                         б

<110>  йѧԺ΢о
 
<120>  ĽĻ༭ϵͳӦ

<130>  P2020TC1155

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  1

Gly Gly Ser Gly Gly Leu Leu Asp Pro Gly Thr Pro Met Asp Ala Asp 
1               5                   10                  15      


Leu Val Ala Ser Ser Thr Val Val Trp Glu Gln Asp Ala Asp Pro Phe 
            20                  25                  30          


Ala Gly Thr Ala Asp Asp Phe Pro Ala Phe Asn Glu Glu Glu Leu Ala 
        35                  40                  45              


Trp Leu Met Glu Leu Leu Pro Gln Gly Gly Ser Gly Gly Leu Leu Asp 
    50                  55                  60                  


Pro Gly Thr Pro Met Asp Ala Asp Leu Val Ala Ser Ser Thr Val Val 
65                  70                  75                  80  


Trp Glu Gln Asp Ala Asp Pro Phe Ala Gly Thr Ala Asp Asp Phe Pro 
                85                  90                  95      


Ala Phe Asn Glu Glu Glu Leu Ala Trp Leu Met Glu Leu Leu Pro Gln 
            100                 105                 110         


Ala Arg Gly Gly Ser Gly Gly Leu Leu Asp Pro Gly Thr Pro Met Asp 
        115                 120                 125             


Ala Asp Leu Val Ala Ser Ser Thr Val Val Trp Glu Gln Asp Ala Asp 
    130                 135                 140                 


Pro Phe Ala Gly Thr Ala Asp Asp Phe Pro Ala Phe Asn Glu Glu Glu 
145                 150                 155                 160 


Leu Ala Trp Leu Met Glu Leu Leu Pro Gln Gly Gly Ser Gly Gly Leu 
                165                 170                 175     


Leu Asp Pro Gly Thr Pro Met Asp Ala Asp Leu Val Ala Ser Ser Thr 
            180                 185                 190         


Val Val Trp Glu Gln Asp Ala Asp Pro Phe Ala Gly Thr Ala Asp Asp 
        195                 200                 205             


Phe Pro Ala Phe Asn Glu Glu Glu Leu Ala Trp Leu Met Glu Leu Leu 
    210                 215                 220                 


Pro Gln Ala Arg Gly Gly Ser Gly Gly Leu Leu Asp Pro Gly Thr Pro 
225                 230                 235                 240 


Met Asp Ala Asp Leu Val Ala Ser Ser Thr Val Val Trp Glu Gln Asp 
                245                 250                 255     


Ala Asp Pro Phe Ala Gly Thr Ala Asp Asp Phe Pro Ala Phe Asn Glu 
            260                 265                 270         


Glu Glu Leu Ala Trp Leu Met Glu Leu Leu Pro Gln Gly Gly Ser Gly 
        275                 280                 285             


Gly Leu Leu Asp Pro Gly Thr Pro Met Asp Ala Asp Leu Val Ala Ser 
    290                 295                 300                 


Ser Thr Val Val Trp Glu Gln Asp Ala Asp Pro Phe Ala Gly Thr Ala 
305                 310                 315                 320 


Asp Asp Phe Pro Ala Phe Asn Glu Glu Glu Leu Ala Trp Leu Met Glu 
                325                 330                 335     


Leu Leu Pro Gln Ala Arg Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu 
            340                 345                 350         


Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu 
        355                 360                 365             


Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp 
    370                 375                 380                 


Ala Leu Asp Asp Phe Asp Leu Asp Met Leu Ala Arg Gly Ser Asp Ala 
385                 390                 395                 400 


Leu Asp Asp Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp 
                405                 410                 415     


Phe Asp Leu Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu 
            420                 425                 430         


Asp Met Leu Gly Ser Asp Ala Leu Asp Asp Phe Asp Leu Asp Met Leu 
        435                 440                 445             


Ala Arg 
    450 


<210>  2
<211>  1853
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein

<400>  2

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala 
1               5                   10                  15      


