                         SEQUENCE LISTING

<110>  Astute Medical, Inc.
 
<120>  ANTIBODIES AND ASSAYS FOR CCL14

<130>  A105893 1580WO

<150>  U.S. 62/869,803
<151>  2019-07-02

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  143
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  5H2 heavy chain variable region

<400>  1

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Val Lys Glu Ser Glu Gly Gly Leu Phe Lys Pro 
            20                  25                  30          


Thr Asp Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Tyr 
        35                  40                  45              


Ser Gly Ala Ile Asn Trp Val Arg Gln Ala Pro Gly Glu Gly Leu Gln 
    50                  55                  60                  


Tyr Ile Gly Trp Ile Ser Asp Val Gly Ser Ala Tyr Tyr Ala Ser Trp 
65                  70                  75                  80  


Ala Lys Ser Arg Ser Thr Ile Thr Arg Asn Thr Asn Glu Asn Thr Val 
                85                  90                  95      


Thr Leu Lys Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe 
            100                 105                 110         


Cys Ala Arg Gly Asp Gly Ser Ser Gly Asn Tyr Trp Val Thr Asp Ile 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gln Pro Lys 
    130                 135                 140             


<210>  2
<211>  132
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  5K3 light chain variable region

<400>  2

Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Arg Cys Ala Val Val Leu Thr Gln Thr Ala Ser Pro 
            20                  25                  30          


Val Ser Ala Pro Val Gly Gly Thr Val Thr Ile Asn Cys Gln Ala Ser 
        35                  40                  45              


Glu Ser Ile Ser Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Asp Leu Glu Cys Ala Asp Ala Ala Thr Tyr Ser Cys Gln Glu 
            100                 105                 110         


Tyr Leu Ser Asp Asn Thr Phe Gly Gly Gly Thr Glu Val Val Val Lys 
        115                 120                 125             


Gly Asp Pro Val 
    130         


<210>  3
<211>  143
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  8H3 heavy chain variable region

<400>  3

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Val Lys Glu Ser Glu Gly Gly Leu Phe Lys Pro 
            20                  25                  30          


Thr Asp Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Tyr 
        35                  40                  45              


Ser Gly Ala Ile Asn Trp Val Arg Gln Ala Pro Gly Glu Gly Leu Gln 
    50                  55                  60                  


Tyr Ile Gly Trp Ile Ser Asp Val Gly Ser Ala Tyr Tyr Ala Ser Trp 
65                  70                  75                  80  


Ala Lys Ser Arg Ser Thr Ile Thr Arg Asn Thr Asn Glu Asn Thr Val 
                85                  90                  95      


Thr Leu Lys Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe 
            100                 105                 110         


Cys Ala Arg Gly Asp Gly Ser Ser Gly Asn Tyr Trp Val Thr Asp Ile 
        115                 120                 125             


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gln Pro Lys 
    130                 135                 140             


<210>  4
<211>  132
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  8K3 light chain variable region

<400>  4

Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Arg Cys Ala Val Val Leu Thr Gln Thr Ala Ser Pro 
            20                  25                  30          


Val Ser Ala Pro Val Gly Gly Thr Val Thr Ile Asn Cys Gln Ala Ser 
        35                  40                  45              


Glu Ser Ile Ser Ser Arg Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Asp Leu Glu Cys Ala Asp Ala Ala Thr Tyr Ser Cys Gln Glu 
            100                 105                 110         


Tyr Leu Ser Asp Asn Thr Phe Gly Gly Gly Thr Glu Val Val Val Lys 
        115                 120                 125             


Gly Asp Pro Val 
    130         


<210>  5
<211>  138
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  9H3 heavy chain variable region

<400>  5

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Val Lys Glu Ser Glu Gly Gly Leu Phe Lys Pro 
            20                  25                  30          


Thr Asp Thr Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Asn 
        35                  40                  45              


Ser Tyr Gly Val Asn Trp Val Arg Gln Ala Pro Gly Asn Gly Leu Glu 
    50                  55                  60                  


Tyr Ile Gly Thr Val Gly Ser Ser Gly Ser Ala Tyr Tyr Ala Ser Trp 
65                  70                  75                  80  


Ala Lys Ser Arg Ser Thr Ile Thr Arg Asn Thr Asn Leu Asn Thr Val 
                85                  90                  95      


Thr Leu Lys Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe 
            100                 105                 110         


Cys Ala Arg Gly Leu Ile Ala Thr Met Ser Ile Trp Gly Gln Gly Thr 
        115                 120                 125             


Leu Val Thr Val Ser Ser Gly Gln Pro Lys 
    130                 135             


<210>  6
<211>  136
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  9K2 light chain variable region

<400>  6

Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Thr Phe Ala Gln Val Leu Thr Gln Thr Ala Ser Ser 
            20                  25                  30          


Val Ser Ala Ala Val Gly Gly Thr Val Thr Ile Ser Cys Gln Ser Ser 
        35                  40                  45              


