               SEQUENCE LISTING

<110> Glycom A/S

<120> NUCLEIC ACID CONSTRUCT

<130> 141WO00

<150> DK 2019 00756
<151> 2019-06-21

<160> 52

<170> BiSSAP 1.3.6

<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> 23 nb 5'UTR-glpF (the pin structure)

<400> 1
cgtggaggtc cgtgactttc acg                                            23


<210> 2
<211> 54
<212> DNA
<213> Artificial Sequence


<220> 
<223> 54nb 5'UTR-glpF

<400> 2
tgcctacaag catcgtggag gtccgtgact ttcacgcata caacaaacat taac           54


<210> 3
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Rec RBS (!6UTR)

<400> 3
caaggaggaa acagct                                                    16


<210> 4
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v1 (SD1)

<400> 4
caaattcgaa acagct                                                    16


<210> 5
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v2 (SD2)

<400> 5
caagcgcaaa acagct                                                    16


<210> 6
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v3 (SD3)

<400> 6
caagaacaaa acagct                                                    16


<210> 7
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v4 (SD4)

<400> 7
caactaggaa acagct                                                    16


<210> 8
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v5 (SD5)

<400> 8
caaccgagaa acagct                                                    16


<210> 9
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v6 (SD6)

<400> 9
caagagctaa acagct                                                    16


<210> 10
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v7 (SD7)

<400> 10
caagagcaaa acagct                                                    16


<210> 11
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v8 (SD8)

<400> 11
caagagaaaa acagct                                                    16


<210> 12
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v9 (SD9)

<400> 12
caaaggaaaa acagct                                                    16


<210> 13
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> recRBS_v10 (SD10)

<400> 13
caactgagaa acagct                                                    16


<210> 14
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16 nb lacZ RBS

<400> 14
cacacaggaa acagct                                                    16


<210> 15
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16 nb glpF RBS

<400> 15
tcttcaggat ccgatt                                                    16


<210> 16
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16 nb glpA RBS

<400> 16
cttcagaggg ataaca                                                    16


<210> 17
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16 nb glpA RBS

<400> 17
gccacggagg ctatca                                                    16


<210> 18
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> glpD RBS

<400> 18
agtgaatgag ggcagc                                                    16


<210> 19
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16 nb gatY RBS

<400> 19
acgacaggat atgaaa                                                    16


<210> 20
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> 16 nb mglB RBS

<400> 20
aaaaaccgga gatacc                                                    16


<210> 21
<211> 250
<212> DNA
<213> Artificial Sequence


<220> 
<223> PgatY_org

<400> 21
cggcaaccta tgcctgatgc gacgctgaag cgtcttatca tgcctacata gcactgccac     60

gtatgtttac accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg    120

gaggccgttt ttttgccttt cactcctcga ataattttca tattgtcgtt tttgtgatcg    180

ttatctcgat atttaaaaac aaataatttc attatatttt gaaatcgaaa acaaacgaca    240

ggatatgaaa                                                           250


<210> 22
<211> 350
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_org

<400> 22
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc      60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc     120

tatgattgca ccgttttaac gttgtaaccc gtatgtaaca gtgaataatc acttttgccg     180

aggtaacagc gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag     240

aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg     300

ccgagcattt atctcaagca ctaccctgca taagaaaaac cggagatacc                350


<210> 23
<211> 291
<212> DNA
<213> Artificial Sequence


<220> 
<223> PgatY_54UTR

<400> 23
cggcaaccta tgcctgatgc gacgctgaag cgtcttatca tgcctacata gcactgccac     60

gtatgtttac accgcatccg gcataaaaac acgcgcactt tgctacggct tccctatcgg    120

gaggccgttt ttttgccttt cactcctcga ataattttca tattgtcgtt tttgtgatcg    180

ttatctcgat atttaaaaac aaataatttc attatatttt gtgcctacaa gcatcgtgga    240

ggtccgtgac tttcacgcat acaacaaaca ttaacacgac aggatatgaa a             291


<210> 24
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_54UTR

<400> 24
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaacaaa aaccggagat acc                                            203


<210> 25
<211> 350
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_16UTR

<400> 25
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc      60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc     120

tatgattgca ccgttttaac gttgtaaccc gtatgtaaca gtgaataatc acttttgccg     180

aggtaacagc gtcataacaa caattaaagc cgttttctgg agcgttaccg ggcatggaag     240

aacgaatttt aaaaagtgag cttcggcgtt cagtaacact tcattaactc tactgccccg     300

ccgagcattt atctcaagca ctaccctgca taagcaagga ggaaacagct                350


<210> 26
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_70UTR

<400> 26
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa ggaggaaaca gct                                            203