Ala Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser 
            20                  25                  30          


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
        35                  40                  45              


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
    50                  55                  60                  


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
65                  70                  75                  80  


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
                85                  90                  95      


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
            100                 105                 110         


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
        115                 120                 125             


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
    130                 135                 140                 


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
145                 150                 155                 160 


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
                165                 170                 175     


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
            180                 185                 190         


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
        195                 200                 205             


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
    210                 215                 220                 


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
225                 230                 235                 240 


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
                245                 250                 255     


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
            260                 265                 270         


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
        275                 280                 285             


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
    290                 295                 300                 


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
305                 310                 315                 320 


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
                325                 330                 335     


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
            340                 345                 350         


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
        355                 360                 365             


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
    370                 375                 380                 


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
385                 390                 395                 400 


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
                405                 410                 415     


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
            420                 425                 430         


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
        435                 440                 445             


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
    450                 455                 460                 


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
465                 470                 475                 480 


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
                485                 490                 495     


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
            500                 505                 510         


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
        515                 520                 525             


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
    530                 535                 540                 


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
545                 550                 555                 560 


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
                565                 570                 575     


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
            580                 585                 590         


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
        595                 600                 605             


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
    610                 615                 620                 


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
625                 630                 635                 640 


Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr 
                645                 650                 655     


Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg 
            660                 665                 670         


Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg 
        675                 680                 685             


Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly 
    690                 695                 700                 


Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr 
705                 710                 715                 720 


Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser 
                725                 730                 735     


Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys 
            740                 745                 750         


Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met 
        755                 760                 765             


Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn 
    770                 775                 780                 


Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg 
785                 790                 795                 800 


Ile Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His 
                805                 810                 815     


Pro Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr 
            820                 825                 830         


Leu Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn 
        835                 840                 845             


Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu 
    850                 855                 860                 


Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn 
865                 870                 875                 880 


Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met 
                885                 890                 895     


Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg 
            900                 905                 910         


Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu 
        915                 920                 925             


Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile 
    930                 935                 940                 


Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr 
945                 950                 955                 960 


Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys 
                965                 970                 975     


Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val 
            980                 985                 990         


Arg Glu Ile Asn Asn Tyr His His  Ala His Asp Ala Tyr  Leu Asn Ala 
        995                 1000                 1005             


Val Val  Gly Thr Ala Leu Ile  Lys Lys Tyr Pro Lys  Leu Glu Ser 
    1010                 1015                 1020             


Glu Phe  Val Tyr Gly Asp Tyr  Lys Val Tyr Asp Val  Arg Lys Met 
    1025                 1030                 1035             


Ile Ala  Lys Ser Glu Gln Glu  Ile Gly Lys Ala Thr  Ala Lys Tyr 
    1040                 1045                 1050             


Phe Phe  Tyr Ser Asn Ile Met  Asn Phe Phe Lys Thr  Glu Ile Thr 
    1055                 1060                 1065             


Leu Ala  Asn Gly Glu Ile Arg  Lys Arg Pro Leu Ile  Glu Thr Asn 
    1070                 1075                 1080             


Gly Glu  Thr Gly Glu Ile Val  Trp Asp Lys Gly Arg  Asp Phe Ala 
    1085                 1090                 1095             


Thr Val  Arg Lys Val Leu Ser  Met Pro Gln Val Asn  Ile Val Lys 
    1100                 1105                 1110             


Lys Thr  Glu Val Gln Thr Gly  Gly Phe Ser Lys Glu  Ser Ile Leu 
    1115                 1120                 1125             


Pro Lys  Arg Asn Ser Asp Lys  Leu Ile Ala Arg Lys  Lys Asp Trp 
    1130                 1135                 1140             


Asp Pro  Lys Lys Tyr Gly Gly  Phe Asp Ser Pro Thr  Val Ala Tyr 
    1145                 1150                 1155             


