SEQUENCE LISTING

<110> FERTIS INDIA PVT. LTD
<120> Genetic modification of endophytic/epiphytic/rhizospheric microbes for Improved Nitrogen Fixation for Crops
<130>
<140> PCT/IN----/0-----
<141> PCT filing date:
<150> Indian Patent Application No.201841045699
<151>
<160> 16



<210> SEQ ID NO:1
<211> 1560
<212> DNA 
<213> Wild type 
<220>
<223> NifL:WT Gene encoding nitrogen fixation regulatory protein
<400> 1

atgtcagcgc ccgattccat gcaagcagtg tttgccgatg cagtgttcca cgatgcggtt 60
gagcaatcgg cgatcgcgat ctcgattacc gacggcaatg ctcacatcct ctatgtaaac 120
cccgcgttct gtgcaattac aggctatgcg ccgggcgaag tcgtcggcaa gcggcaatcg 180
ctgttgtctt acaagtcgac gcccagaagc gtgtatcaga agatgtggca agcgctcagg 240
gcgggacagt cctggaccgg gcggctgttg aaccggcgtc gggatggttc gccttatgtg 300
gccgaactca ccgtgacccc cttgcgttcg tcggtgagtc gggaggacga ggtgcgctac 360
ctgggcatgc actgggatgc caccgaggag catcgtctgg cgcagcaact gggcaatcac 420
aaacagctga tcgaatccgt catctcggtg gcacccgtag cagttgcact cctggatgtg 480
gatggcaagg tggtgttgga caatccggcc tatcggcgga tcgtttccga catgcgggtt 540
gccgaaccgg cgcacgcggt catggacgag ctgcgccgca gcctggggga cgatttcggc 600
cgtgcgcttg cacatcggcg ggcgttgctc aatcacgaac tgtgcttcga tctgggtgtg 660
tcggatccgc gctggtattc gtgttcggtg acctggttcg aggagcgcca caccagcccg 720
gatgcgttct atggtgacgg ctgttccgat tacatgctgc tcgtcatgca cgatatcagt 780
gcatcgaagc gccaacagga ggcgctgcgc atcgccgcga tgcgtgcaat gctgtccgag 840
ggcgagttca accagagtct cagggaggcc ttgtccgggg caatctttca gttgcagggg 900
ccggtcaacc tgattgccgc agccgcaagc ctgcagcggc ggcgggccgg tggcgctgca 960
gggggcgacg cattgagcag ggccctggct gatgcccagc gtgctggaga gcaggcgatc 1020
gcaaccttgc agtcggcgat gccggccgaa ccggaggcgc cgtcgggcct ggtcagcctg 1080
aacgaagtgc tgcgcgacgt gctgatgctg gaaaccgagt cctttctctc cgctggcgtg 1140
acggtcgact ggcatccggc gcatgtcctg ccttcggtac agggcaatcc cacggcgctg 1200
cgcaacctgt tccgccagtt ggtggccaat gcggtggagg ccatgaatgt acggggctgg 1260
aaggagcgca gcctcagtgt gtcgaccgaa ctcatcgggc aggatgtgtg cgtggtggtc 1320
agcgacagtg gtcccggcat tcccgaggca ctgagactga aggttttcga gcccttcttc 1380
tcgaccaagc agacgcgatc ggcagggcgg ggcgtcggcc tgtcgctggc gcaggagatc 1440
gtcagtcgtc accgcggcat gctcaagatt gaccccgagc acacgaacgg gtgctgcatc 1500
cgcgtcagct tcccgtcgat gcgccagaac gccgagggcg cgtcaggggg ttctcgatga 1560
 


<210> SEQ ID NO:2
<211> 20
<212> DNA 
<213>Artificalsequence
<220>
<223>NifL-Spacer: Sequence for identification by Cas9
<400>2

acaaggcctc cctgagactc 20



<210> SEQ ID NO:3
<211> 1551
<212> DNA 
<213> Wild type 
<220>
<223>NiFA:WT Gene encoding Glutaminase protein
<400> 3

atgaatgcaa acgagccaat caacgagctc gcgcagcgtg cagcctggct cgagtcctcg 60
cttgaggccc tgtatcgcgt cagtcgggtg ctgtcacgtt cgctcgagct gcgggaaacg 120
ctggcggagt tgctgcgcgt gcttgatgag gagtgtggat tcaaccgtgc gctggttacc 180
ctgaatgagc cgggaggtga ctcgatggcg atttcggctt tgcatggggt ggacgctccg 240
attaatccgg acattcgttg gcgggcgggt gagggcgtca tcggcaccgt gcagcaacgt 300
tcgcggccgc tcgtgctgca gcgcctggcc gacagtccga attacctgcc gcagcgaggc 360
gttttcgatg ccgaggcgcc gttcatcggc gtgccgatcc gtgccgcgca tgaaaccgtc 420
ggcgtgcttg cgctgcagcc accggtcgcc ttgtgcgagc gcctcgacga cgatgctcac 480
tttgccgaga tggtggcgaa cctcatcggt caggttgtcc gcttgtcgag ccaggtgaag 540
caggagcgtc gggacatctc cgaggagcgc gataccctga ggcgcgcggt acgaagtcat 600
ttcggcttcg acagcatcat cggtcacacg gcggcgatgc gtaaggtgtt cgaccaggtg 660
cggcaggtgg cgaagtggaa caccacggtg cttgtccgtg gcgagacggg gacgggcaag 720
gaactcatcg cgcaggccat tcattacaac tctccgcgcg cggccggccc cttcgtcaaa 780
ctcaactgtg ccgcgctgcc ggagaacctg ctggagtcgg agctcttcgg tcacgagaaa 840
ggggctttca ccggcgcgct gacgcagcgc aagggccgct tcgaagccgc tgatgcgggg 900
acgctgttcc tggacgagat tggcgaaatt tccgcgtcct ttcaggccaa gctgctgcgc 960
gtcttgcagg agggcgagct cgagcgcgtc ggcggcgccc gaaccttgcg cgtcgatgtt 1020
cgcgtgattg ccgccaccaa tcgcgatctg gaactcgagg tggaggccgg gaagttccgc 1080
gaagacctgt tctaccggct caatgtcatg cccatcatgt tgccgccatt gcgcgaacgg 1140
gtggaggaca ttcccgaaat cgcgaccttc ctggtcgaca aggtggccaa gatgcaggga 1200
cggcccctgt ccatcactga aagtgcattg cgtatcctgc ttcaccacgc ctggccggga 1260
aacgtgcgtg aactcgagaa ctgtatcgag cgcgccgcgg tcatgagcga ggatggggtc 1320
atcgatcgcg acctggtgct gatttcgggc gtggatgaac gtgtcacacc gatgcgcggg 1380
ggcggagcgg tcgatctcga cgatcctgat ctggatgagc gcgaacgtgt gatcgcggcg 1440
ctggagcagg ccgggtgggt gcaggccaag gcggcgcggc tgctgaacat gacaccgcgc 1500
cagatcgcct accgtatcca gacgctcaac atcaaggtgc ggcaaatctg a 	  1551



