               5                   10                  15      


Pro Trp Cys 
            


<210>  62
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  62

Cys Thr Ser Trp Glu Trp Cys Val Asp Val Ser Thr Cys Ser Thr Glu 
1               5                   10                  15      


Ala Glu Cys 
            


<210>  63
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  63

Cys Glu Gly Met Ser Glu Cys Ala Thr Leu Asp Leu Cys Leu Ala Ile 
1               5                   10                  15      


Arg Gln Cys 
            


<210>  64
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  64

Cys Asp Ala Asn Glu Val Cys Leu Ala Pro Tyr Asn Cys Leu Pro Arg 
1               5                   10                  15      


Gly Glu Cys 
            


<210>  65
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  65

Cys Glu Ser Trp Glu Ser Cys Glu Ala Leu Ser Leu Cys Trp Thr Ser 
1               5                   10                  15      


Glu Met Cys 
            


<210>  66
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  66

Cys Val Gly Ala Tyr Glu Cys Asn Glu Phe Gly Val Cys Glu Leu Ile 
1               5                   10                  15      


Asn Asn Cys 
            


<210>  67
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  67

Cys Glu Thr Tyr Ala Asn Cys Glu Pro Ala Pro Leu Cys Phe Ala Ala 
1               5                   10                  15      


Trp Asp Cys 
            


<210>  68
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  68

Cys Phe Pro Gly Glu Ser Cys Arg Asp Val Gly Arg Cys Ala Gly Ser 
1               5                   10                  15      


Ser Gln Cys 
            


<210>  69
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  69

Cys Ser Asn Leu Ser Asp Cys Met Asp Gln Leu Thr Cys Ala Leu Gly 
1               5                   10                  15      


Ser Ser Cys 
            


<210>  70
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  70

Cys Tyr Ser Ala Val Glu Cys Val Arg Ala Leu Asn Cys Ala Met Asp 
1               5                   10                  15      


Pro Thr Cys 
            


<210>  71
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  71

Cys Gly Glu Ala Pro Gly Cys Ser Thr Val Asp Ser Cys Leu Phe Ala 
1               5                   10                  15      


Asp Leu Cys 
            


<210>  72
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  72

Cys Asp Trp Glu Gln Ala Cys Thr Glu Ala Met Glu Cys Leu Val Thr 
1               5                   10                  15      


Gly Ala Cys 
            


<210>  73
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  73

Cys Met Thr Ser Met Ser Cys Gly Asp Gly Trp Ser Cys Ala Gln Ser 
1               5                   10                  15      


Leu Met Cys 
            


<210>  74
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  74

Cys Glu Ser Met Ala Glu Cys Leu Pro Asn Leu Met Cys Ala Ala Trp 
1               5                   10                  15      


Arg Thr Cys 
            


<210>  75
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  75

Cys Thr Gly Gln Trp Pro Cys Ile Ala Glu Leu Glu Cys Lys Ser Leu 
1               5                   10                  15      


Trp Asp Cys 
            


<210>  76
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  76

Cys Ser Val Ser Ser Gly Cys Thr Ala Met Asp Trp Cys Trp Asp Ser 
1               5                   10                  15      


Thr Ser Cys 
            


<210>  77
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  77

Cys Ile Ser Pro Gly Ser Cys Ser Pro Thr Met Asn Cys Leu Thr Asp 
1               5                   10                  15      


Trp Glu Cys 
            


<210>  78
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  78

Cys Met Gln Ile Asp Glu Cys Ala Pro Leu Leu Gly Cys Ser Ala Thr 
1               5                   10                  15      


Met Ala Cys 
            


<210>  79
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  79

Cys Trp Met Glu Ala Pro Cys Gly Pro Lys Gln Asn Cys Gly Gln Thr 
1               5                   10                  15      


Asn Leu Cys 
            


<210>  80
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  80

Cys Thr Asp Val Glu Asn Cys Val Pro Trp Trp Glu Cys Gly Val Glu 
1               5                   10                  15      


Trp Gln Cys 
            


<210>  81
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  81

Cys Lys Thr Asp Pro Trp Cys Pro Pro Gly Pro Glu Cys Arg Met Gly 
1               5                   10                  15      


Ser Ile Cys 
            


<210>  82
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  82

Cys Ile Asp Thr Thr Thr Cys Glu Thr Thr Gly Arg Cys Ser Asn Met 
1               5                   10                  15      


Pro Ala Cys 
            


<210>  83
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  83

Cys Asp Pro Leu Trp Thr Cys Trp Leu Pro Glu Arg Cys Asp Arg Glu 
1               5                   10                  15      


