﻿               SEQUENCE LISTING

<110> Uniwersytet Gdanski

<120> Sekwencje aminokwasowe rekombinowanych białek flawiwirusowych oraz rekombinowanych flawiwirusowych cząstek wirusopodobnych, ich zastosowanie do zapobiegania zakażeniom wirusem Zika, oraz ich zastosowanie diagnostyczne in vitro

<130> 1

<160> 11

<170> BiSSAP 1.3.6

<210> 1
<211> 184
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16
<223> SEQ 11

<220> 
<223> optssprM

<400> 1
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      
Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          
Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              
Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  
Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  
Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      
Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         
Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             
Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 
Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 
Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     
Leu Leu Ile Ala Pro Ala Tyr Ser 
            180                 

<210> 2
<211> 522
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16
<223> SEQ 11

<220> 
<223> optssE

<400> 2
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
            20                  25                  30          
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
        35                  40                  45              
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
    50                  55                  60                  
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
65                  70                  75                  80  
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
                85                  90                  95      
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
            100                 105                 110         
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
        115                 120                 125             
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
    130                 135                 140                 
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
145                 150                 155                 160 
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
                165                 170                 175     
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
            180                 185                 190         
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
        195                 200                 205             
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
    210                 215                 220                 
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
225                 230                 235                 240 
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
                245                 250                 255     
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
            260                 265                 270         
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
        275                 280                 285             
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
    290                 295                 300                 
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
305                 310                 315                 320 
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
                325                 330                 335     
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
            340                 345                 350         
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
        355                 360                 365             
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
    370                 375                 380                 
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
385                 390                 395                 400 
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
                405                 410                 415     
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
            420                 425                 430         
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
        435                 440                 445             
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
    450                 455                 460                 
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
465                 470                 475                 480 
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
                485                 490                 495     
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
            500                 505                 510         
Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
        515                 520         

<210> 3
<211> 522
<212> PRT
<213> Artificial Sequence


<220> 
<223> ssEchimera

<220> 
<221> DOMAIN
<222> 426..522
<223> domeny helikalne i transmembranowe białka E wirusa TBEV

<400> 3
Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
1               5                   10                  15      
Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
            20                  25                  30          
Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
        35                  40                  45              
Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
    50                  55                  60                  
Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
65                  70                  75                  80  
Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
                85                  90                  95      
Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
            100                 105                 110         
Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
        115                 120                 125             
Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
    130                 135                 140                 
Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
145                 150                 155                 160 
Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
                165                 170                 175     
Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
            180                 185                 190         
Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
        195                 200                 205             
Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
    210                 215                 220                 
Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
225                 230                 235                 240 
Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
                245                 250                 255     
Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
            260                 265                 270         
Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
        275                 280                 285             
Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
    290                 295                 300                 
Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
305                 310                 315                 320 
Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
                325                 330                 335     
Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
            340                 345                 350         
Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
        355                 360                 365             
Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
    370                 375                 380                 
Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
385                 390                 395                 400 
Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
                405                 410                 415     
His Trp His Arg Ser Gly Ser Thr Ile Gly Arg Val Phe Gln Lys Thr 
            420                 425                 430         
Lys Lys Gly Ile Glu Arg Leu Thr Val Ile Gly Glu His Ala Trp Asp 
        435                 440                 445             
Phe Gly Ser Ala Gly Gly Phe Leu Ser Ser Ile Gly Lys Ala Val His 
    450                 455                 460                 
Thr Val Leu Gly Gly Ala Phe Asn Ser Ile Phe Gly Gly Val Gly Phe 
465                 470                 475                 480 
Leu Pro Lys Leu Leu Leu Gly Val Ala Leu Ala Trp Leu Gly Leu Asn 
                485                 490                 495     
Met Arg Asn Pro Thr Met Ser Met Ser Phe Leu Leu Ala Gly Gly Leu 
            500                 505                 510         
Val Leu Ala Met Thr Leu Gly Val Gly Ala 
        515                 520         

<210> 4
<211> 520
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16
<223> SEQ 11

