<110>    BioApplications Inc.

<120>    Recombinant vectors for the preparation of antigens for
         prevention of African swine fever and uses thereof

<130>    MPCT20-060

<150>    KR 10-2019-0071879
<151>    2019-06-17

<150>    KR 10-2020-0072205
<151>    2020-06-15

<160>    11

<170>    KoPatentIn 3.0

<210>    1
<211>    339
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence of EP153 antigen


<400>    1
ggtgaaatta acaagccaat ctgctaccaa aacgatgata agattttcta ctgtcctaag         60

gattgggttg gatacaacaa cgtttgttac tactttggca acgaggaaaa gaactataac        120

aacgctagta actactgcaa gcagctgaac tcgacattga cgaacaacaa cactatcttg        180

gtgaacttaa ctaagaccct caacctcaca aagacctaca accatgagtc taactactgg        240

gtcaactact cactaattaa gaacgagtct gtgcttctta gggacagcgg gtactacaag        300

aagcagaagc acgtttcctt gctttacata tgctcaaag                               339


<210>    2
<211>    113
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of EP153 antigen


<400>    2
Gly Glu Ile Asn Lys Pro Ile Cys Tyr Gln Asn Asp Asp Lys Ile Phe
  1               5                  10                  15 

Tyr Cys Pro Lys Asp Trp Val Gly Tyr Asn Asn Val Cys Tyr Tyr Phe
             20                  25                  30 

Gly Asn Glu Glu Lys Asn Tyr Asn Asn Ala Ser Asn Tyr Cys Lys Gln
         35                  40                  45 

Leu Asn Ser Thr Leu Thr Asn Asn Asn Thr Ile Leu Val Asn Leu Thr
     50                  55                  60 

Lys Thr Leu Asn Leu Thr Lys Thr Tyr Asn His Glu Ser Asn Tyr Trp
 65                  70                  75                  80 

Val Asn Tyr Ser Leu Ile Lys Asn Glu Ser Val Leu Leu Arg Asp Ser
                 85                  90                  95 

Gly Tyr Tyr Lys Lys Gln Lys His Val Ser Leu Leu Tyr Ile Cys Ser
            100                 105                 110 

Lys
   


<210>    3
<211>    987
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence of EP402 antigen


<400>    3
tctaacattg ttcttagtat tgattactgg gtttctttta ataagacaat catcctcgat         60

tctaacatta ctaacgacaa taacgatatt aatggagtga gttggaactt ttttaacaac        120

agctttaata ctctagcaac atgcggtaaa gctggcaatt tttgcgagtg cagcaattac        180

tcaacaagta tatacaatat caccaataat tgctccctta caatatttcc tcataacgat        240

gttttcgata ctacatatca ggtcgtgtgg aatcagatta tcaactacac gatcaagctt        300

ttgacccctg ctactcctcc taacataacc tacaactgta ctaattttct gataacgtgt        360

aagaagaata acggtacaaa caccaacatt tatctgaata taaatgacac gttcgtgaaa        420

tacactaacg aaagtatcct tgagtataac tggaataact ccaatattaa taacttcact        480

gcaacttgta tcattaacaa tactatttca acttcgaacg aaacaacctt gataaattgt        540

acgtacttaa ctctttcttc caattacttc tataccttct ttaagggagg agggggttca        600

cgtaaaagaa aaaagcacgt tgaggaaatt gagtcaccac ctccagaatc taatgaggaa        660

gaacaatgtc agcatgacga tactacttct attcacgaac cgtctcctag ggagcccctg        720

ctccctaaac cttattcaag atatcagtac aacactccta tttactatat gagaccttcg        780

acccaaccac ttaacccttt cccccttcct aaaccttgcc ctccacctaa gccatgccct        840

cctccgaagc cctgtccgcc tcctaagccc tgtccaagtg ccgagtctta ctctcctcct        900

aagcctcttc cgagcatccc tcttcttccc aacattcctc cgctctccac acaaaatatt        960

agccttatac atgtagatag gattatc                                            987


<210>    4
<211>    329
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of EP402 antigen


<400>    4
Ser Asn Ile Val Leu Ser Ile Asp Tyr Trp Val Ser Phe Asn Lys Thr
  1               5                  10                  15 

Ile Ile Leu Asp Ser Asn Ile Thr Asn Asp Asn Asn Asp Ile Asn Gly
             20                  25                  30 