<210> SEQ ID NO:4
<211> 909
<212> DNA 
<213> Wild type 
<220>
<223>RnfA-WT:Encodes a system for electron transport to nitrogenase
<400> 4
tcagtgattg gtcaggccgg aaaagcccat gaaggccagt gacagcagtc cggcggtgat 60
gaatccgatc ggcgcgccgg caaaggcggc cggcacccgt gcgagtgcga ggcgttcgcg 120
caggcctgcg aagagcacca gtaccatggt gaagccggcc gccgagccca gaccgtaaat 180
cacgctgcgc gcgaagccgg cgccttcctg gacattgagc aacgcgacgc cgagcaccgc 240
gcagttggtg gtgatcagtg gcagatagat acccagcacc ttgtacagcg tgggcgcatt 300
cttctttacc gccatttcga cgaactgcac ggtggccgca atgacgagga tgaatcccag 360
aatgcgcaga tagccgagat cgaacggcgc caggagcgca tgttccagca cccaggtgac 420
cgcgctcgcc agcgtgagca cgaacgtcgt ggccatcccc atgcccagcg cactgtcgat 480
cttgcgcgac acccccatga aagggcacag gccaaggaac ttgaccagga cgacgttgtt 540
caccagcgcc gtaccgagca gcagcatcaa ccattcactc attgccgacg atctccgtca 600
atttcgatct tcacggccct tgttgcatga ggcgtgccat tcggggtgcg ttttcaaaag 660
gctgatttaa aagtgaattt tgtgcagtgc agtgtccact gcatgtgtcg attccgccac 720
gcgtcagtga tttgtcgtga aggctacaca tgaacggcgc gcgagatgga cgcgtacccg 780
ctgtcgcccg gcgcggtatc cctgacgaca gcgggtgttc gggcgaatgc accgcttgag 840
tgcgcaaaat tgtcgggttt ggcacatagc ttgctaaggg agtcgcaacc cttctctgtc 900
gagacgtca							  909