<220> 
<223> optssEchimera

<220> 
<221> DOMAIN
<222> 424..520
<223> TBEV stem/anchor

<400> 4
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
            20                  25                  30          
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
        35                  40                  45              
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
    50                  55                  60                  
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
65                  70                  75                  80  
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
                85                  90                  95      
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
            100                 105                 110         
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
        115                 120                 125             
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
    130                 135                 140                 
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
145                 150                 155                 160 
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
                165                 170                 175     
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
            180                 185                 190         
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
        195                 200                 205             
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
    210                 215                 220                 
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
225                 230                 235                 240 
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
                245                 250                 255     
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
            260                 265                 270         
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
        275                 280                 285             
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
    290                 295                 300                 
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
305                 310                 315                 320 
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
                325                 330                 335     
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
            340                 345                 350         
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
        355                 360                 365             
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
    370                 375                 380                 
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
385                 390                 395                 400 
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
                405                 410                 415     
His Arg Ser Gly Ser Thr Ile Gly Arg Val Phe Gln Lys Thr Lys Lys 
            420                 425                 430         
Gly Ile Glu Arg Leu Thr Val Ile Gly Glu His Ala Trp Asp Phe Gly 
        435                 440                 445             
Ser Ala Gly Gly Phe Leu Ser Ser Ile Gly Lys Ala Val His Thr Val 
    450                 455                 460                 
Leu Gly Gly Ala Phe Asn Ser Ile Phe Gly Gly Val Gly Phe Leu Pro 
465                 470                 475                 480 
Lys Leu Leu Leu Gly Val Ala Leu Ala Trp Leu Gly Leu Asn Met Arg 
                485                 490                 495     
Asn Pro Thr Met Ser Met Ser Phe Leu Leu Ala Gly Gly Leu Val Leu 
            500                 505                 510         
Ala Met Thr Leu Gly Val Gly Ala 
        515                 520 

<210> 5
<211> 690
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16
<223> SEQ 11

<220> 
<223> optssprME

<400> 5
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      
Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          
Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              
Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  
Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  
Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      
Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         
Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             
Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 
Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 
Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     
Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         
Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             
Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 
Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 
Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     
Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         
Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             
Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 
Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 
Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     
Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         
Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             
Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 
Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 
Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     
Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         
His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             
Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 
Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 
Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     
Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         
Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             
Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 
Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 
Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     
Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         
Lys Ala Phe Glu Ala Thr Val Arg Gly Ala Lys Arg Met Ala Val Leu 
        595                 600                 605             
Gly Asp Thr Ala Trp Asp Phe Gly Ser Val Gly Gly Ala Leu Asn Ser 
    610                 615                 620                 
Leu Gly Lys Gly Ile His Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu 
625                 630                 635                 640 
Phe Gly Gly Met Ser Trp Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu 
                645                 650                 655     
Met Trp Leu Gly Leu Asn Thr Lys Asn Gly Ser Ile Ser Leu Met Cys 
            660                 665                 670         
Leu Ala Leu Gly Gly Val Leu Ile Phe Leu Ser Thr Ala Val Ser Ala 
        675                 680                 685             
Asp Val 
    690 

<210> 6
<211> 688
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16
<223> SEQ 11

