			SEQUENCE LISTING

<110> CODEXIS, INC.
 
<120> ENGINEERED SUCROSE PHOSPHORYLASE VARIANT ENZYMES
 
<130> CX2-192USP1
 
<140> 
<141> 2019-07-02
  
<160> 90
 
<210> 1
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 1
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 2
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 2
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 3
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 3
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 4
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 4
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 5
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 5
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccggcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 6
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 6
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 7
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 7
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gcgagttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 8
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 8
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Glu Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 9
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 9
atgaagaaca aagttcaata tattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 10
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 10
Met Lys Asn Lys Val Gln Tyr Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 11
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 11
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gccttttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 12
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 12
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Leu Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 13
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 13
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtggg 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 14
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 14
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 15
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 15
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag gtgtttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 16
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 16
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Val Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 17
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 17
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttctt gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 18
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 18
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 19
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 19
atgaagaaca aagttcaact gattacctgg gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 20
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 20
Met Lys Asn Lys Val Gln Leu Ile Thr Trp Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 21
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 21
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
gggatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 22
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 22
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gly Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 23
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 23
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacac gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 24
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 24
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Thr Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 25
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 25
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaactt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 26
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 26
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Leu Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 27
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 27
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tgatgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 28
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 28
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Asp 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 29
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 29
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tggttgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 30
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 30
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 31
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 31
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaaggga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 32
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 32
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Gly Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 33
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 33
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtagggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 34
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 34
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Arg Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 35
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 35
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt tttttatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 36
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 36
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Phe Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 37
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 37
atgaagaaca aagttcaaat gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 38
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 38
Met Lys Asn Lys Val Gln Met Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 39
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 39
atgaagaaca aagttcaagt tattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacgt gggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 40
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 40
Met Lys Asn Lys Val Gln Val Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Val Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 41
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 41
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 42
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 42
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 43
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 43
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
ggtatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 44
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 44
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gly Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 45
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 45
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
ggtatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 46
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 46
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gly Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 47
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 47
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 48
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 48
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 49
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 49
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 50
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 50
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 51
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 51
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccctcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 52
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 52
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 53
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 53
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 54
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 54
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 55
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 55
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
ggtatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 56
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 56
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gly Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 57
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 57
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccctcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 58
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 58
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 59
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 59
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccctcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 60
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 60
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 61
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 61
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgac 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 62
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 62
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 63
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 63
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 64
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 64
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 65
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 65
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 66
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 66
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 67
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 67
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
ggtatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 68
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 68
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gly Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 69
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 69
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag gtctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 70
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 70
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Val Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 71
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 71
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccctcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggggattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 72
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 72
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Gly Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 73
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 73
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccgtcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgac 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 74
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 74
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Arg Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 75
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 75
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccctcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
ggtatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 76
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 76
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gly Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 77
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 77
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccctcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
ggtatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 78
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 78
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gly Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 79
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 79
atgaagaaca aagttcaact gattacctac gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcct gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 80
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 80
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Leu Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 81
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 81
atgaagaaca aagttcaact gattacctgg gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac ccctcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gctgcttcat gaccccgaag acctttaaac tggtcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtggc 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 82
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 82
Met Lys Asn Lys Val Gln Leu Ile Thr Trp Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Cys Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Val Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Gly 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 