Ser Val  Leu Val Val Ala Lys  Val Glu Lys Gly Lys  Ser Lys Lys 
    1160                 1165                 1170             


Leu Lys  Ser Val Lys Glu Leu  Leu Gly Ile Thr Ile  Met Glu Arg 
    1175                 1180                 1185             


Ser Ser  Phe Glu Lys Asn Pro  Ile Asp Phe Leu Glu  Ala Lys Gly 
    1190                 1195                 1200             


Tyr Lys  Glu Val Lys Lys Asp  Leu Ile Ile Lys Leu  Pro Lys Tyr 
    1205                 1210                 1215             


Ser Leu  Phe Glu Leu Glu Asn  Gly Arg Lys Arg Met  Leu Ala Ser 
    1220                 1225                 1230             


Ala Gly  Glu Leu Gln Lys Gly  Asn Glu Leu Ala Leu  Pro Ser Lys 
    1235                 1240                 1245             


Tyr Val  Asn Phe Leu Tyr Leu  Ala Ser His Tyr Glu  Lys Leu Lys 
    1250                 1255                 1260             


Gly Ser  Pro Glu Asp Asn Glu  Gln Lys Gln Leu Phe  Val Glu Gln 
    1265                 1270                 1275             


His Lys  His Tyr Leu Asp Glu  Ile Ile Glu Gln Ile  Ser Glu Phe 
    1280                 1285                 1290             


Ser Lys  Arg Val Ile Leu Ala  Asp Ala Asn Leu Asp  Lys Val Leu 
    1295                 1300                 1305             


Ser Ala  Tyr Asn Lys His Arg  Asp Lys Pro Ile Arg  Glu Gln Ala 
    1310                 1315                 1320             


Glu Asn  Ile Ile His Leu Phe  Thr Leu Thr Asn Leu  Gly Ala Pro 
    1325                 1330                 1335             


Ala Ala  Phe Lys Tyr Phe Asp  Thr Thr Ile Asp Arg  Lys Arg Tyr 
    1340                 1345                 1350             


Thr Ser  Thr Lys Glu Val Leu  Asp Ala Thr Leu Ile  His Gln Ser 
    1355                 1360                 1365             


Ile Thr  Gly Leu Tyr Glu Thr  Arg Ile Asp Leu Ser  Gln Leu Gly 
    1370                 1375                 1380             


Gly Asp  Lys Arg Pro Ala Ala  Thr Lys Lys Ala Gly  Gln Ala Lys 
    1385                 1390                 1395             


Lys Lys  Lys Pro Arg Gly Gly  Ser Gly Gly Leu Leu  Asp Pro Gly 
    1400                 1405                 1410             


Thr Pro  Met Asp Ala Asp Leu  Val Ala Ser Ser Thr  Val Val Trp 
    1415                 1420                 1425             


Glu Gln  Asp Ala Asp Pro Phe  Ala Gly Thr Ala Asp  Asp Phe Pro 
    1430                 1435                 1440             


Ala Phe  Asn Glu Glu Glu Leu  Ala Trp Leu Met Glu  Leu Leu Pro 
    1445                 1450                 1455             


Gln Gly  Gly Ser Gly Gly Leu  Leu Asp Pro Gly Thr  Pro Met Asp 
    1460                 1465                 1470             


Ala Asp  Leu Val Ala Ser Ser  Thr Val Val Trp Glu  Gln Asp Ala 
    1475                 1480                 1485             


Asp Pro  Phe Ala Gly Thr Ala  Asp Asp Phe Pro Ala  Phe Asn Glu 
    1490                 1495                 1500             


Glu Glu  Leu Ala Trp Leu Met  Glu Leu Leu Pro Gln  Ala Arg Gly 
    1505                 1510                 1515             


Gly Ser  Gly Gly Leu Leu Asp  Pro Gly Thr Pro Met  Asp Ala Asp 
    1520                 1525                 1530             


Leu Val  Ala Ser Ser Thr Val  Val Trp Glu Gln Asp  Ala Asp Pro 
    1535                 1540                 1545             


