                         SEQUENCE LISTING

<110>  Eutilex Co., Ltd.
 
<120>  CHIMERIC ANTIGEN RECEPTOR WITH 4-1BB COSTIMULATORY DOMAIN

<130>  47683-0044WO1

<150>  62/867,503
<151>  2019-06-27

<150>  PCT/KR2019/010244
<151>  2019-08-12

<150>  16/715,462
<151>  2019-12-16

<150>  62/991,493
<151>  2020-03-18

<150>  63/004,827
<151>  2020-04-03

<150>  63/043,237
<151>  2020-06-24

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  15
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  1
cgtttctctg ttgtt                                                        15


<210>  2
<211>  141
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  2
cgtttctctg ttgttaaacg gggcagaaag aaactcctgt atatattcaa acaaccattt       60

atgagaccag tacaaactac tcaagaggaa gatggctgta gctgccgatt tccagaagaa      120

gaagaaggag gatgtgaact g                                                141


<210>  3
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide

<400>  3

Arg Phe Ser Val Val 
1               5   


<210>  4
<211>  47
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polyptpdie

<400>  4

Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
1               5                   10                  15      


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
            20                  25                  30          


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40                  45          


<210>  5
<211>  207
<212>  DNA
<213>  Artificial

<220>
<223>  CD8/Hinge/Transmembrane

<400>  5
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgctag ccagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc      180

ctgtcactgg ttatcaccct ttactgc                                          207


<210>  6
<211>  339
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  6
agagtgaagt tcagcaggag cgcagacgcc cccgcgtaca agcagggcca gaaccagctc       60

tataacgagc tcaatctagg acgaagagag gagtacgatg ttttggacaa gagacgtggc      120

cgggaccctg agatgggggg aaagccgaga aggaagaacc ctcaggaagg cctgtacaat      180

gaactgcaga aagataagat ggcggaggcc tacagtgaga ttgggatgaa aggcgagcgc      240

cggaggggca aggggcacga tggcctttac cagggtctca gtacagccac caaggacacc      300

tacgacgccc ttcacatgca ggccctgccc cctcgctaa                             339


<210>  7
<211>  69
<212>  DNA
<213>  Artificial

<220>
<223>  CD8 alpha  leader sequence

<400>  7
ggatccatgg ccttaccagt gaccgccttg ctcctgccgc tggccttgct gctccacgcc       60

gccaggccg                                                               69


<210>  8
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  Flag-tag sequence

<400>  8
gactacaagg acgacgatga caag                                              24


<210>  9
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain CDR1

<400>  9

Arg Ser Ser Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu His 
1               5                   10                  15      


<210>  10
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain CDR2

<400>  10

Lys Val Ser Asn Arg Phe Ser 
1               5           


<210>  11
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  Light chain CDR3

<400>  11

Ser Gln Asn Thr His Val Pro Pro Thr 
1               5                   


<210>  12
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain CDR1

<400>  12

Asp Tyr Glu Met His 
1               5   


<210>  13
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain CDR2

<400>  13

Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  14
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Heavy chain CDR3

<400>  14

Phe Tyr Ser Tyr Thr Tyr 
1               5       


<210>  15
<211>  112
<212>  PRT
<213>  Artificial

<220>
<223>  human GC33 light chain variable region

<400>  15

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Gly Asn Thr Tyr Leu His Trp Tyr Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  16
<211>  115
<212>  PRT
<213>  Artificial

<220>
<223>  human GC33 heavy chain variable region

<400>  16

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
            20                  25                  30          


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
    50                  55                  60                  


Lys Gly Arg Ala Thr Leu Thr Ala Asp Lys Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  17
<211>  336
<212>  DNA
<213>  Artificial

<220>
<223>  human GC33 light chain variable region

<400>  17
gacgtcgtta tgacacagag tcccctctcc ttgccggtga ccctgggtca gcctgcgtcc       60

atctcttgca gatcctccca gtctctggta cactccaacg gcaacacata cttgcactgg      120

taccaacaaa gacctggtca gtcaccgcga cttctcatat ataaagtttc caataggttc      180

agtggagtgc cagacaggtt cagtggttca ggatcaggca ctgatttcac gcttaaaatc      240

agtcgggttg aggcggagga cgtaggagtt tactattgca gccagaatac gcacgtgccg      300

cctacttttg gctctggaac caagttggaa ataaag                                336


<210>  18
<211>  345
<212>  DNA
<213>  Artificial

<220>
<223>  human GC33 heavy chain variable region

<400>  18
caagtgcaac tcgtacaatc aggtgctgaa gtcaaaaagc cgggagcctc tgttaaagtg       60

tcctgtaaag ccagcggcta cacctttacc gattatgaga tgcactgggt tcggcaggct      120

ccgggccaag gtctggagtg gatcggggct cttgacccaa agacgggcga cacggcttat      180

tcacaaaaat tcaaaggtag ggctactctg actgccgata agtccaccag caccgcgtat      240

atggagctct ctagcttgcg aagcgaggac acggcggtgt actattgcac acgcttctat      300

agttacacat attggggtca aggcacgctt gtgaccgtgt ctagc                      345


<210>  19
<211>  243
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide

<400>  19

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Asp His Ile Asn Asn Trp 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Ser Gly Ala Thr Ser Leu Glu Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Lys Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Trp Ser Thr Pro Phe 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Phe Ser Leu Ser Arg Tyr Ser Val His Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Met Ile Trp Gly Gly 
                165                 170                 175     


Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ser 
            180                 185                 190         


Lys Asp Asn Ser Lys Asn Gln Val Ser Leu Lys Leu Ser Ser Val Thr 
        195                 200                 205             


Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Asn Glu Gly Asp Thr 
    210                 215                 220                 


