                               SEQUENCE LISTING

<110> ROCHE SEQUENCING SOLUTIONS, INC.
 
<120> PEPTIDE LIBRARIES HAVING ENHANCED SUBSEQUENCE DIVERSITY AND 
      METHODS FOR USE THEREOF

<130> 124009-0220

<140> PCT/US2020/040007
<141> 2020-06-26

<150> 62/867,765
<151> 2019-06-27

<150> 62/867,666
<151> 2019-06-27

<160> 80    

<170> PatentIn version 3.5

<210> 1
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 1
Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210> 2
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      6xHis tag"

<400> 2
His His His His His His 
1               5       


<210> 3
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 3
Met Met Met Met Met 
1               5   


<210> 4
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 4
Ala Met Met Met Met 
1               5   


<210> 5
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 5
Pro Met Met Met Met 
1               5   


<210> 6
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 6
Val Met Met Met Met 
1               5   


<210> 7
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 7
Gln Met Met Met Met 
1               5   


<210> 8
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 8
Met Ala Met Met Met 
1               5   


<210> 9
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 9
Met Gln Met Met Met 
1               5   


<210> 10
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 10
Met Pro Met Met Met 
1               5   


<210> 11
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 11
Met Asn Met Met Met 
1               5   


<210> 12
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 12
Pro Ala Met Met Met 
1               5   


<210> 13
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 13
Pro Phe Met Met Met 
1               5   


<210> 14
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 14
Pro Val Met Met Met 
1               5   


<210> 15
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 15
Pro Glu Met Met Met 
1               5   


<210> 16
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 16
Val Ala Met Met Met 
1               5   


<210> 17
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 17
Val Phe Met Met Met 
1               5   


<210> 18
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 18
Val Val Met Met Met 
1               5   


<210> 19
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 19
Val Glu Met Met Met 
1               5   


<210> 20
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 20
Ala Ala Met Met Met 
1               5   


<210> 21
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 21
Ala Phe Met Met Met 
1               5   


<210> 22
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 22
Ala Val Met Met Met 
1               5   


<210> 23
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 23
Ala Glu Met Met Met 
1               5   


<210> 24
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 24
Met Met Met Met 
1               


<210> 25
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"


<220>
<221> MOD_RES
<222> (1)..(1)
<223> Any of the 20 natural amino acids 

<400> 25
Xaa Met Met Met Met Met 
1               5       


<210> 26
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Any of the 20 natural amino acids 

<400> 26
Met Xaa Met Met Met Met 
1               5       


<210> 27
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any of the 20 natural amino acids 

<400> 27
Met Met Xaa Met Met Met 
1               5       


<210> 28
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"


<220>
<221> MOD_RES
<222> (4)..(4)
<223> Any of the 20 natural amino acids 

<400> 28
Met Met Met Xaa Met Met 
1               5       


<210> 29
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"


<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any of the 20 natural amino acids 

<400> 29
Met Met Met Met Xaa Met 
1               5       


<210> 30
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"


<220>
<221> MOD_RES
<222> (6)..(6)
<223> Any of the 20 natural amino acids 

<400> 30
Met Met Met Met Met Xaa 
1               5       


<210> 31
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 31
Asn Asp Tyr Gln Tyr Lys Gly Gln Gln Cys Trp Asp Pro Arg Tyr 
1               5                   10                  15  


<210> 32
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 32
His Asn Trp Ala Ala Gln Cys Thr Asn Pro Asp Gln Ala Lys Leu 
1               5                   10                  15  


<210> 33
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 33
Trp Ser Leu Gly Gln Cys Val Ala Asp Gln Pro Asn Gln Thr Trp 
1               5                   10                  15  


<210> 34
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 34
Ser Ile Ile Gly Gln Cys Tyr Ser Lys Asp Pro Ser Gln Ala Thr 
1               5                   10                  15  


<210> 35
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 35
Gln Asn Phe Tyr Gly Glu Thr Arg Phe Thr Pro Ile Ser Ser Trp 
1               5                   10                  15  


<210> 36
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 36
Leu Ser Tyr Asn Leu Phe Tyr Gly Lys Cys Trp Ala Pro Trp Phe 
1               5                   10                  15  


<210> 37
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 37
Cys Gln Asn Pro Ala Tyr Gln Lys Lys Tyr Asn Trp Glu Pro Val 
1               5                   10                  15  


<210> 38
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 38
Ile Trp Leu Gly Glu Cys Cys Lys Asn Asn Tyr Ser Arg Ala Arg 
1               5                   10                  15  


<210> 39
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 39
Val Ile Val Gly Gln Cys Leu Lys Lys Tyr Asn Asp Thr Arg Val 
1               5                   10                  15  


<210> 40
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 40
Lys Pro Ile Tyr Leu Gly Gln Cys Cys Asn Thr Ser His Ala Arg 
1               5                   10                  15  


<210> 41
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 41
Arg Ser Asn Asn Asp Ile Cys Phe Trp Thr Gly Gln Gln Cys Cys 
1               5                   10                  15  


<210> 42
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 42
Tyr Val Tyr Gly Thr Cys Trp Lys Asp Tyr Thr Asp Thr Asp Lys 
1               5                   10                  15  


<210> 43
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 43
Gln Ile Gly Gln Cys Leu Lys Asn Val Gly Tyr Arg Glu Val Pro 
1               5                   10                  15  


<210> 44
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 44
Phe Gln Val Gly Gln Cys Ile Asn Val Gln Tyr Asp Thr Asn Pro 
1               5                   10                  15  


