                         SEQUENCE LISTING

<110>  BIOCON BIOLOGICS INDIA LIMITED
 
<120>  MAMMALIAN EXPRESSION VECTORS

<130>  PCT00887

<150>  IN 201941022723
<151>  2019-06-07

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1624
<212>  DNA
<213>  artificial

<220>
<223>  CHO glutamine synthetase linked to a PGK promoter

<400>  1
gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg       60

gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc      120

gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca      180

ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac      240

gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt      300

ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg      360

gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct      420

ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt      480

cctcatctcc gggcctttct cgatggccac ctcagcaagt tcccacttga acaaaaacat      540

caagcaaatg tacttgtgcc tgccccaggg tgagaaagtc caagccatgt atatctgggt      600

tgatggtact ggagaaggac tgcgctgcaa aacccgcacc ctggactgtg agcccaagtg      660

tgtagaagag ttacctgagt ggaattttga tggctctagt acctttcagt ctgagggctc      720

caacagtgac atgtatctca gccctgttgc catgtttcgg gaccccttcc gcagagatcc      780

caacaagctg gtgttctgtg aagttttcaa gtacaaccgg aagcctgcag agaccaattt      840

aaggcactcg tgtaaacgga taatggacat ggtgagcaac cagcacccct ggtttggaat      900

ggaacaggag tatactctga tgggaacaga tgggcaccct tttggttggc cttccaatgg      960

ctttcctggg ccccaaggtc cgtattactg tggtgtgggc gcagacaaag cctatggcag     1020

ggatatcgtg gaggctcact accgcgcctg cttgtatgct ggggtcaaga ttacaggaac     1080

aaatgctgag gtcatgcctg cccagtggga attccaaata ggaccctgtg aaggaatccg     1140

catgggagat catctctggg tggcccgttt catcttgcat cgagtatgtg aagactttgg     1200

ggtaatagca acctttgacc ccaagcccat tcctgggaac tggaatggtg caggctgcca     1260

taccaacttt agcaccaagg ccatgcggga ggagaatggt ctgaagcaca tcgaggaggc     1320

catcgagaaa ctaagcaagc ggcaccggta ccacattcga gcctacgatc ccaagggggg     1380

cctggacaat gcccgtcgtc tgactgggtt ccacgaaacg tccaacatca acgacttttc     1440

tgctggtgtc gccaatcgca gtgccagcat ccgcattccc cggactgtcg gccaggagaa     1500

gaaaggttac tttgaagacc gccgcccctc tgccaattgt gacccctttg cagtgacaga     1560

agccatcgtc cgcacatgcc ttctcaatga gactggcgac gagcccttcc aatacaaaaa     1620

ctaa                                                                  1624


<210>  2
<211>  496
<212>  DNA
<213>  artificial

<220>
<223>  PGK promoter

<400>  2
gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg       60

gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc      120

gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca      180

ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac      240

gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt      300

ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg      360

gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct      420

ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt      480

cctcatctcc gggcct                                                      496


<210>  3
<211>  1122
<212>  DNA
<213>  artificial

<220>
<223>  GS ORF

<400>  3
atggccacct cagcaagttc ccacttgaac aaaaacatca agcaaatgta cttgtgcctg       60