<210> SEQ ID NO:5
<211> 4016
<212> DNA 
<213> Wild type 
<220>
<223>Complete sequence of NIF L; NIF A and RnfA 
<400> 5
tcagtgattg gtcaggccgg aaaagcccat gaaggccagt gacagcagtc cggcggtgat 60
gaatccgatc ggcgcgccgg caaaggcggc cggcacccgt gcgagtgcga ggcgttcgcg 120
caggcctgcg aagagcacca gtaccatggt gaagccggcc gccgagccca gaccgtaaat 180
cacgctgcgc gcgaagccgg cgccttcctg gacattgagc aacgcgacgc cgagcaccgc 240
gcagttggtg gtgatcagtg gcagatagat acccagcacc ttgtacagcg tgggcgcatt 300
cttctttacc gccatttcga cgaactgcac ggtggccgca atgacgagga tgaatcccag 360
aatgcgcaga tagccgagat cgaacggcgc caggagcgca tgttccagca cccaggtgac 420
cgcgctcgcc agcgtgagca cgaacgtcgt ggccatcccc atgcccagcg cactgtcgat 480
cttgcgcgac acccccatga aagggcacag gccaaggaac ttgaccagga cgacgttgtt 540
caccagcgcc gtaccgagca gcagcatcaa ccattcactc attgccgacg atctccgtca 600
atttcgatct tcacggccct tgttgcatga ggcgtgccat tcggggtgcg ttttcaaaag 660
gctgatttaa aagtgaattt tgtgcagtgc agtgtccact gcatgtgtcg attccgccac 720
gcgtcagtga tttgtcgtga aggctacaca tgaacggcgc gcgagatgga cgcgtacccg 780
ctgtcgcccg gcgcggtatc cctgacgaca gcgggtgttc gggcgaatgc accgcttgag 840
tgcgcaaaat tgtcgggttt ggcacatagc ttgctaaggg agtcgcaacc cttctctgtc 900
gagacgtcaa tgtcagcgcc cgattccatg caagcagtgt ttgccgatgc agtgttccac 960
gatgcggttg agcaatcggc gatcgcgatc tcgattaccg acggcaatgc tcacatcctc 1020
tatgtaaacc ccgcgttctg tgcaattaca ggctatgcgc cgggcgaagt cgtcggcaag 1080
cggcaatcgc tgttgtctta caagtcgacg cccagaagcg tgtatcagaa gatgtggcaa 1140
gcgctcaggg cgggacagtc ctggaccggg cggctgttga accggcgtcg ggatggttcg 1200
ccttatgtgg ccgaactcac cgtgaccccc ttgcgttcgt cggtgagtcg ggaggacgag 1260
gtgcgctacc tgggcatgca ctgggatgcc accgaggagc atcgtctggc gcagcaactg 1320
ggcaatcaca aacagctgat cgaatccgtc atctcggtgg cacccgtagc agttgcactc 1380
ctggatgtgg atggcaaggt ggtgttggac aatccggcct atcggcggat cgtttccgac 1440
atgcgggttg ccgaaccggc gcacgcggtc atggacgagc tgcgccgcag cctgggggac 1500
gatttcggcc gtgcgcttgc acatcggcgg gcgttgctca atcacgaact gtgcttcgat 1560
ctgggtgtgt cggatccgcg ctggtattcg tgttcggtga cctggttcga ggagcgccac 1620
accagcccgg atgcgttcta tggtgacggc tgttccgatt acatgctgct cgtcatgcac 1680
gatatcagtg catcgaagcg ccaacaggag gcgctgcgca tcgccgcgat gcgtgcaatg 1740
ctgtccgagg gcgagttcaa ccagagtctc agggaggcct tgtccggggc aatctttcag 1800
ttgcaggggc cggtcaacct gattgccgca gccgcaagcc tgcagcggcg gcgggccggt 1860
ggcgctgcag ggggcgacgc attgagcagg gccctggctg atgcccagcg tgctggagag 1920
caggcgatcg caaccttgca gtcggcgatg ccggccgaac cggaggcgcc gtcgggcctg 1980
gtcagcctga acgaagtgct gcgcgacgtg ctgatgctgg aaaccgagtc ctttctctcc 2040
gctggcgtga cggtcgactg gcatccggcg catgtcctgc cttcggtaca gggcaatccc 2100
acggcgctgc gcaacctgtt ccgccagttg gtggccaatg cggtggaggc catgaatgta 2160
cggggctgga aggagcgcag cctcagtgtg tcgaccgaac tcatcgggca ggatgtgtgc 2220
gtggtggtca gcgacagtgg tcccggcatt cccgaggcac tgagactgaa ggttttcgag 2280
cccttcttct cgaccaagca gacgcgatcg gcagggcggg gcgtcggcct gtcgctggcg 2340
caggagatcg tcagtcgtca ccgcggcatg ctcaagattg accccgagca cacgaacggg 2400
tgctgcatcc gcgtcagctt cccgtcgatg cgccagaacg ccgagggcgc gtcagggggt 2460
tctcgatgaa tgcaaacgag ccaatcaacg agctcgcgca gcgtgcagcc tggctcgagt 2520
cctcgcttga ggccctgtat cgcgtcagtc gggtgctgtc acgttcgctc gagctgcggg 2580
aaacgctggc ggagttgctg cgcgtgcttg atgaggagtg tggattcaac cgtgcgctgg 2640
ttaccctgaa tgagccggga ggtgactcga tggcgatttc ggctttgcat ggggtggacg 2700
ctccgattaa tccggacatt cgttggcggg cgggtgaggg cgtcatcggc accgtgcagc 2760
aacgttcgcg gccgctcgtg ctgcagcgcc tggccgacag tccgaattac ctgccgcagc 2820
gaggcgtttt cgatgccgag gcgccgttca tcggcgtgcc gatccgtgcc gcgcatgaaa 2880
ccgtcggcgt gcttgcgctg cagccaccgg tcgccttgtg cgagcgcctc gacgacgatg 2940
ctcactttgc cgagatggtg gcgaacctca tcggtcaggt tgtccgcttg tcgagccagg 3000
tgaagcagga gcgtcgggac atctccgagg agcgcgatac cctgaggcgc gcggtacgaa 3060
gtcatttcgg cttcgacagc atcatcggtc acacggcggc gatgcgtaag gtgttcgacc 3120
aggtgcggca ggtggcgaag tggaacacca cggtgcttgt ccgtggcgag acggggacgg 3180
gcaaggaact catcgcgcag gccattcatt acaactctcc gcgcgcggcc ggccccttcg 3240
tcaaactcaa ctgtgccgcg ctgccggaga acctgctgga gtcggagctc ttcggtcacg 3300
agaaaggggc tttcaccggc gcgctgacgc agcgcaaggg ccgcttcgaa gccgctgatg 3360
cggggacgct gttcctggac gagattggcg aaatttccgc gtcctttcag gccaagctgc 3420
tgcgcgtctt gcaggagggc gagctcgagc gcgtcggcgg cgcccgaacc ttgcgcgtcg 3480
atgttcgcgt gattgccgcc accaatcgcg atctggaact cgaggtggag gccgggaagt 3540
tccgcgaaga cctgttctac cggctcaatg tcatgcccat catgttgccg ccattgcgcg 3600
aacgggtgga ggacattccc gaaatcgcga ccttcctggt cgacaaggtg gccaagatgc 3660
agggacggcc cctgtccatc actgaaagtg cattgcgtat cctgcttcac cacgcctggc 3720
cgggaaacgt gcgtgaactc gagaactgta tcgagcgcgc cgcggtcatg agcgaggatg 3780
gggtcatcga tcgcgacctg gtgctgattt cgggcgtgga tgaacgtgtc acaccgatgc 3840
gcgggggcgg agcggtcgat ctcgacgatc ctgatctgga tgagcgcgaa cgtgtgatcg 3900
cggcgctgga gcaggccggg tgggtgcagg ccaaggcggc gcggctgctg aacatgacac 3960
cgcgccagat cgcctaccgt atccagacgc tcaacatcaa ggtgcggcaa atctga 4016



