                         SEQUENCE LISTING

<110>  Yale University
       GUPTA, ANISHA
       GLAZER, PETER
       EGAN, MARIE
       SALTZMAN, W. MARK
       BARONE, CHRISTINA
 
<120>  PEPTIDE NUCLEIC ACID COMPOSITIONS WITH MODIFIED HOOGSTEEN BINDING
        SEGMENTS AND METHODS OF USE THEREOF

<130>  YU 7662 PCT

<150>  62/864,961
<151>  2019-06-21

<160>  110   

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide

<400>  1

Tyr Gly Arg Lys Lys Arg Arg Gln Arg Arg Arg 
1               5                   10      


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  2

Arg Lys Lys Arg Arg Gln Arg Arg Arg 
1               5                   


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  3

Arg Gln Ile Lys Ile Trp Phe Gln Asn Arg Arg Met Lys Trp Lys Lys 
1               5                   10                  15      


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  4

His His His His Arg Lys Lys Arg Arg Gln Arg Arg Arg Arg His His 
1               5                   10                  15      


His His His 
            


<210>  5
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  5

Met Val Lys Ser Lys Ile Gly Ser Trp Ile Leu Val Leu Phe Val Ala 
1               5                   10                  15      


Met Trp Ser Asp Val Gly Leu Cys Lys Lys Arg Pro Lys Pro 
            20                  25                  30  


<210>  6
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide

<400>  6

Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly 
1               5                   10                  15      


Ala Trp Ser Gln Pro Lys Lys Lys Arg Lys Val 
            20                  25          


<210>  7
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  7
gaaagaaaga ga                                                           12


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  8
tgccctgaaa gaaagaga                                                     18


<210>  9
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  9
agaatggtgc aaagagg                                                      17


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  10
acatgattag caaaaggg                                                     18


<210>  11
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  11
ctttctttct ct                                                           12


<210>  12
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  12
tctctttctt tc                                                           12


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  13
tctctttctt tcagggca                                                     18


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  14
cccttttgct aatcatgt                                                     18


<210>  15
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  15
cctctttgca ccattct                                                      17


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  Linked to lys-lys-lys

<400>  16
ntttntttnt nttctctttc tttcagggca                                        30


<210>  17
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  17
ttttnnnccc ttttgctaat catgt                                             25


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Linked to lys-lys-lys

<400>  18
tttntnncct ctttgcacca ttct                                              24


<210>  19
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n = Pseudoisocytosine

<400>  19
tnttttnttn                                                              10


<210>  20
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  20
cttcttttct                                                              10


<210>  21
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n = Pseudoisocytosine

<400>  21
ttnttntttn                                                              10


<210>  22
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  22
ctttcttctt                                                              10


<210>  23
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n = Pseudoisocytosine

<400>  23
nnntnnttnt                                                              10


<210>  24
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  24
tcttcctccc                                                              10


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Linked to lys-lys-lys

<400>  25
tnttttnttn cttcttttct                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Linked to lys-lys-lys

<400>  26
ttnttntttn ctttcttctt                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(3)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Linked to lys-lys-lys

<400>  27
nnntnnttnt tcttcctccc                                                   20


<210>  28
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  28
cttctccaaa ggagt                                                        15


<210>  29
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  29
cttctccaca ggagtcag                                                     18


<210>  30
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  30
cttctccaca ggagtcaggt gc                                                22


<210>  31
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  31
tctccttaaa cctgt                                                        15


<210>  32
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  32
tctccttaaa cctgtctt                                                     18


<210>  33
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  33
tcttctctgt ctccac                                                       16


<210>  34
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  34
tcttctctgt ctccacat                                                     18


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Linked to lys-lys-lys

<400>  35
nntnttnctt ctccaaagga gt                                                22


<210>  36
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Linked to lys-lys-lys

<400>  36
ttnntnttct ccttaaacct gt                                                22


<210>  37
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  37
ttnntnttct ccttaaacct gtctt                                             25


<210>  38
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  Linked to lys-lys-lys

