                         SEQUENCE LISTING

<110>  CITRANVI BIOSCIENCES, LLC
 
<120>  RATIONALLY ENGINEERED CARRIER PROTEINS FOR VACCINES

<130>  12063-002WO1

<150>  US 62/862,916
<151>  2019-06-18

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  375
<212>  DNA
<213>  Vibrio cholerae

<400>  1
atgattaaat taaaatttgg tgtttttttt acagttttac tatcttcagc atatgcaaat       60

ggaacacctc aaaatattac tgatttgtgt gcagaatacc acaacacaca aatacatacg      120

ctaaatgata agatattttc gtatacagaa tctctagctg gaaaaagaga gatggctatc      180

attactttta agaatggtgc aacttttcaa gtagaagtac caggtagtca acatatagat      240

tcacaaaaaa aagcgattga aaggatgaag gataccctga ggattgcata tcttactgaa      300

gctaaagtcg aaaagttatg tgtatggaat aataaaacgc ctcatgcgat tgccgcaatt      360

agtatggcaa attaa                                                       375


<210>  2
<211>  124
<212>  PRT
<213>  Vibrio cholerae

<400>  2

Met Ile Lys Leu Lys Phe Gly Val Phe Phe Thr Val Leu Leu Ser Ser 
1               5                   10                  15      


Ala Tyr Ala Asn Gly Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu 
            20                  25                  30          


Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp Lys Ile Phe Ser Tyr 
        35                  40                  45              


Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys 
    50                  55                  60                  


Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala 
                85                  90                  95      


Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala Asn 
        115                 120                 


<210>  3
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide used in gene assembly cloning

<400>  3
gtttaacttt aagaaggaga tatacc                                            26


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide used in gene assembly cloning

<400>  4
cgaattcgag ctccgtcgac aagct                                             25


<210>  5
<211>  411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing His tag, linker, antigen, protease 
       recognition, cholera toxin subunit

<400>  5
atgcaccacc atcatcacca tggtggtggc tcatggtcac acccacagtt tgagaaaggt       60

ggcggatcgg aaaacctgta tttccaaggt gggggatcga cccctcagaa cattaccgat      120

ctttgcgcgg agtatcataa tacccaaatt cacacactta atgacaagat tttttcgtac      180

actgagtcac ttgcgggaaa gcgcgaaatg gcgatcatca cctttaagaa cggagcgacc      240

ttccaggtag aagttcccgg gagtcagcac atcgacagcc agaagaaagc tattgaacgc      300

atgaaagaca ctttacgcat tgcatattta accgaggcca aagtggaaaa gctgtgcgtg      360

tggaacaaca aaacgcctca cgcaattgcg gcaattagca tggctaatta a               411


<210>  6
<211>  136
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing His tag, linker, antigen, protease 
       recognition, cholera toxin subunit

<400>  6

Met His His His His His His Gly Gly Gly Ser Trp Ser His Pro Gln 
1               5                   10                  15      


Phe Glu Lys Gly Gly Gly Ser Glu Asn Leu Tyr Phe Gln Gly Gly Gly 
            20                  25                  30          


Ser Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His Asn Thr 
        35                  40                  45              


Gln Ile His Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu Ser Leu 
    50                  55                  60                  


Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly Ala Thr 
65                  70                  75                  80  


Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys 
                85                  90                  95      


Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu Thr Glu 
            100                 105                 110         


Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro His Ala 
        115                 120                 125             


Ile Ala Ala Ile Ser Met Ala Asn 
    130                 135     


<210>  7
<211>  345
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing His tag, linker, antigen, cholera 
       toxin subunit