ccccagggtg agaaagtcca agccatgtat atctgggttg atggtactgg agaaggactg      120

cgctgcaaaa cccgcaccct ggactgtgag cccaagtgtg tagaagagtt acctgagtgg      180

aattttgatg gctctagtac ctttcagtct gagggctcca acagtgacat gtatctcagc      240

cctgttgcca tgtttcggga ccccttccgc agagatccca acaagctggt gttctgtgaa      300

gttttcaagt acaaccggaa gcctgcagag accaatttaa ggcactcgtg taaacggata      360

atggacatgg tgagcaacca gcacccctgg tttggaatgg aacaggagta tactctgatg      420

ggaacagatg ggcacccttt tggttggcct tccaatggct ttcctgggcc ccaaggtccg      480

tattactgtg gtgtgggcgc agacaaagcc tatggcaggg atatcgtgga ggctcactac      540

cgcgcctgct tgtatgctgg ggtcaagatt acaggaacaa atgctgaggt catgcctgcc      600

cagtgggaat tccaaatagg accctgtgaa ggaatccgca tgggagatca tctctgggtg      660

gcccgtttca tcttgcatcg agtatgtgaa gactttgggg taatagcaac ctttgacccc      720

aagcccattc ctgggaactg gaatggtgca ggctgccata ccaactttag caccaaggcc      780

atgcgggagg agaatggtct gaagcacatc gaggaggcca tcgagaaact aagcaagcgg      840

caccggtacc acattcgagc ctacgatccc aaggggggcc tggacaatgc ccgtcgtctg      900

actgggttcc acgaaacgtc caacatcaac gacttttctg ctggtgtcgc caatcgcagt      960

gccagcatcc gcattccccg gactgtcggc caggagaaga aaggttactt tgaagaccgc     1020

cgcccctctg ccaattgtga cccctttgca gtgacagaag ccatcgtccg cacatgcctt     1080

ctcaatgaga ctggcgacga gcccttccaa tacaaaaact aa                        1122


<210>  4
<211>  204
<212>  DNA
<213>  artificial

<220>
<223>  CMV promoter

<400>  4
gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt       60

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      120

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      180

tgggaggtct atataagcag agct                                             204


<210>  5
<211>  373
<212>  PRT
<213>  artificial

<220>
<223>  CHO glutamine synthetase

<400>  5

Met Ala Thr Ser Ala Ser Ser His Leu Asn Lys Asn Ile Lys Gln Met 
1               5                   10                  15      


Tyr Leu Cys Leu Pro Gln Gly Glu Lys Val Gln Ala Met Tyr Ile Trp 
            20                  25                  30          


Val Asp Gly Thr Gly Glu Gly Leu Arg Cys Lys Thr Arg Thr Leu Asp 
        35                  40                  45              


Cys Glu Pro Lys Cys Val Glu Glu Leu Pro Glu Trp Asn Phe Asp Gly 
    50                  55                  60                  


Ser Ser Thr Phe Gln Ser Glu Gly Ser Asn Ser Asp Met Tyr Leu Ser 
65                  70                  75                  80  


Pro Val Ala Met Phe Arg Asp Pro Phe Arg Arg Asp Pro Asn Lys Leu 
                85                  90                  95      


Val Phe Cys Glu Val Phe Lys Tyr Asn Arg Lys Pro Ala Glu Thr Asn 
            100                 105                 110         


Leu Arg His Ser Cys Lys Arg Ile Met Asp Met Val Ser Asn Gln His 
        115                 120                 125             


Pro Trp Phe Gly Met Glu Gln Glu Tyr Thr Leu Met Gly Thr Asp Gly 
    130                 135                 140                 


His Pro Phe Gly Trp Pro Ser Asn Gly Phe Pro Gly Pro Gln Gly Pro 
145                 150                 155                 160 


Tyr Tyr Cys Gly Val Gly Ala Asp Lys Ala Tyr Gly Arg Asp Ile Val 
                165                 170                 175     


Glu Ala His Tyr Arg Ala Cys Leu Tyr Ala Gly Val Lys Ile Thr Gly 
            180                 185                 190         


Thr Asn Ala Glu Val Met Pro Ala Gln Trp Glu Phe Gln Ile Gly Pro 
        195                 200                 205             


Cys Glu Gly Ile Arg Met Gly Asp His Leu Trp Val Ala Arg Phe Ile 
    210                 215                 220                 


Leu His Arg Val Cys Glu Asp Phe Gly Val Ile Ala Thr Phe Asp Pro 
225                 230                 235                 240 


Lys Pro Ile Pro Gly Asn Trp Asn Gly Ala Gly Cys His Thr Asn Phe 
                245                 250                 255     


Ser Thr Lys Ala Met Arg Glu Glu Asn Gly Leu Lys His Ile Glu Glu 
            260                 265                 270         


Ala Ile Glu Lys Leu Ser Lys Arg His Arg Tyr His Ile Arg Ala Tyr 
        275                 280                 285             


Asp Pro Lys Gly Gly Leu Asp Asn Ala Arg Gly Leu Thr Gly Phe His 
    290                 295                 300                 