 
<210> 83
<211> 1500
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 83
atgaagaaca aagttcaact gattacctat gcggatcgtc tgggtgacgg caccctgaag 60
agcatgaccg agaccctgcg taaacacttc gagggtgttt atgaaggcgt gcacatcctg 120
ccgttcttta ccccgttcga tggtgcggac gcgggctttg atccggttga ccacaccaaa 180
gtggacccgc gtctgggtag ctgggacgat gtggcggaac tgagcaccac ccacgatatc 240
atggttgaca ccattgtgaa ccacatgagc tgggagagcg aacagttcca agatgttatg 300
gcgaagggcg aggacagcga atactatccg atgttcctga ccatgagcag catttttccg 360
gatggtgtga ccgaggaaga cctgaccgcg atctatcgtc cgcgtccggg tctgccgttc 420
acccactata actggggtgg caaaacccgt ctggtttgga ccacctttac cccgcagcaa 480
gtggacattg ataccgacag cgagatgggt tggaactacc tgctgagcat cctggatcag 540
ctgagccaaa gccacgttag ccaaattcgt ctggacgcgg tgggttatgg cgcgaaggag 600
aaaaacagca gcctgttcat gaccccgaag acctttaaac tgatcgaacg tattaaggcg 660
gagggtgaaa aacgtggcct ggagaccctg atcgaagttc acagctacta taagaaacag 720
gtggagattg cgagcaaggt ggatcgtgtt tacgactttg cgatcccggg tctgctgctg 780
catgcgctgg aatttggcaa aaccgatgcg ctggcgcaat ggattgacgt tcgtccgaac 840
aacgcggtga acgttctgga tacccacgac ggtatcggcg ttatcgatat tggtagcgat 900
cagatggacc gtagcctggc gggtctggtg ccggatgagg aagttgacgc gctggttgag 960
agcatccacc gtaacagcaa gggtgaaagc caggaagcga ccggcgcggc ggcgagcaac 1020
ctggacctgt accaagttaa ctgcacctac tatgcggcgc tgggtagcga cgatcagaaa 1080
tatattgcgg cgcgtgcggt gcagttcttt atgccgggcg tgccgcaagt ttactatgtg 1140
ggtgcgctgg cgggcagcaa cgatatggac ctgctgaagc gtaccaacag tggtcgtgat 1200
atcaaccgtc actactatag cgcggcggaa gtggcgagcg aggtggaacg tccggtggtt 1260
caggcgctga acgcgctggg ccgtttccgt aacaccctga gcgcgttcga tggtgaattt 1320
agctacagca acgcggacgg cgttctgacc atgacctggg cggatgatgc gacccgtgcg 1380
accctgacct ttgcgccgaa ggcgaacagc aacggtgcga gcgtggcgcg tctggagtgg 1440
accgatgcgg cgggtgaaca tgcgaccgac gatctgatcg cgaacccgcc ggtggttgcg 1500

 
<210> 84
<211> 500
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of  Sucrose phosphorylase from Alloscardovia omnicolens      
 