<400>  38
tntnttnttc ttctctgtct ccac                                              24


<210>  39
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  39
nntnttnctt ctccacagga gtcag                                             25


<210>  40
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Linked to lys-lys-lys

<400>  40
nntnttnctt ctccacagga gtcaggtgc                                         29


<210>  41
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  Linked to lys-lys-lys

<400>  41
tntnttnttc ttctctgtct ccacat                                            26


<210>  42
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  42
gttcagcgtg tccggcgagg gcgaggtgag tctatgggac ccttgatgtt t                51


<210>  43
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  43
aaacatcaag ggtcccatag actcacctcg ccctcgccgg acacgctgaa c                51


<210>  44
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  44
cttgccccac agggcagtaa cggcagattt ttcttccggc gttaaatgca ccatggtgtc       60

tgtttgaggt                                                              70


<210>  45
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  45
acagacacca tggtgcacct gactcctgag gagaagtctg ccgttactgc c                51


<210>  46
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (62)..(63)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (63)..(64)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (64)..(65)
<223>  Optional phosphorothioate internucleoside linkage

<400>  46
ttgccccaca gggcagtaac ggcagacttc tcctcaggag tcaggtgcac catggtgtct       60

gtttg                                                                   65


<210>  47
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  47
tttcctctat gggtaag                                                      17


<210>  48
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  48
cttacccata gaggaaa                                                      17


<210>  49
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  49
atgccaacta gaagagg                                                      17


<210>  50
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  50
cctcttctag ttggcat                                                      17


<210>  51
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  51
ctttcccttg tatctttt                                                     18


<210>  52
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  52
aaaagataca agggaaag                                                     18


<210>  53
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  53
gaaggagaaa                                                              10


<210>  54
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (3)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Linked to lys-lys-lys

<400>  54
ttnnntttnc tttcccttgt atctttt                                           27


<210>  55
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  55
agaaaaaagg                                                              10


<210>  56
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  56
cttcctcttt                                                              10


<210>  57
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  57
tttctccttc                                                              10


<210>  58
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  58
tttctccttc agtgttca                                                     18


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  59
tccttttgct cacctgtggt                                                   20


<210>  60
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  60
tcttttttcc                                                              10


<210>  61
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  61
ccttttttct                                                              10


<210>  62
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  62
ccttttttct ggctaagt                                                     18


<210>  63
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<400>  63
nttnntnttt                                                              10


<210>  64
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n = Pseudoisocytosine

<400>  64
tnttttttnn                                                              10


<210>  65
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  Linked to lys-lys-lys

<400>  65
nttnntnttt tttctccttc agtgttca                                          28


<210>  66
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Linked to lys-lys-lys

<400>  66
ttttnnttcc ttttgctcac ctgtggt                                           27


<210>  67
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  Linked to lys-lys-lys

<400>  67
tnttttttnn ccttttttct ggctaagt                                          28


<210>  68
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (58)..(59)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (59)..(60)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  Optional phosphorothioate internucleoside linkage

<400>  68
tcttgggatt caataacctt gcagacagtg gaggaaggcc tttggcgtga taccacaggt       60

g                                                                       61


<210>  69
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  69
tttttctgta atttttaa                                                     18


<210>  70
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  70
tctttctctg caaactt                                                      17


<210>  71
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  71
tttctttaag aacgagca                                                     18


<210>  72
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  72
tntttttttt ttctgtaatt tttaa                                             25


<210>  73
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  Linked to lys-lys-lys

<400>  73
tntntttntt ctttctctgc aaactt                                            26


<210>  74
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  74
tttntttttt ctttaagaac gagca                                             25


<210>  75
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (2)..(3)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (61)..(62)
<223>  Optional phosphorothioate internucleoside linkage

<220>
<221>  misc_feature
<222>  (62)..(63)
<223>  Optional phosphorothioate internucleoside linkage

<400>  75
tccaagtttg cagagaaaga taatatagtc cttggagaag gaggaatcac cctgagtgga       60

ggt                                                                     63


<210>  76
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  76
ctcttcttct                                                              10