Phe Ala  Gly Thr Ala Asp Asp  Phe Pro Ala Phe Asn  Glu Glu Glu 
    1550                 1555                 1560             


Leu Ala  Trp Leu Met Glu Leu  Leu Pro Gln Gly Gly  Ser Gly Gly 
    1565                 1570                 1575             


Leu Leu  Asp Pro Gly Thr Pro  Met Asp Ala Asp Leu  Val Ala Ser 
    1580                 1585                 1590             


Ser Thr  Val Val Trp Glu Gln  Asp Ala Asp Pro Phe  Ala Gly Thr 
    1595                 1600                 1605             


Ala Asp  Asp Phe Pro Ala Phe  Asn Glu Glu Glu Leu  Ala Trp Leu 
    1610                 1615                 1620             


Met Glu  Leu Leu Pro Gln Ala  Arg Gly Gly Ser Gly  Gly Leu Leu 
    1625                 1630                 1635             


Asp Pro  Gly Thr Pro Met Asp  Ala Asp Leu Val Ala  Ser Ser Thr 
    1640                 1645                 1650             


Val Val  Trp Glu Gln Asp Ala  Asp Pro Phe Ala Gly  Thr Ala Asp 
    1655                 1660                 1665             


Asp Phe  Pro Ala Phe Asn Glu  Glu Glu Leu Ala Trp  Leu Met Glu 
    1670                 1675                 1680             


Leu Leu  Pro Gln Gly Gly Ser  Gly Gly Leu Leu Asp  Pro Gly Thr 
    1685                 1690                 1695             


Pro Met  Asp Ala Asp Leu Val  Ala Ser Ser Thr Val  Val Trp Glu 
    1700                 1705                 1710             


Gln Asp  Ala Asp Pro Phe Ala  Gly Thr Ala Asp Asp  Phe Pro Ala 
    1715                 1720                 1725             


Phe Asn  Glu Glu Glu Leu Ala  Trp Leu Met Glu Leu  Leu Pro Gln 
    1730                 1735                 1740             


Ala Arg  Gly Ser Asp Ala Leu  Asp Asp Phe Asp Leu  Asp Met Leu 
    1745                 1750                 1755             


Gly Ser  Asp Ala Leu Asp Asp  Phe Asp Leu Asp Met  Leu Gly Ser 
    1760                 1765                 1770             


Asp Ala  Leu Asp Asp Phe Asp  Leu Asp Met Leu Gly  Ser Asp Ala 
    1775                 1780                 1785             


Leu Asp  Asp Phe Asp Leu Asp  Met Leu Ala Arg Gly  Ser Asp Ala 
    1790                 1795                 1800             


Leu Asp  Asp Phe Asp Leu Asp  Met Leu Gly Ser Asp  Ala Leu Asp 
    1805                 1810                 1815             


Asp Phe  Asp Leu Asp Met Leu  Gly Ser Asp Ala Leu  Asp Asp Phe 
    1820                 1825                 1830             


Asp Leu  Asp Met Leu Gly Ser  Asp Ala Leu Asp Asp  Phe Asp Leu 
    1835                 1840                 1845             


Asp Met  Leu Ala Arg 
    1850             


<210>  3
<211>  5562
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fusion protein coding sequence

<400>  3
atggccccta agaagaagag aaaggtcggt attcacggcg ttcctgcggc gatggacaag       60