<210> 45
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 45
Lys Pro Asn Val Asp Phe Trp His Gly Gly Gln Cys Asn Phe Thr 
1               5                   10                  15  


<210> 46
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 46
Phe Gln Ile Gly Gln Cys Tyr Gly Thr Gln His Trp Ala Ser Phe 
1               5                   10                  15  


<210> 47
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 47
Trp Gln Tyr Glu Asn Trp Ala Ile Thr Lys Trp Gln Cys Val Thr 
1               5                   10                  15  


<210> 48
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 48
Ser Asp Gly Trp Ala Lys Gln Cys Trp Ala Pro Thr His Asn Thr 
1               5                   10                  15  


<210> 49
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 49
Gly Gln Asp Trp His Gly Gln Gln Cys Cys Ala Glu Tyr Ala Glu 
1               5                   10                  15  


<210> 50
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 50
Ile Pro His Gln Tyr Ala Pro Gly Gln Cys Cys Lys Ile Ala Tyr 
1               5                   10                  15  


<210> 51
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 51
Thr Leu Gly Gln Cys Cys Ala Phe Pro Val Leu Asp Trp Lys Asn 
1               5                   10                  15  


<210> 52
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 52
Ala Leu Gly Glu Cys Val Lys Pro Leu Ala Phe Glu Lys Gln Ser 
1               5                   10                  15  


<210> 53
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 53
Val Ile Gly Phe Cys Ala Lys Pro Gln Asp Asn Ser Ser Ala Pro 
1               5                   10                  15  


<210> 54
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 54
Pro Ser Pro Trp Ala Thr Cys Asp Phe 
1               5                   


<210> 55
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 55
Pro Ser Pro Phe Ala Thr Cys Asp Phe 
1               5                   


<210> 56
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 56
Asp Phe Trp His Gly Glu Thr Cys Lys Val Thr Gln Phe Asp Gln 
1               5                   10                  15  


<210> 57
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 57
Asp Phe Trp His Gly Glu Gln Cys Lys Val Thr Gln Phe Asp Gln 
1               5                   10                  15  


<210> 58
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 58
Asn Asp Tyr Gln Tyr Lys Gly Gln Gln Cys Leu Asp Pro Lys Tyr 
1               5                   10                  15  


<210> 59
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 59
His Asn Trp Ala Ala Gln Cys Leu Asn Pro Asp Gln Ala Lys Leu 
1               5                   10                  15  


<210> 60
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 60
His Asn Trp Ala Ala Gln Cys Leu Lys Asp Gln Ala Lys Leu 
1               5                   10                  


<210> 61
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 61
Ser Ile Ile Gly Gln Cys Tyr Lys Lys Asp Pro Ser Gln Ala Thr 
1               5                   10                  15  


<210> 62
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 62
Ser Ile Ile Gly Met Cys Tyr Lys Lys Asp Pro Ser 
1               5                   10          


<210> 63
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 63
Val Ile Gly Gln Cys Leu Lys Asn 
1               5               


<210> 64
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 64
Trp Gln Tyr Glu Asn Trp Ala Ile Thr Lys Trp Gln Cys Val Lys 
1               5                   10                  15  


<210> 65
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 65
Val Ile Val Gly Gln Cys Leu Lys Lys 
1               5                   


<210> 66
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 66
Val Ile Leu Gly Gln Cys Leu Lys Gln 
1               5                   


<210> 67
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 67
Tyr Val Tyr Gly Met Cys Trp Lys Asp Tyr Thr Asp Thr Asp Lys 
1               5                   10                  15  


<210> 68
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 68
Tyr Val Tyr Gly Met Cys Trp Lys Asp Tyr Thr Thr Asp Lys 
1               5                   10                  


<210> 69
<211> 25
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 69
Glu Gly Val Lys Leu Thr Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser 
1               5                   10                  15      


Met Asp Ser Asp Asn Ser Met Ser Val 
            20                  25  


<210> 70
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 70
Asp Phe Trp His Gly Asp Thr Cys Lys Val Thr Gln Phe Asp Gln 
1               5                   10                  15  


<210> 71
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 71
Glu Gly Val Lys Leu Thr Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser 
1               5                   10                  15      


<210> 72
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 72
Gly Val Lys Leu Thr Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser Met 
1               5                   10                  15      


<210> 73
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 73
Val Lys Leu Thr Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser Met Asp 
1               5                   10                  15      


<210> 74
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 74
Lys Leu Thr Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser Met Asp Ser 
1               5                   10                  15      


<210> 75
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 75
Leu Thr Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser Met Asp Ser Asp 
1               5                   10                  15      


<210> 76
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 76
Thr Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser Met Asp Ser Asp Asn 
1               5                   10                  15      


<210> 77
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 77
Ala Leu Asn Asp Ser Ser Leu Asp Leu Ser Met Asp Ser Asp Asn Ser 
1               5                   10                  15      


<210> 78
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 78
Leu Asn Asp Ser Ser Leu Asp Leu Ser Met Asp Ser Asp Asn Ser Met 
1               5                   10                  15      


<210> 79
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 79
Asn Asp Ser Ser Leu Asp Leu Ser Met Asp Ser Asp Asn Ser Met Ser 
1               5                   10                  15      


<210> 80
<211> 16
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 80
Asp Ser Ser Leu Asp Leu Ser Met Asp Ser Asp Asn Ser Met Ser Val 
1               5                   10                  15      