<400>  7
atgcatcacc atcaccatca cggcggcgga tccacacctc aaaatattac tgatttgtgt       60

gcagaatacc acaacacaca aatacatacg ctaaatgata agatattttc gtatacagaa      120

tctctagctg gaaaaagaga gatggctatc attactttta agaatggtgc aacttttcaa      180

gtagaagtac caggtagtca acatatagat tcacaaaaaa aagcgattga aaggatgaag      240

gataccctga ggattgcata tcttactgaa gctaaagtcg aaaagttatg tgtatggaat      300

aataaaacgc ctcatgcgat tgccgcaatt agtatggcaa attaa                      345


<210>  8
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing His tag, linker, antigen, cholera 
       toxin subunit

<400>  8

Met His His His His His His Gly Gly Gly Ser Thr Pro Gln Asn Ile 
1               5                   10                  15      


Thr Asp Leu Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn 
            20                  25                  30          


Asp Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met 
        35                  40                  45              


Ala Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro 
    50                  55                  60                  


Gly Ser Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys 
65                  70                  75                  80  


Asp Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu 
                85                  90                  95      


Cys Val Trp Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met 
            100                 105                 110         


Ala Asn 
        


<210>  9
<211>  309
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized cholera toxin subunit

<400>  9
actccccaaa atatcacaga tttatgcgca gagtaccata atacccaaat ccacacgttg       60

aatgacaaga ttttcagcta cacggaatcc ttggctggta agcgtgagat ggcgattatt      120

acgttcaaaa acggcgctac ttttcaggtt gaagttccgg gcagtcaaca tatcgactca      180

cagaaaaaag ccatcgagcg catgaaggat actttgcgta tcgcgtattt gactgaagct      240

aaggtggaaa agttgtgcgt ctggaacaac aagactccac acgcgattgc cgctatttcg      300

atggctaat                                                              309


<210>  10
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon optimized fusion containing linker, His tag

<400>  10
gggggaggtt cagggggcgg atcgggcggt ggctcacacc atcaccacca ccat             54


<210>  11
<211>  363
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon optimized fusion containing cholera toxin 
       subunit, linker, His tag

<400>  11
actccccaaa atatcacaga tttatgcgca gagtaccata atacccaaat ccacacgttg       60

aatgacaaga ttttcagcta cacggaatcc ttggctggta agcgtgagat ggcgattatt      120

acgttcaaaa acggcgctac ttttcaggtt gaagttccgg gcagtcaaca tatcgactca      180

cagaaaaaag ccatcgagcg catgaaggat actttgcgta tcgcgtattt gactgaagct      240

aaggtggaaa agttgtgcgt ctggaacaac aagactccac acgcgattgc cgctatttcg      300

atggctaatg ggggaggttc agggggcgga tcgggcggtg gctcacacca tcaccaccac      360

cat                                                                    363


<210>  12
<211>  63
<212>  DNA
<213>  Escherichia coli

<400>  12
atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag       60

gcc                                                                     63


<210>  13
<211>  21
<212>  PRT
<213>  Escherichia coli

<400>  13

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  14
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated E. coli sequence

<400>  14
atggttaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag       60

gcc                                                                     63


<210>  15
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutated E. coli sequence

<400>  15

Met Val Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  16
<211>  66
<212>  DNA
<213>  Erwinia carotovora

<400>  16
atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggcc                                                                  66


<210>  17
<211>  22
<212>  PRT
<213>  Erwinia carotovora

<400>  17

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  18
<211>  78
<212>  DNA
<213>  Escherichia coli

<400>  18
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgcc                                                     78


<210>  19
<211>  26
<212>  PRT
<213>  Escherichia coli

<400>  19

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  20
<211>  429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing mutated E. coli sequence, cholera 
       toxin subunit, linker, His tag

<400>  20
atggttaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac cgtagcgcag       60

gccactcccc aaaatatcac agatttatgc gcagagtacc ataataccca aatccacacg      120

ttgaatgaca agattttcag ctacacggaa tccttggctg gtaagcgtga gatggcgatt      180

attacgttca aaaacggcgc tacttttcag gttgaagttc cgggcagtca acatatcgac      240

tcacagaaaa aagccatcga gcgcatgaag gatactttgc gtatcgcgta tttgactgaa      300

gctaaggtgg aaaagttgtg cgtctggaac aacaagactc cacacgcgat tgccgctatt      360

tcgatggcta atgggggagg ttcagggggc ggatcgggcg gtggctcaca ccatcaccac      420

caccattaa                                                              429


<210>  21
<211>  142
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing mutated E. coli sequence, cholera 
       toxin subunit, linker, His tag

