<110>    CHUNG ANG University industry Academic Cooperation Foundation

<120>    Novel Lactococcus chungangensis CAU 1447 strain

<130>    OP20-0035/PCT

<150>    KR 10-2019-0082691
<151>    2019-07-09

<160>    27

<170>    KoPatentIn 3.0

<210>    1
<211>    1482
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    16S rRNA gene sequence of Lactococcus chungangensis CAU 1447
         (1,482bp)


<400>    1
acaaagacac cagagcttgc tctaccgtct ttgttgagta gcgaacgggt gagtaacgcg         60

tgggtaacct gccttatagc gggggataac tattggaaac gatagctaat accgcataac        120

aatagagatt gcataatctt tatttgaaag tgccaactgg tacactatga gatggacccg        180

cgttgtatta gctagttggt agtgtaatgg actaccaagg cgatgataca tagccgacct        240

gagagggtga tcggccacat tgggactgag acacggccca aactcctacg ggaggcagca        300

gtagggaatc ttcggcaatg gacgaaagtc tgaccgagca acgccgcgtg agtgaagaag        360

gttttcggat cgtaaaactc tgttgttaga gaagaacgtt gattagagtg gaaaattaat        420

caagtgacgg tatctaacca gaaagggacg gctaactacg tgccagcagc cgcggtaata        480

cgtaggtccc gagcgttgtc cggatttatt gggcgtaaag cgagcgcagg tggtttaata        540

agtctgatgt aaaaggcagt ggctcaacca ttgtgtgcat tggaaactgt tagacttgag        600

tgcagtagag gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa        660

caccggtggc gaaagcggct ctctggactg tcactgacac tgaggctcga aagcgtgggt        720

agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgagtgc tagttgtttg        780

gggctatcca gccctaagtg acgcagcaaa cgcattaagc actccgcctg gggagtacga        840

ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt        900

ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atactcgtgc tattcctaga        960

gataggaagt tccttcggga cacgggatac aggtggtgca tggttgtcgt cagctcgtgt       1020

cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt       1080

aagttgggca ctctagcgag actgccggta ataaaccgga ggaaggtggg gatgacgtca       1140

aatcatcatg ccccttatga cctgggctac acacgtgcta caatggttgg tacaacgagt       1200

cgcaagacag tgatgtcaag ctaatgtctt aaagccaatc tcagttcgga ttgtaggctg       1260

caactcgcct acatgaagtc ggaatcgcta gtaatcgcgg atcagctcgc cgcggtgaat       1320

acgttgccgc gccttgtaca caccgcccgt gactccacga cagtttgtaa taccctaagc       1380

cggtgagcta accttttagg aggcagccgt ctaaggtagg atagatgatt ggggtgaagt       1440

cgtaacatgg tagccgtaac ggaaggtgcg gctggtccac ct                          1482


<210>    2
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for 16S rRNA gene


<400>    2
agagtttgat cctggctcag                                                     20


<210>    3
<211>    16
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for 16S rRNA gene


<400>    3
aggaggtgat ccagcc                                                         16


<210>    4
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for ADH


<400>    4
ggtttgtgac ccaggtatgg t                                                   21


<210>    5
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for ADH


<400>    5
gttccaagca ccaccaact                                                      19


<210>    6
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for ALDH


<400>    6
gttgccatca ttgacacttg gt                                                  22


<210>    7
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for ALDH


<400>    7
aggaatgttc caagcaccac                                                     20


<210>    8
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for IL-1beta


<400>    8
caccttcttt tccttcatct ttg                                                 23


<210>    9
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for IL-1beta


<400>    9
gtcgttgctt gtctctcctt gta                                                 23


<210>    10
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for TNF-alpha


<400>    10
gtcgtagcaa accaccaagc                                                     20


<210>    11
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for TNF-alpha


<400>    11
tgtgggtgag gagcacatag                                                     20


<210>    12
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for IFN-gamma


<400>    12
tctggaggaa ctggcaaaag                                                     20


<210>    13
<211>    24
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for IFN-gamma


<400>    13
ttcaagactt caaagagtct gagg                                                24


<210>    14
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for IL-10


<400>    14
gggaagcaac tgaaacttcg                                                     20


<210>    15
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for IL-10


<400>    15
atcatggaag gagcaacctg                                                     20


<210>    16
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for COX-2


<400>    16
aatcgctgta caagcagtgg                                                     20


<210>    17
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for COX-2


<400>    17
gcagccattt ctttctctcc                                                     20


<210>    18
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for iNOS


<400>    18
ccttgttcag ctacgccttc                                                     20


<210>    19
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for iNOS


<400>    19
ggtatgcccg agttctttca                                                     20


<210>    20
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for SOD


<400>    20
ggtccacctc gaactacttt atg                                                 23


<210>    21
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for SOD


<400>    21
ggtgatcagg aacatggaat ct                                                  22


<210>    22
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for TLR-4


<400>    22
gatgcctctc ttgcatctgg                                                     20


<210>    23
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for TLR-4


<400>    23
tcatgaggga ttttgctgag a                                                   21


<210>    24
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for NF-kB


<400>    24
cagagctggc agagagactg                                                     20


<210>    25
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for NF-kB


<400>    25
tacgaaggag actgccactg                                                     20


<210>    26
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Forward primer for GAPDH


<400>    26
caagttcaac ggcacagtca a                                                   21


<210>    27
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Reverse primer for GAPDH


<400>    27
ggtgaagacg ccagtagact c                                                   21
