                         SEQUENCE LISTING

<110>  Molecular Partners AG
 
<120>  Designed ankyrin repeat domain with improved stability

<130>  P5754PC00

<150>  EP19178282.0
<151>  2019-06-04

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  PRT
<213>  His-tag

<400>  1

Met Arg Gly Ser His His His His His His Gly Ser 
1               5                   10          


<210>  2
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ankyrin repeat domain

<400>  2

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asn Glu Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 


<210>  3
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ankyrin repeat domain

<400>  3

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 


<210>  4
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ankyrin repeat domain

<400>  4

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asp Ala Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 


<210>  5
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ankyrin repeat domain

<400>  5

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ile Asp Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 


<210>  6
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ankyrin repeat domain

<400>  6

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asp Asp Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
        115                 120                 


<210>  7
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein comprising four ankyrin repeat domains

<400>  7

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asn Glu Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 


Pro Thr Gly Ser Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala 
145                 150                 155                 160 


Gly Gln Asp Asp Glu Val Arg Ile Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     


Asn Ala Lys Asp Arg Tyr Gly Asp Thr Pro Leu His Leu Ala Ala Asp 
            180                 185                 190         


Ile Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             


Val Asn Ala Glu Asp Tyr Phe Gly Asn Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 


Ser Tyr Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 


Asp Val Asn Ala Lys Asp Asp Tyr Gly Asn Thr Pro Leu His Leu Ala 
                245                 250                 255     


Ala Asn Thr Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly 
            260                 265                 270         


Ala Asp Val Asn Ala Gln Asp Lys Ser Gly Lys Thr Pro Ala Asp Leu 
        275                 280                 285             


Ala Ala Asp Ala Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala 
    290                 295                 300                 


Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr 
305                 310                 315                 320 


Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Asp Lys Lys Leu Leu 
                325                 330                 335     


Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Ile Leu Leu Lys 
            340                 345                 350         


Ala Gly Ala Asp Val Asn Ala Lys Asp Ser Thr Gly Trp Thr Pro Leu 
        355                 360                 365             


His Leu Ala Ala Pro Trp Gly His Pro Glu Ile Val Glu Val Leu Leu 
    370                 375                 380                 


Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Gln Gly Trp Thr Pro 
385                 390                 395                 400 


Leu His Leu Ala Ala Ala Ala Gly His Leu Glu Ile Val Glu Val Leu 
                405                 410                 415     


Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Lys Ser Gly Lys Thr 
            420                 425                 430         


Pro Ala Asp Leu Ala Ala Asp Ala Gly His Glu Asp Ile Ala Glu Val 
        435                 440                 445             


Leu Gln Lys Ala Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr 
    450                 455                 460                 


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly 
465                 470                 475                 480 


Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg 
                485                 490                 495     


Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Tyr Phe Ser 
            500                 505                 510         


His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu Lys Ile Val 
        515                 520                 525             


Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Ala 
    530                 535                 540                 


Gly Lys Thr Pro Leu His Leu Ala Ala Asn Glu Gly His Leu Glu Ile 
545                 550                 555                 560 


Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Ile 
                565                 570                 575     


Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly His Glu Asp 
            580                 585                 590         


Ile Ala Glu Val Leu Gln Lys Ala Ala 
        595                 600     


<210>  8
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein comprising four ankyrin repeat domains

<400>  8

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asn Glu Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 


Pro Thr Gly Ser Asp Leu Gly Ile Lys Leu Leu Phe Ala Ala Ala Lys 
145                 150                 155                 160 


Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     


Asn Ala Lys Asp Phe Gln Gly Val Thr Pro Leu His Ile Ala Ala Gln 
            180                 185                 190         


Ser Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             


Val Asn Ala Lys Asp Val Thr Gly Asp Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 


Gln His Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 


Asp Val Asn Ala Gln Asp Glu Arg Gly Trp Thr Pro Ala Asp Leu Ala 
                245                 250                 255     


Ala Asp Trp Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            260                 265                 270         


Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr 
        275                 280                 285             


Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly Lys Lys Leu Leu Glu 
    290                 295                 300                 


Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala 
305                 310                 315                 320 


Gly Ala Asp Val Asn Ala Lys Asp Glu Tyr Gly Leu Thr Pro Leu Tyr 
                325                 330                 335     