<210> SEQ ID NO:6
<211> 12924
<212> DNA 
<213>Artificial sequence
<220>
<223>Complete sequence of NIF L; NIF A and RnfA_ pCasAz
<400> 6

acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg 60
ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa 120
aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta 180
taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat 240
agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac 300
tgtttctcca tacccgtttt tttgggaatt cgagctctaa ggaggttata aaaatggaca 360
agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg 420
acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca 480
tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc 540
ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc 600
aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag 660
agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg 720
tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg 780
tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca 840
agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca 900
agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg 960
ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg 1020
aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg 1080
ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca 1140
aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg 1200
gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga 1260
gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca 1320
agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc 1380
tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca 1440
ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga 1500
tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc 1560
ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc 1620
tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga 1680
tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg 1740
cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg 1800
acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc 1860
ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg 1920
agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg 1980
agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc 2040
agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg 2100
tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg 2160
acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc 2220
tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt 2280
tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga 2340
gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc 2400
tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga 2460
cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc 2520
acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg 2580
tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa 2640
tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc 2700
ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa 2760
acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt 2820
acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc 2880
ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga 2940
accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact 3000
ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg 3060
ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg 3120
aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt 3180
acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg 3240
tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc 3300
acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta 3360
agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg 3420
ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca 3480
tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga 3540
tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg 3600
tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag 3660
gcggcttcag caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa 3720
agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg 3780
tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc 3840
tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg 3900
aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc 3960
tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg 4020
gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg 4080
agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca 4140
agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg 4200
ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca 4260
gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg 4320
ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc 4380
tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt 4440
ctcagctggg aggcgactga ttctcatgtt tgacagctta tcatcgataa gctttaatgc 4500
ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtaatga aatctaacaa 4560
tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat 4620
gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta 4680
tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc 4740
actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat 4800
cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt 4860
ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga 4920
tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt 4980
ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc 5040
ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca 5100
taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg 5160
ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 5220
aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc 5280
gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt 5340
cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc 5400
ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc 5460
cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc 5520
caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag 5580
cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac 5640
atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt 5700
gcgtcgcggt gcatggagcc gggccacctc gacctgatta tgacaacttg acggctacat 5760
cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt 5820
taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga 5880
taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc 5940
agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca 6000
agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga 6060
tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg 6120
cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc 6180
gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt 6240
gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt 6300
catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg 6360
gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc 6420
aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa 6480
tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa 6540
tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat 6600
tttgcgcttc agccattttt ataacctcct tagagctcga attcccaaaa aaacgggtat 6660
ggagaaacag tagagagttg cgataaaaag cgtcaggtag gatccgctaa tcttatggat 6720
aaaaatgcta tggcatagca aagtgtgacg ccgtgcaaat aatcaatgtg gacttttctg 6780
ccgtgattat agacactttt gttacgcgtt tttgtcatgg ctttggtccc gctttgttac 6840
