                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
       Wisconsin Alumni Research Foundation
       Ronald Raines
       Ian Windsor
 
<120>  CIRCULAR STRAINED ZYMOGEN COMPOSITIONS AND METHODS OF USE

<130>  M0656.70469WO00

<140>  Not yet assigned
<141>  Concurrently herewith

<150>  US 62/868,841
<151>  2019-06-28

<160>  50    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (4)..(5)
<223>  Cleavage site

<400>  1

Ser Gly Ile Phe Leu Glu Thr Ser 
1               5               


<210>  2
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  2

Lys Glu Ser Arg Ala Lys Lys Phe Gln Arg Gln His Met Asp Ser Asp 
1               5                   10                  15      


Ser Ser Pro Ser Ser Ser Ser Thr Tyr Cys Asn Gln Met Met Arg Arg 
            20                  25                  30          


Arg Asn Met Thr Gln Gly Arg Cys Lys Pro Val Asn Thr Phe Val His 
        35                  40                  45              


Glu Pro Leu Val Asp Val Gln Met Val Cys Phe Gln Glu Lys Val Thr 
    50                  55                  60                  


Cys Lys Asn Gly Gln Gly Asn Cys Tyr Lys Ser Asn Ser Ser Met His 
65                  70                  75                  80  


Ile Thr Asp Cys Arg Leu Thr Asn Gly Ser Arg Tyr Pro Asn Cys Ala 
                85                  90                  95      


Tyr Arg Thr Ser Pro Lys Glu Arg His Ile Ile Val Ala Cys Glu Gly 
            100                 105                 110         


Ser Pro Tyr Val Pro Val His Phe Asp Ala Ser Val Glu Asp Ser Thr 
        115                 120                 125             


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  3

Lys Glu Ser Arg 
1               


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  4

Lys Glu Ser Arg Ala 
1               5   


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  5

Lys Glu Ser Arg Ala Lys 
1               5       


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  6

Glu Asp Ser Thr 
1               


<210>  7
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  7

Val Glu Asp Ser Thr 
1               5   


<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  8

Ser Val Glu Asp Ser Thr 
1               5       


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  9

Lys Pro Ile Glu Phe Leu Glu Leu Lys 
1               5                   


<210>  10
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  10

Lys Pro Ile Glu Phe 
1               5   


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  11

Leu Glu Leu Lys 
1               


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  12

Thr Ala Thr Ile Met Met Gln Arg Gly Asn 
1               5                   10  


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  13

Thr Ala Thr Ile Met 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  14

Met Gln Arg Gly Asn 
1               5   


<210>  15
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (6)..(7)
<223>  Cleavage site

<400>  15

Glu Asp Val Val Cys Cys Ser Met Ser Tyr 
1               5                   10  


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (6)..(7)
<223>  Cleavage site

<400>  16

Glu Asp Val Val Ala Cys Ser Met Ser Tyr 
1               5                   10  


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (4)..(5)
<223>  Cleavage site

<400>  17

Ala Val Leu Gln Ser Gly Phe Arg 
1               5               


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (4)..(5)
<223>  Cleavage site

<400>  18

Ala Val Leu Gln Ser Gly Phe Arg 
1               5               


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (4)..(5)
<223>  Cleavage site

<400>  19

Ala Thr Leu Gln Ser Gly Asn Ala 
1               5               


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (3)..(4)
<223>  Cleavage site

<400>  20

Pro Leu Gly Leu Ala Gly 
1               5       


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (4)..(5)
<223>  Cleavage site

<400>  21

Ile Pro Val Ser Leu Arg Ser Gly 
1               5               


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  22

Pro Pro Val Ile Leu Pro Ile Gln Ala 
1               5                   


<210>  23
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  23

Gly Gly Gly Ser Gly Ile Phe Leu Glu Thr Ser Gly Gly Gly 
1               5                   10                  


<210>  24
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  24

Gly Gly Ser Gly Ile Phe Leu Glu Thr Ser Gly Gly 
1               5                   10          


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  25

Gly Ser Gly Ile Phe Leu Glu Thr Ser Gly 
1               5                   10  


<210>  26
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  26

Gly Ser Gly Ile Phe Leu Glu Thr Ser Leu 
1               5                   10  


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  27

Gly Ser Gly Val Phe Val Glu Thr Ser Leu 
1               5                   10  


<210>  28
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (7)..(8)
<223>  Cleavage site