<210> 27
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_70UTR_SD4

<400> 27
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa ctaggaaaca gct                                            203


<210> 28
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_70UTR_SD5

<400> 28
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa ccgagaaaca gct                                            203


<210> 29
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_70UTR_SD7

<400> 29
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa gagcaaaaca gct                                            203


<210> 30
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_70UTR_SD8

<400> 30
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa gagaaaaaca gct                                            203


<210> 31
<211> 203
<212> DNA
<213> Artificial Sequence


<220> 
<223> PmglB_70UTR_SD9

<400> 31
tgcgtcgcca ttctgtcgca acacgccaga atgcggcggc gatcactaac tcaacaaatc     60

aggcgatgta accgctttca atctgtgagt gatttcacag tatcttaaca atgtgatagc    120

tatgattgca ccgtgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa    180

cattaaccaa aggaaaaaca gct                                            203


<210> 32
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of plasmid backbone (forward)

<400> 32
attaacccuc caggcatcaa ataaaacgaa aggc                                 34


<210> 33
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of insert in galK site (forward)

<400> 33
cccagcgaga cctgaccgca gaac                                           24


<210> 34
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of insert in galK site (reverse)

<400> 34
ccccagtcca tcagcgtgac tacc                                           24


<210> 35
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of lacZ (forward)

<400> 35
aaacagcuat gaccatgatt acggattc                                       28


<210> 36
<211> 40
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of lacZ (reverse)

<400> 36
agggttaaut gcgcgttatt tttgacacca gaccaactgg                           40


<210> 37
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of plasmid backbone (reverse)

<400> 37
atttgcgcau caccaatcaa attcacgcgg cc                                   32


<210> 38
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PgatY-promoter elements (forward)

<400> 38
atgcgcaaau cggcaaccta tgcctgatgc gacgc                                35


<210> 39
<211> 37
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB-promoter elements (forward)

<400> 39
atgcgcaaau tgcgtcgcca ttctgtcgca acacgcc                              37


<210> 40
<211> 45
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_16UTR promoter element (reverse)

<400> 40
agctgttucc tccttgctta tgcagggtag tgcttgagat aaatg                     45


<210> 41
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_70UTR_SD4 promoter element
      (reverse)

<400> 41
agctgttucc tagttggtta atgtttgttg tatgcg                               36


<210> 42
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_70UTR_SD5 promoter element
      (reverse)

<400> 42
agctgttuct cggttggtta atgtttgttg tatgcg                               36


<210> 43
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_70UTR_SD7 promoter element
      (reverse)

<400> 43
agctgttutg ctcttggtta atgtttgttg tatgcg                               36


<210> 44
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_70UTR_SD8 promoter element
      (reverse)

<400> 44
agctgttutt ctcttggtta atgtttgttg tatgcg                               36


<210> 45
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_70UTR_SD9 promoter element
      (reverse)

<400> 45
agctgttutt cctttggtta atgtttgttg tatgcg                               36


<210> 46
<211> 95
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_70UTR promoter element (reverse)

<400> 46
agctgttucc tccttggtta atgtttgttg tatgcgtgaa agtcacggac ctccacgatg     60

cttgtaggca cggtgcaatc atagctatca cattg                                95


<210> 47
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_org promoter element (reverse)

<400> 47
atggtcaugg tatctccggt ttttcttatg caggg                                35


<210> 48
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PgatY_org promoter element (reverse)

<400> 48
atggtcautt tcatatcctg tcgtttgttt tcg                                  33


<210> 49
<211> 103
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PmglB_54UTR promoter element (reverse)

<400> 49
atggtcaugg tatctccggt ttttgttaat gtttgttgta tgcgtgaaag tcacggacct     60

ccacgatgct tgtaggcacg gtgcaatcat agctatcaca ttg                      103


<210> 50
<211> 100
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo for amplification of PgatY_54UTR promoter element (reverse)

<400> 50
atggtcautt tcatatcctg tcgtgttaat gtttgttgta tgcgtgaaag tcacggacct     60

ccacgatgct tgtaggcaca aaatataatg aaattatttg                          100


<210> 51
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> CRP__gatY

<400> 51
ttttgtgatc gttatctcga ta                                             22


<210> 52
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> CRP__mglB

<400> 52
atctgtgagt gatttcacag ta                                             22