<210>  77
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  77
tcttcttctc                                                              10


<210>  78
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  78
tcttcttctc atttc                                                        15


<210>  79
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (10)..(11)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  79
ntnttnttnt tcttcttctc atttc                                             25


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  Linked to lys-lys-lys

<400>  80
nttnttcttc ttctcatttc                                                   20


<210>  81
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  81
attcccgagt agcagatgac catgacagct tagggcagga ccagccccaa gatgactatc       60


<210>  82
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  82
tttaggattc ccgagtagca gatgacccct cagagcagcg gcaggaccag ccccaagatg       60


<210>  83
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  83
gggacggcgc ccacataggc caaattcaat tgctgatccc agcttaagac gtactggtca       60

gcctggc                                                                 67


<210>  84
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  Linked to lys-lys-lys

<400>  84
nttttnnncc cttttcaagg tgagtag                                           27


<210>  85
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid

<400>  85
tcttatatct gtactcatca taggaaacac caaagataat gttctccttg atagtacccg       60

g                                                                       61


<210>  86
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  86
ccctctaatt ctctgctggc agatc                                             25


<210>  87
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  87
aaagaataac agtgataatt tctgggttaa ggcaatagca atatctctgc atataaatat       60


<210>  88
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  88
cctggcacca ttaaggagaa cattatctt                                         29


<210>  89
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (4)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  89
tntnnttttt tcctctatgg gtaag                                             25


<210>  90
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (8)..(9)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  Linked to lys-lys-lys

<400>  90
tnttntnncc tcttctagtt ggcat                                             25


<210>  91
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  91
ttctgtatct atattcatca taggaaacac caaagataat gttctcctta atggtgccag       60

g                                                                       61


<210>  92
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  92
gatgactatc tttaatgtct ggaaattctt ccagaattaa ttaagactgt atggaaaatg       60

agagc                                                                   65


<210>  93
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  93
ccccaagatg actatcttta atgtctggaa cgatcatcag aattgatact gactgtatgg       60

aaaatg                                                                  66


<210>  94
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  94
gatgactatc tttaatgtct ggaaattcta ctagaattga tactgactgt atggaaaatg       60

agagc                                                                   65


<210>  95
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  95
tccccttggt gaagg                                                        15


<210>  96
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Peptide Nucleic Acid


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (3)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (7)..(8)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (22)..(22)
<223>  Linked to lys-lys-lys

<400>  96
ttnnnnttcc ccttggtgaa gg                                                22


<210>  97
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic PRimer

<400>  97
aggacggtcc cggcctgcga cacttccgcc cataattgtt cttcatctgc ggggcggggg       60

ggg                                                                     63


<210>  98
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  98
tcttccgagc ag                                                           12


<210>  99
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Linked to lys-lys-lys

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  Linked by three 8-amino-2, 6, 10-trioxaoctanoic acid, three 
       8-amino-3,6-dioxaoctanoic acid, three 6-aminohexanoic acid , or 
       three 11-amino-3,6,9-trioxaundecanoic acid molecules

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  Linked to lys-lys-lys

<400>  99
nnttnttctt ccgagcag                                                     18


<210>  100
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  100
ctgctcggaa ga                                                           12


<210>  101
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer

<400>  101
ccttcaccaa gggga                                                        15


<210>  102
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  102
gcctggcacc attaaagaaa atatcattgg                                        30


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  103
gttctcagtt ttcctggatt                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  104
tgatgacgct tctgtatcta                                                   20


<210>  105
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  105
agaacattat ctttggtgtt tcc                                               23


<210>  106
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  106
aaatatcatt ggtgtttcct atga                                              24


<210>  107
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  107
acaccgacca ttaaagaaaa tatcatg                                           27


<210>  108
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide

<400>  108
aaaacatgat attttcttta atggtcg                                           27


<210>  109
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n = Pseudoisocytosine

<400>  109
ntnttnttnt                                                              10


<210>  110
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  n = Pseudoisocytosine

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  n = Pseudoisocytosine

<400>  110
ntttntttnt nt                                                           12