Val Ser Trp Asn Phe Phe Asn Asn Ser Phe Asn Thr Leu Ala Thr Cys
         35                  40                  45 

Gly Lys Ala Gly Asn Phe Cys Glu Cys Ser Asn Tyr Ser Thr Ser Ile
     50                  55                  60 

Tyr Asn Ile Thr Asn Asn Cys Ser Leu Thr Ile Phe Pro His Asn Asp
 65                  70                  75                  80 

Val Phe Asp Thr Thr Tyr Gln Val Val Trp Asn Gln Ile Ile Asn Tyr
                 85                  90                  95 

Thr Ile Lys Leu Leu Thr Pro Ala Thr Pro Pro Asn Ile Thr Tyr Asn
            100                 105                 110 

Cys Thr Asn Phe Leu Ile Thr Cys Lys Lys Asn Asn Gly Thr Asn Thr
        115                 120                 125 

Asn Ile Tyr Leu Asn Ile Asn Asp Thr Phe Val Lys Tyr Thr Asn Glu
    130                 135                 140 

Ser Ile Leu Glu Tyr Asn Trp Asn Asn Ser Asn Ile Asn Asn Phe Thr
145                 150                 155                 160 

Ala Thr Cys Ile Ile Asn Asn Thr Ile Ser Thr Ser Asn Glu Thr Thr
                165                 170                 175 

Leu Ile Asn Cys Thr Tyr Leu Thr Leu Ser Ser Asn Tyr Phe Tyr Thr
            180                 185                 190 

Phe Phe Lys Gly Gly Gly Gly Ser Arg Lys Arg Lys Lys His Val Glu
        195                 200                 205 

Glu Ile Glu Ser Pro Pro Pro Glu Ser Asn Glu Glu Glu Gln Cys Gln
    210                 215                 220 

His Asp Asp Thr Thr Ser Ile His Glu Pro Ser Pro Arg Glu Pro Leu
225                 230                 235                 240 

Leu Pro Lys Pro Tyr Ser Arg Tyr Gln Tyr Asn Thr Pro Ile Tyr Tyr
                245                 250                 255 

Met Arg Pro Ser Thr Gln Pro Leu Asn Pro Phe Pro Leu Pro Lys Pro
            260                 265                 270 

Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro Lys Pro Cys Pro Pro Pro
        275                 280                 285 

Lys Pro Cys Pro Ser Ala Glu Ser Tyr Ser Pro Pro Lys Pro Leu Pro
    290                 295                 300 

Ser Ile Pro Leu Leu Pro Asn Ile Pro Pro Leu Ser Thr Gln Asn Ile
305                 310                 315                 320 

Ser Leu Ile His Val Asp Arg Ile Ile
                325                


<210>    5
<211>    273
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence of chapherone binding protein(BiP)


<400>    5
atggctcgct cgtttggagc taacagtacc gttgtgttgg cgatcatctt cttcggtgag         60

tgattttccg atcttcttct ccgatttaga tctcctctac attgttgctt aatctcagaa        120

ccttttttcg ttgttcctgg atctgaatgt gtttgtttgc aatttcacga tcttaaaagg        180

ttagatctcg attggtattg acgattggaa tctttacgat ttcaggatgt ttatttgcgt        240

tgtcctctgc aatagaagag gctacgaagt taa                                     273


<210>    6
<211>    681
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence of pFc2


<400>    6
gttggaagac catgccctat atgtcctgct tgtgaaggtc caggtccctc tgcttttata         60

ttcccaccaa agccgaagga taccttgatg atttcacgta caccacaagt tacttgtgtt        120

gttgtggatg tttcacaaga aaatcctgag gtacaattca gctggtatgt tgatggggta        180

gaagtgcaca ctgcacagac tcgaccaaag gaggcccagt ttaactcgac ttatagagtt        240

gtttctgttc tcccaatcca acacgaagat tggctgaagg gcaaggaatt tgaatgcaag        300

gttaacaata aagatctacc agcaccaatt accaggatta tttctaaggc aaaaggaccc        360

tccagagagc cccaagttta cacattgtct ccttctgctg aggagcttag tagaagtaaa        420

gtgagcatta cctgcttagt gacgggattc taccctccag acatcgacgt cgaatggaaa        480

tctaatggtc aacctgagcc agaaggtaac tataggacta ctccaccaca acaggacgtc        540

gatggcacat actttcttta ttcaaaactt gctgtcgata aggcaagttg gcaaagagga        600

gatccatttc agtgtgctgt aatgcatgag gctttgcata atcattatac acagaaatca        660

gtttctaaaa cacaagggaa a                                                  681


<210>    7
<211>    227
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of pFc2


