                               SEQUENCE LISTING

<110> THE SCRIPPS RESEARCH INSTITUTE
 
<120> REAGENTS AND METHODS FOR REPLICATION, TRANSCRIPTION, AND 
      TRANSLATION IN SEMI-SYNTHETIC ORGANISMS

<130> 36271-808.601

<140>
<141>

<150> 62/861,901
<151> 2019-06-14

<160> 20     

<170> PatentIn version 3.5

<210> 1
<211> 5923
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 1
gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt       60

gcgccgacat cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat      120

cggctcgtat aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagtattc      180

atggataaaa aaccgctgga cgttctgatc tccgctacgg gtctgtggat gagccgcacg      240

ggtacgctgc ataaaattaa acaccacgaa gtgtcacgtt cgaaaatcta tatcgaaatg      300

gcgtgcggtg atcatctggt ggttaacaat agccgttctt gtcgcaccgc gcgtgccttt      360

cgccatcaca aataccgcaa aacgtgcaaa cgttgtcgcg tgtcagatga agacattaac      420

aatttcctga cccgtagtac ggaatccaaa aactcagtga aagttcgcgt cgtgagtgct      480

ccgaaagtta aaaaagcgat gccgaaaagt gtctcccgtg ccccgaaacc gctggaaaac      540

tcagtgtcgg caaaagcttc caccaatacg agccgctctg ttccgtcgcc ggcaaaaagc      600

accccgaaca gctctgtccc ggcaagcgca ccggcaccgt ctctgacgcg tagtcagctg      660

gatcgcgtgg aagccctgct gtccccggaa gacaaaatct cactgaatat ggcaaaaccg      720

tttcgtgaac tggaaccgga actggttacc cgtcgcaaaa acgatttcca acgtctgtat      780

acgaatgatc gcgaagacta cctgggtaaa ctggaacgtg atatcaccaa atttttcgtg      840

gaccgcggct ttctggaaat caaatctccg attctgatcc cggctgaata tgttgaacgc      900

atgggtatta acaatgatac cgaactgagt aaacagattt ttcgtgtgga taaaaacctg      960

tgcctgcggc cgatgctggc accgacgctg tataattacc tgcgtaaact ggatcgcatt     1020

ctgccgggtc cgattaaaat ctttgaagtg ggcccgtgtt atcgtaaaga atcggatggc     1080

aaagaacacc tggaagaatt taccatggtt aacttctgcc aaatgggcag cggttgtacg     1140

cgcgaaaatc tggaagcgct gatcaaagaa ttcctggatt acctggaaat cgacttcgaa     1200

atcgtcggtg attcttgcat ggtgtatggc gataccctgg acatcatgca tggtgacctg     1260

gaactgagtt ccgctgttgt cggtccggtc agcctggatc gtgaatgggg cattgacaaa     1320

ccgtggatcg gcgcgggttt tggcctggaa cgcctgctga aagttatgca cggcttcaaa     1380

aacatcaaac gtgcgtctcg ctcggaatcg tattacaacg gcatctcaac caatctgtaa     1440

taatgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg     1500

cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt     1560

cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc     1620

gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag     1680

gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg     1740

cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga     1800

caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat     1860

ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca     1920

gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc     1980

gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca     2040

atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg     2100

caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca     2160

gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata     2220

accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag     2280

ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg     2340

gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca     2400

acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta     2460

atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct     2520

ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca     2580

gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag     2640

gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat     2700

tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt     2760

taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa     2820

cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga     2880

gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg     2940

gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc     3000

agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag     3060

aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc     3120

agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg     3180

cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac     3240

accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga     3300

aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt     3360

ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag     3420

cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg     3480

gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta     3540

tccccgagct cttagcgcga attgtcgagg gaaatttttt ctaaatacat tcaaatatgt     3600

atccgctcat gagacaataa ccctgataaa tgcttcaata atattaaata tggctggttc     3660

tcgcagaaag aaacatatcc atgaaatccc gccccgaatt gatatgtcca gattagataa     3720

aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac     3780

aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa     3840

aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca     3900

cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt     3960

tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac     4020

agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc     4080

actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt     4140

ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat     4200

gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt     4260

cttattcggc cttgaattga tcatttgcgg attagaaaaa caacttaaat gtgaaagtgg     4320

gtcttaagca ctaggtctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca     4380

aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc     4440

tctagcacgc gtagagctag agccttcaac ccagtcagct ccttccggtg ggcgcggggc     4500

atgactaaca tgagaattac aacttatatc gtatggggct gacttcaggt gctacatttg     4560

aagagataaa ttgcactgaa atctagatga ttctgtggat aaccgtatta ccgcctttga     4620

gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga     4680

agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg     4740

cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc     4800

ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga     4860

gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc     4920

tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg     4980

cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct     5040

ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg     5100

tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt     5160

gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca     5220

ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc     5280

tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt     5340

ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc     5400

tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca     5460

gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca     5520

aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct     5580

gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt     5640

gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca     5700

ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca     5760

ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc     5820

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg     5880

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aat                       5923


<210> 2
<211> 3101
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 2
taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga       60

ggaactatat ccggattggt taatacgact cactataggg gaattgtgag cggataacaa      120

ttcccctcta gaaaagcatt ggaaaccgag accggtaccg gtctcttaga ttcccggggt      180

ttccgccaaa ttcgaaaagc ctgctcaacg agcaggcttt tttgcatcta gcataacccc      240

ttggggcctc taaacgggtc ttgaggggtt ttttgcctga acgagcaggc ttttttgcat      300

aagcttccta gtggcagcgg ctaactaagc ggcctgctga ctttctcgcc gatcaaaagg      360

cattttgcta ttaagggatt gacgagggcg tatctgcgca gtaagatgcg ccccgcattg      420

gagacgccat ggcgtctcgg ttttagagct agaaatagca agttaaaata aggctagtcc      480

gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttaattcgaa aagcctgctc      540

aacgagcagg cttttttggt cgacagtagt ggcagcggct aactaagcgg cctgctgact      600

ttctcgccga tcaaaaggca ttttgctatt aagggattga cgagggcgta tctgcgcagt      660

aagatgcgcc ccgcatgaga cggcatgccg tctctagagc tagaaatagc aagttaaaat      720

aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaattcga      780

aaagcctgct caacgagcag gcttttttgg tcgacagttc ataggtgatt gcggatcccg      840

tcgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga      900

actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg      960

gagcggtcga gttctggacc gaccggctcg ggttctcccg cgacttcgtg gaggacgact     1020

tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg     1080

tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt     1140

ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg     1200

gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact     1260

tcgtggccga ggagcaggac tgagagctcg cttggactcc tgttgataga tccagtaatg     1320

acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt     1380

ggtgagaatc caagcactag ctagtaacaa cttatatcgt atggggctga cttcaggtgc     1440

tacatttgaa gagataaatt gcactgaaat ctagtaatat tttatctgat taataagatg     1500

atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc     1560

cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg     1620

gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga     1680

cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat     1740

gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa     1800

cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca     1860

ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca     1920

ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct     1980

gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc aggggggcgg     2040

agcctatgga aaaacggctt tgccgcggcc ctctcacttc cctgttaagt atcttcctgg     2100

catcttccag gaaatctccg ccccgttcgt aagccatttc cgctcgccgc agtcgaacga     2160

ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cccttaatac gactcactat     2220

