                               SEQUENCE LISTING

<110> TRUSTEES OF BOSTON COLLEGE
 
<120> ENHANCED PLATFORMS FOR UNNATURAL AMINO ACID INCORPORATION IN 
      MAMMALIAN CELLS

<130> 0342.0008WO1

<140>
<141>

<150> 62/864,570
<151> 2019-06-21

<160> 49    

<170> PatentIn version 3.5

<210> 1
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 1
ggaaaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguuuccgc ca                                                           72


<210> 2
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 2
gggcggcuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggcugcccgc ca                                                           72


<210> 3
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 3
gggugacuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gguugcccgc ca                                                           72


<210> 4
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 4
ggggggcuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggcuccccgc ca                                                           72


<210> 5
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 5
gggcggcuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gguugcccgc ca                                                           72


<210> 6
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 6
gggcgccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gggcgcccgc ca                                                           72


<210> 7
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 7
ggggagguga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gccuccccgc ca                                                           72


<210> 8
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 8
ggggaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguccccgc ca                                                           72


<210> 9
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 9
ggccggguga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gccuggccgc ca                                                           72


<210> 10
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 10
ggggaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguccucgc ca                                                           72


<210> 11
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 11
ggggcccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggggucccgc ca                                                           72


<210> 12
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 12
gggggccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gggcucccgc ca                                                           72


<210> 13
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 13
gggguccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gggaucccgc ca                                                           72


<210> 14
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 14
ggggaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguucccgc ca                                                           72


<210> 15
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 15
ggggagguga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gucuucccgc ca                                                           72


<210> 16
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 16
ggggggguga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gcccccucgc ca                                                           72


<210> 17
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 17
ggugggguga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gcccuaccgc ca                                                           72


<210> 18
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 18
gggggucuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggacucccgc ca                                                           72


<210> 19
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 19
gggucccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggggguccgc ca                                                           72


<210> 20
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 20
ggggaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguucucgc ca                                                           72


<210> 21
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 21
gggcggcuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggccgcccgc ca                                                           72


<210> 22
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 22
gagcaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gggugcucgc ca                                                           72


<210> 23
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 23
ggggggguga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gcccccccgc ca                                                           72


<210> 24
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 24
gaggggguga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

gcccccucgc ca                                                           72


<210> 25
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 25
gggagccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguucccgc ca                                                           72


<210> 26
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 26
gggagccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguucccgc ca                                                           72


<210> 27
<211> 72
<212> RNA
<213> Methanosarcina barkeri

<400> 27
gaggaccuga ucauguagau cgaacggacu cuaaauccgu ucagccgggu uagauucccg       60

ggguucucgc ca                                                           72


<210> 28
<211> 87
<212> RNA
<213> Escherichia coli

<400> 28
gcccggaugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgcuccg gguacca                                           87


<210> 29
<211> 87
<212> RNA
<213> Escherichia coli

<400> 29
gcccggaugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgcuccg ggcacca                                           87


<210> 30
<211> 87
<212> RNA
<213> Escherichia coli

<400> 30
gcccggaugg uggaaucggu agacacaagg gacucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgcuccg ggcacca                                           87


<210> 31
<211> 87
<212> RNA
<213> Escherichia coli

<400> 31
gggcgugugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccgcg cccacca                                           87


<210> 32
<211> 87
<212> RNA
<213> Escherichia coli

<400> 32
gggcgcgugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccgcg cccacca                                           87


<210> 33
<211> 87
<212> RNA
<213> Escherichia coli

<400> 33
gggcaugugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccaug cccacca                                           87


<210> 34
<211> 87
<212> RNA
<213> Escherichia coli

<400> 34
gggcacgugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccgug cccacca                                           87


<210> 35
<211> 87
<212> RNA
<213> Escherichia coli

<400> 35
gggggugugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccgcc cccacca                                           87


<210> 36
<211> 87
<212> RNA
<213> Escherichia coli

<400> 36
gggggcgugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccguc cccacca                                           87


<210> 37
<211> 87
<212> RNA
<213> Escherichia coli

<400> 37
ggggaugugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccguc cccacca                                           87


<210> 38
<211> 87
<212> RNA
<213> Escherichia coli

<400> 38
ggggacgugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccguc cccacca                                           87