Glu Thr Ser Asn Ile Asn Asp Phe Ser Ala Gly Val Ala Asn Arg Ser 
305                 310                 315                 320 


Ala Ser Ile Arg Ile Pro Arg Thr Val Gly Gln Glu Lys Lys Gly Tyr 
                325                 330                 335     


Phe Glu Asp Arg Arg Pro Ser Ala Asn Cys Asp Pro Phe Ala Val Thr 
            340                 345                 350         


Glu Ala Ile Val Arg Thr Cys Leu Leu Asn Glu Thr Gly Asp Glu Pro 
        355                 360                 365             


Phe Gln Tyr Lys Asn 
    370             


<210>  6
<211>  286
<212>  PRT
<213>  artificial

<220>
<223>  AmpR

<400>  6

Met Ser Ile Gln His Phe Arg Val Ala Leu Ile Pro Phe Phe Ala Ala 
1               5                   10                  15      


Phe Cys Leu Pro Val Phe Ala His Pro Glu Thr Leu Val Lys Val Lys 
            20                  25                  30          


Asp Ala Glu Asp Gln Leu Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp 
        35                  40                  45              


Leu Asn Ser Gly Lys Ile Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe 
    50                  55                  60                  


Pro Met Met Ser Thr Phe Lys Val Leu Leu Cys Gly Ala Val Leu Ser 
65                  70                  75                  80  


Arg Ile Asp Ala Gly Gln Glu Gln Leu Gly Arg Arg Ile His Tyr Ser 
                85                  90                  95      


Gln Asn Asp Leu Val Glu Tyr Ser Pro Val Thr Glu Lys His Leu Thr 
            100                 105                 110         


Asp Gly Met Thr Val Arg Glu Leu Cys Ser Ala Ala Ile Thr Met Ser 
        115                 120                 125             


Asp Asn Thr Ala Ala Asn Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys 
    130                 135                 140                 


Glu Leu Thr Ala Phe Leu His Asn Met Gly Asp His Val Thr Arg Leu 
145                 150                 155                 160 


Asp Arg Trp Glu Pro Glu Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg 
                165                 170                 175     


Asp Thr Thr Met Pro Val Ala Met Ala Thr Thr Leu Arg Lys Leu Leu 
            180                 185                 190         


Thr Gly Glu Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp 
        195                 200                 205             


Met Glu Ala Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro 
    210                 215                 220                 


Ala Gly Trp Phe Ile Ala Asp Lys Ser Gly Ala Gly Glu Arg Gly Ser 
225                 230                 235                 240 


Arg Gly Ile Ile Ala Ala Leu Gly Pro Asp Gly Lys Pro Ser Arg Ile 
                245                 250                 255     


Val Val Ile Tyr Thr Thr Gly Ser Gln Ala Thr Met Asp Glu Arg Asn 
            260                 265                 270         


Arg Gln Ile Ala Glu Ile Gly Ala Ser Leu Ile Lys His Trp 
        275                 280                 285     


<210>  7
<211>  861
<212>  DNA
<213>  artificial

<220>
<223>  AmpR

<400>  7
atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct       60

gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca      120

cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc      180

gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc      240

cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg      300

gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta      360

tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc      420

ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt      480

gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg      540

cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct      600

tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc      660

tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttct      720

cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac      780

acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc      840

tcactgatta agcattggta a                                                861

<210>  8
<211>  861
<212>  DNA
<213>  Artificial

<220>
<223>  AmpR, complementary sequence

<400>  8
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat       60

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc      120

cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat cagcaataaa      180

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca      240

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa      300

cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt      360

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc      420

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact      480

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc      540

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg      600

ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct      660

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc      720

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag      780

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac      840

acggaaatgt tgaatactca t                                                861

<210>  9
<211>  105
<212>  DNA
<213>  artificial

<220>
<223>  AmpR promoter

<400>  9
cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga       60

caataaccct gataaatgct tcaataatat tgaaaaagga agagt                      105


<210>  10
<211>  105
<212>  DNA
<213>  artificial

<220>
<223>  AmpR promoter, complementary sequence

<400>  10
actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata       60

catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcg                      105