<400>  21

Met Val Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala Glu 
            20                  25                  30          


Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp Lys Ile Phe Ser Tyr 
        35                  40                  45              


Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe Lys 
    50                  55                  60                  


Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile Asp 
65                  70                  75                  80  


Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile Ala 
                85                  90                  95      


Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn Lys 
            100                 105                 110         


Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala Asn Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Ser His His His His His His 
    130                 135                 140         


<210>  22
<211>  432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing Erwinia sequence, cholera toxin 
       subunit, linker, His tag

<400>  22
atgaaatacc tgctgccgac cgctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggccactc cccaaaatat cacagattta tgcgcagagt accataatac ccaaatccac      120

acgttgaatg acaagatttt cagctacacg gaatccttgg ctggtaagcg tgagatggcg      180

attattacgt tcaaaaacgg cgctactttt caggttgaag ttccgggcag tcaacatatc      240

gactcacaga aaaaagccat cgagcgcatg aaggatactt tgcgtatcgc gtatttgact      300

gaagctaagg tggaaaagtt gtgcgtctgg aacaacaaga ctccacacgc gattgccgct      360

atttcgatgg ctaatggggg aggttcaggg ggcggatcgg gcggtggctc acaccatcac      420

caccaccatt aa                                                          432


<210>  23
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing Erwinia sequence, cholera toxin 
       subunit, linker, His tag

<400>  23

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Thr Pro Gln Asn Ile Thr Asp Leu Cys Ala 
            20                  25                  30          


Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp Lys Ile Phe Ser 
        35                  40                  45              


Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile Thr Phe 
    50                  55                  60                  


Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln His Ile 
65                  70                  75                  80  


Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu Arg Ile 
                85                  90                  95      


Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp Asn Asn 
            100                 105                 110         


Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala Asn Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Ser Gly Gly Gly Ser His His His His His His 
    130                 135                 140             


<210>  24
<211>  444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, His tag

<400>  24
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgccac tccccaaaat atcacagatt tatgcgcaga gtaccataat      120

acccaaatcc acacgttgaa tgacaagatt ttcagctaca cggaatcctt ggctggtaag      180

cgtgagatgg cgattattac gttcaaaaac ggcgctactt ttcaggttga agttccgggc      240

agtcaacata tcgactcaca gaaaaaagcc atcgagcgca tgaaggatac tttgcgtatc      300

gcgtatttga ctgaagctaa ggtggaaaag ttgtgcgtct ggaacaacaa gactccacac      360

gcgattgccg ctatttcgat ggctaatggg ggaggttcag ggggcggatc gggcggtggc      420

tcacaccatc accaccacca ttaa                                             444


<210>  25
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, His tag

<400>  25

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Thr Pro Gln Asn Ile Thr 
            20                  25                  30          


Asp Leu Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp 
        35                  40                  45              


Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala 
    50                  55                  60                  


Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly 
65                  70                  75                  80  


Ser Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp 
                85                  90                  95      


Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys 
            100                 105                 110         


Val Trp Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala 
        115                 120                 125             


Asn Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser His His His 
    130                 135                 140                 


His His His 
145         


<210>  26
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Genetically modified cross-reacting material (CRM197) of 
       diphtheria toxin

<400>  26

Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 
1               5                   10                  15      


Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala 
            20                  25                  30  


<210>  27
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized cross-reacting material (CRM197) of diphtheria 
       toxin