<400>  28

Ser Gly Gly Ser Gly Val Tyr His Val Ser Thr Leu Val Pro Glu Phe 
1               5                   10                  15      


<210>  29
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  29

Gly Ser Gly Val Phe Val Glu Met Pro Leu 
1               5                   10  


<210>  30
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (7)..(8)
<223>  Cleavage site

<400>  30

Ser Gly Gly Ser Gly Val Phe Val Val Asn Gly Leu Val Pro Glu Phe 
1               5                   10                  15      


<210>  31
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  31

Gly Ser Gly Asn Tyr Leu Val Thr Ser Leu 
1               5                   10  


<210>  32
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  32

Gly Ser Gly Val Tyr Leu Ala Thr Asp Leu 
1               5                   10  


<210>  33
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (7)..(8)
<223>  Cleavage site

<400>  33

Ser Gly Gly Ser Gly Ile Met Phe Glu Ser Asn Leu Val Pro Glu Phe 
1               5                   10                  15      


<210>  34
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  34

Gly Ser Gly Ile Met Phe Gln Ser Ala Leu 
1               5                   10  


<210>  35
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  35

Gly Ser Gly Asn Tyr Phe Val Gln Gly Leu 
1               5                   10  


<210>  36
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  36

Gly Ser Gly Asn Tyr Phe Val Glu Thr Leu 
1               5                   10  


<210>  37
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  37

Ile Arg Lys Ile Leu Phe Leu Asp Gly 
1               5                   


<210>  38
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  38

Val Ser Gln Asn Tyr Pro Ile Val Gln Asn 
1               5                   10  


<210>  39
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide


<220>
<221>  SITE
<222>  (5)..(6)
<223>  Cleavage site

<400>  39

Gly Ser Gly Leu Phe Thr Glu Tyr Gly Leu 
1               5                   10  


<210>  40
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  40

His Phe Asp Ala Ser Val Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg 
1               5                   10                  15      


Gln His 
        


<210>  41
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  41

His Phe Asp Ala Ser Val Gly Ser Thr Ala Thr Ile Met Met Gln Arg 
1               5                   10                  15      


Gly Asn Ala Gly Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His 
            20                  25                  30          


<210>  42
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  42

Gly His Asp Ala Ser Val Glu Asp Ser Thr Lys Glu Ser Arg Ala Lys 
1               5                   10                  15      


Lys Phe Gln Arg Gln His 
            20          


<210>  43
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  43

His Gly Asp Ala Ser Val Gly Gly Gly Ser Gly Ile Phe Leu Glu Thr 
1               5                   10                  15      


Ser Gly Gly Gly Lys Glu Ser Arg Ala Lys Lys Phe Gln Arg Gln His 
            20                  25                  30          


<210>  44
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  44

His Phe Asp Ala Ser Val Gly Gly Ser Gly Ile Phe Leu Glu Thr Ser 
1               5                   10                  15      


Gly Gly Lys Glu Ser Arg Ala Lys Lys Phe Gln Arg Gln His 
            20                  25                  30  


<210>  45
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  45

His Gly Asp Ala Ser Val Gly Ser Gly Ile Phe Leu Glu Thr Ser Gly 
1               5                   10                  15      


Lys Glu Ser Arg Ala Lys Lys Phe Gln Arg Gln His 
            20                  25              


<210>  46
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  46

His Phe Asp Ala Ser Val Ser Gly Ile Phe Leu Glu Thr Ser Lys Glu 
1               5                   10                  15      


Ser Arg Ala Lys Lys Phe Gln Arg Gln His 
            20                  25      


<210>  47
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  47

His Gly Asp Ala Ser Gly Ile Phe Leu Glu Thr Ser Arg Ala Lys Lys 
1               5                   10                  15      


Phe Gln Arg Gln His 
            20      


<210>  48
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  48

His Gly Asp Ala Ser Gly Ile Phe Leu Glu Thr Ser Ala Lys Lys Phe 
1               5                   10                  15      


Gln Arg Gln His 
            20  


<210>  49
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  49

His Phe Asp Ala Ser Gly Ile Phe Leu Glu Thr Ser Lys Lys Phe Gln 
1               5                   10                  15      


Arg Gln His 
            


<210>  50
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  50

His Gly Asp Ala Ser Gly Ile Phe Leu Glu Thr Ser Lys Phe Gln Arg 
1               5                   10                  15      


Gln His 
        