aagtatagta ttggtctgga cattgggacg aattccgttg gctgggccgt gatcaccgat      120

gagtacaagg tcccttccaa gaagtttaag gttctgggga acaccgatcg gcacagcatc      180

aagaagaatc tcattggagc cctcctgttc gactcaggcg agaccgccga agcaacaagg      240

ctcaagagaa ccgcaaggag acggtataca agaaggaaga ataggatctg ctacctgcag      300

gagattttca gcaacgaaat ggcgaaggtg gacgattcgt tctttcatag attggaggag      360

agtttcctcg tcgaggaaga taagaagcac gagaggcatc ctatctttgg caacattgtc      420

gacgaggttg cctatcacga aaagtacccc acaatctatc atctgcggaa gaagcttgtg      480

gactcgactg ataaggcgga ccttagattg atctacctcg ctctggcaca catgattaag      540

ttcaggggcc attttctgat cgagggggat cttaacccgg acaatagcga tgtggacaag      600

ttgttcatcc agctcgtcca aacctacaat cagctctttg aggaaaaccc aattaatgct      660

tcaggcgtcg acgccaaggc gatcctgtct gcacgccttt caaagtctcg ccggcttgag      720

aacttgatcg ctcaactccc gggcgaaaag aagaacggct tgttcgggaa tctcattgca      780

ctttcgttgg ggctcacacc aaacttcaag agtaattttg atctcgctga ggacgcaaag      840

ctgcagcttt ccaaggacac ttatgacgat gacctggata accttttggc ccaaatcggc      900

gatcagtacg cggacttgtt cctcgccgcg aagaatttgt cggacgcgat cctcctgagt      960

gatattctcc gcgtgaacac cgagattaca aaggccccgc tctcggcgag tatgatcaag     1020

cgctatgacg agcaccatca ggatctgacc cttttgaagg ctttggtccg gcagcaactc     1080

ccagagaagt acaaggaaat cttctttgat caatccaaga acggctacgc tggttatatt     1140

gacggcgggg catcgcagga ggaattctac aagtttatca agccaattct ggagaagatg     1200

gatggcacag aggaactcct ggtgaagctc aatagggagg accttttgcg gaagcaaaga     1260

actttcgata acggcagcat ccctcaccag attcatctcg gggagctgca cgccatcctg     1320

agaaggcagg aagacttcta cccctttctt aaggataacc gggagaagat cgaaaagatt     1380

ctgacgttca gaattccgta ctatgtcgga ccactcgccc ggggtaattc cagatttgcg     1440

tggatgacca gaaagagcga ggaaaccatc acaccttgga acttcgagga agtggtcgat     1500

aagggcgctt ccgcacagag cttcattgag cgcatgacaa attttgacaa gaacctgcct     1560

aatgagaagg tccttcccaa gcattccctc ctgtacgagt atttcactgt ttataacgaa     1620

ctcacgaagg tgaagtatgt gaccgaggga atgcgcaagc ccgccttcct gagcggcgag     1680

caaaagaagg cgatcgtgga ccttttgttt aagaccaatc ggaaggtcac agttaagcag     1740

ctcaaggagg actacttcaa gaagattgaa tgcttcgatt ccgttgagat cagcggcgtg     1800

gaagacaggt ttaacgcgtc actggggact taccacgatc tcctgaagat cattaaggat     1860

aaggacttct tggacaacga ggaaaatgag gatatcctcg aagacattgt cctgactctt     1920

acgttgtttg aggataggga aatgatcgag gaacgcttga agacgtatgc ccatctcttc     1980

gatgacaagg ttatgaagca gctcaagaga agaagataca ccggatgggg aaggctgtcc     2040

cgcaagctta tcaatggcat tagagacaag caatcaggga agacaatcct tgactttttg     2100

aagtctgatg gcttcgcgaa caggaatttt atgcagctga ttcacgatga ctcacttact     2160

ttcaaggagg atatccagaa ggctcaagtg tcgggacaag gtgacagtct gcacgagcat     2220

atcgccaacc ttgcgggatc tcctgcaatc aagaagggta ttctgcagac agtcaaggtt     2280

gtggatgagc ttgtgaaggt catgggacgg cataagcccg agaacatcgt tattgagatg     2340