<210>  11
<211>  589
<212>  DNA
<213>  artificial

<220>
<223>  ori

<400>  11
ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc       60

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt      120

cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt      180

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc      240

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa      300

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac      360

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg      420

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga      480

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact      540

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa                  589


<210>  12
<211>  589
<212>  DNA
<213>  artificial

<220>
<223>  ori, complementary sequence

<400>  12
tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg       60

gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg      120

ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag      180

cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc      240

caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa      300

ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg      360

taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc      420

taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac      480

cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg      540

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaa                  589


<210>  13
<211>  3603
<212>  DNA
<213>  artificial

<220>
<223>  EASE

<400>  13
caattctaca gaaaattgtt gcacctgttc agagtctaat tattgttttg caggcacagt       60

atgacaagta agtatctgtt gaatacattg taattcctaa attctatgga acttaaattt      120

gttctcagga tacaaaatgt acttgaaatt ttccttattt accttgtaaa cataataaaa      180

gttggtatta aaccataaag tttataacac caagcaaata attcaaacta acatggctca      240

tacatgaagt catctggcta aagaatgttt cttgaagttt atttctagaa cagcaaatgc      300

caactatctt ttaaaaaaat aatttaagaa aatgagcaaa agagcagttt aattaagtaa      360

cacctaatta gctttaagga gaccaaggca aagcctgcca aaagattaac accattactt      420

atctgttcga aaactactga cgacttacat tctggatgta tttaaaaaaa aaaaaaaaaa      480

ctaaattgct ttatcctatc aagttgtaca agatacccac gagacagaca atatagtatg      540

tcaatcagtt aagacgttaa atgacaaaaa catgttaaat caaaaataat tcttctttcc      600

tgctattttt aaatgttata aaatgcagct aggagctttt actattactc tgtggtgcaa      660

agtctacatg gtaattggca gcagccaaaa cagaatggca taccggcact tgatattgct      720

gtgtaaagaa aatataagtt tccagaggta caaaaatact actttgcagg tgccaaccaa      780

ccaccaggac tttagctggc tgtaaaggca gcttatttct gcaatcaaac cgaaagcatt      840

ttcacacaga agatggagtc tgcagcccta tgtgatctga agcagggcta atctgcacag      900

agcaaacaat cagggagggc cagccctgct gggaaggtcg cattctccca cagcaacaag      960

gcagggcaac taagcagcac attgcagcaa gaggacaaca gacagcctgt ccccagaacc     1020

gcccagccgc atgcacaaca accaacagag gattcagagc aaggggatgc tcttacctct     1080

tttatgtaat tccctcaagg ggatattgtc tcctcccccg tcataaggca aatagggatc     1140

ggaagaagca tccatgatca gaattttttt aaaagaaaaa aaaaaagatg aagaaaagag     1200

aagcttgcta gattaaaaag atggtggcat tttttcctcc ccctgctata gcaggcaagg     1260