<400>    7
Val Gly Arg Pro Cys Pro Ile Cys Pro Ala Cys Glu Gly Pro Gly Pro
  1               5                  10                  15 

Ser Ala Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser
             20                  25                  30 

Arg Thr Pro Gln Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asn
         35                  40                  45 

Pro Glu Val Gln Phe Ser Trp Tyr Val Asp Gly Val Glu Val His Thr
     50                  55                  60 

Ala Gln Thr Arg Pro Lys Glu Ala Gln Phe Asn Ser Thr Tyr Arg Val
 65                  70                  75                  80 

Val Ser Val Leu Pro Ile Gln His Glu Asp Trp Leu Lys Gly Lys Glu
                 85                  90                  95 

Phe Glu Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Thr Arg
            100                 105                 110 

Ile Ile Ser Lys Ala Lys Gly Pro Ser Arg Glu Pro Gln Val Tyr Thr
        115                 120                 125 

Leu Ser Pro Ser Ala Glu Glu Leu Ser Arg Ser Lys Val Ser Ile Thr
    130                 135                 140 

Cys Leu Val Thr Gly Phe Tyr Pro Pro Asp Ile Asp Val Glu Trp Lys
145                 150                 155                 160 

Ser Asn Gly Gln Pro Glu Pro Glu Gly Asn Tyr Arg Thr Thr Pro Pro
                165                 170                 175 

Gln Gln Asp Val Asp Gly Thr Tyr Phe Leu Tyr Ser Lys Leu Ala Val
            180                 185                 190 

Asp Lys Ala Ser Trp Gln Arg Gly Asp Pro Phe Gln Cys Ala Val Met
        195                 200                 205 

His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Val Ser Lys Thr
    210                 215                 220 

Gln Gly Lys
225        


<210>    8
<211>    12
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence of HDEL


<400>    8
catgatgagc tc                                                             12


<210>    9
<211>    4
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Amino acid sequence of HDEL


<400>    9
His Asp Glu Leu
  1            


<210>    10
<211>    1358
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence of full EP153 construct


<400>    10
tctagaatta ttacatcaaa acaaaaaatg gctcgctcgt ttggagctaa cagtaccgtt         60