agggtcccta tcagtgatag agaggtctag aaataatttt gtttaacttt aagaaggaga     2280

tatacatatg tcgaaaggcg aagaactgtt tacgggagtg gtgcctatcc tggtagagct     2340

cgacggagat gtaaacggtc acaaattttc agtccgcggg gaaggcgaag gcgatgcgac     2400

caacggtaaa ttaactttga agtttatttg caccaccggc aaattaccgg tgccttggcc     2460

gacgcttgtg acgaccctga cttacggggt gcagtgtttc agtcgctacc cagatcacat     2520

gaaacgccat gacttcttca aatctgcgat gccggaaggc tatgtgcagg aacgtacaat     2580

tagctttaaa gacgacggca cgtataaaac gcgggcagag gttaaatttg agggagatac     2640

cctggtaaac cgtattgaac tgaaaggcat cgattttaaa gaagatggga acatcttggg     2700

ccacaagaga ccggtaccgg tctcggaatc aaagcaaatt tcaagatccg tcataacgtg     2760

gaggacggtt ccgtgcagct tgcagatcac tatcagcaga atacgccgat tggcgatggc     2820

ccggtgctgc tgcccgataa tcactacctc tctactcaga gtgttttatc gaaagacccg     2880

aacgagaagc gtgatcacat ggtgctgctt gaatttgtta ccgcggcagg tattacacac     2940

ggcatggatg agttgtataa gggatccgct tggagccacc cgcagttcga gaaaggtgga     3000

ggttccggag gtggatcggg aggttcggcg tggagccacc cgcagttcga aaaataaaag     3060

cttaattagc tgagcttgga ctccctgcca ccgctgagca a                         3101


<210> 3
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
ctcgagtaca actttaactc acacaatgta tagatcacgg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 4
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 4
ctcgagtaca actttaactc acacaatgta tacatcacgg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 5
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (32)..(32)
<223> An unnatural nucleotide

<400> 5
ctcgagtaca actttaactc acacaatgta ancatcacgg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 6
<211> 52
<212> DNA
<213> Methanosarcina mazei

<400> 6
gaatctaacc cggctgaacg gatttagagt ccgttcgatc tacatgatca gg               52


<210> 7
<211> 52
<212> DNA
<213> Methanosarcina mazei

<400> 7
gaatctaacc cggctgaacg gatttacagt ccgttcgatc tacatgatca gg               52


<210> 8
<211> 52
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(26)
<223> An unnatural nucleotide

<400> 8
gaatctaacc cggctgaacg gattancagt ccgttcgatc tacatgatca gg               52


<210> 9
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(26)
<223> An unnatural nucleotide

<400> 9
ctcgagtaca actttaactc acacancgta tacatcacgg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 10
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (38)..(38)
<223> An unnatural nucleotide

<400> 10
ctcgagtaca actttaactc acacaatgta tacatcancg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 11
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(26)
<223> An unnatural nucleotide

<220>
<221> modified_base
<222> (32)..(32)
<223> An unnatural nucleotide

<400> 11
ctcgagtaca actttaactc acacancgta ancatcacgg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 12
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (32)..(32)
<223> An unnatural nucleotide

<220>
<221> modified_base
<222> (38)..(38)
<223> An unnatural nucleotide

<400> 12
ctcgagtaca actttaactc acacaatgta ancatcancg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 13
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220>
<221> modified_base
<222> (26)..(26)
<223> An unnatural nucleotide

<220>
<221> modified_base
<222> (38)..(38)
<223> An unnatural nucleotide

<400> 13
ctcgagtaca actttaactc acacancgta tacatcancg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 14
<211> 63
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide 


<220>
<221> modified_base
<222> (26)..(26)
<223> An unnatural nucleotide

<220>
<221> modified_base
<222> (32)..(32)
<223> An unnatural nucleotide

<220>
<221> modified_base
<222> (38)..(38)
<223> An unnatural nucleotide

<400> 14
ctcgagtaca actttaactc acacancgta ancatcancg cagacaaaca aaagaatgga       60

atc                                                                     63


<210> 15
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
atgggtctca cacaaactcg agtacaactt taactcacac                             40


<210> 16
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
atgggtctcg attccattct tttgtttgtc tgc                                    33


<210> 17
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 17
atgggtctcg aaacctgatc atgtagatcg aacgg                                  35


<210> 18
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 18
atgggtctca tctaacccgg ctgaacgg                                          28


<210> 19
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 19
ctcgagtaca actttaactc acac                                              24


<210> 20
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 20
gattccattc ttttgtttgt ctgc                                              24