gagatgccat atttgatgag gttggaactg tatagttcga ctggctgaag caactgggtt     1320

cttccacagt ggatgatgtc atcaaaggga aattattgca gcctggttgc tagtggctac     1380

aatctagccc ggcttagagg cagcacagcc ttcttaatca ggtcctgttg tgtacgacga     1440

tgactaagcg ccaagtataa caggcgaaac tgctttgaga aatttaaagt gctcacaacg     1500

tagtttcagt agtaagacag cagaaacatt tatttttatt tggaaaaaat ataagacata     1560

aaaaggtaat tccaaatgct aacaatggca ctttagccaa acagaaaact ctaaaaaggc     1620

tatgttttta taaatgtact aatttatgca taaattcatg agtcataaca gtgaaaacta     1680

aagcacacca acaagtttct ccagagtctt ggctttaaga atttgtttaa ttttccttta     1740

atccttttat ggaaaattta aatgctgagt cactgagttg aatgagtggt gttttatctc     1800

atttaccaat cccctccagc ggcctcccgt tccctttttc aaacccagcc acaagacact     1860

ccagttgggg gaaaaagtga aaacttaccc gtatactaat aactcctaca tgtgcaaatt     1920

atgcaaaaag attaactacg taggagacgt tctattttgc tgttttccaa cttacagaag     1980

attatgtact tagaaagact tttagtagaa aaatgtctat caaagttagc tactcccact     2040

gtctctgtgc tagaaaattt taagtttaga aatgtaactt ttgttttaat acggcattcc     2100

aaacataact agtgctcatg agtgatagga gttcagggtt tacagattcc tctaaggcgg     2160

ggtctctgac tggagcagtc ttctaaaatg cctgttatgt agtgcaatta gtaaataaag     2220

ggtgatgtcc accacgaact tgggcacagg gtctataaaa tgtgttttct tcaggcatga     2280

atgaatcaac ctctaagatc cgctccaact ctgcatatct gaaaactgca ggtaagaaga     2340

gaggaataaa gtctattcat ttagcctggt ctgttttcag ttgttctgga gtcttttctc     2400

tcccaagttt gttaagttag ataggtttct atatttcaaa agcttgaaac tcaaacaaaa     2460

gtccacaggg aatctggaag tcaagactcc aggaaaagaa tccagaccag aactaggatg     2520

acagggaatt acattttaag caaggaaagc tttgacaaag ccaattttac tgaactagtt     2580

cagtgtgaat ttggtcattt gctgttctct ggtataatat atactgcata gcactcattt     2640

cacctgcatg agactatttt cttactcgcc ttaccagaac atgagaaacc ttcccacaaa     2700

cctccctggc atttgtgcac tctctccaaa cttggtcaca aagaatgaag aggacagtac     2760

tagaggagga atattacgcc tgtgaaatgt tgacaatgcc aagtgaactt tgtagctata     2820

ctgacttact tttcaagata aaaaaaaaat catgtgcata tgatatatat tattgtgcta     2880

gcatttgcac agaggtagtt ttagaataag acaaagttta acagctgtta tcactgagca     2940

atgaggcaga agaggaaagt gggcttgcac tcactactaa tcagctagag atcaggttaa     3000

ctgagaggag attccaagat tggacccagg gcctcctgca tgctgagtgt gtgctctacc     3060

cctgggttgc atctccagct cttcattaga attttttaaa cgaacatatt acttttataa     3120

taatgaaagt gaatttcttt ctatttcctg ttattcatgc ataacaggat tcacagaaga     3180

cacttcctct tcggacacat ttggaaacac aaggcagtaa gccttacttt acagttagcc     3240

ctcttgccta agagaaagcc gagggggaag aaagagagca aagctttcag aaaaggaatc     3300

cattaaagct acagagatgc ttcgaagtta gaacaccatg aaacaaagaa gcactaagag     3360

gtctaagacg taaaccctcc ccaaaaagcc aagcctacaa taaactgaaa ttacaaagta     3420

attctgtgat gttcgtacct cataaagttt tagatcctca cctacgcaaa catactttgc     3480

agtcgctctt tagaaaaggg caaggtacaa tggaaggaga gacaggactg tgaccaggaa     3540

attaaaatgg attacaatta gggtgtggta gaagggaagg gaacaaaaga aaagttcaga     3600

att                                                                   3603


<210>  14
<211>  3603
<212>  DNA
<213>  artificial

<220>
<223>  EASE, complementary sequence

<400>  14
aattctgaac ttttcttttg ttcccttccc ttctaccaca ccctaattgt aatccatttt       60