agaatgcttt taataagcgg ggttaccggt ttggttagcg agaagagcca gtaaaagacg 6900
cagtgacggc aatgtctgat gcaatatgga caattggttt cttctctgaa tggcgggagt 6960
atgaaaagta cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat 7020
cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc 7080
gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa 7140
aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg 7200
ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca 7260
actctctact gtttctccat acccgttttt ttgggaattc gagctctaag gaggttataa 7320
aaatggatat taatactgaa actgagatca agcaaaagca ttcactaacc ccctttcctg 7380
ttttcctaat cagcccggca tttcgcgggc gatattttca cagctatttc aggagttcag 7440
ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg cgtcactacc 7500
agcagctcgc ccgtgaagag aaagaggcag aactggcaga cgacatggaa aaaggcctgc 7560
cccagcacct gtttgaatcg ctatgcatcg atcatttgca acgccacggg gccagcaaaa 7620
aatccattac ccgtgcgttt gatgacgatg ttgagtttca ggagcgcatg gcagaacaca 7680
tccggtacat ggttgaaacc attgctcacc accaggttga tattgattca gaggtataaa 7740
gaggtataaa acgatgagta ctgcactcgc aacgctggct gggaagctgg ctgaacgtgt 7800
cggcatggat tctgtcgacc cacaggaact gatcaccact cttcgccaga cggcatttaa 7860
aggtgatgcc agcgatgcgc agttcatcgc attactgatc gttgccaacc agtacggcct 7920
taatccgtgg acgaaagaaa tttacgcctt tcctgataag cagaatggca tcgttccggt 7980
ggtgggcgtt gatggctggt cccgcatcat caatgaaaac cagcagtttg atggcatgga 8040
ctttgagcag gacaatgaat cctgtacatg ccggatttac cgcaaggacc gtaatcatcc 8100
gatctgcgtt accgaatgga tggatgaatg ccgccgcgaa ccattcaaaa ctcgcgaagg 8160
cagagaaatc acggggccgt ggcagtcgca tcccaaacgg atgttacgtc ataaagccat 8220
gattcagtgt gcccgtctgg ccttcggatt tgctggtatc tatgacaagg atgaagccga 8280
gcgcattgtc gaaaatactg catacactgc agaacgtcag ccggaacgcg acatcactcc 8340
ggttaacgat gaaaccatgc aggagattaa cactctgctg atcgccctgg ataaaacatg 8400
ggatgacgac ttattgccgc tctgttccca gatatttcgc cgcgacattc gtgcatcgtc 8460
agaactgaca caggccgaag cagtaaaagc tcttggattc ctgaaacaga aagccgcaga 8520
gcagaaggtg gcagcatgaa gaaggtggca gcatgacacc ggacattatc ctgcagcgta 8580
ccgggatcga tgtgagagct gtcgaacagg gggatgatgc gtggcacaaa ttacggctcg 8640
gcgtcatcac cgcttcagaa gttcacaacg tgatagcaaa accccgctcc ggaaagaagt 8700
ggcctgacat gaaaatgtcc tacttccaca ccctgcttgc tgaggtttgc accggtgtgg 8760
ctccggaagt taacgctaaa gcactggcct ggggaaaaca gtacgagaac gacgccagaa 8820
ccctgtttga attcacttcc ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg 8880
aaagtatgcg taccgcctgc tctcccgatg gtttatgcag tgacggcaac ggccttgaac 8940
tgaaatgccc gtttacctcc cgggatttca tgaagttccg gctcggtggt ttcgaggcca 9000
taaagtcagc ttacatggcc caggtgcagt acagcatgtg ggtgacgcga aaaaatgcct 9060
ggtactttgc caactatgac ccgcgtatga agcgtgaagg cctgcattat gtcgtgattg 9120
agcgggatga aaagtacatg gcgagttttg acgagatcgt gccggagttc atcgaaaaaa 9180
tggacgaggc actggctgaa attggttttg tatttgggga gcaatggcga tgataaatcg 9240
cgcgggtttg ttactgataa agcaggcaag acctaaaatg tgtaaagggc aaagtgtata 9300
ctttggcgtc accccttaca tattttaggt ctttttttat tgtgcgtaac taacttgcca 9360
tcttcaaaca ggagggctgg aagaagcaga ccgctaacac agtacataaa aaaggagaca 9420
tgaacgatga acatcaaaaa gtttgcaaaa caagcaacag tattaacctt tactaccgca 9480
ctgctggcag gaggcgcaac tcaagcgttt gcgaaagaaa cgaaccaaaa gccatataag 9540
gaaacatacg gcatttccca tattacacgc catgatatgc tgcaaatccc tgaacagcaa 9600
aaaaatgaaa aatatcaagt tcctgagttc gattcgtcca caattaaaaa tatctcttct 9660
gcaaaaggcc tggacgtttg ggacagctgg ccattacaaa acgctgacgg cactgtcgca 9720
aactatcacg gctaccacat cgtctttgca ttagccggag atcctaaaaa tgcggatgac 9780
acatcgattt acatgttcta tcaaaaagtc ggcgaaactt ctattgacag ctggaaaaac 9840
gctggccgcg tctttaaaga cagcgacaaa ttcgatgcaa atgattctat cctaaaagac 9900
caaacacaag aatggtcagg ttcagccaca tttacatctg acggaaaaat ccgtttattc 9960
tacactgatt tctccggtaa acattacggc aaacaaacac tgacaactgc acaagttaac 10020
gtatcagcat cagacagctc tttgaacatc aacggtgtag aggattataa atcaatcttt 10080
gacggtgacg gaaaaacgta tcaaaatgta cagcagttca tcgatgaagg caactacagc 10140
tcaggcgaca accatacgct gagagatcct cactacgtag aagataaagg ccacaaatac 10200
ttagtatttg aagcaaacac tggaactgaa gatggctacc aaggcgaaga atctttattt 10260
aacaaagcat actatggcaa aagcacatca ttcttccgtc aagaaagtca aaaacttctg 10320
caaagcgata aaaaacgcac ggctgagtta gcaaacggcg ctctcggtat gattgagcta 10380
aacgatgatt acacactgaa aaaagtgatg aaaccgctga ttgcatctaa cacagtaaca 10440
gatgaaattg aacgcgcgaa cgtctttaaa atgaacggca aatggtatct gttcactgac 10500
tcccgcggat caaaaatgac gattgacggc attacgtcta acgatattta catgcttggt 10560
tatgtttcta attctttaac tggcccatac aagccgctga acaaaactgg ccttgtgtta 10620
aaaatggatc ttgatcctaa cgatgtaacc tttacttact cacacttcgc tgtacctcaa 10680
gcgaaaggaa acaatgtcgt gattacaagc tatatgacaa acagaggatt ctacgcagac 10740
aaacaatcaa cgtttgcgcc tagcttcctg ctgaacatca aaggcaagaa aacatctgtt 10800
gtcaaagaca gcatccttga acaaggacaa ttaacagtta acaaataatc atggctctgc 10860
cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct cttcgatctt 10920
cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag 10980
ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg 11040
gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg ccagcaggta 11100
ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc gttcgtctgg 11160
aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat 11220
ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc 11280
gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg 11340
gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg 11400
aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc 11460
tataatgacc ccgaagcagg gttatgcagc ggaaaagatc cgtccttttt tagccgctaa 11520
aacggccggg gggtgcgcgt gattgccaag cacgtcccca tgcgctccat caagaagagc 11580
gacttcgcgg agctggtatt cgtgcagggc aagattcgga ataccaagta cgagaaggac 11640
ggccagacgg tctacgggac cgacttcatt gccgataagg tggattatct ggacaccaag 11700
gcaccaggcg ggtcaaatca ggaataaggg cacattgccc cggcgtgagt cggggcaatc 11760
ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca agaactgatc 11820
gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt catgcgtgcg 11880
ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc caagatcgag 11940
cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc cgtggagcgt 12000
tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat cgacacgcga 12060
ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa acaggtcagc 12120
gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga aatgcagctt 12180
tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa cgacacggcc 12240
cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct gcaaaacaag 12300
gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga gctgcgggcc 12360
gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac ccctatcggc 12420
gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc gatcaatggc 12480
cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc gatgggcttc 12540
acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt ccgcgtcctg 12600
gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat cgtcgtgctg 12660
tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct gtcgccgacg 12720
gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct caagctggaa 12780
accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg cgagcaggtc 12840
ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg ggtcaatgat 12900
gacctggtgc attgcaaacg ctac 					  12924