<220> 
<223> optssprMEchimera

<220> 
<221> DOMAIN
<222> 592..688
<223> TBEV stem/anchor

<400> 6
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      
Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          
Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              
Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  
Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  
Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      
Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         
Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             
Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 
Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 
Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     
Leu Leu Ile Ala Pro Ala Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn 
            180                 185                 190         
Arg Asp Phe Val Glu Gly Met Ser Gly Gly Thr Trp Val Asp Val Val 
        195                 200                 205             
Leu Glu His Gly Gly Cys Val Thr Val Met Ala Gln Asp Lys Pro Thr 
    210                 215                 220                 
Val Asp Ile Glu Leu Val Thr Thr Thr Val Ser Asn Met Ala Glu Val 
225                 230                 235                 240 
Arg Ser Tyr Cys Tyr Glu Ala Ser Ile Ser Asp Met Ala Ser Asp Ser 
                245                 250                 255     
Arg Cys Pro Thr Gln Gly Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr 
            260                 265                 270         
Gln Tyr Val Cys Lys Arg Thr Leu Val Asp Arg Gly Trp Gly Asn Gly 
        275                 280                 285             
Cys Gly Leu Phe Gly Lys Gly Ser Leu Val Thr Cys Ala Lys Phe Ala 
    290                 295                 300                 
Cys Ser Lys Lys Met Thr Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu 
305                 310                 315                 320 
Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His Ser Gly Met Ile 
                325                 330                 335     
Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg Ala Lys Val Glu 
            340                 345                 350         
Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly 
        355                 360                 365             
Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp 
    370                 375                 380                 
Leu Tyr Tyr Leu Thr Met Asn Asn Lys His Trp Leu Val His Lys Glu 
385                 390                 395                 400 
Trp Phe His Asp Ile Pro Leu Pro Trp His Ala Gly Ala Asp Thr Gly 
                405                 410                 415     
Thr Pro His Trp Asn Asn Lys Glu Ala Leu Val Glu Phe Lys Asp Ala 
            420                 425                 430         
His Ala Lys Arg Gln Thr Val Val Val Leu Gly Ser Gln Glu Gly Ala 
        435                 440                 445             
Val His Thr Ala Leu Ala Gly Ala Leu Glu Ala Glu Met Asp Gly Ala 
    450                 455                 460                 
Lys Gly Arg Leu Ser Ser Gly His Leu Lys Cys Arg Leu Lys Met Asp 
465                 470                 475                 480 
Lys Leu Arg Leu Lys Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe 
                485                 490                 495     
Thr Phe Thr Lys Ile Pro Ala Glu Thr Leu His Gly Thr Val Thr Val 
            500                 505                 510         
Glu Val Gln Tyr Ala Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln 
        515                 520                 525             
Met Ala Val Asp Met Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr 
    530                 535                 540                 
Ala Asn Pro Val Ile Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu 
545                 550                 555                 560 
Glu Leu Asp Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly 
                565                 570                 575     
Glu Lys Lys Ile Thr His His Trp His Arg Ser Gly Ser Thr Ile Gly 
            580                 585                 590         
Arg Val Phe Gln Lys Thr Lys Lys Gly Ile Glu Arg Leu Thr Val Ile 
        595                 600                 605             
Gly Glu His Ala Trp Asp Phe Gly Ser Ala Gly Gly Phe Leu Ser Ser 
    610                 615                 620                 
Ile Gly Lys Ala Val His Thr Val Leu Gly Gly Ala Phe Asn Ser Ile 
625                 630                 635                 640 
Phe Gly Gly Val Gly Phe Leu Pro Lys Leu Leu Leu Gly Val Ala Leu 
                645                 650                 655     
Ala Trp Leu Gly Leu Asn Met Arg Asn Pro Thr Met Ser Met Ser Phe 
            660                 665                 670         
Leu Leu Ala Gly Gly Leu Val Leu Ala Met Thr Leu Gly Val Gly Ala 
        675                 680                 685             



<210> 7
<211> 732
<212> PRT
<213> Artificial Sequence


<220> 
<223> ss1prMP2AssE

<220> 
<221> SITE
<222> 187..189
<223> GSG

<220> 
<221> PEPTIDE
<222> 190..208
<223> P2A

<400> 7
Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      
Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          
Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              
Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  
Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  
Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      
Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         
Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             
Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 
Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 
Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     
Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Gly Ser Gly Ala Thr Asn 
            180                 185                 190         
Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
        195                 200                 205             
Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
    210                 215                 220                 
Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
225                 230                 235                 240 
Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
                245                 250                 255     
Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
            260                 265                 270         
Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
        275                 280                 285             
Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
    290                 295                 300                 
Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
305                 310                 315                 320 
Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
                325                 330                 335     
Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
            340                 345                 350         
Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
        355                 360                 365             
Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
    370                 375                 380                 
Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
385                 390                 395                 400 
Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
                405                 410                 415     
Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
            420                 425                 430         
Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
        435                 440                 445             
Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
    450                 455                 460                 
Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
465                 470                 475                 480 
Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
                485                 490                 495     
Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
            500                 505                 510         
Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
        515                 520                 525             
Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
    530                 535                 540                 
Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
545                 550                 555                 560 
Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
                565                 570                 575     
Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
            580                 585                 590         
Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
        595                 600                 605             
Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
    610                 615                 620                 
His Trp His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr 
625                 630                 635                 640 
Val Arg Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp 
                645                 650                 655     
Phe Gly Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His 
            660                 665                 670         
Gln Ile Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp 
        675                 680                 685             
Phe Ser Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn 
    690                 695                 700                 
Thr Lys Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val 
705                 710                 715                 720 
Leu Ile Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
                725                 730         