aatttcctgg tcacagtcct gtctctcctt ccattgtacc ttgccctttt ctaaagagcg      120

actgcaaagt atgtttgcgt aggtgaggat ctaaaacttt atgaggtacg aacatcacag      180

aattactttg taatttcagt ttattgtagg cttggctttt tggggagggt ttacgtctta      240

gacctcttag tgcttctttg tttcatggtg ttctaacttc gaagcatctc tgtagcttta      300

atggattcct tttctgaaag ctttgctctc tttcttcccc ctcggctttc tcttaggcaa      360

gagggctaac tgtaaagtaa ggcttactgc cttgtgtttc caaatgtgtc cgaagaggaa      420

gtgtcttctg tgaatcctgt tatgcatgaa taacaggaaa tagaaagaaa ttcactttca      480

ttattataaa agtaatatgt tcgtttaaaa aattctaatg aagagctgga gatgcaaccc      540

aggggtagag cacacactca gcatgcagga ggccctgggt ccaatcttgg aatctcctct      600

cagttaacct gatctctagc tgattagtag tgagtgcaag cccactttcc tcttctgcct      660

cattgctcag tgataacagc tgttaaactt tgtcttattc taaaactacc tctgtgcaaa      720

tgctagcaca ataatatata tcatatgcac atgatttttt ttttatcttg aaaagtaagt      780

cagtatagct acaaagttca cttggcattg tcaacatttc acaggcgtaa tattcctcct      840

ctagtactgt cctcttcatt ctttgtgacc aagtttggag agagtgcaca aatgccaggg      900

aggtttgtgg gaaggtttct catgttctgg taaggcgagt aagaaaatag tctcatgcag      960

gtgaaatgag tgctatgcag tatatattat accagagaac agcaaatgac caaattcaca     1020

ctgaactagt tcagtaaaat tggctttgtc aaagctttcc ttgcttaaaa tgtaattccc     1080

tgtcatccta gttctggtct ggattctttt cctggagtct tgacttccag attccctgtg     1140

gacttttgtt tgagtttcaa gcttttgaaa tatagaaacc tatctaactt aacaaacttg     1200

ggagagaaaa gactccagaa caactgaaaa cagaccaggc taaatgaata gactttattc     1260

ctctcttctt acctgcagtt ttcagatatg cagagttgga gcggatctta gaggttgatt     1320

cattcatgcc tgaagaaaac acattttata gaccctgtgc ccaagttcgt ggtggacatc     1380

accctttatt tactaattgc actacataac aggcatttta gaagactgct ccagtcagag     1440

accccgcctt agaggaatct gtaaaccctg aactcctatc actcatgagc actagttatg     1500

tttggaatgc cgtattaaaa caaaagttac atttctaaac ttaaaatttt ctagcacaga     1560

gacagtggga gtagctaact ttgatagaca tttttctact aaaagtcttt ctaagtacat     1620

aatcttctgt aagttggaaa acagcaaaat agaacgtctc ctacgtagtt aatctttttg     1680

cataatttgc acatgtagga gttattagta tacgggtaag ttttcacttt ttcccccaac     1740

tggagtgtct tgtggctggg tttgaaaaag ggaacgggag gccgctggag gggattggta     1800

aatgagataa aacaccactc attcaactca gtgactcagc atttaaattt tccataaaag     1860

gattaaagga aaattaaaca aattcttaaa gccaagactc tggagaaact tgttggtgtg     1920