<210> SEQ ID NO:7
<211> 5678
<212> DNA 
<213> Artificial 
<220>
<223> complete sequence of pAzCRISPR vector
<400> 7

ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 60
agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 120
cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 180
ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 240
gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 300
cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 360
cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 420
ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 480
catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 540
tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 600
ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 660
catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 720
cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 780
cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 840
acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 900
ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 960
tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 1020
attaacctat aaaaataggc gtatcacgag gccctttcgt ctaaatcgcg cgggtttgtt 1080
actgataaag caggcaagac ctaaaatgtg taaagggcaa agtgtatact ttggcgtcac 1140
cccttacata ttttaggtct ttttttattg tgcgtaacta acttgccatc ttcaaacagg 1200
agggctggaa gaagcagacc gctaacacag tacataaaaa aggagacatg aacgatgaac 1260
atcaaaaagt ttgcaaaaca agcaacagta ttaaccttta ctaccgcact gctggcagga 1320
ggcgcaactc aagcgtttgc gaaagaaacg aaccaaaagc catataagga aacatacggc 1380
atttcccata ttacacgcca tgatatgctg caaatccctg aacagcaaaa aaatgaaaaa 1440
tatcaagttc ctgagttcga ttcgtccaca attaaaaata tctcttctgc aaaaggcctg 1500
gacgtttggg acagctggcc attacaaaac gctgacggca ctgtcgcaaa ctatcacggc 1560
taccacatcg tctttgcatt agccggagat cctaaaaatg cggatgacac atcgatttac 1620
atgttctatc aaaaagtcgg cgaaacttct attgacagct ggaaaaacgc tggccgcgtc 1680
tttaaagaca gcgacaaatt cgatgcaaat gattctatcc taaaagacca aacacaagaa 1740
tggtcaggtt cagccacatt tacatctgac ggaaaaatcc gtttattcta cactgatttc 1800
tccggtaaac attacggcaa acaaacactg acaactgcac aagttaacgt atcagcatca 1860
gacagctctt tgaacatcaa cggtgtagag gattataaat caatctttga cggtgacgga 1920
aaaacgtatc aaaatgtaca gcagttcatc gatgaaggca actacagctc aggcgacaac 1980
catacgctga gagatcctca ctacgtagaa gataaaggcc acaaatactt agtatttgaa 2040
gcaaacactg gaactgaaga tggctaccaa ggcgaagaat ctttatttaa caaagcatac 2100
tatggcaaaa gcacatcatt cttccgtcaa gaaagtcaaa aacttctgca aagcgataaa 2160
aaacgcacgg ctgagttagc aaacggcgct ctcggtatga ttgagctaaa cgatgattac 2220
acactgaaaa aagtgatgaa accgctgatt gcatctaaca cagtaacaga tgaaattgaa 2280
cgcgcgaacg tctttaaaat gaacggcaaa tggtatctgt tcactgactc ccgcggatca 2340
aaaatgacga ttgacggcat tacgtctaac gatatttaca tgcttggtta tgtttctaat 2400
tctttaactg gcccatacaa gccgctgaac aaaactggcc ttgtgttaaa aatggatctt 2460
gatcctaacg atgtaacctt tacttactca cacttcgctg tacctcaagc gaaaggaaac 2520
aatgtcgtga ttacaagcta tatgacaaac agaggattct acgcagacaa acaatcaacg 2580
tttgcgccta gcttcctgct gaacatcaaa ggcaagaaaa catctgttgt caaagacagc 2640
atccttgaac aaggacaatt aacagttaac aaataatcat ggctctgccc tcgggcggac 2700
cacgcccatc atgaccttgc caagctcgtc ctgcttctct tcgatcttcg ccagcagggc 2760
gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag 2820
caggccgccc aggcggccca ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata 2880
gtccacgacg cccgtgattt tgtagccctg gccgacggcc agcaggtagg ccgacaggct 2940
catgccggcc gccgccgcct tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac 3000
cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc 3060
atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc 3120
caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc 3180
tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca 3240
aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc 3300
gaagcagggt tatgcagcgg aaaagatccg tcttgacaat taatcatccg gctcgtataa 3360
tgtgtggaga ccattggtct ctgttttaga gctagaaata gcaagttaaa ataaggctag 3420
tccgttatca acttgaaaaa gtggcaccga gtcggtgccc tctcaggcgc cgctggtgcc 3480
gctggttgga cgccaagggt gaatccgcct cgataccctg attactcgct tcctgcgccc 3540
tctcaggcgg cgatagggga ctggtaaaac ggggattgcc cagacgcctc ccccgcccct 3600
tcaggggcac aaatgcggcc ccaacggggc cacgtagtgg tgcgtttttt gcgtttccac 3660
ccttttcttc cttttccctt ttaaaccttt taggacgtct acaggccacg taatccgtgg 3720
cctgtagagt ttaaaaaggg acggatttgt tgccattaag ggacggattt gttgttaaga 3780
agggacggat ttgttgttgt aaagggacgg atttgttgta ttgtgggacg cagatacagt 3840
gtccccttat acacaaggaa tgtcgaacgt ggcctcaccc ccaatggttt acaaaagcaa 3900
tgccctggtc gaggccgcgt atcgcctcag tgttcaggaa cagcggatcg ttctggcctg 3960
tattagccag gtgaagagga gcgagcctgt caccgatgaa gtgatgtatt cagtgacggc 4020
ggaggacata gcgacgatgg cgggtgtccc tatcgaatct tcctacaacc agctcaaaga 4080
agcggccctg cgcctgaaac ggcgggaagt ccggttaacc caagagccca atggcaaggg 4140
gaaaagaccg agtgtgatga ttaccggctg ggtgcaaaca atcatctacc gggagggtga 4200
gggccgtgta gaactcaggt tcaccaaaga catgctgccg tacctgacgg aactcaccaa 4260
acagttcacc aaatacgcct tggctgacgt ggccaagatg gacagcaccc acgcgatcag 4320
gctttacgag ctgctcatgc aatgggacag catcggccag cgcgaaatag aaattgacca 4380
gctgcgaaag tggtttcaac tggaaggccg gtatccctcg atcaaggact tcaagttgcg 4440
agtgcttgat ccagccgtga cgcagatcaa cgagcacagc ccgctacagg tggagtgggc 4500
gcagcgaaag accgggcgca aggtcacaca tctgttgttc agttttggac cgaagaagcc 4560
cgccaaggcg gtgggtaagg ccccagcgaa gcgcaaggcc gggaagattt cagatgctga 4620
gatcgcgaaa caggctcgcc ctggtgagac atgggaagcg gcccgcgctc gactaaccca 4680
gatgccgctg gatctggcct agtgagatcc tttttttctg cgcgtaatct gctgcttgca 4740
aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 4800
ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 4860
gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 4920
aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 4980
aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 5040
gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 5100
aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 5160
aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 5220
cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 5280
cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 5340
tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 5400
tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 5460
ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 5520
ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt 5580
taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 5640
gcaaaatccc ttataaatca aaagaataga ccgagata 			  5678



