﻿               SEQUENCE LISTING

<110> Max-Delbrueck-Centrum fuer Molekulare Medizin
      Charité - Universitaetsmedizin Berlin

<120> CD5 specific T cell receptor cell or gene therapy

<130> 11915 P 6581 EP

<150> EP19183694.9
<151> 2019-07-01

<160> 45

<170> BiSSAP 1.3.6

<210> 1
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> epitope from CD5

<400> 1
Tyr Leu Lys Asp Gly Trp His Met Val 
1               5                   

<210> 2
<211> 7
<212> PRT
<213> Homo sapiens


<220> 
<223> T-20109 + T-20332 alpha chain CDR1

<400> 2
Thr Ser Asp Pro Ser Tyr Gly 
1               5           

<210> 3
<211> 8
<212> PRT
<213> Homo sapiens


<220> 
<223> T-20109 + T-20332 alpha chain CDR2

<400> 3
Gln Gly Ser Tyr Asp Gln Gln Asn 
1               5               

<210> 4
<211> 15
<212> PRT
<213> Homo sapiens


<220> 
<223> T-20109 + T-20332 alpha chain CDR3

<400> 4
Ala Met Arg Glu Glu Tyr Ser Gly Ala Gly Ser Tyr Gln Leu Thr 
1               5                   10                  15  

<210> 5
<211> 5
<212> PRT
<213> Homo sapiens


<220> 
<223> T-20109 + T-20332 beta chain CDR1

<400> 5
Ser Asn His Leu Tyr 
1               5   

<210> 6
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> T-20109 + T-20332 beta chain CDR2

<400> 6
Phe Tyr Asn Asn Glu Ile 
1               5       

<210> 7
<211> 11
<212> PRT
<213> Artificial Sequence


<220> 
<223> beta chain CDR3 consensus sequence

<220> 
<221> VARIANT
<222> 4
<223> Xaa can be any naturally occurring amino acid

<400> 7
Ala Ser Ser Xaa Gly Pro Tyr Asn Glu Gln Phe 
1               5                   10      

<210> 8
<211> 11
<212> PRT
<213> Homo sapiens


<400> 8
Ala Ser Ser Gln Gly Pro Tyr Asn Glu Gln Phe 
1               5                   10      

<210> 9
<211> 11
<212> PRT
<213> Homo sapiens


<220> 
<223> T-20332 beta chain CDR3

<400> 9
Ala Ser Ser Arg Gly Pro Tyr Asn Glu Gln Phe 
1               5                   10      

<210> 10
<211> 138
<212> PRT
<213> Homo sapiens


<220> 
<223> variable region T-20109 alpha chain (aa)

<400> 10
Met Ser Leu Ser Ser Leu Leu Lys Val Val Thr Ala Ser Leu Trp Leu 
1               5                   10                  15      
Gly Pro Gly Ile Ala Gln Lys Ile Thr Gln Thr Gln Pro Gly Met Phe 
            20                  25                  30          
Val Gln Glu Lys Glu Ala Val Thr Leu Asp Cys Thr Tyr Asp Thr Ser 
        35                  40                  45              
Asp Pro Ser Tyr Gly Leu Phe Trp Tyr Lys Gln Pro Ser Ser Gly Glu 
    50                  55                  60                  
Met Ile Phe Leu Ile Tyr Gln Gly Ser Tyr Asp Gln Gln Asn Ala Thr 
65                  70                  75                  80  
Glu Gly Arg Tyr Ser Leu Asn Phe Gln Lys Ala Arg Lys Ser Ala Asn 
                85                  90                  95      
Leu Val Ile Ser Ala Ser Gln Leu Gly Asp Ser Ala Met Tyr Phe Cys 
            100                 105                 110         
Ala Met Arg Glu Glu Tyr Ser Gly Ala Gly Ser Tyr Gln Leu Thr Phe 
        115                 120                 125             
Gly Lys Gly Thr Lys Leu Ser Val Ile Pro 
    130                 135             

<210> 11
<211> 132
<212> PRT
<213> Homo sapiens


<220> 
<223> variable region T-20109 beta chain(aa)