gccagagaaa atcagaccac acaaaagggt cagaagaact cgagggagcg catgaagcgc     2400

atcgaggaag gcattaagga gctggggagt cagatcctta aggagcaccc ggtggaaaac     2460

acgcagttgc aaaatgagaa gctctatctg tactatctgc aaaatggcag ggatatgtat     2520

gtggaccagg agttggatat taaccgcctc tcggattacg acgtcgatca tatcgttcct     2580

cagtccttcc ttaaggatga cagcattgac aataaggttc tcaccaggtc cgacaagaac     2640

cgcgggaagt ccgataatgt gcccagcgag gaagtcgtta agaagatgaa gaactactgg     2700

aggcaacttt tgaatgccaa gttgatcaca cagaggaagt ttgataacct cactaaggcc     2760

gagcgcggag gtctcagcga actggacaag gcgggcttca ttaagcggca actggttgag     2820

actagacaga tcacgaagca cgtggcgcag attctcgatt cacgcatgaa cacgaagtac     2880

gatgagaatg acaagctgat ccgggaagtg aaggtcatca ccttgaagtc aaagctcgtt     2940

tctgacttca ggaaggattt ccaattttat aaggtgcgcg agatcaacaa ttatcaccat     3000

gctcatgacg catacctcaa cgctgtggtc ggaacagcat tgattaagaa gtacccgaag     3060

ctcgagtccg aattcgtgta cggtgactat aaggtttacg atgtgcgcaa gatgatcgcc     3120

aagtcagagc aggaaattgg caaggccact gcgaagtatt tcttttactc taacattatg     3180

aatttcttta agactgagat cacgctggct aatggcgaaa tccggaagag accacttatt     3240

gagaccaacg gcgagacagg ggaaatcgtg tgggacaagg ggagggattt cgccacagtc     3300

cgcaaggttc tctctatgcc tcaagtgaat attgtcaaga agactgaagt ccagacgggc     3360

gggttctcaa aggaatctat tctgcccaag cggaactcgg ataagcttat cgccagaaag     3420

aaggactggg acccgaagaa gtatggaggt ttcgactcac caacggtggc ttactctgtc     3480

ctggttgtgg caaaggtgga gaagggaaag tcaaagaagc tcaagtctgt caaggagctc     3540

ctgggtatca ccattatgga gaggtccagc ttcgaaaaga atccgatcga ttttctcgag     3600

gcgaagggat ataaggaagt gaagaaggac ctgatcatta agcttccaaa gtacagtctt     3660

ttcgagttgg aaaacggcag gaagcgcatg ttggcttccg caggagagct ccagaagggt     3720

aacgagcttg ctttgccgtc caagtatgtg aacttcctct atctggcatc ccactacgag     3780

aagctcaagg gcagcccaga ggataacgaa cagaagcaac tgtttgtgga gcaacacaag     3840

cattatcttg acgagatcat tgaacagatt tcggagttca gtaagcgcgt catcctcgcc     3900

gacgcgaatt tggataaggt tctctcagcc tacaacaagc accgggacaa gcctatcaga     3960

gagcaggcgg aaaatatcat tcatctcttc accctgacaa accttggggc tcccgctgca     4020

ttcaagtatt ttgacactac gattgatcgg aagagataca cttctacgaa ggaggtgctg     4080

gatgcaaccc ttatccacca atcgattact ggcctctacg agacgcggat cgacttgagt     4140

cagctcgggg gggataagag accagcggca accaagaagg caggacaagc gaagaagaag     4200

aagcctaggg gcggaagcgg cggcctcctg gacccaggaa ccccgatgga cgccgatctc     4260

gtggcgtcca gcacagtggt ctgggagcag gatgcggatc cattcgcggg aaccgcggac     4320

gatttcccag ccttcaacga ggaggagctg gcctggctga tggagctcct gccacagggc     4380

ggctcaggcg gcctcctgga tcctggaaca ccgatggacg cggatctcgt ggcctcatct     4440

accgtcgtct gggagcagga cgccgatcct ttcgccggca cagcggacga tttcccggcc     4500

ttcaatgagg aggagctcgc gtggctgatg gagctgctcc cacaggctag gggcggaagc     4560

ggcggcctcc tggacccagg aaccccgatg gacgccgatc tcgtggcgtc cagcacagtg     4620