<210> SEQ ID NO: 8
<211>30
<212> DNA 
<213> Artificial 
<220>
<223> NifA Forward primer
<400> 8

gcaagcttat gaatgcaaac gagccaatca 30


<210> SEQ ID NO: 9
<211>30
<212> DNA 
<213> Artificial 
<220>
<223> NifA Rverse Primer
<400>9

cgtctagatc agatttgccg caccttgatg 30



<210> SEQ ID NO: 10
<211>30
<212> DNA 
<213> Artificial 
<220>
<223> RnfA Forward Primer
<400>10

attaagcttt gacgtctcga cagagaaggg 30



<210> SEQ ID NO: 11
<211>30
<212> DNA 
<213> Artificial 
<220>
<223> RnfA Reverse Primer
<400> 11

tatctcgagt cagtgattgg tcaggccgga 30



<210> SEQ ID NO: 12
<211>24
<212> DNA 
<213> Artificial 
<220>
<223> NifL Spacer F
<400> 12

gtggacaagg cctccctgag actc 24



<210> SEQ ID NO: 13
<211>24
<212> DNA 
<213> Artificial 
<220>
<223> NifL Spacer R
<400> 13

aaacgagtct cagggaggcc ttgt 24 



<210> SEQ ID NO: 14
<211>903
<212> DNA 
<213> Artificial 
<220>
<223>Uncultivable endophyte-1 isolated from sugarcane (Pseudomonas sp.)16s rRNA sequence
<400> 14

gaaacgaacg ctaataccgc atacgtccta cagacgaaag caggggactt tcgggccttg 60
cgctatcaca tgaccctaga gtcggattag ctagtatggt gaggtaatgg ctcaccaagg 120
cgacgatccg taactggtct gacaggatga acagtcacac tggaactgag acacggtcca 180
gactgctacg ggaggcagca gtggggaata ttggacaatg ggcgcaaagc cacatccagc 240
catgccgcgt gtgtgaagaa ggtgttcgga ttgaaaagca cttcaagttg ggaggaaggg 300
tgtgtagatt aatactctgc aatattgacg ttaccgacag aataagcacg ggctaactct 360
gtgccaggag ccgcggtaat acagagggtg caagcgttaa tcggaattac tgggcgtaat 420
acgcgcgtag gtggtttgtt aagttggatg tgaaatcccc gggctcaacc tgggaactgc 480
atccaaaact ggcaagctac actatggtag agggtggtgg aatttcctgt gtagcggtga 540
aatgcgtaga tataggaagt aacaccagtg gcgaaggcga ccacctggac tgatactgac 600
actgaggtgc gaaagcgtgg ggagcaatct ggattagata ccttggtagt ccacgccgta 660
aacgatgtca actagccgtt gggagccttg agctcttagt ggcgcagcta acgcattaag 720
ttgaccgcct cgggagtacg gccgcaaggt taatactcaa atgaatagac gggcgcccgc 780
acaagcggag gagcatgtgg tttaattcga agcaacgcga agaaccttac caggcctagt 840
catccaatga gctttccagt gatggattgg tgccatcggg aacattgaga caggtgctgc 900
atg								  903