Leu Ala Thr Ala His Gly His Leu Glu Ile Val Glu Val Leu Leu Lys 
            340                 345                 350         


Ala Gly Ala Asp Val Asn Ala Val Asp Ala Ile Gly Phe Thr Pro Leu 
        355                 360                 365             


His Leu Ala Ala Phe Ile Gly His Leu Glu Ile Ala Glu Val Leu Leu 
    370                 375                 380                 


Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Lys Phe Gly Lys Thr Pro 
385                 390                 395                 400 


Ala Asp Ile Ala Ala Gly Ala Gly Asn Glu Asp Ile Ala Glu Val Leu 
                405                 410                 415     


Gln Lys Ala Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro 
            420                 425                 430         


Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly Lys 
        435                 440                 445             


Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Glu 
    450                 455                 460                 


Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Tyr Phe Ser His 
465                 470                 475                 480 


Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu Lys Ile Val Glu 
                485                 490                 495     


Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Ala Gly 
            500                 505                 510         


Lys Thr Pro Leu His Leu Ala Ala Asn Glu Gly His Leu Glu Ile Val 
        515                 520                 525             


Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Ile Phe 
    530                 535                 540                 


Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly His Glu Asp Ile 
545                 550                 555                 560 


Ala Glu Val Leu Gln Lys Ala Ala 
                565             


<210>  9
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein comprising four ankyrin repeat domains

<400>  9

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 


Pro Thr Gly Ser Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala 
145                 150                 155                 160 


Gly Gln Asp Asp Glu Val Arg Ile Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     


Asn Ala Lys Asp Arg Tyr Gly Asp Thr Pro Leu His Leu Ala Ala Asp 
            180                 185                 190         


Ile Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             


Val Asn Ala Glu Asp Tyr Phe Gly Asn Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 


Ser Tyr Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 


Asp Val Asn Ala Lys Asp Asp Tyr Gly Asn Thr Pro Leu His Leu Ala 
                245                 250                 255     


Ala Asn Thr Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly 
            260                 265                 270         


Ala Asp Val Asn Ala Gln Asp Lys Ser Gly Lys Thr Pro Ala Asp Leu 
        275                 280                 285             


Ala Ala Asp Ala Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala 
    290                 295                 300                 


Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr 
305                 310                 315                 320 


Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Asp Lys Lys Leu Leu 
                325                 330                 335     


Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Ile Leu Leu Lys 
            340                 345                 350         


Ala Gly Ala Asp Val Asn Ala Lys Asp Ser Thr Gly Trp Thr Pro Leu 
        355                 360                 365             


His Leu Ala Ala Pro Trp Gly His Pro Glu Ile Val Glu Val Leu Leu 
    370                 375                 380                 


Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Gln Gly Trp Thr Pro 
385                 390                 395                 400 


Leu His Leu Ala Ala Ala Ala Gly His Leu Glu Ile Val Glu Val Leu 
                405                 410                 415     


Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Lys Ser Gly Lys Thr 
            420                 425                 430         


Pro Ala Asp Leu Ala Ala Asp Ala Gly His Glu Asp Ile Ala Glu Val 
        435                 440                 445             


Leu Gln Lys Ala Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr 
    450                 455                 460                 


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly 
465                 470                 475                 480 


Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg 
                485                 490                 495     


Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Tyr Phe Ser 
            500                 505                 510         


His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu Lys Ile Val 
        515                 520                 525             


Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Ala 
    530                 535                 540                 


Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His Leu Glu Ile 
545                 550                 555                 560 


Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Ile 
                565                 570                 575     


Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly His Glu Asp 
            580                 585                 590         


Ile Ala Glu Val Leu Gln Lys Ala Ala 
        595                 600     


<210>  10
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein comprising four ankyrin repeat domains

<400>  10

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 


Pro Thr Gly Ser Asp Leu Gly Ile Lys Leu Leu Phe Ala Ala Ala Lys 
145                 150                 155                 160 


Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     


Asn Ala Lys Asp Phe Gln Gly Val Thr Pro Leu His Ile Ala Ala Gln 
            180                 185                 190         


Ser Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             


Val Asn Ala Lys Asp Val Thr Gly Asp Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 


Gln His Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 


Asp Val Asn Ala Gln Asp Glu Arg Gly Trp Thr Pro Ala Asp Leu Ala 
                245                 250                 255     


