                               SEQUENCE LISTING

<110> THERMO FISHER SCIENTIFIC BALTICS UAB
      LIFE TECHNOLOGIES CORPORATION
 
<120> OLIGONUCLEOTIDE-TETHERED NUCLEOTIDES

<130> LT01475PCT

<140>
<141>

<150> 63/032,297
<151> 2020-05-29

<150> 62/864,589
<151> 2019-06-21

<160> 46    

<170> PatentIn version 3.5

<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU 

<400> 1
uagatcggaa gagcacacgt ctg                                               23


<210> 2
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU 

<400> 2
uagatcggaa gagcacacgt ctg                                               23


<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU 

<400> 3
uagatcggaa gagcacacgt ctg                                               23


<210> 4
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 4
agatcggaag agcacacgtc tg                                                22


<210> 5
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 5
taatacgact cactatag                                                     18


<210> 6
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU 

<220>
<221> modified_base
<222> (2)..(8)
<223> a, c, t, g, unknown or other

<400> 6
unnnnnnnna gatcggaaga gcgtcgtgta                                        30


<210> 7
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU 

<400> 7
uagatcggaa gagcacacgt ctgaactcca gtcacatgcc taaatctcgt atgccgtctt       60

ctgcttg                                                                 67


<210> 8
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 8
tacacgacgc tcttccgatc taacggtacg ccagaatctt g                           41


<210> 9
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 9
tacacgacgc tcttccgatc tagagccacc accggaac                               38


<210> 10
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"


<220>
<221> modified_base
<222> (22)..(31)
<223> a, c, t, g, unknown or other

<400> 10
tacacgacgc tcttccgatc tnnnnnnnnn n                                      31


<210> 11
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 11
ctctttccct acacgacgct cttccgatct aagtcgtaac aaggtaaccg                  50


<210> 12
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 12
ctctttccct acacgacgct cttccgatct ctgagccakr atcaaactct                  50


<210> 13
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 13
ctctttccct acacgacgct cttccgatct ctgaaccaag atcaaattct                  50


<210> 14
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 14
ctctttccct acacgacgct cttccgatct ctaagccagg atcaaactct                  50


<210> 15
<211> 50
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 15
ctctttccct acacgacgct cttccgatct ctgagccaga atcgaaccct                  50


<210> 16
<211> 55
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 16
aagcagtggt atcaacgcag agtacttttt tttttttttt tttttttttt ttttt            55


<210> 17
<211> 66
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 17
aatgatacgg cgaccaccga gatctacacg cctgtccgcg gaagcagtgg tatcaacgca       60

gagtac                                                                  66


<210> 18
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 18
caagcagaag acggcatacg agatgtgact ggagttcaga cgtgtgctct tccgatct         58


<210> 19
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 19
caagcagaag acggcatacg a                                                 21


<210> 20
<211> 27
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<220>
<221> source
<223> /note="Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (17)..(24)
<223> a, c, t, g, unknown or other

<400> 20
ccaggaccag cgattcnnnn nnnnggg                                           27


<210> 21
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 21
cagtggtatc aacgcagagt acccaggacc agcgattc                               38


<210> 22
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU 

<400> 22
utttatatat ttattggaga ctgactacca gatgtaacac ctatagtgag tcgtattag        59


<210> 23
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (2)..(2)
<223> alkyne modified dU

<400> 23
tuatatattt attggagact gactaccaga tgtaaca                                37


<210> 24
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 24
tgcagacatg ggtaggcatc cttggcgta                                         29


<210> 25
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 25
gtacgccaag gatgcctacc catgtctgca                                        30


<210> 26
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 26
ctaatacgac tcactatagg tgttacatct ggtagtcagt ctccaataaa tatataaa         58


<210> 27
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 27
gtcgctcaac tcagctacag tacgccaagg atgcctaccc atgtctgca                   49


<210> 28
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 28
gtacgccaag gatgcctacc catgtctgca                                        30


<210> 29
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 29
ccggggatcc catgtg                                                       16


<210> 30
<211> 112
<212> RNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polynucleotide"

<400> 30
gggaaagcuu uuacauuuuc gcgauaccgu ccagcgacau ucuuccucgg uacauaaucu       60

ccuuuggcgu uucccgaugu ccgucacgca caugggaucc ccggguaccg ag              112


<210> 31
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 31
aaaaaaaaaa tacgccaagg atgcctaccc atgtctgca                              39


<210> 32
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 32
tgcagacatg ggtaggcatc cttggcgta                                         29


<210> 33
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU

<400> 33
uttatatatt tattggagac tgactaccag atgtaaca                               38


<210> 34
<211> 88
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 34
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatcttt       60

tttttttttt tttttttttt tttttttt                                          88


<210> 35
<211> 67
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (1)..(1)
<223> alkyne modified dU 

<400> 35
uagatcggaa gagcacacgt ctgaactcca gtcacatgcc taaatctcgt atgccgtctt       60

ctgcttg                                                                 67


<210> 36
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 36
gcggcgacca aatcgttgta aagatcggaa gagcgtcgtg ta                          42


<210> 37
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 37
cagacgtgtg ctcttccgat ct                                                22


<210> 38
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 38
cagacgtgtg ctcttccgat ct                                                22


<210> 39
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      primer"

<400> 39
aagtcgtaac aaggtaaccg                                                   20


<210> 40
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 40
tttttttttt tttttttttt tttttttttt                                        30


<210> 41
<211> 14
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 41
tttttttttt tttt                                                         14


<210> 42
<211> 10
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 42
aaaaaaaaaa                                                              10


<210> 43
<211> 269
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polynucleotide"


<220>
<221> misc_feature
<222> (20)..(269)
<223> /note="This region may encompass 80-250 nucleotides"

<400> 43
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      120

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      180

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaa                                        269


<210> 44
<211> 17
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"


<220>
<221> modified_base
<222> (17)..(17)
<223> a, c, t, g, unknown or other

<400> 44
tttttttttt tttttvn                                                      17


<210> 45
<211> 256
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polynucleotide"


<220>
<221> misc_feature
<222> (7)..(256)
<223> /note="This region may encompass 80-250 nucleotides"

<400> 45
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      120

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      180

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      240

aaaaaaaaaa aaaaaa                                                      256


<210> 46
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 46
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                                        30