<400> 11
Met Asp Thr Trp Leu Val Cys Trp Ala Ile Phe Ser Leu Leu Lys Ala 
1               5                   10                  15      
Gly Leu Thr Glu Pro Glu Val Thr Gln Thr Pro Ser His Gln Val Thr 
            20                  25                  30          
Gln Met Gly Gln Glu Val Ile Leu Arg Cys Val Pro Ile Ser Asn His 
        35                  40                  45              
Leu Tyr Phe Tyr Trp Tyr Arg Gln Ile Leu Gly Gln Lys Val Glu Phe 
    50                  55                  60                  
Leu Val Ser Phe Tyr Asn Asn Glu Ile Ser Glu Lys Ser Glu Ile Phe 
65                  70                  75                  80  
Asp Asp Gln Phe Ser Val Glu Arg Pro Asp Gly Ser Asn Phe Thr Leu 
                85                  90                  95      
Lys Ile Arg Ser Thr Lys Leu Glu Asp Ser Ala Met Tyr Phe Cys Ala 
            100                 105                 110         
Ser Ser Gln Gly Pro Tyr Asn Glu Gln Phe Phe Gly Pro Gly Thr Arg 
        115                 120                 125             
Leu Thr Val Leu 
    130         

<210> 12
<211> 138
<212> PRT
<213> Homo sapiens


<220> 
<223> variable region T-20332 alpha chain (aa)

<400> 12
Met Ser Leu Ser Ser Leu Leu Lys Val Val Thr Ala Ser Leu Trp Leu 
1               5                   10                  15      
Gly Pro Gly Ile Ala Gln Lys Ile Thr Gln Thr Gln Pro Gly Met Phe 
            20                  25                  30          
Val Gln Glu Lys Glu Ala Val Thr Leu Asp Cys Thr Tyr Asp Thr Ser 
        35                  40                  45              
Asp Pro Ser Tyr Gly Leu Phe Trp Tyr Lys Gln Pro Ser Ser Gly Glu 
    50                  55                  60                  
Met Ile Phe Leu Ile Tyr Gln Gly Ser Tyr Asp Gln Gln Asn Ala Thr 
65                  70                  75                  80  
Glu Gly Arg Tyr Ser Leu Asn Phe Gln Lys Ala Arg Lys Ser Ala Asn 
                85                  90                  95      
Leu Val Ile Ser Ala Ser Gln Leu Gly Asp Ser Ala Met Tyr Phe Cys 
            100                 105                 110         
Ala Met Arg Glu Glu Tyr Ser Gly Ala Gly Ser Tyr Gln Leu Thr Phe 
        115                 120                 125             
Gly Lys Gly Thr Lys Leu Ser Val Ile Pro 
    130                 135             

<210> 13
<211> 132
<212> PRT
<213> Homo sapiens


<220> 
<223> variable region T-20332 beta chain (aa)

<400> 13
Met Asp Thr Trp Leu Val Cys Trp Ala Ile Phe Ser Leu Leu Lys Ala 
1               5                   10                  15      
Gly Leu Thr Glu Pro Glu Val Thr Gln Thr Pro Ser His Gln Val Thr 
            20                  25                  30          
Gln Met Gly Gln Glu Val Ile Leu Arg Cys Val Pro Ile Ser Asn His 
        35                  40                  45              
Leu Tyr Phe Tyr Trp Tyr Arg Gln Ile Leu Gly Gln Lys Val Glu Phe 
    50                  55                  60                  
Leu Val Ser Phe Tyr Asn Asn Glu Ile Ser Glu Lys Ser Glu Ile Phe 
65                  70                  75                  80  
Asp Asp Gln Phe Ser Val Glu Arg Pro Asp Gly Ser Asn Phe Thr Leu 
                85                  90                  95      
Lys Ile Arg Ser Thr Lys Leu Glu Asp Ser Ala Met Tyr Phe Cys Ala 
            100                 105                 110         
Ser Ser Arg Gly Pro Tyr Asn Glu Gln Phe Phe Gly Pro Gly Thr Arg 
        115                 120                 125             
Leu Thr Val Leu 
    130         