<210> 8
<211> 730
<212> PRT
<213> Artificial Sequence


<220> 
<223> ss1prMP2AssEchimera

<220> 
<221> SITE
<222> 187..189
<223> GSG

<220> 
<221> PEPTIDE
<222> 190..208
<223> P2A

<220> 
<221> DOMAIN
<222> 634..730
<223> TBEV stem/anchor

<400> 8
Gly Ala Asp Thr Ser Val Gly Ile Val Gly Leu Leu Leu Thr Thr Ala 
1               5                   10                  15      
Met Ala Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu 
            20                  25                  30          
Asp Arg Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly 
        35                  40                  45              
Met Asn Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp 
    50                  55                  60                  
Ala Thr Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro 
65                  70                  75                  80  
Asp Asp Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr 
                85                  90                  95      
Gly Thr Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala 
            100                 105                 110         
Val Thr Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln 
        115                 120                 125             
Thr Trp Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu 
    130                 135                 140                 
Asn Trp Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile 
145                 150                 155                 160 
Ala Trp Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val 
                165                 170                 175     
Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser Gly Ser Gly Ala Thr Asn 
            180                 185                 190         
Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro 
        195                 200                 205             
Ser Gln Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala 
    210                 215                 220                 
Tyr Ser Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly 
225                 230                 235                 240 
Met Ser Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys 
                245                 250                 255     
Val Thr Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val 
            260                 265                 270         
Thr Thr Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu 
        275                 280                 285             
Ala Ser Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly 
    290                 295                 300                 
Glu Ala Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg 
305                 310                 315                 320 
Thr Leu Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys 
                325                 330                 335     
Gly Ser Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr 
            340                 345                 350         
Gly Lys Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser 
        355                 360                 365             
Val His Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His 
    370                 375                 380                 
Glu Thr Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro 
385                 390                 395                 400 
Arg Ala Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys 
                405                 410                 415     
Glu Pro Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met 
            420                 425                 430         
Asn Asn Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro 
        435                 440                 445             
Leu Pro Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn 
    450                 455                 460                 
Lys Glu Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr 
465                 470                 475                 480 
Val Val Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala 
                485                 490                 495     
Gly Ala Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser 
            500                 505                 510         
Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
        515                 520                 525             
Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro 
    530                 535                 540                 
Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly 
545                 550                 555                 560 
Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln 
                565                 570                 575     
Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr 
            580                 585                 590         
Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe 
        595                 600                 605             
Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His 
    610                 615                 620                 
His Trp His Arg Ser Gly Ser Thr Ile Gly Arg Val Phe Gln Lys Thr 
625                 630                 635                 640 
Lys Lys Gly Ile Glu Arg Leu Thr Val Ile Gly Glu His Ala Trp Asp 
                645                 650                 655     
Phe Gly Ser Ala Gly Gly Phe Leu Ser Ser Ile Gly Lys Ala Val His 
            660                 665                 670         
Thr Val Leu Gly Gly Ala Phe Asn Ser Ile Phe Gly Gly Val Gly Phe 
        675                 680                 685             
Leu Pro Lys Leu Leu Leu Gly Val Ala Leu Ala Trp Leu Gly Leu Asn 
    690                 695                 700                 
Met Arg Asn Pro Thr Met Ser Met Ser Phe Leu Leu Ala Gly Gly Leu 
705                 710                 715                 720 
Val Leu Ala Met Thr Leu Gly Val Gly Ala 
                725                 730 

<210> 9
<211> 730
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16
<223> SEQ 11