<400>  27
tcaatcttgc cgggcattgg cagcgtgatg ggaatcgctg acggggcggt acaccacaac       60

actgaggaga tcgttgccca gtctattgct                                        90


<210>  28
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Genetically modified cross-reacting material (CRM197) of 
       diphtheria toxin

<400>  28

Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe Gln Val Val His 
1               5                   10                  15      


Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly 
            20                  25          


<210>  29
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized cross-reacting material (CRM197) of diphtheria 
       toxin

<400>  29
gcatataatt ttgtggagtc aattatcaat ctttttcagg tggtacacaa ctcttacaac       60

cgtccagcat acagccccgg t                                                 81


<210>  30
<211>  615
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, CRM sequence, 
       cholera toxin subunit, CRM sequence, linker, His tag

<400>  30
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgcctc aatcttgccg ggcattggca gcgtgatggg aatcgctgac      120

ggggcggtac accacaacac tgaggagatc gttgcccagt ctattgctac tccccaaaat      180

atcacagatt tatgcgcaga gtaccataat acccaaatcc acacgttgaa tgacaagatt      240

ttcagctaca cggaatcctt ggctggtaag cgtgagatgg cgattattac gttcaaaaac      300

ggcgctactt ttcaggttga agttccgggc agtcaacata tcgactcaca gaaaaaagcc      360

atcgagcgca tgaaggatac tttgcgtatc gcgtatttga ctgaagctaa ggtggaaaag      420

ttgtgcgtct ggaacaacaa gactccacac gcgattgccg ctatttcgat ggctaatgca      480

tataattttg tggagtcaat tatcaatctt tttcaggtgg tacacaactc ttacaaccgt      540

ccagcataca gccccggtgg gggaggttca gggggcggat cgggcggtgg ctcacaccat      600

caccaccacc attaa                                                       615


<210>  31
<211>  204
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion E. coli sequence, CRM sequence, cholera toxin 
       subunit, CRM sequence, linker, His tag

<400>  31

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Ser Ile Leu Pro Gly Ile 
            20                  25                  30          


Gly Ser Val Met Gly Ile Ala Asp Gly Ala Val His His Asn Thr Glu 
        35                  40                  45              


Glu Ile Val Ala Gln Ser Ile Ala Thr Pro Gln Asn Ile Thr Asp Leu 
    50                  55                  60                  


Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp Lys Ile 
65                  70                  75                  80  


Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile 
                85                  90                  95      


Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln 
            100                 105                 110         


His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu 
        115                 120                 125             


Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp 
    130                 135                 140                 


Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala Asn Ala 
145                 150                 155                 160 


Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe Gln Val Val His Asn 
                165                 170                 175     


Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly Gly Gly Gly Ser Gly Gly 
            180                 185                 190         


Gly Ser Gly Gly Gly Ser His His His His His His 
        195                 200                 


<210>  32
<211>  14
<212>  PRT
<213>  Influenza B virus

<400>  32

Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Gly Gly 
1               5                   10                  


<210>  33
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized Influenza B virus sequence

<400>  33
cgcggtcttt ttggggcaat cgcaggattc atcgaaggag ga                          42


<210>  34
<211>  486
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, Influenza B virus
       sequence, cholera toxin subunit, linker, His tag

<400>  34
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgcccg cggtcttttt ggggcaatcg caggattcat cgaaggagga      120

actccccaaa atatcacaga tttatgcgca gagtaccata atacccaaat ccacacgttg      180

aatgacaaga ttttcagcta cacggaatcc ttggctggta agcgtgagat ggcgattatt      240

acgttcaaaa acggcgctac ttttcaggtt gaagttccgg gcagtcaaca tatcgactca      300

cagaaaaaag ccatcgagcg catgaaggat actttgcgta tcgcgtattt gactgaagct      360

aaggtggaaa agttgtgcgt ctggaacaac aagactccac acgcgattgc cgctatttcg      420

atggctaatg ggggaggttc agggggcgga tcgggcggtg gctcacacca tcaccaccac      480

cattaa                                                                 486


<210>  35
<211>  161
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, Influenza B virus
       sequence, cholera toxin subunit, linker, His tag