<210> 14
<211> 414
<212> DNA
<213> Artificial Sequence


<220> 
<223> variable region T-20109 alpha chain (na) (codon optimized)

<400> 14
atgtctctgt cctctctgct gaaagtggtc accgccagcc tgtggctcgg acctggaatt      60

gcccagaaga tcacccagac acagcccggc atgttcgtgc aagagaaaga agccgtgaca     120

ctggactgca cctacgacac cagcgatcct agctacggcc tgttctggta caagcagcct     180

agcagcggcg agatgatctt cctgatctac cagggcagct acgaccagca gaacgccaca     240

gagggcagat acagcctgaa cttccagaag gcccggaagt ccgccaacct ggtcatttct     300

gctagccagc tgggcgactc cgccatgtat ttctgtgcca tgcgggaaga gtacagcgga     360

gccggaagct accagctgac atttggcaag ggcacaaagc tgagcgtgat cccc           414


<210> 15
<211> 396
<212> DNA
<213> Artificial Sequence


<220> 
<223> variable region T-20109 beta chain (na) (codon optimized)

<400> 15
atggatactt ggcttgtgtg ctgggccatc ttcagcctgc tgaaggccgg actgacagag      60

cccgaagtga cacagacacc cagccaccaa gtgacccaga tgggccaaga agtgatcctg     120

cgctgcgtgc ccatcagcaa ccacctgtac ttctactggt acagacagat cctgggccag     180

aaagtcgagt tcctggtgtc cttctacaac aacgagatca gcgagaagtc cgagatcttc     240

gacgaccagt tcagcgtgga aagacccgac ggcagcaact tcaccctgaa gatcagaagc     300

accaagctcg aggacagcgc catgtacttt tgcgcctctt ctcagggccc ctacaacgag     360

cagtttttcg gccctggcac cagactgacc gtgctg                               396


<210> 16
<211> 414
<212> DNA
<213> Homo sapiens


<220> 
<223> variable region T-20332 alpha chain (na)

<400> 16
atgtctctgt cctctctgct gaaagtggtc accgccagcc tgtggctcgg acctggaatt      60

gcccagaaga tcacccagac acagcccggc atgttcgtgc aagagaaaga agccgtgaca     120

ctggactgca cctacgacac cagcgatcct agctacggcc tgttctggta caagcagcct     180

agcagcggcg agatgatctt cctgatctac cagggcagct acgaccagca gaacgccaca     240

gagggcagat acagcctgaa cttccagaag gcccggaagt ccgccaacct ggtcatttct     300

gctagccagc tgggcgactc cgccatgtat ttctgtgcca tgcgggaaga gtacagcgga     360

gccggaagct accagctgac atttggcaag ggcacaaagc tgagcgtgat cccc           414


<210> 17
<211> 396
<212> DNA
<213> Homo sapiens


<220> 
<223> variable region T-20332 beta chain (na)

<400> 17
atggatactt ggcttgtgtg ctgggccatc ttcagcctgc tgaaggccgg actgacagag      60

cccgaagtga cacagacacc cagccaccaa gtgacccaga tgggccaaga agtgatcctg     120

cgctgcgtgc ccatcagcaa ccacctgtac ttctactggt acagacagat cctgggccag     180

aaagtcgagt tcctggtgtc cttctacaac aacgagatca gcgagaagtc cgagatcttc     240

gacgaccagt tcagcgtgga aagacccgac ggcagcaact tcaccctgaa gatcagaagc     300

accaagctcg aggacagcgc catgtacttt tgcgcctctt ctagaggccc ctacaacgag     360

cagtttttcg gccctggcac cagactgacc gtgctg                               396


<210> 18
<211> 136
<212> PRT
<213> Mus musculus


<220> 
<223> murine constant region (alpha chain)