Gln Ser Val His Ser Asn Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro 
    50                  55                  60                  


Gly Gln Pro Pro Lys Gln Leu Ile Tyr Leu Ala Ser Thr Leu Ala Ser 
65                  70                  75                  80  


Gly Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Gln Phe Thr 
                85                  90                  95      


Leu Thr Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Thr Tyr Tyr Cys 
            100                 105                 110         


Ala Gly Gly Tyr Ser Gly Met Ile Phe Thr Phe Gly Gly Gly Thr Glu 
        115                 120                 125             


Val Val Val Lys Gly Asp Pro Val 
    130                 135     


<210>  7
<211>  144
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  14H1 heavy chain variable region

<400>  7

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Leu Glu Glu Ser Gly Gly Asp Leu Val Lys Pro 
            20                  25                  30          


Gly Ala Phe Leu Thr Leu Thr Cys Thr Ala Ser Gly Phe Ser Phe Ser 
        35                  40                  45              


Gly Ser Asp Tyr Met Trp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Ile Ala Cys Ile Tyr Gly Gly Tyr Ser Gly Ser Thr Tyr Tyr 
65                  70                  75                  80  


Ala Ser Trp Ala Lys Gly Arg Phe Thr Ile Ser Lys Thr Ser Ser Thr 
                85                  90                  95      


Thr Val Thr Leu Gln Met Thr Arg Leu Thr Ala Ala Asp Thr Ala Ile 
            100                 105                 110         


Tyr Phe Cys Ala Arg Asp Gly Gly Val Thr His Phe Ser His Phe Asp 
        115                 120                 125             


Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gln Pro Lys 
    130                 135                 140                 


<210>  8
<211>  136
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  14K1 light chain variable region

<400>  8

Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Arg Cys Asp Val Val Met Thr Gln Thr Pro Ala Ser 
            20                  25                  30          


Val Ser Glu Pro Val Gly Gly Thr Val Thr Ile Lys Cys Gln Ala Ser 
        35                  40                  45              


Glu Asp Ile Tyr Arg Leu Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Gly Ala Ser Thr Leu Ala Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Glu Tyr Thr Leu Thr 
                85                  90                  95      


Ile Asn Asp Leu Glu Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Gln Tyr 
            100                 105                 110         


Ile Thr Tyr Gly Ser Asp Val Leu Thr Ala Phe Gly Gly Gly Thr Glu 
        115                 120                 125             


Val Val Val Lys Gly Asp Pro Val 
    130                 135     


<210>  9
<211>  145
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  15H1 heavy chain variable region

<400>  9

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Leu Glu Glu Ser Gly Gly Asp Leu Val Lys Pro 
            20                  25                  30          


Gly Ala Ser Leu Thr Leu Thr Cys Thr Ala Ser Gly Phe Ser Phe Ser 
        35                  40                  45              


Arg Ser Tyr Tyr Val Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Ile Val Cys Ile Tyr Gly Gly Ser Ser Asp Thr Thr Tyr Tyr 
65                  70                  75                  80  


Ala Ser Trp Ala Lys Gly Arg Phe Thr Ile Ser Lys Thr Ser Ser Thr 
                85                  90                  95      


Thr Val Thr Leu Gln Leu Asn Ser Leu Thr Ala Ala Asp Thr Ala Thr 
            100                 105                 110         


Tyr Phe Cys Ala Arg Arg Asp Val Ser Gly Gly Tyr Asp Tyr Gly Met 
        115                 120                 125             


Asp Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gln Pro 
    130                 135                 140                 


Lys 
145 


<210>  10
<211>  136
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  15K3 light chain variable region

<400>  10

Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Arg Cys Ala Tyr Asp Met Thr Gln Thr Pro Ala Ser 
            20                  25                  30          


Val Glu Val Ala Val Gly Gly Thr Val Thr Ile Lys Cys Gln Ala Ser 
        35                  40                  45              


Glu Asp Ile Glu Ser Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
    50                  55                  60                  


Pro Pro Lys Leu Leu Ile Tyr Asp Ala Ser Asp Leu Ala Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Thr Glu Tyr Thr Leu Thr 
                85                  90                  95      


Ile Ser Asp Leu Glu Cys Asp Asp Ala Ala Thr Tyr Tyr Cys Arg Gln 
            100                 105                 110         


Gly Tyr Ser Ser Ser Asn Val Asp Asn Val Phe Gly Gly Gly Thr Glu 
        115                 120                 125             


Val Val Val Lys Gly Asp Pro Val 
    130                 135     


<210>  11
<211>  144
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  24H1 heavy chain variable region

<400>  11

Met Glu Thr Gly Leu Arg Trp Leu Leu Leu Val Ala Val Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Gln Ser Leu Glu Glu Ser Gly Gly Asp Leu Val Lys Pro 
            20                  25                  30          


Gly Ala Ser Leu Thr Leu Thr Cys Ile Gly Ser Gly Phe Asp Phe Ser 
        35                  40                  45              


