                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  TNF muteins and uses thereof

<130>  20.11.142841/02

<150>  GB1909390.5
<151>  2019-06-28

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  157
<212>  PRT
<213>  Homo sapiens

<400>  1

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  2
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNF Mutein G4

<400>  2

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ser Ser Thr Tyr Asn Pro Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  3
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF Mutein B5

<400>  3

Val His Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Thr Gly Thr Tyr Val Asp Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  4
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF muteins B6

<400>  4

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Thr Gly Thr Tyr Ser Tyr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  5
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein F3

<400>  5

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Thr Thr Thr Tyr Arg Leu Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Val Ala Leu 
145                 150                 155         


<210>  6
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein F7

<400>  6

Val His Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ser Glu Thr Val Val Gly Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Val Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  7
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein B2

<400>  7

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Thr Ala Ser Tyr Ser Gly Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  8
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein B4

<400>  8

Val Arg Ser Ser Ser His Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ser Glu Ser His Asp Gly Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  9
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein C4

<400>  9

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ser His Ser Val Glu Gly Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  10
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein C8

<400>  10

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ser Gln Thr His Asp Gly Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  11
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein C9

<400>  11

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Ile Asn Gly Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Thr Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Asp 
    130                 135                 140                 


Ala Asp Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  12
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  G4 domain

<400>  12

Ser Ser Thr Tyr Asn Pro 
1               5       


<210>  13
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B5 domain

<400>  13

Thr Gly Thr Tyr Val Asp 
1               5       


<210>  14
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B6 domain

<400>  14

Thr Gly Thr Tyr Ser Tyr 
1               5       


<210>  15
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  F3 domain

<400>  15

Thr Thr Thr Tyr Arg Leu 
1               5       


<210>  16
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  F7 domain

<400>  16

Ser Glu Thr Val Val Gly 
1               5       


<210>  17
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B2 domain

<400>  17

Thr Ala Ser Tyr Ser Gly 
1               5       


<210>  18
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  B4 domain

<400>  18

Ser Glu Ser His Asp Gly 
1               5       


<210>  19
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C4 domain

<400>  19

Ser His Ser Val Glu Gly 
1               5       


<210>  20
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C8 domain

<400>  20

Ser Gln Thr His Asp Gly 
1               5       


<210>  21
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C9 domain

<400>  21

Ala Val Thr Tyr Gln Thr 
1               5       


<210>  22
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is A, G, S, T, E, H, Q, D, I, L or M

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is Y, V or H

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is S, V, R, N, D, E, I, L, M or T

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is G, D, Y, L, P, E, A, V, I, M, F or W

<400>  22

Xaa Xaa Xaa Xaa Xaa Xaa 
1               5       


<210>  23
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is A, G, S, T, E, H or Q

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is Y, V or H

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is S, V, R, N, D or E

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is G, D, Y, L or P

<400>  23

Xaa Xaa Xaa Xaa Xaa Xaa 
1               5       


<210>  24
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is A, G, S, T or E

<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is Y or V

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is S, V, R or N

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is G, D, Y, L or P

<400>  24

Xaa Xaa Xaa Xaa Xaa Xaa 
1               5       


<210>  25
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is G, S, T or E

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  X is Y or V

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is S, V, R or N

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is G, D, Y, L or P

<400>  25

Xaa Xaa Thr Xaa Xaa Xaa 
1               5       


<210>  26
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is G, S or T

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is S, V, R or N

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is D, Y, L or P

<400>  26

Xaa Xaa Thr Tyr Xaa Xaa 
1               5       


<210>  27
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF motif


<220>
<221>  VARIANT
<222>  (1)..(1)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (2)..(2)
<223>  X is G or S

<220>
<221>  VARIANT
<222>  (5)..(5)
<223>  X is S, V or N

<220>
<221>  VARIANT
<222>  (6)..(6)
<223>  X is D, Y or P

<400>  27

Xaa Xaa Thr Tyr Xaa Xaa 
1               5       


<210>  28
<211>  157
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNF mutein consensus


<220>
<221>  VARIANT
<222>  (84)..(84)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (85)..(85)
<223>  X is A, G, S, T, E, H, Q, D, I, L or M

<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  X is S or T

<220>
<221>  VARIANT
<222>  (87)..(87)
<223>  X is Y, V or H

<220>
<221>  VARIANT
<222>  (88)..(88)
<223>  X is S, V, R, N, D, E, I, L, M or T

<220>
<221>  VARIANT
<222>  (89)..(89)
<223>  X is G, D, Y, L, P, E, A, V, I, M, F or W

<400>  28

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Xaa Xaa Xaa Xaa Xaa Xaa Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  29
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  29

Leu Asn Arg Arg 
1               


<210>  30
<211>  4
<212>  PRT
<213>  Homo sapiens

<400>  30

Phe Ala Glu Ser 
1               


<210>  31
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TNFR1 antagonist motif

<400>  31

Ser Thr Thr His Asn Gln 
1               5       


<210>  32
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein G4

<400>  32
gttcgttctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatct ctagtaccta taatcccaaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


<210>  33
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein B5

<400>  33
gttcattctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatca ctgggaccta tgttgataaa gttaatctgc tgagcgctat taagagcccg      300

aggttgcccg agcacgcatg tgctgctgac ccacaccatt tcacgcatca ctgggaccta      360

tgttgataaa gttaatctgc tgagcgctat taagagcccg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


<210>  34
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein B6

<400>  34
gttcgttctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatca ctgggaccta ttcgtataaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


<210>  35
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein F3

<400>  35
gttcgttctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatca ctacgaccta taggcttaaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcgttgcgtt g               471


<210>  36
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein F7

<400>  36
gttcattctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatca gtgagaccgt ggttggtaaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg tagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


<210>  37
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein B2

<400>  37
gttcgttctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatca ctgcgtccta tagtggtaaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


<210>  38
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein B4

<400>  38
gttcgttctt cctctcatac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatca gtgagtccca tgatggtaaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


<210>  39
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein C4

<400>  39
gttcgttctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatca gtcattccgt ggagggtaaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


<210>  40
<211>  471
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TNF mutein C8

<400>  40
gttcgttctt cctctcgtac cccatctgat aaacctgttg cccatgtcgt tgccaacccg       60

caggcagaag gccaactgca atggctgaat cgccgtgcaa atgcgctgct ggctaatggt      120

gttgagctgc gtgacaacca actggttgtg ccaagcgaag gcctgtatct gatctatagc      180

caggtgctgt ttaaaggtca aggttgcccg agcacgcatg tgctgctgac ccacaccatt      240

tcacgcatct cgcagaccca tgatgggaaa gttaatctgc tgagcgctat taagagcccg      300

tgtcagcgtg aaacgccgga gggtgcagaa gcgaagccgt ggtacgagcc gatctatctg      360

ggtggcgtct ttcaactgga gaaaggtgat cgtctgagcg cagaaatcaa ccgtccggac      420

tacttggact ttgcggagtc cggtcaagtt tacttcggca tcattgcgtt g               471