<400> 18
Ile Gln Asn Pro Glu Pro Ala Val Tyr Gln Leu Lys Asp Pro Arg Ser 
1               5                   10                  15      
Gln Asp Ser Thr Leu Cys Leu Phe Thr Asp Phe Asp Ser Gln Ile Asn 
            20                  25                  30          
Val Pro Lys Thr Met Glu Ser Gly Thr Phe Ile Thr Asp Lys Thr Val 
        35                  40                  45              
Leu Asp Met Lys Ala Met Asp Ser Lys Ser Asn Gly Ala Ile Ala Trp 
    50                  55                  60                  
Ser Asn Gln Thr Ser Phe Thr Cys Gln Asp Ile Phe Lys Glu Thr Asn 
65                  70                  75                  80  
Ala Thr Tyr Pro Ser Ser Asp Val Pro Cys Asp Ala Thr Leu Thr Glu 
                85                  90                  95      
Lys Ser Phe Glu Thr Asp Met Asn Leu Asn Phe Gln Asn Leu Ser Val 
            100                 105                 110         
Met Gly Leu Arg Ile Leu Leu Leu Lys Val Ala Gly Phe Asn Leu Leu 
        115                 120                 125             
Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135     

<210> 19
<211> 140
<212> PRT
<213> Mus musculus


<220> 
<223> minimally murine constant region (alpha chain)

<400> 19
Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
1               5                   10                  15      
Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
            20                  25                  30          
Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
        35                  40                  45              
Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
    50                  55                  60                  
Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
65                  70                  75                  80  
Ile Pro Glu Asp Thr Phe Phe Pro Ser Ser Asp Val Pro Cys Asp Val 
                85                  90                  95      
Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
            100                 105                 110         
Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
        115                 120                 125             
Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140 

<210> 20
<211> 140
<212> PRT
<213> Homo sapiens


<220> 
<223> human constant region (alpha chain)

<400> 20
Ile Gln Asn Pro Asp Pro Ala Val Tyr Gln Leu Arg Asp Ser Lys Ser 
1               5                   10                  15      
Ser Asp Lys Ser Val Cys Leu Phe Thr Asp Phe Asp Ser Gln Thr Asn 
            20                  25                  30          
Val Ser Gln Ser Lys Asp Ser Asp Val Tyr Ile Thr Asp Lys Thr Val 
        35                  40                  45              
Leu Asp Met Arg Ser Met Asp Phe Lys Ser Asn Ser Ala Val Ala Trp 
    50                  55                  60                  
Ser Asn Lys Ser Asp Phe Ala Cys Ala Asn Ala Phe Asn Asn Ser Ile 
65                  70                  75                  80  
Ile Pro Glu Asp Thr Phe Phe Pro Ser Pro Glu Ser Ser Cys Asp Val 
                85                  90                  95      
Lys Leu Val Glu Lys Ser Phe Glu Thr Asp Thr Asn Leu Asn Phe Gln 
            100                 105                 110         
Asn Leu Ser Val Ile Gly Phe Arg Ile Leu Leu Leu Lys Val Ala Gly 
        115                 120                 125             
Phe Asn Leu Leu Met Thr Leu Arg Leu Trp Ser Ser 
    130                 135                 140 

<210> 21
<211> 173
<212> PRT
<213> Mus musculus


<220> 
<223> murine constant region (beta)

<400> 21
Glu Asp Leu Arg Asn Val Thr Pro Pro Lys Val Ser Leu Phe Glu Pro 
1               5                   10                  15      
Ser Lys Ala Glu Ile Ala Asn Lys Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          
Ala Arg Gly Phe Phe Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Ala Tyr Lys 
    50                  55                  60                  
Glu Ser Asn Tyr Ser Tyr Cys Leu Ser Ser Arg Leu Arg Val Ser Ala 
65                  70                  75                  80  
Thr Phe Trp His Asn Pro Arg Asn His Phe Arg Cys Gln Val Gln Phe 
                85                  90                  95      
His Gly Leu Ser Glu Glu Asp Lys Trp Pro Glu Gly Ser Pro Lys Pro 
            100                 105                 110         
Val Thr Gln Asn Ile Ser Ala Glu Ala Trp Gly Arg Ala Asp Cys Gly 
        115                 120                 125             
Ile Thr Ser Ala Ser Tyr His Gln Gly Val Leu Ser Ala Thr Ile Leu 
    130                 135                 140                 
Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala Val Leu Val Ser 
145                 150                 155                 160 
Gly Leu Val Leu Met Ala Met Val Lys Lys Lys Asn Ser 
                165                 170             