<400> 84
Met Lys Asn Lys Val Gln Leu Ile Thr Tyr Ala Asp Arg Leu Gly Asp 
1               5                   10                  15      
Gly Thr Leu Lys Ser Met Thr Glu Thr Leu Arg Lys His Phe Glu Gly 
            20                  25                  30          
Val Tyr Glu Gly Val His Ile Leu Pro Phe Phe Thr Pro Phe Asp Gly 
        35                  40                  45              
Ala Asp Ala Gly Phe Asp Pro Val Asp His Thr Lys Val Asp Pro Arg 
    50                  55                  60                  
Leu Gly Ser Trp Asp Asp Val Ala Glu Leu Ser Thr Thr His Asp Ile 
65                  70                  75                  80  
Met Val Asp Thr Ile Val Asn His Met Ser Trp Glu Ser Glu Gln Phe 
                85                  90                  95      
Gln Asp Val Met Ala Lys Gly Glu Asp Ser Glu Tyr Tyr Pro Met Phe 
            100                 105                 110         
Leu Thr Met Ser Ser Ile Phe Pro Asp Gly Val Thr Glu Glu Asp Leu 
        115                 120                 125             
Thr Ala Ile Tyr Arg Pro Arg Pro Gly Leu Pro Phe Thr His Tyr Asn 
    130                 135                 140                 
Trp Gly Gly Lys Thr Arg Leu Val Trp Thr Thr Phe Thr Pro Gln Gln 
145                 150                 155                 160 
Val Asp Ile Asp Thr Asp Ser Glu Met Gly Trp Asn Tyr Leu Leu Ser 
                165                 170                 175     
Ile Leu Asp Gln Leu Ser Gln Ser His Val Ser Gln Ile Arg Leu Asp 
            180                 185                 190         
Ala Val Gly Tyr Gly Ala Lys Glu Lys Asn Ser Ser Leu Phe Met Thr 
        195                 200                 205             
Pro Lys Thr Phe Lys Leu Ile Glu Arg Ile Lys Ala Glu Gly Glu Lys 
    210                 215                 220                 
Arg Gly Leu Glu Thr Leu Ile Glu Val His Ser Tyr Tyr Lys Lys Gln 
225                 230                 235                 240 
Val Glu Ile Ala Ser Lys Val Asp Arg Val Tyr Asp Phe Ala Ile Pro 
                245                 250                 255     
Gly Leu Leu Leu His Ala Leu Glu Phe Gly Lys Thr Asp Ala Leu Ala 
            260                 265                 270         
Gln Trp Ile Asp Val Arg Pro Asn Asn Ala Val Asn Val Leu Asp Thr 
        275                 280                 285             
His Asp Gly Ile Gly Val Ile Asp Ile Gly Ser Asp Gln Met Asp Arg 
    290                 295                 300                 
Ser Leu Ala Gly Leu Val Pro Asp Glu Glu Val Asp Ala Leu Val Glu 
305                 310                 315                 320 
Ser Ile His Arg Asn Ser Lys Gly Glu Ser Gln Glu Ala Thr Gly Ala 
                325                 330                 335     
Ala Ala Ser Asn Leu Asp Leu Tyr Gln Val Asn Cys Thr Tyr Tyr Ala 
            340                 345                 350         
Ala Leu Gly Ser Asp Asp Gln Lys Tyr Ile Ala Ala Arg Ala Val Gln 
        355                 360                 365             
Phe Phe Met Pro Gly Val Pro Gln Val Tyr Tyr Val Gly Ala Leu Ala 
    370                 375                 380                 
Gly Ser Asn Asp Met Asp Leu Leu Lys Arg Thr Asn Ser Gly Arg Asp 
385                 390                 395                 400 
Ile Asn Arg His Tyr Tyr Ser Ala Ala Glu Val Ala Ser Glu Val Glu 
                405                 410                 415     
Arg Pro Val Val Gln Ala Leu Asn Ala Leu Gly Arg Phe Arg Asn Thr 
            420                 425                 430         
Leu Ser Ala Phe Asp Gly Glu Phe Ser Tyr Ser Asn Ala Asp Gly Val 
        435                 440                 445             
Leu Thr Met Thr Trp Ala Asp Asp Ala Thr Arg Ala Thr Leu Thr Phe 
    450                 455                 460                 
Ala Pro Lys Ala Asn Ser Asn Gly Ala Ser Val Ala Arg Leu Glu Trp 
465                 470                 475                 480 
Thr Asp Ala Ala Gly Glu His Ala Thr Asp Asp Leu Ile Ala Asn Pro 
                485                 490                 495     
Pro Val Val Ala                                                 
            500                                                 


<210> 85
<211> 1221
<212> DNA
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 85
atgaaacgtg catttattat ggtgctggac tcattcggca tcggcgctac agaagatgca 60
gaacgctttg gtgacgtcgg ggctgacacc ctgggtcata tcgcagaagc ttgtgccaaa 120
ggcgaagctg ataacggtcg taaaggcccg ctcaatctgc caaatctgac ccgtctgggg 180
ctggcgaaag ctcacgaagg ttctaccggt ttcattccgg cgggaatgga cggcaacgct 240
gaagttatcg gcgcgtacgc atgggcgcac gaaatgtcat ccggtaaaga tagcctgtct 300
ggtcactggg aaattgccgg tgtcccggtt ctgtttgaga tgggatattt caccgatcac 360
gaaaacagct tcccgcaaga gctgctggat aaactggtcg aacgcgctaa tctgccgggt 420
tacctcggta actgccgtgc gtccggtacg gtcattctag atcaactggg cgaagagcac 480
atgaaaaccg gcaagccgat tttctatacc tccgcagcat ccgtgttcca gattgcctgc 540
catgaagaaa ctttcggtct ggataaactc tacgagctgt gcgaaatcgc ccgtgaagag 600
ctgaccaacg gcggctacaa tatcggtcgt gttatcgctc gtccgtttat cggcgacaaa 660
gccggtaact tccaacgtac cggtaaccgt cgtgacctgg ctatagagcc gggggcaccg 720
accgtgctgc agaaactggt tgatgaaaaa cacggccagg tggtttcggc gggtaaaatt 780
gccgacatct acgccaaccg cggtatcacc aaaaaagtga aagcgactgg cctggacgcg 840
ctgtttgacg ccaccatcaa agagatgaaa gaagcgggtg ataacaccat cgtcttcacc 900
tgcttcgttg acttcgactc ttcctggggc caccgtcgcg acgtcgccgg ttatgccgcg 960
ggtctggaac tgttcgaccg ccgtctgccg gagctgatgt ctctgctgcg cgatgacgac 1020
atcctgatcc tcaccgctga ccacggttgc gatccgacct ggaccggtca tgaccacacg 1080
cgtgaacaca ttccggtact ggtatatggc ccgaaagtaa aaccgggctc actgggtcat 1140
cgtgaaacct tcgcggatat cggccagact ctggcaaaat attttggtac ctctgatatg 1200
gaatatggca aagccatgtt c                                           1221