Ser Asn Ala Ile Trp Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu 
    50                  55                  60                  


Trp Ile Ala Cys Leu Tyr Gly Gly Thr Ser Gly Ser Thr Glu Tyr Ala 
65                  70                  75                  80  


Thr Trp Ala Lys Gly Arg Phe Thr Ile Ser Lys Thr Ser Ser Thr Thr 
                85                  90                  95      


Val Thr Leu Gln Met Thr Ser Leu Thr Asp Ala Asp Thr Ala Thr Tyr 
            100                 105                 110         


Tyr Cys Ala Gly Gly Val Val Thr Trp Ser Tyr Pro Arg Gln Leu Tyr 
        115                 120                 125             


Leu Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gln Pro Lys 
    130                 135                 140                 


<210>  12
<211>  135
<212>  PRT
<213>  Oryctolagus cuniculus


<220>
<221>  MISC_FEATURE
<223>  24K1 light chain variable region

<400>  12

Met Asp Thr Arg Ala Pro Thr Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Pro Gly Ala Arg Cys Ala Asp Val Val Met Thr Gln Thr Pro Ala 
            20                  25                  30          


Ser Val Ser Ala Val Val Gly Gly Thr Val Thr Ile Lys Cys Gln Ala 
        35                  40                  45              


Ser Gln Ser Ile Ser Ser Trp Leu Ser Trp Tyr Gln Gln Lys Leu Gly 
    50                  55                  60                  


Gln Pro Pro Lys Leu Leu Ile Tyr Ser Ala Ser Thr Leu Ala Ser Gly 
65                  70                  75                  80  


Val Pro Ser Arg Phe Lys Gly Ser Gly Ser Gly Ala Asp Tyr Thr Leu 
                85                  90                  95      


Thr Ile Ser Asp Leu Glu Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Gln 
            100                 105                 110         


Ser Asn Thr Ala Val His Thr Tyr Asn Phe Gly Gly Gly Thr Glu Val 
        115                 120                 125             


Val Val Lys Gly Asp Pro Val 
    130                 135 


<210>  13
<211>  15
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  5H2/5K3 epitope 1

<400>  13

Ser Arg Gly Pro Tyr His Pro Ser Glu Cys Cys Phe Thr Tyr Thr 
1               5                   10                  15  


<210>  14
<211>  15
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  5H2/5K3 epitope 2

<400>  14

Tyr Glu Thr Asn Ser Gln Cys Ser Lys Pro Gly Ile Val Phe Ile 
1               5                   10                  15  


<210>  15
<211>  16
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  8H3/8K3 epitope

<400>  15

Tyr Tyr Glu Thr Asn Ser Gln Cys Ser Lys Pro Gly Ile Val Phe Ile 
1               5                   10                  15      


<210>  16
<211>  14
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  9H3/9K2 epitope

<400>  16

Ser Asp Lys Trp Val Gln Asp Tyr Ile Lys Asp Met Lys Glu 
1               5                   10                  


<210>  17
<211>  14
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  14H1/14K1 epitope 1

<400>  17

Cys Cys Phe Thr Tyr Thr Thr Tyr Lys Ile Pro Arg Gln Arg 
1               5                   10                  


<210>  18
<211>  13
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  14H1/14K1 epitope 2

<400>  18

Asn Ser Gln Cys Ser Lys Pro Gly Ile Val Phe Ile Thr 
1               5                   10              


<210>  19
<211>  14
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  15H1/15K3 and 24H1/24K1 epitope sequence

<400>  19

Thr Tyr Lys Ile Pro Arg Gln Arg Ile Met Asp Tyr Tyr Glu 
1               5                   10                  


<210>  20
<211>  93
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  Human CCL14 precursor

<400>  20

Met Lys Ile Ser Val Ala Ala Ile Pro Phe Phe Leu Leu Ile Thr Ile 
1               5                   10                  15      


Ala Leu Gly Thr Lys Thr Glu Ser Ser Ser Arg Gly Pro Tyr His Pro 
            20                  25                  30          


Ser Glu Cys Cys Phe Thr Tyr Thr Thr Tyr Lys Ile Pro Arg Gln Arg 
        35                  40                  45              


Ile Met Asp Tyr Tyr Glu Thr Asn Ser Gln Cys Ser Lys Pro Gly Ile 
    50                  55                  60                  


Val Phe Ile Thr Lys Arg Gly His Ser Val Cys Thr Asn Pro Ser Asp 
65                  70                  75                  80  


Lys Trp Val Gln Asp Tyr Ile Lys Asp Met Lys Glu Asn 
                85                  90              


<210>  21
<211>  17
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  HCC-3 Domain of CCL14

<400>  21

Gln Thr Gly Gly Lys Pro Lys Val Val Lys Ile Gln Leu Lys Leu Val 
1               5                   10                  15      


Gly 
    