<210> 22
<211> 179
<212> PRT
<213> Artificial Sequence


<220> 
<223> minimally murine constant region (beta chain)

<400> 22
Glu Asp Leu Lys Asn Val Phe Pro Pro Lys Val Ala Val Phe Glu Pro 
1               5                   10                  15      
Ser Lys Ala Glu Ile Ala His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          
Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  
Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  
Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      
Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         
Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             
Ala Asp Cys Gly Ile Thr Ser Ala Ser Tyr His Gln Gly Val Leu Ser 
    130                 135                 140                 
Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 
Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     
Ser Arg Gly 
            

<210> 23
<211> 179
<212> PRT
<213> Homo sapiens


<220> 
<223> human constant region (beta chain)

<400> 23
Glu Asp Leu Lys Asn Val Phe Pro Pro Lys Val Ala Val Phe Glu Pro 
1               5                   10                  15      
Ser Glu Ala Glu Ile Ser His Thr Gln Lys Ala Thr Leu Val Cys Leu 
            20                  25                  30          
Ala Thr Gly Phe Tyr Pro Asp His Val Glu Leu Ser Trp Trp Val Asn 
        35                  40                  45              
Gly Lys Glu Val His Ser Gly Val Ser Thr Asp Pro Gln Pro Leu Lys 
    50                  55                  60                  
Glu Gln Pro Ala Leu Asn Asp Ser Arg Tyr Cys Leu Ser Ser Arg Leu 
65                  70                  75                  80  
Arg Val Ser Ala Thr Phe Trp Gln Asn Pro Arg Asn His Phe Arg Cys 
                85                  90                  95      
Gln Val Gln Phe Tyr Gly Leu Ser Glu Asn Asp Glu Trp Thr Gln Asp 
            100                 105                 110         
Arg Ala Lys Pro Val Thr Gln Ile Val Ser Ala Glu Ala Trp Gly Arg 
        115                 120                 125             
Ala Asp Cys Gly Phe Thr Ser Glu Ser Tyr Gln Gln Gly Val Leu Ser 
    130                 135                 140                 
Ala Thr Ile Leu Tyr Glu Ile Leu Leu Gly Lys Ala Thr Leu Tyr Ala 
145                 150                 155                 160 
Val Leu Val Ser Ala Leu Val Leu Met Ala Met Val Lys Arg Lys Asp 
                165                 170                 175     
Ser Arg Gly 
            

<210> 24
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer for TCRA

<400> 24
cggccacttt caggaggagg attcggacc                                      29


<210> 25
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> reverse primer for TCRB

<400> 25
ccgtagaact ggacttgaca gcggaagtgg                                     30


<210> 26
<211> 30
<212> PRT
<213> Homo sapiens


<220> 
<223> sequence from human CD5 (Fig. 1a)

<400> 26
Ser Asn Ser Lys Cys Gln Gly Gln Leu Glu Val Tyr Leu Lys Asp Gly 
1               5                   10                  15      
Trp His Met Val Cys Ser Gln Ser Trp Gly Arg Ser Ser Lys 
            20                  25                  30  

<210> 27
<211> 30
<212> PRT
<213> Mus musculus


<220> 
<223> sequence from mouse CD5 (Fig. 1b)

<400> 27
Ser Asn Ser Lys Cys Gln Gly Gln Val Glu Ile Gln Met Glu Asn Lys 
1               5                   10                  15      
Trp Lys Thr Val Cys Ser Ser Ser Trp Arg Leu Ser Gln Asp 
            20                  25                  30  

<210> 28
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> crRNA-spacer A2-1

<400> 28
catggagtga gagcccgccc                                                20


<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> crRNA-spacer A2-2

<400> 29
cctctggacg gtgtgagaac                                                20


<210> 30
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> crRNA-spacer A2-3

