                         SEQUENCE LISTING

<110>  JUNO THERAPEUTICS INC
       Aifuwa, Ivie
       Beauchense, Pascal
       Dhuu-Duong, Kien
       Leuba, Kohana
 
<120>  Automated T Cell Culture

<130>  132777-255015-P001-PCT

<150>  62/858,736
<151>  2019-06-07

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  159
<212>  PRT
<213>  Streptomyces avidinii

<400>  1

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  2
<211>  126
<212>  PRT
<213>  Streptomyces avidinii

<400>  2

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser 
            20                  25                  30          


Ala Val Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Streptavidin-binding peptide

<400>  3

His Pro Gln Phe 
1               


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic streptavadin-binding peptide


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  W, K or R

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  G or E

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  G, K or R

<400>  4

Xaa Xaa His Pro Gln Phe Xaa Xaa 
1               5               


<210>  5
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Streptavidin-binding peptide


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  G or E

<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  G, K or R

<400>  5

Trp Xaa His Pro Gln Phe Xaa Xaa 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Streptavidin-binding peptide, Strep-tag

<400>  6

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  7
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223> Synthetic  Strep-tag II

<400>  7

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  8
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequential modules of streptavidin-binding peptide


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Any amino acid

<220>
<221>  REPEAT
<222>  (9)..(9)
<223>  Repeated 8 or 12 times

<400>  8

Trp Ser His Pro Gln Phe Glu Lys Xaa Trp Ser His Pro Gln Phe Glu 
1               5                   10                  15      


Lys 
    


<210>  9
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequential modules of streptavidin-binding peptide


<220>
<221>  REPEAT
<222>  (9)..(12)
<223>  Repeated two or three times

<400>  9

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Trp Ser His Pro 
1               5                   10                  15      


Gln Phe Glu Lys 
            20  


<210>  10
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Twin-Strep-peptide tag

<400>  10

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
            20                  25              


<210>  11
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Twin-Strep-peptide tag

<400>  11

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Trp Ser His Pro Gln Phe Glu Lys 
            20                  


<210>  12
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Twin-Strep-peptide tag

<400>  12

Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25              


<210>  13
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Twin-Strep-peptide tag

<400>  13

Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Ser Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  30  


<210>  14
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Twin-Strep-peptide tag

<400>  14

Ser Ala Trp Ser His Pro Gln Phe Glu Lys Gly Gly Gly Ser Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
            20                  25                  30  


<210>  15
<211>  159
<212>  PRT
<213>  Streptomyces avidinii

<400>  15

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu Ser Ala Val Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  16
<211>  126
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MUTAGEN
<222>  (31)..(34)

<400>  16

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val Thr 
            20                  25                  30          


Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  17
<211>  127
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MUTAGEN
<222>  (32)..(35)

<400>  17

Met Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr 
1               5                   10                  15      


Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val 
            20                  25                  30          


Thr Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr 
        35                  40                  45              


Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr 
    50                  55                  60                  


Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp 
65                  70                  75                  80  


Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp 
                85                  90                  95      


Leu Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu 
            100                 105                 110         


Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125         


<210>  18
<211>  159
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MUTAGEN
<222>  (44)..(47)

<400>  18

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Ile Gly Ala Arg Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser Ile Asp Ala Ala Lys 
    130                 135                 140                 


Lys Ala Gly Val Asn Asn Gly Asn Pro Leu Asp Ala Val Gln Gln 
145                 150                 155                 


<210>  19
<211>  126
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MUTAGEN
<222>  (34)..(37)

<400>  19

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Ile Gly 
            20                  25                  30          


Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  20
<211>  127
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MUTAGEN
<222>  (32)..(35)

<400>  20

Met Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr 
1               5                   10                  15      


Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Ile 
            20                  25                  30          


Gly Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr 
        35                  40                  45              


Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr 
    50                  55                  60                  


Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp 
65                  70                  75                  80  


Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp 
                85                  90                  95      


Leu Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu 
            100                 105                 110         


Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125         


<210>  21
<211>  126
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MUTAGEN
<222>  (31)..(34)

<400>  21

Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe 
1               5                   10                  15      


Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val Thr 
            20                  25                  30          


Ala Arg Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp 
        35                  40                  45              


Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val 
    50                  55                  60                  


Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser 
65                  70                  75                  80  


Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu 
                85                  90                  95      


Leu Thr Ser Gly Thr Thr Glu Glu Asn Ala Gly Tyr Ser Thr Leu Val 
            100                 105                 110         


Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125     


<210>  22
<211>  139
<212>  PRT
<213>  Streptomyces avidinii


<220>
<221>  MUTAGEN
<222>  (44)..(47)

<400>  22

Asp Pro Ser Lys Asp Ser Lys Ala Gln Val Ser Ala Ala Glu Ala Gly 
1               5                   10                  15      


Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr Phe Ile Val Thr 
            20                  25                  30          


Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Val Thr Ala Arg Gly 
        35                  40                  45              


Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr Asp Ser Ala Pro 
    50                  55                  60                  


Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr Val Ala Trp Lys 
65                  70                  75                  80  


Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp Ser Gly Gln Tyr 
                85                  90                  95      


Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Leu Leu Thr Ser 
            100                 105                 110         


Gly Thr Thr Glu Glu Asn Ala Gly Tyr Ser Thr Leu Val Gly His Asp 
        115                 120                 125             


Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
    130                 135                 


<210>  23
<211>  127
<212>  PRT
<213>  Streptomyces avidinii

<400>  23

Met Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln Leu Gly Ser Thr 
1               5                   10                  15      


Phe Ile Val Thr Ala Gly Ala Asp Gly Ala Leu Thr Gly Thr Tyr Glu 
            20                  25                  30          


Ser Ala Val Gly Asn Ala Glu Ser Arg Tyr Val Leu Thr Gly Arg Tyr 
        35                  40                  45              


Asp Ser Ala Pro Ala Thr Asp Gly Ser Gly Thr Ala Leu Gly Trp Thr 
    50                  55                  60                  


Val Ala Trp Lys Asn Asn Tyr Arg Asn Ala His Ser Ala Thr Thr Trp 
65                  70                  75                  80  


Ser Gly Gln Tyr Val Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp 
                85                  90                  95      


Leu Leu Thr Ser Gly Thr Thr Glu Ala Asn Ala Trp Lys Ser Thr Leu 
            100                 105                 110         


Val Gly His Asp Thr Phe Thr Lys Val Lys Pro Ser Ala Ala Ser 
        115                 120                 125         