<210> 39
<211> 87
<212> RNA
<213> Escherichia coli

<400> 39
gcccguaugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgcugcg ggcacca                                           87


<210> 40
<211> 87
<212> RNA
<213> Escherichia coli

<400> 40
gggauagugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccuau cccacca                                           87


<210> 41
<211> 87
<212> RNA
<213> Escherichia coli

<400> 41
gggcaugugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccgug cccacca                                           87


<210> 42
<211> 87
<212> RNA
<213> Escherichia coli

<400> 42
gggcagaugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgcucug cccacca                                           87


<210> 43
<211> 87
<212> RNA
<213> Escherichia coli

<400> 43
gggcguaugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgcugcg cccacca                                           87


<210> 44
<211> 87
<212> RNA
<213> Escherichia coli

<400> 44
gggcaagugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgccuug cccacca                                           87


<210> 45
<211> 87
<212> RNA
<213> Escherichia coli

<400> 45
gcacacaugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgcugug ugcacca                                           87


<210> 46
<211> 735
<212> PRT
<213> Adeno-associated virus - 2

<400> 46
Met Ala Ala Asp Gly Tyr Leu Pro Asp Trp Leu Glu Asp Thr Leu Ser 
1               5                   10                  15      


Glu Gly Ile Arg Gln Trp Trp Lys Leu Lys Pro Gly Pro Pro Pro Pro 
            20                  25                  30          


Lys Pro Ala Glu Arg His Lys Asp Asp Ser Arg Gly Leu Val Leu Pro 
        35                  40                  45              


Gly Tyr Lys Tyr Leu Gly Pro Phe Asn Gly Leu Asp Lys Gly Glu Pro 
    50                  55                  60                  


Val Asn Glu Ala Asp Ala Ala Ala Leu Glu His Asp Lys Ala Tyr Asp 
65                  70                  75                  80  


Arg Gln Leu Asp Ser Gly Asp Asn Pro Tyr Leu Lys Tyr Asn His Ala 
                85                  90                  95      


Asp Ala Glu Phe Gln Glu Arg Leu Lys Glu Asp Thr Ser Phe Gly Gly 
            100                 105                 110         


Asn Leu Gly Arg Ala Val Phe Gln Ala Lys Lys Arg Val Leu Glu Pro 
        115                 120                 125             


Leu Gly Leu Val Glu Glu Pro Val Lys Thr Ala Pro Gly Lys Lys Arg 
    130                 135                 140                 


Pro Val Glu His Ser Pro Val Glu Pro Asp Ser Ser Ser Gly Thr Gly 
145                 150                 155                 160 


Lys Ala Gly Gln Gln Pro Ala Arg Lys Arg Leu Asn Phe Gly Gln Thr 
                165                 170                 175     


Gly Asp Ala Asp Ser Val Pro Asp Pro Gln Pro Leu Gly Gln Pro Pro 
            180                 185                 190         


Ala Ala Pro Ser Gly Leu Gly Thr Asn Thr Met Ala Thr Gly Ser Gly 
        195                 200                 205             


Ala Pro Met Ala Asp Asn Asn Glu Gly Ala Asp Gly Val Gly Asn Ser 
    210                 215                 220                 


Ser Gly Asn Trp His Cys Asp Ser Thr Trp Met Gly Asp Arg Val Ile 
225                 230                 235                 240 


Thr Thr Ser Thr Arg Thr Trp Ala Leu Pro Thr Tyr Asn Asn His Leu 
                245                 250                 255     


Tyr Lys Gln Ile Ser Ser Gln Ser Gly Ala Ser Asn Asp Asn His Tyr 
            260                 265                 270         


Phe Gly Tyr Ser Thr Pro Trp Gly Tyr Phe Asp Phe Asn Arg Phe His 
        275                 280                 285             


Cys His Phe Ser Pro Arg Asp Trp Gln Arg Leu Ile Asn Asn Asn Trp 
    290                 295                 300                 


Gly Phe Arg Pro Lys Arg Leu Asn Phe Lys Leu Phe Asn Ile Gln Val 
305                 310                 315                 320 


Lys Glu Val Thr Gln Asn Asp Gly Thr Thr Thr Ile Ala Asn Asn Leu 
                325                 330                 335     


Thr Ser Thr Val Gln Val Phe Thr Asp Ser Glu Tyr Gln Leu Pro Tyr 
            340                 345                 350         