<400>  35

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Arg Gly Leu Phe Gly Ala 
            20                  25                  30          


Ile Ala Gly Phe Ile Glu Gly Gly Thr Pro Gln Asn Ile Thr Asp Leu 
        35                  40                  45              


Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp Lys Ile 
    50                  55                  60                  


Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala Ile Ile 
65                  70                  75                  80  


Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly Ser Gln 
                85                  90                  95      


His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp Thr Leu 
            100                 105                 110         


Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys Val Trp 
        115                 120                 125             


Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala Asn Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser His His His His His 
145                 150                 155                 160 


His 
    


<210>  36
<211>  32
<212>  PRT
<213>  SARS-CoV-2 virus

<400>  36

Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
1               5                   10                  15      


Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
            20                  25                  30          


<210>  37
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon optimized fusion containing SARS-CoV-2 virus 
       sequence, linker

<400>  37
ggcgggaact ataactacct gtaccgtctg ttccgtaagt caaacctgaa accatttgaa       60

cgcgatatct caacagagat ctaccaagcc ggatccggtg ggggctca                   108


<210>  38
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon-optimized fusion containing linker, His tag

<400>  38
gggggaggtt cagggggcgg atcgggcggt ggctcacacc atcaccacca ccatcatcat       60

caccac                                                                  66


<210>  39
<211>  564
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, SARS-CoV-2 virus 
       sequence, linker, cholera toxin subunit, His tag

<400>  39
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgccgg cgggaactat aactacctgt accgtctgtt ccgtaagtca      120

aacctgaaac catttgaacg cgatatctca acagagatct accaagccgg atccggtggg      180

ggctcaactc cccaaaatat cacagattta tgcgcagagt accataatac ccaaatccac      240

acgttgaatg acaagatttt cagctacacg gaatccttgg ctggtaagcg tgagatggcg      300

attattacgt tcaaaaacgg cgctactttt caggttgaag ttccgggcag tcaacatatc      360

gactcacaga aaaaagccat cgagcgcatg aaggatactt tgcgtatcgc gtatttgact      420

gaagctaagg tggaaaagtt gtgcgtctgg aacaacaaga ctccacacgc gattgccgct      480

atttcgatgg ctaatggggg aggttcaggg ggcggatcgg gcggtggctc acaccatcac      540

caccaccatc atcatcacca ctaa                                             564


<210>  40
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, SARS-CoV-2 virus 
       sequence, linker, cholera toxin subunit, His tag

<400>  40

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Gly Gly Asn Tyr Asn Tyr 
            20                  25                  30          


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
        35                  40                  45              


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Gly Gly Gly Ser Thr Pro 
    50                  55                  60                  


Gln Asn Ile Thr Asp Leu Cys Ala Glu Tyr His Asn Thr Gln Ile His 
65                  70                  75                  80  


Thr Leu Asn Asp Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys 
                85                  90                  95      


Arg Glu Met Ala Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe Gln Val 
            100                 105                 110         


Glu Val Pro Gly Ser Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu 
        115                 120                 125             


Arg Met Lys Asp Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val 
    130                 135                 140                 


Glu Lys Leu Cys Val Trp Asn Asn Lys Thr Pro His Ala Ile Ala Ala 
145                 150                 155                 160 


Ile Ser Met Ala Asn Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser His His His His His His His His His His 
            180                 185         


<210>  41
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon optimized fusion containing linker, SARS-CoV-2 
       virus sequence