ctttagtttt cactgttatg actcatgaat ttatgcataa attagtacat ttataaaaac     1980

atagcctttt tagagttttc tgtttggcta aagtgccatt gttagcattt ggaattacct     2040

ttttatgtct tatatttttt ccaaataaaa ataaatgttt ctgctgtctt actactgaaa     2100

ctacgttgtg agcactttaa atttctcaaa gcagtttcgc ctgttatact tggcgcttag     2160

tcatcgtcgt acacaacagg acctgattaa gaaggctgtg ctgcctctaa gccgggctag     2220

attgtagcca ctagcaacca ggctgcaata atttcccttt gatgacatca tccactgtgg     2280

aagaacccag ttgcttcagc cagtcgaact atacagttcc aacctcatca aatatggcat     2340

ctcccttgcc tgctatagca gggggaggaa aaaatgccac catcttttta atctagcaag     2400

cttctctttt cttcatcttt ttttttttct tttaaaaaaa ttctgatcat ggatgcttct     2460

tccgatccct atttgcctta tgacggggga ggagacaata tccccttgag ggaattacat     2520

aaaagaggta agagcatccc cttgctctga atcctctgtt ggttgttgtg catgcggctg     2580

ggcggttctg gggacaggct gtctgttgtc ctcttgctgc aatgtgctgc ttagttgccc     2640

tgccttgttg ctgtgggaga atgcgacctt cccagcaggg ctggccctcc ctgattgttt     2700

gctctgtgca gattagccct gcttcagatc acatagggct gcagactcca tcttctgtgt     2760

gaaaatgctt tcggtttgat tgcagaaata agctgccttt acagccagct aaagtcctgg     2820

tggttggttg gcacctgcaa agtagtattt ttgtacctct ggaaacttat attttcttta     2880

cacagcaata tcaagtgccg gtatgccatt ctgttttggc tgctgccaat taccatgtag     2940

actttgcacc acagagtaat agtaaaagct cctagctgca ttttataaca tttaaaaata     3000

gcaggaaaga agaattattt ttgatttaac atgtttttgt catttaacgt cttaactgat     3060

tgacatacta tattgtctgt ctcgtgggta tcttgtacaa cttgatagga taaagcaatt     3120

tagttttttt tttttttttt aaatacatcc agaatgtaag tcgtcagtag ttttcgaaca     3180

gataagtaat ggtgttaatc ttttggcagg ctttgccttg gtctccttaa agctaattag     3240

gtgttactta attaaactgc tcttttgctc attttcttaa attatttttt taaaagatag     3300

ttggcatttg ctgttctaga aataaacttc aagaaacatt ctttagccag atgacttcat     3360

gtatgagcca tgttagtttg aattatttgc ttggtgttat aaactttatg gtttaatacc     3420

aacttttatt atgtttacaa ggtaaataag gaaaatttca agtacatttt gtatcctgag     3480

aacaaattta agttccatag aatttaggaa ttacaatgta ttcaacagat acttacttgt     3540

catactgtgc ctgcaaaaca ataattagac tctgaacagg tgcaacaatt ttctgtagaa     3600

ttg                                                                   3603


<210>  15
<211>  3608
<212>  DNA
<213>  artificial

<220>
<223>  EASE

<400>  15
gatctaattc tgaacttttc ttttgttccc ttcccttcta ccacacccta attgtaatcc       60