Val Leu Gly Ser Ala His Gln Gly Cys Leu Pro Pro Phe Pro Ala Asp 
        355                 360                 365             


Val Phe Met Val Pro Gln Tyr Gly Tyr Leu Thr Leu Asn Asn Gly Ser 
    370                 375                 380                 


Gln Ala Val Gly Arg Ser Ser Phe Tyr Cys Leu Glu Tyr Phe Pro Ser 
385                 390                 395                 400 


Gln Met Leu Arg Thr Gly Asn Asn Phe Thr Phe Ser Tyr Thr Phe Glu 
                405                 410                 415     


Asp Val Pro Phe His Ser Ser Tyr Ala His Ser Gln Ser Leu Asp Arg 
            420                 425                 430         


Leu Met Asn Pro Leu Ile Asp Gln Tyr Leu Tyr Tyr Leu Ser Arg Thr 
        435                 440                 445             


Asn Thr Pro Ser Gly Thr Thr Thr Gln Ser Arg Leu Gln Phe Ser Gln 
    450                 455                 460                 


Ala Gly Ala Ser Asp Ile Arg Asp Gln Ser Arg Asn Trp Leu Pro Gly 
465                 470                 475                 480 


Pro Cys Tyr Arg Gln Gln Arg Val Ser Lys Thr Ser Ala Asp Asn Asn 
                485                 490                 495     


Asn Ser Glu Tyr Ser Trp Thr Gly Ala Thr Lys Tyr His Leu Asn Gly 
            500                 505                 510         


Arg Asp Ser Leu Val Asn Pro Gly Pro Ala Met Ala Ser His Lys Asp 
        515                 520                 525             


Asp Glu Glu Lys Phe Phe Pro Gln Ser Gly Val Leu Ile Phe Gly Lys 
    530                 535                 540                 


Gln Gly Ser Glu Lys Thr Asn Val Asp Ile Glu Lys Val Met Ile Thr 
545                 550                 555                 560 


Asp Glu Glu Glu Ile Arg Thr Thr Asn Pro Val Ala Thr Glu Gln Tyr 
                565                 570                 575     


Gly Ser Val Ser Thr Asn Leu Gln Arg Gly Asn Arg Gln Ala Ala Thr 
            580                 585                 590         


Ala Asp Val Asn Thr Gln Gly Val Leu Pro Gly Met Val Trp Gln Asp 
        595                 600                 605             


Arg Asp Val Tyr Leu Gln Gly Pro Ile Trp Ala Lys Ile Pro His Thr 
    610                 615                 620                 


Asp Gly His Phe His Pro Ser Pro Leu Met Gly Gly Phe Gly Leu Lys 
625                 630                 635                 640 


His Pro Pro Pro Gln Ile Leu Ile Lys Asn Thr Pro Val Pro Ala Asn 
                645                 650                 655     


Pro Ser Thr Thr Phe Ser Ala Ala Lys Phe Ala Ser Phe Ile Thr Gln 
            660                 665                 670         


Tyr Ser Thr Gly Gln Val Ser Val Glu Ile Glu Trp Glu Leu Gln Lys 
        675                 680                 685             


Glu Asn Ser Lys Arg Trp Asn Pro Glu Ile Gln Tyr Thr Ser Asn Tyr 
    690                 695                 700                 


Asn Lys Ser Val Asn Val Asp Phe Thr Val Asp Thr Asn Gly Val Tyr 
705                 710                 715                 720 


Ser Glu Pro Arg Pro Ile Gly Thr Arg Tyr Leu Thr Arg Asn Leu 
                725                 730                 735 


<210> 47
<211> 87
<212> DNA
<213> Escherichia coli

<400> 47
gcccggatgg tggaatcggt agacacaagg gattctaaat ccctcggcgt tcgcgctgtg       60

cgggttcaag tcccgctccg ggtacca                                           87


<210> 48
<211> 87
<212> DNA
<213> Escherichia coli

<400> 48
gcccggatgg tggaatcggt agacacaagg gactctaaat ccctcggcgt tcgcgctgtg       60

cgggttcaag tcccgctccg ggcacca                                           87


<210> 49
<211> 87
<212> RNA
<213> Escherichia coli

<400> 49
gcccggaugg uggaaucggu agacacaagg gauucuaaau cccucggcgu ucgcgcugug       60

cggguucaag ucccgguccg gguacca                                           87