<400> 30
cggggagaca cggaaagtg                                                 19


<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> crRNA-spacer A2-4

<400> 31
aggcgtactg gtggtacccg                                                20


<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> crRNA-spacer A2-5

<400> 32
tgccgtcgta ggcgtactgg                                                20


<210> 33
<211> 9
<212> PRT
<213> Homo sapiens


<220> 
<223> epitope from CD5

<400> 33
Ser Ile Cys Glu Gly Thr Val Glu Val 
1               5                   

<210> 34
<211> 7
<212> PRT
<213> Homo sapiens


<220> 
<223> T-7378 alpha chain CDR1

<400> 34
Thr Ser Glu Ser Asp Tyr Tyr 
1               5           

<210> 35
<211> 8
<212> PRT
<213> Homo sapiens


<220> 
<223> T-7378 alpha chain CDR2

<400> 35
Gln Glu Ala Tyr Lys Gln Gln Asn 
1               5               

<210> 36
<211> 12
<212> PRT
<213> Homo sapiens


<220> 
<223> T-7378 alpha chain CDR3

<400> 36
Ala Tyr Arg Gly Thr Ser Gly Thr Tyr Lys Tyr Ile 
1               5                   10          

<210> 37
<211> 5
<212> PRT
<213> Homo sapiens


<220> 
<223> T-7378 beta chain CDR1

<400> 37
Met Gly His Arg Ala 
1               5   

<210> 38
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> T-7378 beta chain CDR2

<400> 38
Tyr Ser Tyr Glu Lys Leu 
1               5       

<210> 39
<211> 13
<212> PRT
<213> Homo sapiens


<220> 
<223> T-7378 beta chain CDR3

<400> 39
Ala Ser Ser Arg Gly Leu Ala Gly Pro Tyr Glu Gln Tyr 
1               5                   10              

<210> 40
<211> 136
<212> PRT
<213> Homo sapiens


<220> 
<223> variable region T-7378 alpha chain (aa)

<400> 40
Met Ala Cys Pro Gly Phe Leu Trp Ala Leu Val Ile Ser Thr Cys Leu 
1               5                   10                  15      
Glu Phe Ser Met Ala Gln Thr Val Thr Gln Ser Gln Pro Glu Met Ser 
            20                  25                  30          
Val Gln Glu Ala Glu Thr Val Thr Leu Ser Cys Thr Tyr Asp Thr Ser 
        35                  40                  45              
Glu Ser Asp Tyr Tyr Leu Phe Trp Tyr Lys Gln Pro Pro Ser Arg Gln 
    50                  55                  60                  
Met Ile Leu Val Ile Arg Gln Glu Ala Tyr Lys Gln Gln Asn Ala Thr 
65                  70                  75                  80  
Glu Asn Arg Phe Ser Val Asn Phe Gln Lys Ala Ala Lys Ser Phe Ser 
                85                  90                  95      
Leu Lys Ile Ser Asp Ser Gln Leu Gly Asp Ala Ala Met Tyr Phe Cys 
            100                 105                 110         
Ala Tyr Arg Gly Thr Ser Gly Thr Tyr Lys Tyr Ile Phe Gly Thr Gly 
        115                 120                 125             
Thr Met Leu Lys Val Leu Ala Asn 
    130                 135     

<210> 41
<211> 133
<212> PRT
<213> Homo sapiens


<220> 
<223> variable region T-7378 beta chain (aa)

<400> 41
Met Gly Cys Arg Leu Leu Cys Cys Ala Val Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      
Val Pro Ile Asp Thr Glu Val Thr Gln Thr Pro Lys His Leu Val Met 
            20                  25                  30          
Gly Met Thr Asn Lys Lys Ser Leu Lys Cys Glu Gln His Met Gly His 
        35                  40                  45              
Arg Ala Met Tyr Trp Tyr Lys Gln Lys Ala Lys Lys Pro Pro Glu Leu 
    50                  55                  60                  
Met Phe Val Tyr Ser Tyr Glu Lys Leu Ser Ile Asn Glu Ser Val Pro 
65                  70                  75                  80  
Ser Arg Phe Ser Pro Glu Cys Pro Asn Ser Ser Leu Leu Asn Leu His 
                85                  90                  95      
Leu His Ala Leu Gln Pro Glu Asp Ser Ala Leu Tyr Leu Cys Ala Ser 
            100                 105                 110         
Ser Arg Gly Leu Ala Gly Pro Tyr Glu Gln Tyr Phe Gly Pro Gly Thr 
        115                 120                 125             
Arg Leu Thr Val Thr 
    130             