 
<210> 86
<211> 407
<212> PRT
<213> Artificial Sequence
 
<220>
<223> ENGINEERED PHOSPHOPENTOMUTASE VARIANT ENZYMES
 
<400> 86
Met Lys Arg Ala Phe Ile Met Val Leu Asp Ser Phe Gly Ile Gly Ala 
1               5                   10                  15      
Thr Glu Asp Ala Glu Arg Phe Gly Asp Val Gly Ala Asp Thr Leu Gly 
            20                  25                  30          
His Ile Ala Glu Ala Cys Ala Lys Gly Glu Ala Asp Asn Gly Arg Lys 
        35                  40                  45              
Gly Pro Leu Asn Leu Pro Asn Leu Thr Arg Leu Gly Leu Ala Lys Ala 
    50                  55                  60                  
His Glu Gly Ser Thr Gly Phe Ile Pro Ala Gly Met Asp Gly Asn Ala 
65                  70                  75                  80  
Glu Val Ile Gly Ala Tyr Ala Trp Ala His Glu Met Ser Ser Gly Lys 
                85                  90                  95      
Asp Ser Leu Ser Gly His Trp Glu Ile Ala Gly Val Pro Val Leu Phe 
            100                 105                 110         
Glu Met Gly Tyr Phe Thr Asp His Glu Asn Ser Phe Pro Gln Glu Leu 
        115                 120                 125             
Leu Asp Lys Leu Val Glu Arg Ala Asn Leu Pro Gly Tyr Leu Gly Asn 
    130                 135                 140                 
Cys Arg Ala Ser Gly Thr Val Ile Leu Asp Gln Leu Gly Glu Glu His 
145                 150                 155                 160 
Met Lys Thr Gly Lys Pro Ile Phe Tyr Thr Ser Ala Ala Ser Val Phe 
                165                 170                 175     
Gln Ile Ala Cys His Glu Glu Thr Phe Gly Leu Asp Lys Leu Tyr Glu 
            180                 185                 190         
Leu Cys Glu Ile Ala Arg Glu Glu Leu Thr Asn Gly Gly Tyr Asn Ile 
        195                 200                 205             
Gly Arg Val Ile Ala Arg Pro Phe Ile Gly Asp Lys Ala Gly Asn Phe 
    210                 215                 220                 
Gln Arg Thr Gly Asn Arg Arg Asp Leu Ala Ile Glu Pro Gly Ala Pro 
225                 230                 235                 240 
Thr Val Leu Gln Lys Leu Val Asp Glu Lys His Gly Gln Val Val Ser 
                245                 250                 255     
Ala Gly Lys Ile Ala Asp Ile Tyr Ala Asn Arg Gly Ile Thr Lys Lys 
            260                 265                 270         
Val Lys Ala Thr Gly Leu Asp Ala Leu Phe Asp Ala Thr Ile Lys Glu 
        275                 280                 285             
Met Lys Glu Ala Gly Asp Asn Thr Ile Val Phe Thr Cys Phe Val Asp 
    290                 295                 300                 
Phe Asp Ser Ser Trp Gly His Arg Arg Asp Val Ala Gly Tyr Ala Ala 
305                 310                 315                 320 
Gly Leu Glu Leu Phe Asp Arg Arg Leu Pro Glu Leu Met Ser Leu Leu 
                325                 330                 335     
Arg Asp Asp Asp Ile Leu Ile Leu Thr Ala Asp His Gly Cys Asp Pro 
            340                 345                 350         
Thr Trp Thr Gly His Asp His Thr Arg Glu His Ile Pro Val Leu Val 
        355                 360                 365             
Tyr Gly Pro Lys Val Lys Pro Gly Ser Leu Gly His Arg Glu Thr Phe 
    370                 375                 380                 
Ala Asp Ile Gly Gln Thr Leu Ala Lys Tyr Phe Gly Thr Ser Asp Met 
385                 390                 395                 400 
Glu Tyr Gly Lys Ala Met Phe                                     
                405                                             