gtgttggcga tcatcttctt cggtgagtga ttttccgatc ttcttctccg atttagatct        120

cctctacatt gttgcttaat ctcagaacct tttttcgttg ttcctggatc tgaatgtgtt        180

tgtttgcaat ttcacgatct taaaaggtta gatctcgatt ggtattgacg attggaatct        240

ttacgatttc aggatgttta tttgcgttgt cctctgcaat agaagaggct acgaagttaa        300

ggatccttgg tgaaattaac aagccaatct gctaccaaaa cgatgataag attttctact        360

gtcctaagga ttgggttgga tacaacaacg tttgttacta ctttggcaac gaggaaaaga        420

actataacaa cgctagtaac tactgcaagc agctgaactc gacattgacg aacaacaaca        480

ctatcttggt gaacttaact aagaccctca acctcacaaa gacctacaac catgagtcta        540

actactgggt caactactca ctaattaaga acgagtctgt gcttcttagg gacagcgggt        600

actacaagaa gcagaagcac gtttccttgc tttacatatg ctcaaagccc cgggcagttg        660

gaagaccatg ccctatatgt cctgcttgtg aaggtccagg tccctctgct tttatattcc        720

caccaaagcc gaaggatacc ttgatgattt cacgtacacc acaagttact tgtgttgttg        780

tggatgtttc acaagaaaat cctgaggtac aattcagctg gtatgttgat ggggtagaag        840

tgcacactgc acagactcga ccaaaggagg cccagtttaa ctcgacttat agagttgttt        900

ctgttctccc aatccaacac gaagattggc tgaagggcaa ggaatttgaa tgcaaggtta        960

acaataaaga tctaccagca ccaattacca ggattatttc taaggcaaaa ggaccctcca       1020

gagagcccca agtttacaca ttgtctcctt ctgctgagga gcttagtaga agtaaagtga       1080

gcattacctg cttagtgacg ggattctacc ctccagacat cgacgtcgaa tggaaatcta       1140

atggtcaacc tgagccagaa ggtaactata ggactactcc accacaacag gacgtcgatg       1200

gcacatactt tctttattca aaacttgctg tcgataaggc aagttggcaa agaggagatc       1260

catttcagtg tgctgtaatg catgaggctt tgcataatca ttatacacag aaatcagttt       1320

ctaaaacaca agggaaacac gatgagctct agctcgag                               1358


<210>    11
<211>    2006
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    DNA sequence of full EP402 construct


<400>    11
tctagaatta ttacatcaaa acaaaaaatg gctcgctcgt ttggagctaa cagtaccgtt         60

gtgttggcga tcatcttctt cggtgagtga ttttccgatc ttcttctccg atttagatct        120

cctctacatt gttgcttaat ctcagaacct tttttcgttg ttcctggatc tgaatgtgtt        180

tgtttgcaat ttcacgatct taaaaggtta gatctcgatt ggtattgacg attggaatct        240

ttacgatttc aggatgttta tttgcgttgt cctctgcaat agaagaggct acgaagttaa        300

ggatcctttc taacattgtt cttagtattg attactgggt ttcttttaat aagacaatca        360

tcctcgattc taacattact aacgacaata acgatattaa tggagtgagt tggaactttt        420

ttaacaacag ctttaatact ctagcaacat gcggtaaagc tggcaatttt tgcgagtgca        480

gcaattactc aacaagtata tacaatatca ccaataattg ctcccttaca atatttcctc        540

ataacgatgt tttcgatact acatatcagg tcgtgtggaa tcagattatc aactacacga        600

tcaagctttt gacccctgct actcctccta acataaccta caactgtact aattttctga        660

taacgtgtaa gaagaataac ggtacaaaca ccaacattta tctgaatata aatgacacgt        720

tcgtgaaata cactaacgaa agtatccttg agtataactg gaataactcc aatattaata        780

acttcactgc aacttgtatc attaacaata ctatttcaac ttcgaacgaa acaaccttga        840

taaattgtac gtacttaact ctttcttcca attacttcta taccttcttt aagggaggag        900

ggggttcacg taaaagaaaa aagcacgttg aggaaattga gtcaccacct ccagaatcta        960

atgaggaaga acaatgtcag catgacgata ctacttctat tcacgaaccg tctcctaggg       1020

agcccctgct ccctaaacct tattcaagat atcagtacaa cactcctatt tactatatga       1080

gaccttcgac ccaaccactt aaccctttcc cccttcctaa accttgccct ccacctaagc       1140

catgccctcc tccgaagccc tgtccgcctc ctaagccctg tccaagtgcc gagtcttact       1200

ctcctcctaa gcctcttccg agcatccctc ttcttcccaa cattcctccg ctctccacac       1260

aaaatattag ccttatacat gtagatagga ttatcccccg ggcagttgga agaccatgcc       1320

ctatatgtcc tgcttgtgaa ggtccaggtc cctctgcttt tatattccca ccaaagccga       1380

aggatacctt gatgatttca cgtacaccac aagttacttg tgttgttgtg gatgtttcac       1440

aagaaaatcc tgaggtacaa ttcagctggt atgttgatgg ggtagaagtg cacactgcac       1500

agactcgacc aaaggaggcc cagtttaact cgacttatag agttgtttct gttctcccaa       1560

tccaacacga agattggctg aagggcaagg aatttgaatg caaggttaac aataaagatc       1620

taccagcacc aattaccagg attatttcta aggcaaaagg accctccaga gagccccaag       1680

tttacacatt gtctccttct gctgaggagc ttagtagaag taaagtgagc attacctgct       1740

tagtgacggg attctaccct ccagacatcg acgtcgaatg gaaatctaat ggtcaacctg       1800

agccagaagg taactatagg actactccac cacaacagga cgtcgatggc acatactttc       1860

tttattcaaa acttgctgtc gataaggcaa gttggcaaag aggagatcca tttcagtgtg       1920

ctgtaatgca tgaggctttg cataatcatt atacacagaa atcagtttct aaaacacaag       1980

ggaaacacga tgagctctag ctcgag                                            2006