<210> SEQ ID NO: 15
<211> 1330
<212> DNA 
<213> Artificial 
<220>
<223> Uncultivable endophyte-1 isolated from sugarcane (Pseudomonas sp.) 16s rRNA sequence
<400> 15

gacgaacgct ggcggcgtgc ctaatacatg caagtcgagc ggacagatgg gagcttgctc 60
cctgatgtta gcggcggacg ggtgagtaac acgtgggtaa cctgcctgta agactgggat 120
aactccgggg cgccggggct gggaccggat gcttgtttga accgcatggt tcagacataa 180
aaggtggctt cggctaccac ttacccatgg acccgcggcg cattagctag ttggtgaggt 240
aacggctcac caaggcgacg atgcgtagcc gacctgagag ggtcttcggc cacactggga 300
ctgagacacg gcccagtgac ctacgggagg cagcagtagg gaatgccctt caatggacga 360
aagtctgacg gagcaacgcc gcgtgagtga tgaaggtttt cggatcgtaa agctctgttg 420
ttagggaaga atgagtgccg ttcaaatagg gcggcacctt gacggtacct aaccagaaag 480
ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcacgcg ttgtccggaa 540
ttattgggcg taaagggctc gcaggcggtt tcttaagtct gatgtgaaag cccccggctc 600
aaccggggag ggtcattgga aactggggaa cttgagtgca gaagaggaga gtggaattcc 660
acgtgtagcg gtgaaatgcg tagagatgtg gaggaacacc agtggcgaag gcgactctct 720
ggtctgtaac tgacgctgag gagcgaaagc gtggggagcg aacacccaaa gataccctgg 780
tagtccacgc cgtaaacgat gagtgctaag tgttaggggt tatccgcccc ttagtgctgc 840
agctaacgca ttaagcactc cgcctgggga gtacggtcgc aagactgaaa ctcaaaggaa 900
ttgacggggg tatacacaag cgaaagagca tgtggtttaa ttcgaagcaa cgcgaagaac 960
cttaccaggt cttgacatcc tctgacaatc ctagagatag gacgtcccct tcggattcag 1020
agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg gggagtcccg 1080
caacgagcgc aacccttgat cttagttgcc agcacggagt tgggcactct aaggtgactg 1140
ccggtgacaa accggaggaa ggtggggatg acgtcaaatc atcatgcccc ttatgacctg 1200
ggctacacgg gtgctacaat gggcagaaca aagggcagcg aaaccgaaag gttaagccaa 1260
ggccacaaat ctgttctcag ttcggatcgc agtctgcaac tcgactgcgt gaagctggaa 1320
tcgctagtaa							  1330



<210> SEQ ID NO: 16
<211> 1470
<212> DNA 
<213> Artificial 
<220>
<223>Uncultivable endophyte-3 isolated from red gram (Sphingomonas sp.).16s rRNA sequence
<400> 16

agagttatca tggctcagaa tgaacgctgg cggcatgcct aacacatgca agtcgaacga 60
agcctcaggg tttagtggcg cacgggtgcg taacgcgtgg gaatctgccc cttggttcgg 120
aataacagtt ggaaacgact gctaataccg gatgatgacg taagtccaaa gatttatcgt 180
taagggatga gcccgcgtag gattaggtag ttggtgtggt aaaggcgcac caagccgacg 240
atccttagct ggtctgagag gatgatcagc cacactggga ctgagacacg gcccagactc 300
ctacgggagg cagcagtggg gaatattgga caatgggcga aagcctgatc cagcaatgcc 360
gcgtgagtgt ttaaggcctt agggttgtaa agctctttta cccgggatga taatgacagt 420
accgggagaa taagctccgg ctaactccgt gccagcagcc gcggtaatac ggagggagct 480
agcgttattc ggaattactg ggcgtaaagc gcacgtaggc ggctttgtaa gtaagaggtg 540
aaagcccaga gctcaactct ggaattgcct tttagactgc atcgcttgaa tcatggagag 600
gtcagtggaa ttccgagtgt agaggtgaaa ttcgtagata ttcggaagaa caccagtggc 660
gaaggcggct gactggacat gtattgacgc tgaggtgcga aagcgtgggg agcaaacagg 720
attagatacc ctggtagtcc acgccgtaaa cgatgataac tagctgtccg gggacttggt 780
ctttgggtgg cgcagctaac gcattaagtt atccgcctgg ggagtacggc cgcaaggtta 840
aaactcaaat gaattgacgg gggcctgcac aagcggtgga gcatgtggtt taattcgaag 900
caacgcgcag aaccttacca gcgtttgaca tggcaggacg acttctggag acagatttct 960
tcccttcggg gacctgcaca caggtgctgc atggctgtcg tcagctcgtg tcgtgagatg 1020
ttgggttaag tcccgcaact ttcgcaaccc tygaccttag ttgccatcat ttagttgggc 1080
actttaaggt aaccgccggt gataagccgg aggaaggtgg ggatgacgyt naagtcctca 1140
tggcccttac gcgctgggct acacacgtgc tacaatggcg gtgacagtgg gcagcaagca 1200
cgcgagtgtg agctaatctc caaaagccgt ctcagttcgg attgttctct gcaactcgag 1260
agcatcaagg cggaatcgct agtaatcgng gatcagcatg ccgcggngaa tacgttccca 1320
ccccttgtac acaccgnccg tcacaccatg ggagttggat tcacccgaag gcgttgcgct 1380
aactcgcaag agaggcangc gaccacggtg ggtttagcgt atggggtgaa gtcccaacaa 1440
ggtagccgta ggggaatttg cggctggaac 				  1470