 
<210> 87
<211> 771
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis
 
<400> 87
atgtgtgatc tgaagaaggc ggcgcagcgg gcgattagcc tgatggatct gaccaccctg 60
aacgacgatg acaccgacca gaaagtgatt gagctgtgcc acaaagcgaa aaccccggcg 120
ggtgacaccg cggcgatcgt gatttacccg cgtttcatcc cgattgcgcg taagaccctg 180
aacgaaatcg gtggcctgga catcaaaatt gtcaccgtta ccaactttcc gcacggtaac 240
gatgacattg cgattgcggt gctggagacc cgtgcggcgg ttgcgtacgg cgcggatgaa 300
gtggacgtgg ttttcccgta tcgtgcgctg atggaaggta acgaaaccgt gggctttgag 360
ctggttaagg cgtgcaaaga ggcgtgcggt gaagatacca ttctgaaggt gatcattgaa 420
agtggcgtac tgaaagaccc ggcgctgatt cgtaaagcga gcgaaattag catcgatgcg 480
ggtgcggact tcattaagac cagcaccggc aaagttgcgg ttaacgcgac cctggaggcg 540
gcggaaatca ttatgaccgt tattagcgag aagaacccga aagtgggttt caagccggcg 600
ggtggcatta aagatgctgc ggcggcggcg gaatttctgg gtgtggcggc gcgtctgctg 660
ggcgatgact gggcgacccc ggcgaccttc cgttttggtg cgaccgacct gctgaccaac 720
ctgctgcaca ccctggaact ggcggacgcg ccgcagggtg cgcaaggcta t          771

 
<210> 88
<211> 257
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Deoxyribose Phosphate Aldolase From Shewanella Halifaxensis
 
<400> 88
Met Cys Asp Leu Lys Lys Ala Ala Gln Arg Ala Ile Ser Leu Met Asp 
1               5                   10                  15      
Leu Thr Thr Leu Asn Asp Asp Asp Thr Asp Gln Lys Val Ile Glu Leu 
            20                  25                  30          
Cys His Lys Ala Lys Thr Pro Ala Gly Asp Thr Ala Ala Ile Val Ile 
        35                  40                  45              
Tyr Pro Arg Phe Ile Pro Ile Ala Arg Lys Thr Leu Asn Glu Ile Gly 
    50                  55                  60                  
Gly Leu Asp Ile Lys Ile Val Thr Val Thr Asn Phe Pro His Gly Asn 
65                  70                  75                  80  
Asp Asp Ile Ala Ile Ala Val Leu Glu Thr Arg Ala Ala Val Ala Tyr 
                85                  90                  95      
Gly Ala Asp Glu Val Asp Val Val Phe Pro Tyr Arg Ala Leu Met Glu 
            100                 105                 110         
Gly Asn Glu Thr Val Gly Phe Glu Leu Val Lys Ala Cys Lys Glu Ala 
        115                 120                 125             
Cys Gly Glu Asp Thr Ile Leu Lys Val Ile Ile Glu Ser Gly Val Leu 
    130                 135                 140                 
Lys Asp Pro Ala Leu Ile Arg Lys Ala Ser Glu Ile Ser Ile Asp Ala 
145                 150                 155                 160 
Gly Ala Asp Phe Ile Lys Thr Ser Thr Gly Lys Val Ala Val Asn Ala 
                165                 170                 175     
Thr Leu Glu Ala Ala Glu Ile Ile Met Thr Val Ile Ser Glu Lys Asn 
            180                 185                 190         
Pro Lys Val Gly Phe Lys Pro Ala Gly Gly Ile Lys Asp Ala Ala Ala 
        195                 200                 205             
Ala Ala Glu Phe Leu Gly Val Ala Ala Arg Leu Leu Gly Asp Asp Trp 
    210                 215                 220                 
Ala Thr Pro Ala Thr Phe Arg Phe Gly Ala Thr Asp Leu Leu Thr Asn 
225                 230                 235                 240 
Leu Leu His Thr Leu Glu Leu Ala Asp Ala Pro Gln Gly Ala Gln Gly 
                245                 250                 255     
Tyr                                                             
                                                                