<210> 42
<211> 408
<212> DNA
<213> Homo sapiens


<220> 
<223> variable region T-7378 alpha chain (na)

<400> 42
atggcatgcc ctggcttcct gtgggcactt gtgatctcca cctgtcttga atttagcatg      60

gctcagacag tcactcagtc tcaaccagag atgtctgtgc aggaggcaga gaccgtgacc     120

ctgagctgca catatgacac cagtgagagt gattattatt tattctggta caagcagcct     180

cccagcaggc agatgattct cgttattcgc caagaagctt ataagcaaca gaatgcaaca     240

gagaatcgtt tctctgtgaa cttccagaaa gcagccaaat ccttcagtct caagatctca     300

gactcacagc tgggggatgc cgcgatgtat ttctgtgctt ataggggtac ctcaggaacc     360

tacaaataca tctttggaac aggcaccatg ctgaaggttt tagcaaat                  408


<210> 43
<211> 399
<212> DNA
<213> Homo sapiens


<220> 
<223> variable region T-7378 beta chain (na)

<400> 43
atgggctgca ggctgctctg ctgtgcggtt ctctgtctcc tgggagcagt tcccatagac      60

actgaagtta cccagacacc aaaacacctg gtcatgggaa tgacaaataa gaagtctttg     120

aaatgtgaac aacatatggg gcacagggct atgtattggt acaagcagaa agctaagaag     180

ccaccggagc tcatgtttgt ctacagctat gagaaactct ctataaatga aagtgtgcca     240

agtcgcttct cacctgaatg ccccaacagc tctctcttaa accttcacct acacgccctg     300

cagccagaag actcagccct gtatctctgc gccagcagcc gcggactagc gggaccctac     360

gagcagtact tcgggccggg caccaggctc acggtcaca                            399


<210> 44
<211> 408
<212> DNA
<213> Artificial Sequence


<220> 
<223> variable region T-7378 alpha chain (na) (codon optimized)

<400> 44
atggcctgtc caggatttct gtgggccctc gtgatcagca cctgtctcga gtttagcatg      60

gcccagaccg tgacacagtc ccagcctgag atgtctgtgc aagaggccga gacagtgacc     120

ctgagctgca cctacgatac cagcgagagc gactactacc tgttctggta taagcagcct     180

cctagccggc agatgatcct ggtcatcaga caagaggctt acaagcagca gaacgccaca     240

gagaaccgct tcagcgtgaa cttccagaag gccgccaaga gctttagcct gaaaatcagc     300

gacagccagc tgggcgacgc cgccatgtat ttctgtgcct acagaggcac cagcggcacc     360

tacaagtaca tctttggcac cggcacaatg ctgaaggtgc tggccaat                  408


<210> 45
<211> 399
<212> DNA
<213> Artificial Sequence


<220> 
<223> variable region T-7378 beta chain (na) (codon optimized)

<400> 45
atgggatgta gactgctgtg ttgtgccgtg ctgtgtctgc tgggagccgt gcctatcgac      60

accgaagtga cccagacacc taagcacctg gtcatgggca tgacaaacaa gaaaagcctg     120

aagtgcgagc agcacatggg ccacagagcc atgtactggt acaagcagaa ggccaagaaa     180

cctcctgagc tgatgttcgt gtacagctac gagaagctga gcatcaacga gagcgtgccc     240

agcagattca gccctgagtg ccctaatagc agcctgctga acctgcatct gcacgccctg     300

cagcctgaag atagcgccct gtatctgtgc gccagctcta gaggactggc cggaccttac     360

gagcagtact ttggccctgg caccagactg accgtgacc                            399