<220> 
<223> optssprMP2AssE

<220> 
<221> SITE
<222> 185..187
<223> GSG

<220> 
<221> PEPTIDE
<222> 188..206
<223> P2A

<400> 9
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      
Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          
Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              
Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  
Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  
Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      
Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         
Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             
Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 
Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 
Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     
Leu Leu Ile Ala Pro Ala Tyr Ser Gly Ser Gly Ala Thr Asn Phe Ser 
            180                 185                 190         
Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Ser Gln 
        195                 200                 205             
Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
    210                 215                 220                 
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
225                 230                 235                 240 
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
                245                 250                 255     
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
            260                 265                 270         
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
        275                 280                 285             
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
    290                 295                 300                 
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
305                 310                 315                 320 
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
                325                 330                 335     
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
            340                 345                 350         
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
        355                 360                 365             
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
    370                 375                 380                 
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
385                 390                 395                 400 
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
                405                 410                 415     
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
            420                 425                 430         
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
        435                 440                 445             
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
    450                 455                 460                 
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
465                 470                 475                 480 
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
                485                 490                 495     
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
            500                 505                 510         
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
        515                 520                 525             
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
    530                 535                 540                 
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
545                 550                 555                 560 
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
                565                 570                 575     
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
            580                 585                 590         
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
        595                 600                 605             
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
    610                 615                 620                 
His Arg Ser Gly Ser Thr Ile Gly Lys Ala Phe Glu Ala Thr Val Arg 
625                 630                 635                 640 
Gly Ala Lys Arg Met Ala Val Leu Gly Asp Thr Ala Trp Asp Phe Gly 
                645                 650                 655     
Ser Val Gly Gly Ala Leu Asn Ser Leu Gly Lys Gly Ile His Gln Ile 
            660                 665                 670         
Phe Gly Ala Ala Phe Lys Ser Leu Phe Gly Gly Met Ser Trp Phe Ser 
        675                 680                 685             
Gln Ile Leu Ile Gly Thr Leu Leu Met Trp Leu Gly Leu Asn Thr Lys 
    690                 695                 700                 
Asn Gly Ser Ile Ser Leu Met Cys Leu Ala Leu Gly Gly Val Leu Ile 
705                 710                 715                 720 
Phe Leu Ser Thr Ala Val Ser Ala Asp Val 
                725                 730 

<210> 10
<211> 728
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16
<223> SEQ 11