gtctgggagc aggatgcgga tccattcgcg ggaaccgcgg acgatttccc agccttcaac     4680

gaggaggagc tggcctggct gatggagctc ctgccacagg gcggctcagg cggcctcctg     4740

gatcctggaa caccgatgga cgcggatctc gtggcctcat ctaccgtcgt ctgggagcag     4800

gacgccgatc ctttcgccgg cacagcggac gatttcccgg ccttcaatga ggaggagctc     4860

gcgtggctga tggagctgct cccacaggct aggggcggaa gcggcggcct cctggaccca     4920

ggaaccccga tggacgccga tctcgtggcg tccagcacag tggtctggga gcaggatgcg     4980

gatccattcg cgggaaccgc ggacgatttc ccagccttca acgaggagga gctggcctgg     5040

ctgatggagc tcctgccaca gggcggctca ggcggcctcc tggatcctgg aacaccgatg     5100

gacgcggatc tcgtggcctc atctaccgtc gtctgggagc aggacgccga tcctttcgcc     5160

ggcacagcgg acgatttccc ggccttcaat gaggaggagc tcgcgtggct gatggagctg     5220

ctcccacagg ctaggggctc agacgccctg gatgatttcg acctcgatat gctgggctca     5280

gatgccctgg acgactttga cctcgacatg ctcggctctg acgcgctcga cgactttgac     5340

ctcgatatgc tcggttccga tgccctggac gacttcgacc tcgacatgct cgctaggggc     5400

tcagacgccc tggatgattt cgacctcgat atgctgggct cagatgccct ggacgacttt     5460

gacctcgaca tgctcggctc tgacgcgctc gacgactttg acctcgatat gctcggttcc     5520

gatgccctgg acgacttcga cctcgacatg ctcgctaggt ag                        5562


<210>  4
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA scaffold

<400>  4
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgctttt ttt                                               83


<210>  5
<211>  381
<212>  DNA
<213>  Oryza sativa

<400>  5
aaggaatctt taaacatacg aacagatcac ttaaagttct tctgaagcaa cttaaagtta       60

tcaggcatgc atggatcttg gaggaatcag atgtgcagtc agggaccata gcacaagaca      120

ggcgtcttct actggtgcta ccagcaaatg ctggaagccg ggaacactgg gtacgtcgga      180

aaccacgtga tgtgaagaag taagataaac tgtaggagaa aagcatttcg tagtgggcca      240

tgaagccttt caggacatgt attgcagtat gggccggccc attacgcaat tggacgacaa      300

caaagactag tattagtacc acctcggcta tccacataga tcaaagctga tttaaaagag      360

ttgtgcagat gatccgtggc a                                                381


<210>  6
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  VP64-CO

<400>  6
cctaggggct cagacgccct ggatgatttc gacctcgata tgctgggctc agatgccctg       60

gacgactttg acctcgacat gctcggctct gacgcgctcg acgactttga cctcgatatg      120

ctcggttccg atgccctgga cgacttcgac ctcgacatgc tcgctagc                   168


<210>  7
<211>  348
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2TAL

<400>  7
cctaggggcg gaagcggcgg cctcctggac ccaggaaccc cgatggacgc cgatctcgtg       60

gcgtccagca cagtggtctg ggagcaggat gcggatccat tcgcgggaac cgcggacgat      120

ttcccagcct tcaacgagga ggagctggcc tggctgatgg agctcctgcc acagggcggc      180

tcaggcggcc tcctggatcc tggaacaccg atggacgcgg atctcgtggc ctcatctacc      240

gtcgtctggg agcaggacgc cgatcctttc gccggcacag cggacgattt cccggccttc      300

aatgaggagg agctcgcgtg gctgatggag ctgctcccac aggctagc                   348