Thr Ala Gly Thr Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser 
            


<210>  20
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  20
gatattcaga tgacccagtc cccgagctcc ctgtccgcct ctgtgggcga tagggtcacc       60

atcacctgca aggccagtga ccacatcaac aactggctgg cctggtatca acagaaacca      120

ggaaaagctc cgaaactact gatcagcggc gccacctctc tggaaaccgg agtcccttct      180

cgcttctctg gttccggatc tgggaaggat tacactctga ccatcagcag tctgcagccg      240

gaagacttcg caacttatta ctgtcagcag tactggtcca cccccttcac cttcggacag      300

ggtaccaagg tggagatcaa aggcggaggc ggatctggcg gcggaggaag tggcggaggg      360

ggatctcagg tgcagctgca ggagtcgggc ccaggactgg tgaagccttc ggagaccctg      420

tccctcacct gcactgtctc tggtttctcc ctgagtcggt actctgtgca ttggatccgg      480

cagcccccag ggaagggact ggagtggctg gggatgatct ggggaggcgg cagcaccgac      540

tacaacagcg ccctgaagtc ccgactgacc atatcaaagg acaactccaa gaaccaggtg      600

tccttgaagc tgagctctgt gaccgctgcg gacacggccg tgtattactg tgcgagaaat      660

gagggcgata ccaccgccgg cacttggttt gcctattggg gccagggaac cctggtcacc      720

gtctcctca                                                              729


<210>  21
<211>  242
<212>  PRT
<213>  Artificial

<220>
<223>  synthetic polypeptide

<400>  21

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln Asp Ile Ser Lys Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr His Thr Ser Arg Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr Gly Gly Gly Gly Ser 
            100                 105                 110         


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Lys Leu Gln Glu 
        115                 120                 125             


Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser Leu Ser Val Thr Cys 
    130                 135                 140                 


Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly Val Ser Trp Ile Arg 
145                 150                 155                 160 


Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Gly Ser 
                165                 170                 175     


Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser Arg Leu Thr Ile Ile 
            180                 185                 190         


Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys Met Asn Ser Leu Gln 
        195                 200                 205             


Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys His Tyr Tyr Tyr Gly 
    210                 215                 220                 


Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 
225                 230                 235                 240 


Ser Ser 
        


<210>  22
<211>  726
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  22
gacatccaga tgacacagac tacatcctcc ctgtctgcct ctctgggaga cagagtcacc       60

atcagttgca gggcaagtca ggacattagt aaatatttaa attggtatca gcagaaacca      120

gatggaactg ttaaactcct gatctaccat acatcaagat tacactcagg agtcccatca      180

aggttcagtg gcagtgggtc tggaacagat tattctctca ccattagcaa cctggagcaa      240

gaagatattg ccacttactt ttgccaacag ggtaatacgc ttccgtacac gttcggaggg      300

gggaccaagc tggagatcac aggtggcggt ggctcgggcg gtggtgggtc gggtggcggc      360

ggatctgagg tgaaactgca ggagtcagga cctggcctgg tggcgccctc acagagcctg      420

tccgtcacat gcactgtctc aggggtctca ttacccgact atggtgtaag ctggattcgc      480

cagcctccac gaaagggtct ggagtggctg ggagtaatat ggggtagtga aaccacatac      540

tataattcag ctctcaaatc cagactgacc atcatcaagg acaactccaa gagccaagtt      600

ttcttaaaaa tgaacagtct gcaaactgat gacacagcca tttactactg tgccaaacat      660

tattactacg gtggtagcta tgctatggac tactggggcc aaggaacctc agtcaccgtc      720

tcctca                                                                 726


<210>  23
<211>  1184
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  23
cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt       60

tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg      120

aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa      180

gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa      240

gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt      300

gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg      360

ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg      420

cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg      480

ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt      540

tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg      600

gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc      660

tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg      720

tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg      780

caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat      840

ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct      900

ttccgtcctc agccgtcgct tcatgtgact ccactgagta ccgggcgccg tccaggcacc      960

tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg     1020

cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga     1080

tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc     1140

agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtga                      1184


<210>  24
<211>  84
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  24
agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta       60

gtcagtgtgg aaaatctcta gcag                                              84


<210>  25
<211>  1377
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  25
cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc       60

ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt      120

tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga      180

gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat      240

taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt      300

tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag      360

gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa      420

ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa      480

gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg      540

gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta      600

gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga      660

gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg      720

ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg      780

agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc      840

caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg      900

ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat      960

aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac     1020

aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat     1080

gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca     1140

aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga     1200

atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg     1260

tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa     1320

ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtat        1377


<210>  26
<211>  547
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  26
tagactgtag cccaggaata tggcagctag attgtacaca tttagaagga aaagttatct       60

tggtagcagt tcatgtagcc agtggatata tagaagcaga agtaattcca gcagagacag      120

ggcaagaaac agcatacttc ctcttaaaat tagcaggaag atggccagta aaaacagtac      180

atacagacaa tggcagcaat ttcaccagta ctacagttaa ggccgcctgt tggtgggcgg      240

ggatcaagca ggaatttggc attccctaca atccccaaag tcaaggagta atagaatcta      300

tgaataaaga attaaagaaa attataggac aggtaagaga tcaggctgaa catcttaaga      360

cagcagtaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt      420

acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac      480

aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag      540

tttggct                                                                547


<210>  27
<211>  591
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  27
atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc       60

cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta      120

tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt      180

ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg      240

gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta      300

ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt      360

tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg      420

cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca      480

atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc      540

gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct g               591


<210>  28
<211>  98
<212>  DNA
<213>  Artificial

<220>
<223>  synthetic oligonucleotide

<400>  28
gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca       60

ctgcttaagc ctcaataaag cttgccttga gtgcttca                               98