attttaattt cctggtcaca gtcctgtctc tccttccatt gtaccttgcc cttttctaaa      120

gagcgactgc aaagtatgtt tgcgtaggtg aggatctaaa actttatgag gtacgaacat      180

cacagaatta ctttgtaatt tcagtttatt gtaggcttgg ctttttgggg agggtttacg      240

tcttagacct cttagtgctt ctttgtttca tggtgttcta acttcgaagc atctctgtag      300

ctttaatgga ttccttttct gaaagctttg ctctctttct tccccctcgg ctttctctta      360

ggcaagaggg ctaactgtaa agtaaggctt actgccttgt gtttccaaat gtgtccgaag      420

aggaagtgtc ttctgtgaat cctgttatgc atgaataaca ggaaatagaa agaaattcac      480

tttcattatt ataaaagtaa tatgttcgtt taaaaaattc taatgaagag ctggagatgc      540

aacccagggg tagagcacac actcagcatg caggaggccc tgggtccaat cttggaatct      600

cctctcagtt aacctgatct ctagctgatt agtagtgagt gcaagcccac tttcctcttc      660

tgcctcattg ctcagtgata acagctgtta aactttgtct tattctaaaa ctacctctgt      720

gcaaatgcta gcacaataat atatatcata tgcacatgat ttttttttta tcttgaaaag      780

taagtcagta tagctacaaa gttcacttgg cattgtcaac atttcacagg cgtaatattc      840

ctcctctagt actgtcctct tcattctttg tgaccaagtt tggagagagt gcacaaatgc      900

cagggaggtt tgtgggaagg tttctcatgt tctggtaagg cgagtaagaa aatagtctca      960

tgcaggtgaa atgagtgcta tgcagtatat attataccag agaacagcaa atgaccaaat     1020

tcacactgaa ctagttcagt aaaattggct ttgtcaaagc tttccttgct taaaatgtaa     1080

ttccctgtca tcctagttct ggtctggatt cttttcctgg agtcttgact tccagattcc     1140

ctgtggactt ttgtttgagt ttcaagcttt tgaaatatag aaacctatct aacttaacaa     1200

acttgggaga gaaaagactc cagaacaact gaaaacagac caggctaaat gaatagactt     1260

tattcctctc ttcttacctg cagttttcag atatgcagag ttggagcgga tcttagaggt     1320

tgattcattc atgcctgaag aaaacacatt ttatagaccc tgtgcccaag ttcgtggtgg     1380

acatcaccct ttatttacta attgcactac ataacaggca ttttagaaga ctgctccagt     1440

cagagacccc gccttagagg aatctgtaaa ccctgaactc ctatcactca tgagcactag     1500

ttatgtttgg aatgccgtat taaaacaaaa gttacatttc taaacttaaa attttctagc     1560

acagagacag tgggagtagc taactttgat agacattttt ctactaaaag tctttctaag     1620

tacataatct tctgtaagtt ggaaaacagc aaaatagaac gtctcctacg tagttaatct     1680

ttttgcataa tttgcacatg taggagttat tagtatacgg gtaagttttc actttttccc     1740

ccaactggag tgtcttgtgg ctgggtttga aaaagggaac gggaggccgc tggaggggat     1800

tggtaaatga gataaaacac cactcattca actcagtgac tcagcattta aattttccat     1860

aaaaggatta aaggaaaatt aaacaaattc ttaaagccaa gactctggag aaacttgttg     1920

gtgtgcttta gttttcactg ttatgactca tgaatttatg cataaattag tacatttata     1980

aaaacatagc ctttttagag ttttctgttt ggctaaagtg ccattgttag catttggaat     2040

taccttttta tgtcttatat tttttccaaa taaaaataaa tgtttctgct gtcttactac     2100

tgaaactacg ttgtgagcac tttaaatttc tcaaagcagt ttcgcctgtt atacttggcg     2160

cttagtcatc gtcgtacaca acaggacctg attaagaagg ctgtgctgcc tctaagccgg     2220

gctagattgt agccactagc aaccaggctg caataatttc cctttgatga catcatccac     2280

tgtggaagaa cccagttgct tcagccagtc gaactataca gttccaacct catcaaatat     2340

ggcatctccc ttgcctgcta tagcaggggg aggaaaaaat gccaccatct ttttaatcta     2400

gcaagcttct cttttcttca tctttttttt tttcttttaa aaaaattctg atcatggatg     2460

cttcttccga tccctatttg ccttatgacg ggggaggaga caatatcccc ttgagggaat     2520

tacataaaag aggtaagagc atccccttgc tctgaatcct ctgttggttg ttgtgcatgc     2580

ggctgggcgg ttctggggac aggctgtctg ttgtcctctt gctgcaatgt gctgcttagt     2640

tgccctgcct tgttgctgtg ggagaatgcg accttcccag cagggctggc cctccctgat     2700

tgtttgctct gtgcagatta gccctgcttc agatcacata gggctgcaga ctccatcttc     2760

tgtgtgaaaa tgctttcggt ttgattgcag aaataagctg cctttacagc cagctaaagt     2820

cctggtggtt ggttggcacc tgcaaagtag tatttttgta cctctggaaa cttatatttt     2880

ctttacacag caatatcaag tgccggtatg ccattctgtt ttggctgctg ccaattacca     2940

tgtagacttt gcaccacaga gtaatagtaa aagctcctag ctgcatttta taacatttaa     3000

aaatagcagg aaagaagaat tatttttgat ttaacatgtt tttgtcattt aacgtcttaa     3060

ctgattgaca tactatattg tctgtctcgt gggtatcttg tacaacttga taggataaag     3120

caatttagtt tttttttttt tttttaaata catccagaat gtaagtcgtc agtagttttc     3180

gaacagataa gtaatggtgt taatcttttg gcaggctttg ccttggtctc cttaaagcta     3240

attaggtgtt acttaattaa actgctcttt tgctcatttt cttaaattat ttttttaaaa     3300

gatagttggc atttgctgtt ctagaaataa acttcaagaa acattcttta gccagatgac     3360

ttcatgtatg agccatgtta gtttgaatta tttgcttggt gttataaact ttatggttta     3420

ataccaactt ttattatgtt tacaaggtaa ataaggaaaa tttcaagtac attttgtatc     3480

ctgagaacaa atttaagttc catagaattt aggaattaca atgtattcaa cagatactta     3540

cttgtcatac tgtgcctgca aaacaataat tagactctga acaggtgcaa caattttctg     3600

tagaattg                                                              3608


<210>  16
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  T7 promoter

<400>  16
taatacgact cactatagg                                                    19