Ala Asp Trp Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            260                 265                 270         


Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr 
        275                 280                 285             


Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly Lys Lys Leu Leu Glu 
    290                 295                 300                 


Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala 
305                 310                 315                 320 


Gly Ala Asp Val Asn Ala Lys Asp Glu Tyr Gly Leu Thr Pro Leu Tyr 
                325                 330                 335     


Leu Ala Thr Ala His Gly His Leu Glu Ile Val Glu Val Leu Leu Lys 
            340                 345                 350         


Ala Gly Ala Asp Val Asn Ala Val Asp Ala Ile Gly Phe Thr Pro Leu 
        355                 360                 365             


His Leu Ala Ala Phe Ile Gly His Leu Glu Ile Ala Glu Val Leu Leu 
    370                 375                 380                 


Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Lys Phe Gly Lys Thr Pro 
385                 390                 395                 400 


Ala Asp Ile Ala Ala Gly Ala Gly Asn Glu Asp Ile Ala Glu Val Leu 
                405                 410                 415     


Gln Lys Ala Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro 
            420                 425                 430         


Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly Lys 
        435                 440                 445             


Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Glu 
    450                 455                 460                 


Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Tyr Phe Ser His 
465                 470                 475                 480 


Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu Lys Ile Val Glu 
                485                 490                 495     


Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Ala Gly 
            500                 505                 510         


Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly His Leu Glu Ile Val 
        515                 520                 525             


Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Ile Phe 
    530                 535                 540                 


Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly His Glu Asp Ile 
545                 550                 555                 560 


Ala Glu Val Leu Gln Lys Ala Ala 
                565             


<210>  11
<211>  601
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein comprising four ankyrin repeat domains

<400>  11

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asp Ala Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 


Pro Thr Gly Ser Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala 
145                 150                 155                 160 


Gly Gln Asp Asp Glu Val Arg Ile Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     


Asn Ala Lys Asp Arg Tyr Gly Asp Thr Pro Leu His Leu Ala Ala Asp 
            180                 185                 190         


Ile Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             


Val Asn Ala Glu Asp Tyr Phe Gly Asn Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 


Ser Tyr Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 


Asp Val Asn Ala Lys Asp Asp Tyr Gly Asn Thr Pro Leu His Leu Ala 
                245                 250                 255     


Ala Asn Thr Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly 
            260                 265                 270         


Ala Asp Val Asn Ala Gln Asp Lys Ser Gly Lys Thr Pro Ala Asp Leu 
        275                 280                 285             


Ala Ala Asp Ala Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala 
    290                 295                 300                 


Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr 
305                 310                 315                 320 


Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Asp Lys Lys Leu Leu 
                325                 330                 335     


Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Ile Leu Leu Lys 
            340                 345                 350         


Ala Gly Ala Asp Val Asn Ala Lys Asp Ser Thr Gly Trp Thr Pro Leu 
        355                 360                 365             


His Leu Ala Ala Pro Trp Gly His Pro Glu Ile Val Glu Val Leu Leu 
    370                 375                 380                 


Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Gln Gly Trp Thr Pro 
385                 390                 395                 400 


Leu His Leu Ala Ala Ala Ala Gly His Leu Glu Ile Val Glu Val Leu 
                405                 410                 415     


Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Lys Ser Gly Lys Thr 
            420                 425                 430         


Pro Ala Asp Leu Ala Ala Asp Ala Gly His Glu Asp Ile Ala Glu Val 
        435                 440                 445             


Leu Gln Lys Ala Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr 
    450                 455                 460                 


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly 
465                 470                 475                 480 


Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg 
                485                 490                 495     


Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Tyr Phe Ser 
            500                 505                 510         


His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu Lys Ile Val 
        515                 520                 525             


Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Ala 
    530                 535                 540                 


Gly Lys Thr Pro Leu His Leu Ala Ala Asp Ala Gly His Leu Glu Ile 
545                 550                 555                 560 


Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Ile 
                565                 570                 575     


Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly His Glu Asp 
            580                 585                 590         


Ile Ala Glu Val Leu Gln Lys Ala Ala 
        595                 600     


<210>  12
<211>  568
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein comprising four ankyrin repeat domains

<400>  12

Asp Leu Gly Lys Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp 
1               5                   10                  15      


Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp 
            20                  25                  30          