<400>  41
ggtgggggct caggcgggaa ctataactac ctgtaccgtc tgttccgtaa gtcaaacctg       60

aaaccatttg aacgcgatat ctcaacagag atctaccaag ccggatcc                   108


<210>  42
<211>  564
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  42
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgccac tccccaaaat atcacagatt tatgcgcaga gtaccataat      120

acccaaatcc acacgttgaa tgacaagatt ttcagctaca cggaatcctt ggctggtaag      180

cgtgagatgg cgattattac gttcaaaaac ggcgctactt ttcaggttga agttccgggc      240

agtcaacata tcgactcaca gaaaaaagcc atcgagcgca tgaaggatac tttgcgtatc      300

gcgtatttga ctgaagctaa ggtggaaaag ttgtgcgtct ggaacaacaa gactccacac      360

gcgattgccg ctatttcgat ggctaatggt gggggctcag gcgggaacta taactacctg      420

taccgtctgt tccgtaagtc aaacctgaaa ccatttgaac gcgatatctc aacagagatc      480

taccaagccg gatccggggg aggttcaggg ggcggatcgg gcggtggctc acaccatcac      540

caccaccatc atcatcacca ctaa                                             564


<210>  43
<211>  187
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  43

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Thr Pro Gln Asn Ile Thr 
            20                  25                  30          


Asp Leu Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp 
        35                  40                  45              


Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala 
    50                  55                  60                  


Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly 
65                  70                  75                  80  


Ser Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp 
                85                  90                  95      


Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys 
            100                 105                 110         


Val Trp Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala 
        115                 120                 125             


Asn Gly Gly Gly Ser Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
    130                 135                 140                 


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
145                 150                 155                 160 


Tyr Gln Ala Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser His His His His His His His His His His 
            180                 185         


<210>  44
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon optimized fusion containing linker, SARS-CoV-2 
       virus sequence

<400>  44
ggtgggggct caggaggagg ctcaggcggc ggatcgggcg ggaactataa ctacctgtac       60

cgtctgttcc gtaagtcaaa cctgaaacca tttgaacgcg atatctcaac agagatctac      120

caagccggat cc                                                          132


<210>  45
<211>  576
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  45
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgccac tccccaaaat atcacagatt tatgcgcaga gtaccataat      120

acccaaatcc acacgttgaa tgacaagatt ttcagctaca cggaatcctt ggctggtaag      180

cgtgagatgg cgattattac gttcaaaaac ggcgctactt ttcaggttga agttccgggc      240

agtcaacata tcgactcaca gaaaaaagcc atcgagcgca tgaaggatac tttgcgtatc      300

gcgtatttga ctgaagctaa ggtggaaaag ttgtgcgtct ggaacaacaa gactccacac      360

gcgattgccg ctatttcgat ggctaatggt gggggctcag gaggaggctc aggcggcgga      420

tcgggcggga actataacta cctgtaccgt ctgttccgta agtcaaacct gaaaccattt      480

gaacgcgata tctcaacaga gatctaccaa gccggatccg ggggaggttc agggggcgga      540

tcgggcggtg gctcacacca tcaccaccac cattaa                                576


<210>  46
<211>  191
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  46

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Thr Pro Gln Asn Ile Thr 
            20                  25                  30          


Asp Leu Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp 
        35                  40                  45              


Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala 
    50                  55                  60                  


Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly 
65                  70                  75                  80  


Ser Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp 
                85                  90                  95      


Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys 
            100                 105                 110         


Val Trp Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala 
        115                 120                 125             


Asn Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser Gly Gly Gly Ser Gly Gly Gly Ser His His His His His His 
            180                 185                 190     


<210>  47
<211>  24
<212>  PRT
<213>  SARS-CoV-2 virus

<400>  47

Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
1               5                   10                  15      


Lys Pro Phe Glu Arg Asp Ile Ser 
            20                  


<210>  48
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon optimized fusion containing linker, SARS-CoV-2 
       virus sequence