<210> 89
<211> 717
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 89
atgacgaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca 60
ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg 120
aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta 180
atgggtcacg gtgctggtat cccgagctgc tccatctaca ccaaagaact gatcaccgac 240
ttcggcgtga agaaaattat ccgcgtgggt acttgtggcg cagttctgcc gcacgtaaaa 300
ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt 360
tttaaagacc atgactttgc cgctatcgct gacttcgaca tggtgcgtaa cgcagtagat 420
gcagctaaag cactgggtat tgatccgcgc gtgggtaacc tgcattccgc tgacctgttc 480
tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcattgt gggcgtggaa 540
atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc 600
tgcaccgtat ctgaccacat ccgcactcac gaggctacca ctgcggctga gcgtcagact 660
accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagag    717

 
<210> 90
<211> 239
<212> PRT
<213> Artificial Sequence
 
<220>
<223> Variant of Purine Nucleoside Phosphorylase from E. Coli
 
<400> 90
Met Thr Thr Pro His Ile Asn Ala Glu Met Gly Asp Phe Ala Asp Val 
1               5                   10                  15      
Val Leu Met Pro Gly Asp Pro Leu Arg Ala Lys Tyr Ile Ala Glu Thr 
            20                  25                  30          
Phe Leu Glu Asp Ala Arg Glu Val Asn Asn Val Arg Gly Met Leu Gly 
        35                  40                  45              
Phe Thr Gly Thr Tyr Lys Gly Arg Lys Ile Ser Val Met Gly His Gly 
    50                  55                  60                  
Ala Gly Ile Pro Ser Cys Ser Ile Tyr Thr Lys Glu Leu Ile Thr Asp 
65                  70                  75                  80  
Phe Gly Val Lys Lys Ile Ile Arg Val Gly Thr Cys Gly Ala Val Leu 
                85                  90                  95      
Pro His Val Lys Leu Arg Asp Val Val Ile Gly Met Gly Ala Cys Thr 
            100                 105                 110         
Asp Ser Lys Val Asn Arg Ile Arg Phe Lys Asp His Asp Phe Ala Ala 
        115                 120                 125             
Ile Ala Asp Phe Asp Met Val Arg Asn Ala Val Asp Ala Ala Lys Ala 
    130                 135                 140                 
Leu Gly Ile Asp Pro Arg Val Gly Asn Leu His Ser Ala Asp Leu Phe 
145                 150                 155                 160 
Tyr Ser Pro Asp Gly Glu Met Phe Asp Val Met Glu Lys Tyr Gly Ile 
                165                 170                 175     
Val Gly Val Glu Met Glu Ala Ala Gly Ile Tyr Gly Val Ala Ala Glu 
            180                 185                 190         
Phe Gly Ala Lys Ala Leu Thr Ile Cys Thr Val Ser Asp His Ile Arg 
        195                 200                 205             
Thr His Glu Ala Thr Thr Ala Ala Glu Arg Gln Thr Thr Phe Asn Asp 
    210                 215                 220                 
Met Ile Lys Ile Ala Leu Glu Ser Val Leu Leu Gly Asp Lys Glu     
225                 230                 235                     

 