Tyr Phe Ser His Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu 
        35                  40                  45              


Lys Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys 
    50                  55                  60                  


Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asp Ala Gly His 
65                  70                  75                  80  


Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala 
                85                  90                  95      


Gln Asp Ile Phe Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly 
            100                 105                 110         


His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala Gly Ser Pro Thr 
        115                 120                 125             


Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr 
    130                 135                 140                 


Pro Thr Gly Ser Asp Leu Gly Ile Lys Leu Leu Phe Ala Ala Ala Lys 
145                 150                 155                 160 


Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala Gly Ala Asp Val 
                165                 170                 175     


Asn Ala Lys Asp Phe Gln Gly Val Thr Pro Leu His Ile Ala Ala Gln 
            180                 185                 190         


Ser Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala Asp 
        195                 200                 205             


Val Asn Ala Lys Asp Val Thr Gly Asp Thr Pro Leu His Leu Ala Ala 
    210                 215                 220                 


Gln His Gly His Leu Glu Ile Val Glu Val Leu Leu Lys Ala Gly Ala 
225                 230                 235                 240 


Asp Val Asn Ala Gln Asp Glu Arg Gly Trp Thr Pro Ala Asp Leu Ala 
                245                 250                 255     


Ala Asp Trp Gly His Glu Asp Ile Ala Glu Val Leu Gln Lys Ala Ala 
            260                 265                 270         


Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Thr 
        275                 280                 285             


Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly Lys Lys Leu Leu Glu 
    290                 295                 300                 


Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Glu Leu Leu Lys Ala 
305                 310                 315                 320 


Gly Ala Asp Val Asn Ala Lys Asp Glu Tyr Gly Leu Thr Pro Leu Tyr 
                325                 330                 335     


Leu Ala Thr Ala His Gly His Leu Glu Ile Val Glu Val Leu Leu Lys 
            340                 345                 350         


Ala Gly Ala Asp Val Asn Ala Val Asp Ala Ile Gly Phe Thr Pro Leu 
        355                 360                 365             


His Leu Ala Ala Phe Ile Gly His Leu Glu Ile Ala Glu Val Leu Leu 
    370                 375                 380                 


Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Lys Phe Gly Lys Thr Pro 
385                 390                 395                 400 


Ala Asp Ile Ala Ala Gly Ala Gly Asn Glu Asp Ile Ala Glu Val Leu 
                405                 410                 415     


Gln Lys Ala Ala Gly Ser Pro Thr Pro Thr Pro Thr Thr Pro Thr Pro 
            420                 425                 430         


Thr Pro Thr Thr Pro Thr Pro Thr Pro Thr Gly Ser Asp Leu Gly Lys 
        435                 440                 445             


Lys Leu Leu Glu Ala Ala Arg Ala Gly Gln Asp Asp Glu Val Arg Glu 
    450                 455                 460                 


Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Tyr Phe Ser His 
465                 470                 475                 480 


Thr Pro Leu His Leu Ala Ala Arg Asn Gly His Leu Lys Ile Val Glu 
                485                 490                 495     


Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Lys Asp Phe Ala Gly 
            500                 505                 510         


Lys Thr Pro Leu His Leu Ala Ala Asp Ala Gly His Leu Glu Ile Val 
        515                 520                 525             


Glu Val Leu Leu Lys Ala Gly Ala Asp Val Asn Ala Gln Asp Ile Phe 
    530                 535                 540                 


Gly Lys Thr Pro Ala Asp Ile Ala Ala Asp Ala Gly His Glu Asp Ile 
545                 550                 555                 560 


Ala Glu Val Leu Gln Lys Ala Ala 
                565             


<210>  13
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence motif


<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  X represents an amino acid residue selected from the group 
       consisting of A, D, and I; preferably, A and D

<220>
<221>  MISC_FEATURE
<222>  (15)..(15)
<223>  X represents an amino acid residue selected from the group 
       consisting of A and D

<400>  13

Lys Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Xaa Xaa Gly 
1               5                   10                  15      


<210>  14
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence motif

<400>  14

Lys Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Ala Asp Gly 
1               5                   10                  15      


<210>  15
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence motif

<400>  15

Lys Asp Phe Ala Gly Lys Thr Pro Leu His Leu Ala Ala Asp Ala Gly 
1               5                   10                  15      


