               SEQUENCE LISTING

<110> Technische Universiteit Eindhoven

<120> RATIOMETRIC DETECTION OF LUCIFERASE ASSAYS USING A CALIBRATOR LUCIFERASE

<130> 82347PC

<150> US62/893322
<151> 2019-08-29
<150> US62/864583
<151> 2019-06-21

<160> 6

<170> BiSSAP 1.3.6

<210> 1
<211> 11
<212> PRT
<213> Oplophorus gracilirostris


<220> 
<223> SBiT with K(d) of 190 µM

<400> 1
Val Thr Gly Tyr Arg Leu Phe Glu Glu Ile Leu 
1               5                   10      

<210> 2
<211> 11
<212> PRT
<213> Oplophorus gracilirostris


<220> 
<223> SBiT with K(d) of 0.28 µM

<400> 2
Val Thr Gly Tyr Arg Leu Phe Glu Lys Ile Leu 
1               5                   10      

<210> 3
<211> 507
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of pG-SB

<400> 3
atgggctgga gccatccgca gtttgaaaaa ggtggtagca tgacatttaa actgattatc      60

aacggcaaaa ctttaaaggg agagatcaca atagaagcgg tggatgctta ggaggcggag     120

aagattttta agcagtatgc aaatgattat ggaattgatg gtgaatggac ttatgacgac     180

gcaactaaaa ctttcacggt aacagaagaa tttacaggag gttcgggtgg gtcgggaggt     240

tctggcggct ctggaggaag tggtggtagc ggtgaattcg ccgaagcagc cgctaaagaa     300

gccgcagcaa aggaagccgc ggccaaggag gcagccgcaa aagaggccgc ggcgaaggaa     360

gcagcagcca aggcagaatt cgggggtagc ggcggctcgg ggggtagtgg tggaagcggg     420

ggttcaggcg gttctggggg taccgttacc ggctatcgtc tgtttgaaaa agagagcggc     480

ggttcacatc atcatcacca ccattaa                                         507


<210> 4
<211> 948
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of pG-LB

<400> 4
atgggctgga gccatccgca gtttgaaaaa ggtggtagca tgacatttaa actgattatc      60

aacggcaaaa ctttaaaggg agagatcaca atagaagcgg tggatgctta ggaggcggag     120

aagattttta agcagtatgc aaatgattat ggaattgatg gtgaatggac ttatgacgac     180

gcaactaaaa ctttcacggt aacagaagaa tttacaggag gttcgggtgg gtcgggaggt     240

tctggcggct ctggaggaag tggtggtagc ggtgaattcg ccgaagcagc cgctaaagaa     300

gccgcagcaa aggaagccgc ggccaaggag gcagccgcaa aagaggccgc ggcgaaggaa     360

gcagcagcca aggcagaatt cgggggtagc ggcggctcgg ggggtagtgg tggaagcggg     420

ggttcaggcg gttctggggg taccgtcttc acactcgaag atttcgttgg ggactgggaa     480

cagacagccg cctacaacct ggaccaagtc cttgaacagg gaggtgtgtc cagtttgctg     540

cagaatctcg ccgtgtccgt aactccgatc caaaggattg tccggagcgg tgaaaatgcc     600

ctgaagatcg acatccatgt catcatcccg tatgaaggtc tgagcgccga ccaaatggcc     660

cagatcgaag aggtgtttaa ggtggtgtac cctgtggatg atcatcactt taaggtgatc     720

ctgccctatg gcacactggt aatcgacggg gttacgccga acatgctgaa ctatttcgga     780

cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga tcactgtaac agggaccctg     840

tggaacggca acaaaattat cgacgagcgc ctgatcaccc ccgacggctc catgctgttc     900

cgagtaacca tcaacagcgg aggttcccac caccaccatc accactaa                  948


<210> 5
<211> 594
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of K9C/D157C/G191C variant

<400> 5
atgtggtctc atcctcaatt tgaatgcatg gtatttactc ttgaagattt tgtcggtgat      60

tggcgccaga ccgccggcta taacctggac caagtgcttg aacagggcgg ggttagcagc     120

ctgtttcaaa acctgggggt gagtgtcacg ccaattcagc gcatcgttct gtcgggagag     180

aatggtctga aaatcgatat ccacgtcatt atcccgtacg aaggtctttc tggtgatcag     240

atggggcaga tagaaaaaat attcaaagtg gtgtacccag tagacgatca tcacttcaag     300

gttatactgc actatggcac cctcgttatc gatggcgtta ctccgaatat gatcgattac     360

tttgggcgtc cttatgaagg tattgcggtg ttcgacggta aaaaaattac ggttaccggg     420

acgctctgga atggtaataa aatcattgat gagcgcttga taaacccatg tggcagcctt     480

ctgttcagag ttacgataaa cggggttacg ggttggcgac tgagcgaaag aatattagct     540

gcgtccgcac tcgagctgcc agaaaccggt tgtcaccacc accaccacca ctga           594


<210> 6
<211> 1149
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA sequence of TNFa-SB

<400> 6
atgtcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct      60

gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa     120

aagaccactc ctttaagaag gctgatggaa gcgttcgcta aaagacaggg taaggaaatg     180

gactccttaa gattcttgta cgacggtatt agaattcaag ctgatcagac ccctgaagat     240

ttggacatgg aggataacga tattattgag gctcacagag aacagattgg tggtgtgcgt     300

agcagcagcc gtaccccgag cgataaaccg gtggcgcatg tggtggcgaa tccgcaggcg     360

gaaggccagc tgcagtggct gaaccgtcgt gcgaatgcgc tgctggccaa cggcgtggaa     420

ctgcgtgata atcagctggt tgtgccgagc gaaggcctgt atctgattta tagccaggtg     480

ctgtttaaag gccagggctg cccgagcacc catgtgctgc tgacccatac cattagccgt     540

attgcggtga gctatcagac caaagtgaac ctgctgtctg cgattaaaag cccgtgccag     600

cgtgaaaccc cggaaggcgc ggaagcgaaa ccgtggtatg aaccgattta tctgggcggc     660

gtgtttcagc tggaaaaagg cgatcgtctg agcgcggaaa ttaaccgtcc ggattatctg     720

gattttgcgg aaagcggcca ggtgtatttt ggcattattg cgctgggatc cggggggagc     780

ggtggtagcg gtgggtccgg gggaagtggt ggcagtggtg ggtccggagg gagtggcggg     840

tcgggaggaa gcgggggctc aggatccggg ggcagtggcg gctcaggagg ttctggaggc     900

tcaggcggaa gcgggggtag cgaagcagcc gcaaaagaag cagccgccaa ggaagcagca     960

gcaaaggagg cagctgcaaa ggaggcggcg gcgaaagagg cggcagccaa aggaggatct    1020

ggtggctcag gtggtagtgg aggatcagga ggtagtggcg gatcaggtac cgttaccggc    1080

tatcgtctgt ttgaaaaaga gagcggtggt tcagggggct cgtggtcaca tccgcaattt    1140

gaaaagtaa                                                            1149