Val Ala Cys 
            


<210>  84
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate BCL-2 binder hit

<400>  84

Cys Glu Ser Leu Arg Ser Cys Leu Asn Ser Thr Leu Cys Ala Trp Thr 
1               5                   10                  15      


Asp Ala Cys 
            


<210>  85
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  85

Cys Asn Pro Val Asp Pro Cys Arg Pro Gln Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  86
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  86

Cys Val Glu Ala Asp Pro Cys Gly Ser Trp Glu Pro Cys His Pro Met 
1               5                   10                  15      


Ala Pro Cys 
            


<210>  87
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  87

Cys Asn Pro Val Asp Pro Cys His Pro Arg Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  88
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  88

Cys Asn Pro Val Asp Pro Cys His Leu Gln Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  89
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  89

Cys Asn Pro Val Asp Pro Cys Tyr Pro Gln Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  90
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  90

Cys Asn Pro Val Asp Pro Cys Arg Pro Met Thr Ser Cys Lys Thr Trp 
1               5                   10                  15      


Ser Pro Cys 
            


<210>  91
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  91

Cys Asn Pro Val Asp Pro Cys His Ser Gln Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  92
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  92

Cys Asn Pro Gln Val Pro Cys Arg Pro Met Thr Ser Cys Lys Thr Trp 
1               5                   10                  15      


Ser Pro Cys 
            


<210>  93
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  93

Cys Ile Pro Leu Tyr Ser Cys His Pro Met Ser Pro Cys Lys Pro His 
1               5                   10                  15      


Trp Gly Cys 
            


<210>  94
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  94

Cys Asn Pro Val Asp Pro Cys Gln Pro Gln Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  95
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  95

Cys Trp His His Pro Met Cys Gly Ser His Ala Ala Cys Leu Arg Ser 
1               5                   10                  15      


Gly Glu Cys 
            


<210>  96
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  96

Cys Asn Pro Val Asp Pro Cys His Gln Gln Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  97
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  97

Cys Asp Val Val Gly Thr Cys Met Thr His Arg Pro Cys His Gln Phe 
1               5                   10                  15      


Val Lys Cys 
            


<210>  98
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  98

Cys Ser Ser Val Glu Val Cys Ser Ser Lys Pro Tyr Cys Val Arg Thr 
1               5                   10                  15      


Gly Gln Cys 
            


<210>  99
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  99

Cys Asn Pro Val Asp Pro Cys His Pro Leu Gly Gly Cys Trp Gln Gln 
1               5                   10                  15      


Thr Thr Cys 
            


<210>  100
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  100

Cys Ala Pro Val Lys Gln Cys His Pro Met Val Pro Cys Glu Leu Gly 
1               5                   10                  15      


Pro Ser Cys 
            


<210>  101
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  101

Cys His Pro Met Arg Pro Cys Leu Tyr Ser Asp Gln Cys Ala Gln Pro 
1               5                   10                  15      


Gln Pro Cys 
            


<210>  102
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  candidate TNF alpha hits

<400>  102

Cys Tyr Pro Gln Val Pro Cys Arg Pro Met Thr Ser Cys Lys Thr Trp 
1               5                   10                  15      


Ser Pro Cys 
            


<210>  103
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>  
<223>  consensus sequence encoding semi-random peptide


<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (13)..(14)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (16)..(17)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (19)..(20)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (25)..(26)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (28)..(29)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (31)..(32)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (37)..(38)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (43)..(44)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (46)..(47)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (49)..(50)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (52)..(53)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (55)..(56)
<223>  n is a, c, g, or t

<400>  103
gcctgcnnkn nknnknnknn ktgtnnknnk nnknnknnkt gcnnknnknn knnknnktgt       60

gggggtggct cg                                                           72


<210>  104
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  consensus sequence for semi-random peptide with linker peptide


<220>
<221>  misc_feature
<222>  (3)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(13)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (15)..(19)
<223>  Xaa can be any naturally occurring amino acid

<400>  104

Ala Cys Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Cys Gly Gly Gly Ser 
            20                  


<210>  105
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  consensus sequence for semi-random peptide


<220>
<221>  misc_feature
<222>  (3)..(7)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (9)..(13)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (15)..(19)
<223>  Xaa can be any naturally occurring amino acid

<400>  105

Ala Cys Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Cys 
            20  


<210>  106
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>  
<223>  linker peptide

<400>  106

Gly Gly Gly Ser 
1               