<400>  48
ggtgggggct caggaggagg ctcaggcggc ggatcgggcg ggaactataa ctacctgtac       60

cgtctgttcc gtaagtcaaa cctgaaacca tttgaacgcg atatctca                   108


<210>  49
<211>  552
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  49
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgccac tccccaaaat atcacagatt tatgcgcaga gtaccataat      120

acccaaatcc acacgttgaa tgacaagatt ttcagctaca cggaatcctt ggctggtaag      180

cgtgagatgg cgattattac gttcaaaaac ggcgctactt ttcaggttga agttccgggc      240

agtcaacata tcgactcaca gaaaaaagcc atcgagcgca tgaaggatac tttgcgtatc      300

gcgtatttga ctgaagctaa ggtggaaaag ttgtgcgtct ggaacaacaa gactccacac      360

gcgattgccg ctatttcgat ggctaatggt gggggctcag gaggaggctc aggcggcgga      420

tcgggcggga actataacta cctgtaccgt ctgttccgta agtcaaacct gaaaccattt      480

gaacgcgata tctcaggggg aggttcaggg ggcggatcgg gcggtggctc acaccatcac      540

caccaccatt aa                                                          552


<210>  50
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  50

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Thr Pro Gln Asn Ile Thr 
            20                  25                  30          


Asp Leu Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp 
        35                  40                  45              


Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala 
    50                  55                  60                  


Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly 
65                  70                  75                  80  


Ser Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp 
                85                  90                  95      


Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys 
            100                 105                 110         


Val Trp Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala 
        115                 120                 125             


Asn Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
                165                 170                 175     


Ser His His His His His His 
            180             


<210>  51
<211>  14
<212>  PRT
<213>  SARS-CoV-2 virus

<400>  51

Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
1               5                   10                  


<210>  52
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant codon-optimized fusion containing linker, SARS-CoV-2 
       virus sequence

<400>  52
ggtgggggct caggaggagg ctcaggcggc ggatcgggcg ggaactataa ctacctgtac       60

cgtctgttcc gtaagtca                                                     78


<210>  53
<211>  522
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  53
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt       60

tccgcctcgg ctctcgccac tccccaaaat atcacagatt tatgcgcaga gtaccataat      120

acccaaatcc acacgttgaa tgacaagatt ttcagctaca cggaatcctt ggctggtaag      180

cgtgagatgg cgattattac gttcaaaaac ggcgctactt ttcaggttga agttccgggc      240

agtcaacata tcgactcaca gaaaaaagcc atcgagcgca tgaaggatac tttgcgtatc      300

gcgtatttga ctgaagctaa ggtggaaaag ttgtgcgtct ggaacaacaa gactccacac      360

gcgattgccg ctatttcgat ggctaatggt gggggctcag gaggaggctc aggcggcgga      420

tcgggcggga actataacta cctgtaccgt ctgttccgta agtcaggggg aggttcaggg      480

ggcggatcgg gcggtggctc acaccatcac caccaccatt aa                         522


<210>  54
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion containing E. coli sequence, cholera toxin 
       subunit, linker, SARS-CoV-2 virus sequence, His tag

<400>  54

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Thr Pro Gln Asn Ile Thr 
            20                  25                  30          


Asp Leu Cys Ala Glu Tyr His Asn Thr Gln Ile His Thr Leu Asn Asp 
        35                  40                  45              


Lys Ile Phe Ser Tyr Thr Glu Ser Leu Ala Gly Lys Arg Glu Met Ala 
    50                  55                  60                  


Ile Ile Thr Phe Lys Asn Gly Ala Thr Phe Gln Val Glu Val Pro Gly 
65                  70                  75                  80  


Ser Gln His Ile Asp Ser Gln Lys Lys Ala Ile Glu Arg Met Lys Asp 
                85                  90                  95      


Thr Leu Arg Ile Ala Tyr Leu Thr Glu Ala Lys Val Glu Lys Leu Cys 
            100                 105                 110         


Val Trp Asn Asn Lys Thr Pro His Ala Ile Ala Ala Ile Ser Met Ala 
        115                 120                 125             


Asn Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Gly Gly Gly Ser Gly 
145                 150                 155                 160 


Gly Gly Ser Gly Gly Gly Ser His His His His His His 
                165                 170             