<220> 
<223> optssprMP2AssEchimera

<220> 
<221> SITE
<222> 185..187
<223> GSG

<220> 
<221> PEPTIDE
<222> 188..206
<223> P2A

<220> 
<221> DOMAIN
<222> 632..728
<223> TBEV stem/anchor

<400> 10
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      
Ala Glu Val Thr Arg Arg Gly Ser Ala Tyr Tyr Met Tyr Leu Asp Arg 
            20                  25                  30          
Asn Asp Ala Gly Glu Ala Ile Ser Phe Pro Thr Thr Leu Gly Met Asn 
        35                  40                  45              
Lys Cys Tyr Ile Gln Ile Met Asp Leu Gly His Met Cys Asp Ala Thr 
    50                  55                  60                  
Met Ser Tyr Glu Cys Pro Met Leu Asp Glu Gly Val Glu Pro Asp Asp 
65                  70                  75                  80  
Val Asp Cys Trp Cys Asn Thr Thr Ser Thr Trp Val Val Tyr Gly Thr 
                85                  90                  95      
Cys His His Lys Lys Gly Glu Ala Arg Arg Ser Arg Arg Ala Val Thr 
            100                 105                 110         
Leu Pro Ser His Ser Thr Arg Lys Leu Gln Thr Arg Ser Gln Thr Trp 
        115                 120                 125             
Leu Glu Ser Arg Glu Tyr Thr Lys His Leu Ile Arg Val Glu Asn Trp 
    130                 135                 140                 
Ile Phe Arg Asn Pro Gly Phe Ala Leu Ala Ala Ala Ala Ile Ala Trp 
145                 150                 155                 160 
Leu Leu Gly Ser Ser Thr Ser Gln Lys Val Ile Tyr Leu Val Met Ile 
                165                 170                 175     
Leu Leu Ile Ala Pro Ala Tyr Ser Gly Ser Gly Ala Thr Asn Phe Ser 
            180                 185                 190         
Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn Pro Gly Pro Ser Gln 
        195                 200                 205             
Lys Val Ile Tyr Leu Val Met Ile Leu Leu Ile Ala Pro Ala Tyr Ser 
    210                 215                 220                 
Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
225                 230                 235                 240 
Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
                245                 250                 255     
Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
            260                 265                 270         
Thr Val Ser Asn Met Ala Glu Val Arg Ser Tyr Cys Tyr Glu Ala Ser 
        275                 280                 285             
Ile Ser Asp Met Ala Ser Asp Ser Arg Cys Pro Thr Gln Gly Glu Ala 
    290                 295                 300                 
Tyr Leu Asp Lys Gln Ser Asp Thr Gln Tyr Val Cys Lys Arg Thr Leu 
305                 310                 315                 320 
Val Asp Arg Gly Trp Gly Asn Gly Cys Gly Leu Phe Gly Lys Gly Ser 
                325                 330                 335     
Leu Val Thr Cys Ala Lys Phe Ala Cys Ser Lys Lys Met Thr Gly Lys 
            340                 345                 350         
Ser Ile Gln Pro Glu Asn Leu Glu Tyr Arg Ile Met Leu Ser Val His 
        355                 360                 365             
Gly Ser Gln His Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr 
    370                 375                 380                 
Asp Glu Asn Arg Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala 
385                 390                 395                 400 
Glu Ala Thr Leu Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro 
                405                 410                 415     
Arg Thr Gly Leu Asp Phe Ser Asp Leu Tyr Tyr Leu Thr Met Asn Asn 
            420                 425                 430         
Lys His Trp Leu Val His Lys Glu Trp Phe His Asp Ile Pro Leu Pro 
        435                 440                 445             
Trp His Ala Gly Ala Asp Thr Gly Thr Pro His Trp Asn Asn Lys Glu 
    450                 455                 460                 
Ala Leu Val Glu Phe Lys Asp Ala His Ala Lys Arg Gln Thr Val Val 
465                 470                 475                 480 
Val Leu Gly Ser Gln Glu Gly Ala Val His Thr Ala Leu Ala Gly Ala 
                485                 490                 495     
Leu Glu Ala Glu Met Asp Gly Ala Lys Gly Arg Leu Ser Ser Gly His 
            500                 505                 510         
Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly Val Ser 
        515                 520                 525             
Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu 
    530                 535                 540                 
Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp 
545                 550                 555                 560 
Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu 
                565                 570                 575     
Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser 
            580                 585                 590         
Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp 
        595                 600                 605             
Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp 
    610                 615                 620                 
His Arg Ser Gly Ser Thr Ile Gly Arg Val Phe Gln Lys Thr Lys Lys 
625                 630                 635                 640 
Gly Ile Glu Arg Leu Thr Val Ile Gly Glu His Ala Trp Asp Phe Gly 
                645                 650                 655     
Ser Ala Gly Gly Phe Leu Ser Ser Ile Gly Lys Ala Val His Thr Val 
            660                 665                 670         
Leu Gly Gly Ala Phe Asn Ser Ile Phe Gly Gly Val Gly Phe Leu Pro 
        675                 680                 685             
Lys Leu Leu Leu Gly Val Ala Leu Ala Trp Leu Gly Leu Asn Met Arg 
    690                 695                 700                 
Asn Pro Thr Met Ser Met Ser Phe Leu Leu Ala Gly Gly Leu Val Leu 
705                 710                 715                 720 
Ala Met Thr Leu Gly Val Gly Ala 
                725             

<210> 11
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<221> SIGNAL
<222> 1..16

<220> 
<223> optss

<400> 11
Met Val Gly Val Ala Leu Ala Leu Ala Leu Ala Thr Thr Ala Met Ala 
1               5                   10                  15      



